AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016380.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016380.01.T01 MTR_3g088400 92.941 170 9 2 1 168 1 169 3.51e-95 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016380.01.T01 AT1G77400 32.203 236 85 9 2 168 3 232 1.79e-15 71.6

Find 65 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTGAATGAGGGAGATTC+CGG 0.280355 3:+82683123 None:intergenic
CATTTGAATGGAACTGATCC+TGG 0.357724 3:+82683195 None:intergenic
ACGGCCCCTTCTTGCGCAAC+CGG 0.388573 3:-82682995 MsG0380016380.01.T01:CDS
CGCACTCCGGTTGCGCAAGA+AGG 0.392092 3:+82682989 None:intergenic
CAGGATCAGTTCCATTCAAA+TGG 0.392591 3:-82683194 MsG0380016380.01.T01:CDS
GGTTTGATTTCCCATTTGAA+TGG 0.399102 3:+82683183 None:intergenic
TGATGATGGTGGTGAGGAAT+GGG 0.403240 3:+82683153 None:intergenic
CTAAGTCCTTGTCGCCGTTC+CGG 0.421562 3:-82682828 MsG0380016380.01.T01:CDS
CTCCGTAGCCGCTTCAGAAA+AGG 0.423405 3:+82682940 None:intergenic
TTCTGAAGCGGCTACGGAGC+AGG 0.428600 3:-82682936 MsG0380016380.01.T01:CDS
TCCAGTTCCGATGTATAATC+AGG 0.433268 3:+82682886 None:intergenic
CCGGAGTGCGTGACTTCGTC+AGG 0.434551 3:-82682976 MsG0380016380.01.T01:CDS
TTGGTGATGCTGAATGGGCT+TGG 0.438084 3:-82682759 MsG0380016380.01.T01:CDS
GCTTGAATGAGGGAGATTCC+GGG 0.452305 3:+82683124 None:intergenic
ACCTGATTATACATCGGAAC+TGG 0.454932 3:-82682887 MsG0380016380.01.T01:CDS
ATGATGATGGTGGTGAGGAA+TGG 0.456155 3:+82683152 None:intergenic
TGGTGAGGAATGGGGAGTCC+TGG 0.459210 3:+82683162 None:intergenic
CTCCTTCCGGTCAAGTCCTA+AGG 0.468786 3:-82683043 MsG0380016380.01.T01:CDS
ACCCATTGGTGATGCTGAAT+GGG 0.470201 3:-82682764 MsG0380016380.01.T01:CDS
ATTCCCGTACACGCTCCTTC+CGG 0.478158 3:-82683056 MsG0380016380.01.T01:CDS
GATGCTGAATGGGCTTGGTT+CGG 0.481588 3:-82682754 MsG0380016380.01.T01:CDS
GCACTCCGGTTGCGCAAGAA+GGG 0.484553 3:+82682990 None:intergenic
CATCTCTCAGAACCGACCTT+AGG 0.491967 3:+82683027 None:intergenic
GCCCATTCAGCATCACCAAT+GGG 0.491981 3:+82682763 None:intergenic
ACATCGGAACTGGAGACAAT+TGG 0.496458 3:-82682877 MsG0380016380.01.T01:CDS
AACCCATTGGTGATGCTGAA+TGG 0.502313 3:-82682765 MsG0380016380.01.T01:CDS
TTGACCGGAAGGAGCGTGTA+CGG 0.502706 3:+82683052 None:intergenic
TAGGAGCCGAGTTGCGGTGG+AGG 0.502809 3:+82683096 None:intergenic
TGGAGGTTTGAGCTTGAATG+AGG 0.505731 3:+82683113 None:intergenic
CTGAGAGATGGCGGTTTGAA+CGG 0.517905 3:-82683014 MsG0380016380.01.T01:CDS
GATGATTCATTCAAGAAGCC+AGG 0.528926 3:-82683213 MsG0380016380.01.T01:CDS
CAAATTCAAAACAATGGTTG+TGG 0.535320 3:-82683241 None:intergenic
ATAGAAGACGACACGGTTGA+AGG 0.536946 3:+82682802 None:intergenic
AGGATCAGTTCCATTCAAAT+GGG 0.539229 3:-82683193 MsG0380016380.01.T01:CDS
CTCCTACAAGTTATCTCCGG+TGG 0.542586 3:-82683079 MsG0380016380.01.T01:CDS
TGACCGGAAGGAGCGTGTAC+GGG 0.549214 3:+82683053 None:intergenic
TCAGCATCACCAATGGGTTG+TGG 0.553882 3:+82682769 None:intergenic
CGGCTCCTACAAGTTATCTC+CGG 0.555895 3:-82683082 MsG0380016380.01.T01:CDS
CTAAGGTCGGTTCTGAGAGA+TGG 0.555995 3:-82683026 MsG0380016380.01.T01:CDS
AACCGACCTTAGGACTTGAC+CGG 0.564275 3:+82683037 None:intergenic
ACGGTTGAAGGCCGCCGGAA+CGG 0.564700 3:+82682814 None:intergenic
TTGTAGGAGCCGAGTTGCGG+TGG 0.564820 3:+82683093 None:intergenic
AGCCCATTCAGCATCACCAA+TGG 0.565303 3:+82682762 None:intergenic
AACTTGTAGGAGCCGAGTTG+CGG 0.574364 3:+82683090 None:intergenic
TTCAAATGGGAAATCAAACC+AGG 0.594700 3:-82683180 MsG0380016380.01.T01:CDS
CACTCCGGTTGCGCAAGAAG+GGG 0.595751 3:+82682991 None:intergenic
ATCCACCGGAGATAACTTGT+AGG 0.597714 3:+82683077 None:intergenic
GGAGGTTTGAGCTTGAATGA+GGG 0.597771 3:+82683114 None:intergenic
GATGATGGTGGTGAGGAATG+GGG 0.602221 3:+82683154 None:intergenic
AGGCCGCCGGAACGGCGACA+AGG 0.608271 3:+82682822 None:intergenic
CTCAAACCTCCACCGCAACT+CGG 0.610739 3:-82683102 MsG0380016380.01.T01:CDS
TTCCGGTCAAGTCCTAAGGT+CGG 0.611011 3:-82683039 MsG0380016380.01.T01:CDS
ATTCAAAACAATGGTTGTGG+CGG 0.614374 3:-82683238 None:intergenic
CCACCATCATCATCAAAACC+CGG 0.614647 3:-82683142 MsG0380016380.01.T01:CDS
GAGCGTGTACGGGAATCCAC+CGG 0.615955 3:+82683063 None:intergenic
ACGACACGGTTGAAGGCCGC+CGG 0.618792 3:+82682809 None:intergenic
GCGTCGTCACCACAACCCAT+TGG 0.619177 3:-82682778 MsG0380016380.01.T01:CDS
CCTGACGAAGTCACGCACTC+CGG 0.625012 3:+82682976 None:intergenic
AGTCCTTGTCGCCGTTCCGG+CGG 0.638705 3:-82682825 MsG0380016380.01.T01:CDS
GACCTTAGGACTTGACCGGA+AGG 0.639720 3:+82683041 None:intergenic
AGGTCGGTTCTGAGAGATGG+CGG 0.643746 3:-82683023 MsG0380016380.01.T01:CDS
AACTGGAGACAATTGGTCGG+TGG 0.665926 3:-82682870 MsG0380016380.01.T01:CDS
CGGAACTGGAGACAATTGGT+CGG 0.666930 3:-82682873 MsG0380016380.01.T01:CDS
TGTCGAACCTGATTATACAT+CGG 0.676761 3:-82682893 MsG0380016380.01.T01:CDS
CGACGCAATAGAAGACGACA+CGG 0.728617 3:+82682795 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGATCAGTTCCATTCAAAT+GGG - Chr3:82682779-82682798 MsG0380016380.01.T01:CDS 35.0%
TGTCGAACCTGATTATACAT+CGG - Chr3:82683079-82683098 MsG0380016380.01.T01:CDS 35.0%
TTCAAATGGGAAATCAAACC+AGG - Chr3:82682792-82682811 MsG0380016380.01.T01:CDS 35.0%
! GGTTTGATTTCCCATTTGAA+TGG + Chr3:82682792-82682811 None:intergenic 35.0%
ACCTGATTATACATCGGAAC+TGG - Chr3:82683085-82683104 MsG0380016380.01.T01:CDS 40.0%
CAGGATCAGTTCCATTCAAA+TGG - Chr3:82682778-82682797 MsG0380016380.01.T01:CDS 40.0%
CATTTGAATGGAACTGATCC+TGG + Chr3:82682780-82682799 None:intergenic 40.0%
GATGATTCATTCAAGAAGCC+AGG - Chr3:82682759-82682778 MsG0380016380.01.T01:CDS 40.0%
TCCAGTTCCGATGTATAATC+AGG + Chr3:82683089-82683108 None:intergenic 40.0%
!!! ATTCCGGGTTTTGATGATGA+TGG + Chr3:82682836-82682855 None:intergenic 40.0%
!!! TTTTGATGATGATGGTGGTG+AGG + Chr3:82682828-82682847 None:intergenic 40.0%
ACATCGGAACTGGAGACAAT+TGG - Chr3:82683095-82683114 MsG0380016380.01.T01:CDS 45.0%
AGCTTGAATGAGGGAGATTC+CGG + Chr3:82682852-82682871 None:intergenic 45.0%
ATAGAAGACGACACGGTTGA+AGG + Chr3:82683173-82683192 None:intergenic 45.0%
CCACCATCATCATCAAAACC+CGG - Chr3:82682830-82682849 MsG0380016380.01.T01:CDS 45.0%
GGAGGTTTGAGCTTGAATGA+GGG + Chr3:82682861-82682880 None:intergenic 45.0%
TGGAGGTTTGAGCTTGAATG+AGG + Chr3:82682862-82682881 None:intergenic 45.0%
! ATCCACCGGAGATAACTTGT+AGG + Chr3:82682898-82682917 None:intergenic 45.0%
!! AACCCATTGGTGATGCTGAA+TGG - Chr3:82683207-82683226 MsG0380016380.01.T01:CDS 45.0%
!! ACCCATTGGTGATGCTGAAT+GGG - Chr3:82683208-82683227 MsG0380016380.01.T01:CDS 45.0%
!! ATGATGATGGTGGTGAGGAA+TGG + Chr3:82682823-82682842 None:intergenic 45.0%
!! TCTTCTCGCCTTTTCTGAAG+CGG - Chr3:82683024-82683043 MsG0380016380.01.T01:CDS 45.0%
!! TGATGATGGTGGTGAGGAAT+GGG + Chr3:82682822-82682841 None:intergenic 45.0%
AACCGACCTTAGGACTTGAC+CGG + Chr3:82682938-82682957 None:intergenic 50.0%
AACTGGAGACAATTGGTCGG+TGG - Chr3:82683102-82683121 MsG0380016380.01.T01:CDS 50.0%
AACTTGTAGGAGCCGAGTTG+CGG + Chr3:82682885-82682904 None:intergenic 50.0%
AGCCCATTCAGCATCACCAA+TGG + Chr3:82683213-82683232 None:intergenic 50.0%
CATCTCTCAGAACCGACCTT+AGG + Chr3:82682948-82682967 None:intergenic 50.0%
CGACGCAATAGAAGACGACA+CGG + Chr3:82683180-82683199 None:intergenic 50.0%
CGGAACTGGAGACAATTGGT+CGG - Chr3:82683099-82683118 MsG0380016380.01.T01:CDS 50.0%
CGGCTCCTACAAGTTATCTC+CGG - Chr3:82682890-82682909 MsG0380016380.01.T01:CDS 50.0%
CTCCTACAAGTTATCTCCGG+TGG - Chr3:82682893-82682912 MsG0380016380.01.T01:CDS 50.0%
GCCCATTCAGCATCACCAAT+GGG + Chr3:82683212-82683231 None:intergenic 50.0%
GCTTGAATGAGGGAGATTCC+GGG + Chr3:82682851-82682870 None:intergenic 50.0%
TTCCGGTCAAGTCCTAAGGT+CGG - Chr3:82682933-82682952 MsG0380016380.01.T01:CDS 50.0%
! CTGAGAGATGGCGGTTTGAA+CGG - Chr3:82682958-82682977 MsG0380016380.01.T01:CDS 50.0%
! GATGCTGAATGGGCTTGGTT+CGG - Chr3:82683218-82683237 MsG0380016380.01.T01:CDS 50.0%
!! CTAAGGTCGGTTCTGAGAGA+TGG - Chr3:82682946-82682965 MsG0380016380.01.T01:CDS 50.0%
!! GATGATGGTGGTGAGGAATG+GGG + Chr3:82682821-82682840 None:intergenic 50.0%
!! TCAGCATCACCAATGGGTTG+TGG + Chr3:82683206-82683225 None:intergenic 50.0%
!! TTGGTGATGCTGAATGGGCT+TGG - Chr3:82683213-82683232 MsG0380016380.01.T01:CDS 50.0%
!!! CCGGGTTTTGATGATGATGG+TGG + Chr3:82682833-82682852 None:intergenic 50.0%
ATTCCCGTACACGCTCCTTC+CGG - Chr3:82682916-82682935 MsG0380016380.01.T01:CDS 55.0%
CTAAGTCCTTGTCGCCGTTC+CGG - Chr3:82683144-82683163 MsG0380016380.01.T01:CDS 55.0%
CTCAAACCTCCACCGCAACT+CGG - Chr3:82682870-82682889 MsG0380016380.01.T01:CDS 55.0%
CTCCGTAGCCGCTTCAGAAA+AGG + Chr3:82683035-82683054 None:intergenic 55.0%
CTCCTTCCGGTCAAGTCCTA+AGG - Chr3:82682929-82682948 MsG0380016380.01.T01:CDS 55.0%
GACCTTAGGACTTGACCGGA+AGG + Chr3:82682934-82682953 None:intergenic 55.0%
! TTGACCGGAAGGAGCGTGTA+CGG + Chr3:82682923-82682942 None:intergenic 55.0%
!! AGGTCGGTTCTGAGAGATGG+CGG - Chr3:82682949-82682968 MsG0380016380.01.T01:CDS 55.0%
!!! CGCCTTTTCTGAAGCGGCTA+CGG - Chr3:82683030-82683049 MsG0380016380.01.T01:CDS 55.0%
CACTCCGGTTGCGCAAGAAG+GGG + Chr3:82682984-82683003 None:intergenic 60.0%
CCTGACGAAGTCACGCACTC+CGG + Chr3:82682999-82683018 None:intergenic 60.0%
GAGCGTGTACGGGAATCCAC+CGG + Chr3:82682912-82682931 None:intergenic 60.0%
GCACTCCGGTTGCGCAAGAA+GGG + Chr3:82682985-82683004 None:intergenic 60.0%
GCGTCGTCACCACAACCCAT+TGG - Chr3:82683194-82683213 MsG0380016380.01.T01:CDS 60.0%
TGGTGAGGAATGGGGAGTCC+TGG + Chr3:82682813-82682832 None:intergenic 60.0%
TTGTAGGAGCCGAGTTGCGG+TGG + Chr3:82682882-82682901 None:intergenic 60.0%
! TGACCGGAAGGAGCGTGTAC+GGG + Chr3:82682922-82682941 None:intergenic 60.0%
!! TTCTGAAGCGGCTACGGAGC+AGG - Chr3:82683036-82683055 MsG0380016380.01.T01:CDS 60.0%
ACGACACGGTTGAAGGCCGC+CGG + Chr3:82683166-82683185 None:intergenic 65.0%
ACGGCCCCTTCTTGCGCAAC+CGG - Chr3:82682977-82682996 MsG0380016380.01.T01:CDS 65.0%
ACGGTTGAAGGCCGCCGGAA+CGG + Chr3:82683161-82683180 None:intergenic 65.0%
AGTCCTTGTCGCCGTTCCGG+CGG - Chr3:82683147-82683166 MsG0380016380.01.T01:CDS 65.0%
CCGGAGTGCGTGACTTCGTC+AGG - Chr3:82682996-82683015 MsG0380016380.01.T01:CDS 65.0%
CGCACTCCGGTTGCGCAAGA+AGG + Chr3:82682986-82683005 None:intergenic 65.0%
TAGGAGCCGAGTTGCGGTGG+AGG + Chr3:82682879-82682898 None:intergenic 65.0%
AGGCCGCCGGAACGGCGACA+AGG + Chr3:82683153-82683172 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 82682744 82683250 82682744 ID=MsG0380016380.01;Name=MsG0380016380.01
Chr3 mRNA 82682744 82683250 82682744 ID=MsG0380016380.01.T01;Parent=MsG0380016380.01;Name=MsG0380016380.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|168
Chr3 exon 82682744 82683250 82682744 ID=MsG0380016380.01.T01:exon:33162;Parent=MsG0380016380.01.T01
Chr3 CDS 82682744 82683250 82682744 ID=MsG0380016380.01.T01:cds;Parent=MsG0380016380.01.T01
Gene Sequence

>MsG0380016380.01.T01

ATGGTTGTGGCGGAAGATGATTCATTCAAGAAGCCAGGATCAGTTCCATTCAAATGGGAAATCAAACCAGGACTCCCCATTCCTCACCACCATCATCATCAAAACCCGGAATCTCCCTCATTCAAGCTCAAACCTCCACCGCAACTCGGCTCCTACAAGTTATCTCCGGTGGATTCCCGTACACGCTCCTTCCGGTCAAGTCCTAAGGTCGGTTCTGAGAGATGGCGGTTTGAACGGCCCCTTCTTGCGCAACCGGAGTGCGTGACTTCGTCAGGTTGTTTCTTCTCGCCTTTTCTGAAGCGGCTACGGAGCAGGAAGAAGCTTGTGAAGAAAGTTGTCGAACCTGATTATACATCGGAACTGGAGACAATTGGTCGGTGGTCATTTTCGTCGTCTACTACTAAGTCCTTGTCGCCGTTCCGGCGGCCTTCAACCGTGTCGTCTTCTATTGCGTCGTCACCACAACCCATTGGTGATGCTGAATGGGCTTGGTTCGGTCTTTTCTGA

Protein sequence

>MsG0380016380.01.T01

MVVAEDDSFKKPGSVPFKWEIKPGLPIPHHHHHQNPESPSFKLKPPPQLGSYKLSPVDSRTRSFRSSPKVGSERWRFERPLLAQPECVTSSGCFFSPFLKRLRSRKKLVKKVVEPDYTSELETIGRWSFSSSTTKSLSPFRRPSTVSSSIASSPQPIGDAEWAWFGLF*