AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018423.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018423.01.T01 MTR_4g011530 67.089 79 20 5 17 91 366 442 3.04e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGGAGAAGGTGTTTGA+TGG 0.360591 4:+4735780 MsG0480018423.01.T01:CDS
AGAGGTTTGGTGTGCATGTT+TGG 0.362877 4:+4735735 MsG0480018423.01.T01:CDS
GGAGATGATCAGGCTGAGTT+TGG 0.390818 4:+4735801 MsG0480018423.01.T01:CDS
GAGTAAGAGGTTTGGTGCTA+AGG 0.439417 4:+4735622 MsG0480018423.01.T01:CDS
GAGAATGGTGAGAAGAAGAA+AGG 0.452968 4:+4735587 None:intergenic
AGAATGGTGAGAAGAAGAAA+GGG 0.468335 4:+4735588 None:intergenic
TTGGTAAGGAGAGAGTGTTA+TGG 0.478402 4:+4735754 MsG0480018423.01.T01:CDS
TTTAAGTGGAGTAAGAGGTT+TGG 0.480562 4:+4735614 MsG0480018423.01.T01:CDS
AGTAAGAGGTTTGGTGCTAA+GGG 0.490866 4:+4735623 MsG0480018423.01.T01:CDS
TTCATATCAGAAGTAGTAGC+AGG 0.495081 4:-4735834 None:intergenic
GGTGTTTGATGGAGATGATC+AGG 0.499983 4:+4735791 MsG0480018423.01.T01:CDS
AGAGATGTTGAGAAGAAGAA+AGG 0.502323 4:+4735696 MsG0480018423.01.T01:CDS
TTTGGTGTGCATGTTTGGTA+AGG 0.523361 4:+4735740 MsG0480018423.01.T01:CDS
GTATGCAATGCAAGTTCAGA+TGG 0.530977 4:+4735673 MsG0480018423.01.T01:CDS
GTTATGGAGAGAAGAGGAGA+AGG 0.531293 4:+4735770 MsG0480018423.01.T01:CDS
TGCTAAGGGAAGATGTTCAT+AGG 0.533231 4:+4735637 MsG0480018423.01.T01:CDS
GAGAGTGTTATGGAGAGAAG+AGG 0.565489 4:+4735764 MsG0480018423.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTACTTCTGATATGAAAAAT+AGG + Chr4:4735841-4735860 MsG0480018423.01.T01:CDS 25.0%
CTTCTGATATGAAAAATAGG+TGG + Chr4:4735844-4735863 MsG0480018423.01.T01:CDS 30.0%
!!! AGAAGAAAGGGTTTTTTAAG+TGG + Chr4:4735600-4735619 MsG0480018423.01.T01:CDS 30.0%
!!! AGAAGAAAGGTGTTTTTAAG+TGG + Chr4:4735709-4735728 MsG0480018423.01.T01:CDS 30.0%
AGAGATGTTGAGAAGAAGAA+AGG + Chr4:4735696-4735715 MsG0480018423.01.T01:CDS 35.0%
TTCATATCAGAAGTAGTAGC+AGG - Chr4:4735837-4735856 None:intergenic 35.0%
TTTAAGTGGAGTAAGAGGTT+TGG + Chr4:4735614-4735633 MsG0480018423.01.T01:CDS 35.0%
!! TTTTAAGTGGAGTAGAGGTT+TGG + Chr4:4735722-4735741 MsG0480018423.01.T01:CDS 35.0%
!!! GGTTTTTTAAGTGGAGTAAG+AGG + Chr4:4735609-4735628 MsG0480018423.01.T01:CDS 35.0%
GTATGCAATGCAAGTTCAGA+TGG + Chr4:4735673-4735692 MsG0480018423.01.T01:CDS 40.0%
TGCTAAGGGAAGATGTTCAT+AGG + Chr4:4735637-4735656 MsG0480018423.01.T01:CDS 40.0%
! TTTGGTGTGCATGTTTGGTA+AGG + Chr4:4735740-4735759 MsG0480018423.01.T01:CDS 40.0%
!! AGTAAGAGGTTTGGTGCTAA+GGG + Chr4:4735623-4735642 MsG0480018423.01.T01:CDS 40.0%
!! TTGGTAAGGAGAGAGTGTTA+TGG + Chr4:4735754-4735773 MsG0480018423.01.T01:CDS 40.0%
!!! GGTGTTTTTAAGTGGAGTAG+AGG + Chr4:4735717-4735736 MsG0480018423.01.T01:CDS 40.0%
GGTGTTTGATGGAGATGATC+AGG + Chr4:4735791-4735810 MsG0480018423.01.T01:CDS 45.0%
GTTATGGAGAGAAGAGGAGA+AGG + Chr4:4735770-4735789 MsG0480018423.01.T01:CDS 45.0%
!! AGAGGTTTGGTGTGCATGTT+TGG + Chr4:4735735-4735754 MsG0480018423.01.T01:CDS 45.0%
!! GAAGAGGAGAAGGTGTTTGA+TGG + Chr4:4735780-4735799 MsG0480018423.01.T01:CDS 45.0%
!! GAGAGTGTTATGGAGAGAAG+AGG + Chr4:4735764-4735783 MsG0480018423.01.T01:CDS 45.0%
!! GAGTAAGAGGTTTGGTGCTA+AGG + Chr4:4735622-4735641 MsG0480018423.01.T01:CDS 45.0%
GGAGATGATCAGGCTGAGTT+TGG + Chr4:4735801-4735820 MsG0480018423.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 4735591 4735869 4735591 ID=MsG0480018423.01;Name=MsG0480018423.01
Chr4 mRNA 4735591 4735869 4735591 ID=MsG0480018423.01.T01;Parent=MsG0480018423.01;Name=MsG0480018423.01.T01;_AED=0.42;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|92
Chr4 exon 4735591 4735869 4735591 ID=MsG0480018423.01.T01:exon:1254;Parent=MsG0480018423.01.T01
Chr4 CDS 4735591 4735869 4735591 ID=MsG0480018423.01.T01:cds;Parent=MsG0480018423.01.T01
Gene Sequence

>MsG0480018423.01.T01

ATGGTGAGAAGAAGAAAGGGTTTTTTAAGTGGAGTAAGAGGTTTGGTGCTAAGGGAAGATGTTCATAGGCTGCAAAGTCAGTGTATGCAATGCAAGTTCAGATGGAGAGATGTTGAGAAGAAGAAAGGTGTTTTTAAGTGGAGTAGAGGTTTGGTGTGCATGTTTGGTAAGGAGAGAGTGTTATGGAGAGAAGAGGAGAAGGTGTTTGATGGAGATGATCAGGCTGAGTTTGGAAGACAGACACCTGCTACTACTTCTGATATGAAAAATAGGTGGTGA

Protein sequence

>MsG0480018423.01.T01

MVRRRKGFLSGVRGLVLREDVHRLQSQCMQCKFRWRDVEKKKGVFKWSRGLVCMFGKERVLWREEEKVFDGDDQAEFGRQTPATTSDMKNRW*