AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1177090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177090 MtrunA17_Chr8g0347101 96.765 371 12 0 1 371 1 371 0.0 733
Msa1177090 MtrunA17_Chr3g0126611 42.210 353 189 9 17 359 29 376 7.47e-85 262
Msa1177090 MtrunA17_Chr8g0388231 40.000 360 177 13 26 359 36 382 9.99e-61 201
Msa1177090 MtrunA17_Chr7g0275601 54.762 168 57 5 202 362 1 156 2.90e-51 168
Msa1177090 MtrunA17_Chr8g0388201 34.688 369 200 11 26 362 36 395 2.43e-50 173
Msa1177090 MtrunA17_Chr5g0396601 33.613 357 202 13 30 359 47 395 1.04e-47 166
Msa1177090 MtrunA17_Chr5g0396611 33.062 369 207 16 21 359 27 385 3.52e-40 146
Msa1177090 MtrunA17_Chr3g0081611 46.000 150 66 2 227 366 16 160 1.75e-38 135
Msa1177090 MtrunA17_Chr5g0396591 34.884 258 159 8 108 359 31 285 2.72e-35 130
Msa1177090 MtrunA17_Chr1g0160731 36.214 243 121 12 1 225 8 234 1.65e-27 114
Msa1177090 MtrunA17_Chr1g0160671 33.054 239 128 11 3 222 10 235 3.52e-25 103
Msa1177090 MtrunA17_Chr1g0160581 33.197 244 125 12 1 217 1 233 3.13e-24 104
Msa1177090 MtrunA17_Chr1g0160771 32.661 248 123 11 26 255 40 261 5.50e-23 100
Msa1177090 MtrunA17_Chr7g0248831 40.217 92 55 0 275 366 69 160 4.20e-20 86.7
Msa1177090 MtrunA17_Chr8g0348151 41.237 97 53 1 269 365 73 165 2.09e-19 85.5
Msa1177090 MtrunA17_Chr1g0177041 39.560 91 53 2 278 366 65 155 3.78e-18 81.3
Msa1177090 MtrunA17_Chr1g0158651 29.752 242 133 11 26 255 53 269 1.36e-17 84.3
Msa1177090 MtrunA17_Chr1g0197031 28.800 125 84 3 243 364 30 152 7.62e-17 77.4
Msa1177090 MtrunA17_Chr1g0158771 36.364 165 89 6 33 189 45 201 2.88e-16 80.1
Msa1177090 MtrunA17_Chr4g0037001 27.917 240 142 9 32 253 44 270 3.10e-16 80.1
Msa1177090 MtrunA17_Chr1g0158311 26.616 263 162 11 26 277 33 275 5.99e-16 79.3
Msa1177090 MtrunA17_Chr7g0248841 36.735 98 59 2 272 366 59 156 1.06e-15 73.9
Msa1177090 MtrunA17_Chr7g0273051 38.400 125 74 2 242 363 45 169 1.86e-15 77.0
Msa1177090 MtrunA17_Chr5g0409211 34.074 135 81 4 233 364 15 144 5.57e-15 72.4
Msa1177090 MtrunA17_Chr1g0197021 42.857 70 37 1 298 364 80 149 8.75e-15 71.6
Msa1177090 MtrunA17_Chr2g0300331 31.724 145 79 4 235 370 92 225 1.10e-14 73.2
Msa1177090 MtrunA17_Chr1g0158531 28.879 232 130 12 33 251 37 246 1.65e-14 75.1
Msa1177090 MtrunA17_Chr1g0157971 30.556 216 123 9 17 216 24 228 1.68e-14 74.7
Msa1177090 MtrunA17_Chr5g0409201 51.923 52 25 0 312 363 91 142 4.23e-14 69.3
Msa1177090 MtrunA17_Chr3g0113811 32.847 137 78 5 226 358 24 150 5.10e-14 69.7
Msa1177090 MtrunA17_Chr5g0426881 41.176 85 49 1 286 369 114 198 5.58e-14 72.8
Msa1177090 MtrunA17_Chr5g0399521 31.098 164 97 7 33 189 29 183 1.18e-13 70.1
Msa1177090 MtrunA17_Chr1g0158621 41.121 107 51 5 26 121 36 141 1.59e-13 67.8
Msa1177090 MtrunA17_Chr1g0191601 34.752 141 59 4 242 363 50 176 1.59e-13 71.2
Msa1177090 MtrunA17_Chr6g0486651 28.758 153 92 5 206 357 26 162 3.86e-13 70.1
Msa1177090 MtrunA17_Chr4g0027761 45.070 71 34 1 286 356 100 165 9.46e-13 66.6
Msa1177090 MtrunA17_Chr2g0295571 42.105 76 42 2 281 356 88 161 1.31e-12 67.8
Msa1177090 MtrunA17_Chr3g0126931 40.964 83 37 2 280 357 95 170 1.53e-12 68.2
Msa1177090 MtrunA17_Chr7g0274711 41.096 73 42 1 286 357 67 139 1.75e-12 65.5
Msa1177090 MtrunA17_Chr7g0246761 55.319 47 21 0 310 356 86 132 1.77e-12 65.5
Msa1177090 MtrunA17_Chr8g0336881 30.000 140 93 2 228 367 24 158 1.81e-12 67.4
Msa1177090 MtrunA17_Chr7g0274741 39.474 76 45 1 283 357 67 142 2.06e-12 65.1
Msa1177090 MtrunA17_Chr5g0426781 34.351 131 64 5 243 356 71 196 2.21e-12 67.8
Msa1177090 MtrunA17_Chr3g0133001 39.726 73 40 2 285 356 129 198 3.12e-12 67.4
Msa1177090 MtrunA17_Chr8g0383921 35.526 76 48 1 282 357 107 181 3.19e-12 65.5
Msa1177090 MtrunA17_Chr1g0184631 41.096 73 42 1 286 357 68 140 3.47e-12 64.7
Msa1177090 MtrunA17_Chr1g0198201 49.020 51 26 0 308 358 82 132 4.92e-12 65.1
Msa1177090 MtrunA17_Chr4g0050041 38.095 84 48 2 286 368 126 206 6.47e-12 66.2
Msa1177090 MtrunA17_Chr8g0350741 62.791 43 16 0 314 356 89 131 6.51e-12 63.5
Msa1177090 MtrunA17_Chr5g0426871 37.975 79 48 1 286 363 108 186 8.35e-12 66.2
Msa1177090 MtrunA17_Chr8g0383871 36.111 72 45 1 286 357 112 182 8.41e-12 64.3
Msa1177090 MtrunA17_Chr8g0383941 28.696 115 80 2 243 357 65 177 1.20e-11 63.9
Msa1177090 MtrunA17_Chr1g0186661 34.188 117 71 2 247 358 60 175 1.50e-11 65.1
Msa1177090 MtrunA17_Chr3g0120011 40.845 71 39 1 292 362 100 167 1.55e-11 63.5
Msa1177090 MtrunA17_Chr8g0383861 36.111 72 45 1 286 357 113 183 1.85e-11 63.5
Msa1177090 MtrunA17_Chr8g0381731 39.726 73 40 2 285 356 147 216 2.16e-11 64.3
Msa1177090 MtrunA17_Chr8g0383881 36.111 72 45 1 286 357 103 173 2.29e-11 62.8
Msa1177090 MtrunA17_Chr3g0132491 36.145 83 52 1 286 367 107 189 2.82e-11 64.3
Msa1177090 MtrunA17_Chr3g0118071 34.722 72 47 0 286 357 76 147 3.06e-11 62.8
Msa1177090 MtrunA17_Chr3g0129671 29.032 124 83 2 233 356 60 178 3.21e-11 62.8
Msa1177090 MtrunA17_Chr7g0274731 52.273 44 21 0 313 356 113 156 3.34e-11 62.0
Msa1177090 MtrunA17_Chr8g0345381 30.435 92 58 1 281 366 14 105 3.79e-11 60.8
Msa1177090 MtrunA17_Chr8g0364881 39.189 74 43 1 285 358 67 138 3.90e-11 63.5
Msa1177090 MtrunA17_Chr1g0184171 38.824 85 43 3 286 366 103 182 4.85e-11 63.5
Msa1177090 MtrunA17_Chr8g0369571 28.477 151 90 5 217 364 5 140 5.04e-11 61.2
Msa1177090 MtrunA17_Chr5g0413841 39.189 74 43 1 285 358 115 186 5.51e-11 63.5
Msa1177090 MtrunA17_Chr7g0274721 51.111 45 22 0 313 357 92 136 5.60e-11 60.8
Msa1177090 MtrunA17_Chr8g0343571 37.805 82 46 2 281 357 47 128 5.94e-11 60.8
Msa1177090 MtrunA17_Chr2g0325141 27.407 135 85 4 238 361 6 138 6.36e-11 60.1
Msa1177090 MtrunA17_Chr1g0203541 51.163 43 21 0 314 356 136 178 6.71e-11 61.2
Msa1177090 MtrunA17_Chr3g0118061 34.722 72 47 0 286 357 76 147 8.12e-11 61.2
Msa1177090 MtrunA17_Chr3g0123631 41.818 55 32 0 308 362 69 123 8.81e-11 60.8
Msa1177090 MtrunA17_Chr2g0317671 29.825 114 62 3 262 357 89 202 9.56e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177090 AT2G46495.1 46.742 353 171 9 19 362 24 368 6.96e-95 288
Msa1177090 AT2G25410.1 43.988 341 186 5 26 361 34 374 9.21e-91 278
Msa1177090 AT2G46495.2 45.042 353 149 10 19 362 24 340 4.94e-86 264
Msa1177090 AT5G53110.1 39.489 352 188 10 24 359 33 375 3.03e-76 240
Msa1177090 AT2G46494.1 42.249 329 179 6 36 360 41 362 1.15e-75 238
Msa1177090 AT1G28040.1 42.857 301 157 9 68 358 2 297 2.51e-63 204
Msa1177090 AT5G36001.1 37.571 354 156 10 21 371 30 321 4.64e-60 197
Msa1177090 AT5G07040.1 47.586 145 73 2 226 370 9 150 1.28e-38 135
Msa1177090 AT5G36001.2 40.976 205 100 7 21 222 30 216 3.79e-32 120
Msa1177090 AT2G46493.1 34.597 211 107 5 153 360 2 184 1.13e-31 118
Msa1177090 AT2G35910.1 52.439 82 35 2 286 366 121 199 4.90e-23 96.3
Msa1177090 AT3G61550.1 43.299 97 46 1 283 370 96 192 8.40e-20 87.4
Msa1177090 AT2G46160.1 44.828 87 42 1 283 363 101 187 3.04e-19 85.9
Msa1177090 AT2G46160.2 44.828 87 42 1 283 363 101 187 3.04e-19 85.9
Msa1177090 AT5G06490.1 41.176 85 45 1 284 363 94 178 1.00e-16 78.2
Msa1177090 AT4G09100.1 42.857 77 43 1 286 361 56 132 1.93e-16 75.9
Msa1177090 AT5G27420.1 41.489 94 51 2 281 370 90 183 3.25e-14 73.6
Msa1177090 AT1G35330.1 35.075 134 74 4 241 363 47 178 3.90e-14 73.2
Msa1177090 AT4G09120.1 34.559 136 70 4 233 363 51 172 4.42e-14 73.2
Msa1177090 AT2G34990.1 30.435 138 93 3 220 356 3 138 4.90e-14 72.4
Msa1177090 AT2G34990.2 30.435 138 93 3 220 356 25 160 5.38e-14 72.8
Msa1177090 AT3G05200.1 34.375 160 91 5 205 356 17 170 7.44e-14 72.8
Msa1177090 AT4G09130.1 39.216 102 51 3 280 371 85 185 1.01e-13 72.0
Msa1177090 AT1G04360.1 35.570 149 83 5 221 358 32 178 3.12e-13 70.9
Msa1177090 AT4G17905.2 30.714 140 80 2 230 356 88 223 5.98e-13 69.7
Msa1177090 AT4G09110.1 40.506 79 46 1 286 363 94 172 7.60e-13 68.9
Msa1177090 AT2G35000.1 31.496 127 83 2 234 356 51 177 8.65e-13 69.3
Msa1177090 AT4G17905.1 30.714 140 80 2 230 356 62 197 8.76e-13 68.9
Msa1177090 AT1G22500.1 40.000 90 53 1 282 370 85 174 1.11e-12 68.9
Msa1177090 AT4G40070.1 44.595 74 40 1 285 357 94 167 1.44e-12 68.2
Msa1177090 AT1G80400.1 45.455 66 34 2 298 362 338 402 1.66e-12 68.6
Msa1177090 AT4G10160.1 40.964 83 46 1 281 363 67 146 1.67e-12 66.6
Msa1177090 AT5G05810.1 39.506 81 45 2 285 364 119 196 2.41e-12 68.2
Msa1177090 AT4G28890.1 40.541 74 40 2 285 357 96 166 3.69e-12 67.8
Msa1177090 AT5G66070.5 33.333 108 62 3 260 357 112 219 4.04e-12 65.5
Msa1177090 AT5G66070.1 33.333 108 62 3 260 357 112 219 4.04e-12 65.5
Msa1177090 AT2G42350.1 40.260 77 43 1 286 362 75 148 4.22e-12 65.5
Msa1177090 AT1G72200.1 41.772 79 45 1 286 363 115 193 4.29e-12 67.4
Msa1177090 AT1G23980.1 39.189 74 43 1 285 358 117 188 4.70e-12 67.0
Msa1177090 AT1G49220.1 36.275 102 58 3 262 357 78 178 5.53e-12 65.9
Msa1177090 AT1G49200.1 34.091 88 57 1 270 357 93 179 6.59e-12 65.1
Msa1177090 AT4G35840.1 31.667 120 75 2 245 357 115 234 7.63e-12 65.1
Msa1177090 AT3G60220.1 36.538 104 55 3 267 370 76 168 7.84e-12 66.2
Msa1177090 AT4G10150.1 39.759 83 47 1 281 363 81 160 8.39e-12 65.1
Msa1177090 AT1G33480.1 40.244 82 46 1 281 362 70 148 9.30e-12 65.1
Msa1177090 AT5G66070.2 48.980 49 25 0 309 357 195 243 1.16e-11 64.7
Msa1177090 AT2G27940.1 33.663 101 54 3 264 357 89 183 1.30e-11 64.3
Msa1177090 AT1G14200.1 41.176 68 36 2 289 356 87 150 1.50e-11 63.2
Msa1177090 AT5G43420.1 31.788 151 74 6 231 356 34 180 1.52e-11 65.5
Msa1177090 AT2G17730.1 31.092 119 75 2 245 356 120 238 1.89e-11 63.9
Msa1177090 AT1G76410.1 31.967 122 72 3 243 357 30 147 2.06e-11 62.8
Msa1177090 AT2G20030.1 29.060 117 77 3 244 357 54 167 2.11e-11 65.1
Msa1177090 AT1G49230.1 29.412 119 80 2 242 357 57 174 2.28e-11 63.5
Msa1177090 AT5G57750.1 34.615 78 42 2 285 358 94 166 2.39e-11 63.2
Msa1177090 AT3G48030.2 30.137 146 83 5 230 362 38 177 2.99e-11 63.9
Msa1177090 AT5G17600.1 40.244 82 45 2 276 356 106 184 3.73e-11 64.3
Msa1177090 AT1G49210.1 36.111 72 45 1 286 357 108 178 3.76e-11 62.8
Msa1177090 AT3G18773.1 29.752 121 78 3 243 357 54 173 6.68e-11 62.0
Msa1177090 AT3G48030.1 30.137 146 83 5 230 362 116 255 6.82e-11 63.5
Msa1177090 AT3G16720.1 50.000 44 22 0 314 357 119 162 7.28e-11 63.2
Msa1177090 AT2G35420.1 39.024 82 45 2 275 356 69 145 8.63e-11 62.4
Msa1177090 AT3G10910.1 43.333 60 31 1 314 370 114 173 8.82e-11 60.8

Find 70 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGCCACATAAAGAAAA+AGG 0.259554 8_1:+9423732 None:intergenic
AGTCGTGACTACACCTTCTT+AGG 0.282841 8_1:-9423465 Msa1177090:CDS
TGGAAACAATGTTTGCATTC+AGG 0.289536 8_1:+9421277 None:intergenic
GGGGAGAGTCGACGTGTTCC+TGG 0.295592 8_1:-9421367 Msa1177090:CDS
ACTGAAAGGGGAAACGAATT+AGG 0.325250 8_1:+9423703 None:intergenic
TCTGATCATTGTTATTCTAA+TGG 0.337767 8_1:-9421542 Msa1177090:CDS
GGCTGTTCATCTCAGAATAT+AGG 0.339403 8_1:-9423444 Msa1177090:CDS
ATTGAGGCTTAGAAGACGTT+TGG 0.339581 8_1:+9423518 None:intergenic
CGACTAAAAGAAGCAATAAA+AGG 0.352176 8_1:+9423483 None:intergenic
CCTGCAATTACTGTGGCTTC+AGG 0.355180 8_1:+9421433 None:intergenic
AGCTTTGTCAATTCTCTGTC+TGG 0.363370 8_1:-9423354 Msa1177090:CDS
AACCGTTCTGGTCTTGCAAT+TGG 0.365731 8_1:+9423303 None:intergenic
CTTATGAGAAAGTAATTCTT+GGG 0.370232 8_1:-9421387 Msa1177090:CDS
GCAGGAGAGTTACGACAAAC+AGG 0.377091 8_1:+9421211 None:intergenic
TCTTATGAGAAAGTAATTCT+TGG 0.381733 8_1:-9421388 Msa1177090:CDS
TATCCTTATTGTGGCTATCC+TGG 0.384579 8_1:-9423669 Msa1177090:CDS
ATCCAACAACTTCCGTCGTT+TGG 0.392248 8_1:+9421343 None:intergenic
TACTTTCTCATAAGATTCAA+TGG 0.398432 8_1:+9421396 None:intergenic
CCTGAAGCCACAGTAATTGC+AGG 0.408761 8_1:-9421433 Msa1177090:CDS
ATTCGTTTCCCCTTTCAGTT+AGG 0.411401 8_1:-9423699 Msa1177090:CDS
TTACCAGAAGTGCTGCTTCT+CGG 0.415149 8_1:-9421483 Msa1177090:CDS
TGACCGAGAAGCAGCACTTC+TGG 0.415992 8_1:+9421480 None:intergenic
TTGAGGCTTAGAAGACGTTT+GGG 0.423213 8_1:+9423519 None:intergenic
AGAGTTACTAAGACAATCAA+TGG 0.428456 8_1:+9423407 None:intergenic
TCGTTTATGGCGTATGCGAC+AGG 0.432440 8_1:+9423759 None:intergenic
AAACCAGGATAGCCACAATA+AGG 0.435316 8_1:+9423666 None:intergenic
CTTTACTGTTGTATTCTGCC+AGG 0.440731 8_1:+9421317 None:intergenic
TTACTAGAGGCAACATTAAG+AGG 0.477123 8_1:+9421181 None:intergenic
ATTACTGTGGCTTCAGGTTG+CGG 0.477514 8_1:+9421439 None:intergenic
GCTGTTCATCTCAGAATATA+GGG 0.484289 8_1:-9423443 Msa1177090:CDS
CAACTTCCGTCGTTTGGTCC+AGG 0.484298 8_1:+9421349 None:intergenic
ACATAGAATTCTTCAGAGTA+AGG 0.498143 8_1:+9423600 None:intergenic
TATTGCTCACCTAACTGAAA+GGG 0.502186 8_1:+9423690 None:intergenic
ATCATAGCACTATGCGTCAC+TGG 0.507674 8_1:-9421571 Msa1177090:CDS
GACAAAGCTAACTGAAGGAA+TGG 0.510349 8_1:+9423368 None:intergenic
CGGAAGTTGTTGGATATGCC+TGG 0.513577 8_1:-9421335 Msa1177090:CDS
GACCAAACGACGGAAGTTGT+TGG 0.524157 8_1:-9421345 Msa1177090:CDS
ACGTTTGGGAAGACAATCAT+CGG 0.534356 8_1:+9423533 None:intergenic
AGAAGTAATGGACATCTTGA+AGG 0.536590 8_1:-9423802 None:intergenic
GTGCAGATCTTCAATTAACA+TGG 0.544292 8_1:-9423254 Msa1177090:CDS
ATAGGACATCTTTACCTGGT+TGG 0.546643 8_1:+9423139 None:intergenic
CTGAGATGAACAGCCTAAGA+AGG 0.553624 8_1:+9423452 None:intergenic
GATTCACAATAACTGCAGTC+AGG 0.554160 8_1:+9423225 None:intergenic
ACATTAAGAGGAGAAGGAGC+AGG 0.559825 8_1:+9421193 None:intergenic
TGTTTCTCTGATTACCAACC+AGG 0.560713 8_1:-9423153 Msa1177090:intron
ATATTGCTCACCTAACTGAA+AGG 0.561759 8_1:+9423689 None:intergenic
GGTGAGCAATATCCTTATTG+TGG 0.564899 8_1:-9423678 Msa1177090:CDS
TGCTTCTCGGTCAGCACCAG+CGG 0.567276 8_1:-9421470 Msa1177090:CDS
AGAATAACAATGATCAGAGC+TGG 0.567847 8_1:+9421547 None:intergenic
GAGGCAACATTAAGAGGAGA+AGG 0.567930 8_1:+9421187 None:intergenic
TTAATTGAAGATCTGCACTA+AGG 0.568451 8_1:+9423259 None:intergenic
CGTTTGGGAAGACAATCATC+GGG 0.574221 8_1:+9423534 None:intergenic
GGTTGCGGTGATATTGCCGC+TGG 0.576865 8_1:+9421454 None:intergenic
GTGTGCTACAATGATAGAAT+AGG 0.583305 8_1:-9421511 Msa1177090:CDS
GAGTTACTAAGACAATCAAT+GGG 0.593741 8_1:+9423408 None:intergenic
GTCTTGCAATTGGAACTGAA+AGG 0.594777 8_1:+9423313 None:intergenic
ATTGCTCACCTAACTGAAAG+GGG 0.601036 8_1:+9423691 None:intergenic
ATGCTGATTGCATCGATGAG+TGG 0.601507 8_1:-9421255 Msa1177090:CDS
GTACAATTTAAGTTGAAACC+AGG 0.606022 8_1:+9423651 None:intergenic
TGCAGATCTTCAATTAACAT+GGG 0.617198 8_1:-9423253 Msa1177090:CDS
TTTCTCATAAGATTCAATGG+TGG 0.628140 8_1:+9421399 None:intergenic
ATCTAAACCTGCAATTACTG+TGG 0.628907 8_1:+9421426 None:intergenic
GAATTGACAAAGCTAACTGA+AGG 0.634686 8_1:+9423363 None:intergenic
TTCCAATTGCAAGACCAGAA+CGG 0.642475 8_1:-9423305 Msa1177090:CDS
ACTCATCGATGCAATCAGCA+TGG 0.644887 8_1:+9421257 None:intergenic
AATCATTCAAGCATTACTAG+AGG 0.645917 8_1:+9421168 None:intergenic
TTATGAGAAAGTAATTCTTG+GGG 0.648958 8_1:-9421386 Msa1177090:CDS
CAGGAGAGTTACGACAAACA+GGG 0.678810 8_1:+9421212 None:intergenic
CGTGTTCCTGGACCAAACGA+CGG 0.694755 8_1:-9421355 Msa1177090:CDS
GAATCGCATCAGCTAATGTG+TGG 0.708361 8_1:-9423207 Msa1177090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCTTTTTTTTTTTGA+AGG + chr8_1:9422780-9422799 None:intergenic 10.0%
!! AAAAAAAGAAAAAAGATTTA+AGG - chr8_1:9422786-9422805 Msa1177090:intron 10.0%
!!! ATATCCAGAAATTAATTTTT+TGG - chr8_1:9421932-9421951 Msa1177090:intron 15.0%
!!! TTGCATTAATTTTTTTTTGA+AGG + chr8_1:9422277-9422296 None:intergenic 15.0%
!!! GATGTTATATTTTAGAAAAA+TGG - chr8_1:9422674-9422693 Msa1177090:intron 15.0%
!!! TTTTTTTTTCTTTTTTCTGA+AGG + chr8_1:9422901-9422920 None:intergenic 15.0%
!!! TTTTTTTGAAGAAATAACAA+AGG + chr8_1:9422974-9422993 None:intergenic 15.0%
!!! TTAATATTTTATTGCCTTTT+TGG + chr8_1:9423019-9423038 None:intergenic 15.0%
!! AAAAGGCAATAAAATATTAA+CGG - chr8_1:9423019-9423038 Msa1177090:intron 15.0%
!! TTTGGAAATGTAATAAAAAA+GGG + chr8_1:9423299-9423318 None:intergenic 15.0%
!! ATTTGGAAATGTAATAAAAA+AGG + chr8_1:9423300-9423319 None:intergenic 15.0%
!!! TTCAATATGCAAATTCTTTT+AGG - chr8_1:9421857-9421876 Msa1177090:intron 20.0%
!!! TATCCACAAATTAGTTTTTT+TGG - chr8_1:9421898-9421917 Msa1177090:intron 20.0%
!! TAACCAAAAAAACTAATTTG+TGG + chr8_1:9421904-9421923 None:intergenic 20.0%
!! CTAACCAAAAAATTAATTTC+TGG + chr8_1:9421939-9421958 None:intergenic 20.0%
!! AGGAATAACTAGAAAAATAT+TGG - chr8_1:9422101-9422120 Msa1177090:intron 20.0%
!! TGAACAAATTCATATAAAAG+TGG - chr8_1:9423083-9423102 Msa1177090:intron 20.0%
!! ATTACATTTCCAAATAAATG+AGG - chr8_1:9423305-9423324 Msa1177090:CDS 20.0%
! CAGGAAAGATTAAGTAAAAA+AGG + chr8_1:9421194-9421213 None:intergenic 25.0%
!!! TAGAATTGTTTTGCTATCAT+TGG + chr8_1:9421343-9421362 None:intergenic 25.0%
! AAGGAGAATTTGAAAAATTG+AGG + chr8_1:9421470-9421489 None:intergenic 25.0%
! ATTTGCATATTGAAGAACAT+AGG + chr8_1:9421851-9421870 None:intergenic 25.0%
!!! AAAAAACTACTTTCTGGTTA+GGG + chr8_1:9421967-9421986 None:intergenic 25.0%
!!! AAAAAAACTACTTTCTGGTT+AGG + chr8_1:9421968-9421987 None:intergenic 25.0%
!!! TAACCAGAAAGTAGTTTTTT+TGG - chr8_1:9421967-9421986 Msa1177090:intron 25.0%
! TAACCAAAAAAACTACTTTC+TGG + chr8_1:9421973-9421992 None:intergenic 25.0%
! GAGATAGAAATAAAACTAGT+TGG - chr8_1:9422059-9422078 Msa1177090:intron 25.0%
! ACTAGAAAAATATTGGTCAA+TGG - chr8_1:9422108-9422127 Msa1177090:intron 25.0%
!!! GATTTATTCCTATTGACTTA+AGG - chr8_1:9422179-9422198 Msa1177090:intron 25.0%
! AATAGAATCCTTAAGTCAAT+AGG + chr8_1:9422190-9422209 None:intergenic 25.0%
! ACAACATTATACTTCACTAA+TGG + chr8_1:9422538-9422557 None:intergenic 25.0%
!!! TTTTAAGTTGAAATTCATGC+AGG - chr8_1:9423165-9423184 Msa1177090:CDS 25.0%
!! TGGACATTTTTACATACTTT+TGG + chr8_1:9423239-9423258 None:intergenic 25.0%
! CAAATAAGACCTCATTTATT+TGG + chr8_1:9423317-9423336 None:intergenic 25.0%
!! TTATTTGTAGTTCCATTTTC+TGG - chr8_1:9423330-9423349 Msa1177090:CDS 25.0%
!! TCTGATCATTGTTATTCTAA+TGG - chr8_1:9423427-9423446 Msa1177090:CDS 25.0%
! TACTTTCTCATAAGATTCAA+TGG + chr8_1:9423576-9423595 None:intergenic 25.0%
! TCTTATGAGAAAGTAATTCT+TGG - chr8_1:9423581-9423600 Msa1177090:CDS 25.0%
! CTTATGAGAAAGTAATTCTT+GGG - chr8_1:9423582-9423601 Msa1177090:CDS 25.0%
! TTATGAGAAAGTAATTCTTG+GGG - chr8_1:9423583-9423602 Msa1177090:CDS 25.0%
TGTTGCCACATAAAGAAAAA+GGG + chr8_1:9421239-9421258 None:intergenic 30.0%
TTGTTGCCACATAAAGAAAA+AGG + chr8_1:9421240-9421259 None:intergenic 30.0%
!! GTACAATTTAAGTTGAAACC+AGG + chr8_1:9421321-9421340 None:intergenic 30.0%
ACATAGAATTCTTCAGAGTA+AGG + chr8_1:9421372-9421391 None:intergenic 30.0%
CGACTAAAAGAAGCAATAAA+AGG + chr8_1:9421489-9421508 None:intergenic 30.0%
GAGTTACTAAGACAATCAAT+GGG + chr8_1:9421564-9421583 None:intergenic 30.0%
AGAGTTACTAAGACAATCAA+TGG + chr8_1:9421565-9421584 None:intergenic 30.0%
TTAATTGAAGATCTGCACTA+AGG + chr8_1:9421713-9421732 None:intergenic 30.0%
TGCAGATCTTCAATTAACAT+GGG - chr8_1:9421716-9421735 Msa1177090:intron 30.0%
TAAGGATTCTATTTGCATGT+TGG - chr8_1:9422197-9422216 Msa1177090:intron 30.0%
! TTTGCATGTTGGCAATTTTT+CGG - chr8_1:9422208-9422227 Msa1177090:intron 30.0%
AACGTTGCACTCTTTAAATT+TGG + chr8_1:9422339-9422358 None:intergenic 30.0%
ATTATACTTCACTAATGGCA+CGG + chr8_1:9422533-9422552 None:intergenic 30.0%
GAAATTATTTGTGAGCAATG+AGG - chr8_1:9422842-9422861 Msa1177090:intron 30.0%
! CTTTTCATTTTCAATCGAAC+AGG + chr8_1:9422927-9422946 None:intergenic 30.0%
GAAATAACAAAGGAAGAGTT+GGG + chr8_1:9422964-9422983 None:intergenic 30.0%
AGAAATAACAAAGGAAGAGT+TGG + chr8_1:9422965-9422984 None:intergenic 30.0%
! TTCCTTTGTTATAGCCAAAA+AGG - chr8_1:9423002-9423021 Msa1177090:intron 30.0%
GAGGATAGGATATATAAGTT+GGG + chr8_1:9423277-9423296 None:intergenic 30.0%
TGTAATAAAAAAGGGAGGAT+AGG + chr8_1:9423291-9423310 None:intergenic 30.0%
GGAAATGTAATAAAAAAGGG+AGG + chr8_1:9423296-9423315 None:intergenic 30.0%
TGAAAATTGAAGCCAGAAAA+TGG + chr8_1:9423345-9423364 None:intergenic 30.0%
!!! ATTTTCTGGCTTCAATTTTC+AGG - chr8_1:9423344-9423363 Msa1177090:CDS 30.0%
TTTCTCATAAGATTCAATGG+TGG + chr8_1:9423573-9423592 None:intergenic 30.0%
AGAAAAAGGGCAATCGTTTA+TGG + chr8_1:9421226-9421245 None:intergenic 35.0%
! GATTGCCCTTTTTCTTTATG+TGG - chr8_1:9421231-9421250 Msa1177090:CDS 35.0%
TATTGCTCACCTAACTGAAA+GGG + chr8_1:9421282-9421301 None:intergenic 35.0%
ATATTGCTCACCTAACTGAA+AGG + chr8_1:9421283-9421302 None:intergenic 35.0%
GCTGTTCATCTCAGAATATA+GGG - chr8_1:9421526-9421545 Msa1177090:CDS 35.0%
GAATTGACAAAGCTAACTGA+AGG + chr8_1:9421609-9421628 None:intergenic 35.0%
! AGATTTTCTTGAAACCGTTC+TGG + chr8_1:9421681-9421700 None:intergenic 35.0%
GTGCAGATCTTCAATTAACA+TGG - chr8_1:9421715-9421734 Msa1177090:intron 35.0%
ATCAGTTGACTAAACGTGTT+TGG - chr8_1:9422011-9422030 Msa1177090:intron 35.0%
TTCGGTAAACATTTGCAAGT+TGG - chr8_1:9422226-9422245 Msa1177090:intron 35.0%
! GTGACTTATAATTTGAGACC+CGG - chr8_1:9422635-9422654 Msa1177090:intron 35.0%
ATCAAGTTCTTGATCTACTC+CGG + chr8_1:9422657-9422676 None:intergenic 35.0%
!!! TGCCTTTTTGGCTATAACAA+AGG + chr8_1:9423007-9423026 None:intergenic 35.0%
! TAGGATATATAAGTTGGGAG+TGG + chr8_1:9423272-9423291 None:intergenic 35.0%
GGAGGATAGGATATATAAGT+TGG + chr8_1:9423278-9423297 None:intergenic 35.0%
AGAATAACAATGATCAGAGC+TGG + chr8_1:9423425-9423444 None:intergenic 35.0%
GTGTGCTACAATGATAGAAT+AGG - chr8_1:9423458-9423477 Msa1177090:CDS 35.0%
ATCTAAACCTGCAATTACTG+TGG + chr8_1:9423546-9423565 None:intergenic 35.0%
TGGAAACAATGTTTGCATTC+AGG + chr8_1:9423695-9423714 None:intergenic 35.0%
TTACTAGAGGCAACATTAAG+AGG + chr8_1:9423791-9423810 None:intergenic 35.0%
ACTGAAAGGGGAAACGAATT+AGG + chr8_1:9421269-9421288 None:intergenic 40.0%
ATTCGTTTCCCCTTTCAGTT+AGG - chr8_1:9421270-9421289 Msa1177090:CDS 40.0%
ATTGCTCACCTAACTGAAAG+GGG + chr8_1:9421281-9421300 None:intergenic 40.0%
GGTGAGCAATATCCTTATTG+TGG - chr8_1:9421291-9421310 Msa1177090:CDS 40.0%
TATCCTTATTGTGGCTATCC+TGG - chr8_1:9421300-9421319 Msa1177090:CDS 40.0%
AAACCAGGATAGCCACAATA+AGG + chr8_1:9421306-9421325 None:intergenic 40.0%
ACGTTTGGGAAGACAATCAT+CGG + chr8_1:9421439-9421458 None:intergenic 40.0%
TTGAGGCTTAGAAGACGTTT+GGG + chr8_1:9421453-9421472 None:intergenic 40.0%
ATTGAGGCTTAGAAGACGTT+TGG + chr8_1:9421454-9421473 None:intergenic 40.0%
GGCTGTTCATCTCAGAATAT+AGG - chr8_1:9421525-9421544 Msa1177090:CDS 40.0%
GACAAAGCTAACTGAAGGAA+TGG + chr8_1:9421604-9421623 None:intergenic 40.0%
! AGCTTTGTCAATTCTCTGTC+TGG - chr8_1:9421615-9421634 Msa1177090:intron 40.0%
GTCTTGCAATTGGAACTGAA+AGG + chr8_1:9421659-9421678 None:intergenic 40.0%
TTCCAATTGCAAGACCAGAA+CGG - chr8_1:9421664-9421683 Msa1177090:intron 40.0%
GATTCACAATAACTGCAGTC+AGG + chr8_1:9421747-9421766 None:intergenic 40.0%
TGTTTCTCTGATTACCAACC+AGG - chr8_1:9421816-9421835 Msa1177090:intron 40.0%
ATAGGACATCTTTACCTGGT+TGG + chr8_1:9421833-9421852 None:intergenic 40.0%
GAACATAGGACATCTTTACC+TGG + chr8_1:9421837-9421856 None:intergenic 40.0%
TCCTTCTAACCTAAGCATGT+AGG + chr8_1:9422085-9422104 None:intergenic 40.0%
CATGTTTATCTCAATCGCTG+AGG + chr8_1:9422139-9422158 None:intergenic 40.0%
CTCAGCGATTGAGATAAACA+TGG - chr8_1:9422137-9422156 Msa1177090:intron 40.0%
GGCTTACGATATGTACGAAT+GGG + chr8_1:9422318-9422337 None:intergenic 40.0%
TGGCTTACGATATGTACGAA+TGG + chr8_1:9422319-9422338 None:intergenic 40.0%
TCAAGTTCTTGATCTACTCC+GGG + chr8_1:9422656-9422675 None:intergenic 40.0%
CTTTACTGTTGTATTCTGCC+AGG + chr8_1:9423655-9423674 None:intergenic 40.0%
CGTTTGGGAAGACAATCATC+GGG + chr8_1:9421438-9421457 None:intergenic 45.0%
AGTCGTGACTACACCTTCTT+AGG - chr8_1:9421504-9421523 Msa1177090:CDS 45.0%
CTGAGATGAACAGCCTAAGA+AGG + chr8_1:9421520-9421539 None:intergenic 45.0%
!! AACCGTTCTGGTCTTGCAAT+TGG + chr8_1:9421669-9421688 None:intergenic 45.0%
GAATCGCATCAGCTAATGTG+TGG - chr8_1:9421762-9421781 Msa1177090:intron 45.0%
! ACTAGTTGGCCTACATGCTT+AGG - chr8_1:9422073-9422092 Msa1177090:intron 45.0%
GCCTACATGCTTAGGTTAGA+AGG - chr8_1:9422081-9422100 Msa1177090:intron 45.0%
GTCTGGTAAGCAATTGTCTC+TGG + chr8_1:9422384-9422403 None:intergenic 45.0%
GCTTACCAGACTAACACTCT+CGG - chr8_1:9422393-9422412 Msa1177090:intron 45.0%
!! TGATACCGAGAGTGTTAGTC+TGG + chr8_1:9422401-9422420 None:intergenic 45.0%
!!! TTTTCAGGTACATCGCGACA+AGG - chr8_1:9423359-9423378 Msa1177090:CDS 45.0%
ATCATAGCACTATGCGTCAC+TGG - chr8_1:9423398-9423417 Msa1177090:CDS 45.0%
!! TTACCAGAAGTGCTGCTTCT+CGG - chr8_1:9423486-9423505 Msa1177090:CDS 45.0%
!! ATTACTGTGGCTTCAGGTTG+CGG + chr8_1:9423533-9423552 None:intergenic 45.0%
ATCCAACAACTTCCGTCGTT+TGG + chr8_1:9423629-9423648 None:intergenic 45.0%
ACTCATCGATGCAATCAGCA+TGG + chr8_1:9423715-9423734 None:intergenic 45.0%
! ATGCTGATTGCATCGATGAG+TGG - chr8_1:9423714-9423733 Msa1177090:CDS 45.0%
CAGGAGAGTTACGACAAACA+GGG + chr8_1:9423760-9423779 None:intergenic 45.0%
ACATTAAGAGGAGAAGGAGC+AGG + chr8_1:9423779-9423798 None:intergenic 45.0%
GAGGCAACATTAAGAGGAGA+AGG + chr8_1:9423785-9423804 None:intergenic 45.0%
!! TAAAATAATTAAAATATTCT+AGG - chr8_1:9422706-9422725 Msa1177090:intron 5.0%
!! AAAATAATTAAAATATTCTA+GGG - chr8_1:9422707-9422726 Msa1177090:intron 5.0%
TCGTTTATGGCGTATGCGAC+AGG + chr8_1:9421213-9421232 None:intergenic 50.0%
CCTGCAATTACTGTGGCTTC+AGG + chr8_1:9423539-9423558 None:intergenic 50.0%
CCTGAAGCCACAGTAATTGC+AGG - chr8_1:9423536-9423555 Msa1177090:CDS 50.0%
GACCAAACGACGGAAGTTGT+TGG - chr8_1:9423624-9423643 Msa1177090:CDS 50.0%
CGGAAGTTGTTGGATATGCC+TGG - chr8_1:9423634-9423653 Msa1177090:CDS 50.0%
GCAGGAGAGTTACGACAAAC+AGG + chr8_1:9423761-9423780 None:intergenic 50.0%
TTGGGAGTGGCAGCAACTCA+TGG + chr8_1:9423259-9423278 None:intergenic 55.0%
TGACCGAGAAGCAGCACTTC+TGG + chr8_1:9423492-9423511 None:intergenic 55.0%
CGTGTTCCTGGACCAAACGA+CGG - chr8_1:9423614-9423633 Msa1177090:CDS 55.0%
CAACTTCCGTCGTTTGGTCC+AGG + chr8_1:9423623-9423642 None:intergenic 55.0%
TGCTTCTCGGTCAGCACCAG+CGG - chr8_1:9423499-9423518 Msa1177090:CDS 60.0%
GGTTGCGGTGATATTGCCGC+TGG + chr8_1:9423518-9423537 None:intergenic 60.0%
GGGGAGAGTCGACGTGTTCC+TGG - chr8_1:9423602-9423621 Msa1177090:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 9421174 9423817 9421174 ID=Msa1177090;Name=Msa1177090
chr8_1 mRNA 9421174 9423817 9421174 ID=Msa1177090-mRNA-1;Parent=Msa1177090;Name=Msa1177090-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|371
chr8_1 exon 9423154 9423817 9423154 ID=Msa1177090-mRNA-1:exon:3202;Parent=Msa1177090-mRNA-1
chr8_1 exon 9421174 9421625 9421174 ID=Msa1177090-mRNA-1:exon:3201;Parent=Msa1177090-mRNA-1
chr8_1 CDS 9423154 9423817 9423154 ID=Msa1177090-mRNA-1:cds;Parent=Msa1177090-mRNA-1
chr8_1 CDS 9421174 9421625 9421174 ID=Msa1177090-mRNA-1:cds;Parent=Msa1177090-mRNA-1
Gene Sequence

>Msa1177090

ATGGACATCTTGAAGGTCCTTTTTTACTTAATCTTTCCTGTCGCATACGCCATAAACGATTGCCCTTTTTCTTTATGTGGCAACAATAGTTTCCTAATTCGTTTCCCCTTTCAGTTAGGTGAGCAATATCCTTATTGTGGCTATCCTGGTTTCAACTTAAATTGTACCAATGATAGCAAAACAATTCTAAAACTTCCTTACTCTGAAGAATTCTATGTACGCAGCATAAACTATCTCACGCAACAGATACTACTCTATGATCCCGATGATTGTCTTCCCAAACGTCTTCTAAGCCTCAATTTTTCAAATTCTCCTTTTATTGCTTCTTTTAGTCGTGACTACACCTTCTTAGGCTGTTCATCTCAGAATATAGGGTCACAATTCATTCCCATTGATTGTCTTAGTAACTCTACATATTTTGTATCAGCCATTCCTTCAGTTAGCTTTGTCAATTCTCTGTCTGGTTGCTCTGTTATAAAAAACCTTTCAGTTCCAATTGCAAGACCAGAACGGTTTCAAGAAAATCTAAGAGATGACCTTAGTGCAGATCTTCAATTAACATGGGATAAACCTGACTGCAGTTATTGTGAATCGCATCAGCTAATGTGTGGATTTGAAAGCATTAACAGCAATCAAGTTGTCTGTTTCTCTGATTACCAACCAGGTACATCGCGACAAGGACTTAAAATTTTCAGAATCATAGCACTATGCGTCACTGGACCAGCTCTGATCATTGTTATTCTAATGGCAAGTTGTGTGTGCTACAATGATAGAATAGGCAACATTACCAGAAGTGCTGCTTCTCGGTCAGCACCAGCGGCAATATCACCGCAACCTGAAGCCACAGTAATTGCAGGTTTAGATGAATCCACCATTGAATCTTATGAGAAAGTAATTCTTGGGGAGAGTCGACGTGTTCCTGGACCAAACGACGGAAGTTGTTGGATATGCCTGGCAGAATACAACAGTAAAGAGACAGTAAGATGCATTCCTGAATGCAAACATTGTTTCCATGCTGATTGCATCGATGAGTGGCTTCGTATGAATGTTACATGCCCTGTTTGTCGTAACTCTCCTGCTCCTTCTCCTCTTAATGTTGCCTCTAGTAATGCTTGA
Protein sequence

>Msa1177090

MDILKVLFYLIFPVAYAINDCPFSLCGNNSFLIRFPFQLGEQYPYCGYPGFNLNCTNDSKTILKLPYSEEFYVRSINYLTQQILLYDPDDCLPKRLLSLNFSNSPFIASFSRDYTFLGCSSQNIGSQFIPIDCLSNSTYFVSAIPSVSFVNSLSGCSVIKNLSVPIARPERFQENLRDDLSADLQLTWDKPDCSYCESHQLMCGFESINSNQVVCFSDYQPGTSRQGLKIFRIIALCVTGPALIIVILMASCVCYNDRIGNITRSAASRSAPAAISPQPEATVIAGLDESTIESYEKVILGESRRVPGPNDGSCWICLAEYNSKETVRCIPECKHCFHADCIDEWLRMNVTCPVCRNSPAPSPLNVASSNA*