AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1095620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1095620 MtrunA17_Chr7g0223181 100.000 157 0 0 1 157 492 648 2.87e-106 317
Msa1095620 MtrunA17_Chr7g0262821 84.177 158 24 1 1 157 492 649 3.88e-86 265
Msa1095620 MtrunA17_Chr7g0223291 81.366 161 23 3 1 157 492 649 9.84e-82 253
Msa1095620 MtrunA17_Chr4g0031301 82.390 159 25 2 1 157 492 649 3.87e-77 241
Msa1095620 MtrunA17_Chr4g0031321 81.250 160 25 2 1 157 492 649 2.40e-76 239
Msa1095620 MtrunA17_Chr4g0073741 73.006 163 38 1 1 157 491 653 3.00e-68 218
Msa1095620 MtrunA17_Chr6g0462601 56.800 125 54 0 1 125 491 615 4.01e-39 139
Msa1095620 MtrunA17_Chr6g0462711 52.800 125 59 0 1 125 498 622 3.12e-38 137
Msa1095620 MtrunA17_Chr8g0388671 55.469 128 56 1 1 127 518 645 1.62e-35 129
Msa1095620 MtrunA17_Chr8g0388761 56.250 128 55 1 1 127 518 645 1.81e-35 129
Msa1095620 MtrunA17_Chr6g0462641 47.101 138 73 0 1 138 496 633 4.06e-35 128
Msa1095620 MtrunA17_Chr6g0462771 47.297 148 73 1 1 148 496 638 5.22e-35 128
Msa1095620 MtrunA17_Chr6g0462681 49.194 124 63 0 1 124 491 614 7.66e-34 125
Msa1095620 MtrunA17_Chr7g0223631 49.219 128 65 0 1 128 477 604 2.27e-33 123
Msa1095620 MtrunA17_Chr6g0462611 47.581 124 65 0 1 124 439 562 9.41e-32 119
Msa1095620 MtrunA17_Chr7g0223681 46.094 128 69 0 1 128 402 529 1.01e-30 115
Msa1095620 MtrunA17_Chr2g0284681 52.586 116 41 1 1 116 497 598 3.08e-30 114
Msa1095620 MtrunA17_Chr7g0223731 49.138 116 59 0 1 116 491 606 4.58e-30 114
Msa1095620 MtrunA17_Chr7g0223711 48.438 128 66 0 1 128 492 619 1.36e-29 112
Msa1095620 MtrunA17_Chr7g0223031 49.153 118 60 0 1 118 491 608 1.86e-29 112
Msa1095620 MtrunA17_Chr8g0350731 46.875 128 68 0 1 128 519 646 8.91e-29 110
Msa1095620 MtrunA17_Chr2g0298861 48.000 125 64 1 1 124 504 628 9.12e-29 110
Msa1095620 MtrunA17_Chr7g0223541 46.094 128 69 0 1 128 449 576 1.23e-28 110
Msa1095620 MtrunA17_Chr7g0223701 48.718 117 60 0 1 117 500 616 3.41e-28 108
Msa1095620 MtrunA17_Chr8g0390821 44.800 125 69 0 1 125 530 654 6.80e-28 108
Msa1095620 MtrunA17_Chr4g0040931 50.847 118 57 1 1 117 492 609 1.43e-27 107
Msa1095620 MtrunA17_Chr7g0223761 44.000 125 69 1 1 124 213 337 2.14e-27 104
Msa1095620 MtrunA17_Chr7g0248131 46.154 130 68 1 1 128 494 623 2.61e-27 106
Msa1095620 MtrunA17_Chr7g0248151 43.846 130 71 1 1 128 492 621 3.19e-27 106
Msa1095620 MtrunA17_Chr7g0223751 45.312 128 70 0 1 128 491 618 4.11e-27 105
Msa1095620 MtrunA17_Chr1g0166551 45.238 126 67 1 1 124 376 501 4.16e-27 105
Msa1095620 MtrunA17_Chr7g0228281 46.400 125 66 1 1 124 508 632 5.63e-27 105
Msa1095620 MtrunA17_Chr7g0245531 47.200 125 66 0 1 125 189 313 8.10e-27 102
Msa1095620 MtrunA17_Chr7g0221661 45.902 122 66 0 1 122 492 613 1.99e-26 103
Msa1095620 MtrunA17_Chr7g0233311 46.400 125 66 1 1 124 493 617 2.17e-26 103
Msa1095620 MtrunA17_Chr1g0152001 45.763 118 63 1 1 117 176 293 2.93e-26 101
Msa1095620 MtrunA17_Chr2g0298851 46.400 125 65 2 1 124 493 616 1.31e-25 101
Msa1095620 MtrunA17_Chr7g0223621 45.299 117 64 0 1 117 491 607 5.31e-25 99.8
Msa1095620 MtrunA17_Chr7g0223781 45.902 122 66 0 1 122 490 611 5.34e-25 99.8
Msa1095620 MtrunA17_Chr7g0233301 43.307 127 71 1 1 126 462 588 8.72e-25 99.4
Msa1095620 MtrunA17_Chr2g0293581 46.400 125 66 1 1 124 494 618 9.90e-25 99.0
Msa1095620 MtrunA17_Chr7g0225671 44.000 125 69 1 1 124 498 622 1.14e-24 99.0
Msa1095620 MtrunA17_Chr7g0223871 40.800 125 73 1 1 124 8 132 1.62e-24 92.4
Msa1095620 MtrunA17_Chr7g0223741 48.077 104 54 0 1 104 492 595 1.66e-24 98.6
Msa1095620 MtrunA17_Chr7g0217971 45.872 109 59 0 1 109 467 575 8.84e-24 96.3
Msa1095620 MtrunA17_Chr7g0223721 42.400 125 71 1 1 124 492 616 1.12e-23 96.3
Msa1095620 MtrunA17_Chr2g0287631 44.800 125 68 1 1 124 493 617 1.37e-23 95.9
Msa1095620 MtrunA17_Chr4g0012541 40.800 125 73 1 1 124 483 607 1.64e-23 95.5
Msa1095620 MtrunA17_Chr7g0245541 40.800 125 74 0 1 125 76 200 3.10e-23 90.5
Msa1095620 MtrunA17_Chr5g0427901 41.129 124 73 0 1 124 503 626 9.72e-23 93.6
Msa1095620 MtrunA17_Chr7g0223801 38.710 124 76 0 1 124 490 613 5.41e-22 91.3
Msa1095620 MtrunA17_Chr7g0223851 40.800 125 73 1 1 124 501 625 5.84e-22 91.3
Msa1095620 MtrunA17_Chr1g0188611 43.200 125 63 2 1 124 171 288 8.79e-22 88.6
Msa1095620 MtrunA17_Chr7g0215261 39.200 125 74 1 1 123 458 582 6.88e-20 85.1
Msa1095620 MtrunA17_Chr7g0223831 37.903 124 76 1 1 123 464 587 2.55e-19 83.6
Msa1095620 MtrunA17_Chr5g0394221 44.792 96 48 1 1 96 261 351 1.80e-17 77.8
Msa1095620 MtrunA17_Chr7g0223821 41.600 125 70 1 1 122 493 617 1.80e-16 75.5
Msa1095620 MtrunA17_Chr6g0462861 37.037 135 74 3 1 124 471 605 8.31e-16 73.6
Msa1095620 MtrunA17_Chr2g0299821 72.727 55 8 1 1 48 430 484 8.69e-16 73.6
Msa1095620 MtrunA17_Chr6g0481381 39.394 99 59 1 24 121 75 173 3.00e-14 66.6
Msa1095620 MtrunA17_Chr7g0223791 41.463 82 48 0 1 82 100 181 4.01e-14 66.2
Msa1095620 MtrunA17_Chr2g0331381 55.357 56 25 0 1 56 427 482 6.72e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1095620 AT3G09440.1 82.911 158 26 1 1 157 492 649 4.08e-82 254
Msa1095620 AT3G09440.4 82.911 158 26 1 1 157 492 649 4.08e-82 254
Msa1095620 AT3G09440.2 82.911 158 26 1 1 157 492 649 4.08e-82 254
Msa1095620 AT3G09440.3 82.911 158 26 1 1 157 492 649 4.08e-82 254
Msa1095620 AT3G12580.1 86.875 160 17 3 1 157 492 650 6.09e-81 251
Msa1095620 AT5G02500.1 81.250 160 27 1 1 157 492 651 1.03e-80 251
Msa1095620 AT5G02490.1 79.630 162 28 2 1 157 492 653 1.36e-78 245
Msa1095620 AT1G16030.1 71.069 159 41 2 1 157 491 646 2.89e-69 221
Msa1095620 AT5G02500.2 76.471 136 29 1 25 157 386 521 3.03e-61 197
Msa1095620 AT1G56410.1 75.000 128 30 1 1 128 492 617 2.04e-57 189
Msa1095620 AT5G42020.1 54.348 138 61 2 1 136 517 654 4.77e-43 151
Msa1095620 AT5G42020.3 54.348 138 61 2 1 136 522 659 6.15e-43 150
Msa1095620 AT5G28540.1 53.906 128 58 1 1 127 517 644 4.98e-40 142
Msa1095620 AT1G09080.1 47.656 128 66 1 1 127 531 658 3.30e-27 106
Msa1095620 AT1G09080.2 47.656 128 66 1 1 127 521 648 3.68e-27 106
Msa1095620 AT5G42020.2 50.562 89 42 2 50 136 511 599 5.06e-23 94.7

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATCGACTTCTTCAATCTT+AGG 0.220026 7_3:-18627632 None:intergenic
AGGTGGAGGTGCTCCTGATA+TGG 0.335554 7_3:+18627562 Msa1095620:CDS
TGATACATCTTAGCAATGAT+TGG 0.339938 7_3:-18627539 None:intergenic
TCCATACCACCACCCATATC+AGG 0.393382 7_3:-18627575 None:intergenic
CCTCCAGCAGCAGGTGGTGC+TGG 0.395029 7_3:+18627608 Msa1095620:CDS
GGTTGATGCAAAGAATGCTT+TGG 0.410300 7_3:+18627331 Msa1095620:CDS
CGACAAAGGAAGGTTGTCTA+AGG 0.418046 7_3:+18627241 Msa1095620:CDS
ACAATTAAAGATGAGAAGAT+TGG 0.425467 7_3:+18627380 Msa1095620:CDS
TCTGCTGAAGACAAAACAAC+TGG 0.462288 7_3:+18627191 Msa1095620:CDS
GGTGGAGGTGCTCCTGATAT+GGG 0.464681 7_3:+18627563 Msa1095620:CDS
TAAGGAAGAAATTGAGAAGA+TGG 0.466344 7_3:+18627259 Msa1095620:CDS
ACTCATCAGCCTCTGCCAAC+TGG 0.471841 7_3:-18627478 None:intergenic
GGACCAGCACCACCTGCTGC+TGG 0.476038 7_3:-18627611 None:intergenic
TCCTGATATGGGTGGTGGTA+TGG 0.476764 7_3:+18627574 Msa1095620:CDS
CCTTCCTTTGTCGTTGGTGA+TGG 0.494022 7_3:-18627231 None:intergenic
AGAAATTGAGAAGATGGTTG+AGG 0.504892 7_3:+18627265 Msa1095620:CDS
TGACGCTGCAATTCAGTGGT+TGG 0.507402 7_3:+18627448 Msa1095620:CDS
ATCATTGCTAAGATGTATCA+AGG 0.508198 7_3:+18627542 Msa1095620:CDS
GATGAAGTTCCTCCAGCAGC+AGG 0.518012 7_3:+18627599 Msa1095620:CDS
GGTGCTCCTGATATGGGTGG+TGG 0.533527 7_3:+18627569 Msa1095620:CDS
TGAGTTTGAAGATAAGATGA+AGG 0.542099 7_3:+18627496 Msa1095620:CDS
AGACAACCTTCCTTTGTCGT+TGG 0.545078 7_3:-18627237 None:intergenic
GAAATACAAGGCAGAAGATG+AGG 0.548776 7_3:+18627295 Msa1095620:CDS
GGACAGTAACCAGTTGGCAG+AGG 0.580101 7_3:+18627469 Msa1095620:CDS
TAGCAATGATTGGATTGCAA+AGG 0.581264 7_3:-18627529 None:intergenic
CCAGCACCACCTGCTGCTGG+AGG 0.596593 7_3:-18627608 None:intergenic
CCATCACCAACGACAAAGGA+AGG 0.602128 7_3:+18627231 Msa1095620:CDS
GCTAAGATGTATCAAGGTGG+AGG 0.602520 7_3:+18627548 Msa1095620:CDS
AAAACTATGCTTACAACATG+AGG 0.603991 7_3:+18627354 Msa1095620:CDS
GTGGTTGGACAGTAACCAGT+TGG 0.608307 7_3:+18627463 Msa1095620:CDS
ATTACCATCACCAACGACAA+AGG 0.613058 7_3:+18627227 Msa1095620:CDS
TGGATCAAAGCTTTCACCAG+AGG 0.630537 7_3:+18627400 Msa1095620:CDS
GGAGGTGCTCCTGATATGGG+TGG 0.636751 7_3:+18627566 Msa1095620:CDS
TGAGGAAGCAGAGAAATACA+AGG 0.640542 7_3:+18627283 Msa1095620:CDS
GAAGTTCCTCCAGCAGCAGG+TGG 0.652201 7_3:+18627602 Msa1095620:CDS
CAATTGACGCTGCAATTCAG+TGG 0.657113 7_3:+18627444 Msa1095620:CDS
ATTGCTAAGATGTATCAAGG+TGG 0.733799 7_3:+18627545 Msa1095620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAATTAAAGATGAGAAGAT+TGG + chr7_3:18627380-18627399 Msa1095620:CDS 25.0%
TAAGGAAGAAATTGAGAAGA+TGG + chr7_3:18627259-18627278 Msa1095620:CDS 30.0%
AAAACTATGCTTACAACATG+AGG + chr7_3:18627354-18627373 Msa1095620:CDS 30.0%
TGAGTTTGAAGATAAGATGA+AGG + chr7_3:18627496-18627515 Msa1095620:CDS 30.0%
TGATACATCTTAGCAATGAT+TGG - chr7_3:18627542-18627561 None:intergenic 30.0%
ATCATTGCTAAGATGTATCA+AGG + chr7_3:18627542-18627561 Msa1095620:CDS 30.0%
! TAATCGACTTCTTCAATCTT+AGG - chr7_3:18627635-18627654 None:intergenic 30.0%
AGAAATTGAGAAGATGGTTG+AGG + chr7_3:18627265-18627284 Msa1095620:CDS 35.0%
AGATGAGGAACACAAAAAGA+AGG + chr7_3:18627310-18627329 Msa1095620:CDS 35.0%
! TCAATCTTCTTTTTGTCCTC+TGG - chr7_3:18627419-18627438 None:intergenic 35.0%
TAGCAATGATTGGATTGCAA+AGG - chr7_3:18627532-18627551 None:intergenic 35.0%
ATTGCTAAGATGTATCAAGG+TGG + chr7_3:18627545-18627564 Msa1095620:CDS 35.0%
TCTGCTGAAGACAAAACAAC+TGG + chr7_3:18627191-18627210 Msa1095620:CDS 40.0%
ATTACCATCACCAACGACAA+AGG + chr7_3:18627227-18627246 Msa1095620:CDS 40.0%
TGAGGAAGCAGAGAAATACA+AGG + chr7_3:18627283-18627302 Msa1095620:CDS 40.0%
GAAATACAAGGCAGAAGATG+AGG + chr7_3:18627295-18627314 Msa1095620:CDS 40.0%
! GGTTGATGCAAAGAATGCTT+TGG + chr7_3:18627331-18627350 Msa1095620:CDS 40.0%
AGACAACCTTCCTTTGTCGT+TGG - chr7_3:18627240-18627259 None:intergenic 45.0%
CGACAAAGGAAGGTTGTCTA+AGG + chr7_3:18627241-18627260 Msa1095620:CDS 45.0%
TGGATCAAAGCTTTCACCAG+AGG + chr7_3:18627400-18627419 Msa1095620:CDS 45.0%
CAATTGACGCTGCAATTCAG+TGG + chr7_3:18627444-18627463 Msa1095620:CDS 45.0%
GCTAAGATGTATCAAGGTGG+AGG + chr7_3:18627548-18627567 Msa1095620:CDS 45.0%
! CCTTCCTTTGTCGTTGGTGA+TGG - chr7_3:18627234-18627253 None:intergenic 50.0%
CCATCACCAACGACAAAGGA+AGG + chr7_3:18627231-18627250 Msa1095620:CDS 50.0%
TGACGCTGCAATTCAGTGGT+TGG + chr7_3:18627448-18627467 Msa1095620:CDS 50.0%
GTGGTTGGACAGTAACCAGT+TGG + chr7_3:18627463-18627482 Msa1095620:CDS 50.0%
! TCCTGATATGGGTGGTGGTA+TGG + chr7_3:18627574-18627593 Msa1095620:CDS 50.0%
TCCATACCACCACCCATATC+AGG - chr7_3:18627578-18627597 None:intergenic 50.0%
GGACAGTAACCAGTTGGCAG+AGG + chr7_3:18627469-18627488 Msa1095620:CDS 55.0%
ACTCATCAGCCTCTGCCAAC+TGG - chr7_3:18627481-18627500 None:intergenic 55.0%
!! AGGTGGAGGTGCTCCTGATA+TGG + chr7_3:18627562-18627581 Msa1095620:CDS 55.0%
!! GGTGGAGGTGCTCCTGATAT+GGG + chr7_3:18627563-18627582 Msa1095620:CDS 55.0%
GATGAAGTTCCTCCAGCAGC+AGG + chr7_3:18627599-18627618 Msa1095620:CDS 55.0%
!! GGAGGTGCTCCTGATATGGG+TGG + chr7_3:18627566-18627585 Msa1095620:CDS 60.0%
GGTGCTCCTGATATGGGTGG+TGG + chr7_3:18627569-18627588 Msa1095620:CDS 60.0%
GAAGTTCCTCCAGCAGCAGG+TGG + chr7_3:18627602-18627621 Msa1095620:CDS 60.0%
CCAGCACCACCTGCTGCTGG+AGG - chr7_3:18627611-18627630 None:intergenic 70.0%
CCTCCAGCAGCAGGTGGTGC+TGG + chr7_3:18627608-18627627 Msa1095620:CDS 70.0%
GGACCAGCACCACCTGCTGC+TGG - chr7_3:18627614-18627633 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 18627182 18627655 18627182 ID=Msa1095620;Name=Msa1095620
chr7_3 mRNA 18627182 18627655 18627182 ID=Msa1095620-mRNA-1;Parent=Msa1095620;Name=Msa1095620-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|157
chr7_3 exon 18627182 18627655 18627182 ID=Msa1095620-mRNA-1:exon:4024;Parent=Msa1095620-mRNA-1
chr7_3 CDS 18627182 18627655 18627182 ID=Msa1095620-mRNA-1:cds;Parent=Msa1095620-mRNA-1
Gene Sequence

>Msa1095620

TTGAATGTCTCTGCTGAAGACAAAACAACTGGACAGAAGAACAAGATTACCATCACCAACGACAAAGGAAGGTTGTCTAAGGAAGAAATTGAGAAGATGGTTGAGGAAGCAGAGAAATACAAGGCAGAAGATGAGGAACACAAAAAGAAGGTTGATGCAAAGAATGCTTTGGAAAACTATGCTTACAACATGAGGAACACAATTAAAGATGAGAAGATTGGATCAAAGCTTTCACCAGAGGACAAAAAGAAGATTGATGATGCAATTGACGCTGCAATTCAGTGGTTGGACAGTAACCAGTTGGCAGAGGCTGATGAGTTTGAAGATAAGATGAAGGAACTTGAGAGCCTTTGCAATCCAATCATTGCTAAGATGTATCAAGGTGGAGGTGCTCCTGATATGGGTGGTGGTATGGATGATGAAGTTCCTCCAGCAGCAGGTGGTGCTGGTCCTAAGATTGAAGAAGTCGATTAA

Protein sequence

>Msa1095620

LNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVEEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKIGSKLSPEDKKKIDDAIDAAIQWLDSNQLAEADEFEDKMKELESLCNPIIAKMYQGGGAPDMGGGMDDEVPPAAGGAGPKIEEVD*