AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0647380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647380 MtrunA17_Chr4g0038821 97.059 68 2 0 1 68 1 68 1.37e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647380 AT3G22210.1 62.500 64 23 1 5 68 6 68 1.55e-20 77.0

Find 20 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTCGCTCTTCCAGTTT+TGG 0.231911 4_3:+53473831 Msa0647380:CDS
CGAGGGAGAAAAGTCATTTA+AGG 0.295877 4_3:-53474428 None:intergenic
GTCTTCTAGCTCGCCTCTCT+CGG 0.346161 4_3:-53474061 None:intergenic
GACAGAAATCACTTCAAGAT+TGG 0.349085 4_3:+53473991 Msa0647380:intron
GCTGCAACAATGCCCAAAAC+TGG 0.365881 4_3:-53473844 None:intergenic
AGCTCGCCTCTCTCGGGAGC+TGG 0.377870 4_3:-53474054 None:intergenic
GAGAGATATATCATCAGAAA+TGG 0.429163 4_3:+53473801 Msa0647380:exon
AGTAGCAAATTGTTTGTACT+AGG 0.459932 4_3:+53474198 Msa0647380:three_prime_UTR
ACAGAAATCACTTCAAGATT+GGG 0.482353 4_3:+53473992 Msa0647380:intron
ATTGAAACTCACTGCATAGA+AGG 0.485260 4_3:-53473878 None:intergenic
TCTTCTAGCTCGCCTCTCTC+GGG 0.500761 4_3:-53474060 None:intergenic
ACCAACAAGAACTATTGAAA+AGG 0.500770 4_3:-53474229 None:intergenic
GGAGAAAAGTCATTTAAGGA+TGG 0.516791 4_3:-53474424 None:intergenic
CTCTCTCGGGAGCTGGACGT+TGG 0.528180 4_3:-53474047 None:intergenic
GATGATTCTGAATCAGAGAA+TGG 0.538128 4_3:+53474014 Msa0647380:CDS
GAGAAAAGTCATTTAAGGAT+GGG 0.540563 4_3:-53474423 None:intergenic
CAACGTCCAGCTCCCGAGAG+AGG 0.600475 4_3:+53474048 Msa0647380:CDS
GAGTTTCAATGAGATTAGAG+AGG 0.641117 4_3:+53473891 Msa0647380:CDS
GATTCTGAATCAGAGAATGG+AGG 0.645594 4_3:+53474017 Msa0647380:CDS
GTGAGACTAGTCACTGACCC+AGG 0.670217 4_3:-53474350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACATTTTTCTAATAGAAAA+TGG - chr4_3:53474278-53474297 None:intergenic 15.0%
!!! CTGTCATAATTCAATTTTAT+AGG - chr4_3:53473976-53473995 None:intergenic 20.0%
!!! ATTTCTTTATTTTTGATGCT+AGG + chr4_3:53474158-53474177 Msa0647380:three_prime_UTR 20.0%
!!! TTGCTGTTGAAGTTATTTTT+TGG - chr4_3:53474102-53474121 None:intergenic 25.0%
GAGAGATATATCATCAGAAA+TGG + chr4_3:53473801-53473820 Msa0647380:exon 30.0%
ACAGAAATCACTTCAAGATT+GGG + chr4_3:53473992-53474011 Msa0647380:intron 30.0%
AGTAGCAAATTGTTTGTACT+AGG + chr4_3:53474198-53474217 Msa0647380:three_prime_UTR 30.0%
! ACCTTTTCAATAGTTCTTGT+TGG + chr4_3:53474228-53474247 Msa0647380:three_prime_UTR 30.0%
ACCAACAAGAACTATTGAAA+AGG - chr4_3:53474232-53474251 None:intergenic 30.0%
!!! ACATTTTTTGTGTTTTCTCC+TGG + chr4_3:53474332-53474351 Msa0647380:three_prime_UTR 30.0%
!!! CATTTTTTGTGTTTTCTCCT+GGG + chr4_3:53474333-53474352 Msa0647380:three_prime_UTR 30.0%
!!! CATGCAACTATTTTAATCTC+TGG - chr4_3:53474390-53474409 None:intergenic 30.0%
ATTGAAACTCACTGCATAGA+AGG - chr4_3:53473881-53473900 None:intergenic 35.0%
GAGTTTCAATGAGATTAGAG+AGG + chr4_3:53473891-53473910 Msa0647380:CDS 35.0%
GACAGAAATCACTTCAAGAT+TGG + chr4_3:53473991-53474010 Msa0647380:intron 35.0%
GATGATTCTGAATCAGAGAA+TGG + chr4_3:53474014-53474033 Msa0647380:CDS 35.0%
! CAATTCGCTCTTCCAGTTTT+GGG + chr4_3:53473832-53473851 Msa0647380:CDS 40.0%
GATTCTGAATCAGAGAATGG+AGG + chr4_3:53474017-53474036 Msa0647380:CDS 40.0%
! GCAATTCGCTCTTCCAGTTT+TGG + chr4_3:53473831-53473850 Msa0647380:CDS 45.0%
GCTGCAACAATGCCCAAAAC+TGG - chr4_3:53473847-53473866 None:intergenic 50.0%
!! TCTTCTAGCTCGCCTCTCTC+GGG - chr4_3:53474063-53474082 None:intergenic 55.0%
!! GTCTTCTAGCTCGCCTCTCT+CGG - chr4_3:53474064-53474083 None:intergenic 55.0%
! GTGAGACTAGTCACTGACCC+AGG - chr4_3:53474353-53474372 None:intergenic 55.0%
CTCTCTCGGGAGCTGGACGT+TGG - chr4_3:53474050-53474069 None:intergenic 65.0%
CAACGTCCAGCTCCCGAGAG+AGG + chr4_3:53474048-53474067 Msa0647380:CDS 65.0%
AGCTCGCCTCTCTCGGGAGC+TGG - chr4_3:53474057-53474076 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 53473770 53474444 53473770 ID=Msa0647380;Name=Msa0647380
chr4_3 mRNA 53473770 53474444 53473770 ID=Msa0647380-mRNA-1;Parent=Msa0647380;Name=Msa0647380-mRNA-1;_AED=0.05;_eAED=0.05;_QI=50|1|1|1|1|1|2|335|68
chr4_3 exon 53473770 53473912 53473770 ID=Msa0647380-mRNA-1:exon:13330;Parent=Msa0647380-mRNA-1
chr4_3 exon 53473996 53474444 53473996 ID=Msa0647380-mRNA-1:exon:13331;Parent=Msa0647380-mRNA-1
chr4_3 five_prime_UTR 53473770 53473819 53473770 ID=Msa0647380-mRNA-1:five_prime_utr;Parent=Msa0647380-mRNA-1
chr4_3 CDS 53473820 53473912 53473820 ID=Msa0647380-mRNA-1:cds;Parent=Msa0647380-mRNA-1
chr4_3 CDS 53473996 53474109 53473996 ID=Msa0647380-mRNA-1:cds;Parent=Msa0647380-mRNA-1
chr4_3 three_prime_UTR 53474110 53474444 53474110 ID=Msa0647380-mRNA-1:three_prime_utr;Parent=Msa0647380-mRNA-1
Gene Sequence

>Msa0647380

ATGGATGCAATGCAATTCGCTCTTCCAGTTTTGGGCATTGTTGCAGCTGCTGCTGTGACCTTCTATGCAGTGAGTTTCAATGAGATTAGAGAGAAATCACTTCAAGATTGGGATGATTCTGAATCAGAGAATGGAGGTTTTAGACCAACGTCCAGCTCCCGAGAGAGGCGAGCTAGAAGACAAGCTAGTAAAAATACCAAAAAATAA

Protein sequence

>Msa0647380

MDAMQFALPVLGIVAAAAVTFYAVSFNEIREKSLQDWDDSESENGGFRPTSSSRERRARRQASKNTKK*