AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1341070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1341070 MtrunA17_Chr8g0372561 90.306 196 18 1 1 196 1 195 5.88e-107 305
Msa1341070 MtrunA17_Chr7g0234831 50.000 148 64 3 50 196 79 217 5.19e-33 117
Msa1341070 MtrunA17_Chr2g0293431 46.358 151 66 2 43 191 13 150 3.94e-32 114
Msa1341070 MtrunA17_Chr2g0310131 48.299 147 68 2 50 196 78 216 1.38e-31 114
Msa1341070 MtrunA17_Chr2g0293341 46.250 160 69 4 41 196 44 190 1.03e-30 110
Msa1341070 MtrunA17_Chr6g0450121 33.824 136 72 1 60 195 63 180 8.18e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1341070 AT4G36420.1 51.613 186 77 4 17 196 1 179 7.77e-50 160
Msa1341070 AT1G70190.2 48.649 148 72 1 49 196 65 208 2.98e-33 118
Msa1341070 AT1G70190.1 48.649 148 72 1 49 196 65 208 2.98e-33 118
Msa1341070 AT3G06040.3 63.441 93 30 1 104 196 98 186 2.85e-29 107
Msa1341070 AT3G06040.2 63.441 93 30 1 104 196 98 186 2.85e-29 107
Msa1341070 AT3G06040.1 63.441 93 30 1 104 196 98 186 2.85e-29 107
Msa1341070 AT4G37660.1 42.857 161 74 3 38 196 23 167 4.58e-25 96.3
Msa1341070 AT3G27850.1 38.194 144 73 1 52 195 59 186 3.33e-18 79.0
Msa1341070 AT3G27830.2 38.194 144 73 1 52 195 63 190 3.36e-18 79.0
Msa1341070 AT3G27830.1 38.194 144 73 1 52 195 63 190 3.36e-18 79.0
Msa1341070 AT2G03130.1 49.438 89 41 1 108 196 7 91 5.97e-16 70.9
Msa1341070 AT3G27840.1 30.682 176 104 2 23 195 32 192 2.01e-15 71.6

Find 52 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGTGAGAAAGGAAATT+AGG 0.194585 tig0004716:+5273 None:intergenic
TGAAGTGATGCGTGAGAAAA+AGG 0.246427 tig0004716:-5109 Msa1341070:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.262636 tig0004716:-5056 Msa1341070:CDS
TGATGCGGCGTCAAAGATTA+AGG 0.294083 tig0004716:-4923 Msa1341070:CDS
AGTTGGTGCTAAAGCTTCTA+TGG 0.325399 tig0004716:-4767 Msa1341070:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 tig0004716:-5022 Msa1341070:CDS
TTTGGTGAGAAAGGAAATTA+GGG 0.344047 tig0004716:+5274 None:intergenic
TGCGGAGATGTGAGAATTGT+AGG 0.346963 tig0004716:+5313 None:intergenic
TTAAGGTTATTAAAGAAGTT+AGG 0.362138 tig0004716:-4906 Msa1341070:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 tig0004716:-5055 Msa1341070:CDS
GCGTTTGATGTGAAGCTTGA+TGG 0.371046 tig0004716:-4949 Msa1341070:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 tig0004716:+5051 None:intergenic
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 tig0004716:-5021 Msa1341070:CDS
ATTATGATGTTGATGGTTCC+TGG 0.398854 tig0004716:-5069 Msa1341070:CDS
GCTTCCGATTATGATGTTGA+TGG 0.408175 tig0004716:-5076 Msa1341070:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 tig0004716:-5024 Msa1341070:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 tig0004716:-4866 Msa1341070:CDS
GGAACCATCAACATCATAAT+CGG 0.459221 tig0004716:+5072 None:intergenic
ATTGCTAAGTTGAAGGAAGT+TGG 0.461667 tig0004716:-4784 Msa1341070:CDS
GGGGATTATTGCTAAGTTGA+AGG 0.470094 tig0004716:-4791 Msa1341070:CDS
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 tig0004716:-5023 Msa1341070:CDS
TATGGAGGTGGATTTGCATT+GGG 0.481396 tig0004716:+5230 None:intergenic
GTATGGAGGTGGATTTGCAT+TGG 0.489491 tig0004716:+5229 None:intergenic
TTTGCATTGGGAGAGGATGC+AGG 0.489536 tig0004716:+5242 None:intergenic
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 tig0004716:-5030 Msa1341070:CDS
TGTGACGAAGGAAGAGGCCG+AGG 0.503668 tig0004716:-4812 Msa1341070:CDS
GATGTTGATGGTTCCTGGAA+TGG 0.504794 tig0004716:-5064 Msa1341070:CDS
TTCGGGATTCGGTGAATCGG+TGG 0.512716 tig0004716:+5194 None:intergenic
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 tig0004716:-4872 Msa1341070:CDS
GGTGGTGTAATTGCGGCGTA+TGG 0.529938 tig0004716:+5212 None:intergenic
GTAATTGCGGCGTATGGAGG+TGG 0.535225 tig0004716:+5218 None:intergenic
ATGTTGATGGTTCCTGGAAT+GGG 0.541612 tig0004716:-5063 Msa1341070:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 tig0004716:-5036 Msa1341070:CDS
AGGTGGATTTGCATTGGGAG+AGG 0.544913 tig0004716:+5235 None:intergenic
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 tig0004716:-4863 Msa1341070:CDS
CAACTTAGCAATAATCCCCT+CGG 0.570601 tig0004716:+4795 None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG 0.571665 tig0004716:-4980 Msa1341070:CDS
GAAAGGTGTGACGAAGGAAG+AGG 0.571984 tig0004716:-4818 Msa1341070:CDS
GGTGTAATTGCGGCGTATGG+AGG 0.572681 tig0004716:+5215 None:intergenic
TTCCCTCCACCTACACCTCT+CGG 0.576289 tig0004716:+5027 None:intergenic
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 tig0004716:-5029 Msa1341070:CDS
ATTAACGCTTCTCGAAGCAA+TGG 0.603358 tig0004716:-5139 Msa1341070:CDS
GTGAATCGGTGGTGTAATTG+CGG 0.609756 tig0004716:+5205 None:intergenic
GTGACGAAGGAAGAGGCCGA+GGG 0.612413 tig0004716:-4811 Msa1341070:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 tig0004716:-5033 Msa1341070:CDS
GCAATCGTCGACGAACTCGC+GGG 0.626410 tig0004716:-5162 Msa1341070:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 tig0004716:+4832 None:intergenic
AGCAATCGTCGACGAACTCG+CGG 0.632941 tig0004716:-5163 Msa1341070:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 tig0004716:-5054 Msa1341070:CDS
TTTGAAGAAAGGTGTGACGA+AGG 0.649602 tig0004716:-4824 Msa1341070:CDS
TGACGAAGGAAGAGGCCGAG+GGG 0.703464 tig0004716:-4810 Msa1341070:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 tig0004716:-5042 Msa1341070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGGTTATTAAAGAAGTT+AGG - tig0004716:5186-5205 Msa1341070:CDS 20.0%
!!! GAAATTAGGGTTTTGAATTA+GGG + tig0004716:4808-4827 None:intergenic 25.0%
!!! GGAAATTAGGGTTTTGAATT+AGG + tig0004716:4809-4828 None:intergenic 30.0%
TTTGGTGAGAAAGGAAATTA+GGG + tig0004716:4821-4840 None:intergenic 30.0%
! ATTTGGTGAGAAAGGAAATT+AGG + tig0004716:4822-4841 None:intergenic 30.0%
!!! TTTTTGGAATTTGGTGAGAA+AGG + tig0004716:4830-4849 None:intergenic 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG - tig0004716:5202-5221 Msa1341070:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - tig0004716:5203-5222 Msa1341070:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - tig0004716:5204-5223 Msa1341070:CDS 30.0%
!!! GGTTTTGAATTAGGGTTTTG+CGG + tig0004716:4800-4819 None:intergenic 35.0%
!!! ACGATTGCTGATACTTTTTC+GGG + tig0004716:4918-4937 None:intergenic 35.0%
!! GACGATTGCTGATACTTTTT+CGG + tig0004716:4919-4938 None:intergenic 35.0%
GGAACCATCAACATCATAAT+CGG + tig0004716:5023-5042 None:intergenic 35.0%
!! ATTATGATGTTGATGGTTCC+TGG - tig0004716:5023-5042 Msa1341070:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - tig0004716:5211-5230 Msa1341070:CDS 35.0%
! ATTGCTAAGTTGAAGGAAGT+TGG - tig0004716:5308-5327 Msa1341070:CDS 35.0%
!!! GGATGCAGGTTTTTGGAATT+TGG + tig0004716:4839-4858 None:intergenic 40.0%
! TATGGAGGTGGATTTGCATT+GGG + tig0004716:4865-4884 None:intergenic 40.0%
!!! TTTTTCGGGATTCGGTGAAT+CGG + tig0004716:4904-4923 None:intergenic 40.0%
!!! GCTGATACTTTTTCGGGATT+CGG + tig0004716:4912-4931 None:intergenic 40.0%
ATTAACGCTTCTCGAAGCAA+TGG - tig0004716:4953-4972 Msa1341070:CDS 40.0%
TGAAGTGATGCGTGAGAAAA+AGG - tig0004716:4983-5002 Msa1341070:CDS 40.0%
GAAGTGATGCGTGAGAAAAA+GGG - tig0004716:4984-5003 Msa1341070:CDS 40.0%
GCTTCCGATTATGATGTTGA+TGG - tig0004716:5016-5035 Msa1341070:CDS 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG - tig0004716:5029-5048 Msa1341070:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - tig0004716:5154-5173 Msa1341070:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - tig0004716:5226-5245 Msa1341070:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + tig0004716:5263-5282 None:intergenic 40.0%
! TTTGAAGAAAGGTGTGACGA+AGG - tig0004716:5268-5287 Msa1341070:CDS 40.0%
CAACTTAGCAATAATCCCCT+CGG + tig0004716:5300-5319 None:intergenic 40.0%
! GGGGATTATTGCTAAGTTGA+AGG - tig0004716:5301-5320 Msa1341070:CDS 40.0%
!! AGTTGGTGCTAAAGCTTCTA+TGG - tig0004716:5325-5344 Msa1341070:CDS 40.0%
TGCGGAGATGTGAGAATTGT+AGG + tig0004716:4782-4801 None:intergenic 45.0%
! GTATGGAGGTGGATTTGCAT+TGG + tig0004716:4866-4885 None:intergenic 45.0%
GTGAATCGGTGGTGTAATTG+CGG + tig0004716:4890-4909 None:intergenic 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG - tig0004716:5028-5047 Msa1341070:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG - tig0004716:5036-5055 Msa1341070:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - tig0004716:5037-5056 Msa1341070:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - tig0004716:5038-5057 Msa1341070:CDS 45.0%
ATTCCCCTTATTCCCATTCC+AGG + tig0004716:5044-5063 None:intergenic 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - tig0004716:5143-5162 Msa1341070:CDS 45.0%
TGATGCGGCGTCAAAGATTA+AGG - tig0004716:5169-5188 Msa1341070:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - tig0004716:5220-5239 Msa1341070:CDS 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - tig0004716:5229-5248 Msa1341070:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - tig0004716:5257-5276 Msa1341070:CDS 45.0%
! TGGGAGAGGATGCAGGTTTT+TGG + tig0004716:4846-4865 None:intergenic 50.0%
TTTGCATTGGGAGAGGATGC+AGG + tig0004716:4853-4872 None:intergenic 50.0%
! AGGTGGATTTGCATTGGGAG+AGG + tig0004716:4860-4879 None:intergenic 50.0%
! GGGGTGGTGGTAGTGAAAAA+GGG - tig0004716:5090-5109 Msa1341070:CDS 50.0%
! GGGTGGTGGTAGTGAAAAAG+GGG - tig0004716:5091-5110 Msa1341070:CDS 50.0%
! GGTGGTGGTAGTGAAAAAGG+GGG - tig0004716:5092-5111 Msa1341070:CDS 50.0%
! TGGTAGTGAAAAAGGGGGTG+AGG - tig0004716:5097-5116 Msa1341070:CDS 50.0%
GGGTGAGGTGAAGAAAGTTG+AGG - tig0004716:5112-5131 Msa1341070:CDS 50.0%
! GAAAGGTGTGACGAAGGAAG+AGG - tig0004716:5274-5293 Msa1341070:CDS 50.0%
GTAATTGCGGCGTATGGAGG+TGG + tig0004716:4877-4896 None:intergenic 55.0%
GGTGTAATTGCGGCGTATGG+AGG + tig0004716:4880-4899 None:intergenic 55.0%
GGTGGTGTAATTGCGGCGTA+TGG + tig0004716:4883-4902 None:intergenic 55.0%
!! TTCGGGATTCGGTGAATCGG+TGG + tig0004716:4901-4920 None:intergenic 55.0%
AGCAATCGTCGACGAACTCG+CGG - tig0004716:4929-4948 Msa1341070:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - tig0004716:5050-5069 Msa1341070:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + tig0004716:5068-5087 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - tig0004716:5068-5087 Msa1341070:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - tig0004716:5069-5088 Msa1341070:CDS 55.0%
AGGTGTAGGTGGAGGGAAAG+GGG - tig0004716:5070-5089 Msa1341070:CDS 55.0%
! GGGGGTGGTGGTAGTGAAAA+AGG - tig0004716:5089-5108 Msa1341070:CDS 55.0%
GCAATCGTCGACGAACTCGC+GGG - tig0004716:4930-4949 Msa1341070:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - tig0004716:5056-5075 Msa1341070:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - tig0004716:5059-5078 Msa1341070:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - tig0004716:5062-5081 Msa1341070:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - tig0004716:5063-5082 Msa1341070:CDS 60.0%
GGTGTAGGTGGAGGGAAAGG+GGG - tig0004716:5071-5090 Msa1341070:CDS 60.0%
TGTGACGAAGGAAGAGGCCG+AGG - tig0004716:5280-5299 Msa1341070:CDS 60.0%
GTGACGAAGGAAGAGGCCGA+GGG - tig0004716:5281-5300 Msa1341070:CDS 60.0%
TGACGAAGGAAGAGGCCGAG+GGG - tig0004716:5282-5301 Msa1341070:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - tig0004716:5074-5093 Msa1341070:CDS 65.0%
GGTGGAGGGAAAGGGGGTGG+TGG - tig0004716:5077-5096 Msa1341070:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0004716 gene 4762 5352 4762 ID=Msa1341070;Name=Msa1341070
tig0004716 mRNA 4762 5352 4762 ID=Msa1341070-mRNA-1;Parent=Msa1341070;Name=Msa1341070-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|196
tig0004716 exon 4762 5352 4762 ID=Msa1341070-mRNA-1:exon:2972;Parent=Msa1341070-mRNA-1
tig0004716 CDS 4762 5352 4762 ID=Msa1341070-mRNA-1:cds;Parent=Msa1341070-mRNA-1
Gene Sequence

>Msa1341070

ATGAAGTTTTCACATTTCCTACAATTCTCACATCTCCGCAAAACCCTAATTCAAAACCCTAATTTCCTTTCTCACCAAATTCCAAAAACCTGCATCCTCTCCCAATGCAAATCCACCTCCATACGCCGCAATTACACCACCGATTCACCGAATCCCGAAAAAGTATCAGCAATCGTCGACGAACTCGCGGGATTAACGCTTCTCGAAGCAATGGATCTCGTTGAAGTGATGCGTGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGGTAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAGGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAGGAAGAGGCCGAGGGGATTATTGCTAAGTTGAAGGAAGTTGGTGCTAAAGCTTCTATGGAATAA

Protein sequence

>Msa1341070

MKFSHFLQFSHLRKTLIQNPNFLSHQIPKTCILSQCKSTSIRRNYTTDSPNPEKVSAIVDELAGLTLLEAMDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGGSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME*