AlfalfaGEDB | Msa1214020

M. sativa cultivar zhongmu-4 / Msa1214020

KEGG: K19589
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363
PFAM: PF13041.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1214020	sp\|Q9SJZ3\|PP169_ARATH	56.995	579	230	2	1	560	76	654	0.0	678

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1214020	Q2HS71	80.157	892	89	6	1	804	80	971	0.0	1405

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa1214020	0.812506	4.095974e-51	-8.615850e-47
Msa0064350	Msa1214020	0.800413	1.458480e-48	-8.615850e-47
Msa0120040	Msa1214020	-0.807967	3.902505e-50	-8.615850e-47
Msa0157290	Msa1214020	0.812775	3.577957e-51	-8.615850e-47
Msa0191050	Msa1214020	0.800486	1.409350e-48	-8.615850e-47
Msa0220580	Msa1214020	0.803289	3.742394e-49	-8.615850e-47
Msa0233350	Msa1214020	0.805138	1.542644e-49	-8.615850e-47
Msa0243100	Msa1214020	0.805169	1.519715e-49	-8.615850e-47
Msa0287650	Msa1214020	0.828476	8.802751e-55	-8.615850e-47
Msa0298700	Msa1214020	0.809909	1.498779e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1214020	MtrunA17_Chr4g0011431	95.720	257	11	0	548	804	1	257	6.61e-176	505
Msa1214020	MtrunA17_Chr7g0259141	40.171	585	317	6	1	560	67	643	2.86e-143	437
Msa1214020	MtrunA17_Chr4g0003141	38.448	580	335	5	2	560	59	637	4.12e-143	439
Msa1214020	MtrunA17_Chr5g0403151	36.907	569	339	3	1	552	59	624	1.06e-124	389
Msa1214020	MtrunA17_Chr3g0099291	35.368	639	334	6	1	560	40	678	1.61e-123	389
Msa1214020	MtrunA17_Chr6g0488851	36.542	561	333	5	4	542	28	587	1.13e-122	382
Msa1214020	MtrunA17_Chr4g0063341	35.809	606	302	8	1	552	51	623	1.82e-115	367
Msa1214020	MtrunA17_Chr7g0247091	35.609	542	321	5	21	541	21	555	3.53e-113	355
Msa1214020	MtrunA17_Chr5g0394691	32.630	616	390	4	1	594	158	770	2.38e-110	356
Msa1214020	MtrunA17_Chr5g0394691	29.289	478	286	8	11	481	61	493	1.43e-55	205

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1214020	AT2G22410.1	56.995	579	230	2	1	560	76	654	0.0	678
Msa1214020	AT2G29760.1	35.802	567	346	2	1	549	53	619	4.59e-139	429
Msa1214020	AT3G15930.1	35.445	584	344	4	2	560	58	633	1.42e-124	389
Msa1214020	AT1G08070.1	35.843	611	309	7	1	560	56	634	2.62e-119	377
Msa1214020	AT1G31430.2	37.406	532	310	5	38	548	6	535	6.30e-118	368
Msa1214020	AT1G31430.2	25.773	388	224	5	33	388	102	457	4.97e-31	129
Msa1214020	AT1G31430.1	37.406	532	310	5	38	548	6	535	6.30e-118	368
Msa1214020	AT1G31430.1	25.773	388	224	5	33	388	102	457	4.97e-31	129
Msa1214020	AT3G22690.3	35.818	550	336	3	1	549	160	693	1.95e-109	353
Msa1214020	AT3G22690.3	28.008	507	311	10	5	498	59	524	2.70e-55	204

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Find 201 sgRNAs with CRISPR-Local

Find 315 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGACCAATCTACTTCT+GGG	0.469402	8_1:-74490881	Msa1214020:CDS
AAAGGAGTAGAGAAGACTCC+TGG	0.576572	8_1:-74493129	Msa1214020:CDS
AAATTCCTCTACTCTCATAC+TGG	0.446075	8_1:-74490836	Msa1214020:CDS
AACAGCAGCATCTGCTGCCA+TGG	0.433130	8_1:+74493332	None:intergenic
AACATCCAGAAAACCAAATC+TGG	0.417771	8_1:+74493984	None:intergenic
AAGCCGAAGTCGGGTCTGAA+GGG	0.566767	8_1:-74491154	Msa1214020:CDS
AAGGGGTAAGTATGATTATC+CGG	0.457475	8_1:+74494525	None:intergenic
AAGTGCTAAACCACAAATCA+CGG	0.604520	8_1:+74493606	None:intergenic
AATAACCTTTAAGCAACAAG+GGG	0.663744	8_1:+74494508	None:intergenic
AATCCCTTCAGACCCGACTT+CGG	0.573665	8_1:+74491151	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGAGTGGAAATATAGAA+GGG	-	chr8_1:74490681-74490700	Msa1214020:three_prime_UTR	30.0%
TTGTATAAGAGAATGTTGCT+AGG	-	chr8_1:74490713-74490732	Msa1214020:three_prime_UTR	30.0%
AATAACCTTTAAGCAACAAG+GGG	+	chr8_1:74490769-74490788	None:intergenic	30.0%
CAATAACCTTTAAGCAACAA+GGG	+	chr8_1:74490770-74490789	None:intergenic	30.0%
ACAATAACCTTTAAGCAACA+AGG	+	chr8_1:74490771-74490790	None:intergenic	30.0%
CAAAATCTATAGGGAAATGA+AGG	-	chr8_1:74491015-74491034	Msa1214020:CDS	30.0%
CAAATGAGATTACGATGATT+GGG	-	chr8_1:74491053-74491072	Msa1214020:CDS	30.0%
GATTGTGCCTTTCAATATAA+CGG	+	chr8_1:74491528-74491547	None:intergenic	30.0%
TGAGTAGTATTTAAGCTTAG+GGG	+	chr8_1:74491860-74491879	None:intergenic	30.0%
CATGAGTAGTATTTAAGCTT+AGG	+	chr8_1:74491862-74491881	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	74490521	74494775	74490521	ID=Msa1214020;Name=Msa1214020
chr8_1	mRNA	74490521	74494775	74490521	ID=Msa1214020-mRNA-1;Parent=Msa1214020;Name=Msa1214020-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0\|0.33\|1\|1\|1\|3\|285\|804
chr8_1	exon	74490521	74491576	74490521	ID=Msa1214020-mRNA-1:exon:25547;Parent=Msa1214020-mRNA-1
chr8_1	exon	74493086	74493397	74493086	ID=Msa1214020-mRNA-1:exon:25546;Parent=Msa1214020-mRNA-1
chr8_1	exon	74493444	74494775	74493444	ID=Msa1214020-mRNA-1:exon:25545;Parent=Msa1214020-mRNA-1
chr8_1	CDS	74493444	74494775	74493444	ID=Msa1214020-mRNA-1:cds;Parent=Msa1214020-mRNA-1
chr8_1	CDS	74493086	74493397	74493086	ID=Msa1214020-mRNA-1:cds;Parent=Msa1214020-mRNA-1
chr8_1	CDS	74490806	74491576	74490806	ID=Msa1214020-mRNA-1:cds;Parent=Msa1214020-mRNA-1
chr8_1	three_prime_UTR	74490521	74490805	74490521	ID=Msa1214020-mRNA-1:three_prime_utr;Parent=Msa1214020-mRNA-1

Gene Sequence

>Msa1214020

ATGGTCTTGACGGGATTGATTGAAAATAGGTTTGCTGCGAGTCGCCTTGTTGCATTCTGTGCTTTATCTGAGTCGAAAGAACTTGATTATTGTACTAGAATATTGTATCGAATTAAAGAACCGAATGTCTTTTCTTGGAATGCGGCAATTAGAGGGTATGTTGAGAGTGGAAATATAGAAGGGGGTTTTATGTTGTATAAGAGAATGTTGCTAGGTGGTACGTTGAAACCGGATAATCATACTTACCCCTTGTTGCTTAAAGGTTATTGTGGTCAATCTTTAAGTTGTGTCGGTCTTGGTATACTTGGGCATGTGTTGAAGTTTGGGTTTGAATGTGATATATTTGTGCACAATGCATCGATTACTATGCTACTCTGGTGTGGGGAGTTGAGTGTGGCTTATGACGTGTTTAATAAAAGTCGTGTGAGAGACCTCGTAACCTGGAATTCAATGATTACTGGGTGTGTTAAAAGGGGACTGGCAAACGAGGCTATCAAAATCTATAGGGAAATGAAGGCTGAGAATGTGAGACCAAATGAGATTACGATGATTGGGATGATTTCTTCTTGTTCTCAGTTGCAAGATTTGACTCTTGGCAAAGAATTTCATTGTTACATCGAAGAACACGGTCTTGAGTTTACAATTCCACTCACTAATGCACTTATGGACATGTATGTGAAGTGTGGGGAATTGTTGGCTGCACGAGTTCTATTTGATAACATGGCACAGAAGACTCTTGTTTCATGGACTACAATGGTTTTGGGATATGCCAGATTTGGTTTTCTGGATGTTGCTCGAGAGATTTTATATAAAGTTCCAGAAAAGAGTGTTGTGCCGTGGAATGCCATCATCAGTGGCAGTGTCCAGGCGAAGCATGGTAAAGAGGCATTGGCTCTATTCCATGAAATGCAAACTAGTAAAATAGAACTTGATAAAGTAACTATGGTTAACTGCCTGTCAGCATGTTCACAGCTAGGAGCACTTGATGTTGGAATATGGATTCACCGTTATATTGAAAGGCACAATCTTTCTATTGATGTCGCCTTAGGAACTGCACTAGTCGACATGTATGCCAAGTGTGGTAATATTGCAAGGGCGCTCCAGGTTTTCGAAGAGATTCCTCAAAGAAATTGTTTGACCTGGACAGCCGTGATTTGTGGTTTAGCACTTCATGGTAATGCTCAAGATGCTATATCCTATTTCTCAAAAATAATTCATATTGGAATAGTGCCTGATGAGATCACCTTTCTTGGTGTGTTATCAGCTTGTTGTCATGGAGGTTTAGTTGAAGAAGGCCACAAATATTTTTCCGAAATGAGCTCTAAATTCAATGAGGGTCATTTGGAGGAAGCAGAAGAACTTGTTAAAAATATGCCCATGGCAGCAGATGCTGCTGTTTTGGGTGCTTTATTCTTTGCTTGCCGTGTTTATGGAAATGTTCAAATTGGTGAGAGGACAGCTTTTAAGCTTCTTGAGATTGATCCTCAAGATAGTGGGAACTATGTTCTGCTTGCTAGCATGTACAGTGAGGCAAAAATGTGGAAGGAGGCAAGGAGTGCAAGGAAACTAATGAATGATAAAGGAGTAGAGAAGACTCCTGGTTGCAGCTTGGTTGAGATCAATGGCATTGTGCACGAGTTCGTGGCTTCATTCAAGGATTTATCTAAAACTGCCCAAATTTGCCTTGTGAGTTCCCACAACGAGGTTTATGAACCATGTGATGATTCTTTTGCACTTGTAGACGCTCTTCTAGCCGACCGCACTAACCTATTGGAACATCATCTAGCACTGTGCATGGAAATAGGCTGTGGAAGTGGATATGTTATTACTTCCCTGGCTCTTATTCTTGGGCAGGAAGGTAGTGGCGTAAATTACATTGCAACTGATATAAACCCACATGCAGTGAAAGTGACACGCGAGACAATGGAAGCACATGGAGTTGATGCAGAGTTGATAATAACCGATATTGCATCGGGACTTGAGAACCGACTAGCAGGGATGATTGATGTAATGGTTGTGAACCCTCCTTATGTTCCTACACCTGAAGCCGAAGTCGGGTCTGAAGGGATTACTGCTTCTTGGGCTGGTGGTGAGAATGGAAGGAGTGTAATTGATAGAATTTTGCCTGTTGCAGACGCTCTATTGTCAGAAAAAGGATGGTTGTATATGGTCACTCTCACAGCAAATAATCCTTCTGAAATTTGCCTTGAAATGAGAAAGAAAGGATATGCTTCTAAGATTGTTGTTCAAAGATCGACAGAGGAAGAAAGTCTACATATTATCAAATTCTGGAGGGATTTAGATAATAAATTAGATGAAAATGACCAATCTACTTCTGGGTTCATGGGATCTTTGCTTTCACAAATTCCTCTACTCTCATACTGGAGAGGCAACAATAGTGACGGCAAGTGTTGA

Protein sequence

>Msa1214020

MVLTGLIENRFAASRLVAFCALSESKELDYCTRILYRIKEPNVFSWNAAIRGYVESGNIEGGFMLYKRMLLGGTLKPDNHTYPLLLKGYCGQSLSCVGLGILGHVLKFGFECDIFVHNASITMLLWCGELSVAYDVFNKSRVRDLVTWNSMITGCVKRGLANEAIKIYREMKAENVRPNEITMIGMISSCSQLQDLTLGKEFHCYIEEHGLEFTIPLTNALMDMYVKCGELLAARVLFDNMAQKTLVSWTTMVLGYARFGFLDVAREILYKVPEKSVVPWNAIISGSVQAKHGKEALALFHEMQTSKIELDKVTMVNCLSACSQLGALDVGIWIHRYIERHNLSIDVALGTALVDMYAKCGNIARALQVFEEIPQRNCLTWTAVICGLALHGNAQDAISYFSKIIHIGIVPDEITFLGVLSACCHGGLVEEGHKYFSEMSSKFNEGHLEEAEELVKNMPMAADAAVLGALFFACRVYGNVQIGERTAFKLLEIDPQDSGNYVLLASMYSEAKMWKEARSARKLMNDKGVEKTPGCSLVEINGIVHEFVASFKDLSKTAQICLVSSHNEVYEPCDDSFALVDALLADRTNLLEHHLALCMEIGCGSGYVITSLALILGQEGSGVNYIATDINPHAVKVTRETMEAHGVDAELIITDIASGLENRLAGMIDVMVVNPPYVPTPEAEVGSEGITASWAGGENGRSVIDRILPVADALLSEKGWLYMVTLTANNPSEICLEMRKKGYASKIVVQRSTEEESLHIIKFWRDLDNKLDENDQSTSGFMGSLLSQIPLLSYWRGNNSDGKC*