Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88171.t1 | XP_024636344.1 | 88 | 882 | 86 | 4 | 1 | 877 | 1 | 867 | 0.00E+00 | 1562.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88171.t1 | Q9FQ03 | 46.0 | 842 | 329 | 15 | 1 | 734 | 1 | 824 | 5.8e-190 | 666.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88171.t1 | G7JH90 | 86.8 | 879 | 81 | 4 | 1 | 877 | 1 | 846 | 0.0e+00 | 1530.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene88171 | 0.868336 | 7.04E-66 | -1.69E-46 |
| MS.gene049159 | MS.gene88171 | 0.812382 | 4.36E-51 | -1.69E-46 |
| MS.gene049190 | MS.gene88171 | 0.804455 | 2.14E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene88171 | 0.88792 | 9.34E-73 | -1.69E-46 |
| MS.gene049272 | MS.gene88171 | 0.817754 | 2.80E-52 | -1.69E-46 |
| MS.gene049343 | MS.gene88171 | 0.82323 | 1.55E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene88171 | 0.8387 | 2.46E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene88171 | 0.828092 | 1.09E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene88171 | 0.828804 | 7.33E-55 | -1.69E-46 |
| MS.gene049567 | MS.gene88171 | 0.8119 | 5.56E-51 | -1.69E-46 |
| MS.gene049585 | MS.gene88171 | 0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene049672 | MS.gene88171 | 0.803579 | 3.26E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene88171 | 0.870236 | 1.70E-66 | -1.69E-46 |
| MS.gene049807 | MS.gene88171 | 0.813433 | 2.57E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene88171 | 0.810534 | 1.10E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene88171 | 0.821138 | 4.73E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene88171 | 0.855042 | 8.23E-62 | -1.69E-46 |
| MS.gene049873 | MS.gene88171 | 0.81858 | 1.82E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene88171 | 0.812969 | 3.24E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene88171 | 0.862369 | 5.32E-64 | -1.69E-46 |
| MS.gene05016 | MS.gene88171 | 0.80406 | 2.59E-49 | -1.69E-46 |
| MS.gene050221 | MS.gene88171 | 0.83239 | 9.71E-56 | -1.69E-46 |
| MS.gene050273 | MS.gene88171 | 0.810586 | 1.07E-50 | -1.69E-46 |
| MS.gene050287 | MS.gene88171 | -0.811207 | 7.86E-51 | -1.69E-46 |
| MS.gene050355 | MS.gene88171 | 0.818838 | 1.59E-52 | -1.69E-46 |
| MS.gene05045 | MS.gene88171 | 0.826872 | 2.14E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene88171 | 0.859657 | 3.55E-63 | -1.69E-46 |
| MS.gene050561 | MS.gene88171 | 0.861509 | 9.76E-64 | -1.69E-46 |
| MS.gene050562 | MS.gene88171 | 0.848082 | 7.69E-60 | -1.69E-46 |
| MS.gene050657 | MS.gene88171 | 0.806317 | 8.72E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene88171 | 0.83901 | 2.05E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene88171 | 0.804639 | 1.96E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene88171 | 0.801573 | 8.45E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene88171 | 0.811673 | 6.22E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene88171 | 0.850414 | 1.72E-60 | -1.69E-46 |
| MS.gene051279 | MS.gene88171 | 0.85496 | 8.69E-62 | -1.69E-46 |
| MS.gene051332 | MS.gene88171 | 0.816312 | 5.90E-52 | -1.69E-46 |
| MS.gene051388 | MS.gene88171 | 0.816931 | 4.29E-52 | -1.69E-46 |
| MS.gene051594 | MS.gene88171 | 0.819459 | 1.15E-52 | -1.69E-46 |
| MS.gene051700 | MS.gene88171 | 0.80317 | 3.96E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene88171 | 0.826102 | 3.26E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene88171 | 0.839145 | 1.89E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene88171 | 0.890955 | 6.17E-74 | -1.69E-46 |
| MS.gene051800 | MS.gene88171 | 0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene051957 | MS.gene88171 | 0.837326 | 5.55E-57 | -1.69E-46 |
| MS.gene052020 | MS.gene88171 | 0.812302 | 4.54E-51 | -1.69E-46 |
| MS.gene052037 | MS.gene88171 | 0.831944 | 1.25E-55 | -1.69E-46 |
| MS.gene052208 | MS.gene88171 | 0.813166 | 2.94E-51 | -1.69E-46 |
| MS.gene052239 | MS.gene88171 | -0.8085 | 3.00E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene88171 | 0.803477 | 3.42E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene88171 | 0.818556 | 1.84E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene88171 | 0.856911 | 2.33E-62 | -1.69E-46 |
| MS.gene052519 | MS.gene88171 | 0.855686 | 5.34E-62 | -1.69E-46 |
| MS.gene052534 | MS.gene88171 | 0.811857 | 5.68E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene88171 | 0.82978 | 4.25E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene88171 | 0.847355 | 1.22E-59 | -1.69E-46 |
| MS.gene052611 | MS.gene88171 | 0.8645 | 1.16E-64 | -1.69E-46 |
| MS.gene052773 | MS.gene88171 | 0.850051 | 2.18E-60 | -1.69E-46 |
| MS.gene052863 | MS.gene88171 | 0.852977 | 3.24E-61 | -1.69E-46 |
| MS.gene052896 | MS.gene88171 | 0.811759 | 5.96E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene88171 | 0.800261 | 1.57E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene88171 | 0.804348 | 2.26E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene88171 | 0.840253 | 9.72E-58 | -1.69E-46 |
| MS.gene053145 | MS.gene88171 | 0.800405 | 1.46E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene88171 | 0.805898 | 1.07E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene88171 | 0.890148 | 1.28E-73 | -1.69E-46 |
| MS.gene053296 | MS.gene88171 | 0.808528 | 2.96E-50 | -1.69E-46 |
| MS.gene053599 | MS.gene88171 | 0.805527 | 1.28E-49 | -1.69E-46 |
| MS.gene053621 | MS.gene88171 | 0.804366 | 2.24E-49 | -1.69E-46 |
| MS.gene053647 | MS.gene88171 | 0.807167 | 5.77E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene88171 | 0.803502 | 3.38E-49 | -1.69E-46 |
| MS.gene053900 | MS.gene88171 | 0.806336 | 8.64E-50 | -1.69E-46 |
| MS.gene053966 | MS.gene88171 | 0.805337 | 1.40E-49 | -1.69E-46 |
| MS.gene053998 | MS.gene88171 | 0.853181 | 2.83E-61 | -1.69E-46 |
| MS.gene05414 | MS.gene88171 | 0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene88171 | 0.827369 | 1.63E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene88171 | 0.82027 | 7.50E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene88171 | 0.820221 | 7.69E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene88171 | 0.831184 | 1.93E-55 | -1.69E-46 |
| MS.gene054472 | MS.gene88171 | 0.809934 | 1.48E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene88171 | 0.816434 | 5.54E-52 | -1.69E-46 |
| MS.gene054582 | MS.gene88171 | 0.804341 | 2.26E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene88171 | 0.833156 | 6.27E-56 | -1.69E-46 |
| MS.gene05458 | MS.gene88171 | 0.815953 | 7.10E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene88171 | 0.868217 | 7.69E-66 | -1.69E-46 |
| MS.gene05468 | MS.gene88171 | 0.8102 | 1.30E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene88171 | 0.836393 | 9.61E-57 | -1.69E-46 |
| MS.gene054991 | MS.gene88171 | 0.825976 | 3.49E-54 | -1.69E-46 |
| MS.gene055083 | MS.gene88171 | 0.822449 | 2.36E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene88171 | 0.845959 | 2.93E-59 | -1.69E-46 |
| MS.gene055228 | MS.gene88171 | 0.817608 | 3.02E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene88171 | 0.806559 | 7.76E-50 | -1.69E-46 |
| MS.gene055464 | MS.gene88171 | 0.82021 | 7.74E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene88171 | 0.830066 | 3.62E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene88171 | 0.841625 | 4.24E-58 | -1.69E-46 |
| MS.gene055468 | MS.gene88171 | 0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene88171 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene88171 | 0.814588 | 1.43E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene88171 | 0.805105 | 1.57E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene88171 | -0.811972 | 5.36E-51 | -1.69E-46 |
| MS.gene055586 | MS.gene88171 | 0.826043 | 3.37E-54 | -1.69E-46 |
| MS.gene055589 | MS.gene88171 | 0.833258 | 5.91E-56 | -1.69E-46 |
| MS.gene055778 | MS.gene88171 | 0.842311 | 2.79E-58 | -1.69E-46 |
| MS.gene055818 | MS.gene88171 | 0.883742 | 3.47E-71 | -1.69E-46 |
| MS.gene055926 | MS.gene88171 | 0.803776 | 2.97E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene88171 | 0.835286 | 1.83E-56 | -1.69E-46 |
| MS.gene056050 | MS.gene88171 | 0.869527 | 2.90E-66 | -1.69E-46 |
| MS.gene056173 | MS.gene88171 | 0.810856 | 9.36E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene88171 | 0.833259 | 5.91E-56 | -1.69E-46 |
| MS.gene056252 | MS.gene88171 | 0.809472 | 1.86E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene88171 | 0.808851 | 2.53E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene88171 | 0.854415 | 1.25E-61 | -1.69E-46 |
| MS.gene056373 | MS.gene88171 | 0.810855 | 9.37E-51 | -1.69E-46 |
| MS.gene056424 | MS.gene88171 | 0.819333 | 1.23E-52 | -1.69E-46 |
| MS.gene056507 | MS.gene88171 | 0.80756 | 4.76E-50 | -1.69E-46 |
| MS.gene056542 | MS.gene88171 | 0.806185 | 9.30E-50 | -1.69E-46 |
| MS.gene056543 | MS.gene88171 | 0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene056545 | MS.gene88171 | 0.809164 | 2.17E-50 | -1.69E-46 |
| MS.gene056553 | MS.gene88171 | 0.840301 | 9.44E-58 | -1.69E-46 |
| MS.gene056595 | MS.gene88171 | 0.847567 | 1.07E-59 | -1.69E-46 |
| MS.gene05659 | MS.gene88171 | 0.802422 | 5.65E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene88171 | 0.813115 | 3.01E-51 | -1.69E-46 |
| MS.gene056854 | MS.gene88171 | 0.825972 | 3.50E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene88171 | 0.873791 | 1.12E-67 | -1.69E-46 |
| MS.gene05690 | MS.gene88171 | 0.821267 | 4.42E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene88171 | 0.845487 | 3.94E-59 | -1.69E-46 |
| MS.gene057034 | MS.gene88171 | 0.800328 | 1.52E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene88171 | 0.815161 | 1.07E-51 | -1.69E-46 |
| MS.gene057132 | MS.gene88171 | 0.83364 | 4.75E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene88171 | 0.816997 | 4.14E-52 | -1.69E-46 |
| MS.gene057314 | MS.gene88171 | 0.820039 | 8.47E-53 | -1.69E-46 |
| MS.gene057324 | MS.gene88171 | 0.80251 | 5.42E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene88171 | 0.82575 | 3.95E-54 | -1.69E-46 |
| MS.gene057598 | MS.gene88171 | 0.80538 | 1.37E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene88171 | 0.818065 | 2.38E-52 | -1.69E-46 |
| MS.gene057855 | MS.gene88171 | 0.814066 | 1.86E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene88171 | 0.849278 | 3.58E-60 | -1.69E-46 |
| MS.gene057921 | MS.gene88171 | 0.80617 | 9.37E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene88171 | 0.823335 | 1.46E-53 | -1.69E-46 |
| MS.gene058010 | MS.gene88171 | 0.851955 | 6.33E-61 | -1.69E-46 |
| MS.gene058037 | MS.gene88171 | 0.831783 | 1.37E-55 | -1.69E-46 |
| MS.gene058339 | MS.gene88171 | 0.80862 | 2.83E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene88171 | 0.817664 | 2.93E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene88171 | 0.87914 | 1.59E-69 | -1.69E-46 |
| MS.gene058668 | MS.gene88171 | 0.804856 | 1.77E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene88171 | 0.830462 | 2.90E-55 | -1.69E-46 |
| MS.gene058829 | MS.gene88171 | 0.842752 | 2.13E-58 | -1.69E-46 |
| MS.gene058979 | MS.gene88171 | 0.85115 | 1.07E-60 | -1.69E-46 |
| MS.gene05897 | MS.gene88171 | 0.813734 | 2.20E-51 | -1.69E-46 |
| MS.gene059015 | MS.gene88171 | 0.837499 | 5.02E-57 | -1.69E-46 |
| MS.gene059272 | MS.gene88171 | 0.86874 | 5.22E-66 | -1.69E-46 |
| MS.gene059439 | MS.gene88171 | 0.825017 | 5.90E-54 | -1.69E-46 |
| MS.gene059570 | MS.gene88171 | 0.831381 | 1.72E-55 | -1.69E-46 |
| MS.gene059584 | MS.gene88171 | 0.808215 | 3.46E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene88171 | 0.833792 | 4.35E-56 | -1.69E-46 |
| MS.gene059725 | MS.gene88171 | 0.811378 | 7.21E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene88171 | 0.814853 | 1.25E-51 | -1.69E-46 |
| MS.gene060167 | MS.gene88171 | 0.820984 | 5.14E-53 | -1.69E-46 |
| MS.gene060312 | MS.gene88171 | 0.838826 | 2.28E-57 | -1.69E-46 |
| MS.gene060322 | MS.gene88171 | 0.839462 | 1.56E-57 | -1.69E-46 |
| MS.gene060365 | MS.gene88171 | 0.804582 | 2.02E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene88171 | 0.818786 | 1.63E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene88171 | 0.802871 | 4.57E-49 | -1.69E-46 |
| MS.gene060554 | MS.gene88171 | 0.800716 | 1.26E-48 | -1.69E-46 |
| MS.gene060587 | MS.gene88171 | 0.809288 | 2.04E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene88171 | 0.808937 | 2.42E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene88171 | 0.80152 | 8.66E-49 | -1.69E-46 |
| MS.gene06094 | MS.gene88171 | 0.803231 | 3.85E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene88171 | 0.820307 | 7.35E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88171.t1 | MTR_4g051920 | 87.372 | 879 | 76 | 4 | 1 | 877 | 1 | 846 | 0.0 | 1567 |
| MS.gene88171.t1 | MTR_7g114570 | 39.233 | 808 | 325 | 19 | 1 | 732 | 1 | 718 | 5.48e-163 | 500 |
| MS.gene88171.t1 | MTR_7g114570 | 39.088 | 811 | 333 | 20 | 1 | 732 | 1 | 729 | 8.54e-163 | 499 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88171.t1 | AT1G75660 | 46.310 | 840 | 325 | 16 | 1 | 732 | 1 | 822 | 0.0 | 659 |
| MS.gene88171.t1 | AT5G42540 | 45.618 | 833 | 335 | 15 | 1 | 732 | 1 | 816 | 0.0 | 642 |
| MS.gene88171.t1 | AT5G42540 | 45.024 | 844 | 335 | 16 | 1 | 732 | 1 | 827 | 0.0 | 634 |
| MS.gene88171.t1 | AT1G54490 | 43.171 | 820 | 312 | 15 | 1 | 746 | 1 | 740 | 0.0 | 617 |
| MS.gene88171.t1 | AT1G54490 | 43.048 | 748 | 293 | 13 | 56 | 746 | 11 | 682 | 0.0 | 560 |
Find 146 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACACGAATTTCATCGTATT+TGG | 0.150872 | 4.4:+50508292 | None:intergenic |
| TCCATTTGCTTCGGACTTAA+AGG | 0.176185 | 4.4:-50505278 | MS.gene88171:CDS |
| AACACGGTTCACTGTTTATA+TGG | 0.203040 | 4.4:-50507743 | MS.gene88171:CDS |
| AGTCAGCATTATAATCGTTT+TGG | 0.237014 | 4.4:-50503889 | MS.gene88171:CDS |
| GCTCCTTTGTGAATTGATTC+TGG | 0.243447 | 4.4:+50503533 | None:intergenic |
| ATCTTCATTTGTTTCTTTGC+TGG | 0.261003 | 4.4:-50506963 | MS.gene88171:CDS |
| CCACCAACATTGAGCTAAAA+AGG | 0.271417 | 4.4:-50505835 | MS.gene88171:CDS |
| TCTGCCTTACTTGCCATCTT+TGG | 0.273671 | 4.4:-50506931 | MS.gene88171:CDS |
| GCCTCGCAAGCTATTGTATA+TGG | 0.302733 | 4.4:-50509949 | MS.gene88171:CDS |
| GCTGGTATTGGATGGGGTTC+AGG | 0.308029 | 4.4:-50503512 | MS.gene88171:CDS |
| CCACCAAAACTAGTTGAGTT+AGG | 0.311068 | 4.4:+50503581 | None:intergenic |
| TTATCTGATGCTAATGTTCC+TGG | 0.318479 | 4.4:-50507830 | MS.gene88171:CDS |
| ATCAATTGTGCATAGGCTTT+AGG | 0.320131 | 4.4:+50505059 | None:intergenic |
| GGATATCCTCTGATCCATTA+TGG | 0.339612 | 4.4:-50508006 | MS.gene88171:CDS |
| TTGTTGTTGCTATCATCATT+AGG | 0.343938 | 4.4:+50510138 | None:intergenic |
| CGAATTCCAACAGCTCATTT+AGG | 0.348865 | 4.4:-50503295 | MS.gene88171:CDS |
| GACACTTCACATTCTTGTTT+AGG | 0.353004 | 4.4:+50508106 | None:intergenic |
| CATTTGAAGTGTTTGTGTTA+AGG | 0.354839 | 4.4:+50510217 | None:intergenic |
| TGTATGCAAACTTCCATATA+TGG | 0.356068 | 4.4:-50504745 | MS.gene88171:CDS |
| CAACGTTCGCATGTGCCTTT+TGG | 0.361980 | 4.4:-50503380 | MS.gene88171:CDS |
| TTGAACCGCATTGGAGCATT+TGG | 0.362324 | 4.4:+50507176 | None:intergenic |
| GAAATTCAGTTGAAGTTGAT+AGG | 0.363011 | 4.4:-50504568 | MS.gene88171:CDS |
| GAAGGTTTGCACCAGATAAT+AGG | 0.365368 | 4.4:+50504132 | None:intergenic |
| TTAATGTCTTCAAACACCTT+TGG | 0.379530 | 4.4:+50510240 | None:intergenic |
| TTCCATGCATACACATTGTT+CGG | 0.382556 | 4.4:+50503329 | None:intergenic |
| ACCATAACACCCTTCCATAA+TGG | 0.383134 | 4.4:+50507992 | None:intergenic |
| CCATTTGCTTCGGACTTAAA+GGG | 0.386749 | 4.4:-50505277 | MS.gene88171:CDS |
| TAAGATCCTCCATGATCATT+AGG | 0.398845 | 4.4:+50503413 | None:intergenic |
| TTAAGAGTTATATTTCTTCA+AGG | 0.402191 | 4.4:-50508027 | MS.gene88171:CDS |
| AAGCTATTGTATATGGCTAT+TGG | 0.413300 | 4.4:-50509942 | MS.gene88171:intron |
| TGAGATAGAAATGAAGATAC+AGG | 0.417312 | 4.4:-50507036 | MS.gene88171:CDS |
| CACCAAAACTAGTTGAGTTA+GGG | 0.419048 | 4.4:+50503582 | None:intergenic |
| TAAACCGTCTAGTCCTTTGC+TGG | 0.424583 | 4.4:+50508321 | None:intergenic |
| CTTCCTCAGTCTCTCTTCCT+CGG | 0.425369 | 4.4:+50508159 | None:intergenic |
| TAGATAAAATTAGGAACTGT+TGG | 0.427627 | 4.4:-50505902 | MS.gene88171:CDS |
| ACTGAGGAAGGAATTTGAAA+TGG | 0.428253 | 4.4:-50508146 | MS.gene88171:CDS |
| TCCATTATGGAAGGGTGTTA+TGG | 0.432134 | 4.4:-50507993 | MS.gene88171:intron |
| TGCTCCTTCAACAACATTTG+TGG | 0.434639 | 4.4:-50510024 | MS.gene88171:CDS |
| GGTTCACTGTTTATATGGAT+TGG | 0.435103 | 4.4:-50507738 | MS.gene88171:intron |
| CATAAACAACCTAATGATCA+TGG | 0.436115 | 4.4:-50503422 | MS.gene88171:CDS |
| ATTCACAAAGGAGCTGGTAT+TGG | 0.439239 | 4.4:-50503524 | MS.gene88171:CDS |
| CATGTTCGGCACCTATTATC+TGG | 0.440662 | 4.4:-50504143 | MS.gene88171:CDS |
| ATTGGATGGGGTTCAGGTAG+AGG | 0.441242 | 4.4:-50503506 | MS.gene88171:CDS |
| AAATGCTCCAATGCGGTTCA+AGG | 0.446020 | 4.4:-50507174 | MS.gene88171:intron |
| CCCTTTAAGTCCGAAGCAAA+TGG | 0.454665 | 4.4:+50505277 | None:intergenic |
| AGTTGAAGTTGATAGGTTGT+TGG | 0.457586 | 4.4:-50504561 | MS.gene88171:CDS |
| TGCTTCGGACTTAAAGGGCA+TGG | 0.458359 | 4.4:-50505272 | MS.gene88171:CDS |
| CCACTATGGTCCATTTGCTT+CGG | 0.459238 | 4.4:-50505287 | MS.gene88171:CDS |
| TCATTAGGTTGTTTATGAAA+AGG | 0.459890 | 4.4:+50503428 | None:intergenic |
| TTTGCATATTTGGTTGTTGA+GGG | 0.461938 | 4.4:-50507066 | MS.gene88171:CDS |
| TATAAACAGTGAACCGTGTT+CGG | 0.463074 | 4.4:+50507746 | None:intergenic |
| AGTTGATCAAAAGGTAAGAA+TGG | 0.463553 | 4.4:+50505217 | None:intergenic |
| ACCGTGTTCGGATCATAATC+AGG | 0.465301 | 4.4:+50507758 | None:intergenic |
| ACACTTAAAAGTTGATCAAA+AGG | 0.465449 | 4.4:+50505208 | None:intergenic |
| TGCAGCTTCTCATTATAGTC+AGG | 0.471855 | 4.4:+50507200 | None:intergenic |
| ACTTCCACAAATGTTGTTGA+AGG | 0.478416 | 4.4:+50510020 | None:intergenic |
| ATATCCAAAGATGGCAAGTA+AGG | 0.478852 | 4.4:+50506927 | None:intergenic |
| TAAGTGTTCTCCCTCAAAAG+AGG | 0.480997 | 4.4:-50505192 | MS.gene88171:intron |
| ATAAAATTATGTCATTCATA+AGG | 0.481208 | 4.4:-50507796 | MS.gene88171:CDS |
| TAATCCATTCTTGAGTTCGC+TGG | 0.481878 | 4.4:+50504698 | None:intergenic |
| AATTAAACCTTGAACCGCAT+TGG | 0.483273 | 4.4:+50507167 | None:intergenic |
| GTCAAATACGAGCATCCTAC+TGG | 0.486901 | 4.4:-50504176 | MS.gene88171:CDS |
| CAGGAGTTGCTTCATGGACA+TGG | 0.488947 | 4.4:-50505563 | MS.gene88171:intron |
| ACAGCTCATTTAGGCCTCAA+TGG | 0.490917 | 4.4:-50503286 | MS.gene88171:CDS |
| CAAAGGAGCTGGTATTGGAT+GGG | 0.513467 | 4.4:-50503519 | MS.gene88171:CDS |
| AGGAAAGCGCTTCATGTGGC+AGG | 0.513892 | 4.4:-50504896 | MS.gene88171:intron |
| GATGCTGATCTTATAATGCT+TGG | 0.516302 | 4.4:-50507628 | MS.gene88171:CDS |
| TGTTGCTATCATCATTAGGA+TGG | 0.516700 | 4.4:+50510142 | None:intergenic |
| GCTTCGGACTTAAAGGGCAT+GGG | 0.518549 | 4.4:-50505271 | MS.gene88171:CDS |
| TTTAGCAGCGACGGCATTGG+AGG | 0.518763 | 4.4:-50504344 | MS.gene88171:intron |
| AGCAAATGCATAAAAGAGAA+AGG | 0.519730 | 4.4:-50506124 | MS.gene88171:CDS |
| ATGCTAATGTTCCTGGAGAA+GGG | 0.520161 | 4.4:-50507823 | MS.gene88171:CDS |
| TTGAGGCCTAAATGAGCTGT+TGG | 0.520881 | 4.4:+50503289 | None:intergenic |
| TGCTGCATGAATTCTGTCCC+TGG | 0.521072 | 4.4:+50508064 | None:intergenic |
| GAAACCAGCGAACTCAAGAA+TGG | 0.521588 | 4.4:-50504702 | MS.gene88171:CDS |
| GATGCTAATGTTCCTGGAGA+AGG | 0.521838 | 4.4:-50507824 | MS.gene88171:CDS |
| ACCTCGTGAATATCCAAAGA+TGG | 0.523973 | 4.4:+50506918 | None:intergenic |
| AGCCTATGCACAATTGATGT+TGG | 0.525564 | 4.4:-50505054 | MS.gene88171:CDS |
| TCCTTTGAATCACTCCATTG+AGG | 0.527231 | 4.4:+50503272 | None:intergenic |
| TTGGATGGGGTTCAGGTAGA+GGG | 0.528433 | 4.4:-50503505 | MS.gene88171:CDS |
| ACAAAGGAGCTGGTATTGGA+TGG | 0.528593 | 4.4:-50503520 | MS.gene88171:CDS |
| ACAAGGTGGACACAAGCATA+AGG | 0.533115 | 4.4:-50504490 | MS.gene88171:intron |
| AAAGGCGACTTGTTTAAAAG+TGG | 0.535755 | 4.4:-50506106 | MS.gene88171:CDS |
| CTACCTCGTCCTCTACCAAA+AGG | 0.537396 | 4.4:+50503365 | None:intergenic |
| GTACACTGAAGGATTGTTGT+GGG | 0.539897 | 4.4:-50505604 | MS.gene88171:CDS |
| GAATCAATTCACAAAGGAGC+TGG | 0.539955 | 4.4:-50503530 | MS.gene88171:CDS |
| ACAGAATGCATGTGATTACA+TGG | 0.542418 | 4.4:-50506369 | MS.gene88171:CDS |
| AGGAAGGAATTTGAAATGGA+AGG | 0.549315 | 4.4:-50508142 | MS.gene88171:CDS |
| ACCAAAACTAGTTGAGTTAG+GGG | 0.549416 | 4.4:+50503583 | None:intergenic |
| ATCCTCTGATCCATTATGGA+AGG | 0.552359 | 4.4:-50508002 | MS.gene88171:CDS |
| ACTTCGTCTTTGATCTTGAG+CGG | 0.552614 | 4.4:-50507001 | MS.gene88171:CDS |
| GCCTCAATGGAGTGATTCAA+AGG | 0.557141 | 4.4:-50503273 | MS.gene88171:CDS |
| TGGTGCAAACCTTCCAGAGA+AGG | 0.557181 | 4.4:-50504123 | MS.gene88171:intron |
| TAACACAAACACTTCAAATG+TGG | 0.557233 | 4.4:-50510214 | MS.gene88171:CDS |
| ATCCGAACAATGTGTATGCA+TGG | 0.558248 | 4.4:-50503331 | MS.gene88171:CDS |
| CTCACTTAAATTAAACAAGG+TGG | 0.569573 | 4.4:-50504504 | MS.gene88171:CDS |
| CTGGTGGTACAAGTCATGTT+CGG | 0.570286 | 4.4:-50504157 | MS.gene88171:CDS |
| CAGGTAGAGGGAAATCAGAA+AGG | 0.576545 | 4.4:-50503493 | MS.gene88171:CDS |
| AAAGGAGCTGGTATTGGATG+GGG | 0.579160 | 4.4:-50503518 | MS.gene88171:CDS |
| CCTTTGTGAATTGATTCTGG+AGG | 0.581542 | 4.4:+50503536 | None:intergenic |
| TGCTAATGTTCCTGGAGAAG+GGG | 0.588627 | 4.4:-50507822 | MS.gene88171:CDS |
| GCCATATACAATAGCTTGCG+AGG | 0.591780 | 4.4:+50509948 | None:intergenic |
| ATTCAAAGGATAAAAGTCGT+TGG | 0.593054 | 4.4:-50503259 | MS.gene88171:CDS |
| AGACTGAGGACAAACATTGT+CGG | 0.594878 | 4.4:+50506289 | None:intergenic |
| GGTCAAATAATCAAGACACT+TGG | 0.597825 | 4.4:-50503643 | MS.gene88171:CDS |
| GTTGATAGGTTGTTGGTCAG+CGG | 0.602504 | 4.4:-50504554 | MS.gene88171:CDS |
| GATTCAAATATTATAACACC+AGG | 0.602504 | 4.4:-50508082 | MS.gene88171:CDS |
| AGTACACTGAAGGATTGTTG+TGG | 0.604142 | 4.4:-50505605 | MS.gene88171:CDS |
| TTAGCAGCGACGGCATTGGA+GGG | 0.605881 | 4.4:-50504343 | MS.gene88171:intron |
| GCAAATGGACCATAGTGGTA+GGG | 0.606211 | 4.4:+50505292 | None:intergenic |
| TGTTGTTCTCAACACACTCG+TGG | 0.606281 | 4.4:+50504306 | None:intergenic |
| TCCTCTGATCCATTATGGAA+GGG | 0.606739 | 4.4:-50508001 | MS.gene88171:CDS |
| TAAACTTGGAGAATATCTTG+TGG | 0.609289 | 4.4:-50506764 | MS.gene88171:CDS |
| ATTCATGCAGCAACTTTCGA+AGG | 0.609533 | 4.4:-50508053 | MS.gene88171:CDS |
| CGAGGAAGAGAGACTGAGGA+AGG | 0.609831 | 4.4:-50508158 | MS.gene88171:CDS |
| ATCCAACATCAATTGTGCAT+AGG | 0.611572 | 4.4:+50505052 | None:intergenic |
| GAGCGAAAATGAACCAGCAA+AGG | 0.612334 | 4.4:-50508334 | MS.gene88171:CDS |
| ACATAAATGAGACGATATTG+TGG | 0.612573 | 4.4:-50503921 | MS.gene88171:CDS |
| ACCCTTCCATAATGGATCAG+AGG | 0.615223 | 4.4:+50508000 | None:intergenic |
| TGGATGAATTCAAACAGAGA+AGG | 0.615569 | 4.4:-50503623 | MS.gene88171:CDS |
| TTGCTCACTTAAATTAAACA+AGG | 0.615653 | 4.4:-50504507 | MS.gene88171:CDS |
| AGCAAATGGACCATAGTGGT+AGG | 0.618914 | 4.4:+50505291 | None:intergenic |
| CCGAAGCAAATGGACCATAG+TGG | 0.629001 | 4.4:+50505287 | None:intergenic |
| TCCTGATTATGATCCGAACA+CGG | 0.629375 | 4.4:-50507759 | MS.gene88171:CDS |
| GTTACTCAACAAATATCCAA+AGG | 0.635055 | 4.4:-50510256 | MS.gene88171:CDS |
| TGAACCAGCAAAGGACTAGA+CGG | 0.636674 | 4.4:-50508325 | MS.gene88171:CDS |
| ACATGACTTGTACCACCAGT+AGG | 0.639002 | 4.4:+50504161 | None:intergenic |
| GCCATCTTTGGATATTCACG+AGG | 0.647327 | 4.4:-50506919 | MS.gene88171:intron |
| TAGGTTCAGAAGTACACTGA+AGG | 0.653526 | 4.4:-50505615 | MS.gene88171:CDS |
| TCATCATTAGGATGGAAACA+AGG | 0.654793 | 4.4:+50510150 | None:intergenic |
| AGTAACCATCTATAAAACGA+TGG | 0.659634 | 4.4:+50510273 | None:intergenic |
| TTTGTATTTATAGGAAGCCG+AGG | 0.659675 | 4.4:-50508176 | MS.gene88171:intron |
| ATATCTTGTGGACATAAACA+AGG | 0.669996 | 4.4:-50506752 | MS.gene88171:intron |
| TAGAAGGAAAGCGCTTCATG+TGG | 0.670368 | 4.4:-50504900 | MS.gene88171:CDS |
| AAGCCGAGGAAGAGAGACTG+AGG | 0.671391 | 4.4:-50508162 | MS.gene88171:CDS |
| CTTACTTCTTCATCAGACTG+AGG | 0.677507 | 4.4:+50506275 | None:intergenic |
| AAACAGTTAGGGGAGAAACA+TGG | 0.682040 | 4.4:-50506614 | MS.gene88171:intron |
| CCAAAACTAGTTGAGTTAGG+GGG | 0.684296 | 4.4:+50503584 | None:intergenic |
| CAAAAGGCACATGCGAACGT+TGG | 0.684654 | 4.4:+50503381 | None:intergenic |
| CCTCCAGAATCAATTCACAA+AGG | 0.701480 | 4.4:-50503536 | MS.gene88171:CDS |
| TGGAGATTACTTGACTGACA+AGG | 0.707640 | 4.4:-50506086 | MS.gene88171:intron |
| TGCAACCAAATGCTCCAATG+CGG | 0.710410 | 4.4:-50507181 | MS.gene88171:CDS |
| AAATACGAGCATCCTACTGG+TGG | 0.711420 | 4.4:-50504173 | MS.gene88171:CDS |
| ATTCAAATATTATAACACCA+GGG | 0.720952 | 4.4:-50508081 | MS.gene88171:CDS |
| AAACAACCTAATGATCATGG+AGG | 0.725320 | 4.4:-50503419 | MS.gene88171:CDS |
| TCATTCATAAGGAAACAGCG+TGG | 0.793260 | 4.4:-50507785 | MS.gene88171:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 50503256 | 50510304 | 50503256 | ID=MS.gene88171 |
| chr4.4 | mRNA | 50503256 | 50510304 | 50503256 | ID=MS.gene88171.t1;Parent=MS.gene88171 |
| chr4.4 | exon | 50510137 | 50510304 | 50510137 | ID=MS.gene88171.t1.exon1;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50510137 | 50510304 | 50510137 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50509943 | 50510048 | 50509943 | ID=MS.gene88171.t1.exon2;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50509943 | 50510048 | 50509943 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50508295 | 50508371 | 50508295 | ID=MS.gene88171.t1.exon3;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50508295 | 50508371 | 50508295 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50507994 | 50508185 | 50507994 | ID=MS.gene88171.t1.exon4;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50507994 | 50508185 | 50507994 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50507739 | 50507858 | 50507739 | ID=MS.gene88171.t1.exon5;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50507739 | 50507858 | 50507739 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50507588 | 50507650 | 50507588 | ID=MS.gene88171.t1.exon6;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50507588 | 50507650 | 50507588 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50507175 | 50507228 | 50507175 | ID=MS.gene88171.t1.exon7;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50507175 | 50507228 | 50507175 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50506920 | 50507090 | 50506920 | ID=MS.gene88171.t1.exon8;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50506920 | 50507090 | 50506920 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50506753 | 50506830 | 50506753 | ID=MS.gene88171.t1.exon9;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50506753 | 50506830 | 50506753 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50506481 | 50506630 | 50506481 | ID=MS.gene88171.t1.exon10;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50506481 | 50506630 | 50506481 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50506280 | 50506399 | 50506280 | ID=MS.gene88171.t1.exon11;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50506280 | 50506399 | 50506280 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50506087 | 50506182 | 50506087 | ID=MS.gene88171.t1.exon12;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50506087 | 50506182 | 50506087 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50505826 | 50505921 | 50505826 | ID=MS.gene88171.t1.exon13;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50505826 | 50505921 | 50505826 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50505564 | 50505634 | 50505564 | ID=MS.gene88171.t1.exon14;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50505564 | 50505634 | 50505564 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50505193 | 50505321 | 50505193 | ID=MS.gene88171.t1.exon15;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50505193 | 50505321 | 50505193 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50505018 | 50505094 | 50505018 | ID=MS.gene88171.t1.exon16;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50505018 | 50505094 | 50505018 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50504897 | 50504937 | 50504897 | ID=MS.gene88171.t1.exon17;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50504897 | 50504937 | 50504897 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50504695 | 50504769 | 50504695 | ID=MS.gene88171.t1.exon18;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50504695 | 50504769 | 50504695 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50504491 | 50504606 | 50504491 | ID=MS.gene88171.t1.exon19;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50504491 | 50504606 | 50504491 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50504287 | 50504358 | 50504287 | ID=MS.gene88171.t1.exon20;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50504287 | 50504358 | 50504287 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50504124 | 50504202 | 50504124 | ID=MS.gene88171.t1.exon21;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50504124 | 50504202 | 50504124 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50503886 | 50503959 | 50503886 | ID=MS.gene88171.t1.exon22;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50503886 | 50503959 | 50503886 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| chr4.4 | exon | 50503256 | 50503664 | 50503256 | ID=MS.gene88171.t1.exon23;Parent=MS.gene88171.t1 |
| chr4.4 | CDS | 50503256 | 50503664 | 50503256 | ID=cds.MS.gene88171.t1;Parent=MS.gene88171.t1 |
| Gene Sequence |
| Protein sequence |
