Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58165.t1 | XP_003630120.1 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.90E-87 | 331.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58165.t1 | Q9XIK3 | 65.4 | 185 | 46 | 4 | 7 | 179 | 2 | 180 | 7.7e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58165.t1 | G7LBB6 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.1e-87 | 331.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene58165 | 0.869039 | 4.17E-66 | -1.69E-46 |
| MS.gene049222 | MS.gene58165 | 0.868349 | 6.98E-66 | -1.69E-46 |
| MS.gene049232 | MS.gene58165 | 0.820663 | 6.09E-53 | -1.69E-46 |
| MS.gene049251 | MS.gene58165 | -0.800941 | 1.14E-48 | -1.69E-46 |
| MS.gene049285 | MS.gene58165 | 0.833788 | 4.36E-56 | -1.69E-46 |
| MS.gene049320 | MS.gene58165 | 0.831897 | 1.29E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene58165 | 0.835443 | 1.67E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene58165 | 0.884075 | 2.61E-71 | -1.69E-46 |
| MS.gene05010 | MS.gene58165 | 0.816409 | 5.61E-52 | -1.69E-46 |
| MS.gene050170 | MS.gene58165 | 0.909192 | 7.47E-82 | -1.69E-46 |
| MS.gene050323 | MS.gene58165 | 0.871923 | 4.72E-67 | -1.69E-46 |
| MS.gene050338 | MS.gene58165 | -0.837324 | 5.56E-57 | -1.69E-46 |
| MS.gene050444 | MS.gene58165 | 0.825156 | 5.47E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene58165 | 0.869161 | 3.81E-66 | -1.69E-46 |
| MS.gene050502 | MS.gene58165 | 0.807995 | 3.85E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene58165 | 0.852945 | 3.31E-61 | -1.69E-46 |
| MS.gene050518 | MS.gene58165 | 0.82767 | 1.38E-54 | -1.69E-46 |
| MS.gene050538 | MS.gene58165 | 0.81847 | 1.93E-52 | -1.69E-46 |
| MS.gene050553 | MS.gene58165 | 0.811233 | 7.76E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene58165 | 0.891881 | 2.65E-74 | -1.69E-46 |
| MS.gene050612 | MS.gene58165 | 0.850479 | 1.65E-60 | -1.69E-46 |
| MS.gene050675 | MS.gene58165 | 0.847499 | 1.11E-59 | -1.69E-46 |
| MS.gene050697 | MS.gene58165 | 0.8261 | 3.26E-54 | -1.69E-46 |
| MS.gene050699 | MS.gene58165 | 0.846432 | 2.18E-59 | -1.69E-46 |
| MS.gene050747 | MS.gene58165 | 0.858499 | 7.90E-63 | -1.69E-46 |
| MS.gene050772 | MS.gene58165 | 0.828368 | 9.34E-55 | -1.69E-46 |
| MS.gene05080 | MS.gene58165 | 0.885716 | 6.40E-72 | -1.69E-46 |
| MS.gene05081 | MS.gene58165 | 0.832857 | 7.44E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene58165 | 0.901751 | 1.95E-78 | -1.69E-46 |
| MS.gene050871 | MS.gene58165 | 0.899976 | 1.16E-77 | -1.69E-46 |
| MS.gene050904 | MS.gene58165 | 0.886931 | 2.22E-72 | -1.69E-46 |
| MS.gene050950 | MS.gene58165 | 0.857209 | 1.91E-62 | -1.69E-46 |
| MS.gene050986 | MS.gene58165 | 0.833687 | 4.62E-56 | -1.69E-46 |
| MS.gene051268 | MS.gene58165 | 0.855231 | 7.25E-62 | -1.69E-46 |
| MS.gene051317 | MS.gene58165 | 0.86366 | 2.12E-64 | -1.69E-46 |
| MS.gene051438 | MS.gene58165 | 0.833627 | 4.78E-56 | -1.69E-46 |
| MS.gene051439 | MS.gene58165 | -0.819486 | 1.13E-52 | -1.69E-46 |
| MS.gene051551 | MS.gene58165 | 0.806078 | 9.80E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene58165 | -0.831738 | 1.41E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene58165 | 0.804118 | 2.52E-49 | -1.69E-46 |
| MS.gene051734 | MS.gene58165 | 0.812282 | 4.58E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene58165 | 0.893175 | 8.02E-75 | -1.69E-46 |
| MS.gene051874 | MS.gene58165 | 0.81287 | 3.41E-51 | -1.69E-46 |
| MS.gene051941 | MS.gene58165 | 0.83692 | 7.05E-57 | -1.69E-46 |
| MS.gene052066 | MS.gene58165 | 0.817064 | 4.00E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene58165 | 0.812716 | 3.68E-51 | -1.69E-46 |
| MS.gene052129 | MS.gene58165 | -0.829215 | 5.83E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene58165 | 0.880655 | 4.59E-70 | -1.69E-46 |
| MS.gene052346 | MS.gene58165 | 0.800738 | 1.25E-48 | -1.69E-46 |
| MS.gene052482 | MS.gene58165 | 0.849339 | 3.45E-60 | -1.69E-46 |
| MS.gene052486 | MS.gene58165 | 0.847794 | 9.23E-60 | -1.69E-46 |
| MS.gene052494 | MS.gene58165 | -0.821775 | 3.37E-53 | -1.69E-46 |
| MS.gene052501 | MS.gene58165 | 0.800581 | 1.35E-48 | -1.69E-46 |
| MS.gene052503 | MS.gene58165 | 0.863503 | 2.38E-64 | -1.69E-46 |
| MS.gene052506 | MS.gene58165 | 0.832486 | 9.20E-56 | -1.69E-46 |
| MS.gene052606 | MS.gene58165 | -0.819803 | 9.59E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene58165 | 0.821638 | 3.63E-53 | -1.69E-46 |
| MS.gene052822 | MS.gene58165 | 0.827977 | 1.16E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene58165 | 0.864099 | 1.55E-64 | -1.69E-46 |
| MS.gene052825 | MS.gene58165 | 0.864445 | 1.21E-64 | -1.69E-46 |
| MS.gene052870 | MS.gene58165 | 0.819835 | 9.43E-53 | -1.69E-46 |
| MS.gene052872 | MS.gene58165 | 0.837393 | 5.34E-57 | -1.69E-46 |
| MS.gene052966 | MS.gene58165 | 0.858584 | 7.45E-63 | -1.69E-46 |
| MS.gene053097 | MS.gene58165 | -0.821256 | 4.45E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene58165 | 0.888468 | 5.75E-73 | -1.69E-46 |
| MS.gene053207 | MS.gene58165 | 0.811136 | 8.14E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene58165 | 0.805182 | 1.51E-49 | -1.69E-46 |
| MS.gene053286 | MS.gene58165 | 0.805667 | 1.19E-49 | -1.69E-46 |
| MS.gene053436 | MS.gene58165 | 0.835504 | 1.62E-56 | -1.69E-46 |
| MS.gene053479 | MS.gene58165 | 0.881522 | 2.24E-70 | -1.69E-46 |
| MS.gene053480 | MS.gene58165 | 0.864714 | 9.96E-65 | -1.69E-46 |
| MS.gene053574 | MS.gene58165 | 0.839209 | 1.82E-57 | -1.69E-46 |
| MS.gene053626 | MS.gene58165 | 0.839419 | 1.60E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene58165 | 0.825859 | 3.73E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene58165 | 0.87665 | 1.18E-68 | -1.69E-46 |
| MS.gene053912 | MS.gene58165 | 0.804452 | 2.15E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene58165 | 0.823586 | 1.28E-53 | -1.69E-46 |
| MS.gene054003 | MS.gene58165 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene054107 | MS.gene58165 | 0.803874 | 2.83E-49 | -1.69E-46 |
| MS.gene054343 | MS.gene58165 | 0.808498 | 3.01E-50 | -1.69E-46 |
| MS.gene054344 | MS.gene58165 | 0.834943 | 2.24E-56 | -1.69E-46 |
| MS.gene054408 | MS.gene58165 | 0.829753 | 4.32E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene58165 | 0.841005 | 6.18E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene58165 | 0.842708 | 2.19E-58 | -1.69E-46 |
| MS.gene054633 | MS.gene58165 | -0.826206 | 3.08E-54 | -1.69E-46 |
| MS.gene054642 | MS.gene58165 | 0.806674 | 7.33E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene58165 | -0.841427 | 4.78E-58 | -1.69E-46 |
| MS.gene05470 | MS.gene58165 | 0.901887 | 1.70E-78 | -1.69E-46 |
| MS.gene054851 | MS.gene58165 | -0.81355 | 2.42E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene58165 | -0.837721 | 4.40E-57 | -1.69E-46 |
| MS.gene054927 | MS.gene58165 | 0.806671 | 7.35E-50 | -1.69E-46 |
| MS.gene054941 | MS.gene58165 | -0.80291 | 4.48E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene58165 | 0.854712 | 1.03E-61 | -1.69E-46 |
| MS.gene054954 | MS.gene58165 | 0.841753 | 3.92E-58 | -1.69E-46 |
| MS.gene055158 | MS.gene58165 | -0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene58165 | 0.801119 | 1.05E-48 | -1.69E-46 |
| MS.gene055224 | MS.gene58165 | 0.81868 | 1.73E-52 | -1.69E-46 |
| MS.gene055251 | MS.gene58165 | -0.819451 | 1.15E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene58165 | 0.834536 | 2.83E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene58165 | 0.843168 | 1.65E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene58165 | 0.88267 | 8.57E-71 | -1.69E-46 |
| MS.gene055731 | MS.gene58165 | -0.802632 | 5.12E-49 | -1.69E-46 |
| MS.gene055741 | MS.gene58165 | 0.824443 | 8.05E-54 | -1.69E-46 |
| MS.gene055744 | MS.gene58165 | 0.814503 | 1.49E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene58165 | 0.882767 | 7.90E-71 | -1.69E-46 |
| MS.gene055750 | MS.gene58165 | 0.878928 | 1.89E-69 | -1.69E-46 |
| MS.gene055751 | MS.gene58165 | 0.878136 | 3.58E-69 | -1.69E-46 |
| MS.gene055776 | MS.gene58165 | 0.822394 | 2.43E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene58165 | 0.911636 | 4.86E-83 | -1.69E-46 |
| MS.gene055941 | MS.gene58165 | 0.834691 | 2.59E-56 | -1.69E-46 |
| MS.gene056040 | MS.gene58165 | 0.820349 | 7.19E-53 | -1.69E-46 |
| MS.gene056046 | MS.gene58165 | 0.817798 | 2.74E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene58165 | 0.839947 | 1.17E-57 | -1.69E-46 |
| MS.gene056266 | MS.gene58165 | 0.85362 | 2.12E-61 | -1.69E-46 |
| MS.gene056283 | MS.gene58165 | 0.857984 | 1.12E-62 | -1.69E-46 |
| MS.gene056387 | MS.gene58165 | 0.892738 | 1.20E-74 | -1.69E-46 |
| MS.gene056446 | MS.gene58165 | 0.835848 | 1.32E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene58165 | 0.877887 | 4.38E-69 | -1.69E-46 |
| MS.gene056510 | MS.gene58165 | 0.858293 | 9.10E-63 | -1.69E-46 |
| MS.gene056583 | MS.gene58165 | -0.801328 | 9.48E-49 | -1.69E-46 |
| MS.gene056610 | MS.gene58165 | 0.803542 | 3.32E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene58165 | -0.825097 | 5.64E-54 | -1.69E-46 |
| MS.gene05664 | MS.gene58165 | 0.804486 | 2.11E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene58165 | 0.826065 | 3.33E-54 | -1.69E-46 |
| MS.gene05666 | MS.gene58165 | 0.826744 | 2.29E-54 | -1.69E-46 |
| MS.gene056692 | MS.gene58165 | 0.835443 | 1.67E-56 | -1.69E-46 |
| MS.gene056694 | MS.gene58165 | -0.828429 | 9.03E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene58165 | -0.822622 | 2.15E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene58165 | 0.831508 | 1.60E-55 | -1.69E-46 |
| MS.gene057086 | MS.gene58165 | 0.833141 | 6.33E-56 | -1.69E-46 |
| MS.gene057332 | MS.gene58165 | 0.818061 | 2.39E-52 | -1.69E-46 |
| MS.gene057367 | MS.gene58165 | -0.819827 | 9.47E-53 | -1.69E-46 |
| MS.gene05753 | MS.gene58165 | 0.800308 | 1.53E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene58165 | 0.859954 | 2.89E-63 | -1.69E-46 |
| MS.gene057734 | MS.gene58165 | 0.841847 | 3.71E-58 | -1.69E-46 |
| MS.gene057831 | MS.gene58165 | 0.865038 | 7.89E-65 | -1.69E-46 |
| MS.gene057857 | MS.gene58165 | 0.821451 | 4.01E-53 | -1.69E-46 |
| MS.gene05787 | MS.gene58165 | 0.873169 | 1.81E-67 | -1.69E-46 |
| MS.gene058130 | MS.gene58165 | 0.814768 | 1.30E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene58165 | 0.824857 | 6.43E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene58165 | -0.860801 | 1.60E-63 | -1.69E-46 |
| MS.gene058338 | MS.gene58165 | 0.833203 | 6.10E-56 | -1.69E-46 |
| MS.gene05837 | MS.gene58165 | 0.865149 | 7.28E-65 | -1.69E-46 |
| MS.gene058382 | MS.gene58165 | -0.81637 | 5.73E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene58165 | -0.812651 | 3.81E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene58165 | 0.83683 | 7.43E-57 | -1.69E-46 |
| MS.gene058448 | MS.gene58165 | 0.821791 | 3.35E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene58165 | 0.855652 | 5.46E-62 | -1.69E-46 |
| MS.gene058773 | MS.gene58165 | 0.831446 | 1.66E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene58165 | 0.875898 | 2.14E-68 | -1.69E-46 |
| MS.gene058853 | MS.gene58165 | -0.800951 | 1.13E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene58165 | 0.830776 | 2.43E-55 | -1.69E-46 |
| MS.gene059023 | MS.gene58165 | 0.888125 | 7.79E-73 | -1.69E-46 |
| MS.gene05914 | MS.gene58165 | 0.841857 | 3.68E-58 | -1.69E-46 |
| MS.gene05926 | MS.gene58165 | -0.845476 | 3.97E-59 | -1.69E-46 |
| MS.gene059303 | MS.gene58165 | 0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene58165 | 0.800338 | 1.51E-48 | -1.69E-46 |
| MS.gene059367 | MS.gene58165 | 0.804952 | 1.69E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene58165 | -0.802877 | 4.55E-49 | -1.69E-46 |
| MS.gene05960 | MS.gene58165 | -0.835099 | 2.04E-56 | -1.69E-46 |
| MS.gene059667 | MS.gene58165 | 0.87014 | 1.83E-66 | -1.69E-46 |
| MS.gene059677 | MS.gene58165 | 0.836336 | 9.93E-57 | -1.69E-46 |
| MS.gene059699 | MS.gene58165 | -0.814049 | 1.88E-51 | -1.69E-46 |
| MS.gene059703 | MS.gene58165 | -0.819694 | 1.02E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene58165 | 0.846955 | 1.57E-59 | -1.69E-46 |
| MS.gene059756 | MS.gene58165 | 0.821438 | 4.04E-53 | -1.69E-46 |
| MS.gene059837 | MS.gene58165 | 0.842846 | 2.01E-58 | -1.69E-46 |
| MS.gene059865 | MS.gene58165 | 0.867258 | 1.56E-65 | -1.69E-46 |
| MS.gene05995 | MS.gene58165 | 0.800696 | 1.28E-48 | -1.69E-46 |
| MS.gene05996 | MS.gene58165 | 0.825156 | 5.47E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene58165 | 0.90657 | 1.29E-80 | -1.69E-46 |
| MS.gene06027 | MS.gene58165 | 0.868616 | 5.72E-66 | -1.69E-46 |
| MS.gene060405 | MS.gene58165 | -0.813589 | 2.37E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene58165 | -0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene58165 | 0.811987 | 5.32E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene58165 | 0.875049 | 4.18E-68 | -1.69E-46 |
| MS.gene060778 | MS.gene58165 | 0.863608 | 2.20E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene58165 | 0.854133 | 1.51E-61 | -1.69E-46 |
| MS.gene06080 | MS.gene58165 | -0.814065 | 1.86E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene58165 | 0.816323 | 5.87E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene58165 | 0.831804 | 1.36E-55 | -1.69E-46 |
| MS.gene06097 | MS.gene58165 | 0.84187 | 3.66E-58 | -1.69E-46 |
| MS.gene060982 | MS.gene58165 | 0.897591 | 1.21E-76 | -1.69E-46 |
| MS.gene061044 | MS.gene58165 | -0.84876 | 4.99E-60 | -1.69E-46 |
| MS.gene061150 | MS.gene58165 | 0.825879 | 3.68E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58165.t1 | MTR_8g092030 | 94.944 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 3.65e-111 | 314 |
| MS.gene58165.t1 | MTR_8g092030 | 94.706 | 170 | 8 | 1 | 3 | 171 | 1 | 170 | 6.05e-105 | 298 |
| MS.gene58165.t1 | MTR_1g060070 | 36.134 | 119 | 73 | 2 | 57 | 175 | 8 | 123 | 1.53e-21 | 85.5 |
| MS.gene58165.t1 | MTR_7g104600 | 34.711 | 121 | 73 | 4 | 58 | 175 | 39 | 156 | 1.43e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58165.t1 | AT1G10500 | 64.835 | 182 | 52 | 3 | 7 | 179 | 2 | 180 | 2.21e-76 | 226 |
| MS.gene58165.t1 | AT2G16710 | 37.719 | 114 | 63 | 3 | 67 | 175 | 14 | 124 | 1.28e-21 | 85.9 |
| MS.gene58165.t1 | AT2G16710 | 35.593 | 118 | 66 | 3 | 67 | 175 | 14 | 130 | 3.82e-21 | 84.7 |
| MS.gene58165.t1 | AT2G36260 | 33.962 | 106 | 66 | 3 | 70 | 175 | 5 | 106 | 1.17e-17 | 74.7 |
| MS.gene58165.t1 | AT5G03905 | 34.234 | 111 | 70 | 2 | 70 | 179 | 50 | 158 | 3.37e-17 | 75.1 |
| MS.gene58165.t1 | AT2G16710 | 35.366 | 82 | 50 | 2 | 70 | 151 | 22 | 100 | 7.44e-13 | 62.4 |
Find 42 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGCCTACTCTTCGTATT+TGG | 0.197827 | 4.2:+10145008 | MS.gene58165:CDS |
| GATCGAATTGAGAGAGGTTT+AGG | 0.272109 | 4.2:-10143409 | None:intergenic |
| CCACAGGTTTGTGTCGCATT+AGG | 0.312594 | 4.2:-10145086 | None:intergenic |
| CAAGATTTGTGTTTAAGAAT+TGG | 0.321991 | 4.2:+10144100 | MS.gene58165:CDS |
| GATTGGAACGCGAACGAATT+GGG | 0.333118 | 4.2:-10143329 | None:intergenic |
| AATACGAAGAGTAGGCTCTT+AGG | 0.361492 | 4.2:-10145005 | None:intergenic |
| TGATTGGAACGCGAACGAAT+TGG | 0.382823 | 4.2:-10143330 | None:intergenic |
| GGTGCCACGGCCCCAGATGC+AGG | 0.406436 | 4.2:-10144016 | None:intergenic |
| ATCGAATTGAGAGAGGTTTA+GGG | 0.409502 | 4.2:-10143408 | None:intergenic |
| GAGAGAGAGCTATGAGTGAT+TGG | 0.409580 | 4.2:-10143346 | None:intergenic |
| TGCACCTGCATCTGGGGCCG+TGG | 0.436538 | 4.2:+10144012 | MS.gene58165:CDS |
| AAAACCTTTGTATTCAATGA+TGG | 0.441255 | 4.2:-10144200 | None:intergenic |
| CTAAAGCGGCGATTGAGAAT+TGG | 0.446890 | 4.2:-10143385 | None:intergenic |
| GTCAAACAAGGTGGGTGCTC+TGG | 0.472114 | 4.2:+10144124 | MS.gene58165:CDS |
| TTATCTGTAAGCGAAATTGC+AGG | 0.509358 | 4.2:-10144037 | None:intergenic |
| GGGCTTACCGGCAGCAATAA+CGG | 0.512066 | 4.2:-10143437 | None:intergenic |
| ATTGGAACGCGAACGAATTG+GGG | 0.513543 | 4.2:-10143328 | None:intergenic |
| AAATGATTTACCACATCCAC+AGG | 0.517325 | 4.2:-10145102 | None:intergenic |
| AAATCAAACATGGCCATGGC+TGG | 0.519320 | 4.2:+10143265 | None:intergenic |
| TATTCAATGATGGAGTCGTC+TGG | 0.526703 | 4.2:-10144190 | None:intergenic |
| TCACTTTCCGTTATTGCTGC+CGG | 0.540114 | 4.2:+10143430 | MS.gene58165:CDS |
| AGTGATGCTCTGATCGGAGG+AGG | 0.560784 | 4.2:+10145047 | MS.gene58165:CDS |
| TTTAGCTGCACCTGCATCTG+GGG | 0.577576 | 4.2:+10144006 | MS.gene58165:intron |
| GCGACACAAACCTGTGGATG+TGG | 0.581388 | 4.2:+10145092 | MS.gene58165:CDS |
| GACTACAGTGATGCTCTGAT+CGG | 0.584651 | 4.2:+10145041 | MS.gene58165:CDS |
| TTAAGAATTGGTGTCAAACA+AGG | 0.584994 | 4.2:+10144112 | MS.gene58165:CDS |
| AAGCGAAATTGCAGGTGCCA+CGG | 0.587731 | 4.2:-10144029 | None:intergenic |
| AGAATTGGTGTCAAACAAGG+TGG | 0.587930 | 4.2:+10144115 | MS.gene58165:CDS |
| TTGAGGACAGGGCTAATGCA+AGG | 0.598318 | 4.2:+10144167 | MS.gene58165:CDS |
| CAATTACTACTACCGCGCAC+TGG | 0.599076 | 4.2:+10143302 | MS.gene58165:CDS |
| AGAGAGGTTTAGGGCTAAAG+CGG | 0.601518 | 4.2:-10143399 | None:intergenic |
| GCATTCCAAATACGAAGAGT+AGG | 0.603532 | 4.2:-10145013 | None:intergenic |
| ACACAAATCAAACATGGCCA+TGG | 0.605803 | 4.2:+10143261 | None:intergenic |
| AGAGAAAGCAGCACCAGCCA+TGG | 0.608264 | 4.2:-10143278 | None:intergenic |
| CTCTGGTATGTCATACACAA+TGG | 0.609613 | 4.2:+10144141 | MS.gene58165:CDS |
| TACAGTGATGCTCTGATCGG+AGG | 0.616804 | 4.2:+10145044 | MS.gene58165:CDS |
| GAAAGTGATCGAATTGAGAG+AGG | 0.627615 | 4.2:-10143415 | None:intergenic |
| TCAATCACTTGAATAAGATG+AGG | 0.638619 | 4.2:+10144065 | MS.gene58165:CDS |
| GAATTGGTGTCAAACAAGGT+GGG | 0.648767 | 4.2:+10144116 | MS.gene58165:CDS |
| GACTCCATCATTGAATACAA+AGG | 0.682577 | 4.2:+10144196 | MS.gene58165:CDS |
| CCTAATGCGACACAAACCTG+TGG | 0.690717 | 4.2:+10145086 | MS.gene58165:CDS |
| GAATTGGGGAGACCAGTGCG+CGG | 0.788727 | 4.2:-10143314 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATAAATAGAAGAATAT+GGG | - | chr4.2:10143461-10143480 | None:intergenic | 10.0% |
| !! | AAAATAAAGAGAGAAAAAAA+TGG | + | chr4.2:10143861-10143880 | MS.gene58165:intron | 15.0% |
| !! | AAATAAAGAGAGAAAAAAAT+GGG | + | chr4.2:10143862-10143881 | MS.gene58165:intron | 15.0% |
| !! | GATTAATAAATAGAAGAATA+TGG | - | chr4.2:10143462-10143481 | None:intergenic | 15.0% |
| !! | TTAATAAATAGAAGAATATG+GGG | - | chr4.2:10143460-10143479 | None:intergenic | 15.0% |
| !!! | ATATTTTGCTTGTTTTTATT+TGG | + | chr4.2:10144453-10144472 | MS.gene58165:intron | 15.0% |
| !!! | TATTTTGCTTGTTTTTATTT+GGG | + | chr4.2:10144454-10144473 | MS.gene58165:intron | 15.0% |
| !! | AGGAAATAACAAAACATAAA+TGG | - | chr4.2:10143675-10143694 | None:intergenic | 20.0% |
| !! | ATCAAATAATTTAATCACCT+TGG | - | chr4.2:10144306-10144325 | None:intergenic | 20.0% |
| !! | CAAAATACTCCTATTAATTA+GGG | - | chr4.2:10143639-10143658 | None:intergenic | 20.0% |
| !! | CAATATCTTCAAATGAATAT+GGG | + | chr4.2:10144342-10144361 | MS.gene58165:intron | 20.0% |
| !! | TCAAAATACTCCTATTAATT+AGG | - | chr4.2:10143640-10143659 | None:intergenic | 20.0% |
| !!! | AATTAAGATTTTCATCGATA+CGG | - | chr4.2:10144371-10144390 | None:intergenic | 20.0% |
| !!! | AGTTTGATAACATTTATACT+TGG | - | chr4.2:10144494-10144513 | None:intergenic | 20.0% |
| !!! | CAGTTCAAACTTTTTTTTTA+TGG | + | chr4.2:10144870-10144889 | MS.gene58165:intron | 20.0% |
| !!! | TTTATTTTAAACTCATAGCA+TGG | - | chr4.2:10143849-10143868 | None:intergenic | 20.0% |
| ! | AAAACCTTTGTATTCAATGA+TGG | - | chr4.2:10144203-10144222 | None:intergenic | 25.0% |
| ! | ATTACGACAATTTCATGAAT+TGG | - | chr4.2:10143521-10143540 | None:intergenic | 25.0% |
| ! | CAAGATTTGTGTTTAAGAAT+TGG | + | chr4.2:10144100-10144119 | MS.gene58165:CDS | 25.0% |
| ! | CAATGATAATAAAAACTGAG+TGG | + | chr4.2:10144521-10144540 | MS.gene58165:intron | 25.0% |
| ! | CCAATATCTTCAAATGAATA+TGG | + | chr4.2:10144341-10144360 | MS.gene58165:intron | 25.0% |
| ! | CCATATTCATTTGAAGATAT+TGG | - | chr4.2:10144344-10144363 | None:intergenic | 25.0% |
| ! | GCAAATGTTGAATAAATACA+TGG | - | chr4.2:10143973-10143992 | None:intergenic | 25.0% |
| !! | ATTAAGATTTTCATCGATAC+GGG | - | chr4.2:10144370-10144389 | None:intergenic | 25.0% |
| !! | CTTTTAGTAGTAATTAAGCT+TGG | + | chr4.2:10144907-10144926 | MS.gene58165:intron | 25.0% |
| !!! | AGGAGTATTTTGAAAGATTA+AGG | + | chr4.2:10143647-10143666 | MS.gene58165:intron | 25.0% |
| !!! | ATTAAACTTTTTTCGCTGAT+AGG | + | chr4.2:10144555-10144574 | MS.gene58165:intron | 25.0% |
| !!! | CTTTTTAAGCTTTTGTTTAC+TGG | + | chr4.2:10144820-10144839 | MS.gene58165:intron | 25.0% |
| ACAGATATGATAGTTAACAG+AGG | - | chr4.2:10144609-10144628 | None:intergenic | 30.0% | |
| ATACTCCTATTAATTAGGGT+AGG | - | chr4.2:10143635-10143654 | None:intergenic | 30.0% | |
| TCAATCACTTGAATAAGATG+AGG | + | chr4.2:10144065-10144084 | MS.gene58165:CDS | 30.0% | |
| TTAAGACTTGATTGAAAGCT+TGG | + | chr4.2:10144771-10144790 | MS.gene58165:intron | 30.0% | |
| !! | GAATACAAAGGTTTTGTCAT+TGG | + | chr4.2:10144208-10144227 | MS.gene58165:CDS | 30.0% |
| !! | TTAAGAATTGGTGTCAAACA+AGG | + | chr4.2:10144112-10144131 | MS.gene58165:CDS | 30.0% |
| !! | TTGTTATTTCCTATGAACCA+CGG | + | chr4.2:10143683-10143702 | MS.gene58165:intron | 30.0% |
| !!! | TTTTTTTTATGGCTGCACTT+GGG | + | chr4.2:10144881-10144900 | MS.gene58165:intron | 30.0% |
| !!! | TTTTTTTTTATGGCTGCACT+TGG | + | chr4.2:10144880-10144899 | MS.gene58165:intron | 30.0% |
| AAATGATTTACCACATCCAC+AGG | - | chr4.2:10145105-10145124 | None:intergenic | 35.0% | |
| AAGAACTGAAGAAGATAACG+TGG | - | chr4.2:10144803-10144822 | None:intergenic | 35.0% | |
| ATCGAATTGAGAGAGGTTTA+GGG | - | chr4.2:10143411-10143430 | None:intergenic | 35.0% | |
| ATTAATTAGGGTAGGCAAAG+CGG | - | chr4.2:10143627-10143646 | None:intergenic | 35.0% | |
| CTTTGCCTACCCTAATTAAT+AGG | + | chr4.2:10143627-10143646 | MS.gene58165:intron | 35.0% | |
| GACTCCATCATTGAATACAA+AGG | + | chr4.2:10144196-10144215 | MS.gene58165:CDS | 35.0% | |
| GGGGTTATGCAGAAAAATTA+AGG | - | chr4.2:10143606-10143625 | None:intergenic | 35.0% | |
| TTATCTGTAAGCGAAATTGC+AGG | - | chr4.2:10144040-10144059 | None:intergenic | 35.0% | |
| ! | CTTTACACTCAGATTAGTCA+GGG | + | chr4.2:10143803-10143822 | MS.gene58165:intron | 35.0% |
| ! | TCTTTACACTCAGATTAGTC+AGG | + | chr4.2:10143802-10143821 | MS.gene58165:intron | 35.0% |
| ! | TGTTGTTGTTGTTGTACAGT+GGG | + | chr4.2:10144406-10144425 | MS.gene58165:intron | 35.0% |
| ! | TTGTTGTTGTTGTTGTACAG+TGG | + | chr4.2:10144405-10144424 | MS.gene58165:intron | 35.0% |
| ! | TTTTCCTGCTCTTTCTCTTT+TGG | + | chr4.2:10144279-10144298 | MS.gene58165:intron | 35.0% |
| !! | GTCATACACAATGGATTTTG+AGG | + | chr4.2:10144150-10144169 | MS.gene58165:CDS | 35.0% |
| !!! | TTACTGGTTTTTGCAGCAAT+TGG | + | chr4.2:10144836-10144855 | MS.gene58165:intron | 35.0% |
| AACTGAAGAAGATAACGTGG+AGG | - | chr4.2:10144800-10144819 | None:intergenic | 40.0% | |
| AAGAGCCTACTCTTCGTATT+TGG | + | chr4.2:10145008-10145027 | MS.gene58165:CDS | 40.0% | |
| AATACGAAGAGTAGGCTCTT+AGG | - | chr4.2:10145008-10145027 | None:intergenic | 40.0% | |
| ATTCCTGCTTAACAAAGTGC+TGG | - | chr4.2:10144734-10144753 | None:intergenic | 40.0% | |
| CTCTGGTATGTCATACACAA+TGG | + | chr4.2:10144141-10144160 | MS.gene58165:CDS | 40.0% | |
| GAAAGTGATCGAATTGAGAG+AGG | - | chr4.2:10143418-10143437 | None:intergenic | 40.0% | |
| GAGATAAGAAATCACCCATG+TGG | - | chr4.2:10143721-10143740 | None:intergenic | 40.0% | |
| GATCGAATTGAGAGAGGTTT+AGG | - | chr4.2:10143412-10143431 | None:intergenic | 40.0% | |
| GCATTCCAAATACGAAGAGT+AGG | - | chr4.2:10145016-10145035 | None:intergenic | 40.0% | |
| TAAGCTTGGCAAACGAAAGA+AGG | + | chr4.2:10144921-10144940 | MS.gene58165:intron | 40.0% | |
| TAGAAGAATATGGGGCTTAC+CGG | - | chr4.2:10143452-10143471 | None:intergenic | 40.0% | |
| TTAATTAGGGTAGGCAAAGC+GGG | - | chr4.2:10143626-10143645 | None:intergenic | 40.0% | |
| ! | TATTCAATGATGGAGTCGTC+TGG | - | chr4.2:10144193-10144212 | None:intergenic | 40.0% |
| !! | ACACAATGGATTTTGAGGAC+AGG | + | chr4.2:10144155-10144174 | MS.gene58165:CDS | 40.0% |
| !! | AGAATTGGTGTCAAACAAGG+TGG | + | chr4.2:10144115-10144134 | MS.gene58165:CDS | 40.0% |
| !! | CACAATGGATTTTGAGGACA+GGG | + | chr4.2:10144156-10144175 | MS.gene58165:CDS | 40.0% |
| !! | GAATTGGTGTCAAACAAGGT+GGG | + | chr4.2:10144116-10144135 | MS.gene58165:CDS | 40.0% |
| !!! | GTGGGTGTTTTATTTCAACG+CGG | + | chr4.2:10144424-10144443 | MS.gene58165:intron | 40.0% |
| !!! | TGGGTGTTTTATTTCAACGC+GGG | + | chr4.2:10144425-10144444 | MS.gene58165:intron | 40.0% |
| AAGAAATCACCCATGTGGCA+TGG | - | chr4.2:10143716-10143735 | None:intergenic | 45.0% | |
| ATTGGAACGCGAACGAATTG+GGG | - | chr4.2:10143331-10143350 | None:intergenic | 45.0% | |
| CAAACCTAGCAGCAAGTACT+GGG | - | chr4.2:10143557-10143576 | None:intergenic | 45.0% | |
| CTAAAGCGGCGATTGAGAAT+TGG | - | chr4.2:10143388-10143407 | None:intergenic | 45.0% | |
| GACTACAGTGATGCTCTGAT+CGG | + | chr4.2:10145041-10145060 | MS.gene58165:CDS | 45.0% | |
| GAGAGAGAGCTATGAGTGAT+TGG | - | chr4.2:10143349-10143368 | None:intergenic | 45.0% | |
| GATTGGAACGCGAACGAATT+GGG | - | chr4.2:10143332-10143351 | None:intergenic | 45.0% | |
| TAATTAGGGTAGGCAAAGCG+GGG | - | chr4.2:10143625-10143644 | None:intergenic | 45.0% | |
| TCACTTTCCGTTATTGCTGC+CGG | + | chr4.2:10143430-10143449 | MS.gene58165:CDS | 45.0% | |
| TGATTGGAACGCGAACGAAT+TGG | - | chr4.2:10143333-10143352 | None:intergenic | 45.0% | |
| TGGACCAAAAGAGAAAGAGC+AGG | - | chr4.2:10144286-10144305 | None:intergenic | 45.0% | |
| ! | AAACCTAGCAGCAAGTACTG+GGG | - | chr4.2:10143556-10143575 | None:intergenic | 45.0% |
| ! | AGAGAGGTTTAGGGCTAAAG+CGG | - | chr4.2:10143402-10143421 | None:intergenic | 45.0% |
| ! | GCTCTTTCTCTTTTGGTCCA+AGG | + | chr4.2:10144286-10144305 | MS.gene58165:intron | 45.0% |
| ! | TCAGGGTTCGTTGTGACTTT+TGG | + | chr4.2:10143820-10143839 | MS.gene58165:intron | 45.0% |
| !! | CTGGTTTTTGCAGCAATTGG+TGG | + | chr4.2:10144839-10144858 | MS.gene58165:intron | 45.0% |
| !! | TTTTAGCTGCACCTGCATCT+GGG | + | chr4.2:10144005-10144024 | MS.gene58165:intron | 45.0% |
| !!! | ATTTTAGCTGCACCTGCATC+TGG | + | chr4.2:10144004-10144023 | MS.gene58165:intron | 45.0% |
| AAGCGAAATTGCAGGTGCCA+CGG | - | chr4.2:10144032-10144051 | None:intergenic | 50.0% | |
| CAATTACTACTACCGCGCAC+TGG | + | chr4.2:10143302-10143321 | MS.gene58165:CDS | 50.0% | |
| CCAAACCTAGCAGCAAGTAC+TGG | - | chr4.2:10143558-10143577 | None:intergenic | 50.0% | |
| CCACAGGTTTGTGTCGCATT+AGG | - | chr4.2:10145089-10145108 | None:intergenic | 50.0% | |
| CCTAATGCGACACAAACCTG+TGG | + | chr4.2:10145086-10145105 | MS.gene58165:CDS | 50.0% | |
| CTGCCAGCACTTTGTTAAGC+AGG | + | chr4.2:10144728-10144747 | MS.gene58165:intron | 50.0% | |
| TACAGTGATGCTCTGATCGG+AGG | + | chr4.2:10145044-10145063 | MS.gene58165:CDS | 50.0% | |
| TACATGGTCGAGACAGACAC+AGG | - | chr4.2:10143957-10143976 | None:intergenic | 50.0% | |
| TTGAGGACAGGGCTAATGCA+AGG | + | chr4.2:10144167-10144186 | MS.gene58165:CDS | 50.0% | |
| TTTAGCTGCACCTGCATCTG+GGG | + | chr4.2:10144006-10144025 | MS.gene58165:intron | 50.0% | |
| ! | CCAGTACTTGCTGCTAGGTT+TGG | + | chr4.2:10143555-10143574 | MS.gene58165:intron | 50.0% |
| ! | GGGAGTGCAACAAAGCACTT+CGG | + | chr4.2:10143882-10143901 | MS.gene58165:intron | 50.0% |
| AGAGAAAGCAGCACCAGCCA+TGG | - | chr4.2:10143281-10143300 | None:intergenic | 55.0% | |
| AGTGATGCTCTGATCGGAGG+AGG | + | chr4.2:10145047-10145066 | MS.gene58165:CDS | 55.0% | |
| GCGACACAAACCTGTGGATG+TGG | + | chr4.2:10145092-10145111 | MS.gene58165:CDS | 55.0% | |
| GGACACATGCCATGCCACAT+GGG | + | chr4.2:10143704-10143723 | MS.gene58165:intron | 55.0% | |
| GGCATGTGTCCGTGGTTCAT+AGG | - | chr4.2:10143695-10143714 | None:intergenic | 55.0% | |
| GGGCTTACCGGCAGCAATAA+CGG | - | chr4.2:10143440-10143459 | None:intergenic | 55.0% | |
| GTCAAACAAGGTGGGTGCTC+TGG | + | chr4.2:10144124-10144143 | MS.gene58165:CDS | 55.0% | |
| ! | AGACCCCAGTACTTGCTGCT+AGG | + | chr4.2:10143550-10143569 | MS.gene58165:intron | 55.0% |
| CGGACACATGCCATGCCACA+TGG | + | chr4.2:10143703-10143722 | MS.gene58165:intron | 60.0% | |
| GAATTGGGGAGACCAGTGCG+CGG | - | chr4.2:10143317-10143336 | None:intergenic | 60.0% | |
| TGTGGCATGGCATGTGTCCG+TGG | - | chr4.2:10143703-10143722 | None:intergenic | 60.0% | |
| ! | TGCACCTGCATCTGGGGCCG+TGG | + | chr4.2:10144012-10144031 | MS.gene58165:CDS | 70.0% |
| ! | GGTGCCACGGCCCCAGATGC+AGG | - | chr4.2:10144019-10144038 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 10143274 | 10145139 | 10143274 | ID=MS.gene58165 |
| chr4.2 | mRNA | 10143274 | 10145139 | 10143274 | ID=MS.gene58165.t1;Parent=MS.gene58165 |
| chr4.2 | exon | 10143274 | 10143451 | 10143274 | ID=MS.gene58165.t1.exon1;Parent=MS.gene58165.t1 |
| chr4.2 | CDS | 10143274 | 10143451 | 10143274 | ID=cds.MS.gene58165.t1;Parent=MS.gene58165.t1 |
| chr4.2 | exon | 10144011 | 10144229 | 10144011 | ID=MS.gene58165.t1.exon2;Parent=MS.gene58165.t1 |
| chr4.2 | CDS | 10144011 | 10144229 | 10144011 | ID=cds.MS.gene58165.t1;Parent=MS.gene58165.t1 |
| chr4.2 | exon | 10144997 | 10145139 | 10144997 | ID=MS.gene58165.t1.exon3;Parent=MS.gene58165.t1 |
| chr4.2 | CDS | 10144997 | 10145139 | 10144997 | ID=cds.MS.gene58165.t1;Parent=MS.gene58165.t1 |
| Gene Sequence |
| Protein sequence |
