Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70306.t1 | XP_003630120.1 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.90E-87 | 331.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70306.t1 | Q9XIK3 | 65.4 | 185 | 46 | 4 | 7 | 179 | 2 | 180 | 7.7e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70306.t1 | G7LBB6 | 94.9 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 2.1e-87 | 331.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene70306 | 0.872975 | 2.10E-67 | -1.69E-46 |
| MS.gene049222 | MS.gene70306 | 0.86886 | 4.77E-66 | -1.69E-46 |
| MS.gene049232 | MS.gene70306 | 0.81069 | 1.02E-50 | -1.69E-46 |
| MS.gene049251 | MS.gene70306 | -0.81132 | 7.43E-51 | -1.69E-46 |
| MS.gene049257 | MS.gene70306 | 0.812181 | 4.82E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene70306 | 0.824422 | 8.14E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene70306 | 0.822181 | 2.72E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene70306 | 0.832743 | 7.94E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene70306 | 0.875975 | 2.02E-68 | -1.69E-46 |
| MS.gene049757 | MS.gene70306 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene70306 | 0.906315 | 1.69E-80 | -1.69E-46 |
| MS.gene050315 | MS.gene70306 | 0.807021 | 6.20E-50 | -1.69E-46 |
| MS.gene050323 | MS.gene70306 | 0.866619 | 2.50E-65 | -1.69E-46 |
| MS.gene050338 | MS.gene70306 | -0.840638 | 7.71E-58 | -1.69E-46 |
| MS.gene050444 | MS.gene70306 | 0.817918 | 2.57E-52 | -1.69E-46 |
| MS.gene050496 | MS.gene70306 | 0.871434 | 6.86E-67 | -1.69E-46 |
| MS.gene050504 | MS.gene70306 | 0.854696 | 1.04E-61 | -1.69E-46 |
| MS.gene050518 | MS.gene70306 | 0.812687 | 3.74E-51 | -1.69E-46 |
| MS.gene050538 | MS.gene70306 | 0.81767 | 2.92E-52 | -1.69E-46 |
| MS.gene050553 | MS.gene70306 | 0.83907 | 1.97E-57 | -1.69E-46 |
| MS.gene050577 | MS.gene70306 | 0.891599 | 3.43E-74 | -1.69E-46 |
| MS.gene050612 | MS.gene70306 | 0.845394 | 4.17E-59 | -1.69E-46 |
| MS.gene050675 | MS.gene70306 | 0.835288 | 1.83E-56 | -1.69E-46 |
| MS.gene050697 | MS.gene70306 | 0.806112 | 9.64E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene70306 | 0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene050747 | MS.gene70306 | 0.873127 | 1.87E-67 | -1.69E-46 |
| MS.gene050772 | MS.gene70306 | 0.81516 | 1.07E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene70306 | 0.890547 | 8.93E-74 | -1.69E-46 |
| MS.gene05081 | MS.gene70306 | 0.832649 | 8.38E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene70306 | 0.896978 | 2.19E-76 | -1.69E-46 |
| MS.gene050871 | MS.gene70306 | 0.894323 | 2.75E-75 | -1.69E-46 |
| MS.gene050904 | MS.gene70306 | 0.884008 | 2.77E-71 | -1.69E-46 |
| MS.gene050950 | MS.gene70306 | 0.871321 | 7.47E-67 | -1.69E-46 |
| MS.gene050986 | MS.gene70306 | 0.830728 | 2.49E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene70306 | -0.80855 | 2.93E-50 | -1.69E-46 |
| MS.gene051268 | MS.gene70306 | 0.847542 | 1.08E-59 | -1.69E-46 |
| MS.gene051317 | MS.gene70306 | 0.862402 | 5.20E-64 | -1.69E-46 |
| MS.gene051438 | MS.gene70306 | 0.824231 | 9.03E-54 | -1.69E-46 |
| MS.gene051439 | MS.gene70306 | -0.813989 | 1.94E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene70306 | 0.800081 | 1.70E-48 | -1.69E-46 |
| MS.gene051651 | MS.gene70306 | -0.825558 | 4.39E-54 | -1.69E-46 |
| MS.gene051666 | MS.gene70306 | 0.803398 | 3.55E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene70306 | 0.900401 | 7.60E-78 | -1.69E-46 |
| MS.gene051874 | MS.gene70306 | 0.806696 | 7.26E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene70306 | 0.821926 | 3.11E-53 | -1.69E-46 |
| MS.gene052100 | MS.gene70306 | 0.820537 | 6.51E-53 | -1.69E-46 |
| MS.gene052129 | MS.gene70306 | -0.827047 | 1.94E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene70306 | 0.891359 | 4.27E-74 | -1.69E-46 |
| MS.gene052482 | MS.gene70306 | 0.841455 | 4.70E-58 | -1.69E-46 |
| MS.gene052486 | MS.gene70306 | 0.842554 | 2.41E-58 | -1.69E-46 |
| MS.gene052494 | MS.gene70306 | -0.83988 | 1.22E-57 | -1.69E-46 |
| MS.gene052501 | MS.gene70306 | 0.806874 | 6.65E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene70306 | 0.862733 | 4.11E-64 | -1.69E-46 |
| MS.gene052506 | MS.gene70306 | 0.82154 | 3.82E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene70306 | -0.835573 | 1.55E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene70306 | 0.822966 | 1.79E-53 | -1.69E-46 |
| MS.gene052822 | MS.gene70306 | 0.813938 | 1.99E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene70306 | 0.861726 | 8.38E-64 | -1.69E-46 |
| MS.gene052825 | MS.gene70306 | 0.8646 | 1.08E-64 | -1.69E-46 |
| MS.gene052870 | MS.gene70306 | 0.804606 | 1.99E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene70306 | 0.824451 | 8.02E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene70306 | 0.859173 | 4.97E-63 | -1.69E-46 |
| MS.gene053097 | MS.gene70306 | -0.849561 | 2.99E-60 | -1.69E-46 |
| MS.gene053108 | MS.gene70306 | -0.808117 | 3.63E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene70306 | 0.89539 | 1.00E-75 | -1.69E-46 |
| MS.gene053207 | MS.gene70306 | 0.807822 | 4.19E-50 | -1.69E-46 |
| MS.gene053436 | MS.gene70306 | 0.839092 | 1.95E-57 | -1.69E-46 |
| MS.gene053479 | MS.gene70306 | 0.86774 | 1.09E-65 | -1.69E-46 |
| MS.gene053480 | MS.gene70306 | 0.846299 | 2.37E-59 | -1.69E-46 |
| MS.gene053574 | MS.gene70306 | 0.840322 | 9.33E-58 | -1.69E-46 |
| MS.gene053579 | MS.gene70306 | 0.803254 | 3.81E-49 | -1.69E-46 |
| MS.gene053626 | MS.gene70306 | 0.838619 | 2.58E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene70306 | 0.822211 | 2.67E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene70306 | 0.872832 | 2.35E-67 | -1.69E-46 |
| MS.gene053916 | MS.gene70306 | 0.804794 | 1.82E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene70306 | 0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene054343 | MS.gene70306 | 0.820136 | 8.05E-53 | -1.69E-46 |
| MS.gene054344 | MS.gene70306 | 0.838009 | 3.71E-57 | -1.69E-46 |
| MS.gene054408 | MS.gene70306 | 0.84184 | 3.72E-58 | -1.69E-46 |
| MS.gene054595 | MS.gene70306 | 0.834389 | 3.08E-56 | -1.69E-46 |
| MS.gene054600 | MS.gene70306 | 0.834176 | 3.49E-56 | -1.69E-46 |
| MS.gene054633 | MS.gene70306 | -0.820458 | 6.79E-53 | -1.69E-46 |
| MS.gene054662 | MS.gene70306 | -0.804416 | 2.18E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene70306 | -0.841839 | 3.72E-58 | -1.69E-46 |
| MS.gene05470 | MS.gene70306 | 0.910281 | 2.23E-82 | -1.69E-46 |
| MS.gene054851 | MS.gene70306 | -0.815182 | 1.05E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene70306 | -0.847203 | 1.34E-59 | -1.69E-46 |
| MS.gene054927 | MS.gene70306 | 0.817954 | 2.52E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene70306 | 0.844696 | 6.44E-59 | -1.69E-46 |
| MS.gene054954 | MS.gene70306 | 0.834927 | 2.26E-56 | -1.69E-46 |
| MS.gene055158 | MS.gene70306 | -0.826127 | 3.22E-54 | -1.69E-46 |
| MS.gene055159 | MS.gene70306 | -0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene70306 | 0.815959 | 7.07E-52 | -1.69E-46 |
| MS.gene055251 | MS.gene70306 | -0.816402 | 5.63E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene70306 | 0.830117 | 3.52E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene70306 | 0.845334 | 4.33E-59 | -1.69E-46 |
| MS.gene055539 | MS.gene70306 | 0.804993 | 1.65E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene70306 | 0.890789 | 7.17E-74 | -1.69E-46 |
| MS.gene055616 | MS.gene70306 | -0.828117 | 1.07E-54 | -1.69E-46 |
| MS.gene055729 | MS.gene70306 | -0.815849 | 7.49E-52 | -1.69E-46 |
| MS.gene055730 | MS.gene70306 | -0.816346 | 5.80E-52 | -1.69E-46 |
| MS.gene055731 | MS.gene70306 | -0.823307 | 1.49E-53 | -1.69E-46 |
| MS.gene055741 | MS.gene70306 | 0.82637 | 2.82E-54 | -1.69E-46 |
| MS.gene055744 | MS.gene70306 | 0.817786 | 2.75E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene70306 | 0.89834 | 5.84E-77 | -1.69E-46 |
| MS.gene055750 | MS.gene70306 | 0.88078 | 4.14E-70 | -1.69E-46 |
| MS.gene055751 | MS.gene70306 | 0.888824 | 4.19E-73 | -1.69E-46 |
| MS.gene055776 | MS.gene70306 | 0.811434 | 7.02E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene70306 | 0.915735 | 4.13E-85 | -1.69E-46 |
| MS.gene055941 | MS.gene70306 | 0.819694 | 1.02E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene70306 | 0.833179 | 6.19E-56 | -1.69E-46 |
| MS.gene056166 | MS.gene70306 | 0.840672 | 7.55E-58 | -1.69E-46 |
| MS.gene056266 | MS.gene70306 | 0.864659 | 1.04E-64 | -1.69E-46 |
| MS.gene056283 | MS.gene70306 | 0.863601 | 2.22E-64 | -1.69E-46 |
| MS.gene056387 | MS.gene70306 | 0.87996 | 8.13E-70 | -1.69E-46 |
| MS.gene056446 | MS.gene70306 | 0.830597 | 2.69E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene70306 | 0.878437 | 2.81E-69 | -1.69E-46 |
| MS.gene056510 | MS.gene70306 | 0.853052 | 3.08E-61 | -1.69E-46 |
| MS.gene056610 | MS.gene70306 | 0.810333 | 1.21E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene70306 | -0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene70306 | 0.813802 | 2.13E-51 | -1.69E-46 |
| MS.gene05666 | MS.gene70306 | 0.813836 | 2.09E-51 | -1.69E-46 |
| MS.gene056678 | MS.gene70306 | -0.803382 | 3.58E-49 | -1.69E-46 |
| MS.gene056692 | MS.gene70306 | 0.840611 | 7.84E-58 | -1.69E-46 |
| MS.gene056694 | MS.gene70306 | -0.824676 | 7.09E-54 | -1.69E-46 |
| MS.gene056697 | MS.gene70306 | -0.810266 | 1.26E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene70306 | -0.80014 | 1.66E-48 | -1.69E-46 |
| MS.gene056877 | MS.gene70306 | 0.804289 | 2.32E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene70306 | 0.809343 | 1.98E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene70306 | 0.825389 | 4.81E-54 | -1.69E-46 |
| MS.gene057332 | MS.gene70306 | 0.818474 | 1.92E-52 | -1.69E-46 |
| MS.gene057367 | MS.gene70306 | -0.81352 | 2.46E-51 | -1.69E-46 |
| MS.gene057658 | MS.gene70306 | 0.871515 | 6.44E-67 | -1.69E-46 |
| MS.gene057734 | MS.gene70306 | 0.815816 | 7.62E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene70306 | 0.862246 | 5.81E-64 | -1.69E-46 |
| MS.gene057857 | MS.gene70306 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene70306 | 0.849736 | 2.67E-60 | -1.69E-46 |
| MS.gene058130 | MS.gene70306 | 0.817349 | 3.45E-52 | -1.69E-46 |
| MS.gene058157 | MS.gene70306 | 0.830643 | 2.62E-55 | -1.69E-46 |
| MS.gene058334 | MS.gene70306 | -0.865404 | 6.05E-65 | -1.69E-46 |
| MS.gene058338 | MS.gene70306 | 0.816768 | 4.66E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene70306 | 0.872874 | 2.27E-67 | -1.69E-46 |
| MS.gene058418 | MS.gene70306 | -0.834909 | 2.28E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene70306 | 0.826203 | 3.09E-54 | -1.69E-46 |
| MS.gene058448 | MS.gene70306 | 0.832925 | 7.15E-56 | -1.69E-46 |
| MS.gene058544 | MS.gene70306 | 0.86384 | 1.87E-64 | -1.69E-46 |
| MS.gene058723 | MS.gene70306 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene70306 | 0.835249 | 1.87E-56 | -1.69E-46 |
| MS.gene058838 | MS.gene70306 | 0.885405 | 8.37E-72 | -1.69E-46 |
| MS.gene059022 | MS.gene70306 | 0.834881 | 2.32E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene70306 | 0.891666 | 3.22E-74 | -1.69E-46 |
| MS.gene059103 | MS.gene70306 | -0.807803 | 4.23E-50 | -1.69E-46 |
| MS.gene05914 | MS.gene70306 | 0.867859 | 1.00E-65 | -1.69E-46 |
| MS.gene05926 | MS.gene70306 | -0.835395 | 1.72E-56 | -1.69E-46 |
| MS.gene059303 | MS.gene70306 | 0.832355 | 9.91E-56 | -1.69E-46 |
| MS.gene059480 | MS.gene70306 | -0.809662 | 1.69E-50 | -1.69E-46 |
| MS.gene059526 | MS.gene70306 | -0.819456 | 1.15E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene70306 | -0.869607 | 2.73E-66 | -1.69E-46 |
| MS.gene059667 | MS.gene70306 | 0.881702 | 1.93E-70 | -1.69E-46 |
| MS.gene059677 | MS.gene70306 | 0.833204 | 6.10E-56 | -1.69E-46 |
| MS.gene059703 | MS.gene70306 | -0.804787 | 1.83E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene70306 | 0.852333 | 4.94E-61 | -1.69E-46 |
| MS.gene059756 | MS.gene70306 | 0.817893 | 2.60E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene70306 | 0.80161 | 8.30E-49 | -1.69E-46 |
| MS.gene059837 | MS.gene70306 | 0.837041 | 6.57E-57 | -1.69E-46 |
| MS.gene059865 | MS.gene70306 | 0.867393 | 1.41E-65 | -1.69E-46 |
| MS.gene05996 | MS.gene70306 | 0.82523 | 5.25E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene70306 | 0.90126 | 3.21E-78 | -1.69E-46 |
| MS.gene06027 | MS.gene70306 | 0.876988 | 9.01E-69 | -1.69E-46 |
| MS.gene060405 | MS.gene70306 | -0.803091 | 4.11E-49 | -1.69E-46 |
| MS.gene060593 | MS.gene70306 | -0.804146 | 2.49E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene70306 | 0.877344 | 6.78E-69 | -1.69E-46 |
| MS.gene060778 | MS.gene70306 | 0.857173 | 1.96E-62 | -1.69E-46 |
| MS.gene06077 | MS.gene70306 | 0.862215 | 5.93E-64 | -1.69E-46 |
| MS.gene06080 | MS.gene70306 | -0.826511 | 2.61E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene70306 | 0.806269 | 8.93E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene70306 | 0.840427 | 8.75E-58 | -1.69E-46 |
| MS.gene06097 | MS.gene70306 | 0.82168 | 3.55E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene70306 | 0.891581 | 3.49E-74 | -1.69E-46 |
| MS.gene061038 | MS.gene70306 | -0.80896 | 2.40E-50 | -1.69E-46 |
| MS.gene061044 | MS.gene70306 | -0.854644 | 1.07E-61 | -1.69E-46 |
| MS.gene061150 | MS.gene70306 | 0.834264 | 3.31E-56 | -1.69E-46 |
| MS.gene061231 | MS.gene70306 | 0.800856 | 1.18E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70306.t1 | MTR_8g092030 | 94.944 | 178 | 8 | 1 | 3 | 179 | 1 | 178 | 3.65e-111 | 314 |
| MS.gene70306.t1 | MTR_8g092030 | 94.706 | 170 | 8 | 1 | 3 | 171 | 1 | 170 | 6.05e-105 | 298 |
| MS.gene70306.t1 | MTR_1g060070 | 36.134 | 119 | 73 | 2 | 57 | 175 | 8 | 123 | 1.53e-21 | 85.5 |
| MS.gene70306.t1 | MTR_7g104600 | 34.711 | 121 | 73 | 4 | 58 | 175 | 39 | 156 | 1.43e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70306.t1 | AT1G10500 | 64.835 | 182 | 52 | 3 | 7 | 179 | 2 | 180 | 2.21e-76 | 226 |
| MS.gene70306.t1 | AT2G16710 | 37.719 | 114 | 63 | 3 | 67 | 175 | 14 | 124 | 1.28e-21 | 85.9 |
| MS.gene70306.t1 | AT2G16710 | 35.593 | 118 | 66 | 3 | 67 | 175 | 14 | 130 | 3.82e-21 | 84.7 |
| MS.gene70306.t1 | AT2G36260 | 33.962 | 106 | 66 | 3 | 70 | 175 | 5 | 106 | 1.17e-17 | 74.7 |
| MS.gene70306.t1 | AT5G03905 | 34.234 | 111 | 70 | 2 | 70 | 179 | 50 | 158 | 3.37e-17 | 75.1 |
| MS.gene70306.t1 | AT2G16710 | 35.366 | 82 | 50 | 2 | 70 | 151 | 22 | 100 | 7.44e-13 | 62.4 |
Find 42 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGCCTACTCTTCGTATT+TGG | 0.197827 | 4.1:+9388980 | MS.gene70306:CDS |
| GATCGAATTGAGAGAGGTTT+AGG | 0.272109 | 4.1:-9387459 | None:intergenic |
| CCACAGGTTTGTGTCGCATT+AGG | 0.312594 | 4.1:-9389058 | None:intergenic |
| CAAGATTTGTGTTTAAGAAT+TGG | 0.321991 | 4.1:+9388132 | MS.gene70306:CDS |
| GATTGGAACGCGAACGAATT+GGG | 0.333118 | 4.1:-9387379 | None:intergenic |
| AATACGAAGAGTAGGCTCTT+AGG | 0.361492 | 4.1:-9388977 | None:intergenic |
| TGATTGGAACGCGAACGAAT+TGG | 0.382823 | 4.1:-9387380 | None:intergenic |
| GGTGCCACGGCCCCAGATGC+AGG | 0.406436 | 4.1:-9388048 | None:intergenic |
| ATCGAATTGAGAGAGGTTTA+GGG | 0.409502 | 4.1:-9387458 | None:intergenic |
| GAGAGAGAGCTATGAGTGAT+TGG | 0.409580 | 4.1:-9387396 | None:intergenic |
| TGCACCTGCATCTGGGGCCG+TGG | 0.436538 | 4.1:+9388044 | MS.gene70306:CDS |
| AAAACCTTTGTATTCAATGA+TGG | 0.441255 | 4.1:-9388232 | None:intergenic |
| CTAAAGCGGCGATTGAGAAT+TGG | 0.446890 | 4.1:-9387435 | None:intergenic |
| GACTACAGTGATGCTCTGAT+TGG | 0.458599 | 4.1:+9389013 | MS.gene70306:CDS |
| GTCAAACAAGGTGGGTGCTC+TGG | 0.472114 | 4.1:+9388156 | MS.gene70306:CDS |
| TTATCTGTAAGCGAAATTGC+AGG | 0.509358 | 4.1:-9388069 | None:intergenic |
| GGGCTTACCGGCAGCAATAA+CGG | 0.512066 | 4.1:-9387487 | None:intergenic |
| ATTGGAACGCGAACGAATTG+GGG | 0.513543 | 4.1:-9387378 | None:intergenic |
| AAATGATTTACCACATCCAC+AGG | 0.517325 | 4.1:-9389074 | None:intergenic |
| AAATCAAACATGGCCATGGC+TGG | 0.519320 | 4.1:+9387315 | None:intergenic |
| TATTCAATGATGGAGTCGTC+TGG | 0.526703 | 4.1:-9388222 | None:intergenic |
| TACAGTGATGCTCTGATTGG+AGG | 0.527694 | 4.1:+9389016 | MS.gene70306:CDS |
| TCACTTTCCGTTATTGCTGC+CGG | 0.540114 | 4.1:+9387480 | MS.gene70306:CDS |
| TTTAGCTGCACCTGCATCTG+GGG | 0.577576 | 4.1:+9388038 | MS.gene70306:intron |
| GCGACACAAACCTGTGGATG+TGG | 0.581388 | 4.1:+9389064 | MS.gene70306:CDS |
| TTAAGAATTGGTGTCAAACA+AGG | 0.584994 | 4.1:+9388144 | MS.gene70306:CDS |
| AAGCGAAATTGCAGGTGCCA+CGG | 0.587731 | 4.1:-9388061 | None:intergenic |
| AGAATTGGTGTCAAACAAGG+TGG | 0.587930 | 4.1:+9388147 | MS.gene70306:CDS |
| AGTGATGCTCTGATTGGAGG+AGG | 0.589985 | 4.1:+9389019 | MS.gene70306:CDS |
| TTGAGGACAGGGCTAATGCA+AGG | 0.598318 | 4.1:+9388199 | MS.gene70306:CDS |
| CAATTACTACTACCGCGCAC+TGG | 0.599076 | 4.1:+9387352 | MS.gene70306:CDS |
| AGAGAGGTTTAGGGCTAAAG+CGG | 0.601518 | 4.1:-9387449 | None:intergenic |
| GCATTCCAAATACGAAGAGT+AGG | 0.603532 | 4.1:-9388985 | None:intergenic |
| ACACAAATCAAACATGGCCA+TGG | 0.605803 | 4.1:+9387311 | None:intergenic |
| AGAGAAAGCAGCACCAGCCA+TGG | 0.608264 | 4.1:-9387328 | None:intergenic |
| CTCTGGTATGTCATACACAA+TGG | 0.609613 | 4.1:+9388173 | MS.gene70306:CDS |
| GAAAGTGATCGAATTGAGAG+AGG | 0.627615 | 4.1:-9387465 | None:intergenic |
| TCAATCACTTGAATAAGATG+AGG | 0.638619 | 4.1:+9388097 | MS.gene70306:CDS |
| GAATTGGTGTCAAACAAGGT+GGG | 0.648767 | 4.1:+9388148 | MS.gene70306:CDS |
| GACTCCATCATTGAATACAA+AGG | 0.682577 | 4.1:+9388228 | MS.gene70306:CDS |
| CCTAATGCGACACAAACCTG+TGG | 0.690717 | 4.1:+9389058 | MS.gene70306:CDS |
| GAATTGGGGAGACCAGTGCG+CGG | 0.788727 | 4.1:-9387364 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATAAATAGAAGAATAT+GGG | - | chr4.1:9387511-9387530 | None:intergenic | 10.0% |
| !! | ATGTATGAGTTTAAAAAAAA+TGG | + | chr4.1:9387893-9387912 | MS.gene70306:intron | 15.0% |
| !! | GATTAATAAATAGAAGAATA+TGG | - | chr4.1:9387512-9387531 | None:intergenic | 15.0% |
| !! | TGTATGAGTTTAAAAAAAAT+GGG | + | chr4.1:9387894-9387913 | MS.gene70306:intron | 15.0% |
| !! | TTAATAAATAGAAGAATATG+GGG | - | chr4.1:9387510-9387529 | None:intergenic | 15.0% |
| !!! | ATATTTTGCTTGTTTTTATT+TGG | + | chr4.1:9388465-9388484 | MS.gene70306:intron | 15.0% |
| !!! | TATTTTGCTTGTTTTTATTT+GGG | + | chr4.1:9388466-9388485 | MS.gene70306:intron | 15.0% |
| !! | AGGAAATAACAAAACATAAA+TGG | - | chr4.1:9387720-9387739 | None:intergenic | 20.0% |
| !! | ATCAAATAATTTAATCACCT+TGG | - | chr4.1:9388318-9388337 | None:intergenic | 20.0% |
| !! | CAAAATACTCCTATTAATTA+GGG | - | chr4.1:9387684-9387703 | None:intergenic | 20.0% |
| !! | CAATATCTTCAAATGAATAT+GGG | + | chr4.1:9388354-9388373 | MS.gene70306:intron | 20.0% |
| !! | TCAAAATACTCCTATTAATT+AGG | - | chr4.1:9387685-9387704 | None:intergenic | 20.0% |
| !!! | AGTTTGATAACATTTATACT+TGG | - | chr4.1:9388506-9388525 | None:intergenic | 20.0% |
| ! | AAAACCTTTGTATTCAATGA+TGG | - | chr4.1:9388235-9388254 | None:intergenic | 25.0% |
| ! | ATTACGACAATTTCATGAAT+TGG | - | chr4.1:9387575-9387594 | None:intergenic | 25.0% |
| ! | CAAGATTTGTGTTTAAGAAT+TGG | + | chr4.1:9388132-9388151 | MS.gene70306:CDS | 25.0% |
| ! | CAATGATAATAAAAACTGAG+TGG | + | chr4.1:9388533-9388552 | MS.gene70306:intron | 25.0% |
| ! | CCAATATCTTCAAATGAATA+TGG | + | chr4.1:9388353-9388372 | MS.gene70306:intron | 25.0% |
| ! | CCATATTCATTTGAAGATAT+TGG | - | chr4.1:9388356-9388375 | None:intergenic | 25.0% |
| ! | GCAAATGTTGAATAAATACA+TGG | - | chr4.1:9388005-9388024 | None:intergenic | 25.0% |
| !! | ATTAAGATTTTCATCGATAC+TGG | - | chr4.1:9388382-9388401 | None:intergenic | 25.0% |
| !! | CTTTTAGTAGTAATTAAGCT+TGG | + | chr4.1:9388879-9388898 | MS.gene70306:intron | 25.0% |
| !! | TTTTAAACTCATACATAGCA+TGG | - | chr4.1:9387889-9387908 | None:intergenic | 25.0% |
| !!! | AGGAGTATTTTGAAAGATTA+AGG | + | chr4.1:9387692-9387711 | MS.gene70306:intron | 25.0% |
| !!! | CTTTTTAAGCTTTTGTTTAC+TGG | + | chr4.1:9388793-9388812 | MS.gene70306:intron | 25.0% |
| !!! | GCAGTTCAAACTTTTTTTTA+TGG | + | chr4.1:9388842-9388861 | MS.gene70306:intron | 25.0% |
| ACAGATATGATAGTTAACAG+AGG | - | chr4.1:9388621-9388640 | None:intergenic | 30.0% | |
| ATACTCCTATTAATTAGGGT+AGG | - | chr4.1:9387680-9387699 | None:intergenic | 30.0% | |
| TCAATCACTTGAATAAGATG+AGG | + | chr4.1:9388097-9388116 | MS.gene70306:CDS | 30.0% | |
| !! | GAATACAAAGGTTTTGTCAT+TGG | + | chr4.1:9388240-9388259 | MS.gene70306:CDS | 30.0% |
| !! | TTAAGAATTGGTGTCAAACA+AGG | + | chr4.1:9388144-9388163 | MS.gene70306:CDS | 30.0% |
| !! | TTGTTATTTCCTATGAACCA+CGG | + | chr4.1:9387728-9387747 | MS.gene70306:intron | 30.0% |
| !!! | TTTTTTTTATGGCTGCACTT+GGG | + | chr4.1:9388853-9388872 | MS.gene70306:intron | 30.0% |
| AAATGATTTACCACATCCAC+AGG | - | chr4.1:9389077-9389096 | None:intergenic | 35.0% | |
| AAGAACTGAAGAAGATAACG+TGG | - | chr4.1:9388776-9388795 | None:intergenic | 35.0% | |
| ATCGAATTGAGAGAGGTTTA+GGG | - | chr4.1:9387461-9387480 | None:intergenic | 35.0% | |
| ATTAATTAGGGTAGGCAAAG+CGG | - | chr4.1:9387672-9387691 | None:intergenic | 35.0% | |
| CTTTGCCTACCCTAATTAAT+AGG | + | chr4.1:9387672-9387691 | MS.gene70306:intron | 35.0% | |
| GACTCCATCATTGAATACAA+AGG | + | chr4.1:9388228-9388247 | MS.gene70306:CDS | 35.0% | |
| GGGGTTATGCAGAAAAATTA+AGG | - | chr4.1:9387651-9387670 | None:intergenic | 35.0% | |
| TCAAGACTTGATTGAAAGCT+TGG | + | chr4.1:9388744-9388763 | MS.gene70306:intron | 35.0% | |
| TTATCTGTAAGCGAAATTGC+AGG | - | chr4.1:9388072-9388091 | None:intergenic | 35.0% | |
| ! | CTTTACACTCAGATTAGTCA+GGG | + | chr4.1:9387843-9387862 | MS.gene70306:intron | 35.0% |
| ! | TCTTTACACTCAGATTAGTC+AGG | + | chr4.1:9387842-9387861 | MS.gene70306:intron | 35.0% |
| ! | TGTTGTTGTTGTTGTACAGT+GGG | + | chr4.1:9388418-9388437 | MS.gene70306:intron | 35.0% |
| ! | TTGTTGTTGTTGTTGTACAG+TGG | + | chr4.1:9388417-9388436 | MS.gene70306:intron | 35.0% |
| ! | TTTTCCTGCTCTTTCTCTTT+TGG | + | chr4.1:9388291-9388310 | MS.gene70306:intron | 35.0% |
| !! | GTCATACACAATGGATTTTG+AGG | + | chr4.1:9388182-9388201 | MS.gene70306:CDS | 35.0% |
| !!! | CTTTTTTTTATGGCTGCACT+TGG | + | chr4.1:9388852-9388871 | MS.gene70306:intron | 35.0% |
| !!! | TTACTGGTTTTTGCAGCAAT+TGG | + | chr4.1:9388809-9388828 | MS.gene70306:intron | 35.0% |
| AACTGAAGAAGATAACGTGG+AGG | - | chr4.1:9388773-9388792 | None:intergenic | 40.0% | |
| AAGAGCCTACTCTTCGTATT+TGG | + | chr4.1:9388980-9388999 | MS.gene70306:CDS | 40.0% | |
| AATACGAAGAGTAGGCTCTT+AGG | - | chr4.1:9388980-9388999 | None:intergenic | 40.0% | |
| AATCCTGCTTAACAAAGTGC+TGG | - | chr4.1:9388710-9388729 | None:intergenic | 40.0% | |
| ACAAACCTAGCAGCAAGTAT+TGG | - | chr4.1:9387612-9387631 | None:intergenic | 40.0% | |
| CAAACCTAGCAGCAAGTATT+GGG | - | chr4.1:9387611-9387630 | None:intergenic | 40.0% | |
| CTCTGGTATGTCATACACAA+TGG | + | chr4.1:9388173-9388192 | MS.gene70306:CDS | 40.0% | |
| GAAAGTGATCGAATTGAGAG+AGG | - | chr4.1:9387468-9387487 | None:intergenic | 40.0% | |
| GAGATAAGAAATCACCCATG+TGG | - | chr4.1:9387761-9387780 | None:intergenic | 40.0% | |
| GATCGAATTGAGAGAGGTTT+AGG | - | chr4.1:9387462-9387481 | None:intergenic | 40.0% | |
| GCATTCCAAATACGAAGAGT+AGG | - | chr4.1:9388988-9389007 | None:intergenic | 40.0% | |
| TAAGCTTGGCAAACGAAAGA+AGG | + | chr4.1:9388893-9388912 | MS.gene70306:intron | 40.0% | |
| TAGAAGAATATGGGGCTTAC+CGG | - | chr4.1:9387502-9387521 | None:intergenic | 40.0% | |
| TTAATTAGGGTAGGCAAAGC+GGG | - | chr4.1:9387671-9387690 | None:intergenic | 40.0% | |
| ! | AAACCTAGCAGCAAGTATTG+GGG | - | chr4.1:9387610-9387629 | None:intergenic | 40.0% |
| ! | TATTCAATGATGGAGTCGTC+TGG | - | chr4.1:9388225-9388244 | None:intergenic | 40.0% |
| !! | ACACAATGGATTTTGAGGAC+AGG | + | chr4.1:9388187-9388206 | MS.gene70306:CDS | 40.0% |
| !! | AGAATTGGTGTCAAACAAGG+TGG | + | chr4.1:9388147-9388166 | MS.gene70306:CDS | 40.0% |
| !! | CACAATGGATTTTGAGGACA+GGG | + | chr4.1:9388188-9388207 | MS.gene70306:CDS | 40.0% |
| !! | GAATTGGTGTCAAACAAGGT+GGG | + | chr4.1:9388148-9388167 | MS.gene70306:CDS | 40.0% |
| !!! | GTGGGTGTTTTATTTCAACG+CGG | + | chr4.1:9388436-9388455 | MS.gene70306:intron | 40.0% |
| !!! | TGGGTGTTTTATTTCAACGC+GGG | + | chr4.1:9388437-9388456 | MS.gene70306:intron | 40.0% |
| AATACATGGTCGAGACAGAC+AGG | - | chr4.1:9387991-9388010 | None:intergenic | 45.0% | |
| ATACATGGTCGAGACAGACA+GGG | - | chr4.1:9387990-9388009 | None:intergenic | 45.0% | |
| ATTGGAACGCGAACGAATTG+GGG | - | chr4.1:9387381-9387400 | None:intergenic | 45.0% | |
| CTAAAGCGGCGATTGAGAAT+TGG | - | chr4.1:9387438-9387457 | None:intergenic | 45.0% | |
| GACTACAGTGATGCTCTGAT+TGG | + | chr4.1:9389013-9389032 | MS.gene70306:CDS | 45.0% | |
| GAGAGAGAGCTATGAGTGAT+TGG | - | chr4.1:9387399-9387418 | None:intergenic | 45.0% | |
| GATTGGAACGCGAACGAATT+GGG | - | chr4.1:9387382-9387401 | None:intergenic | 45.0% | |
| TAATTAGGGTAGGCAAAGCG+GGG | - | chr4.1:9387670-9387689 | None:intergenic | 45.0% | |
| TACAGTGATGCTCTGATTGG+AGG | + | chr4.1:9389016-9389035 | MS.gene70306:CDS | 45.0% | |
| TCACTTTCCGTTATTGCTGC+CGG | + | chr4.1:9387480-9387499 | MS.gene70306:CDS | 45.0% | |
| TGATTGGAACGCGAACGAAT+TGG | - | chr4.1:9387383-9387402 | None:intergenic | 45.0% | |
| TGGACCAAAAGAGAAAGAGC+AGG | - | chr4.1:9388298-9388317 | None:intergenic | 45.0% | |
| ! | AGAGAGGTTTAGGGCTAAAG+CGG | - | chr4.1:9387452-9387471 | None:intergenic | 45.0% |
| ! | GCTCTTTCTCTTTTGGTCCA+AGG | + | chr4.1:9388298-9388317 | MS.gene70306:intron | 45.0% |
| ! | TCAGGGTTCGTTGTGACTTT+TGG | + | chr4.1:9387860-9387879 | MS.gene70306:intron | 45.0% |
| !! | CTGGTTTTTGCAGCAATTGG+TGG | + | chr4.1:9388812-9388831 | MS.gene70306:intron | 45.0% |
| !! | TTTTAGCTGCACCTGCATCT+GGG | + | chr4.1:9388037-9388056 | MS.gene70306:intron | 45.0% |
| !!! | ATTTTAGCTGCACCTGCATC+TGG | + | chr4.1:9388036-9388055 | MS.gene70306:intron | 45.0% |
| AAGCGAAATTGCAGGTGCCA+CGG | - | chr4.1:9388064-9388083 | None:intergenic | 50.0% | |
| AGTGATGCTCTGATTGGAGG+AGG | + | chr4.1:9389019-9389038 | MS.gene70306:CDS | 50.0% | |
| CAATTACTACTACCGCGCAC+TGG | + | chr4.1:9387352-9387371 | MS.gene70306:CDS | 50.0% | |
| CCACAGGTTTGTGTCGCATT+AGG | - | chr4.1:9389061-9389080 | None:intergenic | 50.0% | |
| CCTAATGCGACACAAACCTG+TGG | + | chr4.1:9389058-9389077 | MS.gene70306:CDS | 50.0% | |
| CTGCCAGCACTTTGTTAAGC+AGG | + | chr4.1:9388704-9388723 | MS.gene70306:intron | 50.0% | |
| TACATGGTCGAGACAGACAG+GGG | - | chr4.1:9387989-9388008 | None:intergenic | 50.0% | |
| TTGAGGACAGGGCTAATGCA+AGG | + | chr4.1:9388199-9388218 | MS.gene70306:CDS | 50.0% | |
| TTTAGCTGCACCTGCATCTG+GGG | + | chr4.1:9388038-9388057 | MS.gene70306:intron | 50.0% | |
| ! | AGACCCCAATACTTGCTGCT+AGG | + | chr4.1:9387604-9387623 | MS.gene70306:intron | 50.0% |
| ! | GGGAGTGCAACAAAGCACTT+CGG | + | chr4.1:9387914-9387933 | MS.gene70306:intron | 50.0% |
| AACCACGGACACATGCCACA+TGG | + | chr4.1:9387743-9387762 | MS.gene70306:intron | 55.0% | |
| ACCACGGACACATGCCACAT+GGG | + | chr4.1:9387744-9387763 | MS.gene70306:intron | 55.0% | |
| AGAGAAAGCAGCACCAGCCA+TGG | - | chr4.1:9387331-9387350 | None:intergenic | 55.0% | |
| GCGACACAAACCTGTGGATG+TGG | + | chr4.1:9389064-9389083 | MS.gene70306:CDS | 55.0% | |
| GGCATGTGTCCGTGGTTCAT+AGG | - | chr4.1:9387740-9387759 | None:intergenic | 55.0% | |
| GGGCTTACCGGCAGCAATAA+CGG | - | chr4.1:9387490-9387509 | None:intergenic | 55.0% | |
| GTCAAACAAGGTGGGTGCTC+TGG | + | chr4.1:9388156-9388175 | MS.gene70306:CDS | 55.0% | |
| ACCCATGTGGCATGTGTCCG+TGG | - | chr4.1:9387748-9387767 | None:intergenic | 60.0% | |
| GAATTGGGGAGACCAGTGCG+CGG | - | chr4.1:9387367-9387386 | None:intergenic | 60.0% | |
| ! | TGCACCTGCATCTGGGGCCG+TGG | + | chr4.1:9388044-9388063 | MS.gene70306:CDS | 70.0% |
| ! | GGTGCCACGGCCCCAGATGC+AGG | - | chr4.1:9388051-9388070 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 9387324 | 9389111 | 9387324 | ID=MS.gene70306 |
| chr4.1 | mRNA | 9387324 | 9389111 | 9387324 | ID=MS.gene70306.t1;Parent=MS.gene70306 |
| chr4.1 | exon | 9387324 | 9387501 | 9387324 | ID=MS.gene70306.t1.exon1;Parent=MS.gene70306.t1 |
| chr4.1 | CDS | 9387324 | 9387501 | 9387324 | ID=cds.MS.gene70306.t1;Parent=MS.gene70306.t1 |
| chr4.1 | exon | 9388043 | 9388261 | 9388043 | ID=MS.gene70306.t1.exon2;Parent=MS.gene70306.t1 |
| chr4.1 | CDS | 9388043 | 9388261 | 9388043 | ID=cds.MS.gene70306.t1;Parent=MS.gene70306.t1 |
| chr4.1 | exon | 9388969 | 9389111 | 9388969 | ID=MS.gene70306.t1.exon3;Parent=MS.gene70306.t1 |
| chr4.1 | CDS | 9388969 | 9389111 | 9388969 | ID=cds.MS.gene70306.t1;Parent=MS.gene70306.t1 |
| Gene Sequence |
| Protein sequence |
