Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72923.t1 | PNX79454.1 | 96.6 | 118 | 4 | 0 | 1 | 118 | 14 | 131 | 2.00E-44 | 188.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72923.t1 | P14009 | 65.5 | 84 | 29 | 0 | 35 | 118 | 54 | 137 | 1.9e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72923.t1 | A0A2K3LLP0 | 96.6 | 118 | 4 | 0 | 1 | 118 | 14 | 131 | 1.4e-44 | 188.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene72923 | -0.820849 | 5.52E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene72923 | -0.834626 | 2.69E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene72923 | 0.802831 | 4.65E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene72923 | 0.806117 | 9.61E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene72923 | -0.8404 | 8.90E-58 | -1.69E-46 |
| MS.gene050170 | MS.gene72923 | -0.819703 | 1.01E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene72923 | -0.810047 | 1.40E-50 | -1.69E-46 |
| MS.gene050338 | MS.gene72923 | 0.85237 | 4.83E-61 | -1.69E-46 |
| MS.gene050444 | MS.gene72923 | -0.828346 | 9.46E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene72923 | -0.800154 | 1.65E-48 | -1.69E-46 |
| MS.gene050553 | MS.gene72923 | -0.801507 | 8.72E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene72923 | -0.845336 | 4.33E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene72923 | -0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene050710 | MS.gene72923 | 0.823479 | 1.36E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene72923 | -0.812908 | 3.35E-51 | -1.69E-46 |
| MS.gene050809 | MS.gene72923 | 0.816379 | 5.70E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene72923 | -0.83098 | 2.16E-55 | -1.69E-46 |
| MS.gene05081 | MS.gene72923 | -0.825086 | 5.68E-54 | -1.69E-46 |
| MS.gene050868 | MS.gene72923 | -0.845303 | 4.42E-59 | -1.69E-46 |
| MS.gene050871 | MS.gene72923 | -0.844906 | 5.66E-59 | -1.69E-46 |
| MS.gene050904 | MS.gene72923 | -0.826753 | 2.28E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene72923 | -0.811373 | 7.23E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene72923 | 0.821395 | 4.13E-53 | -1.69E-46 |
| MS.gene051317 | MS.gene72923 | -0.815922 | 7.21E-52 | -1.69E-46 |
| MS.gene051439 | MS.gene72923 | 0.836914 | 7.08E-57 | -1.69E-46 |
| MS.gene051517 | MS.gene72923 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene72923 | 0.820779 | 5.73E-53 | -1.69E-46 |
| MS.gene051838 | MS.gene72923 | -0.858821 | 6.33E-63 | -1.69E-46 |
| MS.gene051941 | MS.gene72923 | -0.808337 | 3.26E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene72923 | -0.806815 | 6.85E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene72923 | -0.830654 | 2.60E-55 | -1.69E-46 |
| MS.gene05240 | MS.gene72923 | 0.813366 | 2.65E-51 | -1.69E-46 |
| MS.gene052486 | MS.gene72923 | -0.800156 | 1.64E-48 | -1.69E-46 |
| MS.gene052503 | MS.gene72923 | -0.816375 | 5.71E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene72923 | 0.818468 | 1.93E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene72923 | -0.816218 | 6.19E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene72923 | -0.814128 | 1.80E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene72923 | -0.814573 | 1.44E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene72923 | -0.813365 | 2.66E-51 | -1.69E-46 |
| MS.gene053108 | MS.gene72923 | 0.809521 | 1.82E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene72923 | -0.825644 | 4.19E-54 | -1.69E-46 |
| MS.gene053479 | MS.gene72923 | -0.822728 | 2.03E-53 | -1.69E-46 |
| MS.gene053480 | MS.gene72923 | -0.823095 | 1.67E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene72923 | -0.802769 | 4.79E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene72923 | -0.829107 | 6.19E-55 | -1.69E-46 |
| MS.gene054344 | MS.gene72923 | -0.802432 | 5.63E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene72923 | -0.804285 | 2.33E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene72923 | 0.821222 | 4.53E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene72923 | -0.845 | 5.34E-59 | -1.69E-46 |
| MS.gene054742 | MS.gene72923 | 0.832604 | 8.60E-56 | -1.69E-46 |
| MS.gene054884 | MS.gene72923 | 0.839294 | 1.73E-57 | -1.69E-46 |
| MS.gene054941 | MS.gene72923 | 0.807453 | 5.02E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene72923 | -0.814792 | 1.29E-51 | -1.69E-46 |
| MS.gene054965 | MS.gene72923 | 0.800394 | 1.47E-48 | -1.69E-46 |
| MS.gene055158 | MS.gene72923 | 0.810513 | 1.11E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene72923 | -0.820495 | 6.66E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene72923 | -0.822206 | 2.68E-53 | -1.69E-46 |
| MS.gene055577 | MS.gene72923 | -0.832774 | 7.80E-56 | -1.69E-46 |
| MS.gene055729 | MS.gene72923 | 0.803568 | 3.28E-49 | -1.69E-46 |
| MS.gene055730 | MS.gene72923 | 0.802933 | 4.43E-49 | -1.69E-46 |
| MS.gene055731 | MS.gene72923 | 0.81064 | 1.04E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene72923 | -0.844411 | 7.69E-59 | -1.69E-46 |
| MS.gene055750 | MS.gene72923 | -0.833956 | 3.96E-56 | -1.69E-46 |
| MS.gene055751 | MS.gene72923 | -0.842002 | 3.37E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene72923 | -0.864779 | 9.51E-65 | -1.69E-46 |
| MS.gene055941 | MS.gene72923 | -0.818285 | 2.12E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene72923 | -0.807517 | 4.86E-50 | -1.69E-46 |
| MS.gene056283 | MS.gene72923 | -0.828083 | 1.10E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene72923 | -0.859989 | 2.82E-63 | -1.69E-46 |
| MS.gene056504 | MS.gene72923 | -0.834683 | 2.60E-56 | -1.69E-46 |
| MS.gene056590 | MS.gene72923 | 0.814063 | 1.86E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene72923 | 0.812388 | 4.35E-51 | -1.69E-46 |
| MS.gene056618 | MS.gene72923 | 0.821434 | 4.05E-53 | -1.69E-46 |
| MS.gene056688 | MS.gene72923 | 0.803076 | 4.14E-49 | -1.69E-46 |
| MS.gene056692 | MS.gene72923 | -0.821654 | 3.60E-53 | -1.69E-46 |
| MS.gene056694 | MS.gene72923 | 0.830432 | 2.95E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene72923 | 0.844454 | 7.49E-59 | -1.69E-46 |
| MS.gene056856 | MS.gene72923 | 0.822867 | 1.88E-53 | -1.69E-46 |
| MS.gene057086 | MS.gene72923 | -0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene057332 | MS.gene72923 | -0.810472 | 1.13E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene72923 | -0.833335 | 5.66E-56 | -1.69E-46 |
| MS.gene057717 | MS.gene72923 | 0.83184 | 1.33E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene72923 | -0.80583 | 1.10E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene72923 | -0.809465 | 1.87E-50 | -1.69E-46 |
| MS.gene057856 | MS.gene72923 | -0.820392 | 7.03E-53 | -1.69E-46 |
| MS.gene057857 | MS.gene72923 | -0.830249 | 3.27E-55 | -1.69E-46 |
| MS.gene058158 | MS.gene72923 | 0.800776 | 1.23E-48 | -1.69E-46 |
| MS.gene058334 | MS.gene72923 | 0.810048 | 1.40E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene72923 | -0.804832 | 1.79E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene72923 | -0.812679 | 3.75E-51 | -1.69E-46 |
| MS.gene058418 | MS.gene72923 | 0.808837 | 2.55E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene72923 | -0.80158 | 8.42E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene72923 | -0.810678 | 1.02E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene72923 | 0.818108 | 2.33E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene72923 | -0.839107 | 1.93E-57 | -1.69E-46 |
| MS.gene059023 | MS.gene72923 | -0.82957 | 4.78E-55 | -1.69E-46 |
| MS.gene05914 | MS.gene72923 | -0.833577 | 4.92E-56 | -1.69E-46 |
| MS.gene05926 | MS.gene72923 | 0.861829 | 7.79E-64 | -1.69E-46 |
| MS.gene05934 | MS.gene72923 | -0.815365 | 9.59E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene72923 | 0.851679 | 7.59E-61 | -1.69E-46 |
| MS.gene059667 | MS.gene72923 | -0.837264 | 5.76E-57 | -1.69E-46 |
| MS.gene059865 | MS.gene72923 | -0.816128 | 6.49E-52 | -1.69E-46 |
| MS.gene06000 | MS.gene72923 | -0.854932 | 8.86E-62 | -1.69E-46 |
| MS.gene060087 | MS.gene72923 | 0.815052 | 1.13E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene72923 | 0.840508 | 8.34E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene72923 | -0.848193 | 7.17E-60 | -1.69E-46 |
| MS.gene06077 | MS.gene72923 | -0.809616 | 1.73E-50 | -1.69E-46 |
| MS.gene060823 | MS.gene72923 | 0.804923 | 1.71E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene72923 | -0.838256 | 3.20E-57 | -1.69E-46 |
| MS.gene061044 | MS.gene72923 | 0.820542 | 6.49E-53 | -1.69E-46 |
| MS.gene061231 | MS.gene72923 | -0.81505 | 1.13E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72923.t1 | MTR_4g101310 | 96.610 | 118 | 4 | 0 | 1 | 118 | 17 | 134 | 3.59e-78 | 226 |
| MS.gene72923.t1 | MTR_4g101330 | 50.000 | 152 | 41 | 2 | 1 | 117 | 17 | 168 | 1.18e-39 | 130 |
| MS.gene72923.t1 | MTR_4g101280 | 67.045 | 88 | 29 | 0 | 31 | 118 | 56 | 143 | 8.13e-38 | 125 |
| MS.gene72923.t1 | MTR_1g012710 | 61.628 | 86 | 32 | 1 | 33 | 118 | 67 | 151 | 1.55e-34 | 117 |
| MS.gene72923.t1 | MTR_1g012690 | 56.190 | 105 | 37 | 3 | 14 | 118 | 59 | 154 | 1.60e-34 | 117 |
| MS.gene72923.t1 | MTR_1g012700 | 50.442 | 113 | 47 | 2 | 14 | 118 | 53 | 164 | 1.92e-32 | 112 |
| MS.gene72923.t1 | MTR_4g108150 | 48.739 | 119 | 52 | 3 | 1 | 118 | 13 | 123 | 1.93e-31 | 108 |
| MS.gene72923.t1 | MTR_2g026760 | 52.941 | 119 | 49 | 5 | 1 | 118 | 15 | 127 | 2.29e-31 | 108 |
| MS.gene72923.t1 | MTR_3g106740 | 62.353 | 85 | 30 | 2 | 34 | 117 | 46 | 129 | 3.35e-31 | 107 |
| MS.gene72923.t1 | MTR_1g012630 | 58.140 | 86 | 35 | 1 | 33 | 118 | 126 | 210 | 6.99e-31 | 109 |
| MS.gene72923.t1 | MTR_4g101260 | 49.587 | 121 | 57 | 3 | 1 | 118 | 13 | 132 | 6.44e-30 | 104 |
| MS.gene72923.t1 | MTR_5g012340 | 50.575 | 87 | 42 | 1 | 31 | 117 | 91 | 176 | 4.91e-26 | 96.3 |
| MS.gene72923.t1 | MTR_5g012380 | 50.575 | 87 | 42 | 1 | 31 | 117 | 84 | 169 | 6.80e-26 | 95.5 |
| MS.gene72923.t1 | MTR_3g106750 | 54.762 | 84 | 38 | 0 | 35 | 118 | 46 | 129 | 2.32e-25 | 92.8 |
| MS.gene72923.t1 | MTR_4g101270 | 51.765 | 85 | 40 | 1 | 33 | 117 | 103 | 186 | 3.25e-24 | 91.7 |
| MS.gene72923.t1 | MTR_2g026920 | 44.444 | 81 | 42 | 1 | 38 | 118 | 118 | 195 | 6.28e-17 | 72.8 |
| MS.gene72923.t1 | MTR_2g026930 | 44.444 | 81 | 42 | 1 | 38 | 118 | 122 | 199 | 8.43e-17 | 72.8 |
| MS.gene72923.t1 | MTR_3g082100 | 45.977 | 87 | 46 | 1 | 31 | 117 | 250 | 335 | 5.08e-16 | 72.4 |
| MS.gene72923.t1 | MTR_2g026775 | 48.101 | 79 | 35 | 2 | 42 | 118 | 686 | 760 | 4.34e-14 | 67.4 |
| MS.gene72923.t1 | MTR_2g026820 | 49.367 | 79 | 34 | 3 | 40 | 117 | 542 | 615 | 9.69e-14 | 66.2 |
| MS.gene72923.t1 | MTR_4g115360 | 44.444 | 90 | 49 | 1 | 29 | 118 | 145 | 233 | 1.40e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72923.t1 | AT1G12100 | 55.932 | 118 | 50 | 1 | 1 | 118 | 17 | 132 | 7.54e-39 | 127 |
| MS.gene72923.t1 | AT1G62510 | 66.667 | 84 | 28 | 0 | 34 | 117 | 66 | 149 | 5.10e-36 | 120 |
| MS.gene72923.t1 | AT4G12500 | 57.955 | 88 | 37 | 0 | 31 | 118 | 90 | 177 | 1.50e-34 | 117 |
| MS.gene72923.t1 | AT4G12520 | 50.000 | 118 | 54 | 1 | 1 | 118 | 17 | 129 | 3.40e-34 | 115 |
| MS.gene72923.t1 | AT4G12510 | 50.000 | 118 | 54 | 1 | 1 | 118 | 17 | 129 | 3.40e-34 | 115 |
| MS.gene72923.t1 | AT4G12490 | 56.989 | 93 | 40 | 0 | 26 | 118 | 90 | 182 | 9.28e-34 | 115 |
| MS.gene72923.t1 | AT2G45180 | 63.529 | 85 | 30 | 1 | 34 | 118 | 51 | 134 | 2.38e-33 | 113 |
| MS.gene72923.t1 | AT4G22460 | 53.571 | 112 | 51 | 1 | 5 | 116 | 21 | 131 | 2.91e-33 | 113 |
| MS.gene72923.t1 | AT1G12090 | 65.432 | 81 | 28 | 0 | 37 | 117 | 57 | 137 | 1.64e-32 | 111 |
| MS.gene72923.t1 | AT4G12480 | 55.682 | 88 | 39 | 0 | 31 | 118 | 81 | 168 | 2.03e-32 | 112 |
| MS.gene72923.t1 | AT4G12470 | 55.056 | 89 | 40 | 0 | 30 | 118 | 73 | 161 | 3.59e-32 | 111 |
| MS.gene72923.t1 | AT4G12550 | 49.495 | 99 | 48 | 2 | 21 | 118 | 14 | 111 | 2.54e-29 | 102 |
| MS.gene72923.t1 | AT5G46890 | 47.458 | 118 | 58 | 2 | 1 | 118 | 14 | 127 | 6.86e-28 | 99.4 |
| MS.gene72923.t1 | AT4G00165 | 48.544 | 103 | 51 | 2 | 16 | 117 | 27 | 128 | 1.37e-27 | 98.6 |
| MS.gene72923.t1 | AT4G00165 | 48.544 | 103 | 51 | 2 | 16 | 117 | 27 | 128 | 1.37e-27 | 98.6 |
| MS.gene72923.t1 | AT4G12530 | 54.945 | 91 | 40 | 1 | 27 | 117 | 26 | 115 | 1.03e-26 | 95.9 |
| MS.gene72923.t1 | AT5G46900 | 54.444 | 90 | 38 | 1 | 29 | 118 | 41 | 127 | 1.03e-26 | 96.3 |
| MS.gene72923.t1 | AT4G12545 | 49.495 | 99 | 45 | 3 | 21 | 118 | 14 | 108 | 6.29e-26 | 94.0 |
| MS.gene72923.t1 | AT3G22120 | 50.000 | 86 | 41 | 2 | 33 | 117 | 265 | 349 | 2.22e-19 | 81.6 |
| MS.gene72923.t1 | AT3G22120 | 50.000 | 86 | 41 | 2 | 33 | 117 | 248 | 332 | 3.34e-19 | 81.3 |
| MS.gene72923.t1 | AT3G22142 | 50.538 | 93 | 43 | 2 | 27 | 117 | 1387 | 1478 | 3.23e-18 | 79.3 |
| MS.gene72923.t1 | AT1G62500 | 46.429 | 84 | 44 | 1 | 34 | 117 | 211 | 293 | 9.61e-18 | 76.6 |
| MS.gene72923.t1 | AT2G10940 | 44.706 | 85 | 46 | 1 | 34 | 118 | 207 | 290 | 1.29e-17 | 76.3 |
| MS.gene72923.t1 | AT2G10940 | 44.706 | 85 | 46 | 1 | 34 | 118 | 207 | 290 | 1.29e-17 | 76.3 |
| MS.gene72923.t1 | AT4G15160 | 44.186 | 86 | 47 | 1 | 33 | 117 | 97 | 182 | 1.05e-16 | 72.4 |
| MS.gene72923.t1 | AT4G15160 | 44.186 | 86 | 47 | 1 | 33 | 117 | 179 | 264 | 3.10e-16 | 72.4 |
| MS.gene72923.t1 | AT4G22490 | 35.870 | 92 | 56 | 2 | 26 | 117 | 28 | 116 | 1.10e-11 | 57.8 |
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGATCAAACTTAGTGAAAT+AGG | 0.251033 | 8.3:-19976612 | None:intergenic |
| TTTGGATTAGGGTTTGGTTT+TGG | 0.251880 | 8.3:-19976363 | None:intergenic |
| GTGTGTGCTAATTTGCTTAA+TGG | 0.265220 | 8.3:+19976453 | MS.gene72923:CDS |
| AAGGGAATGGATTTGGATTA+GGG | 0.269618 | 8.3:-19976374 | None:intergenic |
| GCCAAGAATGTTAGCTTTGA+TGG | 0.271570 | 8.3:-19976574 | None:intergenic |
| CTTGGGTAAGGGAATGGATT+TGG | 0.286724 | 8.3:-19976381 | None:intergenic |
| TCCAAGAGATGCACTCAAAT+TGG | 0.300623 | 8.3:+19976428 | MS.gene72923:CDS |
| TAAGGGAATGGATTTGGATT+AGG | 0.323838 | 8.3:-19976375 | None:intergenic |
| GCCATCAAAGCTAACATTCT+TGG | 0.340537 | 8.3:+19976573 | MS.gene72923:CDS |
| ATAGAGCAACATGGATGTTC+TGG | 0.348478 | 8.3:-19976504 | None:intergenic |
| CCCAATTTGAGTGCATCTCT+TGG | 0.362878 | 8.3:-19976429 | None:intergenic |
| GAGTCTTCTCACAAGCATTA+AGG | 0.369143 | 8.3:-19976632 | None:intergenic |
| AATGGATTTGGATTAGGGTT+TGG | 0.380389 | 8.3:-19976369 | None:intergenic |
| GAAGGACTTGTGCTTGGGTA+AGG | 0.431525 | 8.3:-19976393 | None:intergenic |
| GGACCAATTGGTGCAGTGAT+TGG | 0.437596 | 8.3:+19976474 | MS.gene72923:CDS |
| CCAAGAGATGCACTCAAATT+GGG | 0.438012 | 8.3:+19976429 | MS.gene72923:CDS |
| GACCCAATCACTGCACCAAT+TGG | 0.459457 | 8.3:-19976477 | None:intergenic |
| TAGTGGAAGGACTTGTGCTT+GGG | 0.461750 | 8.3:-19976398 | None:intergenic |
| AATTTGCTTAATGGACCAAT+TGG | 0.477291 | 8.3:+19976462 | MS.gene72923:CDS |
| ATCTCTTGGACAACTCTTAG+TGG | 0.495008 | 8.3:-19976415 | None:intergenic |
| TTAGTGGAAGGACTTGTGCT+TGG | 0.502701 | 8.3:-19976399 | None:intergenic |
| CTTGTGCTTGGGTAAGGGAA+TGG | 0.504980 | 8.3:-19976387 | None:intergenic |
| GACCAATTGGTGCAGTGATT+GGG | 0.509536 | 8.3:+19976475 | MS.gene72923:CDS |
| TAACACTTAATTAAGAGCAC+TGG | 0.528966 | 8.3:-19976668 | None:intergenic |
| AAGGACTTGTGCTTGGGTAA+GGG | 0.591360 | 8.3:-19976392 | None:intergenic |
| TAAGAGCACTGGAAATCAGT+GGG | 0.619022 | 8.3:-19976657 | None:intergenic |
| CTTGGACAACTCTTAGTGGA+AGG | 0.619262 | 8.3:-19976411 | None:intergenic |
| CCTTCTAAAATAGAGCAACA+TGG | 0.620844 | 8.3:-19976513 | None:intergenic |
| TTAAGAGCACTGGAAATCAG+TGG | 0.653235 | 8.3:-19976658 | None:intergenic |
| GAGCACTGGAAATCAGTGGG+TGG | 0.693320 | 8.3:-19976654 | None:intergenic |
| GAGCAACATGGATGTTCTGG+TGG | 0.695269 | 8.3:-19976501 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGATCAAACTTAGTGAAAT+AGG | - | chr8.3:19976615-19976634 | None:intergenic | 30.0% | |
| ! | AATTTGCTTAATGGACCAAT+TGG | + | chr8.3:19976462-19976481 | MS.gene72923:CDS | 30.0% |
| ! | CTTTTCTTTACAATGGCAAA+TGG | + | chr8.3:19976327-19976346 | MS.gene72923:CDS | 30.0% |
| ! | AAGGGAATGGATTTGGATTA+GGG | - | chr8.3:19976377-19976396 | None:intergenic | 35.0% |
| ! | AATGGATTTGGATTAGGGTT+TGG | - | chr8.3:19976372-19976391 | None:intergenic | 35.0% |
| ! | TAAGGGAATGGATTTGGATT+AGG | - | chr8.3:19976378-19976397 | None:intergenic | 35.0% |
| !! | CCATGTTGCTCTATTTTAGA+AGG | + | chr8.3:19976513-19976532 | MS.gene72923:CDS | 35.0% |
| !! | CCTTCTAAAATAGAGCAACA+TGG | - | chr8.3:19976516-19976535 | None:intergenic | 35.0% |
| !! | GTGTGTGCTAATTTGCTTAA+TGG | + | chr8.3:19976453-19976472 | MS.gene72923:CDS | 35.0% |
| !! | TTTGGATTAGGGTTTGGTTT+TGG | - | chr8.3:19976366-19976385 | None:intergenic | 35.0% |
| ATAGAGCAACATGGATGTTC+TGG | - | chr8.3:19976507-19976526 | None:intergenic | 40.0% | |
| ATCTCTTGGACAACTCTTAG+TGG | - | chr8.3:19976418-19976437 | None:intergenic | 40.0% | |
| CCAAGAGATGCACTCAAATT+GGG | + | chr8.3:19976429-19976448 | MS.gene72923:CDS | 40.0% | |
| GAGTCTTCTCACAAGCATTA+AGG | - | chr8.3:19976635-19976654 | None:intergenic | 40.0% | |
| GCCATCAAAGCTAACATTCT+TGG | + | chr8.3:19976573-19976592 | MS.gene72923:CDS | 40.0% | |
| TCCAAGAGATGCACTCAAAT+TGG | + | chr8.3:19976428-19976447 | MS.gene72923:CDS | 40.0% | |
| !! | GCCAAGAATGTTAGCTTTGA+TGG | - | chr8.3:19976577-19976596 | None:intergenic | 40.0% |
| !! | TAAGAGCACTGGAAATCAGT+GGG | - | chr8.3:19976660-19976679 | None:intergenic | 40.0% |
| !! | TTAAGAGCACTGGAAATCAG+TGG | - | chr8.3:19976661-19976680 | None:intergenic | 40.0% |
| AAGGACTTGTGCTTGGGTAA+GGG | - | chr8.3:19976395-19976414 | None:intergenic | 45.0% | |
| CCCAATTTGAGTGCATCTCT+TGG | - | chr8.3:19976432-19976451 | None:intergenic | 45.0% | |
| CTTGGACAACTCTTAGTGGA+AGG | - | chr8.3:19976414-19976433 | None:intergenic | 45.0% | |
| GACCAATTGGTGCAGTGATT+GGG | + | chr8.3:19976475-19976494 | MS.gene72923:CDS | 45.0% | |
| TAGTGGAAGGACTTGTGCTT+GGG | - | chr8.3:19976401-19976420 | None:intergenic | 45.0% | |
| TTAGTGGAAGGACTTGTGCT+TGG | - | chr8.3:19976402-19976421 | None:intergenic | 45.0% | |
| ! | CTTGGGTAAGGGAATGGATT+TGG | - | chr8.3:19976384-19976403 | None:intergenic | 45.0% |
| CTTGTGCTTGGGTAAGGGAA+TGG | - | chr8.3:19976390-19976409 | None:intergenic | 50.0% | |
| GAAGGACTTGTGCTTGGGTA+AGG | - | chr8.3:19976396-19976415 | None:intergenic | 50.0% | |
| GACCCAATCACTGCACCAAT+TGG | - | chr8.3:19976480-19976499 | None:intergenic | 50.0% | |
| GAGCAACATGGATGTTCTGG+TGG | - | chr8.3:19976504-19976523 | None:intergenic | 50.0% | |
| GGACCAATTGGTGCAGTGAT+TGG | + | chr8.3:19976474-19976493 | MS.gene72923:CDS | 50.0% | |
| !! | GAGCACTGGAAATCAGTGGG+TGG | - | chr8.3:19976657-19976676 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19976324 | 19976680 | 19976324 | ID=MS.gene72923 |
| chr8.3 | mRNA | 19976324 | 19976680 | 19976324 | ID=MS.gene72923.t1;Parent=MS.gene72923 |
| chr8.3 | exon | 19976324 | 19976680 | 19976324 | ID=MS.gene72923.t1.exon1;Parent=MS.gene72923.t1 |
| chr8.3 | CDS | 19976324 | 19976680 | 19976324 | ID=cds.MS.gene72923.t1;Parent=MS.gene72923.t1 |
| Gene Sequence |
| Protein sequence |
