Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21274.t1 | XP_003615366.1 | 99.4 | 507 | 3 | 0 | 1 | 507 | 1 | 507 | 1.30E-289 | 1005 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21274.t1 | P34899 | 84.7 | 516 | 70 | 2 | 1 | 507 | 3 | 518 | 2.9e-250 | 865.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21274.t1 | G7K5Z4 | 99.4 | 507 | 3 | 0 | 1 | 507 | 1 | 507 | 9.1e-290 | 1005.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene21274 | -0.847869 | 8.80E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene21274 | -0.812362 | 4.40E-51 | -1.69E-46 |
| MS.gene049251 | MS.gene21274 | 0.809217 | 2.11E-50 | -1.69E-46 |
| MS.gene04932 | MS.gene21274 | 0.807341 | 5.30E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene21274 | -0.820908 | 5.35E-53 | -1.69E-46 |
| MS.gene050170 | MS.gene21274 | -0.827439 | 1.56E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene21274 | -0.822906 | 1.84E-53 | -1.69E-46 |
| MS.gene050338 | MS.gene21274 | 0.860916 | 1.48E-63 | -1.69E-46 |
| MS.gene050444 | MS.gene21274 | -0.824558 | 7.56E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene21274 | -0.805301 | 1.43E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene21274 | -0.8129 | 3.36E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene21274 | -0.827216 | 1.77E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene21274 | -0.81868 | 1.73E-52 | -1.69E-46 |
| MS.gene050710 | MS.gene21274 | 0.838504 | 2.76E-57 | -1.69E-46 |
| MS.gene050747 | MS.gene21274 | -0.805603 | 1.23E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene21274 | 0.849227 | 3.70E-60 | -1.69E-46 |
| MS.gene05080 | MS.gene21274 | -0.835364 | 1.75E-56 | -1.69E-46 |
| MS.gene05081 | MS.gene21274 | -0.827363 | 1.63E-54 | -1.69E-46 |
| MS.gene050868 | MS.gene21274 | -0.838753 | 2.38E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene21274 | -0.836972 | 6.84E-57 | -1.69E-46 |
| MS.gene050904 | MS.gene21274 | -0.802685 | 4.99E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene21274 | 0.809009 | 2.34E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene21274 | 0.808537 | 2.95E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene21274 | 0.842816 | 2.05E-58 | -1.69E-46 |
| MS.gene051317 | MS.gene21274 | -0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene051439 | MS.gene21274 | 0.808444 | 3.09E-50 | -1.69E-46 |
| MS.gene051440 | MS.gene21274 | 0.820719 | 5.91E-53 | -1.69E-46 |
| MS.gene051517 | MS.gene21274 | 0.829245 | 5.73E-55 | -1.69E-46 |
| MS.gene051552 | MS.gene21274 | 0.812289 | 4.57E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene21274 | 0.852464 | 4.54E-61 | -1.69E-46 |
| MS.gene051749 | MS.gene21274 | 0.846883 | 1.64E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene21274 | -0.85011 | 2.10E-60 | -1.69E-46 |
| MS.gene051907 | MS.gene21274 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene21274 | 0.810381 | 1.19E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene21274 | -0.813174 | 2.93E-51 | -1.69E-46 |
| MS.gene052129 | MS.gene21274 | 0.840346 | 9.19E-58 | -1.69E-46 |
| MS.gene052164 | MS.gene21274 | -0.825927 | 3.59E-54 | -1.69E-46 |
| MS.gene05240 | MS.gene21274 | 0.844383 | 7.82E-59 | -1.69E-46 |
| MS.gene052494 | MS.gene21274 | 0.817233 | 3.67E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene21274 | -0.807781 | 4.27E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene21274 | 0.84821 | 7.08E-60 | -1.69E-46 |
| MS.gene052702 | MS.gene21274 | 0.818079 | 2.36E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene21274 | -0.810111 | 1.36E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene21274 | -0.811446 | 6.97E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene21274 | -0.811661 | 6.26E-51 | -1.69E-46 |
| MS.gene053097 | MS.gene21274 | 0.80773 | 4.38E-50 | -1.69E-46 |
| MS.gene053108 | MS.gene21274 | 0.826705 | 2.34E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene21274 | -0.817706 | 2.87E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene21274 | -0.809262 | 2.06E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene21274 | -0.806736 | 7.12E-50 | -1.69E-46 |
| MS.gene053776 | MS.gene21274 | 0.800228 | 1.59E-48 | -1.69E-46 |
| MS.gene053824 | MS.gene21274 | -0.829398 | 5.26E-55 | -1.69E-46 |
| MS.gene054290 | MS.gene21274 | 0.804646 | 1.95E-49 | -1.69E-46 |
| MS.gene054344 | MS.gene21274 | -0.834317 | 3.21E-56 | -1.69E-46 |
| MS.gene054706 | MS.gene21274 | 0.845437 | 4.06E-59 | -1.69E-46 |
| MS.gene05470 | MS.gene21274 | -0.85181 | 6.96E-61 | -1.69E-46 |
| MS.gene054742 | MS.gene21274 | 0.821445 | 4.02E-53 | -1.69E-46 |
| MS.gene054884 | MS.gene21274 | 0.851588 | 8.05E-61 | -1.69E-46 |
| MS.gene054941 | MS.gene21274 | 0.803155 | 3.99E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene21274 | -0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene055159 | MS.gene21274 | 0.800417 | 1.46E-48 | -1.69E-46 |
| MS.gene055206 | MS.gene21274 | -0.802011 | 6.87E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene21274 | -0.821209 | 4.56E-53 | -1.69E-46 |
| MS.gene055577 | MS.gene21274 | -0.835184 | 1.95E-56 | -1.69E-46 |
| MS.gene055616 | MS.gene21274 | 0.801108 | 1.05E-48 | -1.69E-46 |
| MS.gene055729 | MS.gene21274 | 0.836183 | 1.09E-56 | -1.69E-46 |
| MS.gene055730 | MS.gene21274 | 0.842216 | 2.96E-58 | -1.69E-46 |
| MS.gene055731 | MS.gene21274 | 0.856002 | 4.32E-62 | -1.69E-46 |
| MS.gene055748 | MS.gene21274 | -0.868072 | 8.57E-66 | -1.69E-46 |
| MS.gene055750 | MS.gene21274 | -0.829135 | 6.10E-55 | -1.69E-46 |
| MS.gene055751 | MS.gene21274 | -0.854088 | 1.55E-61 | -1.69E-46 |
| MS.gene05589 | MS.gene21274 | -0.862347 | 5.40E-64 | -1.69E-46 |
| MS.gene056224 | MS.gene21274 | 0.800745 | 1.25E-48 | -1.69E-46 |
| MS.gene056283 | MS.gene21274 | -0.820872 | 5.45E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene21274 | -0.84502 | 5.27E-59 | -1.69E-46 |
| MS.gene056504 | MS.gene21274 | -0.828632 | 8.07E-55 | -1.69E-46 |
| MS.gene056590 | MS.gene21274 | 0.819236 | 1.29E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene21274 | 0.820199 | 7.78E-53 | -1.69E-46 |
| MS.gene056678 | MS.gene21274 | 0.840636 | 7.72E-58 | -1.69E-46 |
| MS.gene056688 | MS.gene21274 | 0.823183 | 1.59E-53 | -1.69E-46 |
| MS.gene056694 | MS.gene21274 | 0.818236 | 2.18E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene21274 | 0.850821 | 1.33E-60 | -1.69E-46 |
| MS.gene056748 | MS.gene21274 | 0.811352 | 7.31E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene21274 | 0.864429 | 1.22E-64 | -1.69E-46 |
| MS.gene056857 | MS.gene21274 | 0.805619 | 1.22E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene21274 | 0.812701 | 3.71E-51 | -1.69E-46 |
| MS.gene057658 | MS.gene21274 | -0.839763 | 1.30E-57 | -1.69E-46 |
| MS.gene057717 | MS.gene21274 | 0.82879 | 7.39E-55 | -1.69E-46 |
| MS.gene057768 | MS.gene21274 | 0.825909 | 3.63E-54 | -1.69E-46 |
| MS.gene058158 | MS.gene21274 | 0.854832 | 9.47E-62 | -1.69E-46 |
| MS.gene058334 | MS.gene21274 | 0.832039 | 1.19E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene21274 | -0.814024 | 1.90E-51 | -1.69E-46 |
| MS.gene058382 | MS.gene21274 | 0.817491 | 3.21E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene21274 | 0.839332 | 1.69E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene21274 | -0.802165 | 6.39E-49 | -1.69E-46 |
| MS.gene058452 | MS.gene21274 | 0.806594 | 7.62E-50 | -1.69E-46 |
| MS.gene058544 | MS.gene21274 | -0.835529 | 1.59E-56 | -1.69E-46 |
| MS.gene058603 | MS.gene21274 | 0.835692 | 1.45E-56 | -1.69E-46 |
| MS.gene058838 | MS.gene21274 | -0.860317 | 2.25E-63 | -1.69E-46 |
| MS.gene059023 | MS.gene21274 | -0.840064 | 1.09E-57 | -1.69E-46 |
| MS.gene05914 | MS.gene21274 | -0.858259 | 9.31E-63 | -1.69E-46 |
| MS.gene05926 | MS.gene21274 | 0.86805 | 8.71E-66 | -1.69E-46 |
| MS.gene059304 | MS.gene21274 | 0.807623 | 4.62E-50 | -1.69E-46 |
| MS.gene059315 | MS.gene21274 | 0.832109 | 1.14E-55 | -1.69E-46 |
| MS.gene059526 | MS.gene21274 | 0.811246 | 7.71E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene21274 | 0.848496 | 5.91E-60 | -1.69E-46 |
| MS.gene059667 | MS.gene21274 | -0.832647 | 8.39E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene21274 | -0.805979 | 1.03E-49 | -1.69E-46 |
| MS.gene059699 | MS.gene21274 | 0.807913 | 4.01E-50 | -1.69E-46 |
| MS.gene059703 | MS.gene21274 | 0.818466 | 1.93E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene21274 | -0.810237 | 1.27E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene21274 | -0.803678 | 3.11E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene21274 | -0.829061 | 6.36E-55 | -1.69E-46 |
| MS.gene060087 | MS.gene21274 | 0.817228 | 3.68E-52 | -1.69E-46 |
| MS.gene06027 | MS.gene21274 | -0.823932 | 1.06E-53 | -1.69E-46 |
| MS.gene060405 | MS.gene21274 | 0.836167 | 1.10E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene21274 | -0.830306 | 3.16E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene21274 | -0.801629 | 8.23E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene21274 | -0.821548 | 3.81E-53 | -1.69E-46 |
| MS.gene061044 | MS.gene21274 | 0.839711 | 1.35E-57 | -1.69E-46 |
| MS.gene061231 | MS.gene21274 | -0.810944 | 8.96E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21274.t1 | MTR_5g067060 | 99.408 | 507 | 3 | 0 | 1 | 507 | 1 | 507 | 0.0 | 1050 |
| MS.gene21274.t1 | MTR_5g030950 | 83.622 | 519 | 70 | 2 | 1 | 507 | 3 | 518 | 0.0 | 896 |
| MS.gene21274.t1 | MTR_2g018290 | 59.430 | 456 | 172 | 3 | 45 | 497 | 87 | 532 | 0.0 | 568 |
| MS.gene21274.t1 | MTR_8g081510 | 58.120 | 468 | 187 | 5 | 42 | 506 | 9 | 470 | 0.0 | 557 |
| MS.gene21274.t1 | MTR_3g084310 | 57.692 | 468 | 189 | 5 | 42 | 506 | 9 | 470 | 0.0 | 551 |
| MS.gene21274.t1 | MTR_2g006570 | 53.219 | 466 | 206 | 5 | 42 | 500 | 133 | 593 | 3.19e-172 | 498 |
| MS.gene21274.t1 | MTR_3g073470 | 52.586 | 464 | 205 | 5 | 42 | 498 | 120 | 575 | 4.48e-170 | 492 |
| MS.gene21274.t1 | MTR_3g084310 | 62.890 | 353 | 127 | 3 | 42 | 391 | 9 | 360 | 1.19e-159 | 458 |
| MS.gene21274.t1 | MTR_2g006570 | 52.693 | 427 | 190 | 5 | 81 | 500 | 1 | 422 | 1.60e-156 | 452 |
| MS.gene21274.t1 | MTR_7g108420 | 51.034 | 435 | 177 | 6 | 72 | 498 | 3 | 409 | 1.35e-146 | 426 |
| MS.gene21274.t1 | MTR_1g073580 | 39.583 | 144 | 86 | 1 | 348 | 490 | 83 | 226 | 4.60e-26 | 107 |
| MS.gene21274.t1 | MTR_1g073580 | 52.273 | 88 | 37 | 2 | 189 | 276 | 1 | 83 | 3.64e-19 | 87.4 |
| MS.gene21274.t1 | MTR_3g062030 | 56.000 | 100 | 28 | 3 | 285 | 382 | 1 | 86 | 1.33e-24 | 102 |
| MS.gene21274.t1 | MTR_7g070020 | 54.878 | 82 | 27 | 2 | 367 | 441 | 1 | 79 | 1.51e-21 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21274.t1 | AT5G26780 | 83.366 | 517 | 76 | 2 | 1 | 507 | 1 | 517 | 0.0 | 900 |
| MS.gene21274.t1 | AT5G26780 | 83.366 | 517 | 76 | 2 | 1 | 507 | 1 | 517 | 0.0 | 900 |
| MS.gene21274.t1 | AT4G37930 | 84.241 | 514 | 73 | 2 | 1 | 506 | 3 | 516 | 0.0 | 896 |
| MS.gene21274.t1 | AT5G26780 | 81.051 | 533 | 75 | 3 | 1 | 507 | 1 | 533 | 0.0 | 892 |
| MS.gene21274.t1 | AT5G26780 | 81.051 | 533 | 75 | 3 | 1 | 507 | 1 | 533 | 0.0 | 892 |
| MS.gene21274.t1 | AT4G32520 | 61.233 | 454 | 167 | 3 | 45 | 497 | 83 | 528 | 0.0 | 587 |
| MS.gene21274.t1 | AT4G32520 | 61.233 | 454 | 167 | 3 | 45 | 497 | 83 | 528 | 0.0 | 587 |
| MS.gene21274.t1 | AT4G13930 | 57.692 | 468 | 189 | 5 | 42 | 506 | 9 | 470 | 0.0 | 545 |
| MS.gene21274.t1 | AT4G13890 | 55.628 | 462 | 196 | 5 | 42 | 500 | 9 | 464 | 0.0 | 523 |
| MS.gene21274.t1 | AT4G13890 | 59.375 | 384 | 152 | 3 | 42 | 422 | 9 | 391 | 6.73e-166 | 475 |
| MS.gene21274.t1 | AT1G22020 | 50.107 | 467 | 219 | 5 | 42 | 500 | 139 | 599 | 3.40e-163 | 476 |
| MS.gene21274.t1 | AT1G22020 | 50.107 | 467 | 219 | 5 | 42 | 500 | 139 | 599 | 3.40e-163 | 476 |
| MS.gene21274.t1 | AT1G36370 | 51.087 | 460 | 211 | 5 | 42 | 493 | 135 | 588 | 8.85e-163 | 474 |
Find 118 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTACTAAGAACTTGTTTC+TGG | 0.199826 | 5.1:-51976994 | None:intergenic |
| GCACCGGCAACTATTAATTT+TGG | 0.206093 | 5.1:-51975436 | None:intergenic |
| CAAAATAGCCTTACAAATTA+AGG | 0.250232 | 5.1:+51978135 | MS.gene21274:CDS |
| AAGCGTGCATTGGAAGCTTT+TGG | 0.259016 | 5.1:+51970824 | MS.gene21274:CDS |
| GGCAGTTATGTTGGCTGATA+TGG | 0.263270 | 5.1:+51975615 | MS.gene21274:CDS |
| GTCAATGTGCAGTCATTATC+TGG | 0.297729 | 5.1:+51972389 | MS.gene21274:intron |
| TTCTGGTAATTCTTGAATTC+CGG | 0.322524 | 5.1:-51976977 | None:intergenic |
| TCCATTTGACAGGGCATTGA+TGG | 0.336478 | 5.1:+51977849 | MS.gene21274:intron |
| CCTCACAATCACACTATTAC+AGG | 0.355329 | 5.1:+51976776 | MS.gene21274:CDS |
| GCTAAGCAATTTCCCACAAT+TGG | 0.366785 | 5.1:+51978347 | MS.gene21274:CDS |
| AGGCCAAAATTAATAGTTGC+CGG | 0.371671 | 5.1:+51975433 | MS.gene21274:CDS |
| GAAACCTCATGAGAGAATTA+TGG | 0.376002 | 5.1:+51972448 | MS.gene21274:CDS |
| TAGCATAACCTTCTACTTCA+TGG | 0.378616 | 5.1:-51978328 | None:intergenic |
| GAACCCCTGCTCTTACATCT+AGG | 0.381081 | 5.1:+51978059 | MS.gene21274:CDS |
| AAAGGATATGACCTTGTATC+TGG | 0.384516 | 5.1:+51977189 | MS.gene21274:CDS |
| GATGTGTCTGCAATGGTTCC+TGG | 0.395208 | 5.1:+51977936 | MS.gene21274:CDS |
| AGATACAGCTGATATCTTCT+TGG | 0.397878 | 5.1:-51973488 | None:intergenic |
| GAGAGAAGTTGGTTTGTTGA+TGG | 0.399841 | 5.1:-51970158 | None:intergenic |
| TATCCTGGTGCTAGATACTA+TGG | 0.403377 | 5.1:+51970661 | MS.gene21274:CDS |
| CTCTATCTCAAAACCAATTG+TGG | 0.418208 | 5.1:-51978360 | None:intergenic |
| TCGATTATATCTTCGATCTC+AGG | 0.425038 | 5.1:-51970443 | None:intergenic |
| CAAATTAAGGAAAATTCTAA+AGG | 0.428238 | 5.1:+51978148 | MS.gene21274:CDS |
| TGGGTTGGATCCAACACAAT+GGG | 0.433045 | 5.1:+51970844 | MS.gene21274:CDS |
| GTGGGGCTATGATATTCTTC+AGG | 0.434783 | 5.1:+51975737 | MS.gene21274:CDS |
| GCATCTATCACATGGATATC+AGG | 0.439763 | 5.1:+51972493 | MS.gene21274:CDS |
| TCTTTGAAACAATGCCATAC+AGG | 0.439854 | 5.1:+51973515 | MS.gene21274:CDS |
| GACAGGGCATTGATGGTTCA+AGG | 0.442219 | 5.1:+51977856 | MS.gene21274:intron |
| GTGTAAACTTGGAAGTTAGA+AGG | 0.444272 | 5.1:-51972416 | None:intergenic |
| TCAATAAAGCAGTGTAAACT+TGG | 0.458750 | 5.1:-51972427 | None:intergenic |
| CTTAGCAGTTGCATTGAAGC+AGG | 0.467847 | 5.1:+51976799 | MS.gene21274:CDS |
| ACCATTGCAGACACATCTCC+TGG | 0.470380 | 5.1:-51977930 | None:intergenic |
| TATGTACCTCCCCATTGTGT+TGG | 0.472134 | 5.1:-51970854 | None:intergenic |
| AAATTCAGGCACAAAGTTGA+AGG | 0.472831 | 5.1:+51978247 | MS.gene21274:intron |
| CTACGAAGACAAGATAAATC+AGG | 0.475699 | 5.1:+51976727 | MS.gene21274:CDS |
| TGAAAGAGATAAACAAGAAA+GGG | 0.482029 | 5.1:+51975767 | MS.gene21274:CDS |
| TTAAGTTTACCAACACCAAA+TGG | 0.482107 | 5.1:-51977224 | None:intergenic |
| ACAACATCTGCATAATCAAA+AGG | 0.483389 | 5.1:-51975679 | None:intergenic |
| GATCTCCGCCATGAAGTAGA+AGG | 0.487255 | 5.1:+51978320 | MS.gene21274:CDS |
| AACCCCTGCTCTTACATCTA+GGG | 0.487474 | 5.1:+51978060 | MS.gene21274:CDS |
| TGAGGACCACCTTGAAGTCC+AGG | 0.488890 | 5.1:-51976758 | None:intergenic |
| ACCAAGCGATAGAGAGAAGT+TGG | 0.494543 | 5.1:-51970169 | None:intergenic |
| AAATACAGTGAAGGTTATCC+TGG | 0.496861 | 5.1:+51970646 | MS.gene21274:CDS |
| CTGCAGAGCTTGTTAGAGAA+AGG | 0.502927 | 5.1:+51977171 | MS.gene21274:intron |
| GTGCTCTCATCCAACCTGTA+TGG | 0.509528 | 5.1:-51973529 | None:intergenic |
| AGAAGTTGGTTTGTTGATGG+TGG | 0.517830 | 5.1:-51970155 | None:intergenic |
| AGTCACTTCGTGGGCCACGT+GGG | 0.521577 | 5.1:+51975719 | MS.gene21274:CDS |
| CTGAAAGAGATAAACAAGAA+AGG | 0.522116 | 5.1:+51975766 | MS.gene21274:CDS |
| TCTATCTCAAAACCAATTGT+GGG | 0.524634 | 5.1:-51978359 | None:intergenic |
| ATCAGTGGATTAGTTGCTGC+AGG | 0.524776 | 5.1:+51975643 | MS.gene21274:CDS |
| AAACAACTCAACGATCCTCT+TGG | 0.526204 | 5.1:+51970410 | MS.gene21274:CDS |
| ACCAACTTCTCTCTATCGCT+TGG | 0.526527 | 5.1:+51970168 | MS.gene21274:CDS |
| GTTCATGTAGGCTATGACAC+CGG | 0.530615 | 5.1:+51976958 | MS.gene21274:intron |
| TGATGGTTCAAGGGTTGAGA+AGG | 0.532133 | 5.1:+51977866 | MS.gene21274:CDS |
| CAACGATCCTCTTGGTGTAG+TGG | 0.537096 | 5.1:+51970418 | MS.gene21274:CDS |
| TGATTATGCGCGTATTCGCA+AGG | 0.537140 | 5.1:+51975480 | MS.gene21274:CDS |
| TTTGTGTCAGAAGCGTGCAT+TGG | 0.545541 | 5.1:+51970814 | MS.gene21274:CDS |
| AAACCCCTAGATGTAAGAGC+AGG | 0.546239 | 5.1:-51978064 | None:intergenic |
| ATGTGATAGATGCCCACCAT+GGG | 0.548654 | 5.1:-51972483 | None:intergenic |
| GTTCCTGGAGGGATCAGAAT+GGG | 0.551642 | 5.1:+51977951 | MS.gene21274:CDS |
| TCTCAGTTCCACCAGATACA+AGG | 0.554612 | 5.1:-51977200 | None:intergenic |
| CCACCATAGTATCTAGCACC+AGG | 0.554761 | 5.1:-51970664 | None:intergenic |
| GGCTATGATATTCTTCAGGA+AGG | 0.554841 | 5.1:+51975741 | MS.gene21274:CDS |
| GTACCCATTCTGATCCCTCC+AGG | 0.555025 | 5.1:-51977954 | None:intergenic |
| TTGTCGGTGATGCAAGCGGT+TGG | 0.559546 | 5.1:+51970607 | MS.gene21274:CDS |
| TAAACAGAAGGCAGTTATGT+TGG | 0.561749 | 5.1:+51975606 | MS.gene21274:CDS |
| ACAGGGCATTGATGGTTCAA+GGG | 0.562312 | 5.1:+51977857 | MS.gene21274:intron |
| TGAAACAATGCCATACAGGT+TGG | 0.563831 | 5.1:+51973519 | MS.gene21274:CDS |
| AGAGATAAACAAGAAAGGGC+AGG | 0.567037 | 5.1:+51975771 | MS.gene21274:CDS |
| ACCCCTGCTCTTACATCTAG+GGG | 0.567946 | 5.1:+51978061 | MS.gene21274:CDS |
| ACAACACATAAGTCACTTCG+TGG | 0.573831 | 5.1:+51975709 | MS.gene21274:CDS |
| CATGGTGGGCATCTATCACA+TGG | 0.576511 | 5.1:+51972485 | MS.gene21274:CDS |
| AACACAAATCACAATGGCCA+TGG | 0.577099 | 5.1:+51970114 | None:intergenic |
| AAGTCACTTCGTGGGCCACG+TGG | 0.581045 | 5.1:+51975718 | MS.gene21274:CDS |
| TACAGGTTGGATGAGAGCAC+TGG | 0.582002 | 5.1:+51973532 | MS.gene21274:CDS |
| GGTTCCTGGAGGGATCAGAA+TGG | 0.584524 | 5.1:+51977950 | MS.gene21274:CDS |
| GGATATGACCTTGTATCTGG+TGG | 0.585564 | 5.1:+51977192 | MS.gene21274:CDS |
| GGTGATGCAAGCGGTTGGAT+CGG | 0.589257 | 5.1:+51970612 | MS.gene21274:CDS |
| GCTGATATGGCACACATCAG+TGG | 0.589350 | 5.1:+51975628 | MS.gene21274:CDS |
| TGGTGGAACTGAGAACCATT+TGG | 0.589693 | 5.1:+51977209 | MS.gene21274:CDS |
| AACCCCTAGATGTAAGAGCA+GGG | 0.589907 | 5.1:-51978063 | None:intergenic |
| ACGAAGTGACTTATGTGTTG+TGG | 0.593438 | 5.1:-51975707 | None:intergenic |
| AGTGTGTTGAGCGGATAGAG+AGG | 0.594430 | 5.1:-51970280 | None:intergenic |
| TAACTCCTCGACATTTGCAC+AGG | 0.594690 | 5.1:+51977012 | MS.gene21274:CDS |
| TCTGCATAATCAAAAGGAGA+AGG | 0.596550 | 5.1:-51975673 | None:intergenic |
| GTCACTTCGTGGGCCACGTG+GGG | 0.596633 | 5.1:+51975720 | MS.gene21274:CDS |
| TACGCGCATAATCATAAAGA+CGG | 0.597101 | 5.1:-51975471 | None:intergenic |
| TTAGGTCTGTGATAAACAGA+AGG | 0.603056 | 5.1:+51975594 | MS.gene21274:intron |
| GTCGTTGTCGGTGATGCAAG+CGG | 0.608775 | 5.1:+51970603 | MS.gene21274:CDS |
| TTGGGTTGGATCCAACACAA+TGG | 0.608804 | 5.1:+51970843 | MS.gene21274:CDS |
| AACTGAGAACCATTTGGTGT+TGG | 0.610815 | 5.1:+51977215 | MS.gene21274:CDS |
| ACGCGCATAATCATAAAGAC+GGG | 0.612584 | 5.1:-51975470 | None:intergenic |
| TCCAGGAGATGTGTCTGCAA+TGG | 0.614881 | 5.1:+51977929 | MS.gene21274:CDS |
| AGTGCCATAATTCTCTCATG+AGG | 0.623699 | 5.1:-51972452 | None:intergenic |
| ACGGGCATAAGCACTAGCAC+CGG | 0.623924 | 5.1:-51975452 | None:intergenic |
| CATGTGATAGATGCCCACCA+TGG | 0.624147 | 5.1:-51972484 | None:intergenic |
| GTTTACCTGTGCAAATGTCG+AGG | 0.632815 | 5.1:-51977017 | None:intergenic |
| ATGACGAATAAATACAGTGA+AGG | 0.634321 | 5.1:+51970637 | MS.gene21274:CDS |
| GCTATGATATTCTTCAGGAA+GGG | 0.645121 | 5.1:+51975742 | MS.gene21274:CDS |
| AGTGAGTCTGCGAAGTGCCA+TGG | 0.645311 | 5.1:-51970131 | None:intergenic |
| ACCCCTAGATGTAAGAGCAG+GGG | 0.651380 | 5.1:-51978062 | None:intergenic |
| TTTGATAAGGTACATTGACA+TGG | 0.655626 | 5.1:+51970784 | MS.gene21274:intron |
| GGGTTGGATCCAACACAATG+GGG | 0.659140 | 5.1:+51970845 | MS.gene21274:CDS |
| TGTCTGCAATGGTTCCTGGA+GGG | 0.662301 | 5.1:+51977940 | MS.gene21274:CDS |
| TGGTTATATTGACTATGACC+AGG | 0.663546 | 5.1:+51973552 | MS.gene21274:CDS |
| AGATTTGTGAGTGTGTTGAG+CGG | 0.663584 | 5.1:-51970289 | None:intergenic |
| GTTGGTAAACTTAAGAAACA+AGG | 0.669487 | 5.1:+51977233 | MS.gene21274:CDS |
| CCTGGTGCTAGATACTATGG+TGG | 0.671775 | 5.1:+51970664 | MS.gene21274:CDS |
| TCAGGATCCACTACACCAAG+AGG | 0.682850 | 5.1:-51970425 | None:intergenic |
| CAACACATAAGTCACTTCGT+GGG | 0.684698 | 5.1:+51975710 | MS.gene21274:CDS |
| TTGGATCCAACACAATGGGG+AGG | 0.686303 | 5.1:+51970848 | MS.gene21274:CDS |
| GAGGACCACCTTGAAGTCCA+GGG | 0.687951 | 5.1:-51976757 | None:intergenic |
| CCTGTAATAGTGTGATTGTG+AGG | 0.688267 | 5.1:-51976776 | None:intergenic |
| TGTGATAGATGCCCACCATG+GGG | 0.693725 | 5.1:-51972482 | None:intergenic |
| AAGAATATCATAGCCCCACG+TGG | 0.696480 | 5.1:-51975733 | None:intergenic |
| GTGATAGATGCCCACCATGG+GGG | 0.708056 | 5.1:-51972481 | None:intergenic |
| AGTAGCTGAATACTTTGACG+CGG | 0.710734 | 5.1:+51978108 | MS.gene21274:CDS |
| GTGTCTGCAATGGTTCCTGG+AGG | 0.723786 | 5.1:+51977939 | MS.gene21274:CDS |
| CATAACCTTCTACTTCATGG+CGG | 0.732603 | 5.1:-51978325 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 51970127 | 51978403 | 51970127 | ID=MS.gene21274 |
| chr5.1 | mRNA | 51970127 | 51978403 | 51970127 | ID=MS.gene21274.t1;Parent=MS.gene21274 |
| chr5.1 | exon | 51970127 | 51970189 | 51970127 | ID=MS.gene21274.t1.exon1;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51970127 | 51970189 | 51970127 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51970276 | 51970320 | 51970276 | ID=MS.gene21274.t1.exon2;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51970276 | 51970320 | 51970276 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51970404 | 51970490 | 51970404 | ID=MS.gene21274.t1.exon3;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51970404 | 51970490 | 51970404 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51970571 | 51970692 | 51970571 | ID=MS.gene21274.t1.exon4;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51970571 | 51970692 | 51970571 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51970793 | 51970869 | 51970793 | ID=MS.gene21274.t1.exon5;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51970793 | 51970869 | 51970793 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51972390 | 51972514 | 51972390 | ID=MS.gene21274.t1.exon6;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51972390 | 51972514 | 51972390 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51973481 | 51973573 | 51973481 | ID=MS.gene21274.t1.exon7;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51973481 | 51973573 | 51973481 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51975409 | 51975501 | 51975409 | ID=MS.gene21274.t1.exon8;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51975409 | 51975501 | 51975409 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51975598 | 51975795 | 51975598 | ID=MS.gene21274.t1.exon9;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51975598 | 51975795 | 51975598 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51976716 | 51976820 | 51976716 | ID=MS.gene21274.t1.exon10;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51976716 | 51976820 | 51976716 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51976968 | 51977033 | 51976968 | ID=MS.gene21274.t1.exon11;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51976968 | 51977033 | 51976968 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51977177 | 51977254 | 51977177 | ID=MS.gene21274.t1.exon12;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51977177 | 51977254 | 51977177 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51977861 | 51977972 | 51977861 | ID=MS.gene21274.t1.exon13;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51977861 | 51977972 | 51977861 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51978059 | 51978169 | 51978059 | ID=MS.gene21274.t1.exon14;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51978059 | 51978169 | 51978059 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| chr5.1 | exon | 51978255 | 51978403 | 51978255 | ID=MS.gene21274.t1.exon15;Parent=MS.gene21274.t1 |
| chr5.1 | CDS | 51978255 | 51978403 | 51978255 | ID=cds.MS.gene21274.t1;Parent=MS.gene21274.t1 |
| Gene Sequence |
| Protein sequence |
