AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066957


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066957.t1 MTR_6g471080 90.521 211 19 1 1 210 1 211 5.90e-126 354
MS.gene066957.t1 MTR_6g471090 80.488 205 39 1 3 206 6 210 3.58e-115 327
MS.gene066957.t1 MTR_6g071410 75.943 212 45 2 3 208 7 218 2.28e-107 307
MS.gene066957.t1 MTR_6g070890 75.943 212 45 2 3 208 7 218 2.28e-107 307
MS.gene066957.t1 MTR_6g070900 72.596 208 55 2 3 208 7 214 4.37e-99 286
MS.gene066957.t1 MTR_6g071400 72.596 208 55 2 3 208 7 214 4.37e-99 286
MS.gene066957.t1 MTR_6g071710 72.368 152 41 1 58 208 11 162 5.49e-69 208
MS.gene066957.t1 MTR_2g048270 59.091 154 55 5 45 197 2 148 9.51e-57 177
MS.gene066957.t1 MTR_1g057270 43.827 162 81 7 24 182 74 228 5.06e-32 116
MS.gene066957.t1 MTR_4g119428 46.212 132 68 3 17 146 65 195 4.49e-31 114
MS.gene066957.t1 MTR_7g106030 34.483 203 112 8 6 194 33 228 1.64e-30 112
MS.gene066957.t1 MTR_7g106010 36.170 188 110 6 11 194 43 224 2.29e-30 112
MS.gene066957.t1 MTR_7g105870 43.871 155 80 5 22 174 58 207 1.19e-25 99.8
MS.gene066957.t1 MTR_7g105970 31.553 206 112 10 5 194 31 223 1.34e-24 97.1
MS.gene066957.t1 MTR_7g106000 33.505 194 106 9 5 183 31 216 1.51e-24 96.7
MS.gene066957.t1 MTR_1g057400 31.606 193 104 9 5 178 10 193 3.77e-21 87.4
MS.gene066957.t1 MTR_6g453010 36.047 172 84 5 11 176 17 168 7.49e-20 83.2
MS.gene066957.t1 MTR_2g011350 34.884 86 55 1 20 104 37 122 1.95e-14 69.3
MS.gene066957.t1 MTR_7g106050 28.931 159 105 5 6 161 19 172 2.32e-14 69.3
MS.gene066957.t1 MTR_3g063310 34.815 135 75 5 21 148 83 211 8.91e-14 68.6
MS.gene066957.t1 MTR_1g057290 34.503 171 97 8 30 193 51 213 1.47e-12 64.7
MS.gene066957.t1 MTR_2g086790 26.316 171 109 5 4 161 16 182 2.14e-12 64.3
MS.gene066957.t1 MTR_1g019920 42.105 76 38 3 19 89 3 77 4.56e-12 60.1
MS.gene066957.t1 MTR_2g087000 26.136 176 117 4 9 180 52 218 5.04e-12 63.2
MS.gene066957.t1 MTR_5g076170 33.071 127 82 3 24 148 97 222 7.33e-12 63.2
MS.gene066957.t1 MTR_1g057420 32.609 138 87 5 26 161 39 172 2.21e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066957.t1 AT2G35980 44.318 176 81 6 12 174 35 206 1.26e-36 128
MS.gene066957.t1 AT5G06320 39.247 186 102 8 11 194 52 228 2.24e-31 114
MS.gene066957.t1 AT2G35460 35.263 190 113 7 7 194 54 235 9.43e-31 113
MS.gene066957.t1 AT3G11650 32.812 192 121 6 4 194 52 236 3.01e-29 109
MS.gene066957.t1 AT3G52470 34.545 110 70 2 4 111 16 125 2.50e-16 74.7
MS.gene066957.t1 AT1G32270 30.968 155 100 5 28 180 1 150 6.84e-16 75.5
MS.gene066957.t1 AT1G32270 30.968 155 100 5 28 180 1 150 1.58e-15 74.7
MS.gene066957.t1 AT3G11660 29.940 167 99 6 4 161 15 172 1.33e-14 70.1
MS.gene066957.t1 AT4G09590 29.139 151 102 5 6 154 21 168 6.06e-14 68.2
MS.gene066957.t1 AT2G35960 30.769 143 93 5 21 161 35 173 1.31e-12 64.7
MS.gene066957.t1 AT2G35970 29.139 151 102 5 6 154 21 168 1.46e-12 64.3
MS.gene066957.t1 AT5G22870 25.532 188 118 6 10 184 24 202 1.65e-12 64.3
MS.gene066957.t1 AT1G65690 29.448 163 100 6 9 157 71 232 9.62e-12 62.8
MS.gene066957.t1 AT3G44220 30.612 147 94 6 16 160 31 171 1.12e-11 62.0

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTAGGACTAAGAGAAAA+TGG 0.290514 6.4:-48014659 None:intergenic
TCTTGGGATTTATAAAGATT+TGG 0.312711 6.4:+48014502 MS.gene066957:CDS
TAATCACCATCATCGTCTTC+TGG 0.338455 6.4:+48014162 MS.gene066957:CDS
GAACCACTGTTACATCTTCT+AGG 0.339363 6.4:-48014674 None:intergenic
TTTATAAAAGCTTCCATACT+TGG 0.377157 6.4:-48014552 None:intergenic
TCGGCGTTTGAGAGTTCCTT+TGG 0.409123 6.4:+48014607 MS.gene066957:CDS
GTTCGAATTAGGGCCAAGTA+TGG 0.417987 6.4:+48014539 MS.gene066957:CDS
AAACGCCGACACTGGACAAC+CGG 0.453051 6.4:-48014593 None:intergenic
ACGTGAAATTTCACACTTGA+GGG 0.456152 6.4:-48014197 None:intergenic
ATAGACTATGATGTTGTTGT+TGG 0.461075 6.4:-48014303 None:intergenic
TAGACTATGATGTTGTTGTT+GGG 0.464761 6.4:-48014302 None:intergenic
CTGCTGCTTTATTTGCATCA+CGG 0.469747 6.4:-48014709 None:intergenic
ACTTTGACACCCTTTGATCA+AGG 0.484433 6.4:+48014380 MS.gene066957:CDS
TGGTGATGATGAGTTACTAT+TGG 0.487019 6.4:-48014639 None:intergenic
TATTCTGAAGAGACACGTCT+TGG 0.496905 6.4:+48014485 MS.gene066957:CDS
GGAAATTCTATCATCAAACT+TGG 0.500531 6.4:+48014443 MS.gene066957:CDS
CTTGGACCTATCGAATTCAA+AGG 0.500883 6.4:+48014422 MS.gene066957:CDS
AAGTGGTCGGTTTAATCCAC+CGG 0.502974 6.4:+48014574 MS.gene066957:CDS
GAACTCTCAAACGCCGACAC+TGG 0.505962 6.4:-48014601 None:intergenic
ACCTCACCACAAATATGGGT+TGG 0.517407 6.4:+48014093 None:intergenic
CCTCACCACAAATATGGGTT+GGG 0.518502 6.4:+48014094 None:intergenic
ATTCTGAAGAGACACGTCTT+GGG 0.539671 6.4:+48014486 MS.gene066957:CDS
AGTCTATTATCGAAGAATCA+AGG 0.541807 6.4:+48014319 MS.gene066957:CDS
ATCCACCGGTTGTCCAGTGT+CGG 0.543668 6.4:+48014588 MS.gene066957:CDS
AGACTATGATGTTGTTGTTG+GGG 0.552465 6.4:-48014301 None:intergenic
TTCGATAGGTCCAAGAAAAG+TGG 0.557190 6.4:-48014414 None:intergenic
GAAGAATCAAGGCCATTGCT+TGG 0.570010 6.4:+48014330 MS.gene066957:CDS
AATATAAGTTGTTGTTTGTG+AGG 0.572559 6.4:-48014247 None:intergenic
TACGTGAAATTTCACACTTG+AGG 0.573509 6.4:-48014198 None:intergenic
GAATTTCCTTTGAATTCGAT+AGG 0.573832 6.4:-48014428 None:intergenic
CGCCGACACTGGACAACCGG+TGG 0.576977 6.4:-48014590 None:intergenic
ATTGTCTTTGTACCAAGCAA+TGG 0.580644 6.4:-48014342 None:intergenic
TATTATCCAGAAGACGATGA+TGG 0.595315 6.4:-48014168 None:intergenic
GTTGTTTGTGAGGTTGAACT+CGG 0.599773 6.4:-48014237 None:intergenic
GAACTCGGTGAGAGTTGCAT+CGG 0.601986 6.4:-48014222 None:intergenic
CGATGATGGTGATTACGAAG+AGG 0.658850 6.4:-48014154 None:intergenic
AGTCCTAGAAGATGTAACAG+TGG 0.698011 6.4:+48014671 MS.gene066957:CDS
CTATTGGAAGAAGAAACCAA+AGG 0.773456 6.4:-48014623 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCTTGGGATTTATAAAGATT+TGG + chr6.4:48014502-48014521 MS.gene066957:CDS 25.0%
! TTTATAAAAGCTTCCATACT+TGG - chr6.4:48014555-48014574 None:intergenic 25.0%
!! TATGGAAGCTTTTATAAAAG+TGG + chr6.4:48014557-48014576 MS.gene066957:CDS 25.0%
!!! AATATAAGTTGTTGTTTGTG+AGG - chr6.4:48014250-48014269 None:intergenic 25.0%
!!! TGATTTTGATGTTCGAATTA+GGG + chr6.4:48014529-48014548 MS.gene066957:CDS 25.0%
!!! TTGATTTTGATGTTCGAATT+AGG + chr6.4:48014528-48014547 MS.gene066957:CDS 25.0%
AGTCTATTATCGAAGAATCA+AGG + chr6.4:48014319-48014338 MS.gene066957:CDS 30.0%
ATAGACTATGATGTTGTTGT+TGG - chr6.4:48014306-48014325 None:intergenic 30.0%
GAATTTCCTTTGAATTCGAT+AGG - chr6.4:48014431-48014450 None:intergenic 30.0%
GGAAATTCTATCATCAAACT+TGG + chr6.4:48014443-48014462 MS.gene066957:CDS 30.0%
! ATTTTTGTGTCCTTGATCAA+AGG - chr6.4:48014393-48014412 None:intergenic 30.0%
! GAAGCTTTTATAAAAGTGGT+CGG + chr6.4:48014561-48014580 MS.gene066957:CDS 30.0%
! TAGACTATGATGTTGTTGTT+GGG - chr6.4:48014305-48014324 None:intergenic 30.0%
! TTTTTGTGTCCTTGATCAAA+GGG - chr6.4:48014392-48014411 None:intergenic 30.0%
!! CACAAAAATACCACTTTTCT+TGG + chr6.4:48014404-48014423 MS.gene066957:CDS 30.0%
ACGTGAAATTTCACACTTGA+GGG - chr6.4:48014200-48014219 None:intergenic 35.0%
ATTGTCTTTGTACCAAGCAA+TGG - chr6.4:48014345-48014364 None:intergenic 35.0%
CTATTGGAAGAAGAAACCAA+AGG - chr6.4:48014626-48014645 None:intergenic 35.0%
TACGTGAAATTTCACACTTG+AGG - chr6.4:48014201-48014220 None:intergenic 35.0%
TATTATCCAGAAGACGATGA+TGG - chr6.4:48014171-48014190 None:intergenic 35.0%
! AGACTATGATGTTGTTGTTG+GGG - chr6.4:48014304-48014323 None:intergenic 35.0%
! TGGTGATGATGAGTTACTAT+TGG - chr6.4:48014642-48014661 None:intergenic 35.0%
!! CTTCTAGGACTAAGAGAAAA+TGG - chr6.4:48014662-48014681 None:intergenic 35.0%
ACTTTGACACCCTTTGATCA+AGG + chr6.4:48014380-48014399 MS.gene066957:CDS 40.0%
AGTCCTAGAAGATGTAACAG+TGG + chr6.4:48014671-48014690 MS.gene066957:CDS 40.0%
CTTGGACCTATCGAATTCAA+AGG + chr6.4:48014422-48014441 MS.gene066957:CDS 40.0%
GAACCACTGTTACATCTTCT+AGG - chr6.4:48014677-48014696 None:intergenic 40.0%
GTTGTTTGTGAGGTTGAACT+CGG - chr6.4:48014240-48014259 None:intergenic 40.0%
TAATCACCATCATCGTCTTC+TGG + chr6.4:48014162-48014181 MS.gene066957:CDS 40.0%
TTCGATAGGTCCAAGAAAAG+TGG - chr6.4:48014417-48014436 None:intergenic 40.0%
! ATTCTGAAGAGACACGTCTT+GGG + chr6.4:48014486-48014505 MS.gene066957:CDS 40.0%
! TATTCTGAAGAGACACGTCT+TGG + chr6.4:48014485-48014504 MS.gene066957:CDS 40.0%
!! CTGCTGCTTTATTTGCATCA+CGG - chr6.4:48014712-48014731 None:intergenic 40.0%
GAAGAATCAAGGCCATTGCT+TGG + chr6.4:48014330-48014349 MS.gene066957:CDS 45.0%
GTTCGAATTAGGGCCAAGTA+TGG + chr6.4:48014539-48014558 MS.gene066957:CDS 45.0%
! AAGTGGTCGGTTTAATCCAC+CGG + chr6.4:48014574-48014593 MS.gene066957:CDS 45.0%
! CGATGATGGTGATTACGAAG+AGG - chr6.4:48014157-48014176 None:intergenic 45.0%
TCGGCGTTTGAGAGTTCCTT+TGG + chr6.4:48014607-48014626 MS.gene066957:CDS 50.0%
! GAACTCGGTGAGAGTTGCAT+CGG - chr6.4:48014225-48014244 None:intergenic 50.0%
AAACGCCGACACTGGACAAC+CGG - chr6.4:48014596-48014615 None:intergenic 55.0%
GAACTCTCAAACGCCGACAC+TGG - chr6.4:48014604-48014623 None:intergenic 55.0%
! ATCCACCGGTTGTCCAGTGT+CGG + chr6.4:48014588-48014607 MS.gene066957:CDS 55.0%
CGCCGACACTGGACAACCGG+TGG - chr6.4:48014593-48014612 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 48014107 48014739 48014107 ID=MS.gene066957
chr6.4 mRNA 48014107 48014739 48014107 ID=MS.gene066957.t1;Parent=MS.gene066957
chr6.4 exon 48014107 48014739 48014107 ID=MS.gene066957.t1.exon1;Parent=MS.gene066957.t1
chr6.4 CDS 48014107 48014739 48014107 ID=cds.MS.gene066957.t1;Parent=MS.gene066957.t1
Gene Sequence

>MS.gene066957

ATGGGTTGGGTCGCACGCATTTGCTGCACATTATGTTGTCTTATAGTCCTCTTCGTAATCACCATCATCGTCTTCTGGATAATAGTCTCTCCCTCAAGTGTGAAATTTCACGTAACCGATGCAACTCTCACCGAGTTCAACCTCACAAACAACAACTTATATTACAATTTCAAAGTGAATGTCACAGCAAGAAACCCCAACAACAACATCATAGTCTATTATCGAAGAATCAAGGCCATTGCTTGGTACAAAGACAATGATTTCAGTCATATAACTTTGACACCCTTTGATCAAGGACACAAAAATACCACTTTTCTTGGACCTATCGAATTCAAAGGAAATTCTATCATCAAACTTGGACGTCAACAACTTAACGAGTATTCTGAAGAGACACGTCTTGGGATTTATAAAGATTTGGCTGTTGATTTTGATGTTCGAATTAGGGCCAAGTATGGAAGCTTTTATAAAAGTGGTCGGTTTAATCCACCGGTTGTCCAGTGTCGGCGTTTGAGAGTTCCTTTGGTTTCTTCTTCCAATAGTAACTCATCATCACCATTTTCTCTTAGTCCTAGAAGATGTAACAGTGGTTCTTTCTTTACAGACCGTGATGCAAATAAAGCAGCAGCAGCTTGA

Protein sequence

>MS.gene066957.t1

MGWVARICCTLCCLIVLFVITIIVFWIIVSPSSVKFHVTDATLTEFNLTNNNLYYNFKVNVTARNPNNNIIVYYRRIKAIAWYKDNDFSHITLTPFDQGHKNTTFLGPIEFKGNSIIKLGRQQLNEYSEETRLGIYKDLAVDFDVRIRAKYGSFYKSGRFNPPVVQCRRLRVPLVSSSNSNSSSPFSLSPRRCNSGSFFTDRDANKAAAA