AlfalfaGEDB | MS.gene06751

M. sativa cultivar XinJiangDaYe / MS.gene06751

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06751.t1	KEH35442.1	97	166	5	0	4	169	1	166	2.80E-79	304.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06751.t1	Q9ZNX9	48.2	112	55	1	4	112	1	112	4.9e-16	85.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06751.t1	A0A072V271	97.0	166	5	0	4	169	1	166	2.0e-79	304.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene06751	0.84444	7.55E-59	-1.69E-46
MS.gene049356	MS.gene06751	0.851701	7.48E-61	-1.69E-46
MS.gene049369	MS.gene06751	0.875482	2.98E-68	-1.69E-46
MS.gene049396	MS.gene06751	0.910494	1.76E-82	-1.69E-46
MS.gene049609	MS.gene06751	0.800806	1.21E-48	-1.69E-46
MS.gene049907	MS.gene06751	0.803792	2.94E-49	-1.69E-46
MS.gene050122	MS.gene06751	0.800872	1.18E-48	-1.69E-46
MS.gene050170	MS.gene06751	0.819876	9.22E-53	-1.69E-46
MS.gene050216	MS.gene06751	0.812384	4.36E-51	-1.69E-46
MS.gene050323	MS.gene06751	0.858591	7.42E-63	-1.69E-46
MS.gene050496	MS.gene06751	0.808998	2.35E-50	-1.69E-46
MS.gene050499	MS.gene06751	0.84601	2.84E-59	-1.69E-46
MS.gene050501	MS.gene06751	0.807449	5.03E-50	-1.69E-46
MS.gene050502	MS.gene06751	0.902152	1.30E-78	-1.69E-46
MS.gene050520	MS.gene06751	0.833533	5.05E-56	-1.69E-46
MS.gene050662	MS.gene06751	0.803876	2.83E-49	-1.69E-46
MS.gene050686	MS.gene06751	0.810254	1.26E-50	-1.69E-46
MS.gene050697	MS.gene06751	0.847629	1.02E-59	-1.69E-46
MS.gene050772	MS.gene06751	0.821718	3.48E-53	-1.69E-46
MS.gene05080	MS.gene06751	0.813426	2.58E-51	-1.69E-46
MS.gene050868	MS.gene06751	0.839196	1.83E-57	-1.69E-46
MS.gene050871	MS.gene06751	0.842071	3.23E-58	-1.69E-46
MS.gene051268	MS.gene06751	0.816685	4.87E-52	-1.69E-46
MS.gene051509	MS.gene06751	0.893737	4.75E-75	-1.69E-46
MS.gene051734	MS.gene06751	0.833169	6.22E-56	-1.69E-46
MS.gene051838	MS.gene06751	0.800568	1.36E-48	-1.69E-46
MS.gene051874	MS.gene06751	0.834289	3.27E-56	-1.69E-46
MS.gene051941	MS.gene06751	0.845275	4.50E-59	-1.69E-46
MS.gene052066	MS.gene06751	0.823042	1.71E-53	-1.69E-46
MS.gene052164	MS.gene06751	0.817083	3.96E-52	-1.69E-46
MS.gene052346	MS.gene06751	0.825301	5.05E-54	-1.69E-46
MS.gene052414	MS.gene06751	0.856458	3.17E-62	-1.69E-46
MS.gene052822	MS.gene06751	0.802468	5.53E-49	-1.69E-46
MS.gene052966	MS.gene06751	0.82564	4.20E-54	-1.69E-46
MS.gene052999	MS.gene06751	0.815352	9.66E-52	-1.69E-46
MS.gene053018	MS.gene06751	0.80971	1.65E-50	-1.69E-46
MS.gene053449	MS.gene06751	0.875421	3.12E-68	-1.69E-46
MS.gene053505	MS.gene06751	0.87494	4.56E-68	-1.69E-46
MS.gene053574	MS.gene06751	0.844235	8.57E-59	-1.69E-46
MS.gene053626	MS.gene06751	0.832101	1.15E-55	-1.69E-46
MS.gene053633	MS.gene06751	0.802725	4.90E-49	-1.69E-46
MS.gene053813	MS.gene06751	0.80886	2.52E-50	-1.69E-46
MS.gene053824	MS.gene06751	0.825929	3.58E-54	-1.69E-46
MS.gene053889	MS.gene06751	0.834119	3.60E-56	-1.69E-46
MS.gene053912	MS.gene06751	0.847352	1.22E-59	-1.69E-46
MS.gene053916	MS.gene06751	0.831027	2.11E-55	-1.69E-46
MS.gene054595	MS.gene06751	0.823607	1.26E-53	-1.69E-46
MS.gene05494	MS.gene06751	0.863526	2.34E-64	-1.69E-46
MS.gene05505	MS.gene06751	0.809776	1.60E-50	-1.69E-46
MS.gene055224	MS.gene06751	0.843581	1.28E-58	-1.69E-46
MS.gene055722	MS.gene06751	0.819664	1.03E-52	-1.69E-46
MS.gene055741	MS.gene06751	0.847694	9.83E-60	-1.69E-46
MS.gene055744	MS.gene06751	0.84504	5.20E-59	-1.69E-46
MS.gene055750	MS.gene06751	0.817807	2.72E-52	-1.69E-46
MS.gene05589	MS.gene06751	0.816047	6.76E-52	-1.69E-46
MS.gene055909	MS.gene06751	0.861239	1.18E-63	-1.69E-46
MS.gene055911	MS.gene06751	0.857491	1.57E-62	-1.69E-46
MS.gene056166	MS.gene06751	0.852068	5.88E-61	-1.69E-46
MS.gene056387	MS.gene06751	0.841741	3.95E-58	-1.69E-46
MS.gene056422	MS.gene06751	0.895542	8.68E-76	-1.69E-46
MS.gene056446	MS.gene06751	0.808698	2.73E-50	-1.69E-46
MS.gene056487	MS.gene06751	0.800004	1.77E-48	-1.69E-46
MS.gene056504	MS.gene06751	0.835554	1.57E-56	-1.69E-46
MS.gene056511	MS.gene06751	0.827639	1.40E-54	-1.69E-46
MS.gene05664	MS.gene06751	0.807293	5.43E-50	-1.69E-46
MS.gene05666	MS.gene06751	0.821394	4.13E-53	-1.69E-46
MS.gene056878	MS.gene06751	0.809333	1.99E-50	-1.69E-46
MS.gene057019	MS.gene06751	0.847842	8.95E-60	-1.69E-46
MS.gene057076	MS.gene06751	0.828264	9.90E-55	-1.69E-46
MS.gene057086	MS.gene06751	0.890208	1.21E-73	-1.69E-46
MS.gene057331	MS.gene06751	0.800376	1.48E-48	-1.69E-46
MS.gene057537	MS.gene06751	0.821365	4.20E-53	-1.69E-46
MS.gene057734	MS.gene06751	0.855796	4.96E-62	-1.69E-46
MS.gene05787	MS.gene06751	0.830691	2.55E-55	-1.69E-46
MS.gene057926	MS.gene06751	0.810476	1.13E-50	-1.69E-46
MS.gene058075	MS.gene06751	0.829273	5.64E-55	-1.69E-46
MS.gene058120	MS.gene06751	0.857398	1.68E-62	-1.69E-46
MS.gene058459	MS.gene06751	0.866912	2.02E-65	-1.69E-46
MS.gene058948	MS.gene06751	0.828434	9.01E-55	-1.69E-46
MS.gene058978	MS.gene06751	-0.802824	4.67E-49	-1.69E-46
MS.gene059037	MS.gene06751	-0.806075	9.81E-50	-1.69E-46
MS.gene059129	MS.gene06751	0.839331	1.69E-57	-1.69E-46
MS.gene05913	MS.gene06751	0.842442	2.58E-58	-1.69E-46
MS.gene059305	MS.gene06751	0.85754	1.52E-62	-1.69E-46
MS.gene059367	MS.gene06751	0.874399	6.96E-68	-1.69E-46
MS.gene059368	MS.gene06751	0.861719	8.42E-64	-1.69E-46
MS.gene059517	MS.gene06751	0.837784	4.24E-57	-1.69E-46
MS.gene059667	MS.gene06751	0.834633	2.68E-56	-1.69E-46
MS.gene059674	MS.gene06751	0.863481	2.41E-64	-1.69E-46
MS.gene059680	MS.gene06751	0.867333	1.48E-65	-1.69E-46
MS.gene059711	MS.gene06751	0.838317	3.09E-57	-1.69E-46
MS.gene059807	MS.gene06751	0.857619	1.44E-62	-1.69E-46
MS.gene059865	MS.gene06751	0.838407	2.93E-57	-1.69E-46
MS.gene06000	MS.gene06751	0.823131	1.63E-53	-1.69E-46
MS.gene060372	MS.gene06751	0.889416	2.47E-73	-1.69E-46
MS.gene060401	MS.gene06751	0.805454	1.32E-49	-1.69E-46
MS.gene060482	MS.gene06751	0.836148	1.11E-56	-1.69E-46
MS.gene060778	MS.gene06751	0.856326	3.47E-62	-1.69E-46
MS.gene060835	MS.gene06751	0.841517	4.53E-58	-1.69E-46
MS.gene060837	MS.gene06751	0.854782	9.79E-62	-1.69E-46
MS.gene06097	MS.gene06751	0.904906	7.52E-80	-1.69E-46
MS.gene060982	MS.gene06751	0.833023	6.77E-56	-1.69E-46
MS.gene061218	MS.gene06751	0.82926	5.69E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGTTAACGGTAGCATTTAA+AGG	0.276378	3.2:+76725037	MS.gene06751:CDS
AAGAGGATTTAAACAGTGAT+AGG	0.297201	3.2:+76725359	MS.gene06751:CDS
CCTTTAAATGCTACCGTTAA+CGG	0.304115	3.2:-76725037	None:intergenic
GAGACAGCTCTTGGTTTCTT+AGG	0.309175	3.2:-76725166	None:intergenic
CTCATCAAGATTCATCCGTT+TGG	0.343384	3.2:-76725407	None:intergenic
TCCTCAGTGGAGACAGCTCT+TGG	0.384406	3.2:-76725175	None:intergenic
ATAATCTGCATCACATATAT+CGG	0.410715	3.2:-76725278	None:intergenic
CAGCCAAGACGCACAAGAAA+AGG	0.419331	3.2:+76725122	MS.gene06751:CDS
CTGATTAGGCAAACTTGGTA+TGG	0.439102	3.2:+76724928	None:intergenic
AAACACTGATTAGGCAAACT+TGG	0.465435	3.2:+76724923	None:intergenic
CATCACATATATCGGTTGTC+GGG	0.482870	3.2:-76725270	None:intergenic
TGTATTGAAGAGACCGTTAA+CGG	0.487228	3.2:+76725024	MS.gene06751:CDS
GCATCACATATATCGGTTGT+CGG	0.491957	3.2:-76725271	None:intergenic
TAATCCACATCCAATGAAGA+TGG	0.497477	3.2:-76725205	None:intergenic
TCTCGAAGGGGTAAGAAGAT+TGG	0.504636	3.2:+76725325	MS.gene06751:CDS
GTTAACGGTCTCTTCAATAC+AGG	0.512611	3.2:-76725022	None:intergenic
GATTGGTGATGTAAGTAAAG+AGG	0.522341	3.2:+76725342	MS.gene06751:CDS
CTGTTTCAAGCTCAGCTGCT+CGG	0.525455	3.2:+76724960	MS.gene06751:CDS
ACCAGAAAACAAAAGCCAAA+CGG	0.546934	3.2:+76725392	MS.gene06751:CDS
AGAGGATTTAAACAGTGATA+GGG	0.568935	3.2:+76725360	MS.gene06751:CDS
TCAGCTGCTCGGACTCCACT+AGG	0.575229	3.2:+76724971	MS.gene06751:CDS
GATCAAAAGAGTCTCTCGAA+GGG	0.598531	3.2:+76725312	MS.gene06751:CDS
AACTTTGTATTCAATCCTAG+TGG	0.599283	3.2:-76724986	None:intergenic
TGATTAGGCAAACTTGGTAT+GGG	0.604520	3.2:+76724929	None:intergenic
AACGGATGAATCTTGATGAG+AGG	0.617893	3.2:+76725410	MS.gene06751:CDS
AGATCAAAAGAGTCTCTCGA+AGG	0.624213	3.2:+76725311	MS.gene06751:CDS
TAAACAGAATGACACAGCGT+TGG	0.627600	3.2:+76725063	MS.gene06751:CDS
GGATTTAAACAGTGATAGGG+TGG	0.634412	3.2:+76725363	MS.gene06751:CDS
ATCAAAAGAGTCTCTCGAAG+GGG	0.655049	3.2:+76725313	MS.gene06751:CDS
ACGGTCTCTTCAATACAGGA+TGG	0.670415	3.2:-76725018	None:intergenic
ACCAAGAGCTGTCTCCACTG+AGG	0.712371	3.2:+76725174	MS.gene06751:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGTATATATTTTCAAGTA+TGG	-	chr3.2:76725245-76725264	None:intergenic	15.0%
!	ATAATCTGCATCACATATAT+CGG	-	chr3.2:76725281-76725300	None:intergenic	25.0%
	AACTTTGTATTCAATCCTAG+TGG	-	chr3.2:76724989-76725008	None:intergenic	30.0%
	AAGAGGATTTAAACAGTGAT+AGG	+	chr3.2:76725359-76725378	MS.gene06751:CDS	30.0%
	AGAGGATTTAAACAGTGATA+GGG	+	chr3.2:76725360-76725379	MS.gene06751:CDS	30.0%
!	GGAAAATTTTCCATCTTCAT+TGG	+	chr3.2:76725195-76725214	MS.gene06751:CDS	30.0%
	ACCAGAAAACAAAAGCCAAA+CGG	+	chr3.2:76725392-76725411	MS.gene06751:CDS	35.0%
	CCTTTAAATGCTACCGTTAA+CGG	-	chr3.2:76725040-76725059	None:intergenic	35.0%
	GTTAAAAAACGAGCGACAAT+AGG	+	chr3.2:76725438-76725457	MS.gene06751:CDS	35.0%
	TAATCCACATCCAATGAAGA+TGG	-	chr3.2:76725208-76725227	None:intergenic	35.0%
	TGTATTGAAGAGACCGTTAA+CGG	+	chr3.2:76725024-76725043	MS.gene06751:CDS	35.0%
!	AGATGGAAAATTTTCCTCAG+TGG	-	chr3.2:76725191-76725210	None:intergenic	35.0%
!	TTTTCCATCTTCATTGGATG+TGG	+	chr3.2:76725201-76725220	MS.gene06751:CDS	35.0%
!!	GATTGGTGATGTAAGTAAAG+AGG	+	chr3.2:76725342-76725361	MS.gene06751:CDS	35.0%
!!!	TGGCTTTTGTTTTCTGGTTT+CGG	-	chr3.2:76725390-76725409	None:intergenic	35.0%
	AACGGATGAATCTTGATGAG+AGG	+	chr3.2:76725410-76725429	MS.gene06751:CDS	40.0%
	AGATCAAAAGAGTCTCTCGA+AGG	+	chr3.2:76725311-76725330	MS.gene06751:CDS	40.0%
	ATCAAAAGAGTCTCTCGAAG+GGG	+	chr3.2:76725313-76725332	MS.gene06751:CDS	40.0%
	CCGTTAACGGTAGCATTTAA+AGG	+	chr3.2:76725037-76725056	MS.gene06751:CDS	40.0%
	CTCATCAAGATTCATCCGTT+TGG	-	chr3.2:76725410-76725429	None:intergenic	40.0%
	GATCAAAAGAGTCTCTCGAA+GGG	+	chr3.2:76725312-76725331	MS.gene06751:CDS	40.0%
	GGATTTAAACAGTGATAGGG+TGG	+	chr3.2:76725363-76725382	MS.gene06751:CDS	40.0%
	GTTAACGGTCTCTTCAATAC+AGG	-	chr3.2:76725025-76725044	None:intergenic	40.0%
	TAAACAGAATGACACAGCGT+TGG	+	chr3.2:76725063-76725082	MS.gene06751:CDS	40.0%
!	CATCACATATATCGGTTGTC+GGG	-	chr3.2:76725273-76725292	None:intergenic	40.0%
!	GCATCACATATATCGGTTGT+CGG	-	chr3.2:76725274-76725293	None:intergenic	40.0%
!	TATCCTTTTCTTGTGCGTCT+TGG	-	chr3.2:76725128-76725147	None:intergenic	40.0%
!!!	TCCGTTTGGCTTTTGTTTTC+TGG	-	chr3.2:76725396-76725415	None:intergenic	40.0%
!!!	TTGGCTGTTTTAACTTGCGT+AGG	-	chr3.2:76725109-76725128	None:intergenic	40.0%
	ACGGTCTCTTCAATACAGGA+TGG	-	chr3.2:76725021-76725040	None:intergenic	45.0%
	GAGACAGCTCTTGGTTTCTT+AGG	-	chr3.2:76725169-76725188	None:intergenic	45.0%
	TCTCGAAGGGGTAAGAAGAT+TGG	+	chr3.2:76725325-76725344	MS.gene06751:CDS	45.0%
	CAGCCAAGACGCACAAGAAA+AGG	+	chr3.2:76725122-76725141	MS.gene06751:CDS	50.0%
	CTGTTTCAAGCTCAGCTGCT+CGG	+	chr3.2:76724960-76724979	MS.gene06751:CDS	50.0%
	ACCAAGAGCTGTCTCCACTG+AGG	+	chr3.2:76725174-76725193	MS.gene06751:CDS	55.0%
	TCCTCAGTGGAGACAGCTCT+TGG	-	chr3.2:76725178-76725197	None:intergenic	55.0%
	TCAGCTGCTCGGACTCCACT+AGG	+	chr3.2:76724971-76724990	MS.gene06751:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	76724936	76725459	76724936	ID=MS.gene06751
chr3.2	mRNA	76724936	76725459	76724936	ID=MS.gene06751.t1;Parent=MS.gene06751
chr3.2	exon	76724936	76725459	76724936	ID=MS.gene06751.t1.exon1;Parent=MS.gene06751.t1
chr3.2	CDS	76724936	76725459	76724936	ID=cds.MS.gene06751.t1;Parent=MS.gene06751.t1

Gene Sequence

>MS.gene06751

GCAAACTTGGTATGGGAGTTGTGACTGTTTCAAGCTCAGCTGCTCGGACTCCACTAGGATTGAATACAAAGTTTTCAACTCACCATCCTGTATTGAAGAGACCGTTAACGGTAGCATTTAAAGGAGATAAACAGAATGACACAGCGTTGGTTGCGACTCAAGAGAAAATTCCTACGCAAGTTAAAACAGCCAAGACGCACAAGAAAAGGATAGCAAAAACGAATAAACTACCTAAGAAACCAAGAGCTGTCTCCACTGAGGAAAATTTTCCATCTTCATTGGATGTGGATTATAACGAAGCTGCTGCCATACTTGAAAATATATACAAACTTAGCCCGACAACCGATATATGTGATGCAGATTATATAGAACGTAAGATCAAAAGAGTCTCTCGAAGGGGTAAGAAGATTGGTGATGTAAGTAAAGAGGATTTAAACAGTGATAGGGTGGTCCGAAACCAGAAAACAAAAGCCAAACGGATGAATCTTGATGAGAGGATTGCGTTAAAAAACGAGCGACAATAG

Protein sequence

>MS.gene06751.t1

ANLVWELULFQAQLLGLHUDUIQSFQLTILYURDRURUHLKEINRMTQRWLRLKRKFLRKLKQPRRTRKGUQKRINYLRNQELSPLRKIFHLHWMWIITKLLPYLKIYTNLARQPIYVMQIIUNVRSKESLEGVRRLVMUVKRIUTVIGWSETRKQKPNGUILMRGLRUKTSDN