Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08782.t1 | XP_024628489.1 | 99.2 | 131 | 1 | 0 | 1 | 131 | 307 | 437 | 1.20E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08782.t1 | Q93W93 | 61.8 | 131 | 46 | 2 | 1 | 131 | 308 | 434 | 3.7e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08782.t1 | G7LHY4 | 99.2 | 131 | 1 | 0 | 1 | 131 | 307 | 437 | 8.6e-59 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene08782 | 0.800061 | 1.72E-48 | -1.69E-46 |
| MS.gene049159 | MS.gene08782 | 0.804136 | 2.50E-49 | -1.69E-46 |
| MS.gene049230 | MS.gene08782 | 0.865187 | 7.08E-65 | -1.69E-46 |
| MS.gene049378 | MS.gene08782 | 0.879197 | 1.52E-69 | -1.69E-46 |
| MS.gene049669 | MS.gene08782 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene08782 | 0.804524 | 2.07E-49 | -1.69E-46 |
| MS.gene049910 | MS.gene08782 | 0.815969 | 7.04E-52 | -1.69E-46 |
| MS.gene050105 | MS.gene08782 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene08782 | 0.833628 | 4.78E-56 | -1.69E-46 |
| MS.gene050227 | MS.gene08782 | 0.854605 | 1.10E-61 | -1.69E-46 |
| MS.gene050228 | MS.gene08782 | 0.845129 | 4.92E-59 | -1.69E-46 |
| MS.gene050303 | MS.gene08782 | 0.828338 | 9.50E-55 | -1.69E-46 |
| MS.gene050330 | MS.gene08782 | -0.807136 | 5.86E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene08782 | 0.844816 | 5.98E-59 | -1.69E-46 |
| MS.gene050605 | MS.gene08782 | 0.835482 | 1.64E-56 | -1.69E-46 |
| MS.gene050623 | MS.gene08782 | -0.882119 | 1.36E-70 | -1.69E-46 |
| MS.gene050642 | MS.gene08782 | 0.886049 | 4.79E-72 | -1.69E-46 |
| MS.gene050680 | MS.gene08782 | 0.808744 | 2.66E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene08782 | 0.813753 | 2.18E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene08782 | 0.81434 | 1.62E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene08782 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene08782 | 0.830937 | 2.22E-55 | -1.69E-46 |
| MS.gene050820 | MS.gene08782 | 0.8085 | 3.00E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene08782 | 0.848186 | 7.19E-60 | -1.69E-46 |
| MS.gene051243 | MS.gene08782 | 0.808825 | 2.56E-50 | -1.69E-46 |
| MS.gene051349 | MS.gene08782 | 0.810948 | 8.94E-51 | -1.69E-46 |
| MS.gene051368 | MS.gene08782 | 0.813718 | 2.22E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene08782 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene051452 | MS.gene08782 | 0.816289 | 5.97E-52 | -1.69E-46 |
| MS.gene051535 | MS.gene08782 | 0.840022 | 1.12E-57 | -1.69E-46 |
| MS.gene051860 | MS.gene08782 | 0.832551 | 8.86E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene08782 | 0.895432 | 9.64E-76 | -1.69E-46 |
| MS.gene052012 | MS.gene08782 | 0.815501 | 8.95E-52 | -1.69E-46 |
| MS.gene052077 | MS.gene08782 | 0.85464 | 1.08E-61 | -1.69E-46 |
| MS.gene052176 | MS.gene08782 | 0.85723 | 1.88E-62 | -1.69E-46 |
| MS.gene052309 | MS.gene08782 | -0.843592 | 1.27E-58 | -1.69E-46 |
| MS.gene052333 | MS.gene08782 | 0.815161 | 1.07E-51 | -1.69E-46 |
| MS.gene052389 | MS.gene08782 | 0.83081 | 2.38E-55 | -1.69E-46 |
| MS.gene052426 | MS.gene08782 | 0.826634 | 2.44E-54 | -1.69E-46 |
| MS.gene052954 | MS.gene08782 | 0.805381 | 1.37E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene08782 | 0.833491 | 5.17E-56 | -1.69E-46 |
| MS.gene053166 | MS.gene08782 | 0.813915 | 2.01E-51 | -1.69E-46 |
| MS.gene053494 | MS.gene08782 | 0.822236 | 2.64E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene08782 | 0.819309 | 1.24E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene08782 | 0.809191 | 2.14E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene08782 | 0.841664 | 4.14E-58 | -1.69E-46 |
| MS.gene054468 | MS.gene08782 | 0.80383 | 2.89E-49 | -1.69E-46 |
| MS.gene054542 | MS.gene08782 | 0.80095 | 1.13E-48 | -1.69E-46 |
| MS.gene054559 | MS.gene08782 | 0.802787 | 4.75E-49 | -1.69E-46 |
| MS.gene054657 | MS.gene08782 | 0.827067 | 1.92E-54 | -1.69E-46 |
| MS.gene054658 | MS.gene08782 | 0.803589 | 3.24E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene08782 | 0.845637 | 3.59E-59 | -1.69E-46 |
| MS.gene054983 | MS.gene08782 | 0.811218 | 7.81E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene08782 | 0.854188 | 1.45E-61 | -1.69E-46 |
| MS.gene055623 | MS.gene08782 | 0.804471 | 2.13E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene08782 | 0.890037 | 1.41E-73 | -1.69E-46 |
| MS.gene055818 | MS.gene08782 | 0.801308 | 9.57E-49 | -1.69E-46 |
| MS.gene056087 | MS.gene08782 | 0.80288 | 4.55E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene08782 | 0.847375 | 1.20E-59 | -1.69E-46 |
| MS.gene056979 | MS.gene08782 | 0.881528 | 2.23E-70 | -1.69E-46 |
| MS.gene057026 | MS.gene08782 | 0.81969 | 1.02E-52 | -1.69E-46 |
| MS.gene057156 | MS.gene08782 | -0.805924 | 1.06E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene08782 | 0.829582 | 4.75E-55 | -1.69E-46 |
| MS.gene057639 | MS.gene08782 | 0.805589 | 1.24E-49 | -1.69E-46 |
| MS.gene058139 | MS.gene08782 | 0.831327 | 1.78E-55 | -1.69E-46 |
| MS.gene058142 | MS.gene08782 | 0.814623 | 1.40E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene08782 | 0.842689 | 2.22E-58 | -1.69E-46 |
| MS.gene058399 | MS.gene08782 | 0.853046 | 3.09E-61 | -1.69E-46 |
| MS.gene058435 | MS.gene08782 | 0.857501 | 1.56E-62 | -1.69E-46 |
| MS.gene058548 | MS.gene08782 | 0.827891 | 1.22E-54 | -1.69E-46 |
| MS.gene058549 | MS.gene08782 | 0.818507 | 1.89E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene08782 | 0.818911 | 1.53E-52 | -1.69E-46 |
| MS.gene059161 | MS.gene08782 | 0.821089 | 4.86E-53 | -1.69E-46 |
| MS.gene059247 | MS.gene08782 | 0.800706 | 1.27E-48 | -1.69E-46 |
| MS.gene059249 | MS.gene08782 | 0.819189 | 1.32E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene08782 | 0.804365 | 2.24E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene08782 | 0.804906 | 1.72E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene08782 | 0.825407 | 4.77E-54 | -1.69E-46 |
| MS.gene059710 | MS.gene08782 | 0.811036 | 8.56E-51 | -1.69E-46 |
| MS.gene05979 | MS.gene08782 | 0.832594 | 8.65E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene08782 | 0.811527 | 6.69E-51 | -1.69E-46 |
| MS.gene060084 | MS.gene08782 | 0.811206 | 7.86E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene08782 | 0.839559 | 1.47E-57 | -1.69E-46 |
| MS.gene060322 | MS.gene08782 | 0.827218 | 1.77E-54 | -1.69E-46 |
| MS.gene060572 | MS.gene08782 | 0.830178 | 3.40E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene08782 | 0.848074 | 7.73E-60 | -1.69E-46 |
| MS.gene060685 | MS.gene08782 | 0.84312 | 1.70E-58 | -1.69E-46 |
| MS.gene060802 | MS.gene08782 | 0.834613 | 2.71E-56 | -1.69E-46 |
| MS.gene060803 | MS.gene08782 | 0.855798 | 4.95E-62 | -1.69E-46 |
| MS.gene060879 | MS.gene08782 | 0.807963 | 3.91E-50 | -1.69E-46 |
| MS.gene060942 | MS.gene08782 | 0.831455 | 1.65E-55 | -1.69E-46 |
| MS.gene060998 | MS.gene08782 | 0.821125 | 4.77E-53 | -1.69E-46 |
| MS.gene061002 | MS.gene08782 | 0.809741 | 1.63E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene08782 | -0.837669 | 4.54E-57 | -1.69E-46 |
| MS.gene061114 | MS.gene08782 | -0.809066 | 2.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08782.t1 | MTR_8g085600 | 99.237 | 131 | 1 | 0 | 1 | 131 | 307 | 437 | 2.79e-89 | 266 |
| MS.gene08782.t1 | MTR_4g114110 | 69.697 | 132 | 39 | 1 | 1 | 131 | 311 | 442 | 6.80e-58 | 185 |
| MS.gene08782.t1 | MTR_3g083700 | 60.870 | 69 | 23 | 2 | 31 | 98 | 53 | 118 | 1.28e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08782.t1 | AT1G55270 | 69.466 | 131 | 36 | 2 | 1 | 131 | 182 | 308 | 1.13e-59 | 186 |
| MS.gene08782.t1 | AT1G55270 | 69.466 | 131 | 36 | 2 | 1 | 131 | 308 | 434 | 1.63e-58 | 187 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGCTCTTGTTTCACTTAA+TGG | 0.104977 | 4.2:+12824581 | MS.gene08782:CDS |
| GGTCTTTAGTTAGGAATTTA+TGG | 0.197065 | 4.2:+12824723 | MS.gene08782:CDS |
| ATCGATAGCAGGCTTCATTT+AGG | 0.362076 | 4.2:+12824536 | MS.gene08782:CDS |
| CACCTCTGGGAAAATATTGC+TGG | 0.367840 | 4.2:+12824686 | MS.gene08782:CDS |
| TAAACTCAAAGTCTATGATA+TGG | 0.375642 | 4.2:+12824490 | MS.gene08782:CDS |
| ATGATATGGCGACGGATTCA+TGG | 0.409397 | 4.2:+12824504 | MS.gene08782:CDS |
| AATCCAAGTATCTCACTTAA+CGG | 0.412505 | 4.2:+12824434 | MS.gene08782:CDS |
| TTGAAAACAATCCTCACCTC+TGG | 0.437794 | 4.2:+12824672 | MS.gene08782:CDS |
| CTTTGAGTTTACAGCCGTCT+TGG | 0.451770 | 4.2:-12824478 | None:intergenic |
| GTCCTGTCAGGTCTTTAGTT+AGG | 0.455869 | 4.2:+12824714 | MS.gene08782:CDS |
| CTGGGAAAATATTGCTGGAA+AGG | 0.473920 | 4.2:+12824691 | MS.gene08782:CDS |
| CAACAATATGAGCATCAGTC+TGG | 0.486023 | 4.2:+12824622 | MS.gene08782:CDS |
| TCTCCGTTAAGTGAGATACT+TGG | 0.486498 | 4.2:-12824437 | None:intergenic |
| GGAAGAAGTTTATCGATAGC+AGG | 0.495335 | 4.2:+12824525 | MS.gene08782:CDS |
| AATTTATGGTCTACTATCGC+AGG | 0.517084 | 4.2:+12824737 | MS.gene08782:CDS |
| TTGCTGGAAAGGGTCCTGTC+AGG | 0.517356 | 4.2:+12824702 | MS.gene08782:CDS |
| AGTAAACAATGGAATGGTCG+TGG | 0.529726 | 4.2:+12824403 | None:intergenic |
| TGGGAAAATATTGCTGGAAA+GGG | 0.544190 | 4.2:+12824692 | MS.gene08782:CDS |
| TTCCTAACTAAAGACCTGAC+AGG | 0.558546 | 4.2:-12824716 | None:intergenic |
| TATGCACTTGATTGCCAAGA+CGG | 0.568382 | 4.2:+12824464 | MS.gene08782:CDS |
| TGAAAACAATCCTCACCTCT+GGG | 0.580142 | 4.2:+12824673 | MS.gene08782:CDS |
| TGGTCTACTATCGCAGGGCG+CGG | 0.607872 | 4.2:+12824743 | MS.gene08782:CDS |
| ACAATGGAATGGTCGTGGGA+TGG | 0.614216 | 4.2:+12824408 | None:intergenic |
| TCTACTATCGCAGGGCGCGG+TGG | 0.621549 | 4.2:+12824746 | MS.gene08782:CDS |
| GTAAACAATGGAATGGTCGT+GGG | 0.628265 | 4.2:+12824404 | None:intergenic |
| CAAAGTCTATGATATGGCGA+CGG | 0.642307 | 4.2:+12824496 | MS.gene08782:CDS |
| ATTTATGGTCTACTATCGCA+GGG | 0.652108 | 4.2:+12824738 | MS.gene08782:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAACTCAAAGTCTATGATA+TGG | + | chr4.2:12824490-12824509 | MS.gene08782:CDS | 25.0% |
| AATCCAAGTATCTCACTTAA+CGG | + | chr4.2:12824434-12824453 | MS.gene08782:CDS | 30.0% | |
| GGTCTTTAGTTAGGAATTTA+TGG | + | chr4.2:12824723-12824742 | MS.gene08782:CDS | 30.0% | |
| AATTTATGGTCTACTATCGC+AGG | + | chr4.2:12824737-12824756 | MS.gene08782:CDS | 35.0% | |
| ATTTATGGTCTACTATCGCA+GGG | + | chr4.2:12824738-12824757 | MS.gene08782:CDS | 35.0% | |
| TGGGAAAATATTGCTGGAAA+GGG | + | chr4.2:12824692-12824711 | MS.gene08782:CDS | 35.0% | |
| ATCGATAGCAGGCTTCATTT+AGG | + | chr4.2:12824536-12824555 | MS.gene08782:CDS | 40.0% | |
| CAAAGTCTATGATATGGCGA+CGG | + | chr4.2:12824496-12824515 | MS.gene08782:CDS | 40.0% | |
| CAACAATATGAGCATCAGTC+TGG | + | chr4.2:12824622-12824641 | MS.gene08782:CDS | 40.0% | |
| CTGGGAAAATATTGCTGGAA+AGG | + | chr4.2:12824691-12824710 | MS.gene08782:CDS | 40.0% | |
| GGAAGAAGTTTATCGATAGC+AGG | + | chr4.2:12824525-12824544 | MS.gene08782:CDS | 40.0% | |
| TATGCACTTGATTGCCAAGA+CGG | + | chr4.2:12824464-12824483 | MS.gene08782:CDS | 40.0% | |
| TCTCCGTTAAGTGAGATACT+TGG | - | chr4.2:12824440-12824459 | None:intergenic | 40.0% | |
| TGAAAACAATCCTCACCTCT+GGG | + | chr4.2:12824673-12824692 | MS.gene08782:CDS | 40.0% | |
| TTCCTAACTAAAGACCTGAC+AGG | - | chr4.2:12824719-12824738 | None:intergenic | 40.0% | |
| TTGAAAACAATCCTCACCTC+TGG | + | chr4.2:12824672-12824691 | MS.gene08782:CDS | 40.0% | |
| ! | GCAGCTCTTGTTTCACTTAA+TGG | + | chr4.2:12824581-12824600 | MS.gene08782:CDS | 40.0% |
| ! | TTCCAGCAATATTTTCCCAG+AGG | - | chr4.2:12824691-12824710 | None:intergenic | 40.0% |
| ! | TTGTTTTCAACGCGTCTGTT+TGG | - | chr4.2:12824662-12824681 | None:intergenic | 40.0% |
| GTCCTGTCAGGTCTTTAGTT+AGG | + | chr4.2:12824714-12824733 | MS.gene08782:CDS | 45.0% | |
| ! | ATGATATGGCGACGGATTCA+TGG | + | chr4.2:12824504-12824523 | MS.gene08782:CDS | 45.0% |
| ! | CACCTCTGGGAAAATATTGC+TGG | + | chr4.2:12824686-12824705 | MS.gene08782:CDS | 45.0% |
| ! | CTTTGAGTTTACAGCCGTCT+TGG | - | chr4.2:12824481-12824500 | None:intergenic | 45.0% |
| ! | GCAATATTTTCCCAGAGGTG+AGG | - | chr4.2:12824686-12824705 | None:intergenic | 45.0% |
| TTGCTGGAAAGGGTCCTGTC+AGG | + | chr4.2:12824702-12824721 | MS.gene08782:CDS | 55.0% | |
| TGGTCTACTATCGCAGGGCG+CGG | + | chr4.2:12824743-12824762 | MS.gene08782:CDS | 60.0% | |
| TCTACTATCGCAGGGCGCGG+TGG | + | chr4.2:12824746-12824765 | MS.gene08782:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 12824416 | 12824811 | 12824416 | ID=MS.gene08782 |
| chr4.2 | mRNA | 12824416 | 12824811 | 12824416 | ID=MS.gene08782.t1;Parent=MS.gene08782 |
| chr4.2 | exon | 12824416 | 12824811 | 12824416 | ID=MS.gene08782.t1.exon1;Parent=MS.gene08782.t1 |
| chr4.2 | CDS | 12824416 | 12824811 | 12824416 | ID=cds.MS.gene08782.t1;Parent=MS.gene08782.t1 |
| Gene Sequence |
| Protein sequence |
