AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24570.t1 MTR_1g016170 94.527 201 11 0 2 202 105 305 1.25e-129 367
MS.gene24570.t1 MTR_3g050550 80.233 86 17 0 2 87 9 94 2.63e-42 138
MS.gene24570.t1 MTR_3g050610 84.375 96 12 1 2 97 55 147 2.04e-41 137
MS.gene24570.t1 MTR_4g126350 44.037 109 40 4 5 113 128 215 2.22e-17 79.0
MS.gene24570.t1 MTR_2g038720 37.321 209 88 7 5 200 110 288 1.86e-16 76.3
MS.gene24570.t1 MTR_4g121110 54.237 59 27 0 70 128 16 74 4.78e-16 72.0
MS.gene24570.t1 MTR_7g112330 32.258 124 62 2 84 202 22 128 3.53e-13 64.3
MS.gene24570.t1 MTR_5g020230 57.692 52 19 1 72 120 107 158 2.35e-12 63.9
MS.gene24570.t1 MTR_1g094930 56.098 41 18 0 79 119 142 182 3.10e-11 60.8
MS.gene24570.t1 MTR_1g094930 56.098 41 18 0 79 119 237 277 5.01e-11 61.2
MS.gene24570.t1 MTR_8g077510 63.158 38 14 0 83 120 124 161 5.41e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24570.t1 AT5G56860 42.748 131 48 6 5 117 142 263 6.64e-20 86.7
MS.gene24570.t1 AT5G49300 66.667 45 15 0 80 124 32 76 2.35e-17 75.5
MS.gene24570.t1 AT4G26150 43.363 113 33 4 5 115 147 230 8.15e-17 77.8
MS.gene24570.t1 AT5G26930 68.293 41 13 0 81 121 23 63 4.22e-16 71.6
MS.gene24570.t1 AT3G06740 66.667 42 14 0 81 122 38 79 9.30e-15 68.9
MS.gene24570.t1 AT4G16141 68.293 41 13 0 81 121 34 74 1.33e-14 69.7
MS.gene24570.t1 AT3G16870 57.143 56 20 1 81 136 39 90 2.53e-14 68.6
MS.gene24570.t1 AT4G36620 58.333 48 20 0 80 127 71 118 1.59e-12 64.3
MS.gene24570.t1 AT2G18380 68.293 41 12 1 84 123 92 132 3.57e-12 63.2
MS.gene24570.t1 AT5G25830 55.556 45 20 0 75 119 210 254 1.59e-11 62.8
MS.gene24570.t1 AT3G50870 65.789 38 13 0 83 120 151 188 2.43e-11 62.0
MS.gene24570.t1 AT1G08010 57.778 45 18 1 75 119 212 255 5.05e-11 60.8
MS.gene24570.t1 AT1G08010 57.778 45 18 1 75 119 212 255 5.05e-11 60.8
MS.gene24570.t1 AT1G08010 57.778 45 18 1 75 119 212 255 5.05e-11 60.8
MS.gene24570.t1 AT2G45050 46.000 50 27 0 70 119 165 214 6.33e-11 60.5
MS.gene24570.t1 AT1G08000 52.273 44 21 0 76 119 210 253 8.80e-11 60.5
MS.gene24570.t1 AT1G08000 52.273 44 21 0 76 119 210 253 8.80e-11 60.5

Find 49 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGTTTGGTCTCATTAT+TGG 0.223553 1.2:-6339317 None:intergenic
ATGATGAAACCTAGTAATTA+TGG 0.301261 1.2:+6339383 MS.gene24570:CDS
TTGTTACCGCTGTTACTACT+AGG 0.336848 1.2:-6339449 None:intergenic
TGTTACTACTAGGGCTTGTT+TGG 0.337618 1.2:-6339439 None:intergenic
CACTCCTCCATAGTGGGGTA+TGG 0.338618 1.2:-6339505 None:intergenic
GTCCAAGAAATCAAAATATA+AGG 0.340331 1.2:+6339411 MS.gene24570:CDS
TTTGCAATGCTTGTGGAATC+AGG 0.357272 1.2:+6340129 MS.gene24570:CDS
TCATCATAATCATAATCTAA+TGG 0.373491 1.2:-6340341 None:intergenic
AGCAACGGCGGCACGACTTC+TGG 0.401059 1.2:+6340367 MS.gene24570:CDS
TAATTACTAGGTTTCATCAT+TGG 0.409747 1.2:-6339380 None:intergenic
GTGGCTGCAATATTGTTGTT+TGG 0.421269 1.2:-6339329 None:intergenic
GGCTGAAGCTGCCAATGGTT+TGG 0.422143 1.2:+6340175 MS.gene24570:CDS
CGAATTACCTTTGGACCCAT+AGG 0.444966 1.2:-6339530 None:intergenic
GGACTAGTACCATAATTACT+AGG 0.449247 1.2:-6339392 None:intergenic
ACTATGGAGGAGTGGCCCTA+TGG 0.450797 1.2:+6339514 MS.gene24570:CDS
CTTCTGGTGGATATATCTTC+TGG 0.451597 1.2:+6340383 MS.gene24570:CDS
ACAGCGGTAACAACACCGTT+AGG 0.456940 1.2:+6339459 MS.gene24570:CDS
CTATGGAGGAGTGGCCCTAT+GGG 0.464867 1.2:+6339515 MS.gene24570:CDS
CTTGATAGCTTTGTCAGTAG+TGG 0.482210 1.2:-6339348 None:intergenic
ACTCCTCCATAGTGGGGTAT+GGG 0.482841 1.2:-6339504 None:intergenic
GTACATCCCATACCCCACTA+TGG 0.488777 1.2:+6339498 MS.gene24570:CDS
TTCTTAGTCTTGAATTGTGT+AGG 0.495944 1.2:-6340236 None:intergenic
TGGTCTCATTATTGGAGTTG+TGG 0.515285 1.2:-6339309 None:intergenic
TCAGGCAGAGAAAGGCAAGA+AGG 0.520139 1.2:+6340147 MS.gene24570:CDS
CAGGCAGAGAAAGGCAAGAA+GGG 0.526577 1.2:+6340148 MS.gene24570:CDS
GAATTACCTTTGGACCCATA+GGG 0.528192 1.2:-6339529 None:intergenic
TGAGTCACGGGTCTGAGAGA+TGG 0.533532 1.2:+6339231 MS.gene24570:CDS
AGGGGATGTAGCCAAACCAT+TGG 0.539225 1.2:-6340186 None:intergenic
CAGTCACTTTGCAATGCTTG+TGG 0.543553 1.2:+6340122 MS.gene24570:intron
CAGCGGTAACAACACCGTTA+GGG 0.546032 1.2:+6339460 MS.gene24570:CDS
ACCATTGGCAGCTTCAGCCA+TGG 0.551217 1.2:-6340171 None:intergenic
TTGTGGAATCAGGCAGAGAA+AGG 0.551715 1.2:+6340139 MS.gene24570:CDS
TAGGGCCACTCCTCCATAGT+GGG 0.558056 1.2:-6339511 None:intergenic
TGTTACCGCTGTTACTACTA+GGG 0.563428 1.2:-6339448 None:intergenic
ATAGGGCCACTCCTCCATAG+TGG 0.566452 1.2:-6339512 None:intergenic
AGAAGTAGTTGAAGTTGTAG+AGG 0.579757 1.2:-6340276 None:intergenic
GAGAAAGGCAAGAAGGGCCA+TGG 0.585866 1.2:+6340154 MS.gene24570:CDS
GAGTGGCCCTATGGGTCCAA+AGG 0.590272 1.2:+6339523 MS.gene24570:CDS
GATGGATGTCTTCAAAGATG+AGG 0.603732 1.2:+6339249 MS.gene24570:CDS
TGATTATGATGAAGCAGCAA+CGG 0.614524 1.2:+6340352 MS.gene24570:CDS
GCCATGGCTGAAGCTGCCAA+TGG 0.619219 1.2:+6340170 MS.gene24570:CDS
AACGGCGGCACGACTTCTGG+TGG 0.640817 1.2:+6340370 MS.gene24570:CDS
ACAATCAGAACAAACCCTAA+CGG 0.645452 1.2:-6339474 None:intergenic
ACAATTCAAGACTAAGAACA+AGG 0.668290 1.2:+6340241 MS.gene24570:CDS
CATACCCCACTATGGAGGAG+TGG 0.671274 1.2:+6339506 MS.gene24570:CDS
CATCCCATACCCCACTATGG+AGG 0.682705 1.2:+6339501 MS.gene24570:CDS
TTATGATGAAGCAGCAACGG+CGG 0.694941 1.2:+6340355 MS.gene24570:CDS
AGGGCCACTCCTCCATAGTG+GGG 0.711722 1.2:-6339510 None:intergenic
ACAAGCCCTAGTAGTAACAG+CGG 0.724459 1.2:+6339443 MS.gene24570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATTAAGTTTAATTA+TGG - chr1.2:6340050-6340069 None:intergenic 10.0%
!! ATTAATATTTATTGTTCATA+AGG + chr1.2:6339693-6339712 MS.gene24570:intron 10.0%
!! TAATTAAAAAGATAAAATAG+AGG - chr1.2:6339877-6339896 None:intergenic 10.0%
!! TGATAAAATATTTGTATTAA+AGG - chr1.2:6339780-6339799 None:intergenic 10.0%
!! TTAATATTTATTGTTCATAA+GGG + chr1.2:6339694-6339713 MS.gene24570:intron 10.0%
!!! ATTAATATATTTTTTTTGTG+GGG - chr1.2:6339679-6339698 None:intergenic 10.0%
!!! GATATTAATAATTTTTGAGA+TGG - chr1.2:6339654-6339673 None:intergenic 15.0%
!!! TGTTTTTGTAAGAATTTTTA+TGG - chr1.2:6339746-6339765 None:intergenic 15.0%
!! ATTTGTATTAAAGGAAGTAA+AGG - chr1.2:6339771-6339790 None:intergenic 20.0%
!! TCATCATAATCATAATCTAA+TGG - chr1.2:6340344-6340363 None:intergenic 20.0%
!!! CGATAAATGATAAATTTTAG+TGG - chr1.2:6339620-6339639 None:intergenic 20.0%
!!! TACCTTATATTTTGATTTCT+TGG - chr1.2:6339416-6339435 None:intergenic 20.0%
! ACTATTCTTTCGTATTAAAG+TGG + chr1.2:6339571-6339590 MS.gene24570:intron 25.0%
! ATACGAAAGAATAGTGAATA+AGG - chr1.2:6339566-6339585 None:intergenic 25.0%
! ATGATGAAACCTAGTAATTA+TGG + chr1.2:6339383-6339402 MS.gene24570:CDS 25.0%
! GTCCAAGAAATCAAAATATA+AGG + chr1.2:6339411-6339430 MS.gene24570:CDS 25.0%
! TAAAAAGATAAAATAGAGGC+AGG - chr1.2:6339873-6339892 None:intergenic 25.0%
! TAATTACTAGGTTTCATCAT+TGG - chr1.2:6339383-6339402 None:intergenic 25.0%
! TAGTTAACATGAAAACATGT+TGG + chr1.2:6340097-6340116 MS.gene24570:intron 25.0%
!!! ATCTTAAGCACTTTAGTTTT+AGG - chr1.2:6340209-6340228 None:intergenic 25.0%
!!! TCTTAAGCACTTTAGTTTTA+GGG - chr1.2:6340208-6340227 None:intergenic 25.0%
AAAGAATAGTGAATAAGGCT+GGG - chr1.2:6339561-6339580 None:intergenic 30.0%
ACAATTCAAGACTAAGAACA+AGG + chr1.2:6340241-6340260 MS.gene24570:CDS 30.0%
TTCATAAGGGATGTCAAATA+TGG + chr1.2:6339707-6339726 MS.gene24570:intron 30.0%
! TTTAGTGGATCATAACAACA+AGG - chr1.2:6339605-6339624 None:intergenic 30.0%
!! TTCTTAGTCTTGAATTGTGT+AGG - chr1.2:6340239-6340258 None:intergenic 30.0%
!! TTGTTGTTTGGTCTCATTAT+TGG - chr1.2:6339320-6339339 None:intergenic 30.0%
!!! CTTAAGCACTTTAGTTTTAG+GGG - chr1.2:6340207-6340226 None:intergenic 30.0%
ACAATCAGAACAAACCCTAA+CGG - chr1.2:6339477-6339496 None:intergenic 35.0%
AGAAGTAGTTGAAGTTGTAG+AGG - chr1.2:6340279-6340298 None:intergenic 35.0%
GAAAGAATAGTGAATAAGGC+TGG - chr1.2:6339562-6339581 None:intergenic 35.0%
TGATTATGATGAAGCAGCAA+CGG + chr1.2:6340352-6340371 MS.gene24570:CDS 35.0%
TGGGATTAACGAATTACCTT+TGG - chr1.2:6339542-6339561 None:intergenic 35.0%
! GGACTAGTACCATAATTACT+AGG - chr1.2:6339395-6339414 None:intergenic 35.0%
GAATTACCTTTGGACCCATA+GGG - chr1.2:6339532-6339551 None:intergenic 40.0%
GATGGATGTCTTCAAAGATG+AGG + chr1.2:6339249-6339268 MS.gene24570:CDS 40.0%
TGGTCTCATTATTGGAGTTG+TGG - chr1.2:6339312-6339331 None:intergenic 40.0%
TGTTACCGCTGTTACTACTA+GGG - chr1.2:6339451-6339470 None:intergenic 40.0%
TTGTTACCGCTGTTACTACT+AGG - chr1.2:6339452-6339471 None:intergenic 40.0%
TTTGCAATGCTTGTGGAATC+AGG + chr1.2:6340129-6340148 MS.gene24570:CDS 40.0%
! CTTCTGGTGGATATATCTTC+TGG + chr1.2:6340383-6340402 MS.gene24570:CDS 40.0%
! GTGGCTGCAATATTGTTGTT+TGG - chr1.2:6339332-6339351 None:intergenic 40.0%
! TGTTACTACTAGGGCTTGTT+TGG - chr1.2:6339442-6339461 None:intergenic 40.0%
!! CTTGATAGCTTTGTCAGTAG+TGG - chr1.2:6339351-6339370 None:intergenic 40.0%
ACAAGCCCTAGTAGTAACAG+CGG + chr1.2:6339443-6339462 MS.gene24570:CDS 45.0%
CGAATTACCTTTGGACCCAT+AGG - chr1.2:6339533-6339552 None:intergenic 45.0%
TTATGATGAAGCAGCAACGG+CGG + chr1.2:6340355-6340374 MS.gene24570:CDS 45.0%
TTGTGGAATCAGGCAGAGAA+AGG + chr1.2:6340139-6340158 MS.gene24570:CDS 45.0%
! CAGTCACTTTGCAATGCTTG+TGG + chr1.2:6340122-6340141 MS.gene24570:intron 45.0%
!!! ATATTAATATATTTTTTTTG+TGG - chr1.2:6339681-6339700 None:intergenic 5.0%
!!! TAATTTTTATTATTATATCA+CGG + chr1.2:6339963-6339982 MS.gene24570:intron 5.0%
!!! TATTAATATATTTTTTTTGT+GGG - chr1.2:6339680-6339699 None:intergenic 5.0%
ACAGCGGTAACAACACCGTT+AGG + chr1.2:6339459-6339478 MS.gene24570:CDS 50.0%
AGGGGATGTAGCCAAACCAT+TGG - chr1.2:6340189-6340208 None:intergenic 50.0%
CAGCGGTAACAACACCGTTA+GGG + chr1.2:6339460-6339479 MS.gene24570:CDS 50.0%
CAGGCAGAGAAAGGCAAGAA+GGG + chr1.2:6340148-6340167 MS.gene24570:CDS 50.0%
GTACATCCCATACCCCACTA+TGG + chr1.2:6339498-6339517 MS.gene24570:CDS 50.0%
TCAGGCAGAGAAAGGCAAGA+AGG + chr1.2:6340147-6340166 MS.gene24570:CDS 50.0%
! ACTCCTCCATAGTGGGGTAT+GGG - chr1.2:6339507-6339526 None:intergenic 50.0%
ACCATTGGCAGCTTCAGCCA+TGG - chr1.2:6340174-6340193 None:intergenic 55.0%
ACTATGGAGGAGTGGCCCTA+TGG + chr1.2:6339514-6339533 MS.gene24570:CDS 55.0%
ATAGGGCCACTCCTCCATAG+TGG - chr1.2:6339515-6339534 None:intergenic 55.0%
CATACCCCACTATGGAGGAG+TGG + chr1.2:6339506-6339525 MS.gene24570:CDS 55.0%
CATCCCATACCCCACTATGG+AGG + chr1.2:6339501-6339520 MS.gene24570:CDS 55.0%
CTATGGAGGAGTGGCCCTAT+GGG + chr1.2:6339515-6339534 MS.gene24570:CDS 55.0%
GAGAAAGGCAAGAAGGGCCA+TGG + chr1.2:6340154-6340173 MS.gene24570:CDS 55.0%
GGCTGAAGCTGCCAATGGTT+TGG + chr1.2:6340175-6340194 MS.gene24570:CDS 55.0%
TAGGGCCACTCCTCCATAGT+GGG - chr1.2:6339514-6339533 None:intergenic 55.0%
TGAGTCACGGGTCTGAGAGA+TGG + chr1.2:6339231-6339250 MS.gene24570:CDS 55.0%
! CACTCCTCCATAGTGGGGTA+TGG - chr1.2:6339508-6339527 None:intergenic 55.0%
AGGGCCACTCCTCCATAGTG+GGG - chr1.2:6339513-6339532 None:intergenic 60.0%
GAGTGGCCCTATGGGTCCAA+AGG + chr1.2:6339523-6339542 MS.gene24570:CDS 60.0%
GCCATGGCTGAAGCTGCCAA+TGG + chr1.2:6340170-6340189 MS.gene24570:CDS 60.0%
AACGGCGGCACGACTTCTGG+TGG + chr1.2:6340370-6340389 MS.gene24570:CDS 65.0%
AGCAACGGCGGCACGACTTC+TGG + chr1.2:6340367-6340386 MS.gene24570:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 6339230 6340418 6339230 ID=MS.gene24570
chr1.2 mRNA 6339230 6340418 6339230 ID=MS.gene24570.t1;Parent=MS.gene24570
chr1.2 exon 6339230 6339544 6339230 ID=MS.gene24570.t1.exon1;Parent=MS.gene24570.t1
chr1.2 CDS 6339230 6339544 6339230 ID=cds.MS.gene24570.t1;Parent=MS.gene24570.t1
chr1.2 exon 6340125 6340418 6340125 ID=MS.gene24570.t1.exon2;Parent=MS.gene24570.t1
chr1.2 CDS 6340125 6340418 6340125 ID=cds.MS.gene24570.t1;Parent=MS.gene24570.t1
Gene Sequence

>MS.gene24570

ATGAGTCACGGGTCTGAGAGATGGATGTCTTCAAAGATGAGGTTGATGAATAAAATGATCAACAAAACTGCTACTGTTGCCACAACTCCAATAATGAGACCAAACAACAATATTGCAGCCACTACTGACAAAGCTATCAAGACAACAACTCCAATGATGAAACCTAGTAATTATGGTACTAGTCCAAGAAATCAAAATATAAGGTACAACCAAACAAGCCCTAGTAGTAACAGCGGTAACAACACCGTTAGGGTTTGTTCTGATTGTAGTACATCCCATACCCCACTATGGAGGAGTGGCCCTATGGGTCCAAAGGTAATTCGTTAATCCCAGCCTTATTCACTATTCTTTCGTATTAAAGTGGTAGAGTGACCTTGTTGTTATGATCCACTAAAATTTATCATTTATCGCTTCAATTCGTCCATCTCAAAAATTATTAATATCATCCCCACAAAAAAAATATATTAATATTTATTGTTCATAAGGGATGTCAAATATGGAATTTTATCAAGACCATAAAAATTCTTACAAAAACAATCCTTTACTTCCTTTAATACAAATATTTTATCAAAAAAATGTAGTATTCGTAGTAAAAGTTGAATTCATAGAAACGTCAAAATTAGAGATCAATTTAATTTTTCCTGCCTCTATTTTATCTTTTTAATTACAATATTTATAGTTTTGATATTCAAACATAAATAAGATATCAAGCTATATACAAGATCAATTAAAATAATTTTTATTATTATATCACGGTTTTAATTAGTCGAATATTTCGATAGATGATAGATCACACTAGAGTCATGCAAGTATTATTCCATAATTAAACTTAATTTGATTGATACTTGAATCATATCTAGTTATATATAGTTAACATGAAAACATGTTGGTGCAGTCACTTTGCAATGCTTGTGGAATCAGGCAGAGAAAGGCAAGAAGGGCCATGGCTGAAGCTGCCAATGGTTTGGCTACATCCCCTAAAACTAAAGTGCTTAAGATAAAGAAGCCTACACAATTCAAGACTAAGAACAAGGCTTCTACTAGTACCTCTACAACTTCAACTACTTCTGCTAGCTCATCATCTCAAGATGTGAAGAAACTTGAATCTTTTCCATTAGATTATGATTATGATGAAGCAGCAACGGCGGCACGACTTCTGGTGGATATATCTTCTGGTGTTACTTATTGA

Protein sequence

>MS.gene24570.t1

MSHGSERWMSSKMRLMNKMINKTATVATTPIMRPNNNIAATTDKAIKTTTPMMKPSNYGTSPRNQNIRYNQTSPSSNSGNNTVRVCSDCSTSHTPLWRSGPMGPKSLCNACGIRQRKARRAMAEAANGLATSPKTKVLKIKKPTQFKTKNKASTSTSTTSTTSASSSSQDVKKLESFPLDYDYDEAATAARLLVDISSGVTY