AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24642


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24642.t1 MTR_7g104020 100.000 168 0 0 1 168 1 168 2.79e-118 331
MS.gene24642.t1 MTR_7g104020 98.246 171 0 1 1 168 1 171 3.32e-116 326
MS.gene24642.t1 MTR_2g085025 69.032 155 46 1 14 166 20 174 6.12e-63 191
MS.gene24642.t1 MTR_1g492930 59.494 158 61 1 14 168 4 161 6.71e-59 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24642.t1 AT2G36410 77.564 156 34 1 10 165 38 192 1.68e-84 247
MS.gene24642.t1 AT2G36410 76.101 159 34 2 10 165 38 195 2.67e-82 241
MS.gene24642.t1 AT3G09980 82.911 158 27 0 9 166 21 178 1.76e-81 239
MS.gene24642.t1 AT3G52920 80.137 146 29 0 19 164 17 162 4.51e-81 238
MS.gene24642.t1 AT3G52920 78.523 149 29 1 19 164 17 165 7.78e-79 232
MS.gene24642.t1 AT5G03660 72.785 158 43 0 6 163 12 169 1.21e-65 198
MS.gene24642.t1 AT2G27740 60.526 152 58 1 14 163 19 170 2.87e-62 190
MS.gene24642.t1 AT2G36410 76.415 106 24 1 10 115 38 142 2.98e-54 169
MS.gene24642.t1 AT5G03660 70.504 139 41 0 6 144 12 150 8.90e-54 167
MS.gene24642.t1 AT3G52900 53.659 164 72 2 6 166 2 164 1.62e-52 165
MS.gene24642.t1 AT5G03660 62.025 158 38 1 6 163 12 147 9.39e-47 150
MS.gene24642.t1 AT2G36355 50.331 151 75 0 16 166 5 155 5.15e-45 145
MS.gene24642.t1 AT2G27740 57.692 104 42 1 14 115 19 122 8.57e-39 129
MS.gene24642.t1 AT2G36355 58.621 87 36 0 80 166 27 113 8.20e-31 108
MS.gene24642.t1 AT2G36355 58.333 84 35 0 83 166 1 84 5.65e-30 105
MS.gene24642.t1 AT2G36355 46.000 100 54 0 16 115 5 104 1.05e-22 88.2

Find 31 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTGGTTGTACAGAATTC+AGG 0.260998 7.2:-8801479 MS.gene24642:CDS
ACAGCTAATTACTAAATTAA+TGG 0.284229 7.2:-8800291 MS.gene24642:CDS
AAGCTTTCAATGAAAAGAAT+AGG 0.336763 7.2:-8800322 MS.gene24642:CDS
AGTGAAACTTCTTTCCTCAT+TGG 0.356625 7.2:+8800552 None:intergenic
TAACCTAGCTCACCTTCTTC+TGG 0.386647 7.2:+8800472 None:intergenic
AGCTTTCAATGAAAAGAATA+GGG 0.424472 7.2:-8800321 MS.gene24642:CDS
CTTTCCTCATTGGATCTGCA+AGG 0.450818 7.2:+8800562 None:intergenic
AGAAATTGAAAAGAAGAAGA+TGG 0.462319 7.2:-8801372 MS.gene24642:CDS
CTGAATTCTGTACAACCAAC+TGG 0.468188 7.2:+8801480 None:intergenic
AAGATTGAGGATGAAGAAGC+TGG 0.477057 7.2:-8799550 MS.gene24642:CDS
GCTAAATCATTGCATTTGCA+TGG 0.478899 7.2:+8799503 None:intergenic
GACAAGGTTCAGTTTCAGTT+GGG 0.484764 7.2:-8801341 MS.gene24642:CDS
TTTCCTCATTGGATCTGCAA+GGG 0.489503 7.2:+8800563 None:intergenic
AGACAAGGTTCAGTTTCAGT+TGG 0.495018 7.2:-8801342 MS.gene24642:CDS
AGTTGGTTGTACAGAATTCA+GGG 0.496303 7.2:-8801478 MS.gene24642:CDS
TGGTCATACCTGCCAGAAGA+AGG 0.536387 7.2:-8800484 MS.gene24642:intron
CTTTAGCAGTCACTTGTCAA+AGG 0.542571 7.2:-8801447 MS.gene24642:CDS
CTAGCTCACCTTCTTCTGGC+AGG 0.555949 7.2:+8800476 None:intergenic
TTCTGGCAGGTATGACCAAG+TGG 0.569790 7.2:+8800489 None:intergenic
TGTTGATGTGATTAGCTGGT+GGG 0.572662 7.2:-8799582 MS.gene24642:intron
GTTGATGTGATTAGCTGGTG+GGG 0.573033 7.2:-8799581 MS.gene24642:intron
AGGAAGATGAAGAGATGTCA+AGG 0.586395 7.2:-8801427 MS.gene24642:CDS
AACAAAGAATTAAAGCCACT+TGG 0.593321 7.2:-8800504 MS.gene24642:CDS
TTGATGTGATTAGCTGGTGG+GGG 0.596450 7.2:-8799580 MS.gene24642:intron
GCTAATTACTAAATTAATGG+AGG 0.597346 7.2:-8800288 MS.gene24642:intron
CAAAATATAAACCTCACGAA+TGG 0.615296 7.2:+8801286 None:intergenic
ACGTCTTGCTACCATTCGTG+AGG 0.618633 7.2:-8801297 MS.gene24642:intron
GAAGATGGAAGTTAGAGACA+AGG 0.621610 7.2:-8801357 MS.gene24642:CDS
AAGCCCTTGCAGATCCAATG+AGG 0.655089 7.2:-8800566 MS.gene24642:CDS
AAGCAACAGTAACAACCAGT+TGG 0.659075 7.2:-8801495 MS.gene24642:CDS
ATTGAGGATGAAGAAGCTGG+AGG 0.703230 7.2:-8799547 MS.gene24642:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGGAAAAAAAAAAATAA+TGG + chr7.2:8800343-8800362 None:intergenic 10.0%
!! AAATCTAAGAAGAAAAAAAA+AGG + chr7.2:8799760-8799779 None:intergenic 15.0%
!! AACATAAGTACAAAAATTTA+AGG - chr7.2:8799882-8799901 MS.gene24642:intron 15.0%
!! AATTCAATTTCAAATTTCAA+TGG + chr7.2:8800636-8800655 None:intergenic 15.0%
!! GAATATACATAAATATCTTT+AGG - chr7.2:8801334-8801353 MS.gene24642:CDS 15.0%
!! TGTAATAATTTGATTAATTG+AGG - chr7.2:8800084-8800103 MS.gene24642:intron 15.0%
!!! TAAATTTTTGTACTTATGTT+TGG + chr7.2:8799882-8799901 None:intergenic 15.0%
!!! TTTTTTTCCAAAAATGAAAA+GGG + chr7.2:8799816-8799835 None:intergenic 15.0%
!! AAAAAAAAAAATAATGGCTG+AGG + chr7.2:8800337-8800356 None:intergenic 20.0%
!! ACAGCTAATTACTAAATTAA+TGG - chr7.2:8800727-8800746 MS.gene24642:intron 20.0%
!! ATACATATTACAAATGAGAA+TGG - chr7.2:8801185-8801204 MS.gene24642:intron 20.0%
!!! AAACTGTTAATTAGTGTTTT+TGG - chr7.2:8800586-8800605 MS.gene24642:intron 20.0%
!!! AACAAATTTTAGTTTCAAGT+TGG - chr7.2:8800925-8800944 MS.gene24642:intron 20.0%
!!! ATAAAATTGATTTTGACATG+AGG + chr7.2:8801266-8801285 None:intergenic 20.0%
!!! CTTTTTTTCCAAAAATGAAA+AGG + chr7.2:8799817-8799836 None:intergenic 20.0%
!!! TGATTTCGTAAAATTGATTT+TGG - chr7.2:8800836-8800855 MS.gene24642:intron 20.0%
!!! TTGAATTTTTGATACGTTAT+AGG - chr7.2:8800649-8800668 MS.gene24642:intron 20.0%
! AAATTTAAGGATTCAATACG+AGG - chr7.2:8799895-8799914 MS.gene24642:intron 25.0%
! AAGCTTTCAATGAAAAGAAT+AGG - chr7.2:8800696-8800715 MS.gene24642:intron 25.0%
! AATCACATCAACAATTTAAG+AGG + chr7.2:8801429-8801448 None:intergenic 25.0%
! AGAAATTGAAAAGAAGAAGA+TGG - chr7.2:8799646-8799665 MS.gene24642:intron 25.0%
! AGCTTTCAATGAAAAGAATA+GGG - chr7.2:8800697-8800716 MS.gene24642:intron 25.0%
! ATCAACATGCTTATGAAAAA+AGG + chr7.2:8800360-8800379 None:intergenic 25.0%
! GCTAATTACTAAATTAATGG+AGG - chr7.2:8800730-8800749 MS.gene24642:intron 25.0%
! TAAATTTGAGTGTAGAAGTT+AGG + chr7.2:8801075-8801094 None:intergenic 25.0%
! TGAGGGAAAAAAAAAAAAAC+TGG + chr7.2:8801218-8801237 None:intergenic 25.0%
!! ATTTCAGTTTTACTCAAACT+TGG - chr7.2:8799787-8799806 MS.gene24642:intron 25.0%
!! TATGTTATTTGAGGAATATC+AGG - chr7.2:8800423-8800442 MS.gene24642:intron 25.0%
!! TGCAAACTTTATGTTATTTG+AGG - chr7.2:8800414-8800433 MS.gene24642:intron 25.0%
AAATCAATTCTACTCTACCA+AGG + chr7.2:8800996-8801015 None:intergenic 30.0%
AAATTGTTGATGTGATTAGC+TGG - chr7.2:8801432-8801451 MS.gene24642:CDS 30.0%
AACAAAGAATTAAAGCCACT+TGG - chr7.2:8800514-8800533 MS.gene24642:CDS 30.0%
CAAAATATAAACCTCACGAA+TGG + chr7.2:8799735-8799754 None:intergenic 30.0%
GATCATTCACAAAAACGATT+AGG + chr7.2:8800769-8800788 None:intergenic 30.0%
GTAATATGTATTTGCTTCTG+CGG + chr7.2:8801176-8801195 None:intergenic 30.0%
GTATATTCAATGTGTGTGTT+TGG + chr7.2:8801322-8801341 None:intergenic 30.0%
TCAATTCAATCAACCTTCTT+TGG - chr7.2:8800968-8800987 MS.gene24642:intron 30.0%
TGGCTGAGGATATTATTTAA+AGG + chr7.2:8800323-8800342 None:intergenic 30.0%
! CTTTTCATTGAAAGCTTCAA+GGG + chr7.2:8800693-8800712 None:intergenic 30.0%
! TCTTTTCATTGAAAGCTTCA+AGG + chr7.2:8800694-8800713 None:intergenic 30.0%
! TTGGAAACCCTTTTCATTTT+TGG - chr7.2:8799806-8799825 MS.gene24642:intron 30.0%
!! AAATTGATTTTGACATGAGG+AGG + chr7.2:8801263-8801282 None:intergenic 30.0%
ACTTGATTATACCCGATCTA+TGG - chr7.2:8800126-8800145 MS.gene24642:intron 35.0%
AGTGAAACTTCTTTCCTCAT+TGG + chr7.2:8800469-8800488 None:intergenic 35.0%
AGTTGGTTGTACAGAATTCA+GGG - chr7.2:8799540-8799559 MS.gene24642:CDS 35.0%
ATCTTTAGGCTGATACATGT+GGG - chr7.2:8801348-8801367 MS.gene24642:CDS 35.0%
GAAAACTCTTATAATTCCCC+TGG - chr7.2:8800238-8800257 MS.gene24642:intron 35.0%
GTAGTTGTTATGTTGGAATC+TGG - chr7.2:8799920-8799939 MS.gene24642:intron 35.0%
TATCTTTAGGCTGATACATG+TGG - chr7.2:8801347-8801366 MS.gene24642:CDS 35.0%
TTCTGACAAGAACAAATCCA+GGG + chr7.2:8800258-8800277 None:intergenic 35.0%
TTTCTGACAAGAACAAATCC+AGG + chr7.2:8800259-8800278 None:intergenic 35.0%
! AATGTGTGTGTTTGGTTTCT+CGG + chr7.2:8801314-8801333 None:intergenic 35.0%
! GTCTGCTCTATCAACTTTTA+GGG - chr7.2:8799613-8799632 MS.gene24642:intron 35.0%
! TCTAGAACAGAACCATAGAT+CGG + chr7.2:8800141-8800160 None:intergenic 35.0%
AAGATTGAGGATGAAGAAGC+TGG - chr7.2:8801468-8801487 MS.gene24642:CDS 40.0%
AAGCAACAGTAACAACCAGT+TGG - chr7.2:8799523-8799542 MS.gene24642:CDS 40.0%
AGACAAGGTTCAGTTTCAGT+TGG - chr7.2:8799676-8799695 MS.gene24642:intron 40.0%
AGGAAGATGAAGAGATGTCA+AGG - chr7.2:8799591-8799610 MS.gene24642:intron 40.0%
CAGTTGGTTGTACAGAATTC+AGG - chr7.2:8799539-8799558 MS.gene24642:CDS 40.0%
CGAGGAAGTAGTTGTTATGT+TGG - chr7.2:8799913-8799932 MS.gene24642:intron 40.0%
CTAGAACAGAACCATAGATC+GGG + chr7.2:8800140-8800159 None:intergenic 40.0%
CTGAATTCTGTACAACCAAC+TGG + chr7.2:8799541-8799560 None:intergenic 40.0%
CTTTAGCAGTCACTTGTCAA+AGG - chr7.2:8799571-8799590 MS.gene24642:intron 40.0%
GAAGATGGAAGTTAGAGACA+AGG - chr7.2:8799661-8799680 MS.gene24642:intron 40.0%
GACAAGGTTCAGTTTCAGTT+GGG - chr7.2:8799677-8799696 MS.gene24642:intron 40.0%
TCACATCAGAACTACACACT+AGG + chr7.2:8800794-8800813 None:intergenic 40.0%
TCTGACAAGAACAAATCCAG+GGG + chr7.2:8800257-8800276 None:intergenic 40.0%
TCTGGTGAACAAGAGTTTGT+TGG - chr7.2:8799938-8799957 MS.gene24642:intron 40.0%
TCTTTAGGCTGATACATGTG+GGG - chr7.2:8801349-8801368 MS.gene24642:CDS 40.0%
TGTCCACTTGAGAAACATGA+GGG + chr7.2:8801235-8801254 None:intergenic 40.0%
TTGTCCACTTGAGAAACATG+AGG + chr7.2:8801236-8801255 None:intergenic 40.0%
TTTCCCTCATGTTTCTCAAG+TGG - chr7.2:8801229-8801248 MS.gene24642:intron 40.0%
TTTCCTCATTGGATCTGCAA+GGG + chr7.2:8800458-8800477 None:intergenic 40.0%
! ATCAACCTTCTTTGGTTCCT+TGG - chr7.2:8800976-8800995 MS.gene24642:intron 40.0%
! GGTCTGCTCTATCAACTTTT+AGG - chr7.2:8799612-8799631 MS.gene24642:intron 40.0%
! TGTTGATGTGATTAGCTGGT+GGG - chr7.2:8801436-8801455 MS.gene24642:CDS 40.0%
! TTGTTGATGTGATTAGCTGG+TGG - chr7.2:8801435-8801454 MS.gene24642:CDS 40.0%
ATTGAGGATGAAGAAGCTGG+AGG - chr7.2:8801471-8801490 MS.gene24642:CDS 45.0%
CATCAGAACTACACACTAGG+AGG + chr7.2:8800791-8800810 None:intergenic 45.0%
CTCTACCAAGGAACCAAAGA+AGG + chr7.2:8800984-8801003 None:intergenic 45.0%
CTTTCCTCATTGGATCTGCA+AGG + chr7.2:8800459-8800478 None:intergenic 45.0%
TAACCTAGCTCACCTTCTTC+TGG + chr7.2:8800549-8800568 None:intergenic 45.0%
TGGGGGAAAGTGAAAGATTG+AGG - chr7.2:8801455-8801474 MS.gene24642:CDS 45.0%
! GTTGATGTGATTAGCTGGTG+GGG - chr7.2:8801437-8801456 MS.gene24642:CDS 45.0%
! TTGATGTGATTAGCTGGTGG+GGG - chr7.2:8801438-8801457 MS.gene24642:CDS 45.0%
AAGCCCTTGCAGATCCAATG+AGG - chr7.2:8800452-8800471 MS.gene24642:intron 50.0%
ACGTCTTGCTACCATTCGTG+AGG - chr7.2:8799721-8799740 MS.gene24642:intron 50.0%
TGGTCATACCTGCCAGAAGA+AGG - chr7.2:8800534-8800553 MS.gene24642:CDS 50.0%
TTCTGGCAGGTATGACCAAG+TGG + chr7.2:8800532-8800551 None:intergenic 50.0%
CTAGCTCACCTTCTTCTGGC+AGG + chr7.2:8800545-8800564 None:intergenic 55.0%
CTGCCAGAAGAAGGTGAGCT+AGG - chr7.2:8800543-8800562 MS.gene24642:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8799509 8801531 8799509 ID=MS.gene24642
chr7.2 mRNA 8799509 8801531 8799509 ID=MS.gene24642.t1;Parent=MS.gene24642
chr7.2 exon 8801298 8801531 8801298 ID=MS.gene24642.t1.exon1;Parent=MS.gene24642.t1
chr7.2 CDS 8801298 8801531 8801298 ID=cds.MS.gene24642.t1;Parent=MS.gene24642.t1
chr7.2 exon 8800485 8800595 8800485 ID=MS.gene24642.t1.exon2;Parent=MS.gene24642.t1
chr7.2 CDS 8800485 8800595 8800485 ID=cds.MS.gene24642.t1;Parent=MS.gene24642.t1
chr7.2 exon 8800289 8800369 8800289 ID=MS.gene24642.t1.exon3;Parent=MS.gene24642.t1
chr7.2 CDS 8800289 8800369 8800289 ID=cds.MS.gene24642.t1;Parent=MS.gene24642.t1
chr7.2 exon 8799509 8799589 8799509 ID=MS.gene24642.t1.exon4;Parent=MS.gene24642.t1
chr7.2 CDS 8799509 8799589 8799509 ID=cds.MS.gene24642.t1;Parent=MS.gene24642.t1
Gene Sequence

>MS.gene24642

ATGAGTGAACAACAAAGCAACAGTAACAACCAGTTGGTTGTACAGAATTCAGGGAGTTTAAGCTTTAGCAGTCACTTGTCAAAGGAAGATGAAGAGATGTCAAGGTCTGCTCTATCAACTTTTAGGGCTAAAGAAGAAGAAATTGAAAAGAAGAAGATGGAAGTTAGAGACAAGGTTCAGTTTCAGTTGGGTCGTGTTGAAGAAGAAACTAAACGTCTTGCTACCATTCGTGAGGTTTATATTTTGAACCTTTTTTTTTCTTCTTAGATTTATCTTGAATTTCAGTTTTACTCAAACTTGGAAACCCTTTTCATTTTTGGAAAAAAAGTTTTTTTTTTTTTTTTAAGAAATTTTGAAAAAAGTAAATTGACCAAACATAAGTACAAAAATTTAAGGATTCAATACGAGGAAGTAGTTGTTATGTTGGAATCTGGTGAACAAGAGTTTGTTGGAATGAATTAAATTAAATTAAATTTTATTTTATTTTTTTAATTGAACTATTTATTTATTTTTGCAAAAATAACAAGTTACTTTGTCTTTTTCTTGCATGTTATTCTTAATAAATCAAGTTTTGTTGTAATAATTTGATTAATTGAGGCTTGTAGAACATGTAGAACACTTGATTATACCCGATCTATGGTTCTGTTCTAGAAACAGAGTGAATTTGCATTAAAAAATAATTATATTTTTCTTTTCTCTTGTGTTTTATGAGAAAGTAATATAAAATTTGAAAACTCTTATAATTCCCCTGGATTTGTTCTTGTCAGAAAGTAGTATTAATTAATATATGAAACAAATAATTAATAATTTGCCTTTAAATAATATCCTCAGCCATTATTTTTTTTTTTCCTTTTTTCATAAGCATGTTGATATTGATATTGATATTGATATGTAATTGAACAAATTGCAAACTTTATGTTATTTGAGGAATATCAGGAGCTTGAAGCCCTTGCAGATCCAATGAGGAAAGAAGTTTCACTTGTTAGAAAGAGAATTGATTCAGTAAACAAAGAATTAAAGCCACTTGGTCATACCTGCCAGAAGAAGGTGAGCTAGGTTATTTTTAAATTCATAGAGAAACTGTTAATTAGTGTTTTTGGTCTAATGATATTTTATTAGTGTCACCATTGAAATTTGAAATTGAATTTTTGATACGTTATAGGAGAAAGAATACAAAGATGCCCTTGAAGCTTTCAATGAAAAGAATAGGGAAAAAGTACAGCTAATTACTAAATTAATGGAGGTTAGTCTCAAATTCCTAATCGTTTTTGTGAATGATCCTCCTAGTGTGTAGTTCTGATGTGATCAAAATTGAATTTGAATGTATTGATTTCGTAAAATTGATTTTGGTTAAAAGTTAGTTGATTGTGAAATGATTTGTATGATTATTTATATTCACATCAAAGTGAGTTCACAAACAAATTTTAGTTTCAAGTTGGTATGAACTCTAAAGAAAAGATCAATTCAATCAACCTTCTTTGGTTCCTTGGTAGAGTAGAATTGATTTTAACTTTAAAATTGATTCTAACTTGAAACTATAACTTGTAGTTATTTTATTCTACACCTAACTTCTACACTCAAATTTATTGTTCAACTCACACGTGCACAAATATATGCGAATATAAATTAGTGAGAATCAATTCACTCAAAAACAATTTTTATCACCGCAGAAGCAAATACATATTACAAATGAGAATGGATTGACTCCAGTTTTTTTTTTTTTCCCTCATGTTTCTCAAGTGGACAATTTTCCTCCTCATGTCAAAATCAATTTTATGTTTATAATCATTGTTAGCTGTCTACCGAGAAACCAAACACACACATTGAATATACATAAATATCTTTAGGCTGATACATGTGGGGTTTTATGTTCTTAATTTTATTGATTGATGTTGTTGCTACTAATTATTAGTAATACCTCTTAAATTGTTGATGTGATTAGCTGGTGGGGGAAAGTGAAAGATTGAGGATGAAGAAGCTGGAGGAGCTGAGTAAGAACATAGATTCCATGCAAATGCAATGA

Protein sequence

>MS.gene24642.t1

MSEQQSNSNNQLVVQNSGSLSFSSHLSKEDEEMSRSALSTFRAKEEEIEKKKMEVRDKVQFQLGRVEEETKRLATIREELEALADPMRKEVSLVRKRIDSVNKELKPLGHTCQKKEKEYKDALEAFNEKNREKVQLITKLMELVGESERLRMKKLEELSKNIDSMQMQ