AlfalfaGEDB | MS.gene25357

M. sativa cultivar XinJiangDaYe / MS.gene25357

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25357.t1	NP_195713.1	100	136	0	0	1	136	1	136	8.90E-65	256.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25357.t1	P59169	100.0	136	0	0	1	136	1	136	2.1e-67	256.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25357.t1	A0A0V0RDG7	100.0	136	0	0	1	136	1	136	6.4e-65	256.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene25357	MS.gene017431	PPI
MS.gene25357	MS.gene70258	PPI
MS.gene25357	MS.gene056335	PPI
MS.gene25357	MS.gene58112	PPI
MS.gene25357	MS.gene011710	PPI
MS.gene60199	MS.gene25357	PPI
MS.gene44457	MS.gene25357	PPI
MS.gene25357	MS.gene23886	PPI
MS.gene25357	MS.gene015760	PPI
MS.gene25357	MS.gene89203	PPI
MS.gene44458	MS.gene25357	PPI
MS.gene66051	MS.gene25357	PPI
MS.gene25357	MS.gene63118	PPI
MS.gene25357	MS.gene068178	PPI
MS.gene25357	MS.gene027904	PPI
MS.gene030666	MS.gene25357	PPI
MS.gene25357	MS.gene059054	PPI
MS.gene25357	MS.gene60196	PPI
MS.gene25357	MS.gene34797	PPI
MS.gene049111	MS.gene25357	PPI
MS.gene25357	MS.gene64996	PPI
MS.gene25357	MS.gene26084	PPI
MS.gene70263	MS.gene25357	PPI
MS.gene25357	MS.gene78227	PPI
MS.gene25357	MS.gene65125	PPI
MS.gene011711	MS.gene25357	PPI
MS.gene25357	MS.gene29445	PPI
MS.gene25357	MS.gene78224	PPI
MS.gene25357	MS.gene038612	PPI
MS.gene61192	MS.gene25357	PPI
MS.gene25357	MS.gene030661	PPI
MS.gene25357	MS.gene70990	PPI
MS.gene25357	MS.gene24196	PPI
MS.gene25357	MS.gene28438	PPI
MS.gene25357	MS.gene70261	PPI
MS.gene25357	MS.gene009155	PPI
MS.gene78924	MS.gene25357	PPI
MS.gene58285	MS.gene25357	PPI
MS.gene25357	MS.gene91631	PPI
MS.gene25357	MS.gene58114	PPI
MS.gene027907	MS.gene25357	PPI
MS.gene25357	MS.gene04748	PPI
MS.gene58116	MS.gene25357	PPI
MS.gene030661	MS.gene25357	PPI
MS.gene25357	MS.gene05905	PPI
MS.gene25357	MS.gene64164	PPI
MS.gene28432	MS.gene25357	PPI
MS.gene25357	MS.gene057458	PPI
MS.gene25357	MS.gene26406	PPI
MS.gene25357	MS.gene068179	PPI
MS.gene25357	MS.gene027909	PPI
MS.gene021887	MS.gene25357	PPI
MS.gene25357	MS.gene97703	PPI
MS.gene39538	MS.gene25357	PPI
MS.gene25357	MS.gene64998	PPI
MS.gene25357	MS.gene00233	PPI
MS.gene78922	MS.gene25357	PPI
MS.gene071851	MS.gene25357	PPI
MS.gene25357	MS.gene030664	PPI
MS.gene25357	MS.gene47573	PPI
MS.gene28434	MS.gene25357	PPI
MS.gene25357	MS.gene60200	PPI
MS.gene25357	MS.gene61193	PPI
MS.gene97701	MS.gene25357	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25357.t1	MTR_4g097175	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene25357.t1	MTR_1g023630	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene25357.t1	MTR_8g061940	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene25357.t1	MTR_8g099430	99.265	136	1	0	1	136	1	136	4.84e-96	272
MS.gene25357.t1	MTR_4g097170	99.265	136	1	0	1	136	42	177	7.71e-95	271
MS.gene25357.t1	MTR_7g059070	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_5g029820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_5g029770	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_4g065990	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_4g088150	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_2g035230	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_8g092720	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_8g063500	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_8g103245	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_8g092820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene25357.t1	MTR_7g013610	97.059	136	4	0	1	136	100	235	2.63e-92	266
MS.gene25357.t1	MTR_2g082310	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene25357.t1	MTR_2g082340	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene25357.t1	MTR_2g082370	86.301	146	10	1	1	136	1	146	3.79e-84	243
MS.gene25357.t1	MTR_2g082400	85.294	136	18	2	1	135	1	135	1.39e-76	223
MS.gene25357.t1	MTR_7g013600	83.036	112	19	0	25	136	2	113	1.72e-62	187
MS.gene25357.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	3.79e-56	172
MS.gene25357.t1	MTR_1g023630	77.477	111	23	1	1	111	1	109	1.51e-54	167
MS.gene25357.t1	MTR_7g023880	60.150	133	39	3	3	134	18	137	2.40e-44	142
MS.gene25357.t1	MTR_5g024630	63.793	116	39	2	1	116	1	113	1.54e-42	136
MS.gene25357.t1	MTR_5g024530	65.455	110	15	2	19	128	68	154	6.25e-40	131
MS.gene25357.t1	MTR_0197s0100	96.970	66	2	0	1	66	1	66	1.84e-39	129
MS.gene25357.t1	MTR_8g027840	56.122	98	42	1	37	133	28	125	4.65e-31	108
MS.gene25357.t1	MTR_8g105150	59.783	92	3	2	37	127	11	69	1.24e-26	95.1
MS.gene25357.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	5.77e-20	80.5
MS.gene25357.t1	MTR_5g089120	68.085	47	15	0	82	128	6	52	1.58e-16	70.1
MS.gene25357.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.14e-15	66.6
MS.gene25357.t1	MTR_4g128040	72.973	37	10	0	79	115	20	56	6.10e-14	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25357.t1	AT5G10980	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene25357.t1	AT4G40030	100.000	136	0	0	1	136	29	164	2.04e-96	275
MS.gene25357.t1	AT5G65360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene25357.t1	AT5G10390	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene25357.t1	AT5G10400	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene25357.t1	AT3G27360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene25357.t1	AT1G09200	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene25357.t1	AT1G75600	96.324	136	5	0	1	136	1	136	1.02e-92	264
MS.gene25357.t1	AT1G13370	94.853	136	7	0	1	136	1	136	2.16e-91	261
MS.gene25357.t1	AT5G65350	92.647	136	10	0	1	136	1	136	1.27e-88	254
MS.gene25357.t1	AT1G19890	90.511	137	12	1	1	136	1	137	1.89e-85	246
MS.gene25357.t1	AT5G12910	71.111	135	34	2	1	135	1	130	1.15e-65	196
MS.gene25357.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene25357.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene25357.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene25357.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112

Find 38 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGCTGTTCTTGCACTTC+AGG	0.281295	8.2:-39119920	MS.gene25357:CDS
TATTCGTGGTGAACGTGCTT+AGG	0.304195	8.2:-39119791	None:intergenic
AGCAGATTTCCTTGCAGCTT+TGG	0.347069	8.2:+39120113	None:intergenic
TCACGAAGAGCGACGGTTCC+AGG	0.363931	8.2:+39120051	None:intergenic
CTTCCTTGGTGCTTTGCCGC+CGG	0.391014	8.2:+39120146	None:intergenic
GGAAGCGGCTGAGGCTTATC+TGG	0.391484	8.2:-39119899	MS.gene25357:CDS
TTCTTCACTCCGCCGGTAGT+TGG	0.407798	8.2:+39120090	None:intergenic
ATTCGTGGTGAACGTGCTTA+GGG	0.417330	8.2:-39119790	None:intergenic
TTATCTGGTGGGATTGTTTG+AGG	0.425602	8.2:-39119884	MS.gene25357:CDS
CAGCTTGCTCGTCGTATTCG+TGG	0.425806	8.2:-39119805	MS.gene25357:CDS
TGCACTTCAGGAAGCGGCTG+AGG	0.430700	8.2:-39119908	MS.gene25357:CDS
TTGGTGGCGAGTTGCTTCCT+TGG	0.434612	8.2:+39120132	None:intergenic
CCTTGGTGCTTTGCCGCCGG+TGG	0.443251	8.2:+39120149	None:intergenic
AGATTTCCTTGCAGCTTTGG+TGG	0.476244	8.2:+39120116	None:intergenic
TCTTGTTCGTGAAATTGCTC+AGG	0.482836	8.2:-39119971	MS.gene25357:CDS
CAGTACTCTTCTGATACTTA+CGG	0.507336	8.2:+39120025	None:intergenic
AAGCCTCATCGTTATCGTCC+TGG	0.515117	8.2:-39120069	MS.gene25357:CDS
AATTGCTCAGGATTTCAAAA+CGG	0.537563	8.2:-39119959	MS.gene25357:CDS
GACGCTGGAATGGAAGCTTA+CGG	0.558442	8.2:+39119992	None:intergenic
TGTTCTTGCACTTCAGGAAG+CGG	0.559874	8.2:-39119914	MS.gene25357:CDS
CGAGCAAGCTGAATATCCTT+CGG	0.565564	8.2:+39119817	None:intergenic
TCACGAACAAGACGCTGGAA+TGG	0.567002	8.2:+39119982	None:intergenic
GCGGCTGAGGCTTATCTGGT+GGG	0.567245	8.2:-39119895	MS.gene25357:CDS
CAAACTGCTCGCAAATCCAC+CGG	0.571388	8.2:-39120165	MS.gene25357:CDS
AGCGGCTGAGGCTTATCTGG+TGG	0.574844	8.2:-39119896	MS.gene25357:CDS
AGGAAATCTGCTCCAACTAC+CGG	0.580400	8.2:-39120102	MS.gene25357:CDS
AGGATTTCAAAACGGATCTG+AGG	0.592218	8.2:-39119951	MS.gene25357:CDS
ACGGATCTCACGAAGAGCGA+CGG	0.600835	8.2:+39120044	None:intergenic
ATGAGGCTTCTTCACTCCGC+CGG	0.601615	8.2:+39120083	None:intergenic
AACTCGCCACCAAAGCTGCA+AGG	0.602982	8.2:-39120122	MS.gene25357:CDS
CAATTTCACGAACAAGACGC+TGG	0.604340	8.2:+39119977	None:intergenic
GTGTGCTATTCATGCTAAGA+GGG	0.612146	8.2:-39119851	MS.gene25357:CDS
TGTGTGCTATTCATGCTAAG+AGG	0.615466	8.2:-39119852	MS.gene25357:CDS
GAGGGTGACGATTATGCCGA+AGG	0.625074	8.2:-39119833	MS.gene25357:CDS
CCACCGGCGGCAAAGCACCA+AGG	0.632902	8.2:-39120149	MS.gene25357:CDS
ACTGCTCGCAAATCCACCGG+CGG	0.667900	8.2:-39120162	MS.gene25357:CDS
AAATCTGCTCCAACTACCGG+CGG	0.697106	8.2:-39120099	MS.gene25357:CDS
GTTCCAGGACGATAACGATG+AGG	0.738178	8.2:+39120066	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTGCTCAGGATTTCAAAA+CGG	-	chr8.2:39120013-39120032	MS.gene25357:CDS	30.0%
	CAGTACTCTTCTGATACTTA+CGG	+	chr8.2:39119950-39119969	None:intergenic	35.0%
	AGGATTTCAAAACGGATCTG+AGG	-	chr8.2:39120021-39120040	MS.gene25357:CDS	40.0%
	TCTTGTTCGTGAAATTGCTC+AGG	-	chr8.2:39120001-39120020	MS.gene25357:CDS	40.0%
	TTATCTGGTGGGATTGTTTG+AGG	-	chr8.2:39120088-39120107	MS.gene25357:CDS	40.0%
!	GTGTGCTATTCATGCTAAGA+GGG	-	chr8.2:39120121-39120140	MS.gene25357:CDS	40.0%
!	TGTGTGCTATTCATGCTAAG+AGG	-	chr8.2:39120120-39120139	MS.gene25357:CDS	40.0%
	AGCAGATTTCCTTGCAGCTT+TGG	+	chr8.2:39119862-39119881	None:intergenic	45.0%
	AGGAAATCTGCTCCAACTAC+CGG	-	chr8.2:39119870-39119889	MS.gene25357:CDS	45.0%
	CAATTTCACGAACAAGACGC+TGG	+	chr8.2:39119998-39120017	None:intergenic	45.0%
	CGAGCAAGCTGAATATCCTT+CGG	+	chr8.2:39120158-39120177	None:intergenic	45.0%
	TCATGCTGTTCTTGCACTTC+AGG	-	chr8.2:39120052-39120071	MS.gene25357:CDS	45.0%
	TGTTCTTGCACTTCAGGAAG+CGG	-	chr8.2:39120058-39120077	MS.gene25357:CDS	45.0%
!	AGATTTCCTTGCAGCTTTGG+TGG	+	chr8.2:39119859-39119878	None:intergenic	45.0%
	AAATCTGCTCCAACTACCGG+CGG	-	chr8.2:39119873-39119892	MS.gene25357:CDS	50.0%
	AAGCCTCATCGTTATCGTCC+TGG	-	chr8.2:39119903-39119922	MS.gene25357:CDS	50.0%
	CAAACTGCTCGCAAATCCAC+CGG	-	chr8.2:39119807-39119826	MS.gene25357:CDS	50.0%
	GACGCTGGAATGGAAGCTTA+CGG	+	chr8.2:39119983-39120002	None:intergenic	50.0%
	GTTCCAGGACGATAACGATG+AGG	+	chr8.2:39119909-39119928	None:intergenic	50.0%
	TCACGAACAAGACGCTGGAA+TGG	+	chr8.2:39119993-39120012	None:intergenic	50.0%
	AACTCGCCACCAAAGCTGCA+AGG	-	chr8.2:39119850-39119869	MS.gene25357:CDS	55.0%
	ACGGATCTCACGAAGAGCGA+CGG	+	chr8.2:39119931-39119950	None:intergenic	55.0%
	ATGAGGCTTCTTCACTCCGC+CGG	+	chr8.2:39119892-39119911	None:intergenic	55.0%
	GAGGGTGACGATTATGCCGA+AGG	-	chr8.2:39120139-39120158	MS.gene25357:CDS	55.0%
	TTCTTCACTCCGCCGGTAGT+TGG	+	chr8.2:39119885-39119904	None:intergenic	55.0%
!	CAGCTTGCTCGTCGTATTCG+TGG	-	chr8.2:39120167-39120186	MS.gene25357:CDS	55.0%
!!	TTGGTGGCGAGTTGCTTCCT+TGG	+	chr8.2:39119843-39119862	None:intergenic	55.0%
	ACTGCTCGCAAATCCACCGG+CGG	-	chr8.2:39119810-39119829	MS.gene25357:CDS	60.0%
	AGCGGCTGAGGCTTATCTGG+TGG	-	chr8.2:39120076-39120095	MS.gene25357:CDS	60.0%
	GCGGCTGAGGCTTATCTGGT+GGG	-	chr8.2:39120077-39120096	MS.gene25357:CDS	60.0%
	GGAAGCGGCTGAGGCTTATC+TGG	-	chr8.2:39120073-39120092	MS.gene25357:CDS	60.0%
	TCACGAAGAGCGACGGTTCC+AGG	+	chr8.2:39119924-39119943	None:intergenic	60.0%
	TGCACTTCAGGAAGCGGCTG+AGG	-	chr8.2:39120064-39120083	MS.gene25357:CDS	60.0%
!!	CTTCCTTGGTGCTTTGCCGC+CGG	+	chr8.2:39119829-39119848	None:intergenic	60.0%
!	CCACCGGCGGCAAAGCACCA+AGG	-	chr8.2:39119823-39119842	MS.gene25357:CDS	70.0%
!!	CCTTGGTGCTTTGCCGCCGG+TGG	+	chr8.2:39119826-39119845	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	39119792	39120202	39119792	ID=MS.gene25357
chr8.2	mRNA	39119792	39120202	39119792	ID=MS.gene25357.t1;Parent=MS.gene25357
chr8.2	exon	39119792	39120202	39119792	ID=MS.gene25357.t1.exon1;Parent=MS.gene25357.t1
chr8.2	CDS	39119792	39120202	39119792	ID=cds.MS.gene25357.t1;Parent=MS.gene25357.t1

Gene Sequence

>MS.gene25357

ATGGCTCGTACGAAGCAAACTGCTCGCAAATCCACCGGCGGCAAAGCACCAAGGAAGCAACTCGCCACCAAAGCTGCAAGGAAATCTGCTCCAACTACCGGCGGAGTGAAGAAGCCTCATCGTTATCGTCCTGGAACCGTCGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGTACTGAGCTTTTGATCCGTAAGCTTCCATTCCAGCGTCTTGTTCGTGAAATTGCTCAGGATTTCAAAACGGATCTGAGGTTTCAGAGTCATGCTGTTCTTGCACTTCAGGAAGCGGCTGAGGCTTATCTGGTGGGATTGTTTGAGGATACTAATTTGTGTGCTATTCATGCTAAGAGGGTGACGATTATGCCGAAGGATATTCAGCTTGCTCGTCGTATTCGTGGTGAACGTGCTTAG

Protein sequence

>MS.gene25357.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA