AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44458


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44458.t1 MTR_4g097175 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44458.t1 MTR_1g023630 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44458.t1 MTR_8g061940 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44458.t1 MTR_8g099430 99.265 136 1 0 1 136 1 136 4.84e-96 272
MS.gene44458.t1 MTR_4g097170 99.265 136 1 0 1 136 42 177 7.71e-95 271
MS.gene44458.t1 MTR_7g059070 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_5g029820 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_5g029770 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_4g065990 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_4g088150 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_2g035230 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_8g092720 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_8g063500 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_8g103245 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_8g092820 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44458.t1 MTR_7g013610 97.059 136 4 0 1 136 100 235 2.63e-92 266
MS.gene44458.t1 MTR_2g082310 92.647 136 10 0 1 136 1 136 4.16e-90 257
MS.gene44458.t1 MTR_2g082340 92.647 136 10 0 1 136 1 136 4.16e-90 257
MS.gene44458.t1 MTR_2g082370 86.301 146 10 1 1 136 1 146 3.79e-84 243
MS.gene44458.t1 MTR_2g082400 85.294 136 18 2 1 135 1 135 1.39e-76 223
MS.gene44458.t1 MTR_7g013600 83.036 112 19 0 25 136 2 113 1.72e-62 187
MS.gene44458.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 3.79e-56 172
MS.gene44458.t1 MTR_1g023630 77.477 111 23 1 1 111 1 109 1.51e-54 167
MS.gene44458.t1 MTR_7g023880 60.150 133 39 3 3 134 18 137 2.40e-44 142
MS.gene44458.t1 MTR_5g024630 63.793 116 39 2 1 116 1 113 1.54e-42 136
MS.gene44458.t1 MTR_5g024530 65.455 110 15 2 19 128 68 154 6.25e-40 131
MS.gene44458.t1 MTR_0197s0100 96.970 66 2 0 1 66 1 66 1.84e-39 129
MS.gene44458.t1 MTR_8g027840 56.122 98 42 1 37 133 28 125 4.65e-31 108
MS.gene44458.t1 MTR_8g105150 59.783 92 3 2 37 127 11 69 1.24e-26 95.1
MS.gene44458.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 5.77e-20 80.5
MS.gene44458.t1 MTR_5g089120 68.085 47 15 0 82 128 6 52 1.58e-16 70.1
MS.gene44458.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.14e-15 66.6
MS.gene44458.t1 MTR_4g128040 72.973 37 10 0 79 115 20 56 6.10e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44458.t1 AT5G10980 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44458.t1 AT4G40030 100.000 136 0 0 1 136 29 164 2.04e-96 275
MS.gene44458.t1 AT5G65360 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44458.t1 AT5G10390 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44458.t1 AT5G10400 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44458.t1 AT3G27360 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44458.t1 AT1G09200 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44458.t1 AT1G75600 96.324 136 5 0 1 136 1 136 1.02e-92 264
MS.gene44458.t1 AT1G13370 94.853 136 7 0 1 136 1 136 2.16e-91 261
MS.gene44458.t1 AT5G65350 92.647 136 10 0 1 136 1 136 1.27e-88 254
MS.gene44458.t1 AT1G19890 90.511 137 12 1 1 136 1 137 1.89e-85 246
MS.gene44458.t1 AT5G12910 71.111 135 34 2 1 135 1 130 1.15e-65 196
MS.gene44458.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44458.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44458.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44458.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112

Find 45 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTGCAAGTTGCTTCCT+AGG 0.341167 8.1:+25319700 None:intergenic
CCTTGCCAACTGGATGTCCT+TGG 0.351370 8.1:+25319020 None:intergenic
CACCGAAGAGCAACAGTTCC+GGG 0.355253 8.1:+25319524 None:intergenic
CACGGATCCTCCTTGCCAAC+TGG 0.363910 8.1:+25319010 None:intergenic
TCACCGAAGAGCAACAGTTC+CGG 0.390740 8.1:+25319523 None:intergenic
ATCCGTGGTGAGCGCGCTTA+AGG 0.403617 8.1:-25318994 None:intergenic
CTTGCCAACTGGATGTCCTT+GGG 0.434839 8.1:+25319021 None:intergenic
TGAAATTGCTCAGGACTTCA+AGG 0.441698 8.1:-25319271 MS.gene44458:intron
ATATCTTGTTGGTCTGTTTG+AGG 0.444642 8.1:-25319088 MS.gene44458:CDS
GCTGCTGAGGCATATCTTGT+TGG 0.457640 8.1:-25319099 MS.gene44458:CDS
CGTAAGTCTGCACCCACTAC+TGG 0.465484 8.1:-25319575 MS.gene44458:CDS
GCCCCGGAACTGTTGCTCTT+CGG 0.468662 8.1:-25319526 MS.gene44458:intron
TGCATTGCAAGAGGCTGCTG+AGG 0.479818 8.1:-25319112 MS.gene44458:CDS
AGAGTACTGAGCTCTTGATC+AGG 0.482557 8.1:-25319320 MS.gene44458:CDS
CAAACTGCTCGTAAGTCTAC+TGG 0.493098 8.1:-25319733 MS.gene44458:CDS
TTTCTTAACTCCACCAGTAG+TGG 0.493833 8.1:+25319562 None:intergenic
ATGCAAGCACAGCATGACTC+TGG 0.500306 8.1:+25319130 None:intergenic
CAATTTCACGAACCAACCTC+TGG 0.507537 8.1:+25319286 None:intergenic
GTTGGTTCGTGAAATTGCTC+AGG 0.513873 8.1:-25319280 MS.gene44458:CDS
TGCTGTGCTTGCATTGCAAG+AGG 0.513898 8.1:-25319121 MS.gene44458:CDS
TTCTTAACTCCACCAGTAGT+GGG 0.525371 8.1:+25319563 None:intergenic
GAAGCTCCCCTTCCAGAGGT+TGG 0.527763 8.1:-25319298 MS.gene44458:CDS
AGACTTACGAGCAGTTTGCT+TGG 0.530724 8.1:+25319738 None:intergenic
TAATAGTAACTCGCTTGGCA+TGG 0.547452 8.1:+25319046 None:intergenic
CACGAACCAACCTCTGGAAG+GGG 0.550311 8.1:+25319292 None:intergenic
GGGCATAATAGTAACTCGCT+TGG 0.573260 8.1:+25319041 None:intergenic
AAACCACATCGTTACCGCCC+CGG 0.580189 8.1:-25319542 MS.gene44458:CDS
TCACGAACCAACCTCTGGAA+GGG 0.587957 8.1:+25319291 None:intergenic
CCAAGGACATCCAGTTGGCA+AGG 0.588868 8.1:-25319020 MS.gene44458:CDS
CTCCTTAAGCGCGCTCACCA+CGG 0.591808 8.1:+25318992 None:intergenic
TTCACGAACCAACCTCTGGA+AGG 0.592307 8.1:+25319290 None:intergenic
TCGTAAGTCTACTGGTGGAA+AGG 0.600087 8.1:-25319725 MS.gene44458:CDS
TAGGAAGCAACTTGCAACCA+AGG 0.610266 8.1:-25319698 MS.gene44458:intron
AAGTCTGCACCCACTACTGG+TGG 0.628549 8.1:-25319572 MS.gene44458:CDS
GCGAGTTACTATTATGCCCA+AGG 0.629580 8.1:-25319037 MS.gene44458:CDS
TCAGGAAGCTCCCCTTCCAG+AGG 0.635098 8.1:-25319302 MS.gene44458:CDS
CTACTGGTGGAAAGGCACCT+AGG 0.637308 8.1:-25319717 MS.gene44458:CDS
ACCGAAGAGCAACAGTTCCG+GGG 0.639513 8.1:+25319525 None:intergenic
GTTCCGGGGCGGTAACGATG+TGG 0.646981 8.1:+25319539 None:intergenic
TATGCCCAAGGACATCCAGT+TGG 0.656475 8.1:-25319025 MS.gene44458:CDS
ACTGCTCGTAAGTCTACTGG+TGG 0.660657 8.1:-25319730 MS.gene44458:CDS
CAGTTGGCAAGGAGGATCCG+TGG 0.664833 8.1:-25319009 MS.gene44458:CDS
CAGCATGACTCTGGAAACGA+AGG 0.672290 8.1:+25319139 None:intergenic
AGGACATCCAGTTGGCAAGG+AGG 0.674393 8.1:-25319017 MS.gene44458:CDS
GAAGAGCAACAGTTCCGGGG+CGG 0.724004 8.1:+25319528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATGAAATGATTGTATT+GGG - chr8.1:25319292-25319311 MS.gene44458:CDS 15.0%
!! TTTATATGAAATGATTGTAT+TGG - chr8.1:25319291-25319310 MS.gene44458:CDS 15.0%
!! ATCATCATATTCATAATCAT+TGG - chr8.1:25319080-25319099 MS.gene44458:CDS 20.0%
!!! ATCAGTTAGATAATAAGTTT+TGG - chr8.1:25319541-25319560 MS.gene44458:CDS 20.0%
!!! ATTCTGTTGAAATTTTCTTT+AGG - chr8.1:25319245-25319264 MS.gene44458:intron 20.0%
!!! TTCTGTTGAAATTTTCTTTA+GGG - chr8.1:25319246-25319265 MS.gene44458:intron 20.0%
!!! TTGTTTGAAGTAATTGTATT+GGG - chr8.1:25319338-25319357 MS.gene44458:CDS 20.0%
!!! TTTGTTTGAAGTAATTGTAT+TGG - chr8.1:25319337-25319356 MS.gene44458:CDS 20.0%
! CTAACTGATTATGAAAACAA+AGG + chr8.1:25319530-25319549 None:intergenic 25.0%
!!! TATCTGTTTTTGTTTCCTTT+AGG - chr8.1:25319141-25319160 MS.gene44458:CDS 25.0%
!!! TTGGTTGTTTCTGATAATTT+GGG - chr8.1:25319560-25319579 MS.gene44458:CDS 25.0%
!!! TTGTTTTAAACAGTGAGATT+AGG - chr8.1:25319391-25319410 MS.gene44458:intron 25.0%
!!! TTTGGTTGTTTCTGATAATT+TGG - chr8.1:25319559-25319578 MS.gene44458:CDS 25.0%
GATGATTCAAAAACTCACCT+TGG + chr8.1:25319066-25319085 None:intergenic 35.0%
! ATATCTTGTTGGTCTGTTTG+AGG - chr8.1:25319656-25319675 MS.gene44458:intron 35.0%
TAATAGTAACTCGCTTGGCA+TGG + chr8.1:25319701-25319720 None:intergenic 40.0%
TGAAATTGCTCAGGACTTCA+AGG - chr8.1:25319473-25319492 MS.gene44458:intron 40.0%
TTCTTAACTCCACCAGTAGT+GGG + chr8.1:25319184-25319203 None:intergenic 40.0%
TTTCTTAACTCCACCAGTAG+TGG + chr8.1:25319185-25319204 None:intergenic 40.0%
! GGTAATGTGTAGTTGCTGTT+AGG - chr8.1:25319494-25319513 MS.gene44458:intron 40.0%
CAAACTGCTCGTAAGTCTAC+TGG - chr8.1:25319011-25319030 MS.gene44458:CDS 45.0%
CAATTTCACGAACCAACCTC+TGG + chr8.1:25319461-25319480 None:intergenic 45.0%
GCGAGTTACTATTATGCCCA+AGG - chr8.1:25319707-25319726 MS.gene44458:CDS 45.0%
GGGCATAATAGTAACTCGCT+TGG + chr8.1:25319706-25319725 None:intergenic 45.0%
TAGGAAGCAACTTGCAACCA+AGG - chr8.1:25319046-25319065 MS.gene44458:CDS 45.0%
TCGTAAGTCTACTGGTGGAA+AGG - chr8.1:25319019-25319038 MS.gene44458:CDS 45.0%
TTGGTTGCAAGTTGCTTCCT+AGG + chr8.1:25319047-25319066 None:intergenic 45.0%
! AGACTTACGAGCAGTTTGCT+TGG + chr8.1:25319009-25319028 None:intergenic 45.0%
!! AGAGTACTGAGCTCTTGATC+AGG - chr8.1:25319424-25319443 MS.gene44458:intron 45.0%
!! GTTGGTTCGTGAAATTGCTC+AGG - chr8.1:25319464-25319483 MS.gene44458:intron 45.0%
ACTGCTCGTAAGTCTACTGG+TGG - chr8.1:25319014-25319033 MS.gene44458:CDS 50.0%
ATGCAAGCACAGCATGACTC+TGG + chr8.1:25319617-25319636 None:intergenic 50.0%
CAGACTTACGTGCAGCCTAA+AGG + chr8.1:25319159-25319178 None:intergenic 50.0%
CTTGCCAACTGGATGTCCTT+GGG + chr8.1:25319726-25319745 None:intergenic 50.0%
GCTGCTGAGGCATATCTTGT+TGG - chr8.1:25319645-25319664 MS.gene44458:intron 50.0%
TATGCCCAAGGACATCCAGT+TGG - chr8.1:25319719-25319738 MS.gene44458:CDS 50.0%
TCACCGAAGAGCAACAGTTC+CGG + chr8.1:25319224-25319243 None:intergenic 50.0%
TGCTGTGCTTGCATTGCAAG+AGG - chr8.1:25319623-25319642 MS.gene44458:intron 50.0%
TTCACGAACCAACCTCTGGA+AGG + chr8.1:25319457-25319476 None:intergenic 50.0%
! CAGCATGACTCTGGAAACGA+AGG + chr8.1:25319608-25319627 None:intergenic 50.0%
! TCACGAACCAACCTCTGGAA+GGG + chr8.1:25319456-25319475 None:intergenic 50.0%
AAACCACATCGTTACCGCCC+CGG - chr8.1:25319202-25319221 MS.gene44458:intron 55.0%
AAGTCTGCACCCACTACTGG+TGG - chr8.1:25319172-25319191 MS.gene44458:intron 55.0%
ACCGAAGAGCAACAGTTCCG+GGG + chr8.1:25319222-25319241 None:intergenic 55.0%
AGGACATCCAGTTGGCAAGG+AGG - chr8.1:25319727-25319746 MS.gene44458:CDS 55.0%
CACCGAAGAGCAACAGTTCC+GGG + chr8.1:25319223-25319242 None:intergenic 55.0%
CCAAGGACATCCAGTTGGCA+AGG - chr8.1:25319724-25319743 MS.gene44458:CDS 55.0%
CCTTGCCAACTGGATGTCCT+TGG + chr8.1:25319727-25319746 None:intergenic 55.0%
CGTAAGTCTGCACCCACTAC+TGG - chr8.1:25319169-25319188 MS.gene44458:intron 55.0%
TGCATTGCAAGAGGCTGCTG+AGG - chr8.1:25319632-25319651 MS.gene44458:intron 55.0%
! CACGAACCAACCTCTGGAAG+GGG + chr8.1:25319455-25319474 None:intergenic 55.0%
!! CTACTGGTGGAAAGGCACCT+AGG - chr8.1:25319027-25319046 MS.gene44458:CDS 55.0%
CACGGATCCTCCTTGCCAAC+TGG + chr8.1:25319737-25319756 None:intergenic 60.0%
CAGTTGGCAAGGAGGATCCG+TGG - chr8.1:25319735-25319754 MS.gene44458:CDS 60.0%
GAAGAGCAACAGTTCCGGGG+CGG + chr8.1:25319219-25319238 None:intergenic 60.0%
GAAGCTCCCCTTCCAGAGGT+TGG - chr8.1:25319446-25319465 MS.gene44458:intron 60.0%
GCCCCGGAACTGTTGCTCTT+CGG - chr8.1:25319218-25319237 MS.gene44458:intron 60.0%
TCAGGAAGCTCCCCTTCCAG+AGG - chr8.1:25319442-25319461 MS.gene44458:intron 60.0%
GTTCCGGGGCGGTAACGATG+TGG + chr8.1:25319208-25319227 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25318996 25319770 25318996 ID=MS.gene44458
chr8.1 mRNA 25318996 25319770 25318996 ID=MS.gene44458.t1;Parent=MS.gene44458
chr8.1 exon 25319699 25319770 25319699 ID=MS.gene44458.t1.exon1;Parent=MS.gene44458.t1
chr8.1 CDS 25319699 25319770 25319699 ID=cds.MS.gene44458.t1;Parent=MS.gene44458.t1
chr8.1 exon 25319527 25319603 25319527 ID=MS.gene44458.t1.exon2;Parent=MS.gene44458.t1
chr8.1 CDS 25319527 25319603 25319527 ID=cds.MS.gene44458.t1;Parent=MS.gene44458.t1
chr8.1 exon 25319272 25319362 25319272 ID=MS.gene44458.t1.exon3;Parent=MS.gene44458.t1
chr8.1 CDS 25319272 25319362 25319272 ID=cds.MS.gene44458.t1;Parent=MS.gene44458.t1
chr8.1 exon 25318996 25319166 25318996 ID=MS.gene44458.t1.exon4;Parent=MS.gene44458.t1
chr8.1 CDS 25318996 25319166 25318996 ID=cds.MS.gene44458.t1;Parent=MS.gene44458.t1
Gene Sequence

>MS.gene44458

ATGGCACGTACCAAGCAAACTGCTCGTAAGTCTACTGGTGGAAAGGCACCTAGGAAGCAACTTGCAACCAAGGTGAGTTTTTGAATCATCATATTCATAATCATTGGTTGTTGTTTGTATTATGATGATTGAATATTTGTTTAATTATCTGTTTTTGTTTCCTTTAGGCTGCACGTAAGTCTGCACCCACTACTGGTGGAGTTAAGAAACCACATCGTTACCGCCCCGGAACTGTTGCTCTTCGGTGAGATTCTGTTGAAATTTTCTTTAGGGTTATGATTTAATGTGTTCTGTTTTTATATGAAATGATTGTATTGGGTTATGATTCAATGTGTTCTGTTTTTGTTTGAAGTAATTGTATTGGGTTCTGTTTCTAATAGCTGTTATATGAATTGTTGTTTTAAACAGTGAGATTAGGAAGTATCAGAAGAGTACTGAGCTCTTGATCAGGAAGCTCCCCTTCCAGAGGTTGGTTCGTGAAATTGCTCAGGACTTCAAGGTAATGTGTAGTTGCTGTTAGGAGAACTTGTGCCTTTGTTTTCATAATCAGTTAGATAATAAGTTTTGGTTGTTTCTGATAATTTGGGTCTTGTTGTTTTTGCAGACTGACCTTCGTTTCCAGAGTCATGCTGTGCTTGCATTGCAAGAGGCTGCTGAGGCATATCTTGTTGGTCTGTTTGAGGACACTAACTTGTGCGCTATCCATGCCAAGCGAGTTACTATTATGCCCAAGGACATCCAGTTGGCAAGGAGGATCCGTGGTGAGCGCGCTTAA

Protein sequence

>MS.gene44458.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA