AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44457


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44457.t1 MTR_4g097175 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44457.t1 MTR_1g023630 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44457.t1 MTR_8g061940 100.000 136 0 0 1 136 1 136 1.33e-96 274
MS.gene44457.t1 MTR_8g099430 99.265 136 1 0 1 136 1 136 4.84e-96 272
MS.gene44457.t1 MTR_4g097170 99.265 136 1 0 1 136 42 177 7.71e-95 271
MS.gene44457.t1 MTR_7g059070 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_5g029820 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_5g029770 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_4g065990 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_4g088150 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_2g035230 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_8g092720 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_8g063500 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_8g103245 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_8g092820 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene44457.t1 MTR_7g013610 97.059 136 4 0 1 136 100 235 2.63e-92 266
MS.gene44457.t1 MTR_2g082310 92.647 136 10 0 1 136 1 136 4.16e-90 257
MS.gene44457.t1 MTR_2g082340 92.647 136 10 0 1 136 1 136 4.16e-90 257
MS.gene44457.t1 MTR_2g082370 86.301 146 10 1 1 136 1 146 3.79e-84 243
MS.gene44457.t1 MTR_2g082400 85.294 136 18 2 1 135 1 135 1.39e-76 223
MS.gene44457.t1 MTR_7g013600 83.036 112 19 0 25 136 2 113 1.72e-62 187
MS.gene44457.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 3.79e-56 172
MS.gene44457.t1 MTR_1g023630 77.477 111 23 1 1 111 1 109 1.51e-54 167
MS.gene44457.t1 MTR_7g023880 60.150 133 39 3 3 134 18 137 2.40e-44 142
MS.gene44457.t1 MTR_5g024630 63.793 116 39 2 1 116 1 113 1.54e-42 136
MS.gene44457.t1 MTR_5g024530 65.455 110 15 2 19 128 68 154 6.25e-40 131
MS.gene44457.t1 MTR_0197s0100 96.970 66 2 0 1 66 1 66 1.84e-39 129
MS.gene44457.t1 MTR_8g027840 56.122 98 42 1 37 133 28 125 4.65e-31 108
MS.gene44457.t1 MTR_8g105150 59.783 92 3 2 37 127 11 69 1.24e-26 95.1
MS.gene44457.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 5.77e-20 80.5
MS.gene44457.t1 MTR_5g089120 68.085 47 15 0 82 128 6 52 1.58e-16 70.1
MS.gene44457.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.14e-15 66.6
MS.gene44457.t1 MTR_4g128040 72.973 37 10 0 79 115 20 56 6.10e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44457.t1 AT5G10980 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40040 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40030 100.000 136 0 0 1 136 1 136 1.39e-96 274
MS.gene44457.t1 AT4G40030 100.000 136 0 0 1 136 29 164 2.04e-96 275
MS.gene44457.t1 AT5G65360 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44457.t1 AT5G10390 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44457.t1 AT5G10400 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44457.t1 AT3G27360 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44457.t1 AT1G09200 97.059 136 4 0 1 136 1 136 3.84e-93 265
MS.gene44457.t1 AT1G75600 96.324 136 5 0 1 136 1 136 1.02e-92 264
MS.gene44457.t1 AT1G13370 94.853 136 7 0 1 136 1 136 2.16e-91 261
MS.gene44457.t1 AT5G65350 92.647 136 10 0 1 136 1 136 1.27e-88 254
MS.gene44457.t1 AT1G19890 90.511 137 12 1 1 136 1 137 1.89e-85 246
MS.gene44457.t1 AT5G12910 71.111 135 34 2 1 135 1 130 1.15e-65 196
MS.gene44457.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44457.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44457.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112
MS.gene44457.t1 AT1G01370 54.867 113 48 2 24 134 63 174 3.24e-32 112

Find 44 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTGCAAGTTGCTTCCT+TGG 0.333909 8.1:+25315885 None:intergenic
CACCGAAGAGCAACAGTTCC+TGG 0.341134 8.1:+25315698 None:intergenic
TCAAGAGCTCGGTACTCTTC+TGG 0.348030 8.1:+25315521 None:intergenic
CCTTGCCAACTGGATGTCCT+TGG 0.351370 8.1:+25315229 None:intergenic
CACGGATCCTCCTTGCCAAC+TGG 0.363910 8.1:+25315219 None:intergenic
CTTGCCAACTGGATGTCCTT+GGG 0.434839 8.1:+25315230 None:intergenic
ATATCTTGTTGGTCTCTTTG+AGG 0.438404 8.1:-25315297 MS.gene44457:CDS
TGAAATTGCTCAGGACTTCA+AGG 0.441698 8.1:-25315467 MS.gene44457:intron
CTTCCTTGGTGCCTTTCCAC+CGG 0.443477 8.1:+25315899 None:intergenic
TTCTTAACTCCACCAGTAGT+TGG 0.444620 8.1:+25315737 None:intergenic
GCTGCTGAGGCATATCTTGT+TGG 0.457640 8.1:-25315308 MS.gene44457:CDS
CACGAACCAACCTCTGGAAT+GGG 0.467959 8.1:+25315488 None:intergenic
CGCAAGTCTGCACCAACTAC+TGG 0.471576 8.1:-25315749 MS.gene44457:CDS
GCCCAGGAACTGTTGCTCTT+CGG 0.472314 8.1:-25315700 MS.gene44457:intron
TGCATTGCAAGAGGCTGCTG+AGG 0.479818 8.1:-25315321 MS.gene44457:CDS
AGAGTACCGAGCTCTTGATC+AGG 0.484025 8.1:-25315516 MS.gene44457:CDS
ATCCGTGGTGAGCGCGCCTA+AGG 0.494321 8.1:-25315203 None:intergenic
ATGCAAGCACAGCATGACTC+TGG 0.500306 8.1:+25315339 None:intergenic
CAATTTCACGAACCAACCTC+TGG 0.503886 8.1:+25315482 None:intergenic
TGACTTACGAGCTGTTTGCT+TGG 0.508768 8.1:+25315923 None:intergenic
GTTGGTTCGTGAAATTGCTC+AGG 0.513873 8.1:-25315476 MS.gene44457:CDS
TGCTGTGCTTGCATTGCAAG+AGG 0.513898 8.1:-25315330 MS.gene44457:CDS
CAAACAGCTCGTAAGTCAAC+CGG 0.519692 8.1:-25315918 MS.gene44457:CDS
TCACGAACCAACCTCTGGAA+TGG 0.521454 8.1:+25315487 None:intergenic
TAATAGTAACTCGCTTGGCA+TGG 0.547452 8.1:+25315255 None:intergenic
CTCCTTAGGCGCGCTCACCA+CGG 0.549887 8.1:+25315201 None:intergenic
TCGTAAGTCAACCGGTGGAA+AGG 0.554969 8.1:-25315910 MS.gene44457:CDS
ACCGAAGAGCAACAGTTCCT+GGG 0.559961 8.1:+25315699 None:intergenic
GAAGCTCCCATTCCAGAGGT+TGG 0.572362 8.1:-25315494 MS.gene44457:CDS
GGGCATAATAGTAACTCGCT+TGG 0.573260 8.1:+25315250 None:intergenic
AAGGAAGCAACTTGCAACCA+AGG 0.574800 8.1:-25315883 MS.gene44457:intron
AAGCCACATCGTTATCGCCC+AGG 0.581263 8.1:-25315716 MS.gene44457:CDS
CCAAGGACATCCAGTTGGCA+AGG 0.588868 8.1:-25315229 MS.gene44457:CDS
GAGCTTCCTGATCAAGAGCT+CGG 0.624511 8.1:+25315510 None:intergenic
GCGAGTTACTATTATGCCCA+AGG 0.629580 8.1:-25315246 MS.gene44457:CDS
AAGTCTGCACCAACTACTGG+TGG 0.650548 8.1:-25315746 MS.gene44457:CDS
TATGCCCAAGGACATCCAGT+TGG 0.656475 8.1:-25315234 MS.gene44457:CDS
TCAGGAAGCTCCCATTCCAG+AGG 0.664518 8.1:-25315498 MS.gene44457:CDS
CAGTTGGCAAGGAGGATCCG+TGG 0.664833 8.1:-25315218 MS.gene44457:CDS
CAGCATGACTCTGGAAACGA+AGG 0.672290 8.1:+25315348 None:intergenic
ACAGCTCGTAAGTCAACCGG+TGG 0.672375 8.1:-25315915 MS.gene44457:CDS
AGGACATCCAGTTGGCAAGG+AGG 0.674393 8.1:-25315226 MS.gene44457:CDS
CAACCGGTGGAAAGGCACCA+AGG 0.683753 8.1:-25315902 MS.gene44457:CDS
GTTCCTGGGCGATAACGATG+TGG 0.684844 8.1:+25315713 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTCTAATAATTTGTTATT+TGG - chr8.1:25315561-25315580 MS.gene44457:intron 15.0%
!!! TAATAATTTGTTATTTGGAT+TGG - chr8.1:25315566-25315585 MS.gene44457:intron 15.0%
!! TCTATTGAATTGAATTGAAT+TGG - chr8.1:25315534-25315553 MS.gene44457:CDS 20.0%
!!! TATTTTAAAACAGTGAGATT+AGG - chr8.1:25315589-25315608 MS.gene44457:intron 20.0%
!!! TTTTTTTTTTCCTTACCTTT+AGG - chr8.1:25315361-25315380 MS.gene44457:intron 20.0%
! TGAATTGAATTGAATTGGTT+TGG - chr8.1:25315539-25315558 MS.gene44457:CDS 25.0%
!! CTGTTGAAAATTTTCTTTCA+GGG - chr8.1:25315468-25315487 MS.gene44457:CDS 25.0%
!! TCTGTTGAAAATTTTCTTTC+AGG - chr8.1:25315467-25315486 MS.gene44457:intron 25.0%
AAGCAACTAAAATCAAACCT+TGG + chr8.1:25315275-25315294 None:intergenic 30.0%
AGGTAATGTGTAATTACTGT+TGG - chr8.1:25315691-25315710 MS.gene44457:intron 30.0%
GGTTGTGGTTTCTAATAATT+TGG - chr8.1:25315750-25315769 MS.gene44457:CDS 30.0%
! ATATCTTGTTGGTCTCTTTG+AGG - chr8.1:25315841-25315860 MS.gene44457:intron 35.0%
GTTCATGATCAGTGTAGTCA+AGG - chr8.1:25315729-25315748 MS.gene44457:CDS 40.0%
TAATAGTAACTCGCTTGGCA+TGG + chr8.1:25315886-25315905 None:intergenic 40.0%
TGAAATTGCTCAGGACTTCA+AGG - chr8.1:25315671-25315690 MS.gene44457:intron 40.0%
TTCTTAACTCCACCAGTAGT+TGG + chr8.1:25315404-25315423 None:intergenic 40.0%
AAGGAAGCAACTTGCAACCA+AGG - chr8.1:25315255-25315274 MS.gene44457:CDS 45.0%
CAAACAGCTCGTAAGTCAAC+CGG - chr8.1:25315220-25315239 MS.gene44457:CDS 45.0%
CAATTTCACGAACCAACCTC+TGG + chr8.1:25315659-25315678 None:intergenic 45.0%
GATCAGTGTAGTCAAGGTTG+TGG - chr8.1:25315735-25315754 MS.gene44457:CDS 45.0%
GCGAGTTACTATTATGCCCA+AGG - chr8.1:25315892-25315911 MS.gene44457:CDS 45.0%
GGGCATAATAGTAACTCGCT+TGG + chr8.1:25315891-25315910 None:intergenic 45.0%
TGACTTACGAGCTGTTTGCT+TGG + chr8.1:25315218-25315237 None:intergenic 45.0%
TTGGTTGCAAGTTGCTTCCT+TGG + chr8.1:25315256-25315275 None:intergenic 45.0%
!! GTTGGTTCGTGAAATTGCTC+AGG - chr8.1:25315662-25315681 MS.gene44457:intron 45.0%
AAGTCTGCACCAACTACTGG+TGG - chr8.1:25315392-25315411 MS.gene44457:intron 50.0%
ACCGAAGAGCAACAGTTCCT+GGG + chr8.1:25315442-25315461 None:intergenic 50.0%
ATGCAAGCACAGCATGACTC+TGG + chr8.1:25315802-25315821 None:intergenic 50.0%
CTTGCCAACTGGATGTCCTT+GGG + chr8.1:25315911-25315930 None:intergenic 50.0%
GAGCTTCCTGATCAAGAGCT+CGG + chr8.1:25315631-25315650 None:intergenic 50.0%
GCTGCTGAGGCATATCTTGT+TGG - chr8.1:25315830-25315849 MS.gene44457:intron 50.0%
TATGCCCAAGGACATCCAGT+TGG - chr8.1:25315904-25315923 MS.gene44457:CDS 50.0%
TCAAGAGCTCGGTACTCTTC+TGG + chr8.1:25315620-25315639 None:intergenic 50.0%
TCGTAAGTCAACCGGTGGAA+AGG - chr8.1:25315228-25315247 MS.gene44457:CDS 50.0%
TGCTGTGCTTGCATTGCAAG+AGG - chr8.1:25315808-25315827 MS.gene44457:intron 50.0%
TTGCGTGCAGCCTAAAGGTA+AGG + chr8.1:25315374-25315393 None:intergenic 50.0%
! CAGCATGACTCTGGAAACGA+AGG + chr8.1:25315793-25315812 None:intergenic 50.0%
! TCACGAACCAACCTCTGGAA+TGG + chr8.1:25315654-25315673 None:intergenic 50.0%
!! AGAGTACCGAGCTCTTGATC+AGG - chr8.1:25315622-25315641 MS.gene44457:intron 50.0%
!! CACGAACCAACCTCTGGAAT+GGG + chr8.1:25315653-25315672 None:intergenic 50.0%
AAGCCACATCGTTATCGCCC+AGG - chr8.1:25315422-25315441 MS.gene44457:intron 55.0%
ACAGCTCGTAAGTCAACCGG+TGG - chr8.1:25315223-25315242 MS.gene44457:CDS 55.0%
AGGACATCCAGTTGGCAAGG+AGG - chr8.1:25315912-25315931 MS.gene44457:CDS 55.0%
CACCGAAGAGCAACAGTTCC+TGG + chr8.1:25315443-25315462 None:intergenic 55.0%
CAGACTTGCGTGCAGCCTAA+AGG + chr8.1:25315379-25315398 None:intergenic 55.0%
CCAAGGACATCCAGTTGGCA+AGG - chr8.1:25315909-25315928 MS.gene44457:CDS 55.0%
CCTTGCCAACTGGATGTCCT+TGG + chr8.1:25315912-25315931 None:intergenic 55.0%
CGCAAGTCTGCACCAACTAC+TGG - chr8.1:25315389-25315408 MS.gene44457:intron 55.0%
GAAGCTCCCATTCCAGAGGT+TGG - chr8.1:25315644-25315663 MS.gene44457:intron 55.0%
GCCCAGGAACTGTTGCTCTT+CGG - chr8.1:25315438-25315457 MS.gene44457:intron 55.0%
GTTCCTGGGCGATAACGATG+TGG + chr8.1:25315428-25315447 None:intergenic 55.0%
TCAGGAAGCTCCCATTCCAG+AGG - chr8.1:25315640-25315659 MS.gene44457:intron 55.0%
TGCATTGCAAGAGGCTGCTG+AGG - chr8.1:25315817-25315836 MS.gene44457:intron 55.0%
!! CTTCCTTGGTGCCTTTCCAC+CGG + chr8.1:25315242-25315261 None:intergenic 55.0%
CACGGATCCTCCTTGCCAAC+TGG + chr8.1:25315922-25315941 None:intergenic 60.0%
CAGTTGGCAAGGAGGATCCG+TGG - chr8.1:25315920-25315939 MS.gene44457:CDS 60.0%
!! CAACCGGTGGAAAGGCACCA+AGG - chr8.1:25315236-25315255 MS.gene44457:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25315205 25315955 25315205 ID=MS.gene44457
chr8.1 mRNA 25315205 25315955 25315205 ID=MS.gene44457.t1;Parent=MS.gene44457
chr8.1 exon 25315884 25315955 25315884 ID=MS.gene44457.t1.exon1;Parent=MS.gene44457.t1
chr8.1 CDS 25315884 25315955 25315884 ID=cds.MS.gene44457.t1;Parent=MS.gene44457.t1
chr8.1 exon 25315701 25315777 25315701 ID=MS.gene44457.t1.exon2;Parent=MS.gene44457.t1
chr8.1 CDS 25315701 25315777 25315701 ID=cds.MS.gene44457.t1;Parent=MS.gene44457.t1
chr8.1 exon 25315468 25315558 25315468 ID=MS.gene44457.t1.exon3;Parent=MS.gene44457.t1
chr8.1 CDS 25315468 25315558 25315468 ID=cds.MS.gene44457.t1;Parent=MS.gene44457.t1
chr8.1 exon 25315205 25315375 25315205 ID=MS.gene44457.t1.exon4;Parent=MS.gene44457.t1
chr8.1 CDS 25315205 25315375 25315205 ID=cds.MS.gene44457.t1;Parent=MS.gene44457.t1
Gene Sequence

>MS.gene44457

ATGGCACGTACCAAGCAAACAGCTCGTAAGTCAACCGGTGGAAAGGCACCAAGGAAGCAACTTGCAACCAAGGTTTGATTTTAGTTGCTTATCATTATAAACACATTGATTTTAATCTGTATTATGACTGTTGAATCTTATTTTAACGATTTCTTGTTTTTTTTTTCCTTACCTTTAGGCTGCACGCAAGTCTGCACCAACTACTGGTGGAGTTAAGAAGCCACATCGTTATCGCCCAGGAACTGTTGCTCTTCGGTGAGTTTCTGTTGAAAATTTTCTTTCAGGGTTGTAATTCAATGTGTTCTGTTCTGTTTTGATTTTAATTTGTTTCTATTGAATTGAATTGAATTGGTTTGGTTTCTAATAATTTGTTATTTGGATTGGTATTTTAAAACAGTGAGATTAGGAAGTACCAGAAGAGTACCGAGCTCTTGATCAGGAAGCTCCCATTCCAGAGGTTGGTTCGTGAAATTGCTCAGGACTTCAAGGTAATGTGTAATTACTGTTGGATATTGTGTTTCTGTGTTCATGATCAGTGTAGTCAAGGTTGTGGTTTCTAATAATTTGGTATTTGTCGCAGACTGACCTTCGTTTCCAGAGTCATGCTGTGCTTGCATTGCAAGAGGCTGCTGAGGCATATCTTGTTGGTCTCTTTGAGGACACTAACTTGTGCGCTATCCATGCCAAGCGAGTTACTATTATGCCCAAGGACATCCAGTTGGCAAGGAGGATCCGTGGTGAGCGCGCCTAA

Protein sequence

>MS.gene44457.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA