AlfalfaGEDB | MS.gene64998

M. sativa cultivar XinJiangDaYe / MS.gene64998

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64998.t1	NP_195713.1	100	136	0	0	1	136	1	136	8.90E-65	256.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64998.t1	P59169	100.0	136	0	0	1	136	1	136	2.1e-67	256.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64998.t1	A0A0V0RDG7	100.0	136	0	0	1	136	1	136	6.4e-65	256.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene64998	MS.gene038612	PPI
MS.gene059054	MS.gene64998	PPI
MS.gene64998	MS.gene60196	PPI
MS.gene60199	MS.gene64998	PPI
MS.gene64998	MS.gene34797	PPI
MS.gene021887	MS.gene64998	PPI
MS.gene64998	MS.gene64996	PPI
MS.gene44458	MS.gene64998	PPI
MS.gene64998	MS.gene26084	PPI
MS.gene64998	MS.gene78227	PPI
MS.gene030666	MS.gene64998	PPI
MS.gene97701	MS.gene64998	PPI
MS.gene057458	MS.gene64998	PPI
MS.gene64998	MS.gene78224	PPI
MS.gene049111	MS.gene64998	PPI
MS.gene027904	MS.gene64998	PPI
MS.gene00233	MS.gene64998	PPI
MS.gene068178	MS.gene64998	PPI
MS.gene44457	MS.gene64998	PPI
MS.gene64998	MS.gene70990	PPI
MS.gene64998	MS.gene24196	PPI
MS.gene64998	MS.gene28438	PPI
MS.gene64998	MS.gene70261	PPI
MS.gene66051	MS.gene64998	PPI
MS.gene011711	MS.gene64998	PPI
MS.gene64998	MS.gene91631	PPI
MS.gene64998	MS.gene58114	PPI
MS.gene29445	MS.gene64998	PPI
MS.gene64998	MS.gene04748	PPI
MS.gene60200	MS.gene64998	PPI
MS.gene015760	MS.gene64998	PPI
MS.gene64998	MS.gene05905	PPI
MS.gene70263	MS.gene64998	PPI
MS.gene64998	MS.gene64164	PPI
MS.gene58285	MS.gene64998	PPI
MS.gene64998	MS.gene057458	PPI
MS.gene64998	MS.gene26406	PPI
MS.gene038612	MS.gene64998	PPI
MS.gene64998	MS.gene068179	PPI
MS.gene027907	MS.gene64998	PPI
MS.gene64998	MS.gene027909	PPI
MS.gene58116	MS.gene64998	PPI
MS.gene64998	MS.gene00233	PPI
MS.gene030661	MS.gene64998	PPI
MS.gene61192	MS.gene64998	PPI
MS.gene64998	MS.gene030664	PPI
MS.gene28432	MS.gene64998	PPI
MS.gene64998	MS.gene47573	PPI
MS.gene068179	MS.gene64998	PPI
MS.gene97703	MS.gene64998	PPI
MS.gene64998	MS.gene61193	PPI
MS.gene91631	MS.gene64998	PPI
MS.gene009155	MS.gene64998	PPI
MS.gene78924	MS.gene64998	PPI
MS.gene25357	MS.gene64998	PPI
MS.gene64998	MS.gene017431	PPI
MS.gene64998	MS.gene70258	PPI
MS.gene39538	MS.gene64998	PPI
MS.gene64998	MS.gene65125	PPI
MS.gene78922	MS.gene64998	PPI
MS.gene64998	MS.gene056335	PPI
MS.gene64998	MS.gene58112	PPI
MS.gene64998	MS.gene011710	PPI
MS.gene071851	MS.gene64998	PPI
MS.gene64998	MS.gene23886	PPI
MS.gene28434	MS.gene64998	PPI
MS.gene64998	MS.gene015760	PPI
MS.gene64998	MS.gene89203	PPI
MS.gene64998	MS.gene63118	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64998.t1	MTR_4g097175	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene64998.t1	MTR_1g023630	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene64998.t1	MTR_8g061940	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene64998.t1	MTR_8g099430	99.265	136	1	0	1	136	1	136	4.84e-96	272
MS.gene64998.t1	MTR_4g097170	99.265	136	1	0	1	136	42	177	7.71e-95	271
MS.gene64998.t1	MTR_7g059070	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_5g029820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_5g029770	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_4g065990	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_4g088150	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_2g035230	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_8g092720	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_8g063500	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_8g103245	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_8g092820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene64998.t1	MTR_7g013610	97.059	136	4	0	1	136	100	235	2.63e-92	266
MS.gene64998.t1	MTR_2g082310	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene64998.t1	MTR_2g082340	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene64998.t1	MTR_2g082370	86.301	146	10	1	1	136	1	146	3.79e-84	243
MS.gene64998.t1	MTR_2g082400	85.294	136	18	2	1	135	1	135	1.39e-76	223
MS.gene64998.t1	MTR_7g013600	83.036	112	19	0	25	136	2	113	1.72e-62	187
MS.gene64998.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	3.79e-56	172
MS.gene64998.t1	MTR_1g023630	77.477	111	23	1	1	111	1	109	1.51e-54	167
MS.gene64998.t1	MTR_7g023880	60.150	133	39	3	3	134	18	137	2.40e-44	142
MS.gene64998.t1	MTR_5g024630	63.793	116	39	2	1	116	1	113	1.54e-42	136
MS.gene64998.t1	MTR_5g024530	65.455	110	15	2	19	128	68	154	6.25e-40	131
MS.gene64998.t1	MTR_0197s0100	96.970	66	2	0	1	66	1	66	1.84e-39	129
MS.gene64998.t1	MTR_8g027840	56.122	98	42	1	37	133	28	125	4.65e-31	108
MS.gene64998.t1	MTR_8g105150	59.783	92	3	2	37	127	11	69	1.24e-26	95.1
MS.gene64998.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	5.77e-20	80.5
MS.gene64998.t1	MTR_5g089120	68.085	47	15	0	82	128	6	52	1.58e-16	70.1
MS.gene64998.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.14e-15	66.6
MS.gene64998.t1	MTR_4g128040	72.973	37	10	0	79	115	20	56	6.10e-14	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64998.t1	AT5G10980	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene64998.t1	AT4G40030	100.000	136	0	0	1	136	29	164	2.04e-96	275
MS.gene64998.t1	AT5G65360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene64998.t1	AT5G10390	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene64998.t1	AT5G10400	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene64998.t1	AT3G27360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene64998.t1	AT1G09200	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene64998.t1	AT1G75600	96.324	136	5	0	1	136	1	136	1.02e-92	264
MS.gene64998.t1	AT1G13370	94.853	136	7	0	1	136	1	136	2.16e-91	261
MS.gene64998.t1	AT5G65350	92.647	136	10	0	1	136	1	136	1.27e-88	254
MS.gene64998.t1	AT1G19890	90.511	137	12	1	1	136	1	137	1.89e-85	246
MS.gene64998.t1	AT5G12910	71.111	135	34	2	1	135	1	130	1.15e-65	196
MS.gene64998.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene64998.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene64998.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene64998.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112

Find 42 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGCTGTTCTTGCACTTC+AGG	0.281295	8.4:-39674512	MS.gene64998:CDS
TCACGAAGAGCAACGGTTCC+AGG	0.316584	8.4:+39674643	None:intergenic
GGAAGCTGCTGAGGCTTATC+TGG	0.326898	8.4:-39674491	MS.gene64998:CDS
TATTCGTGGGGAACGTGCTT+AGG	0.341106	8.4:-39674383	None:intergenic
AGCAGATTTCCTTGCAGCTT+TGG	0.347069	8.4:+39674705	None:intergenic
CTTCCTTGGTGCTTTGCCGC+CGG	0.391014	8.4:+39674738	None:intergenic
TTCTTCACTCCGCCGGTGGT+TGG	0.414328	8.4:+39674682	None:intergenic
TTATCTGGTGGGATTGTTTG+AGG	0.425602	8.4:-39674476	MS.gene64998:CDS
TTGGTGGCGAGTTGCTTCCT+TGG	0.434612	8.4:+39674724	None:intergenic
AGCTTGCTCGCCGTATTCGT+GGG	0.438872	8.4:-39674396	MS.gene64998:CDS
ATTCGTGGGGAACGTGCTTA+GGG	0.442077	8.4:-39674382	None:intergenic
CAGCTTGCTCGCCGTATTCG+TGG	0.442485	8.4:-39674397	MS.gene64998:CDS
CCTTGGTGCTTTGCCGCCGG+TGG	0.443251	8.4:+39674741	None:intergenic
TGCACTTCAGGAAGCTGCTG+AGG	0.475820	8.4:-39674500	MS.gene64998:CDS
AGATTTCCTTGCAGCTTTGG+TGG	0.476244	8.4:+39674708	None:intergenic
AAGCACGTTCCCCACGAATA+CGG	0.482240	8.4:+39674386	None:intergenic
TCTTGTTCGTGAAATTGCTC+AGG	0.482836	8.4:-39674563	MS.gene64998:CDS
CAGTACTCTTCTGATACTTA+CGG	0.507336	8.4:+39674617	None:intergenic
AGGAAATCTGCTCCAACCAC+CGG	0.534350	8.4:-39674694	MS.gene64998:CDS
AAGCCACATCGTTATCGTCC+TGG	0.536207	8.4:-39674661	MS.gene64998:CDS
AATTGCTCAGGATTTCAAAA+CGG	0.537563	8.4:-39674551	MS.gene64998:CDS
CGAGCAAGCTGAATATCCTT+CGG	0.538912	8.4:+39674409	None:intergenic
GCTGCTGAGGCTTATCTGGT+GGG	0.545568	8.4:-39674487	MS.gene64998:CDS
TGGCTTCTTCACTCCGCCGG+TGG	0.548143	8.4:+39674678	None:intergenic
ATGTGGCTTCTTCACTCCGC+CGG	0.555659	8.4:+39674675	None:intergenic
AGCTGCTGAGGCTTATCTGG+TGG	0.558286	8.4:-39674488	MS.gene64998:CDS
GACGCTGGAATGGAAGCTTA+CGG	0.558442	8.4:+39674584	None:intergenic
TCACGAACAAGACGCTGGAA+TGG	0.567002	8.4:+39674574	None:intergenic
CAAACCGCTCGCAAATCCAC+CGG	0.582831	8.4:-39674757	MS.gene64998:CDS
AGGATTTCAAAACGGATCTG+AGG	0.592218	8.4:-39674543	MS.gene64998:CDS
ACGGATCTCACGAAGAGCAA+CGG	0.595480	8.4:+39674636	None:intergenic
AACTCGCCACCAAAGCTGCA+AGG	0.602982	8.4:-39674714	MS.gene64998:CDS
CAATTTCACGAACAAGACGC+TGG	0.604340	8.4:+39674569	None:intergenic
GTGTGCTATTCATGCTAAGA+GGG	0.612146	8.4:-39674443	MS.gene64998:CDS
GCTTGCTCGCCGTATTCGTG+GGG	0.613696	8.4:-39674395	MS.gene64998:CDS
TGTGTGCTATTCATGCTAAG+AGG	0.615466	8.4:-39674444	MS.gene64998:CDS
ACCGCTCGCAAATCCACCGG+CGG	0.623519	8.4:-39674754	MS.gene64998:CDS
GAGGGTGACGATTATGCCGA+AGG	0.625074	8.4:-39674425	MS.gene64998:CDS
CCACCGGCGGCAAAGCACCA+AGG	0.632902	8.4:-39674741	MS.gene64998:CDS
GCCGCCGGTGGATTTGCGAG+CGG	0.651481	8.4:+39674753	None:intergenic
AAATCTGCTCCAACCACCGG+CGG	0.687441	8.4:-39674691	MS.gene64998:CDS
GTTCCAGGACGATAACGATG+TGG	0.733691	8.4:+39674658	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTGCTCAGGATTTCAAAA+CGG	-	chr8.4:39674605-39674624	MS.gene64998:CDS	30.0%
	CAGTACTCTTCTGATACTTA+CGG	+	chr8.4:39674542-39674561	None:intergenic	35.0%
	AGGATTTCAAAACGGATCTG+AGG	-	chr8.4:39674613-39674632	MS.gene64998:CDS	40.0%
	TCTTGTTCGTGAAATTGCTC+AGG	-	chr8.4:39674593-39674612	MS.gene64998:CDS	40.0%
	TTATCTGGTGGGATTGTTTG+AGG	-	chr8.4:39674680-39674699	MS.gene64998:CDS	40.0%
!	GTGTGCTATTCATGCTAAGA+GGG	-	chr8.4:39674713-39674732	MS.gene64998:CDS	40.0%
!	TGTGTGCTATTCATGCTAAG+AGG	-	chr8.4:39674712-39674731	MS.gene64998:CDS	40.0%
	AGCAGATTTCCTTGCAGCTT+TGG	+	chr8.4:39674454-39674473	None:intergenic	45.0%
	CAATTTCACGAACAAGACGC+TGG	+	chr8.4:39674590-39674609	None:intergenic	45.0%
	CGAGCAAGCTGAATATCCTT+CGG	+	chr8.4:39674750-39674769	None:intergenic	45.0%
	TCATGCTGTTCTTGCACTTC+AGG	-	chr8.4:39674644-39674663	MS.gene64998:CDS	45.0%
!	AGATTTCCTTGCAGCTTTGG+TGG	+	chr8.4:39674451-39674470	None:intergenic	45.0%
	AAGCACGTTCCCCACGAATA+CGG	+	chr8.4:39674773-39674792	None:intergenic	50.0%
	AAGCCACATCGTTATCGTCC+TGG	-	chr8.4:39674495-39674514	MS.gene64998:CDS	50.0%
	ACGGATCTCACGAAGAGCAA+CGG	+	chr8.4:39674523-39674542	None:intergenic	50.0%
	AGGAAATCTGCTCCAACCAC+CGG	-	chr8.4:39674462-39674481	MS.gene64998:CDS	50.0%
	GACGCTGGAATGGAAGCTTA+CGG	+	chr8.4:39674575-39674594	None:intergenic	50.0%
	GTTCCAGGACGATAACGATG+TGG	+	chr8.4:39674501-39674520	None:intergenic	50.0%
	TCACGAACAAGACGCTGGAA+TGG	+	chr8.4:39674585-39674604	None:intergenic	50.0%
	AAATCTGCTCCAACCACCGG+CGG	-	chr8.4:39674465-39674484	MS.gene64998:CDS	55.0%
	AACTCGCCACCAAAGCTGCA+AGG	-	chr8.4:39674442-39674461	MS.gene64998:CDS	55.0%
	AGCTGCTGAGGCTTATCTGG+TGG	-	chr8.4:39674668-39674687	MS.gene64998:CDS	55.0%
	ATGTGGCTTCTTCACTCCGC+CGG	+	chr8.4:39674484-39674503	None:intergenic	55.0%
	CAAACCGCTCGCAAATCCAC+CGG	-	chr8.4:39674399-39674418	MS.gene64998:CDS	55.0%
	GAGGGTGACGATTATGCCGA+AGG	-	chr8.4:39674731-39674750	MS.gene64998:CDS	55.0%
	GCTGCTGAGGCTTATCTGGT+GGG	-	chr8.4:39674669-39674688	MS.gene64998:CDS	55.0%
	GGAAGCTGCTGAGGCTTATC+TGG	-	chr8.4:39674665-39674684	MS.gene64998:CDS	55.0%
	TCACGAAGAGCAACGGTTCC+AGG	+	chr8.4:39674516-39674535	None:intergenic	55.0%
	TGCACTTCAGGAAGCTGCTG+AGG	-	chr8.4:39674656-39674675	MS.gene64998:CDS	55.0%
!	AGCTTGCTCGCCGTATTCGT+GGG	-	chr8.4:39674760-39674779	MS.gene64998:CDS	55.0%
!!	TTGGTGGCGAGTTGCTTCCT+TGG	+	chr8.4:39674435-39674454	None:intergenic	55.0%
	GCTTGCTCGCCGTATTCGTG+GGG	-	chr8.4:39674761-39674780	MS.gene64998:CDS	60.0%
	TTCTTCACTCCGCCGGTGGT+TGG	+	chr8.4:39674477-39674496	None:intergenic	60.0%
!	CAGCTTGCTCGCCGTATTCG+TGG	-	chr8.4:39674759-39674778	MS.gene64998:CDS	60.0%
!!	CTTCCTTGGTGCTTTGCCGC+CGG	+	chr8.4:39674421-39674440	None:intergenic	60.0%
	ACCGCTCGCAAATCCACCGG+CGG	-	chr8.4:39674402-39674421	MS.gene64998:CDS	65.0%
	TGGCTTCTTCACTCCGCCGG+TGG	+	chr8.4:39674481-39674500	None:intergenic	65.0%
!	CCACCGGCGGCAAAGCACCA+AGG	-	chr8.4:39674415-39674434	MS.gene64998:CDS	70.0%
!	GCCGCCGGTGGATTTGCGAG+CGG	+	chr8.4:39674406-39674425	None:intergenic	70.0%
!!	CCTTGGTGCTTTGCCGCCGG+TGG	+	chr8.4:39674418-39674437	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	39674384	39674794	39674384	ID=MS.gene64998
chr8.4	mRNA	39674384	39674794	39674384	ID=MS.gene64998.t1;Parent=MS.gene64998
chr8.4	exon	39674384	39674794	39674384	ID=MS.gene64998.t1.exon1;Parent=MS.gene64998.t1
chr8.4	CDS	39674384	39674794	39674384	ID=cds.MS.gene64998.t1;Parent=MS.gene64998.t1

Gene Sequence

>MS.gene64998

ATGGCTCGTACGAAGCAAACCGCTCGCAAATCCACCGGCGGCAAAGCACCAAGGAAGCAACTCGCCACCAAAGCTGCAAGGAAATCTGCTCCAACCACCGGCGGAGTGAAGAAGCCACATCGTTATCGTCCTGGAACCGTTGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGTACTGAGCTTTTGATCCGTAAGCTTCCATTCCAGCGTCTTGTTCGTGAAATTGCTCAGGATTTCAAAACGGATCTGAGGTTTCAGAGTCATGCTGTTCTTGCACTTCAGGAAGCTGCTGAGGCTTATCTGGTGGGATTGTTTGAGGATACTAATTTGTGTGCTATTCATGCTAAGAGGGTGACGATTATGCCGAAGGATATTCAGCTTGCTCGCCGTATTCGTGGGGAACGTGCTTAG

Protein sequence

>MS.gene64998.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA