AlfalfaGEDB | MS.gene34797

M. sativa cultivar XinJiangDaYe / MS.gene34797

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34797.t1	NP_195713.1	100	136	0	0	1	136	1	136	8.90E-65	256.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34797.t1	P59169	100.0	136	0	0	1	136	1	136	2.1e-67	256.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34797.t1	A0A0V0RDG7	100.0	136	0	0	1	136	1	136	6.4e-65	256.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055694	MS.gene34797	0.822901	1.85E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34797	MS.gene017431	PPI
MS.gene34797	MS.gene78227	PPI
MS.gene34797	MS.gene70258	PPI
MS.gene038612	MS.gene34797	PPI
MS.gene027907	MS.gene34797	PPI
MS.gene34797	MS.gene011710	PPI
MS.gene64164	MS.gene34797	PPI
MS.gene24196	MS.gene34797	PPI
MS.gene58116	MS.gene34797	PPI
MS.gene64998	MS.gene34797	PPI
MS.gene030661	MS.gene34797	PPI
MS.gene61192	MS.gene34797	PPI
MS.gene34797	MS.gene63118	PPI
MS.gene28432	MS.gene34797	PPI
MS.gene34797	MS.gene78224	PPI
MS.gene89203	MS.gene34797	PPI
MS.gene056335	MS.gene34797	PPI
MS.gene068179	MS.gene34797	PPI
MS.gene97703	MS.gene34797	PPI
MS.gene91631	MS.gene34797	PPI
MS.gene009155	MS.gene34797	PPI
MS.gene78924	MS.gene34797	PPI
MS.gene25357	MS.gene34797	PPI
MS.gene34797	MS.gene28438	PPI
MS.gene34797	MS.gene64996	PPI
MS.gene39538	MS.gene34797	PPI
MS.gene78922	MS.gene34797	PPI
MS.gene071851	MS.gene34797	PPI
MS.gene28434	MS.gene34797	PPI
MS.gene34797	MS.gene04748	PPI
MS.gene017431	MS.gene34797	PPI
MS.gene059054	MS.gene34797	PPI
MS.gene70990	MS.gene34797	PPI
MS.gene05905	MS.gene34797	PPI
MS.gene60199	MS.gene34797	PPI
MS.gene021887	MS.gene34797	PPI
MS.gene34797	MS.gene70990	PPI
MS.gene26084	MS.gene34797	PPI
MS.gene44458	MS.gene34797	PPI
MS.gene34797	MS.gene027909	PPI
MS.gene34797	MS.gene70261	PPI
MS.gene47573	MS.gene34797	PPI
MS.gene030666	MS.gene34797	PPI
MS.gene34797	MS.gene030664	PPI
MS.gene97701	MS.gene34797	PPI
MS.gene057458	MS.gene34797	PPI
MS.gene049111	MS.gene34797	PPI
MS.gene027904	MS.gene34797	PPI
MS.gene00233	MS.gene34797	PPI
MS.gene34797	MS.gene05905	PPI
MS.gene068178	MS.gene34797	PPI
MS.gene44457	MS.gene34797	PPI
MS.gene34797	MS.gene58112	PPI
MS.gene66051	MS.gene34797	PPI
MS.gene011711	MS.gene34797	PPI
MS.gene65125	MS.gene34797	PPI
MS.gene29445	MS.gene34797	PPI
MS.gene23886	MS.gene34797	PPI
MS.gene26406	MS.gene34797	PPI
MS.gene60200	MS.gene34797	PPI
MS.gene015760	MS.gene34797	PPI
MS.gene58114	MS.gene34797	PPI
MS.gene34797	MS.gene60196	PPI
MS.gene34797	MS.gene61193	PPI
MS.gene70263	MS.gene34797	PPI
MS.gene58285	MS.gene34797	PPI
MS.gene28438	MS.gene34797	PPI
MS.gene34797	MS.gene26084	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34797.t1	MTR_4g097175	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene34797.t1	MTR_1g023630	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene34797.t1	MTR_8g061940	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene34797.t1	MTR_8g099430	99.265	136	1	0	1	136	1	136	4.84e-96	272
MS.gene34797.t1	MTR_4g097170	99.265	136	1	0	1	136	42	177	7.71e-95	271
MS.gene34797.t1	MTR_7g059070	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_5g029820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_5g029770	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_4g065990	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_4g088150	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_2g035230	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_8g092720	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_8g063500	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_8g103245	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_8g092820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene34797.t1	MTR_7g013610	97.059	136	4	0	1	136	100	235	2.63e-92	266
MS.gene34797.t1	MTR_2g082310	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene34797.t1	MTR_2g082340	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene34797.t1	MTR_2g082370	86.301	146	10	1	1	136	1	146	3.79e-84	243
MS.gene34797.t1	MTR_2g082400	85.294	136	18	2	1	135	1	135	1.39e-76	223
MS.gene34797.t1	MTR_7g013600	83.036	112	19	0	25	136	2	113	1.72e-62	187
MS.gene34797.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	3.79e-56	172
MS.gene34797.t1	MTR_1g023630	77.477	111	23	1	1	111	1	109	1.51e-54	167
MS.gene34797.t1	MTR_7g023880	60.150	133	39	3	3	134	18	137	2.40e-44	142
MS.gene34797.t1	MTR_5g024630	63.793	116	39	2	1	116	1	113	1.54e-42	136
MS.gene34797.t1	MTR_5g024530	65.455	110	15	2	19	128	68	154	6.25e-40	131
MS.gene34797.t1	MTR_0197s0100	96.970	66	2	0	1	66	1	66	1.84e-39	129
MS.gene34797.t1	MTR_8g027840	56.122	98	42	1	37	133	28	125	4.65e-31	108
MS.gene34797.t1	MTR_8g105150	59.783	92	3	2	37	127	11	69	1.24e-26	95.1
MS.gene34797.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	5.77e-20	80.5
MS.gene34797.t1	MTR_5g089120	68.085	47	15	0	82	128	6	52	1.58e-16	70.1
MS.gene34797.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.14e-15	66.6
MS.gene34797.t1	MTR_4g128040	72.973	37	10	0	79	115	20	56	6.10e-14	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34797.t1	AT5G10980	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene34797.t1	AT4G40030	100.000	136	0	0	1	136	29	164	2.04e-96	275
MS.gene34797.t1	AT5G65360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene34797.t1	AT5G10390	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene34797.t1	AT5G10400	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene34797.t1	AT3G27360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene34797.t1	AT1G09200	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene34797.t1	AT1G75600	96.324	136	5	0	1	136	1	136	1.02e-92	264
MS.gene34797.t1	AT1G13370	94.853	136	7	0	1	136	1	136	2.16e-91	261
MS.gene34797.t1	AT5G65350	92.647	136	10	0	1	136	1	136	1.27e-88	254
MS.gene34797.t1	AT1G19890	90.511	137	12	1	1	136	1	137	1.89e-85	246
MS.gene34797.t1	AT5G12910	71.111	135	34	2	1	135	1	130	1.15e-65	196
MS.gene34797.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene34797.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene34797.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene34797.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGCTGTTCTTGCACTTC+AGG	0.281295	8.3:-40158071	MS.gene34797:CDS
TATTCGTGGTGAACGTGCTT+AGG	0.304195	8.3:-40157942	None:intergenic
TCACGAAGAGCAACGGTTCC+AGG	0.316584	8.3:+40158202	None:intergenic
AGCAGATTTCCTTGCAGCTT+TGG	0.347069	8.3:+40158264	None:intergenic
TTCTTCACTCCGCCGGTTGT+TGG	0.348152	8.3:+40158241	None:intergenic
GGAAGCGGCTGAGGCTTATC+TGG	0.391484	8.3:-40158050	MS.gene34797:CDS
ATTCGTGGTGAACGTGCTTA+GGG	0.417330	8.3:-40157941	None:intergenic
TTATCTGGTGGGATTGTTTG+AGG	0.425602	8.3:-40158035	MS.gene34797:CDS
CAGCTTGCTCGTCGTATTCG+TGG	0.425806	8.3:-40157956	MS.gene34797:CDS
TGCACTTCAGGAAGCGGCTG+AGG	0.430700	8.3:-40158059	MS.gene34797:CDS
TTGGTGGCGAGTTGCTTCCT+TGG	0.434612	8.3:+40158283	None:intergenic
AGGAAATCTGCTCCAACAAC+CGG	0.463576	8.3:-40158253	MS.gene34797:CDS
CCTTGGTGCCTTGCCGCCAG+TGG	0.465838	8.3:+40158300	None:intergenic
AGATTTCCTTGCAGCTTTGG+TGG	0.476244	8.3:+40158267	None:intergenic
TCTTGTTCGTGAAATTGCTC+AGG	0.482836	8.3:-40158122	MS.gene34797:CDS
CGGTACTCTTCTGATACTTA+CGG	0.500491	8.3:+40158176	None:intergenic
AAGCCTCATCGTTATCGTCC+TGG	0.515117	8.3:-40158220	MS.gene34797:CDS
TCGCAAATCCACTGGCGGCA+AGG	0.534048	8.3:-40158308	MS.gene34797:CDS
AATTGCTCAGGATTTCAAAA+CGG	0.537563	8.3:-40158110	MS.gene34797:CDS
CAAACCGCTCGCAAATCCAC+TGG	0.548661	8.3:-40158316	MS.gene34797:CDS
ATGAGGCTTCTTCACTCCGC+CGG	0.553886	8.3:+40158234	None:intergenic
GACGCTGGAATGGAAGCTTA+CGG	0.558442	8.3:+40158143	None:intergenic
TGTTCTTGCACTTCAGGAAG+CGG	0.559874	8.3:-40158065	MS.gene34797:CDS
CGAGCAAGCTGAATATCCTT+CGG	0.565564	8.3:+40157968	None:intergenic
TCACGAACAAGACGCTGGAA+TGG	0.567002	8.3:+40158133	None:intergenic
GCGGCTGAGGCTTATCTGGT+GGG	0.567245	8.3:-40158046	MS.gene34797:CDS
AGCGGCTGAGGCTTATCTGG+TGG	0.574844	8.3:-40158047	MS.gene34797:CDS
AGGATTTCAAAACGGATCTG+AGG	0.592218	8.3:-40158102	MS.gene34797:CDS
ACGGATCTCACGAAGAGCAA+CGG	0.595480	8.3:+40158195	None:intergenic
ACCGCTCGCAAATCCACTGG+CGG	0.602838	8.3:-40158313	MS.gene34797:CDS
AACTCGCCACCAAAGCTGCA+AGG	0.602982	8.3:-40158273	MS.gene34797:CDS
CAATTTCACGAACAAGACGC+TGG	0.604340	8.3:+40158128	None:intergenic
GTGTGCTATTCATGCTAAGA+GGG	0.612146	8.3:-40158002	MS.gene34797:CDS
TGTGTGCTATTCATGCTAAG+AGG	0.615466	8.3:-40158003	MS.gene34797:CDS
GAGGGTGACGATTATGCCGA+AGG	0.625074	8.3:-40157984	MS.gene34797:CDS
GCCGCCAGTGGATTTGCGAG+CGG	0.626858	8.3:+40158312	None:intergenic
AAGCTTACGGATCAAAAGCT+CGG	0.636318	8.3:+40158156	None:intergenic
CCACTGGCGGCAAGGCACCA+AGG	0.643617	8.3:-40158300	MS.gene34797:CDS
AAATCTGCTCCAACAACCGG+CGG	0.700123	8.3:-40158250	MS.gene34797:CDS
GTTCCAGGACGATAACGATG+AGG	0.738178	8.3:+40158217	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTGCTCAGGATTTCAAAA+CGG	-	chr8.3:40158164-40158183	MS.gene34797:CDS	30.0%
	AAGCTTACGGATCAAAAGCT+CGG	+	chr8.3:40158121-40158140	None:intergenic	40.0%
	AGGATTTCAAAACGGATCTG+AGG	-	chr8.3:40158172-40158191	MS.gene34797:CDS	40.0%
	CGGTACTCTTCTGATACTTA+CGG	+	chr8.3:40158101-40158120	None:intergenic	40.0%
	TCTTGTTCGTGAAATTGCTC+AGG	-	chr8.3:40158152-40158171	MS.gene34797:CDS	40.0%
	TTATCTGGTGGGATTGTTTG+AGG	-	chr8.3:40158239-40158258	MS.gene34797:CDS	40.0%
!	GTGTGCTATTCATGCTAAGA+GGG	-	chr8.3:40158272-40158291	MS.gene34797:CDS	40.0%
!	TGTGTGCTATTCATGCTAAG+AGG	-	chr8.3:40158271-40158290	MS.gene34797:CDS	40.0%
	AGCAGATTTCCTTGCAGCTT+TGG	+	chr8.3:40158013-40158032	None:intergenic	45.0%
	AGGAAATCTGCTCCAACAAC+CGG	-	chr8.3:40158021-40158040	MS.gene34797:CDS	45.0%
	CAATTTCACGAACAAGACGC+TGG	+	chr8.3:40158149-40158168	None:intergenic	45.0%
	CGAGCAAGCTGAATATCCTT+CGG	+	chr8.3:40158309-40158328	None:intergenic	45.0%
	TCATGCTGTTCTTGCACTTC+AGG	-	chr8.3:40158203-40158222	MS.gene34797:CDS	45.0%
	TGTTCTTGCACTTCAGGAAG+CGG	-	chr8.3:40158209-40158228	MS.gene34797:CDS	45.0%
!	AGATTTCCTTGCAGCTTTGG+TGG	+	chr8.3:40158010-40158029	None:intergenic	45.0%
	AAATCTGCTCCAACAACCGG+CGG	-	chr8.3:40158024-40158043	MS.gene34797:CDS	50.0%
	AAGCCTCATCGTTATCGTCC+TGG	-	chr8.3:40158054-40158073	MS.gene34797:CDS	50.0%
	ACGGATCTCACGAAGAGCAA+CGG	+	chr8.3:40158082-40158101	None:intergenic	50.0%
	GACGCTGGAATGGAAGCTTA+CGG	+	chr8.3:40158134-40158153	None:intergenic	50.0%
	GTTCCAGGACGATAACGATG+AGG	+	chr8.3:40158060-40158079	None:intergenic	50.0%
	TCACGAACAAGACGCTGGAA+TGG	+	chr8.3:40158144-40158163	None:intergenic	50.0%
	AACTCGCCACCAAAGCTGCA+AGG	-	chr8.3:40158001-40158020	MS.gene34797:CDS	55.0%
	ATGAGGCTTCTTCACTCCGC+CGG	+	chr8.3:40158043-40158062	None:intergenic	55.0%
	CAAACCGCTCGCAAATCCAC+TGG	-	chr8.3:40157958-40157977	MS.gene34797:CDS	55.0%
	GAGGGTGACGATTATGCCGA+AGG	-	chr8.3:40158290-40158309	MS.gene34797:CDS	55.0%
	TCACGAAGAGCAACGGTTCC+AGG	+	chr8.3:40158075-40158094	None:intergenic	55.0%
!	CAGCTTGCTCGTCGTATTCG+TGG	-	chr8.3:40158318-40158337	MS.gene34797:CDS	55.0%
!	TTCTTCACTCCGCCGGTTGT+TGG	+	chr8.3:40158036-40158055	None:intergenic	55.0%
!!	TTGGTGGCGAGTTGCTTCCT+TGG	+	chr8.3:40157994-40158013	None:intergenic	55.0%
	ACCGCTCGCAAATCCACTGG+CGG	-	chr8.3:40157961-40157980	MS.gene34797:CDS	60.0%
	AGCGGCTGAGGCTTATCTGG+TGG	-	chr8.3:40158227-40158246	MS.gene34797:CDS	60.0%
	GCGGCTGAGGCTTATCTGGT+GGG	-	chr8.3:40158228-40158247	MS.gene34797:CDS	60.0%
	GGAAGCGGCTGAGGCTTATC+TGG	-	chr8.3:40158224-40158243	MS.gene34797:CDS	60.0%
	TCGCAAATCCACTGGCGGCA+AGG	-	chr8.3:40157966-40157985	MS.gene34797:CDS	60.0%
	TGCACTTCAGGAAGCGGCTG+AGG	-	chr8.3:40158215-40158234	MS.gene34797:CDS	60.0%
!	GCCGCCAGTGGATTTGCGAG+CGG	+	chr8.3:40157965-40157984	None:intergenic	65.0%
!	CCACTGGCGGCAAGGCACCA+AGG	-	chr8.3:40157974-40157993	MS.gene34797:CDS	70.0%
!!	CCTTGGTGCCTTGCCGCCAG+TGG	+	chr8.3:40157977-40157996	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	40157943	40158353	40157943	ID=MS.gene34797
chr8.3	mRNA	40157943	40158353	40157943	ID=MS.gene34797.t1;Parent=MS.gene34797
chr8.3	exon	40157943	40158353	40157943	ID=MS.gene34797.t1.exon1;Parent=MS.gene34797.t1
chr8.3	CDS	40157943	40158353	40157943	ID=cds.MS.gene34797.t1;Parent=MS.gene34797.t1

Gene Sequence

>MS.gene34797

ATGGCTCGTACGAAGCAAACCGCTCGCAAATCCACTGGCGGCAAGGCACCAAGGAAGCAACTCGCCACCAAAGCTGCAAGGAAATCTGCTCCAACAACCGGCGGAGTGAAGAAGCCTCATCGTTATCGTCCTGGAACCGTTGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGTACCGAGCTTTTGATCCGTAAGCTTCCATTCCAGCGTCTTGTTCGTGAAATTGCTCAGGATTTCAAAACGGATCTGAGGTTTCAGAGTCATGCTGTTCTTGCACTTCAGGAAGCGGCTGAGGCTTATCTGGTGGGATTGTTTGAGGATACTAATTTGTGTGCTATTCATGCTAAGAGGGTGACGATTATGCCGAAGGATATTCAGCTTGCTCGTCGTATTCGTGGTGAACGTGCTTAG

Protein sequence

>MS.gene34797.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA