AlfalfaGEDB | MS.gene63118

M. sativa cultivar XinJiangDaYe / MS.gene63118

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63118.t1	NP_195713.1	100	136	0	0	1	136	1	136	8.90E-65	256.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63118.t1	P59169	100.0	136	0	0	1	136	1	136	2.1e-67	256.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63118.t1	A0A0V0RDG7	100.0	136	0	0	1	136	1	136	6.4e-65	256.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene61192	MS.gene63118	PPI
MS.gene29445	MS.gene63118	PPI
MS.gene63118	MS.gene78224	PPI
MS.gene26406	MS.gene63118	PPI
MS.gene58112	MS.gene63118	PPI
MS.gene89203	MS.gene63118	PPI
MS.gene056335	MS.gene63118	PPI
MS.gene068179	MS.gene63118	PPI
MS.gene60196	MS.gene63118	PPI
MS.gene58114	MS.gene63118	PPI
MS.gene78227	MS.gene63118	PPI
MS.gene34797	MS.gene63118	PPI
MS.gene78924	MS.gene63118	PPI
MS.gene25357	MS.gene63118	PPI
MS.gene63118	MS.gene70261	PPI
MS.gene038612	MS.gene63118	PPI
MS.gene027907	MS.gene63118	PPI
MS.gene64164	MS.gene63118	PPI
MS.gene28434	MS.gene63118	PPI
MS.gene24196	MS.gene63118	PPI
MS.gene58116	MS.gene63118	PPI
MS.gene63118	MS.gene04748	PPI
MS.gene030661	MS.gene63118	PPI
MS.gene28432	MS.gene63118	PPI
MS.gene017431	MS.gene63118	PPI
MS.gene64996	MS.gene63118	PPI
MS.gene97703	MS.gene63118	PPI
MS.gene059054	MS.gene63118	PPI
MS.gene70258	MS.gene63118	PPI
MS.gene70990	MS.gene63118	PPI
MS.gene91631	MS.gene63118	PPI
MS.gene60199	MS.gene63118	PPI
MS.gene021887	MS.gene63118	PPI
MS.gene009155	MS.gene63118	PPI
MS.gene26084	MS.gene63118	PPI
MS.gene44458	MS.gene63118	PPI
MS.gene63118	MS.gene027909	PPI
MS.gene39538	MS.gene63118	PPI
MS.gene78922	MS.gene63118	PPI
MS.gene61193	MS.gene63118	PPI
MS.gene071851	MS.gene63118	PPI
MS.gene47573	MS.gene63118	PPI
MS.gene78224	MS.gene63118	PPI
MS.gene70261	MS.gene63118	PPI
MS.gene030666	MS.gene63118	PPI
MS.gene97701	MS.gene63118	PPI
MS.gene057458	MS.gene63118	PPI
MS.gene030664	MS.gene63118	PPI
MS.gene027904	MS.gene63118	PPI
MS.gene00233	MS.gene63118	PPI
MS.gene05905	MS.gene63118	PPI
MS.gene068178	MS.gene63118	PPI
MS.gene44457	MS.gene63118	PPI
MS.gene04748	MS.gene63118	PPI
MS.gene63118	MS.gene58112	PPI
MS.gene63118	MS.gene011710	PPI
MS.gene66051	MS.gene63118	PPI
MS.gene65125	MS.gene63118	PPI
MS.gene027909	MS.gene63118	PPI
MS.gene23886	MS.gene63118	PPI
MS.gene60200	MS.gene63118	PPI
MS.gene015760	MS.gene63118	PPI
MS.gene049111	MS.gene63118	PPI
MS.gene011710	MS.gene63118	PPI
MS.gene70263	MS.gene63118	PPI
MS.gene58285	MS.gene63118	PPI
MS.gene28438	MS.gene63118	PPI
MS.gene011711	MS.gene63118	PPI
MS.gene64998	MS.gene63118	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63118.t1	MTR_4g097175	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene63118.t1	MTR_1g023630	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene63118.t1	MTR_8g061940	100.000	136	0	0	1	136	1	136	1.33e-96	274
MS.gene63118.t1	MTR_8g099430	99.265	136	1	0	1	136	1	136	4.84e-96	272
MS.gene63118.t1	MTR_4g097170	99.265	136	1	0	1	136	42	177	7.71e-95	271
MS.gene63118.t1	MTR_7g059070	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_5g029820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_5g029770	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_4g065990	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_4g088150	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_2g035230	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_8g092720	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_8g063500	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_8g103245	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_8g092820	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene63118.t1	MTR_7g013610	97.059	136	4	0	1	136	100	235	2.63e-92	266
MS.gene63118.t1	MTR_2g082310	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene63118.t1	MTR_2g082340	92.647	136	10	0	1	136	1	136	4.16e-90	257
MS.gene63118.t1	MTR_2g082370	86.301	146	10	1	1	136	1	146	3.79e-84	243
MS.gene63118.t1	MTR_2g082400	85.294	136	18	2	1	135	1	135	1.39e-76	223
MS.gene63118.t1	MTR_7g013600	83.036	112	19	0	25	136	2	113	1.72e-62	187
MS.gene63118.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	3.79e-56	172
MS.gene63118.t1	MTR_1g023630	77.477	111	23	1	1	111	1	109	1.51e-54	167
MS.gene63118.t1	MTR_7g023880	60.150	133	39	3	3	134	18	137	2.40e-44	142
MS.gene63118.t1	MTR_5g024630	63.793	116	39	2	1	116	1	113	1.54e-42	136
MS.gene63118.t1	MTR_5g024530	65.455	110	15	2	19	128	68	154	6.25e-40	131
MS.gene63118.t1	MTR_0197s0100	96.970	66	2	0	1	66	1	66	1.84e-39	129
MS.gene63118.t1	MTR_8g027840	56.122	98	42	1	37	133	28	125	4.65e-31	108
MS.gene63118.t1	MTR_8g105150	59.783	92	3	2	37	127	11	69	1.24e-26	95.1
MS.gene63118.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	5.77e-20	80.5
MS.gene63118.t1	MTR_5g089120	68.085	47	15	0	82	128	6	52	1.58e-16	70.1
MS.gene63118.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.14e-15	66.6
MS.gene63118.t1	MTR_4g128040	72.973	37	10	0	79	115	20	56	6.10e-14	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63118.t1	AT5G10980	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40040	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40030	100.000	136	0	0	1	136	1	136	1.39e-96	274
MS.gene63118.t1	AT4G40030	100.000	136	0	0	1	136	29	164	2.04e-96	275
MS.gene63118.t1	AT5G65360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene63118.t1	AT5G10390	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene63118.t1	AT5G10400	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene63118.t1	AT3G27360	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene63118.t1	AT1G09200	97.059	136	4	0	1	136	1	136	3.84e-93	265
MS.gene63118.t1	AT1G75600	96.324	136	5	0	1	136	1	136	1.02e-92	264
MS.gene63118.t1	AT1G13370	94.853	136	7	0	1	136	1	136	2.16e-91	261
MS.gene63118.t1	AT5G65350	92.647	136	10	0	1	136	1	136	1.27e-88	254
MS.gene63118.t1	AT1G19890	90.511	137	12	1	1	136	1	137	1.89e-85	246
MS.gene63118.t1	AT5G12910	71.111	135	34	2	1	135	1	130	1.15e-65	196
MS.gene63118.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene63118.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene63118.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112
MS.gene63118.t1	AT1G01370	54.867	113	48	2	24	134	63	174	3.24e-32	112

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGCTGTTCTTGCACTTC+AGG	0.281295	8.1:-43217245	MS.gene63118:CDS
TATTCGTGGTGAACGTGCTT+AGG	0.304195	8.1:-43217116	None:intergenic
TCACGAAGAGCAACGGTTCC+AGG	0.316584	8.1:+43217376	None:intergenic
TTCTTCACTCCGCCGGTGGT+TGG	0.414328	8.1:+43217415	None:intergenic
CTTCCTTGGTGCCTTGCCGC+CGG	0.416556	8.1:+43217471	None:intergenic
ATTCGTGGTGAACGTGCTTA+GGG	0.417330	8.1:-43217115	None:intergenic
TTATCTCGTGGGATTGTTTG+AGG	0.422089	8.1:-43217209	MS.gene63118:CDS
CAGCTTGCTCGTCGTATTCG+TGG	0.425806	8.1:-43217130	MS.gene63118:CDS
AGCGGATTTCCTAGCAGCTT+TGG	0.427537	8.1:+43217438	None:intergenic
TTGGTGGCGAGTTGCTTCCT+TGG	0.434612	8.1:+43217457	None:intergenic
CCTTGGTGCCTTGCCGCCGG+TGG	0.468076	8.1:+43217474	None:intergenic
TGCACTTCAGGAAGCTGCTG+AGG	0.475820	8.1:-43217233	MS.gene63118:CDS
TCTTGTTCGTGAAATTGCTC+AGG	0.482836	8.1:-43217296	MS.gene63118:CDS
GGATTTCCTAGCAGCTTTGG+TGG	0.499313	8.1:+43217441	None:intergenic
CGGTACTCTTCTGATACTTA+CGG	0.500491	8.1:+43217350	None:intergenic
CACTCCGCCGGTGGTTGGAG+CGG	0.512593	8.1:+43217420	None:intergenic
AAGCCTCATCGTTATCGTCC+TGG	0.515117	8.1:-43217394	MS.gene63118:CDS
AGGAAATCCGCTCCAACCAC+CGG	0.521609	8.1:-43217427	MS.gene63118:CDS
GTGTGCTATTCATGCTAAGA+GGG	0.528359	8.1:-43217176	MS.gene63118:CDS
TCGCAAATCCACCGGCGGCA+AGG	0.530602	8.1:-43217482	MS.gene63118:CDS
AATTGCTCAGGATTTCAAAA+CGG	0.537563	8.1:-43217284	MS.gene63118:CDS
TGTGTGCTATTCATGCTAAG+AGG	0.552074	8.1:-43217177	MS.gene63118:CDS
AGGCTTCTTCACTCCGCCGG+TGG	0.558370	8.1:+43217411	None:intergenic
GACGCTGGAATGGAAGCTTA+CGG	0.558442	8.1:+43217317	None:intergenic
AGCTGCTGAGGCTTATCTCG+TGG	0.563702	8.1:-43217221	MS.gene63118:CDS
CGAGCAAGCTGAATATCCTT+CGG	0.565564	8.1:+43217142	None:intergenic
TCACGAACAAGACGCTGGAA+TGG	0.567002	8.1:+43217307	None:intergenic
ATGAGGCTTCTTCACTCCGC+CGG	0.573608	8.1:+43217408	None:intergenic
GCTGCTGAGGCTTATCTCGT+GGG	0.575716	8.1:-43217220	MS.gene63118:CDS
CCACCGGCGGCAAGGCACCA+AGG	0.578820	8.1:-43217474	MS.gene63118:CDS
CAAACCGCTCGCAAATCCAC+CGG	0.582831	8.1:-43217490	MS.gene63118:CDS
AACTCGCCACCAAAGCTGCT+AGG	0.589915	8.1:-43217447	MS.gene63118:CDS
ACGGATCTCACGAAGAGCAA+CGG	0.595480	8.1:+43217369	None:intergenic
CAATTTCACGAACAAGACGC+TGG	0.604340	8.1:+43217302	None:intergenic
GAGGGTTACGATTATGCCGA+AGG	0.616739	8.1:-43217158	MS.gene63118:CDS
ACCGCTCGCAAATCCACCGG+CGG	0.623519	8.1:-43217487	MS.gene63118:CDS
AAGCTTACGGATCAAAAGCT+CGG	0.636318	8.1:+43217330	None:intergenic
GCCGCCGGTGGATTTGCGAG+CGG	0.651481	8.1:+43217486	None:intergenic
AAATCCGCTCCAACCACCGG+CGG	0.686585	8.1:-43217424	MS.gene63118:CDS
GTTCCAGGACGATAACGATG+AGG	0.738178	8.1:+43217391	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTGCTCAGGATTTCAAAA+CGG	-	chr8.1:43217338-43217357	MS.gene63118:CDS	30.0%
	AAGCTTACGGATCAAAAGCT+CGG	+	chr8.1:43217295-43217314	None:intergenic	40.0%
	CGGTACTCTTCTGATACTTA+CGG	+	chr8.1:43217275-43217294	None:intergenic	40.0%
	TCTTGTTCGTGAAATTGCTC+AGG	-	chr8.1:43217326-43217345	MS.gene63118:CDS	40.0%
	TTATCTCGTGGGATTGTTTG+AGG	-	chr8.1:43217413-43217432	MS.gene63118:CDS	40.0%
!	GTGTGCTATTCATGCTAAGA+GGG	-	chr8.1:43217446-43217465	MS.gene63118:CDS	40.0%
!	TGTGTGCTATTCATGCTAAG+AGG	-	chr8.1:43217445-43217464	MS.gene63118:CDS	40.0%
	CAATTTCACGAACAAGACGC+TGG	+	chr8.1:43217323-43217342	None:intergenic	45.0%
	CGAGCAAGCTGAATATCCTT+CGG	+	chr8.1:43217483-43217502	None:intergenic	45.0%
	TCATGCTGTTCTTGCACTTC+AGG	-	chr8.1:43217377-43217396	MS.gene63118:CDS	45.0%
	AAGCCTCATCGTTATCGTCC+TGG	-	chr8.1:43217228-43217247	MS.gene63118:CDS	50.0%
	ACGGATCTCACGAAGAGCAA+CGG	+	chr8.1:43217256-43217275	None:intergenic	50.0%
	GACGCTGGAATGGAAGCTTA+CGG	+	chr8.1:43217308-43217327	None:intergenic	50.0%
	GAGGGTTACGATTATGCCGA+AGG	-	chr8.1:43217464-43217483	MS.gene63118:CDS	50.0%
	GTTCCAGGACGATAACGATG+AGG	+	chr8.1:43217234-43217253	None:intergenic	50.0%
	TCACGAACAAGACGCTGGAA+TGG	+	chr8.1:43217318-43217337	None:intergenic	50.0%
!	AGCGGATTTCCTAGCAGCTT+TGG	+	chr8.1:43217187-43217206	None:intergenic	50.0%
!	GGATTTCCTAGCAGCTTTGG+TGG	+	chr8.1:43217184-43217203	None:intergenic	50.0%
	AACTCGCCACCAAAGCTGCT+AGG	-	chr8.1:43217175-43217194	MS.gene63118:CDS	55.0%
	AGCTGCTGAGGCTTATCTCG+TGG	-	chr8.1:43217401-43217420	MS.gene63118:CDS	55.0%
	AGGAAATCCGCTCCAACCAC+CGG	-	chr8.1:43217195-43217214	MS.gene63118:CDS	55.0%
	ATGAGGCTTCTTCACTCCGC+CGG	+	chr8.1:43217217-43217236	None:intergenic	55.0%
	CAAACCGCTCGCAAATCCAC+CGG	-	chr8.1:43217132-43217151	MS.gene63118:CDS	55.0%
	GCTGCTGAGGCTTATCTCGT+GGG	-	chr8.1:43217402-43217421	MS.gene63118:CDS	55.0%
	TCACGAAGAGCAACGGTTCC+AGG	+	chr8.1:43217249-43217268	None:intergenic	55.0%
	TGCACTTCAGGAAGCTGCTG+AGG	-	chr8.1:43217389-43217408	MS.gene63118:CDS	55.0%
!	CAGCTTGCTCGTCGTATTCG+TGG	-	chr8.1:43217492-43217511	MS.gene63118:CDS	55.0%
!!	TTGGTGGCGAGTTGCTTCCT+TGG	+	chr8.1:43217168-43217187	None:intergenic	55.0%
	AAATCCGCTCCAACCACCGG+CGG	-	chr8.1:43217198-43217217	MS.gene63118:CDS	60.0%
	TTCTTCACTCCGCCGGTGGT+TGG	+	chr8.1:43217210-43217229	None:intergenic	60.0%
	ACCGCTCGCAAATCCACCGG+CGG	-	chr8.1:43217135-43217154	MS.gene63118:CDS	65.0%
	AGGCTTCTTCACTCCGCCGG+TGG	+	chr8.1:43217214-43217233	None:intergenic	65.0%
	TCGCAAATCCACCGGCGGCA+AGG	-	chr8.1:43217140-43217159	MS.gene63118:CDS	65.0%
!!	CTTCCTTGGTGCCTTGCCGC+CGG	+	chr8.1:43217154-43217173	None:intergenic	65.0%
	CACTCCGCCGGTGGTTGGAG+CGG	+	chr8.1:43217205-43217224	None:intergenic	70.0%
!	GCCGCCGGTGGATTTGCGAG+CGG	+	chr8.1:43217139-43217158	None:intergenic	70.0%
!	CCACCGGCGGCAAGGCACCA+AGG	-	chr8.1:43217148-43217167	MS.gene63118:CDS	75.0%
!!	CCTTGGTGCCTTGCCGCCGG+TGG	+	chr8.1:43217151-43217170	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	43217117	43217527	43217117	ID=MS.gene63118
chr8.1	mRNA	43217117	43217527	43217117	ID=MS.gene63118.t1;Parent=MS.gene63118
chr8.1	exon	43217117	43217527	43217117	ID=MS.gene63118.t1.exon1;Parent=MS.gene63118.t1
chr8.1	CDS	43217117	43217527	43217117	ID=cds.MS.gene63118.t1;Parent=MS.gene63118.t1

Gene Sequence

>MS.gene63118

ATGGCTCGTACGAAGCAAACCGCTCGCAAATCCACCGGCGGCAAGGCACCAAGGAAGCAACTCGCCACCAAAGCTGCTAGGAAATCCGCTCCAACCACCGGCGGAGTGAAGAAGCCTCATCGTTATCGTCCTGGAACCGTTGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGTACCGAGCTTTTGATCCGTAAGCTTCCATTCCAGCGTCTTGTTCGTGAAATTGCTCAGGATTTCAAAACGGATCTAAGATTTCAGAGTCATGCTGTTCTTGCACTTCAGGAAGCTGCTGAGGCTTATCTCGTGGGATTGTTTGAGGATACTAATTTGTGTGCTATTCATGCTAAGAGGGTTACGATTATGCCGAAGGATATTCAGCTTGCTCGTCGTATTCGTGGTGAACGTGCTTAG

Protein sequence

>MS.gene63118.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA