Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28802.t1 | XP_003625958.1 | 94.9 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 9.10E-103 | 382.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28802.t1 | G7KSS5 | 94.9 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 6.6e-103 | 382.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene28802 | -0.833992 | 3.88E-56 | -1.69E-46 |
| MS.gene04921 | MS.gene28802 | 0.828693 | 7.80E-55 | -1.69E-46 |
| MS.gene049633 | MS.gene28802 | -0.814652 | 1.38E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene28802 | -0.860033 | 2.74E-63 | -1.69E-46 |
| MS.gene050323 | MS.gene28802 | -0.82035 | 7.19E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene28802 | -0.836529 | 8.87E-57 | -1.69E-46 |
| MS.gene050504 | MS.gene28802 | -0.840561 | 8.07E-58 | -1.69E-46 |
| MS.gene050577 | MS.gene28802 | -0.84604 | 2.79E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene28802 | -0.83307 | 6.59E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene28802 | -0.803066 | 4.16E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene28802 | -0.826758 | 2.28E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene28802 | -0.82443 | 8.11E-54 | -1.69E-46 |
| MS.gene050904 | MS.gene28802 | -0.846514 | 2.07E-59 | -1.69E-46 |
| MS.gene051044 | MS.gene28802 | 0.807178 | 5.74E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene28802 | 0.820897 | 5.38E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene28802 | 0.829984 | 3.79E-55 | -1.69E-46 |
| MS.gene051317 | MS.gene28802 | -0.82716 | 1.82E-54 | -1.69E-46 |
| MS.gene051364 | MS.gene28802 | 0.81106 | 8.46E-51 | -1.69E-46 |
| MS.gene051366 | MS.gene28802 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene051422 | MS.gene28802 | 0.811233 | 7.76E-51 | -1.69E-46 |
| MS.gene051440 | MS.gene28802 | 0.817089 | 3.95E-52 | -1.69E-46 |
| MS.gene051651 | MS.gene28802 | 0.867383 | 1.43E-65 | -1.69E-46 |
| MS.gene051973 | MS.gene28802 | 0.816639 | 4.99E-52 | -1.69E-46 |
| MS.gene052015 | MS.gene28802 | 0.831994 | 1.22E-55 | -1.69E-46 |
| MS.gene052486 | MS.gene28802 | -0.805594 | 1.24E-49 | -1.69E-46 |
| MS.gene052494 | MS.gene28802 | 0.854038 | 1.61E-61 | -1.69E-46 |
| MS.gene052606 | MS.gene28802 | 0.837409 | 5.29E-57 | -1.69E-46 |
| MS.gene053097 | MS.gene28802 | 0.834986 | 2.18E-56 | -1.69E-46 |
| MS.gene053203 | MS.gene28802 | -0.8165 | 5.35E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene28802 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene28802 | -0.817913 | 2.58E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene28802 | 0.800189 | 1.62E-48 | -1.69E-46 |
| MS.gene053887 | MS.gene28802 | 0.800037 | 1.74E-48 | -1.69E-46 |
| MS.gene054706 | MS.gene28802 | 0.808207 | 3.47E-50 | -1.69E-46 |
| MS.gene054707 | MS.gene28802 | 0.809953 | 1.47E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene28802 | -0.85212 | 5.69E-61 | -1.69E-46 |
| MS.gene054954 | MS.gene28802 | -0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene28802 | 0.807645 | 4.57E-50 | -1.69E-46 |
| MS.gene055202 | MS.gene28802 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene055251 | MS.gene28802 | 0.804917 | 1.72E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene28802 | -0.804592 | 2.01E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene28802 | -0.829199 | 5.88E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene28802 | -0.815535 | 8.80E-52 | -1.69E-46 |
| MS.gene055731 | MS.gene28802 | 0.802086 | 6.63E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene28802 | -0.832117 | 1.13E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene28802 | -0.816182 | 6.31E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene28802 | -0.834911 | 2.28E-56 | -1.69E-46 |
| MS.gene05589 | MS.gene28802 | -0.880829 | 3.98E-70 | -1.69E-46 |
| MS.gene056198 | MS.gene28802 | 0.808008 | 3.82E-50 | -1.69E-46 |
| MS.gene056224 | MS.gene28802 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene056266 | MS.gene28802 | -0.810457 | 1.14E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene28802 | -0.860561 | 1.90E-63 | -1.69E-46 |
| MS.gene056692 | MS.gene28802 | -0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene28802 | 0.840739 | 7.25E-58 | -1.69E-46 |
| MS.gene056748 | MS.gene28802 | 0.815208 | 1.04E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene28802 | 0.818428 | 1.97E-52 | -1.69E-46 |
| MS.gene056988 | MS.gene28802 | 0.816877 | 4.41E-52 | -1.69E-46 |
| MS.gene057768 | MS.gene28802 | 0.870327 | 1.59E-66 | -1.69E-46 |
| MS.gene058068 | MS.gene28802 | 0.802219 | 6.22E-49 | -1.69E-46 |
| MS.gene058111 | MS.gene28802 | 0.839153 | 1.88E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene28802 | 0.840046 | 1.10E-57 | -1.69E-46 |
| MS.gene058173 | MS.gene28802 | 0.832961 | 7.01E-56 | -1.69E-46 |
| MS.gene058175 | MS.gene28802 | 0.843998 | 9.92E-59 | -1.69E-46 |
| MS.gene058334 | MS.gene28802 | 0.833548 | 5.01E-56 | -1.69E-46 |
| MS.gene05837 | MS.gene28802 | -0.830808 | 2.38E-55 | -1.69E-46 |
| MS.gene058382 | MS.gene28802 | 0.804715 | 1.89E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene28802 | -0.802835 | 4.64E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene28802 | -0.817984 | 2.48E-52 | -1.69E-46 |
| MS.gene058603 | MS.gene28802 | 0.808848 | 2.53E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene28802 | -0.809246 | 2.08E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene28802 | -0.823399 | 1.41E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene28802 | -0.827105 | 1.88E-54 | -1.69E-46 |
| MS.gene05960 | MS.gene28802 | 0.827461 | 1.54E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene28802 | -0.805787 | 1.13E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene28802 | -0.827946 | 1.18E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene28802 | -0.841567 | 4.40E-58 | -1.69E-46 |
| MS.gene060187 | MS.gene28802 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene06027 | MS.gene28802 | -0.802067 | 6.69E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene28802 | 0.812333 | 4.47E-51 | -1.69E-46 |
| MS.gene06042 | MS.gene28802 | 0.812282 | 4.58E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene28802 | 0.808588 | 2.88E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene28802 | 0.832463 | 9.32E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene28802 | -0.825175 | 5.41E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene28802 | -0.842808 | 2.06E-58 | -1.69E-46 |
| MS.gene060823 | MS.gene28802 | 0.833572 | 4.94E-56 | -1.69E-46 |
| MS.gene06084 | MS.gene28802 | 0.823029 | 1.73E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene28802 | -0.854744 | 1.00E-61 | -1.69E-46 |
| MS.gene061044 | MS.gene28802 | 0.890768 | 7.31E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28802.t1 | MTR_7g109410 | 94.924 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 1.96e-141 | 392 |
| MS.gene28802.t1 | MTR_1g087230 | 56.853 | 197 | 75 | 5 | 1 | 192 | 1 | 192 | 4.54e-72 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28802.t1 | AT4G02090 | 38.579 | 197 | 83 | 9 | 9 | 183 | 16 | 196 | 4.17e-28 | 105 |
Find 39 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTTTCCTCCATCATTTC+TGG | 0.096966 | 7.4:-5978694 | None:intergenic |
| ATAAGCTCTGCTACTGTTAT+TGG | 0.153548 | 7.4:-5978334 | None:intergenic |
| ACCATCATAGTAAATGATTT+TGG | 0.206418 | 7.4:-5978290 | None:intergenic |
| AGAGCTCGATAGATAATTAT+TGG | 0.225759 | 7.4:-5978554 | None:intergenic |
| AACTCAACAACAACATGTTT+TGG | 0.228014 | 7.4:-5978367 | None:intergenic |
| TTGTACCAATCAAACTTCTA+AGG | 0.282032 | 7.4:-5978822 | None:intergenic |
| AATTTGAGTAGGCAATTATC+AGG | 0.284221 | 7.4:+5978727 | MS.gene28802:CDS |
| GCCTAGCTTGGATACTATTA+AGG | 0.302250 | 7.4:+5978762 | MS.gene28802:CDS |
| GCTCTCAAGGCTTTGTTCCT+TGG | 0.316149 | 7.4:+5978572 | MS.gene28802:CDS |
| GCTTTGTTCCTTGGCTTGTA+AGG | 0.325698 | 7.4:+5978581 | MS.gene28802:CDS |
| ACGGGCTTCCCTGGTTTCAT+TGG | 0.343712 | 7.4:-5978472 | None:intergenic |
| ACCAAAATCATTTACTATGA+TGG | 0.388071 | 7.4:+5978289 | MS.gene28802:CDS |
| AAGAATTCGACGACAATCTT+CGG | 0.416240 | 7.4:-5978506 | None:intergenic |
| ATTTCTAGGTTCTTGTCAGC+TGG | 0.436018 | 7.4:-5978427 | None:intergenic |
| TTGTGGCATGAAATGAGATA+TGG | 0.438798 | 7.4:-5978267 | None:intergenic |
| CTTAATCCGACGGGCTTCCC+TGG | 0.445311 | 7.4:-5978481 | None:intergenic |
| AGGTTCTTGTCAGCTGGTAG+TGG | 0.455725 | 7.4:-5978421 | None:intergenic |
| TTGTCAGCTGGTAGTGGTGT+TGG | 0.459537 | 7.4:-5978415 | None:intergenic |
| GTATTCCTTAGAAGTTTGAT+TGG | 0.462601 | 7.4:+5978817 | MS.gene28802:CDS |
| AAGGTTAAGAGAAAGATATC+TGG | 0.480587 | 7.4:+5978790 | MS.gene28802:CDS |
| GGATACTATTAAGGAAAAGA+AGG | 0.488586 | 7.4:+5978771 | MS.gene28802:CDS |
| CAGAGTATTTGAATTTGAGT+AGG | 0.490159 | 7.4:+5978716 | MS.gene28802:CDS |
| GAAAGGGTGGAAGCCTAGCT+TGG | 0.506451 | 7.4:+5978750 | MS.gene28802:CDS |
| TAAGAGAAAGATATCTGGTT+GGG | 0.509583 | 7.4:+5978795 | MS.gene28802:CDS |
| AGTAGGCAATTATCAGGGAA+AGG | 0.530002 | 7.4:+5978733 | MS.gene28802:CDS |
| TGCAGATAAATGAAGAAGAG+AGG | 0.538383 | 7.4:+5978650 | MS.gene28802:CDS |
| TCCTTAATAGTATCCAAGCT+AGG | 0.540949 | 7.4:-5978763 | None:intergenic |
| GGCAATTATCAGGGAAAGGG+TGG | 0.561848 | 7.4:+5978737 | MS.gene28802:CDS |
| AATTATCTATCGAGCTCTCA+AGG | 0.562278 | 7.4:+5978559 | MS.gene28802:CDS |
| AAAGAAAGTTGAAATTGGAG+AGG | 0.565965 | 7.4:+5978615 | MS.gene28802:CDS |
| GTCATGGTTCCAATGAAACC+AGG | 0.568862 | 7.4:+5978463 | MS.gene28802:CDS |
| TTAAGAGAAAGATATCTGGT+TGG | 0.583864 | 7.4:+5978794 | MS.gene28802:CDS |
| ATCTTCGGCACTTAATCCGA+CGG | 0.584491 | 7.4:-5978491 | None:intergenic |
| GTAGGCAATTATCAGGGAAA+GGG | 0.585684 | 7.4:+5978734 | MS.gene28802:CDS |
| ATTAAAAGATGAATTAACGA+TGG | 0.589712 | 7.4:-5978530 | None:intergenic |
| TCTTCGGCACTTAATCCGAC+GGG | 0.598400 | 7.4:-5978490 | None:intergenic |
| ATTTGAGTAGGCAATTATCA+GGG | 0.600835 | 7.4:+5978728 | MS.gene28802:CDS |
| TCATGGTTCCAATGAAACCA+GGG | 0.644756 | 7.4:+5978464 | MS.gene28802:CDS |
| ATGAAACCAGGGAAGCCCGT+CGG | 0.678146 | 7.4:+5978475 | MS.gene28802:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAAGAAAGTTGAAAT+TGG | + | chr7.4:5978610-5978629 | MS.gene28802:CDS | 20.0% |
| !! | AATGAACAAAAAATATGTCA+TGG | + | chr7.4:5978447-5978466 | MS.gene28802:CDS | 20.0% |
| !! | ATTAAAAGATGAATTAACGA+TGG | - | chr7.4:5978533-5978552 | None:intergenic | 20.0% |
| !!! | CATATTTTTTGTTCATTTCT+AGG | - | chr7.4:5978444-5978463 | None:intergenic | 20.0% |
| ! | ACCAAAATCATTTACTATGA+TGG | + | chr7.4:5978289-5978308 | MS.gene28802:CDS | 25.0% |
| !! | ACCATCATAGTAAATGATTT+TGG | - | chr7.4:5978293-5978312 | None:intergenic | 25.0% |
| !!! | ATAGTAAATGATTTTGGTTG+TGG | - | chr7.4:5978287-5978306 | None:intergenic | 25.0% |
| AAAGAAAGTTGAAATTGGAG+AGG | + | chr7.4:5978615-5978634 | MS.gene28802:CDS | 30.0% | |
| AAGGTTAAGAGAAAGATATC+TGG | + | chr7.4:5978790-5978809 | MS.gene28802:CDS | 30.0% | |
| AATTTGAGTAGGCAATTATC+AGG | + | chr7.4:5978727-5978746 | MS.gene28802:CDS | 30.0% | |
| AGAGCTCGATAGATAATTAT+TGG | - | chr7.4:5978557-5978576 | None:intergenic | 30.0% | |
| ATTTGAGTAGGCAATTATCA+GGG | + | chr7.4:5978728-5978747 | MS.gene28802:CDS | 30.0% | |
| GGATACTATTAAGGAAAAGA+AGG | + | chr7.4:5978771-5978790 | MS.gene28802:CDS | 30.0% | |
| GTATTCCTTAGAAGTTTGAT+TGG | + | chr7.4:5978817-5978836 | MS.gene28802:CDS | 30.0% | |
| TAAGAGAAAGATATCTGGTT+GGG | + | chr7.4:5978795-5978814 | MS.gene28802:CDS | 30.0% | |
| TTAAGAGAAAGATATCTGGT+TGG | + | chr7.4:5978794-5978813 | MS.gene28802:CDS | 30.0% | |
| TTGTACCAATCAAACTTCTA+AGG | - | chr7.4:5978825-5978844 | None:intergenic | 30.0% | |
| ! | AACTCAACAACAACATGTTT+TGG | - | chr7.4:5978370-5978389 | None:intergenic | 30.0% |
| ! | ACTCAACAACAACATGTTTT+GGG | - | chr7.4:5978369-5978388 | None:intergenic | 30.0% |
| ! | CAGAGTATTTGAATTTGAGT+AGG | + | chr7.4:5978716-5978735 | MS.gene28802:CDS | 30.0% |
| AAGAATTCGACGACAATCTT+CGG | - | chr7.4:5978509-5978528 | None:intergenic | 35.0% | |
| AATTATCTATCGAGCTCTCA+AGG | + | chr7.4:5978559-5978578 | MS.gene28802:CDS | 35.0% | |
| ATAAGCTCTGCTACTGTTAT+TGG | - | chr7.4:5978337-5978356 | None:intergenic | 35.0% | |
| TCCTTAATAGTATCCAAGCT+AGG | - | chr7.4:5978766-5978785 | None:intergenic | 35.0% | |
| TGCAGATAAATGAAGAAGAG+AGG | + | chr7.4:5978650-5978669 | MS.gene28802:CDS | 35.0% | |
| TTCAAAAACCTTACAAGCCA+AGG | - | chr7.4:5978592-5978611 | None:intergenic | 35.0% | |
| TTGTGGCATGAAATGAGATA+TGG | - | chr7.4:5978270-5978289 | None:intergenic | 35.0% | |
| ! | TGAGTTTTTGCCAGAAATGA+TGG | + | chr7.4:5978684-5978703 | MS.gene28802:CDS | 35.0% |
| AGTAGGCAATTATCAGGGAA+AGG | + | chr7.4:5978733-5978752 | MS.gene28802:CDS | 40.0% | |
| GCCTAGCTTGGATACTATTA+AGG | + | chr7.4:5978762-5978781 | MS.gene28802:CDS | 40.0% | |
| GTAGGCAATTATCAGGGAAA+GGG | + | chr7.4:5978734-5978753 | MS.gene28802:CDS | 40.0% | |
| TCATGGTTCCAATGAAACCA+GGG | + | chr7.4:5978464-5978483 | MS.gene28802:CDS | 40.0% | |
| ! | ATTTCTAGGTTCTTGTCAGC+TGG | - | chr7.4:5978430-5978449 | None:intergenic | 40.0% |
| ! | GTTTTTGCCAGAAATGATGG+AGG | + | chr7.4:5978687-5978706 | MS.gene28802:CDS | 40.0% |
| !!! | GTAGTGGTGTTGGTTTTTTC+TGG | - | chr7.4:5978408-5978427 | None:intergenic | 40.0% |
| ATCTTCGGCACTTAATCCGA+CGG | - | chr7.4:5978494-5978513 | None:intergenic | 45.0% | |
| GCTCTTTCCTCCATCATTTC+TGG | - | chr7.4:5978697-5978716 | None:intergenic | 45.0% | |
| GTCATGGTTCCAATGAAACC+AGG | + | chr7.4:5978463-5978482 | MS.gene28802:CDS | 45.0% | |
| !! | GCTTTGTTCCTTGGCTTGTA+AGG | + | chr7.4:5978581-5978600 | MS.gene28802:CDS | 45.0% |
| AGGTTCTTGTCAGCTGGTAG+TGG | - | chr7.4:5978424-5978443 | None:intergenic | 50.0% | |
| GGCAATTATCAGGGAAAGGG+TGG | + | chr7.4:5978737-5978756 | MS.gene28802:CDS | 50.0% | |
| TCTTCGGCACTTAATCCGAC+GGG | - | chr7.4:5978493-5978512 | None:intergenic | 50.0% | |
| !! | GCTCTCAAGGCTTTGTTCCT+TGG | + | chr7.4:5978572-5978591 | MS.gene28802:CDS | 50.0% |
| !! | TTGTCAGCTGGTAGTGGTGT+TGG | - | chr7.4:5978418-5978437 | None:intergenic | 50.0% |
| ACGGGCTTCCCTGGTTTCAT+TGG | - | chr7.4:5978475-5978494 | None:intergenic | 55.0% | |
| ATGAAACCAGGGAAGCCCGT+CGG | + | chr7.4:5978475-5978494 | MS.gene28802:CDS | 55.0% | |
| GAAAGGGTGGAAGCCTAGCT+TGG | + | chr7.4:5978750-5978769 | MS.gene28802:CDS | 55.0% | |
| ! | CTTAATCCGACGGGCTTCCC+TGG | - | chr7.4:5978484-5978503 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5978259 | 5978852 | 5978259 | ID=MS.gene28802 |
| chr7.4 | mRNA | 5978259 | 5978852 | 5978259 | ID=MS.gene28802.t1;Parent=MS.gene28802 |
| chr7.4 | exon | 5978259 | 5978852 | 5978259 | ID=MS.gene28802.t1.exon1;Parent=MS.gene28802.t1 |
| chr7.4 | CDS | 5978259 | 5978852 | 5978259 | ID=cds.MS.gene28802.t1;Parent=MS.gene28802.t1 |
| Gene Sequence |
| Protein sequence |
