AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30766.t1 MTR_3g073140 98.193 166 3 0 1 166 1 166 1.69e-118 332
MS.gene30766.t1 MTR_4g081120 47.727 132 69 0 21 152 34 165 7.97e-35 120
MS.gene30766.t1 MTR_4g081120 53.000 100 47 0 53 152 46 145 3.27e-34 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30766.t1 AT5G14320 70.513 156 41 2 1 153 1 154 2.62e-76 225
MS.gene30766.t1 AT5G14320 62.069 116 39 2 1 113 1 114 5.03e-44 142
MS.gene30766.t1 AT1G77750 48.387 124 64 0 42 165 27 150 2.09e-37 126

Find 40 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGTCCTGCAATTATTCT+TGG 0.215118 3.1:+59960102 None:intergenic
AATTCAATTCTCTTGTTGTT+TGG 0.264258 3.1:+59960471 None:intergenic
CAGAGGAATAAGGCATATTC+AGG 0.285946 3.1:-59960149 MS.gene30766:CDS
AATTGTGTTACCTTTGGCTT+TGG 0.351843 3.1:+59960600 None:intergenic
GGCTTTGGAGTGTTGAGAAC+TGG 0.358340 3.1:+59960615 None:intergenic
GGTAAGAAGGTTACTGTTGC+TGG 0.372364 3.1:-59960076 MS.gene30766:CDS
AAGAAGAGCTTATTAGTCTC+AGG 0.387619 3.1:-59960353 MS.gene30766:CDS
TTCAGTGTTACAGAGGAATA+AGG 0.388901 3.1:-59960159 MS.gene30766:CDS
ATACATGACTGAAGGAGATT+TGG 0.390225 3.1:-59960316 MS.gene30766:intron
GGAGAAGTGACCATGGCCTT+TGG 0.397280 3.1:+59960653 None:intergenic
GATTCTAACTGATATCAGTA+TGG 0.462554 3.1:-59960403 MS.gene30766:CDS
TTGCAGTACATTCATGGAGT+TGG 0.465952 3.1:-59960444 MS.gene30766:CDS
TATGGAGAATAAAGTTACTA+AGG 0.474154 3.1:-59960385 MS.gene30766:CDS
CAGGACATTGAAGGGTAAGA+AGG 0.478630 3.1:-59960089 MS.gene30766:CDS
TTAATGCACTTAATATAAAG+AGG 0.487114 3.1:-59960192 MS.gene30766:CDS
AGCGTACCAAGAATAATTGC+AGG 0.496459 3.1:-59960108 MS.gene30766:CDS
GAATAATTGCAGGACATTGA+AGG 0.513496 3.1:-59960098 MS.gene30766:CDS
AACTGGGAAGGAAACTCGAT+TGG 0.516558 3.1:+59960632 None:intergenic
TGATTGAATTGTAGGTTCAA+AGG 0.517088 3.1:-59960533 MS.gene30766:intron
ATTGTGTGCGTTCGTGTTGG+TGG 0.534997 3.1:-59960504 MS.gene30766:CDS
GCTTTGGAGTGTTGAGAACT+GGG 0.547804 3.1:+59960616 None:intergenic
AATAATTGCAGGACATTGAA+GGG 0.549157 3.1:-59960097 MS.gene30766:CDS
ATTGAGAGTGAAGGTGCCAC+AGG 0.552065 3.1:+59960681 None:intergenic
ACTTAATATAAAGAGGTTGA+AGG 0.557426 3.1:-59960185 MS.gene30766:CDS
AACATTGTGTGCGTTCGTGT+TGG 0.564683 3.1:-59960507 MS.gene30766:CDS
TCAATCATCTCCAAAGGCCA+TGG 0.572793 3.1:-59960663 MS.gene30766:CDS
GTGCGTTCGTGTTGGTGGTG+TGG 0.576835 3.1:-59960499 MS.gene30766:CDS
TTGGTACGCTGTCCTCTACA+AGG 0.578588 3.1:+59960121 None:intergenic
AGAGGAATAAGGCATATTCA+GGG 0.580912 3.1:-59960148 MS.gene30766:CDS
TGGAGTGTTGAGAACTGGGA+AGG 0.589530 3.1:+59960620 None:intergenic
GTTAACAAATACATGACTGA+AGG 0.596392 3.1:-59960324 MS.gene30766:CDS
TCACTCTCAATCATCTCCAA+AGG 0.619107 3.1:-59960669 MS.gene30766:CDS
ATTCAGGGACTTCCTTGTAG+AGG 0.621863 3.1:-59960133 MS.gene30766:CDS
AGTACATTCATGGAGTTGGA+AGG 0.622828 3.1:-59960440 MS.gene30766:CDS
AGAAGAGCTTATTAGTCTCA+GGG 0.625713 3.1:-59960352 MS.gene30766:CDS
AAGGAGATTCAGTGTTACAG+AGG 0.641827 3.1:-59960166 MS.gene30766:CDS
TCTCAACACTCCAAAGCCAA+AGG 0.652992 3.1:-59960610 MS.gene30766:intron
CTCGATTGGAGAAGTGACCA+TGG 0.660409 3.1:+59960646 None:intergenic
TTGGAGATGATTGAGAGTGA+AGG 0.661623 3.1:+59960672 None:intergenic
ACAAACGCTTTCAATGCCTG+TGG 0.693733 3.1:-59960697 MS.gene30766:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTAATTGTAATTGTAA+TGG - chr3.1:59960517-59960536 MS.gene30766:CDS 15.0%
!! TTAATGCACTTAATATAAAG+AGG - chr3.1:59960570-59960589 MS.gene30766:intron 20.0%
! ACTTAATATAAAGAGGTTGA+AGG - chr3.1:59960577-59960596 MS.gene30766:intron 25.0%
! TATGGAGAATAAAGTTACTA+AGG - chr3.1:59960377-59960396 MS.gene30766:CDS 25.0%
!!! AATTCAATTCTCTTGTTGTT+TGG + chr3.1:59960294-59960313 None:intergenic 25.0%
AATAATTGCAGGACATTGAA+GGG - chr3.1:59960665-59960684 MS.gene30766:CDS 30.0%
ATCAGAATCAGAATGGAATT+TGG + chr3.1:59960213-59960232 None:intergenic 30.0%
CAATTCAATCAGAATCAGAA+TGG + chr3.1:59960220-59960239 None:intergenic 30.0%
GATTCTAACTGATATCAGTA+TGG - chr3.1:59960359-59960378 MS.gene30766:CDS 30.0%
GTTAACAAATACATGACTGA+AGG - chr3.1:59960438-59960457 MS.gene30766:CDS 30.0%
TCTGATTCTGATTGAATTGT+AGG - chr3.1:59960221-59960240 MS.gene30766:intron 30.0%
TGATTGAATTGTAGGTTCAA+AGG - chr3.1:59960229-59960248 MS.gene30766:intron 30.0%
TTGTTTGTGAATTTCAGAGA+AGG - chr3.1:59960546-59960565 MS.gene30766:intron 30.0%
AACCCTAATTGTGTTACCTT+TGG + chr3.1:59960171-59960190 None:intergenic 35.0%
AAGCCAAAGGTAACACAATT+AGG - chr3.1:59960165-59960184 MS.gene30766:CDS 35.0%
AGAGGAATAAGGCATATTCA+GGG - chr3.1:59960614-59960633 MS.gene30766:CDS 35.0%
AGCCAAAGGTAACACAATTA+GGG - chr3.1:59960166-59960185 MS.gene30766:CDS 35.0%
ATACATGACTGAAGGAGATT+TGG - chr3.1:59960446-59960465 MS.gene30766:CDS 35.0%
CAATGTCCTGCAATTATTCT+TGG + chr3.1:59960663-59960682 None:intergenic 35.0%
GAATAATTGCAGGACATTGA+AGG - chr3.1:59960664-59960683 MS.gene30766:CDS 35.0%
TTCAGTGTTACAGAGGAATA+AGG - chr3.1:59960603-59960622 MS.gene30766:intron 35.0%
! AAGAAGAGCTTATTAGTCTC+AGG - chr3.1:59960409-59960428 MS.gene30766:CDS 35.0%
! AATTGTGTTACCTTTGGCTT+TGG + chr3.1:59960165-59960184 None:intergenic 35.0%
! AGAAGAGCTTATTAGTCTCA+GGG - chr3.1:59960410-59960429 MS.gene30766:CDS 35.0%
! TTTTCGTTGCAGTACATTCA+TGG - chr3.1:59960312-59960331 MS.gene30766:intron 35.0%
AAGGAGATTCAGTGTTACAG+AGG - chr3.1:59960596-59960615 MS.gene30766:intron 40.0%
AGCGTACCAAGAATAATTGC+AGG - chr3.1:59960654-59960673 MS.gene30766:CDS 40.0%
AGTACATTCATGGAGTTGGA+AGG - chr3.1:59960322-59960341 MS.gene30766:CDS 40.0%
CAGAGGAATAAGGCATATTC+AGG - chr3.1:59960613-59960632 MS.gene30766:CDS 40.0%
TCACTCTCAATCATCTCCAA+AGG - chr3.1:59960093-59960112 MS.gene30766:CDS 40.0%
TTGCAGTACATTCATGGAGT+TGG - chr3.1:59960318-59960337 MS.gene30766:CDS 40.0%
TTGGAGATGATTGAGAGTGA+AGG + chr3.1:59960093-59960112 None:intergenic 40.0%
AACATTGTGTGCGTTCGTGT+TGG - chr3.1:59960255-59960274 MS.gene30766:intron 45.0%
AACTGGGAAGGAAACTCGAT+TGG + chr3.1:59960133-59960152 None:intergenic 45.0%
ACAAACGCTTTCAATGCCTG+TGG - chr3.1:59960065-59960084 MS.gene30766:CDS 45.0%
ATTCAGGGACTTCCTTGTAG+AGG - chr3.1:59960629-59960648 MS.gene30766:CDS 45.0%
CAGGACATTGAAGGGTAAGA+AGG - chr3.1:59960673-59960692 MS.gene30766:CDS 45.0%
GGTAAGAAGGTTACTGTTGC+TGG - chr3.1:59960686-59960705 MS.gene30766:CDS 45.0%
TCAATCATCTCCAAAGGCCA+TGG - chr3.1:59960099-59960118 MS.gene30766:CDS 45.0%
TCTCAACACTCCAAAGCCAA+AGG - chr3.1:59960152-59960171 MS.gene30766:CDS 45.0%
!! GCTTTGGAGTGTTGAGAACT+GGG + chr3.1:59960149-59960168 None:intergenic 45.0%
ATTGTGTGCGTTCGTGTTGG+TGG - chr3.1:59960258-59960277 MS.gene30766:intron 50.0%
CTCGATTGGAGAAGTGACCA+TGG + chr3.1:59960119-59960138 None:intergenic 50.0%
TTGGTACGCTGTCCTCTACA+AGG + chr3.1:59960644-59960663 None:intergenic 50.0%
!! ATTGAGAGTGAAGGTGCCAC+AGG + chr3.1:59960084-59960103 None:intergenic 50.0%
!! GGCTTTGGAGTGTTGAGAAC+TGG + chr3.1:59960150-59960169 None:intergenic 50.0%
!! TGGAGTGTTGAGAACTGGGA+AGG + chr3.1:59960145-59960164 None:intergenic 50.0%
GGAGAAGTGACCATGGCCTT+TGG + chr3.1:59960112-59960131 None:intergenic 55.0%
!! GTGCGTTCGTGTTGGTGGTG+TGG - chr3.1:59960263-59960282 MS.gene30766:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 59960060 59960724 59960060 ID=MS.gene30766
chr3.1 mRNA 59960060 59960724 59960060 ID=MS.gene30766.t1;Parent=MS.gene30766
chr3.1 exon 59960611 59960724 59960611 ID=MS.gene30766.t1.exon1;Parent=MS.gene30766.t1
chr3.1 CDS 59960611 59960724 59960611 ID=cds.MS.gene30766.t1;Parent=MS.gene30766.t1
chr3.1 exon 59960317 59960541 59960317 ID=MS.gene30766.t1.exon2;Parent=MS.gene30766.t1
chr3.1 CDS 59960317 59960541 59960317 ID=cds.MS.gene30766.t1;Parent=MS.gene30766.t1
chr3.1 exon 59960060 59960221 59960060 ID=MS.gene30766.t1.exon3;Parent=MS.gene30766.t1
chr3.1 CDS 59960060 59960221 59960060 ID=cds.MS.gene30766.t1;Parent=MS.gene30766.t1
Gene Sequence

>MS.gene30766

ATGGTACAAACGCTTTCAATGCCTGTGGCACCTTCACTCTCAATCATCTCCAAAGGCCATGGTCACTTCTCCAATCGAGTTTCCTTCCCAGTTCTCAACACTCCAAAGCCAAAGGTAACACAATTAGGGTTTCAAAATTCAACATAAATTCCAAATTCCATTCTGATTCTGATTGAATTGTAGGTTCAAAGGCTAAACATTGTGTGCGTTCGTGTTGGTGGTGTGGAAATTCCAAACAACAAGAGAATTGAATTTTCGTTGCAGTACATTCATGGAGTTGGAAGGAACAGAGCTAGACAGATTCTAACTGATATCAGTATGGAGAATAAAGTTACTAAGGAATTGAGTGAAGAAGAGCTTATTAGTCTCAGGGATGAAGTTAACAAATACATGACTGAAGGAGATTTGGTAAAAAAAAAATTAAAAATCTCATTTTTTTTTGTTTTCTATTATTTTGTTTTGTAATTGTAATTGTAATGGTTTGATTTGTTTGTGAATTTCAGAGAAGGTTTAATGCACTTAATATAAAGAGGTTGAAGGAGATTCAGTGTTACAGAGGAATAAGGCATATTCAGGGACTTCCTTGTAGAGGACAGCGTACCAAGAATAATTGCAGGACATTGAAGGGTAAGAAGGTTACTGTTGCTGGCAAGAAGAAAAAGTAA

Protein sequence

>MS.gene30766.t1

MVQTLSMPVAPSLSIISKGHGHFSNRVSFPVLNTPKPKVQRLNIVCVRVGGVEIPNNKRIEFSLQYIHGVGRNRARQILTDISMENKVTKELSEEELISLRDEVNKYMTEGDLRRFNALNIKRLKEIQCYRGIRHIQGLPCRGQRTKNNCRTLKGKKVTVAGKKKK