Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34831.t1 | XP_003595751.1 | 97.1 | 548 | 15 | 1 | 1 | 548 | 1 | 547 | 2.7e-309 | 1070.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34831.t1 | Q6YPG5 | 64.8 | 554 | 175 | 4 | 3 | 548 | 6 | 547 | 2.9e-203 | 709.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34831.t1 | G7IGS4 | 97.1 | 548 | 15 | 1 | 1 | 548 | 1 | 547 | 2.0e-309 | 1070.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene34831 | 0.832217 | 1.07E-55 | -1.69E-46 |
| MS.gene049351 | MS.gene34831 | 0.832917 | 7.19E-56 | -1.69E-46 |
| MS.gene049356 | MS.gene34831 | 0.806783 | 6.96E-50 | -1.69E-46 |
| MS.gene049389 | MS.gene34831 | 0.841377 | 4.93E-58 | -1.69E-46 |
| MS.gene049427 | MS.gene34831 | 0.873305 | 1.63E-67 | -1.69E-46 |
| MS.gene049491 | MS.gene34831 | 0.877232 | 7.41E-69 | -1.69E-46 |
| MS.gene049614 | MS.gene34831 | 0.809943 | 1.47E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene34831 | 0.815523 | 8.85E-52 | -1.69E-46 |
| MS.gene049662 | MS.gene34831 | 0.81687 | 4.43E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene34831 | 0.858142 | 1.01E-62 | -1.69E-46 |
| MS.gene049760 | MS.gene34831 | 0.805041 | 1.62E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene34831 | 0.85087 | 1.28E-60 | -1.69E-46 |
| MS.gene050099 | MS.gene34831 | 0.832266 | 1.04E-55 | -1.69E-46 |
| MS.gene050128 | MS.gene34831 | 0.832419 | 9.56E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene34831 | 0.909202 | 7.39E-82 | -1.69E-46 |
| MS.gene050397 | MS.gene34831 | 0.810234 | 1.28E-50 | -1.69E-46 |
| MS.gene050404 | MS.gene34831 | 0.80938 | 1.95E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene34831 | 0.847461 | 1.14E-59 | -1.69E-46 |
| MS.gene050529 | MS.gene34831 | 0.831446 | 1.66E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene34831 | 0.815391 | 9.47E-52 | -1.69E-46 |
| MS.gene050767 | MS.gene34831 | 0.826362 | 2.83E-54 | -1.69E-46 |
| MS.gene050770 | MS.gene34831 | 0.828573 | 8.34E-55 | -1.69E-46 |
| MS.gene050783 | MS.gene34831 | 0.820918 | 5.32E-53 | -1.69E-46 |
| MS.gene050812 | MS.gene34831 | 0.874911 | 4.66E-68 | -1.69E-46 |
| MS.gene05101 | MS.gene34831 | 0.887518 | 1.33E-72 | -1.69E-46 |
| MS.gene051387 | MS.gene34831 | 0.800082 | 1.70E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene34831 | 0.886735 | 2.64E-72 | -1.69E-46 |
| MS.gene051582 | MS.gene34831 | 0.837506 | 4.99E-57 | -1.69E-46 |
| MS.gene051662 | MS.gene34831 | 0.810257 | 1.26E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene34831 | 0.842854 | 2.01E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene34831 | 0.855771 | 5.05E-62 | -1.69E-46 |
| MS.gene052178 | MS.gene34831 | 0.836193 | 1.08E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene34831 | 0.895039 | 1.40E-75 | -1.69E-46 |
| MS.gene052253 | MS.gene34831 | 0.823672 | 1.22E-53 | -1.69E-46 |
| MS.gene052290 | MS.gene34831 | 0.831635 | 1.49E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene34831 | 0.874827 | 4.98E-68 | -1.69E-46 |
| MS.gene052326 | MS.gene34831 | 0.891706 | 3.11E-74 | -1.69E-46 |
| MS.gene052340 | MS.gene34831 | 0.850998 | 1.18E-60 | -1.69E-46 |
| MS.gene052485 | MS.gene34831 | 0.835886 | 1.29E-56 | -1.69E-46 |
| MS.gene053107 | MS.gene34831 | 0.804467 | 2.13E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene34831 | 0.818754 | 1.66E-52 | -1.69E-46 |
| MS.gene053322 | MS.gene34831 | 0.819134 | 1.36E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene34831 | 0.849913 | 2.38E-60 | -1.69E-46 |
| MS.gene053559 | MS.gene34831 | 0.841907 | 3.57E-58 | -1.69E-46 |
| MS.gene053836 | MS.gene34831 | 0.87719 | 7.67E-69 | -1.69E-46 |
| MS.gene053907 | MS.gene34831 | 0.81888 | 1.56E-52 | -1.69E-46 |
| MS.gene054123 | MS.gene34831 | 0.891256 | 4.69E-74 | -1.69E-46 |
| MS.gene054376 | MS.gene34831 | 0.844647 | 6.64E-59 | -1.69E-46 |
| MS.gene05455 | MS.gene34831 | 0.860144 | 2.54E-63 | -1.69E-46 |
| MS.gene054802 | MS.gene34831 | 0.826079 | 3.30E-54 | -1.69E-46 |
| MS.gene054803 | MS.gene34831 | 0.829869 | 4.04E-55 | -1.69E-46 |
| MS.gene054804 | MS.gene34831 | 0.829083 | 6.28E-55 | -1.69E-46 |
| MS.gene054930 | MS.gene34831 | 0.804789 | 1.82E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene34831 | 0.828812 | 7.30E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene34831 | 0.800319 | 1.52E-48 | -1.69E-46 |
| MS.gene055277 | MS.gene34831 | 0.872423 | 3.22E-67 | -1.69E-46 |
| MS.gene055390 | MS.gene34831 | 0.886873 | 2.34E-72 | -1.69E-46 |
| MS.gene055397 | MS.gene34831 | 0.840092 | 1.07E-57 | -1.69E-46 |
| MS.gene055412 | MS.gene34831 | 0.846577 | 1.99E-59 | -1.69E-46 |
| MS.gene055422 | MS.gene34831 | 0.882658 | 8.66E-71 | -1.69E-46 |
| MS.gene055428 | MS.gene34831 | 0.868714 | 5.32E-66 | -1.69E-46 |
| MS.gene055499 | MS.gene34831 | 0.804579 | 2.02E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene34831 | 0.806096 | 9.71E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene34831 | 0.810252 | 1.26E-50 | -1.69E-46 |
| MS.gene055896 | MS.gene34831 | 0.839067 | 1.98E-57 | -1.69E-46 |
| MS.gene055930 | MS.gene34831 | 0.809237 | 2.09E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene34831 | 0.842467 | 2.54E-58 | -1.69E-46 |
| MS.gene056410 | MS.gene34831 | 0.82125 | 4.46E-53 | -1.69E-46 |
| MS.gene05647 | MS.gene34831 | 0.806045 | 9.95E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene34831 | 0.870783 | 1.12E-66 | -1.69E-46 |
| MS.gene056612 | MS.gene34831 | 0.861229 | 1.19E-63 | -1.69E-46 |
| MS.gene056659 | MS.gene34831 | 0.847701 | 9.79E-60 | -1.69E-46 |
| MS.gene056719 | MS.gene34831 | 0.804075 | 2.57E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene34831 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene056897 | MS.gene34831 | 0.80676 | 7.03E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene34831 | 0.835511 | 1.61E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene34831 | 0.856589 | 2.90E-62 | -1.69E-46 |
| MS.gene057121 | MS.gene34831 | 0.857597 | 1.46E-62 | -1.69E-46 |
| MS.gene057123 | MS.gene34831 | 0.804354 | 2.25E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene34831 | 0.805897 | 1.07E-49 | -1.69E-46 |
| MS.gene057146 | MS.gene34831 | 0.823594 | 1.27E-53 | -1.69E-46 |
| MS.gene057245 | MS.gene34831 | 0.808412 | 3.14E-50 | -1.69E-46 |
| MS.gene057439 | MS.gene34831 | 0.80103 | 1.09E-48 | -1.69E-46 |
| MS.gene057448 | MS.gene34831 | 0.832163 | 1.11E-55 | -1.69E-46 |
| MS.gene057449 | MS.gene34831 | 0.838176 | 3.36E-57 | -1.69E-46 |
| MS.gene057450 | MS.gene34831 | 0.843945 | 1.03E-58 | -1.69E-46 |
| MS.gene058002 | MS.gene34831 | 0.889791 | 1.77E-73 | -1.69E-46 |
| MS.gene058082 | MS.gene34831 | 0.838157 | 3.40E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene34831 | 0.858512 | 7.83E-63 | -1.69E-46 |
| MS.gene058142 | MS.gene34831 | 0.848685 | 5.24E-60 | -1.69E-46 |
| MS.gene058233 | MS.gene34831 | 0.842693 | 2.21E-58 | -1.69E-46 |
| MS.gene058276 | MS.gene34831 | 0.831723 | 1.42E-55 | -1.69E-46 |
| MS.gene058293 | MS.gene34831 | 0.853534 | 2.24E-61 | -1.69E-46 |
| MS.gene058365 | MS.gene34831 | 0.873763 | 1.14E-67 | -1.69E-46 |
| MS.gene058660 | MS.gene34831 | 0.906027 | 2.30E-80 | -1.69E-46 |
| MS.gene058787 | MS.gene34831 | 0.805456 | 1.32E-49 | -1.69E-46 |
| MS.gene058845 | MS.gene34831 | 0.82787 | 1.23E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene34831 | 0.807743 | 4.36E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene34831 | 0.818856 | 1.58E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene34831 | 0.868318 | 7.14E-66 | -1.69E-46 |
| MS.gene059342 | MS.gene34831 | 0.82505 | 5.79E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene34831 | 0.842754 | 2.13E-58 | -1.69E-46 |
| MS.gene059536 | MS.gene34831 | 0.812312 | 4.52E-51 | -1.69E-46 |
| MS.gene059597 | MS.gene34831 | 0.896182 | 4.72E-76 | -1.69E-46 |
| MS.gene05959 | MS.gene34831 | 0.871028 | 9.34E-67 | -1.69E-46 |
| MS.gene059676 | MS.gene34831 | 0.876845 | 1.01E-68 | -1.69E-46 |
| MS.gene059688 | MS.gene34831 | 0.869665 | 2.61E-66 | -1.69E-46 |
| MS.gene059692 | MS.gene34831 | 0.87962 | 1.07E-69 | -1.69E-46 |
| MS.gene059695 | MS.gene34831 | 0.879069 | 1.68E-69 | -1.69E-46 |
| MS.gene059708 | MS.gene34831 | 0.866913 | 2.01E-65 | -1.69E-46 |
| MS.gene05984 | MS.gene34831 | 0.840252 | 9.73E-58 | -1.69E-46 |
| MS.gene059894 | MS.gene34831 | 0.873405 | 1.51E-67 | -1.69E-46 |
| MS.gene059899 | MS.gene34831 | 0.818458 | 1.94E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene34831 | 0.850598 | 1.53E-60 | -1.69E-46 |
| MS.gene060005 | MS.gene34831 | 0.808508 | 2.99E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene34831 | 0.820868 | 5.47E-53 | -1.69E-46 |
| MS.gene060407 | MS.gene34831 | 0.856662 | 2.76E-62 | -1.69E-46 |
| MS.gene060495 | MS.gene34831 | 0.815059 | 1.12E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene34831 | 0.818577 | 1.82E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene34831 | 0.800159 | 1.64E-48 | -1.69E-46 |
| MS.gene06090 | MS.gene34831 | 0.834685 | 2.60E-56 | -1.69E-46 |
| MS.gene060971 | MS.gene34831 | 0.800236 | 1.58E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene34831 | 0.864093 | 1.56E-64 | -1.69E-46 |
| MS.gene061051 | MS.gene34831 | -0.858365 | 8.66E-63 | -1.69E-46 |
| MS.gene061112 | MS.gene34831 | 0.807191 | 5.70E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene34831 | 0.805365 | 1.38E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene34831 | 0.830879 | 2.29E-55 | -1.69E-46 |
| MS.gene061265 | MS.gene34831 | 0.814322 | 1.63E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34831.t1 | MTR_2g060420 | 97.080 | 548 | 15 | 1 | 1 | 548 | 1 | 547 | 0.0 | 1095 |
| MS.gene34831.t1 | MTR_2g060420 | 96.287 | 404 | 14 | 1 | 1 | 404 | 1 | 403 | 0.0 | 789 |
| MS.gene34831.t1 | MTR_2g060420 | 96.750 | 400 | 12 | 1 | 1 | 400 | 1 | 399 | 0.0 | 788 |
| MS.gene34831.t1 | MTR_2g060420 | 96.694 | 363 | 11 | 1 | 1 | 363 | 1 | 362 | 0.0 | 714 |
| MS.gene34831.t1 | MTR_4g074870 | 24.359 | 468 | 240 | 14 | 86 | 481 | 82 | 507 | 7.37e-25 | 108 |
| MS.gene34831.t1 | MTR_7g090640 | 26.911 | 327 | 194 | 8 | 203 | 525 | 298 | 583 | 6.87e-24 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34831.t1 | AT3G47450 | 63.509 | 570 | 177 | 7 | 1 | 548 | 1 | 561 | 0.0 | 696 |
| MS.gene34831.t1 | AT3G47450 | 63.158 | 570 | 179 | 7 | 1 | 548 | 1 | 561 | 0.0 | 692 |
| MS.gene34831.t1 | AT4G10620 | 23.387 | 496 | 275 | 12 | 86 | 520 | 79 | 530 | 2.84e-25 | 110 |
Find 145 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAACCTTCTTCTCTTTC+TGG | 0.093378 | 4.4:-61164287 | None:intergenic |
| GTGTGAATTTGAGGGAATTT+GGG | 0.193149 | 4.4:-61161294 | None:intergenic |
| TTAGTCACAACCATTATTAT+TGG | 0.202467 | 4.4:-61163741 | None:intergenic |
| ATTCAAATTAATGCTTTCTT+TGG | 0.211740 | 4.4:+61167077 | MS.gene34831:CDS |
| GGTGTGAATTTGAGGGAATT+TGG | 0.249809 | 4.4:-61161295 | None:intergenic |
| CAAAGCTCTAATTGTCAAAT+TGG | 0.252827 | 4.4:+61162090 | MS.gene34831:CDS |
| TAAAATGTTAAACATGTTTC+CGG | 0.262455 | 4.4:-61167612 | None:intergenic |
| GGAATTCAAAAGTACCATTT+TGG | 0.269489 | 4.4:-61168978 | None:intergenic |
| AAAGGTGCTTCGACAGTAAA+TGG | 0.298977 | 4.4:+61167423 | MS.gene34831:CDS |
| TAGATATAACTTACTTAATA+AGG | 0.304884 | 4.4:-61165538 | None:intergenic |
| GCTGTTCCTGGAACTACCTT+AGG | 0.306684 | 4.4:+61167050 | MS.gene34831:CDS |
| AGCACCTTTGTTTGTATTGT+CGG | 0.326011 | 4.4:-61167409 | None:intergenic |
| ATCTTAATTGTGTTGGGGAT+TGG | 0.326100 | 4.4:+61163878 | MS.gene34831:CDS |
| AAGAAAGCATTAATTTGAAT+TGG | 0.326794 | 4.4:-61167074 | None:intergenic |
| GCCTTCTGGTAAGCGAGATT+TGG | 0.327081 | 4.4:-61161355 | None:intergenic |
| TTTGAGGGAATTTGGGGTTT+GGG | 0.332989 | 4.4:-61161287 | None:intergenic |
| CCAGTACAGTCTGCTGTTCC+TGG | 0.333865 | 4.4:+61167038 | MS.gene34831:CDS |
| AAATCATTGGTAGGAATAAC+TGG | 0.335779 | 4.4:+61164249 | MS.gene34831:CDS |
| ATATGAATTGGCATCCTCTT+AGG | 0.337943 | 4.4:-61167639 | None:intergenic |
| AATAATAATGGTTGTGACTA+AGG | 0.353009 | 4.4:+61163743 | MS.gene34831:CDS |
| TACTGTTGTTGTTCTGTTTG+AGG | 0.355375 | 4.4:-61161452 | None:intergenic |
| TCTTAATTGTGTTGGGGATT+GGG | 0.360762 | 4.4:+61163879 | MS.gene34831:CDS |
| ACAAACCAATTCATAGGTTT+CGG | 0.367199 | 4.4:-61161580 | None:intergenic |
| CAAATTAATGCTTTCTTTGG+AGG | 0.367521 | 4.4:+61167080 | MS.gene34831:CDS |
| CAAGGTTTGGTGTGAATTTG+AGG | 0.371755 | 4.4:-61161303 | None:intergenic |
| ATACTGATCTTAATTGTGTT+GGG | 0.374200 | 4.4:+61163872 | MS.gene34831:CDS |
| CCTCTCCGAAACCTATGAAT+TGG | 0.374687 | 4.4:+61161575 | MS.gene34831:CDS |
| CCATTACCAGTAGGCAAGGC+TGG | 0.375446 | 4.4:+61168903 | MS.gene34831:CDS |
| GGGAAGAGATGTTTACATTC+TGG | 0.376268 | 4.4:+61164889 | MS.gene34831:intron |
| AGAGATACAAACCAATTCAT+AGG | 0.380236 | 4.4:-61161586 | None:intergenic |
| CAATTTGACAATTAGAGCTT+TGG | 0.384138 | 4.4:-61162089 | None:intergenic |
| GCAGCGGCTCCGGTGCCTTC+TGG | 0.386212 | 4.4:-61161369 | None:intergenic |
| CAAATGACTGCTGGCGTTCG+AGG | 0.395487 | 4.4:-61161413 | None:intergenic |
| ACTGAGGGGCCTATCTTTCC+CGG | 0.397514 | 4.4:+61167284 | MS.gene34831:CDS |
| TGTTAGAATTGATGTCTTGA+AGG | 0.398535 | 4.4:+61167479 | MS.gene34831:CDS |
| ATTGGCCCTAAGGTAGTTCC+AGG | 0.402457 | 4.4:-61167056 | None:intergenic |
| GTTGATATTGTTGACTTCAA+TGG | 0.403407 | 4.4:+61163678 | MS.gene34831:CDS |
| ATTTGAGGGAATTTGGGGTT+TGG | 0.411175 | 4.4:-61161288 | None:intergenic |
| GAAAAGAGAAGGCTGAGAAC+TGG | 0.412977 | 4.4:+61168235 | MS.gene34831:CDS |
| CCAGCCTTGCCTACTGGTAA+TGG | 0.414914 | 4.4:-61168903 | None:intergenic |
| TCTGTGGGCACCATATGAAT+TGG | 0.416048 | 4.4:-61167651 | None:intergenic |
| CTTTGTGCAGCTGCAGCCAC+TGG | 0.422464 | 4.4:-61167008 | None:intergenic |
| GATACTGATCTTAATTGTGT+TGG | 0.426541 | 4.4:+61163871 | MS.gene34831:CDS |
| TCTCACAAGTTCAAAATCAT+TGG | 0.436735 | 4.4:+61164236 | MS.gene34831:CDS |
| TTGGGGTTTGGGAGAGAAAG+AGG | 0.439929 | 4.4:-61161276 | None:intergenic |
| AGAAAGAGGTGTTAAGAAAG+TGG | 0.442252 | 4.4:-61161262 | None:intergenic |
| ATTGCCGTCGCTTCTTGCTA+TGG | 0.444932 | 4.4:+61161504 | MS.gene34831:CDS |
| TGGTGACGGAGCAGCGGCTC+CGG | 0.447078 | 4.4:-61161379 | None:intergenic |
| AGCAGGAAATGAGACCAAAA+TGG | 0.452527 | 4.4:+61168964 | MS.gene34831:CDS |
| GCTTGCGATATTGCTATATC+AGG | 0.456295 | 4.4:+61168750 | MS.gene34831:CDS |
| GTTCTGTTTGAGGATGAGAT+TGG | 0.457626 | 4.4:-61161442 | None:intergenic |
| TGGTGCTAATCCAATAATAA+TGG | 0.459624 | 4.4:+61163731 | MS.gene34831:CDS |
| AAGAACATACCGGGAAAGAT+AGG | 0.460823 | 4.4:-61167293 | None:intergenic |
| TCTGCCCCAGCCTTGCCTAC+TGG | 0.461104 | 4.4:-61168909 | None:intergenic |
| GCAGAGAATGAGACAAGGTT+TGG | 0.467850 | 4.4:-61161316 | None:intergenic |
| AAATCCAGGAGCGTCATTAT+CGG | 0.470024 | 4.4:-61161550 | None:intergenic |
| AATTCATCTGCTTTCTCTGT+GGG | 0.471683 | 4.4:-61167666 | None:intergenic |
| CTGTTCCTGGAACTACCTTA+GGG | 0.475173 | 4.4:+61167051 | MS.gene34831:CDS |
| TGTTGGGGATTGGGTTGTAG+AGG | 0.476151 | 4.4:+61163888 | MS.gene34831:CDS |
| AAAATATTGAAAATCCATTC+AGG | 0.478937 | 4.4:-61167446 | None:intergenic |
| TAAGAGGATGCCAATTCATA+TGG | 0.485520 | 4.4:+61167641 | MS.gene34831:CDS |
| TTATTGTCACAGAAAGAACT+TGG | 0.486062 | 4.4:+61168189 | MS.gene34831:intron |
| AAGGTTTGGTGTGAATTTGA+GGG | 0.486640 | 4.4:-61161302 | None:intergenic |
| CAATTAAGATCAGTATCTCT+TGG | 0.489229 | 4.4:-61163865 | None:intergenic |
| TGGTTGCTTCAAATGACTGC+TGG | 0.489415 | 4.4:-61161422 | None:intergenic |
| AGGTGTTAAGAAAGTGGATA+GGG | 0.492139 | 4.4:-61161256 | None:intergenic |
| ATAACTGCTGTTGGAGGACA+TGG | 0.492972 | 4.4:+61161995 | MS.gene34831:CDS |
| ATTAATGCTTTCTTTGGAGG+AGG | 0.494082 | 4.4:+61167083 | MS.gene34831:CDS |
| CATTACCAGTAGGCAAGGCT+GGG | 0.495878 | 4.4:+61168904 | MS.gene34831:CDS |
| CCAGGAACAGCAGACTGTAC+TGG | 0.496203 | 4.4:-61167038 | None:intergenic |
| CGTTCGAGGAACTTCTCGCC+TGG | 0.498839 | 4.4:-61161399 | None:intergenic |
| AGAAATCCAGAAAGAGAAGA+AGG | 0.499062 | 4.4:+61164281 | MS.gene34831:CDS |
| AGCAAGGGAGGATAGATCTT+CGG | 0.499291 | 4.4:-61167250 | None:intergenic |
| GTCGCTTCTTGCTATGGCTG+TGG | 0.500619 | 4.4:+61161510 | MS.gene34831:CDS |
| CTGTGGTGGAGATGAACTCC+TGG | 0.504637 | 4.4:-61167207 | None:intergenic |
| TCGACAGTAAATGGCCTGAA+TGG | 0.506669 | 4.4:+61167432 | MS.gene34831:CDS |
| GATATTGCTATATCAGGTCT+AGG | 0.507163 | 4.4:+61168756 | MS.gene34831:CDS |
| TGCTCCTAAAAGCCGACTGA+GGG | 0.509262 | 4.4:+61167269 | MS.gene34831:CDS |
| TCCCGAGTGAGAGATCTTGC+TGG | 0.512842 | 4.4:+61163711 | MS.gene34831:CDS |
| TAATGCTTTCTTTGGAGGAG+GGG | 0.513509 | 4.4:+61167085 | MS.gene34831:CDS |
| ATTAATTTGAATTGGCCCTA+AGG | 0.514252 | 4.4:-61167066 | None:intergenic |
| AGGATGGCTTTCTGTTGAGC+CGG | 0.515806 | 4.4:+61168776 | MS.gene34831:CDS |
| AGGAACTTCTCGCCTGGTGA+CGG | 0.516252 | 4.4:-61161393 | None:intergenic |
| GAGGTGTTAAGAAAGTGGAT+AGG | 0.522644 | 4.4:-61161257 | None:intergenic |
| ACAAAATGCAATAGACACTA+CGG | 0.525515 | 4.4:+61168824 | MS.gene34831:CDS |
| TAGGCAAGGCTGGGGCAGAA+TGG | 0.535765 | 4.4:+61168913 | MS.gene34831:CDS |
| TACTGATCTTAATTGTGTTG+GGG | 0.536356 | 4.4:+61163873 | MS.gene34831:CDS |
| CACCAGCAAGATCTCTCACT+CGG | 0.537176 | 4.4:-61163713 | None:intergenic |
| CAAAATGCAATAGACACTAC+GGG | 0.543069 | 4.4:+61168825 | MS.gene34831:CDS |
| TTACAGGGTTCAGCTAATGT+TGG | 0.543821 | 4.4:+61165495 | MS.gene34831:intron |
| GGAGCCGCTGCTCCGTCACC+AGG | 0.550022 | 4.4:+61161381 | MS.gene34831:CDS |
| TTAATGCTTTCTTTGGAGGA+GGG | 0.553702 | 4.4:+61167084 | MS.gene34831:CDS |
| CAGCTTAAAACTGTTCTATG+TGG | 0.554450 | 4.4:+61161941 | MS.gene34831:CDS |
| AATCTGTGTGACCTACCAAC+CGG | 0.555680 | 4.4:-61168795 | None:intergenic |
| TGTGAATTTGAGGGAATTTG+GGG | 0.556857 | 4.4:-61161293 | None:intergenic |
| AGCTTAAAACTGTTCTATGT+GGG | 0.557452 | 4.4:+61161942 | MS.gene34831:CDS |
| TTTCTGTTGAGCCGGTTGGT+AGG | 0.558950 | 4.4:+61168784 | MS.gene34831:CDS |
| ACTTCCGATAATGACGCTCC+TGG | 0.559301 | 4.4:+61161546 | MS.gene34831:CDS |
| TGGCTTTCTGTTGAGCCGGT+TGG | 0.564204 | 4.4:+61168780 | MS.gene34831:CDS |
| GGAGGACATGGAGGATACTC+TGG | 0.566422 | 4.4:+61162007 | MS.gene34831:CDS |
| TCATTATCGGAAGTTTGTAA+AGG | 0.566778 | 4.4:-61161537 | None:intergenic |
| ACAAGTTCAAAATCATTGGT+AGG | 0.566810 | 4.4:+61164240 | MS.gene34831:CDS |
| AACAGGTTTGGGGACATGTA+CGG | 0.568281 | 4.4:-61168862 | None:intergenic |
| ATAGCAAGAAGCGACGGCAA+TGG | 0.571002 | 4.4:-61161502 | None:intergenic |
| TAAGATCAGTATCTCTTGGA+AGG | 0.574434 | 4.4:-61163861 | None:intergenic |
| GGTGAAATGATAACTGCTGT+TGG | 0.575304 | 4.4:+61161986 | MS.gene34831:CDS |
| AGAATTAACAGATAAAGAGC+AGG | 0.576236 | 4.4:+61168947 | MS.gene34831:CDS |
| GCCTTGCCTACTGGTAATGG+TGG | 0.579377 | 4.4:-61168900 | None:intergenic |
| ATATTTGATACCAGAGACAA+TGG | 0.580022 | 4.4:+61166974 | MS.gene34831:intron |
| TAAAATTTGAAGATACTGAA+AGG | 0.581028 | 4.4:+61168289 | MS.gene34831:CDS |
| CCCACCAAGTGGAAAAGAGA+AGG | 0.589412 | 4.4:+61168224 | MS.gene34831:CDS |
| AAATTCATCTGCTTTCTCTG+TGG | 0.589837 | 4.4:-61167667 | None:intergenic |
| GCCACCATTACCAGTAGGCA+AGG | 0.591193 | 4.4:+61168899 | MS.gene34831:CDS |
| CAACTGCAGTTTGCCTGTGG+TGG | 0.591370 | 4.4:-61167221 | None:intergenic |
| GATATAGCAATATCGCAAGC+TGG | 0.591658 | 4.4:-61168747 | None:intergenic |
| TTTCACCATGAGACAAAAGC+TGG | 0.591764 | 4.4:-61161970 | None:intergenic |
| TCTGGTAAGCGAGATTTGGA+AGG | 0.595760 | 4.4:-61161351 | None:intergenic |
| GTTTCGGAGAGGACAAATCC+AGG | 0.598853 | 4.4:-61161564 | None:intergenic |
| TCCAAATCTCGCTTACCAGA+AGG | 0.599238 | 4.4:+61161354 | MS.gene34831:CDS |
| CAGGAGTTCATCTCCACCAC+AGG | 0.599540 | 4.4:+61167208 | MS.gene34831:CDS |
| CTCGCCTGGTGACGGAGCAG+CGG | 0.600406 | 4.4:-61161385 | None:intergenic |
| TTGCTATATCAGGTCTAGGA+TGG | 0.601269 | 4.4:+61168760 | MS.gene34831:CDS |
| CAGAAACTGTATGACACTCC+AGG | 0.601341 | 4.4:+61167189 | MS.gene34831:intron |
| ACGAGGCCACCATTACCAGT+AGG | 0.618637 | 4.4:+61168894 | MS.gene34831:CDS |
| TTGCTCCTAAAAGCCGACTG+AGG | 0.619264 | 4.4:+61167268 | MS.gene34831:CDS |
| ACAGCCATAGCAAGAAGCGA+CGG | 0.623005 | 4.4:-61161508 | None:intergenic |
| GGAAAGATAGGCCCCTCAGT+CGG | 0.624772 | 4.4:-61167281 | None:intergenic |
| TCTCGCTTACCAGAAGGCAC+CGG | 0.635447 | 4.4:+61161360 | MS.gene34831:CDS |
| CTTTCCGACAATACAAACAA+AGG | 0.637415 | 4.4:+61167405 | MS.gene34831:CDS |
| GAGAAGGCTGAGAACTGGAG+AGG | 0.643970 | 4.4:+61168240 | MS.gene34831:CDS |
| GAAATGATAACTGCTGTTGG+AGG | 0.645323 | 4.4:+61161989 | MS.gene34831:CDS |
| TTAAAACTGTTCTATGTGGG+CGG | 0.648875 | 4.4:+61161945 | MS.gene34831:CDS |
| ACCAGCAAGATCTCTCACTC+GGG | 0.656086 | 4.4:-61163712 | None:intergenic |
| AGGGAGGATAGATCTTCGGA+AGG | 0.666269 | 4.4:-61167246 | None:intergenic |
| ATTACCAGTAGGCAAGGCTG+GGG | 0.666863 | 4.4:+61168905 | MS.gene34831:CDS |
| ACTGCTGTTGGAGGACATGG+AGG | 0.674606 | 4.4:+61161998 | MS.gene34831:CDS |
| GGACATGGAGGATACTCTGG+CGG | 0.680136 | 4.4:+61162010 | MS.gene34831:CDS |
| GAACAACTGCAGTTTGCCTG+TGG | 0.695137 | 4.4:-61167224 | None:intergenic |
| AATGGCATATAATGATCCAG+TGG | 0.696160 | 4.4:+61166992 | MS.gene34831:CDS |
| GCTCCTAAAAGCCGACTGAG+GGG | 0.696766 | 4.4:+61167270 | MS.gene34831:CDS |
| GTTCTGCTAATCCCACCAAG+TGG | 0.707656 | 4.4:+61168213 | MS.gene34831:CDS |
| CCAATTCATAGGTTTCGGAG+AGG | 0.711908 | 4.4:-61161575 | None:intergenic |
| AAAATGCAATAGACACTACG+GGG | 0.732571 | 4.4:+61168826 | MS.gene34831:CDS |
| AATTCGCAGAGAATGAGACA+AGG | 0.739526 | 4.4:-61161321 | None:intergenic |
| AAATGCAATAGACACTACGG+GGG | 0.756248 | 4.4:+61168827 | MS.gene34831:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 61161243 | 61168995 | 61161243 | ID=MS.gene34831 |
| chr4.4 | mRNA | 61161243 | 61168995 | 61161243 | ID=MS.gene34831.t1;Parent=MS.gene34831 |
| chr4.4 | exon | 61161243 | 61161596 | 61161243 | ID=MS.gene34831.t1.exon1;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61161243 | 61161596 | 61161243 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61161926 | 61162111 | 61161926 | ID=MS.gene34831.t1.exon2;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61161926 | 61162111 | 61161926 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61163678 | 61163764 | 61163678 | ID=MS.gene34831.t1.exon3;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61163678 | 61163764 | 61163678 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61163853 | 61163932 | 61163853 | ID=MS.gene34831.t1.exon4;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61163853 | 61163932 | 61163853 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61164221 | 61164302 | 61164221 | ID=MS.gene34831.t1.exon5;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61164221 | 61164302 | 61164221 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61164890 | 61164910 | 61164890 | ID=MS.gene34831.t1.exon6;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61164890 | 61164910 | 61164890 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61165501 | 61165546 | 61165501 | ID=MS.gene34831.t1.exon7;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61165501 | 61165546 | 61165501 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61166988 | 61167106 | 61166988 | ID=MS.gene34831.t1.exon8;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61166988 | 61167106 | 61166988 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61167192 | 61167305 | 61167192 | ID=MS.gene34831.t1.exon9;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61167192 | 61167305 | 61167192 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61167393 | 61167500 | 61167393 | ID=MS.gene34831.t1.exon10;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61167393 | 61167500 | 61167393 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61167606 | 61167695 | 61167606 | ID=MS.gene34831.t1.exon11;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61167606 | 61167695 | 61167606 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61168201 | 61168310 | 61168201 | ID=MS.gene34831.t1.exon12;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61168201 | 61168310 | 61168201 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| chr4.4 | exon | 61168746 | 61168995 | 61168746 | ID=MS.gene34831.t1.exon13;Parent=MS.gene34831.t1 |
| chr4.4 | CDS | 61168746 | 61168995 | 61168746 | ID=cds.MS.gene34831.t1;Parent=MS.gene34831.t1 |
| Gene Sequence |
| Protein sequence |
