Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39755.t1 | XP_013448647.1 | 95.1 | 61 | 3 | 0 | 1 | 61 | 144 | 204 | 3.00E-28 | 133.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39755.t1 | B7FGJ5 | 95.1 | 61 | 3 | 0 | 1 | 61 | 107 | 167 | 2.2e-28 | 133.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene39755 | 0.8546 | 1.10E-61 | -1.69E-46 |
| MS.gene049222 | MS.gene39755 | 0.828176 | 1.04E-54 | -1.69E-46 |
| MS.gene049232 | MS.gene39755 | 0.81079 | 9.68E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene39755 | 0.820603 | 6.29E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene39755 | 0.832662 | 8.32E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene39755 | 0.812174 | 4.84E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene39755 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene39755 | 0.843868 | 1.08E-58 | -1.69E-46 |
| MS.gene05010 | MS.gene39755 | 0.837519 | 4.95E-57 | -1.69E-46 |
| MS.gene050323 | MS.gene39755 | 0.833115 | 6.42E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene39755 | 0.84387 | 1.07E-58 | -1.69E-46 |
| MS.gene050577 | MS.gene39755 | 0.803187 | 3.93E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene39755 | 0.82428 | 8.79E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene39755 | 0.834253 | 3.34E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene39755 | 0.811367 | 7.26E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene39755 | 0.825667 | 4.14E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene39755 | 0.826303 | 2.92E-54 | -1.69E-46 |
| MS.gene05080 | MS.gene39755 | 0.832317 | 1.01E-55 | -1.69E-46 |
| MS.gene05081 | MS.gene39755 | 0.812913 | 3.34E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene39755 | 0.850465 | 1.67E-60 | -1.69E-46 |
| MS.gene050871 | MS.gene39755 | 0.850402 | 1.74E-60 | -1.69E-46 |
| MS.gene050950 | MS.gene39755 | 0.808887 | 2.48E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene39755 | 0.823263 | 1.52E-53 | -1.69E-46 |
| MS.gene051551 | MS.gene39755 | 0.826479 | 2.65E-54 | -1.69E-46 |
| MS.gene051666 | MS.gene39755 | 0.810564 | 1.08E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene39755 | 0.807366 | 5.23E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene39755 | 0.813229 | 2.84E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene39755 | 0.802797 | 4.73E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene39755 | 0.816256 | 6.07E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene39755 | 0.820452 | 6.81E-53 | -1.69E-46 |
| MS.gene052504 | MS.gene39755 | 0.826014 | 3.42E-54 | -1.69E-46 |
| MS.gene052506 | MS.gene39755 | 0.835371 | 1.74E-56 | -1.69E-46 |
| MS.gene052820 | MS.gene39755 | 0.817581 | 3.06E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene39755 | 0.826315 | 2.90E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene39755 | 0.817193 | 3.74E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene39755 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene39755 | 0.845756 | 3.33E-59 | -1.69E-46 |
| MS.gene053103 | MS.gene39755 | 0.814809 | 1.28E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene39755 | 0.824954 | 6.10E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene39755 | 0.835092 | 2.05E-56 | -1.69E-46 |
| MS.gene053207 | MS.gene39755 | 0.818485 | 1.91E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene39755 | 0.818438 | 1.96E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene39755 | 0.818841 | 1.59E-52 | -1.69E-46 |
| MS.gene053436 | MS.gene39755 | 0.816119 | 6.52E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene39755 | 0.815596 | 8.52E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene39755 | 0.81121 | 7.85E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene39755 | 0.810545 | 1.09E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene39755 | 0.808251 | 3.40E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene39755 | 0.813147 | 2.97E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene39755 | 0.82146 | 3.99E-53 | -1.69E-46 |
| MS.gene054003 | MS.gene39755 | 0.823522 | 1.32E-53 | -1.69E-46 |
| MS.gene054408 | MS.gene39755 | 0.804543 | 2.05E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene39755 | 0.858069 | 1.06E-62 | -1.69E-46 |
| MS.gene054600 | MS.gene39755 | 0.818935 | 1.51E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene39755 | 0.829667 | 4.53E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene39755 | 0.813161 | 2.94E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene39755 | 0.822671 | 2.09E-53 | -1.69E-46 |
| MS.gene055224 | MS.gene39755 | 0.811287 | 7.55E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene39755 | 0.804191 | 2.43E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene39755 | 0.839537 | 1.49E-57 | -1.69E-46 |
| MS.gene055502 | MS.gene39755 | 0.835917 | 1.27E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene39755 | 0.827303 | 1.69E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene39755 | 0.820964 | 5.19E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene39755 | 0.816559 | 5.19E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene39755 | 0.804532 | 2.06E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene39755 | 0.811696 | 6.15E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene39755 | 0.818056 | 2.39E-52 | -1.69E-46 |
| MS.gene055941 | MS.gene39755 | 0.819742 | 9.90E-53 | -1.69E-46 |
| MS.gene056166 | MS.gene39755 | 0.807773 | 4.29E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene39755 | 0.840667 | 7.58E-58 | -1.69E-46 |
| MS.gene056422 | MS.gene39755 | 0.805095 | 1.58E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene39755 | 0.829044 | 6.42E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene39755 | 0.830162 | 3.43E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene39755 | 0.80888 | 2.49E-50 | -1.69E-46 |
| MS.gene056878 | MS.gene39755 | 0.800334 | 1.51E-48 | -1.69E-46 |
| MS.gene057086 | MS.gene39755 | 0.852135 | 5.63E-61 | -1.69E-46 |
| MS.gene057331 | MS.gene39755 | 0.822661 | 2.10E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene39755 | 0.844246 | 8.51E-59 | -1.69E-46 |
| MS.gene057734 | MS.gene39755 | 0.803309 | 3.71E-49 | -1.69E-46 |
| MS.gene057856 | MS.gene39755 | 0.819312 | 1.24E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene39755 | 0.824827 | 6.54E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene39755 | 0.831807 | 1.35E-55 | -1.69E-46 |
| MS.gene058544 | MS.gene39755 | 0.818323 | 2.08E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene39755 | 0.833878 | 4.14E-56 | -1.69E-46 |
| MS.gene058838 | MS.gene39755 | 0.840344 | 9.20E-58 | -1.69E-46 |
| MS.gene059022 | MS.gene39755 | 0.834491 | 2.91E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene39755 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene39755 | 0.813142 | 2.97E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene39755 | 0.80791 | 4.01E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene39755 | 0.817675 | 2.92E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene39755 | 0.802416 | 5.67E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene39755 | 0.824066 | 9.87E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene39755 | 0.817091 | 3.95E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene39755 | 0.824802 | 6.63E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene39755 | 0.817746 | 2.81E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene39755 | 0.834328 | 3.19E-56 | -1.69E-46 |
| MS.gene06097 | MS.gene39755 | 0.804639 | 1.96E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene39755 | 0.820764 | 5.77E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene39755 | 0.826656 | 2.41E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39755.t1 | MTR_7g056413 | 95.082 | 61 | 3 | 0 | 1 | 61 | 144 | 204 | 3.51e-37 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCTTATGGGCATGTTTA+TGG | 0.209138 | 7.1:-45607750 | MS.gene39755:CDS |
| CCTTCGCCAGTTGTTCCTTA+TGG | 0.209497 | 7.1:-45607762 | MS.gene39755:CDS |
| CACAAGTACCCTTCAAATTA+TGG | 0.270336 | 7.1:+45607602 | None:intergenic |
| CTTCGCCAGTTGTTCCTTAT+GGG | 0.271859 | 7.1:-45607761 | MS.gene39755:CDS |
| CCATCCATAACTATCGTATA+CGG | 0.371058 | 7.1:+45607689 | None:intergenic |
| TGGGTCATCACCACGATTGC+TGG | 0.388653 | 7.1:-45607650 | MS.gene39755:CDS |
| ACATGCCCATAAGGAACAAC+TGG | 0.446939 | 7.1:+45607756 | None:intergenic |
| GCACCATAAACATGCCCATA+AGG | 0.479031 | 7.1:+45607747 | None:intergenic |
| CGACATGTACCATAATTTGA+AGG | 0.496633 | 7.1:-45607611 | None:intergenic |
| CTTCTTCGCGCCAGCAATCG+TGG | 0.498790 | 7.1:+45607640 | None:intergenic |
| CGATTGCTGGCGCGAAGAAG+AGG | 0.521477 | 7.1:-45607637 | MS.gene39755:CDS |
| CATGTTTATGGTGCTTGTCA+AGG | 0.539807 | 7.1:-45607738 | MS.gene39755:CDS |
| AGGCCCGTATACGATAGTTA+TGG | 0.549421 | 7.1:-45607693 | MS.gene39755:CDS |
| TGGTGATGACCCACGTAACA+AGG | 0.557049 | 7.1:+45607660 | None:intergenic |
| GTTTATGGTGCTTGTCAAGG+TGG | 0.563579 | 7.1:-45607735 | MS.gene39755:CDS |
| TGGCGAAGGATATGCAAGAA+TGG | 0.564765 | 7.1:+45607776 | None:intergenic |
| ATGGAGTGGGCCTTGTTACG+TGG | 0.565179 | 7.1:-45607670 | MS.gene39755:CDS |
| TACGATAGTTATGGATGGAG+TGG | 0.565922 | 7.1:-45607684 | MS.gene39755:CDS |
| ACGATAGTTATGGATGGAGT+GGG | 0.583338 | 7.1:-45607683 | MS.gene39755:CDS |
| GGCCTCCCATAAAACTGTGG+GGG | 0.585436 | 7.1:+45607711 | None:intergenic |
| CATCCATAACTATCGTATAC+GGG | 0.586554 | 7.1:+45607690 | None:intergenic |
| CGGGCCTCCCATAAAACTGT+GGG | 0.590176 | 7.1:+45607709 | None:intergenic |
| TGGAGTGGGCCTTGTTACGT+GGG | 0.611082 | 7.1:-45607669 | MS.gene39755:CDS |
| GCCTCCCATAAAACTGTGGG+GGG | 0.618515 | 7.1:+45607712 | None:intergenic |
| CCATAAGGAACAACTGGCGA+AGG | 0.625985 | 7.1:+45607762 | None:intergenic |
| CCGTATACGATAGTTATGGA+TGG | 0.632117 | 7.1:-45607689 | MS.gene39755:CDS |
| GGATATGCAAGAATGGACAT+TGG | 0.650120 | 7.1:+45607783 | None:intergenic |
| ACGGGCCTCCCATAAAACTG+TGG | 0.672573 | 7.1:+45607708 | None:intergenic |
| GGGCCTCCCATAAAACTGTG+GGG | 0.723372 | 7.1:+45607710 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATCCATAACTATCGTATAC+GGG | + | chr7.1:45607707-45607726 | None:intergenic | 35.0% | |
| CCATCCATAACTATCGTATA+CGG | + | chr7.1:45607708-45607727 | None:intergenic | 35.0% | |
| ACGATAGTTATGGATGGAGT+GGG | - | chr7.1:45607711-45607730 | MS.gene39755:CDS | 40.0% | |
| CATGTTTATGGTGCTTGTCA+AGG | - | chr7.1:45607656-45607675 | MS.gene39755:CDS | 40.0% | |
| CCGTATACGATAGTTATGGA+TGG | - | chr7.1:45607705-45607724 | MS.gene39755:CDS | 40.0% | |
| GTTCCTTATGGGCATGTTTA+TGG | - | chr7.1:45607644-45607663 | MS.gene39755:CDS | 40.0% | |
| TACGATAGTTATGGATGGAG+TGG | - | chr7.1:45607710-45607729 | MS.gene39755:CDS | 40.0% | |
| ACATGCCCATAAGGAACAAC+TGG | + | chr7.1:45607641-45607660 | None:intergenic | 45.0% | |
| AGGCCCGTATACGATAGTTA+TGG | - | chr7.1:45607701-45607720 | MS.gene39755:CDS | 45.0% | |
| CTTCGCCAGTTGTTCCTTAT+GGG | - | chr7.1:45607633-45607652 | MS.gene39755:CDS | 45.0% | |
| GCACCATAAACATGCCCATA+AGG | + | chr7.1:45607650-45607669 | None:intergenic | 45.0% | |
| GTTTATGGTGCTTGTCAAGG+TGG | - | chr7.1:45607659-45607678 | MS.gene39755:CDS | 45.0% | |
| TGGCGAAGGATATGCAAGAA+TGG | + | chr7.1:45607621-45607640 | None:intergenic | 45.0% | |
| CCATAAGGAACAACTGGCGA+AGG | + | chr7.1:45607635-45607654 | None:intergenic | 50.0% | |
| CCTTCGCCAGTTGTTCCTTA+TGG | - | chr7.1:45607632-45607651 | MS.gene39755:CDS | 50.0% | |
| ! | TGGTGATGACCCACGTAACA+AGG | + | chr7.1:45607737-45607756 | None:intergenic | 50.0% |
| ACGGGCCTCCCATAAAACTG+TGG | + | chr7.1:45607689-45607708 | None:intergenic | 55.0% | |
| CGGGCCTCCCATAAAACTGT+GGG | + | chr7.1:45607688-45607707 | None:intergenic | 55.0% | |
| GCCTCCCATAAAACTGTGGG+GGG | + | chr7.1:45607685-45607704 | None:intergenic | 55.0% | |
| GGCCTCCCATAAAACTGTGG+GGG | + | chr7.1:45607686-45607705 | None:intergenic | 55.0% | |
| GGGCCTCCCATAAAACTGTG+GGG | + | chr7.1:45607687-45607706 | None:intergenic | 55.0% | |
| TGGGTCATCACCACGATTGC+TGG | - | chr7.1:45607744-45607763 | MS.gene39755:CDS | 55.0% | |
| !! | ATGGAGTGGGCCTTGTTACG+TGG | - | chr7.1:45607724-45607743 | MS.gene39755:CDS | 55.0% |
| !! | GTGGCCCCCCACAGTTTTAT+GGG | - | chr7.1:45607678-45607697 | MS.gene39755:CDS | 55.0% |
| !! | TGGAGTGGGCCTTGTTACGT+GGG | - | chr7.1:45607725-45607744 | MS.gene39755:CDS | 55.0% |
| CGATTGCTGGCGCGAAGAAG+AGG | - | chr7.1:45607757-45607776 | MS.gene39755:CDS | 60.0% | |
| CTTCTTCGCGCCAGCAATCG+TGG | + | chr7.1:45607757-45607776 | None:intergenic | 60.0% | |
| ! | GGTGGCCCCCCACAGTTTTA+TGG | - | chr7.1:45607677-45607696 | MS.gene39755:CDS | 60.0% |
| !! | GCCCCCCACAGTTTTATGGG+AGG | - | chr7.1:45607681-45607700 | MS.gene39755:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 45607614 | 45607802 | 45607614 | ID=MS.gene39755 |
| chr7.1 | mRNA | 45607614 | 45607802 | 45607614 | ID=MS.gene39755.t1;Parent=MS.gene39755 |
| chr7.1 | exon | 45607614 | 45607802 | 45607614 | ID=MS.gene39755.t1.exon1;Parent=MS.gene39755.t1 |
| chr7.1 | CDS | 45607614 | 45607802 | 45607614 | ID=cds.MS.gene39755.t1;Parent=MS.gene39755.t1 |
| Gene Sequence |
| Protein sequence |
