Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53771.t1 | RHN51642.1 | 92.3 | 610 | 47 | 0 | 1 | 610 | 1 | 610 | 1.60E-238 | 835.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53771.t1 | A8MQR0 | 48.2 | 564 | 264 | 9 | 12 | 573 | 11 | 548 | 2.2e-71 | 271.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53771.t1 | A0A396HE81 | 92.3 | 610 | 47 | 0 | 1 | 610 | 1 | 610 | 1.1e-238 | 835.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049272 | MS.gene53771 | 0.817051 | 4.03E-52 | -1.69E-46 |
| MS.gene04933 | MS.gene53771 | 0.824218 | 9.09E-54 | -1.69E-46 |
| MS.gene049378 | MS.gene53771 | 0.854921 | 8.92E-62 | -1.69E-46 |
| MS.gene049435 | MS.gene53771 | 0.85686 | 2.42E-62 | -1.69E-46 |
| MS.gene049501 | MS.gene53771 | 0.806535 | 7.85E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene53771 | 0.803363 | 3.61E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene53771 | 0.860008 | 2.79E-63 | -1.69E-46 |
| MS.gene050227 | MS.gene53771 | 0.850863 | 1.29E-60 | -1.69E-46 |
| MS.gene050228 | MS.gene53771 | 0.858096 | 1.04E-62 | -1.69E-46 |
| MS.gene050442 | MS.gene53771 | 0.886457 | 3.36E-72 | -1.69E-46 |
| MS.gene050623 | MS.gene53771 | -0.820043 | 8.45E-53 | -1.69E-46 |
| MS.gene050633 | MS.gene53771 | 0.813879 | 2.05E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene53771 | 0.81238 | 4.36E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene53771 | 0.806789 | 6.94E-50 | -1.69E-46 |
| MS.gene050724 | MS.gene53771 | 0.80463 | 1.97E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene53771 | 0.828479 | 8.79E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene53771 | 0.805058 | 1.60E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene53771 | 0.806177 | 9.34E-50 | -1.69E-46 |
| MS.gene050787 | MS.gene53771 | 0.86659 | 2.55E-65 | -1.69E-46 |
| MS.gene050820 | MS.gene53771 | 0.821156 | 4.69E-53 | -1.69E-46 |
| MS.gene050826 | MS.gene53771 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene53771 | 0.807295 | 5.42E-50 | -1.69E-46 |
| MS.gene051147 | MS.gene53771 | 0.844564 | 6.99E-59 | -1.69E-46 |
| MS.gene051198 | MS.gene53771 | 0.814177 | 1.76E-51 | -1.69E-46 |
| MS.gene051210 | MS.gene53771 | 0.807839 | 4.15E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene53771 | 0.819436 | 1.16E-52 | -1.69E-46 |
| MS.gene051568 | MS.gene53771 | 0.85713 | 2.01E-62 | -1.69E-46 |
| MS.gene051800 | MS.gene53771 | 0.804623 | 1.98E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene53771 | 0.822705 | 2.05E-53 | -1.69E-46 |
| MS.gene052042 | MS.gene53771 | 0.808436 | 3.10E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene53771 | 0.806051 | 9.93E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene53771 | 0.803803 | 2.93E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene53771 | 0.84803 | 7.95E-60 | -1.69E-46 |
| MS.gene052384 | MS.gene53771 | 0.805896 | 1.07E-49 | -1.69E-46 |
| MS.gene052389 | MS.gene53771 | 0.837277 | 5.71E-57 | -1.69E-46 |
| MS.gene052464 | MS.gene53771 | 0.820139 | 8.03E-53 | -1.69E-46 |
| MS.gene052586 | MS.gene53771 | 0.831311 | 1.79E-55 | -1.69E-46 |
| MS.gene053147 | MS.gene53771 | 0.845949 | 2.95E-59 | -1.69E-46 |
| MS.gene053166 | MS.gene53771 | 0.827512 | 1.50E-54 | -1.69E-46 |
| MS.gene053221 | MS.gene53771 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene053584 | MS.gene53771 | 0.815646 | 8.31E-52 | -1.69E-46 |
| MS.gene053811 | MS.gene53771 | 0.811189 | 7.93E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene53771 | 0.81856 | 1.84E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene53771 | 0.816273 | 6.02E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene53771 | 0.811895 | 5.57E-51 | -1.69E-46 |
| MS.gene05417 | MS.gene53771 | 0.808512 | 2.99E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene53771 | 0.81652 | 5.30E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene53771 | -0.866597 | 2.54E-65 | -1.69E-46 |
| MS.gene054379 | MS.gene53771 | 0.825362 | 4.89E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene53771 | 0.864811 | 9.29E-65 | -1.69E-46 |
| MS.gene054468 | MS.gene53771 | 0.895849 | 6.48E-76 | -1.69E-46 |
| MS.gene054473 | MS.gene53771 | 0.813544 | 2.43E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene53771 | 0.8141 | 1.83E-51 | -1.69E-46 |
| MS.gene054581 | MS.gene53771 | 0.841859 | 3.68E-58 | -1.69E-46 |
| MS.gene054826 | MS.gene53771 | 0.821745 | 3.43E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene53771 | 0.849414 | 3.28E-60 | -1.69E-46 |
| MS.gene055356 | MS.gene53771 | 0.820043 | 8.45E-53 | -1.69E-46 |
| MS.gene055357 | MS.gene53771 | 0.805581 | 1.25E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene53771 | 0.832604 | 8.60E-56 | -1.69E-46 |
| MS.gene055701 | MS.gene53771 | 0.837203 | 5.97E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene53771 | 0.811013 | 8.66E-51 | -1.69E-46 |
| MS.gene05595 | MS.gene53771 | 0.837575 | 4.79E-57 | -1.69E-46 |
| MS.gene056173 | MS.gene53771 | 0.806013 | 1.01E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene53771 | 0.829945 | 3.87E-55 | -1.69E-46 |
| MS.gene056873 | MS.gene53771 | 0.815861 | 7.44E-52 | -1.69E-46 |
| MS.gene056875 | MS.gene53771 | 0.806624 | 7.52E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene53771 | 0.812043 | 5.17E-51 | -1.69E-46 |
| MS.gene057194 | MS.gene53771 | 0.83842 | 2.91E-57 | -1.69E-46 |
| MS.gene057469 | MS.gene53771 | 0.846924 | 1.60E-59 | -1.69E-46 |
| MS.gene05761 | MS.gene53771 | 0.863895 | 1.80E-64 | -1.69E-46 |
| MS.gene057750 | MS.gene53771 | 0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene53771 | 0.812682 | 3.75E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene53771 | 0.811689 | 6.17E-51 | -1.69E-46 |
| MS.gene058710 | MS.gene53771 | 0.812885 | 3.39E-51 | -1.69E-46 |
| MS.gene05872 | MS.gene53771 | 0.823996 | 1.03E-53 | -1.69E-46 |
| MS.gene058736 | MS.gene53771 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene058935 | MS.gene53771 | 0.835786 | 1.37E-56 | -1.69E-46 |
| MS.gene059128 | MS.gene53771 | 0.823477 | 1.36E-53 | -1.69E-46 |
| MS.gene059272 | MS.gene53771 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene53771 | 0.814612 | 1.41E-51 | -1.69E-46 |
| MS.gene059664 | MS.gene53771 | 0.827061 | 1.93E-54 | -1.69E-46 |
| MS.gene060011 | MS.gene53771 | 0.83681 | 7.52E-57 | -1.69E-46 |
| MS.gene060137 | MS.gene53771 | -0.841155 | 5.64E-58 | -1.69E-46 |
| MS.gene060167 | MS.gene53771 | 0.814846 | 1.25E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene53771 | 0.809727 | 1.64E-50 | -1.69E-46 |
| MS.gene060373 | MS.gene53771 | 0.806429 | 8.26E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene53771 | 0.851521 | 8.41E-61 | -1.69E-46 |
| MS.gene060553 | MS.gene53771 | 0.803858 | 2.85E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene53771 | 0.816743 | 4.72E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene53771 | 0.816916 | 4.32E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene53771 | 0.842631 | 2.30E-58 | -1.69E-46 |
| MS.gene060803 | MS.gene53771 | 0.819821 | 9.50E-53 | -1.69E-46 |
| MS.gene060998 | MS.gene53771 | 0.827172 | 1.81E-54 | -1.69E-46 |
| MS.gene061040 | MS.gene53771 | -0.830531 | 2.79E-55 | -1.69E-46 |
| MS.gene061114 | MS.gene53771 | -0.827142 | 1.84E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53771 | MS.gene025832 | PPI |
| MS.gene065077 | MS.gene53771 | PPI |
| MS.gene53771 | MS.gene22770 | PPI |
| MS.gene024366 | MS.gene53771 | PPI |
| MS.gene53771 | MS.gene45459 | PPI |
| MS.gene29262 | MS.gene53771 | PPI |
| MS.gene026206 | MS.gene53771 | PPI |
| MS.gene53771 | MS.gene88573 | PPI |
| MS.gene53771 | MS.gene050727 | PPI |
| MS.gene53771 | MS.gene003842 | PPI |
| MS.gene53771 | MS.gene91080 | PPI |
| MS.gene53771 | MS.gene029347 | PPI |
| MS.gene53771 | MS.gene76253 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53771.t1 | MTR_3g086660 | 40.336 | 595 | 319 | 11 | 1 | 565 | 1 | 589 | 1.65e-108 | 338 |
| MS.gene53771.t1 | MTR_7g017690 | 37.589 | 564 | 318 | 9 | 16 | 570 | 10 | 548 | 4.58e-92 | 296 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53771.t1 | AT1G68910 | 47.445 | 548 | 263 | 8 | 28 | 573 | 24 | 548 | 1.64e-142 | 426 |
| MS.gene53771.t1 | AT1G68910 | 47.445 | 548 | 263 | 8 | 28 | 573 | 24 | 548 | 1.64e-142 | 426 |
| MS.gene53771.t1 | AT1G68910 | 47.445 | 548 | 263 | 8 | 28 | 573 | 24 | 548 | 6.46e-142 | 426 |
| MS.gene53771.t1 | AT5G11390 | 40.128 | 623 | 321 | 11 | 15 | 593 | 25 | 639 | 3.82e-107 | 338 |
Find 85 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATTACAACAACAGTTAT+TGG | 0.208222 | 6.1:+42421312 | MS.gene53771:CDS |
| TTCTACCATTCTCTGATCTT+TGG | 0.221608 | 6.1:-42421561 | None:intergenic |
| GAAGAGCTGAACTTTCTTAA+AGG | 0.224825 | 6.1:+42422202 | MS.gene53771:CDS |
| ACACTTCAATAAAACCTTTC+AGG | 0.255369 | 6.1:+42423389 | MS.gene53771:CDS |
| CAATGCTACCGAGGTGTTAA+TGG | 0.262222 | 6.1:+42421766 | MS.gene53771:CDS |
| AACAAAGTTTATCATGGATT+TGG | 0.269879 | 6.1:+42422576 | MS.gene53771:CDS |
| ATATGTTATACACAGCAATT+TGG | 0.281088 | 6.1:+42422338 | MS.gene53771:CDS |
| AATTGAAGAGCTAAAATCTA+AGG | 0.296015 | 6.1:+42422375 | MS.gene53771:CDS |
| TGTTCTGTATATTTATCTAT+TGG | 0.302396 | 6.1:+42421039 | MS.gene53771:CDS |
| ATACACAGCAATTTGGGATA+TGG | 0.307575 | 6.1:+42422345 | MS.gene53771:CDS |
| GGGGATTTCGAAAAGTATAA+TGG | 0.309555 | 6.1:+42421787 | MS.gene53771:CDS |
| AAGTTTGGTAGGGAAGTTAA+AGG | 0.312595 | 6.1:+42423251 | MS.gene53771:CDS |
| AAGAAGCAGCAGAAGTTGTT+TGG | 0.325223 | 6.1:+42421723 | MS.gene53771:CDS |
| ATCCATGATAAACTTTGTTC+TGG | 0.336896 | 6.1:-42422572 | None:intergenic |
| TACTGAGGTGTTGGATTTGA+GGG | 0.336994 | 6.1:+42421970 | MS.gene53771:CDS |
| ATTCATATACACAGGGGAAA+TGG | 0.346219 | 6.1:+42421484 | MS.gene53771:intron |
| CCGATGATCTATTTCTGTTA+AGG | 0.349046 | 6.1:-42420981 | None:intergenic |
| AAAACAGGAAAATAAAAGTT+TGG | 0.349494 | 6.1:+42423236 | MS.gene53771:CDS |
| AGAAGCAGCAGAAGTTGTTT+GGG | 0.356426 | 6.1:+42421724 | MS.gene53771:CDS |
| TTCATATACACAGGGGAAAT+GGG | 0.379165 | 6.1:+42421485 | MS.gene53771:intron |
| AATGCTACCGAGGTGTTAAT+GGG | 0.379167 | 6.1:+42421767 | MS.gene53771:CDS |
| CCAGCTCTTTATGAAGTTCA+AGG | 0.382440 | 6.1:-42422462 | None:intergenic |
| AAGAGCTGAACTTTCTTAAA+GGG | 0.392409 | 6.1:+42422203 | MS.gene53771:CDS |
| TATGTTATACACAGCAATTT+GGG | 0.393418 | 6.1:+42422339 | MS.gene53771:CDS |
| GCTTTGCATGAAAACATCAT+TGG | 0.410569 | 6.1:+42422504 | MS.gene53771:CDS |
| ATGCCTTCGCATTCTGTAGT+TGG | 0.418735 | 6.1:-42422288 | None:intergenic |
| GGGATTTCGAAAAGTATAAT+GGG | 0.426529 | 6.1:+42421788 | MS.gene53771:CDS |
| TACCAGAACAAAGTTTATCA+TGG | 0.428221 | 6.1:+42422570 | MS.gene53771:CDS |
| TAATTGCTTCTTAGACTCTT+CGG | 0.428486 | 6.1:-42421296 | None:intergenic |
| ATGCAAGTTCACCAGCTTCT+CGG | 0.428648 | 6.1:-42421017 | None:intergenic |
| AAGTTAAAGGAGACTAAAAT+TGG | 0.430349 | 6.1:+42423264 | MS.gene53771:CDS |
| TGAGGTTGAACTCAGTTACT+TGG | 0.431369 | 6.1:-42421817 | None:intergenic |
| AAGGGAGCGCATTAATAAAC+AGG | 0.433397 | 6.1:+42422618 | MS.gene53771:CDS |
| ATGCTGCTTTAGAGAAGAAC+GGG | 0.446606 | 6.1:+42421912 | MS.gene53771:CDS |
| CAGGAAAATAAAAGTTTGGT+AGG | 0.448633 | 6.1:+42423240 | MS.gene53771:CDS |
| ATACTGAGGTGTTGGATTTG+AGG | 0.449553 | 6.1:+42421969 | MS.gene53771:CDS |
| CACCAGCTTCTCGGAAGAAT+AGG | 0.454696 | 6.1:-42421008 | None:intergenic |
| AACAGAGCTTGACAATTTCA+TGG | 0.456939 | 6.1:+42421174 | MS.gene53771:CDS |
| ATGATGTTTGATCTGTTGTC+TGG | 0.458775 | 6.1:+42421133 | MS.gene53771:CDS |
| GCAAGGATATATCAAGATCT+TGG | 0.461436 | 6.1:-42420955 | None:intergenic |
| GCAGCTAGATGCTTTAAAAC+AGG | 0.463620 | 6.1:+42423221 | MS.gene53771:intron |
| ACAAAATCATTTGATGAAGA+AGG | 0.480014 | 6.1:+42423360 | MS.gene53771:CDS |
| TATTTCTGTTAAGGCTTGCA+AGG | 0.481548 | 6.1:-42420972 | None:intergenic |
| ATCATTTGATGAAGAAGGAA+TGG | 0.487382 | 6.1:+42423365 | MS.gene53771:CDS |
| AGCCTATTCTTCCGAGAAGC+TGG | 0.487690 | 6.1:+42421006 | MS.gene53771:CDS |
| TCAACTTACAATTCTCATGT+TGG | 0.490472 | 6.1:-42421381 | None:intergenic |
| TCAGGAAAATACTGAGGTGT+TGG | 0.490567 | 6.1:+42421961 | MS.gene53771:CDS |
| ATCATTGCTTGAGTCAATGT+TGG | 0.490619 | 6.1:-42423331 | None:intergenic |
| TTCTATAAGATGTTCATGAC+AGG | 0.491221 | 6.1:-42422068 | None:intergenic |
| AAAATTGGTGCATGCAACAA+TGG | 0.496724 | 6.1:+42423279 | MS.gene53771:CDS |
| AAATTGGTGCATGCAACAAT+GGG | 0.516712 | 6.1:+42423280 | MS.gene53771:CDS |
| CTCGCTGCATATAACCGTTG+AGG | 0.524764 | 6.1:-42421835 | None:intergenic |
| TGCTTGAGAAGTCGCTCGCA+AGG | 0.526338 | 6.1:+42421606 | MS.gene53771:CDS |
| GGTGTTGGATTTGAGGGAAA+AGG | 0.526687 | 6.1:+42421976 | MS.gene53771:CDS |
| GCGAAGGCATCTTCTGAAGC+AGG | 0.527456 | 6.1:+42422301 | MS.gene53771:CDS |
| CCTTGAACTTCATAAAGAGC+TGG | 0.546083 | 6.1:+42422462 | MS.gene53771:CDS |
| CTTAGAAAACTCATTTGAGA+AGG | 0.549658 | 6.1:+42421108 | MS.gene53771:CDS |
| AGCTGAGAGTGCTGAAGCAA+AGG | 0.555426 | 6.1:+42422144 | MS.gene53771:CDS |
| AGATCTTGAAAAGAAGTTAG+CGG | 0.557654 | 6.1:+42421634 | MS.gene53771:CDS |
| TAATGCAACTAGCTACTGAA+AGG | 0.557736 | 6.1:+42422599 | MS.gene53771:CDS |
| TGTTAATGTTGACGATACTC+AGG | 0.562953 | 6.1:+42421943 | MS.gene53771:CDS |
| GAAAACCAAAGATCAGAGAA+TGG | 0.567239 | 6.1:+42421556 | MS.gene53771:CDS |
| AGGAAAATAAAAGTTTGGTA+GGG | 0.572153 | 6.1:+42423241 | MS.gene53771:CDS |
| CGAAATCCCCATTAACACCT+CGG | 0.586371 | 6.1:-42421774 | None:intergenic |
| ATACACAGGGGAAATGGGGA+AGG | 0.589178 | 6.1:+42421490 | MS.gene53771:intron |
| ACCTCAGTCAAATGTCTGCA+CGG | 0.591663 | 6.1:-42421241 | None:intergenic |
| ACTCCGCTACACTGAACAAG+TGG | 0.600856 | 6.1:+42421688 | MS.gene53771:CDS |
| GAAGCAGCAGAAGTTGTTTG+GGG | 0.601416 | 6.1:+42421725 | MS.gene53771:CDS |
| ACAGTTAACTGAAACGAACA+TGG | 0.602578 | 6.1:+42422171 | MS.gene53771:CDS |
| TCTAAGGTTTCAAAAGCTGA+AGG | 0.603452 | 6.1:+42422391 | MS.gene53771:CDS |
| GCAGCTTCAGAAAAGTCTTG+TGG | 0.605005 | 6.1:+42421354 | MS.gene53771:CDS |
| AATGCAACTAGCTACTGAAA+GGG | 0.605712 | 6.1:+42422600 | MS.gene53771:CDS |
| TCATATACACAGGGGAAATG+GGG | 0.609571 | 6.1:+42421486 | MS.gene53771:intron |
| GATGCTGCTTTAGAGAAGAA+CGG | 0.611153 | 6.1:+42421911 | MS.gene53771:CDS |
| ATGCCACTTGTTCAGTGTAG+CGG | 0.624346 | 6.1:-42421691 | None:intergenic |
| GTAACTGAGTTCAACCTCAA+CGG | 0.629441 | 6.1:+42421821 | MS.gene53771:CDS |
| CCTTAACAGAAATAGATCAT+CGG | 0.629560 | 6.1:+42420981 | MS.gene53771:CDS |
| GTTCGCTTGAGAAGCAACTG+AGG | 0.632474 | 6.1:+42422254 | MS.gene53771:CDS |
| TTCGCTTGAGAAGCAACTGA+GGG | 0.639113 | 6.1:+42422255 | MS.gene53771:CDS |
| GCTTGAGAAGTCGCTCGCAA+GGG | 0.645249 | 6.1:+42421607 | MS.gene53771:CDS |
| TATCCAACTACAGAATGCGA+AGG | 0.657458 | 6.1:+42422285 | MS.gene53771:CDS |
| ATGCTACCGAGGTGTTAATG+GGG | 0.669958 | 6.1:+42421768 | MS.gene53771:CDS |
| TGAAGCAGACAATGCTACCG+AGG | 0.684617 | 6.1:+42421757 | MS.gene53771:CDS |
| GCCGTGCAGACATTTGACTG+AGG | 0.706871 | 6.1:+42421240 | MS.gene53771:CDS |
| CGATACTCAGGAAAATACTG+AGG | 0.718119 | 6.1:+42421955 | MS.gene53771:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTATGTTTTTATCTTTTA+TGG | + | chr6.1:42422668-42422687 | MS.gene53771:intron | 15.0% |
| !! | AAAACAGGAAAATAAAAGTT+TGG | + | chr6.1:42423236-42423255 | MS.gene53771:CDS | 20.0% |
| !! | AATTGAACTGAAAATATTCA+AGG | + | chr6.1:42422762-42422781 | MS.gene53771:intron | 20.0% |
| !! | TGTTCTGTATATTTATCTAT+TGG | + | chr6.1:42421039-42421058 | MS.gene53771:CDS | 20.0% |
| !!! | AATTTTAATCGAACAACTTA+AGG | + | chr6.1:42421883-42421902 | MS.gene53771:CDS | 20.0% |
| !!! | ATTTTCACAGTTTCTTAATA+TGG | - | chr6.1:42422907-42422926 | None:intergenic | 20.0% |
| !!! | TGAATATTTTCAGTTCAATT+TGG | - | chr6.1:42422762-42422781 | None:intergenic | 20.0% |
| ! | AACAAAGTTTATCATGGATT+TGG | + | chr6.1:42422576-42422595 | MS.gene53771:CDS | 25.0% |
| ! | AAGTTAAAGGAGACTAAAAT+TGG | + | chr6.1:42423264-42423283 | MS.gene53771:CDS | 25.0% |
| ! | AATTGAAGAGCTAAAATCTA+AGG | + | chr6.1:42422375-42422394 | MS.gene53771:CDS | 25.0% |
| ! | ACAAAATCATTTGATGAAGA+AGG | + | chr6.1:42423360-42423379 | MS.gene53771:CDS | 25.0% |
| ! | AGGAAAATAAAAGTTTGGTA+GGG | + | chr6.1:42423241-42423260 | MS.gene53771:CDS | 25.0% |
| ! | ATATGTTATACACAGCAATT+TGG | + | chr6.1:42422338-42422357 | MS.gene53771:CDS | 25.0% |
| ! | GTTACATACATCAATGTATT+AGG | - | chr6.1:42422859-42422878 | None:intergenic | 25.0% |
| ! | TATGTTATACACAGCAATTT+GGG | + | chr6.1:42422339-42422358 | MS.gene53771:CDS | 25.0% |
| ! | TTGCATGAAATGATCATTAA+AGG | - | chr6.1:42422708-42422727 | None:intergenic | 25.0% |
| ! | TTTAGTCATTCATATACACA+GGG | + | chr6.1:42421477-42421496 | MS.gene53771:intron | 25.0% |
| !! | ACGATACTGTTATTGATTAA+TGG | - | chr6.1:42423152-42423171 | None:intergenic | 25.0% |
| !! | TTTTAGTCATTCATATACAC+AGG | + | chr6.1:42421476-42421495 | MS.gene53771:intron | 25.0% |
| !!! | AATGCTTTCTTTTAGAGATT+CGG | - | chr6.1:42422107-42422126 | None:intergenic | 25.0% |
| AACATAGAACTTATTTGACG+AGG | - | chr6.1:42422657-42422676 | None:intergenic | 30.0% | |
| AAGAGCTGAACTTTCTTAAA+GGG | + | chr6.1:42422203-42422222 | MS.gene53771:CDS | 30.0% | |
| AGATCTTGAAAAGAAGTTAG+CGG | + | chr6.1:42421634-42421653 | MS.gene53771:CDS | 30.0% | |
| ATCATTTGATGAAGAAGGAA+TGG | + | chr6.1:42423365-42423384 | MS.gene53771:CDS | 30.0% | |
| ATCCATGATAAACTTTGTTC+TGG | - | chr6.1:42422575-42422594 | None:intergenic | 30.0% | |
| CAGGAAAATAAAAGTTTGGT+AGG | + | chr6.1:42423240-42423259 | MS.gene53771:CDS | 30.0% | |
| CCTTAACAGAAATAGATCAT+CGG | + | chr6.1:42420981-42421000 | MS.gene53771:CDS | 30.0% | |
| CTTAGAAAACTCATTTGAGA+AGG | + | chr6.1:42421108-42421127 | MS.gene53771:CDS | 30.0% | |
| GAAAGCTAAACATGAAGATA+AGG | - | chr6.1:42423070-42423089 | None:intergenic | 30.0% | |
| GCAATTACAACAACAGTTAT+TGG | + | chr6.1:42421312-42421331 | MS.gene53771:CDS | 30.0% | |
| TAATTGCTTCTTAGACTCTT+CGG | - | chr6.1:42421299-42421318 | None:intergenic | 30.0% | |
| TACCAGAACAAAGTTTATCA+TGG | + | chr6.1:42422570-42422589 | MS.gene53771:CDS | 30.0% | |
| TCAACTTACAATTCTCATGT+TGG | - | chr6.1:42421384-42421403 | None:intergenic | 30.0% | |
| TGTAGTACATTCTAGATTGA+TGG | - | chr6.1:42422995-42423014 | None:intergenic | 30.0% | |
| TTAGTCATTCATATACACAG+GGG | + | chr6.1:42421478-42421497 | MS.gene53771:intron | 30.0% | |
| TTCTATAAGATGTTCATGAC+AGG | - | chr6.1:42422071-42422090 | None:intergenic | 30.0% | |
| ! | AGAAGAACGAAAGAATTTTG+AGG | + | chr6.1:42422012-42422031 | MS.gene53771:CDS | 30.0% |
| ! | GACTGAGGTTTTCTTTATAA+TGG | + | chr6.1:42421255-42421274 | MS.gene53771:CDS | 30.0% |
| ! | GGGATTTCGAAAAGTATAAT+GGG | + | chr6.1:42421788-42421807 | MS.gene53771:CDS | 30.0% |
| ! | TCTTTTATGGAATCTCAGTT+TGG | + | chr6.1:42422681-42422700 | MS.gene53771:intron | 30.0% |
| ! | TGAACAAGTGGCATTTTATA+TGG | + | chr6.1:42421700-42421719 | MS.gene53771:CDS | 30.0% |
| ! | TTTTCAGTTCAATTTGGACT+TGG | - | chr6.1:42422756-42422775 | None:intergenic | 30.0% |
| !! | ACTTGATAATTTTGTACTGC+TGG | - | chr6.1:42422536-42422555 | None:intergenic | 30.0% |
| AAAATTGGTGCATGCAACAA+TGG | + | chr6.1:42423279-42423298 | MS.gene53771:CDS | 35.0% | |
| AAATTGGTGCATGCAACAAT+GGG | + | chr6.1:42423280-42423299 | MS.gene53771:CDS | 35.0% | |
| AACAGAGCTTGACAATTTCA+TGG | + | chr6.1:42421174-42421193 | MS.gene53771:CDS | 35.0% | |
| AAGTTTGGTAGGGAAGTTAA+AGG | + | chr6.1:42423251-42423270 | MS.gene53771:CDS | 35.0% | |
| AATGCAACTAGCTACTGAAA+GGG | + | chr6.1:42422600-42422619 | MS.gene53771:CDS | 35.0% | |
| ACAGTTAACTGAAACGAACA+TGG | + | chr6.1:42422171-42422190 | MS.gene53771:CDS | 35.0% | |
| AGAAAAACTCTACTCACTCA+AGG | + | chr6.1:42420907-42420926 | MS.gene53771:CDS | 35.0% | |
| ATCATTGCTTGAGTCAATGT+TGG | - | chr6.1:42423334-42423353 | None:intergenic | 35.0% | |
| ATGATGTTTGATCTGTTGTC+TGG | + | chr6.1:42421133-42421152 | MS.gene53771:CDS | 35.0% | |
| ATTCATATACACAGGGGAAA+TGG | + | chr6.1:42421484-42421503 | MS.gene53771:intron | 35.0% | |
| GAAAAACTCTACTCACTCAA+GGG | + | chr6.1:42420908-42420927 | MS.gene53771:CDS | 35.0% | |
| GAAAACCAAAGATCAGAGAA+TGG | + | chr6.1:42421556-42421575 | MS.gene53771:CDS | 35.0% | |
| GAAGAGCTGAACTTTCTTAA+AGG | + | chr6.1:42422202-42422221 | MS.gene53771:CDS | 35.0% | |
| GCAAGGATATATCAAGATCT+TGG | - | chr6.1:42420958-42420977 | None:intergenic | 35.0% | |
| GCTTTGCATGAAAACATCAT+TGG | + | chr6.1:42422504-42422523 | MS.gene53771:CDS | 35.0% | |
| TAATGCAACTAGCTACTGAA+AGG | + | chr6.1:42422599-42422618 | MS.gene53771:CDS | 35.0% | |
| TGTTAATGTTGACGATACTC+AGG | + | chr6.1:42421943-42421962 | MS.gene53771:CDS | 35.0% | |
| TTCATATACACAGGGGAAAT+GGG | + | chr6.1:42421485-42421504 | MS.gene53771:intron | 35.0% | |
| TTCTACCATTCTCTGATCTT+TGG | - | chr6.1:42421564-42421583 | None:intergenic | 35.0% | |
| ! | AAATGAGTTTTCTAAGTCGC+AGG | - | chr6.1:42421104-42421123 | None:intergenic | 35.0% |
| ! | ACTCATTAGAGCATCTTCAA+TGG | + | chr6.1:42422822-42422841 | MS.gene53771:intron | 35.0% |
| ! | ATACACAGCAATTTGGGATA+TGG | + | chr6.1:42422345-42422364 | MS.gene53771:CDS | 35.0% |
| ! | CTCATTAGAGCATCTTCAAT+GGG | + | chr6.1:42422823-42422842 | MS.gene53771:intron | 35.0% |
| ! | GGACTTGGTATACAAAGTTA+AGG | - | chr6.1:42422741-42422760 | None:intergenic | 35.0% |
| ! | GGGGATTTCGAAAAGTATAA+TGG | + | chr6.1:42421787-42421806 | MS.gene53771:CDS | 35.0% |
| ! | GTCTGGCATTTTAGATTTTG+AGG | + | chr6.1:42421150-42421169 | MS.gene53771:CDS | 35.0% |
| ! | TATTTCTGTTAAGGCTTGCA+AGG | - | chr6.1:42420975-42420994 | None:intergenic | 35.0% |
| ! | TCTAAGGTTTCAAAAGCTGA+AGG | + | chr6.1:42422391-42422410 | MS.gene53771:CDS | 35.0% |
| !! | CCGATGATCTATTTCTGTTA+AGG | - | chr6.1:42420984-42421003 | None:intergenic | 35.0% |
| !!! | AGCTTTCCTTGTTTTACTCT+TGG | + | chr6.1:42423083-42423102 | MS.gene53771:intron | 35.0% |
| AAGAAGCAGCAGAAGTTGTT+TGG | + | chr6.1:42421723-42421742 | MS.gene53771:CDS | 40.0% | |
| AGAAGCAGCAGAAGTTGTTT+GGG | + | chr6.1:42421724-42421743 | MS.gene53771:CDS | 40.0% | |
| AGTACTGCCGAGAAAAAAGT+CGG | + | chr6.1:42422232-42422251 | MS.gene53771:CDS | 40.0% | |
| CCAACTCCAAGAGTAAAACA+AGG | - | chr6.1:42423092-42423111 | None:intergenic | 40.0% | |
| CCAGCTCTTTATGAAGTTCA+AGG | - | chr6.1:42422465-42422484 | None:intergenic | 40.0% | |
| CCTTGAACTTCATAAAGAGC+TGG | + | chr6.1:42422462-42422481 | MS.gene53771:CDS | 40.0% | |
| CGATACTCAGGAAAATACTG+AGG | + | chr6.1:42421955-42421974 | MS.gene53771:CDS | 40.0% | |
| GTAACTGAGTTCAACCTCAA+CGG | + | chr6.1:42421821-42421840 | MS.gene53771:CDS | 40.0% | |
| TAAGGAAAGCTTGCAGTACA+AGG | - | chr6.1:42423052-42423071 | None:intergenic | 40.0% | |
| TATCCAACTACAGAATGCGA+AGG | + | chr6.1:42422285-42422304 | MS.gene53771:CDS | 40.0% | |
| TCAGGAAAATACTGAGGTGT+TGG | + | chr6.1:42421961-42421980 | MS.gene53771:CDS | 40.0% | |
| TCATATACACAGGGGAAATG+GGG | + | chr6.1:42421486-42421505 | MS.gene53771:intron | 40.0% | |
| TGAGGTTGAACTCAGTTACT+TGG | - | chr6.1:42421820-42421839 | None:intergenic | 40.0% | |
| ! | AAGGGAGCGCATTAATAAAC+AGG | + | chr6.1:42422618-42422637 | MS.gene53771:CDS | 40.0% |
| ! | AATGCTACCGAGGTGTTAAT+GGG | + | chr6.1:42421767-42421786 | MS.gene53771:CDS | 40.0% |
| !! | ATACTGAGGTGTTGGATTTG+AGG | + | chr6.1:42421969-42421988 | MS.gene53771:CDS | 40.0% |
| !! | ATGCTGCTTTAGAGAAGAAC+GGG | + | chr6.1:42421912-42421931 | MS.gene53771:CDS | 40.0% |
| !! | GATGCTGCTTTAGAGAAGAA+CGG | + | chr6.1:42421911-42421930 | MS.gene53771:CDS | 40.0% |
| !! | GCAGCTAGATGCTTTAAAAC+AGG | + | chr6.1:42423221-42423240 | MS.gene53771:intron | 40.0% |
| !! | TACTGAGGTGTTGGATTTGA+GGG | + | chr6.1:42421970-42421989 | MS.gene53771:CDS | 40.0% |
| !!! | AAGCGAACCGACTTTTTTCT+CGG | - | chr6.1:42422242-42422261 | None:intergenic | 40.0% |
| !!! | CCTTGTTTTACTCTTGGAGT+TGG | + | chr6.1:42423089-42423108 | MS.gene53771:intron | 40.0% |
| !!! | GTTTTAAAGCATCTAGCTGC+AGG | - | chr6.1:42423221-42423240 | None:intergenic | 40.0% |
| ACCTCAGTCAAATGTCTGCA+CGG | - | chr6.1:42421244-42421263 | None:intergenic | 45.0% | |
| AGGAAGAACGTAGTGGTAAC+TGG | - | chr6.1:42423201-42423220 | None:intergenic | 45.0% | |
| ATGCAAGTTCACCAGCTTCT+CGG | - | chr6.1:42421020-42421039 | None:intergenic | 45.0% | |
| ATGCCTTCGCATTCTGTAGT+TGG | - | chr6.1:42422291-42422310 | None:intergenic | 45.0% | |
| CGAAATCCCCATTAACACCT+CGG | - | chr6.1:42421777-42421796 | None:intergenic | 45.0% | |
| GAAGCAGCAGAAGTTGTTTG+GGG | + | chr6.1:42421725-42421744 | MS.gene53771:CDS | 45.0% | |
| GCAGCTTCAGAAAAGTCTTG+TGG | + | chr6.1:42421354-42421373 | MS.gene53771:CDS | 45.0% | |
| TTCGCTTGAGAAGCAACTGA+GGG | + | chr6.1:42422255-42422274 | MS.gene53771:CDS | 45.0% | |
| ! | ATGCCACTTGTTCAGTGTAG+CGG | - | chr6.1:42421694-42421713 | None:intergenic | 45.0% |
| ! | ATGCTACCGAGGTGTTAATG+GGG | + | chr6.1:42421768-42421787 | MS.gene53771:CDS | 45.0% |
| ! | CAATGCTACCGAGGTGTTAA+TGG | + | chr6.1:42421766-42421785 | MS.gene53771:CDS | 45.0% |
| ! | GGTGTTGGATTTGAGGGAAA+AGG | + | chr6.1:42421976-42421995 | MS.gene53771:CDS | 45.0% |
| ACTCCGCTACACTGAACAAG+TGG | + | chr6.1:42421688-42421707 | MS.gene53771:CDS | 50.0% | |
| AGCCTATTCTTCCGAGAAGC+TGG | + | chr6.1:42421006-42421025 | MS.gene53771:CDS | 50.0% | |
| ATACACAGGGGAAATGGGGA+AGG | + | chr6.1:42421490-42421509 | MS.gene53771:intron | 50.0% | |
| CACCAGCTTCTCGGAAGAAT+AGG | - | chr6.1:42421011-42421030 | None:intergenic | 50.0% | |
| CTCGCTGCATATAACCGTTG+AGG | - | chr6.1:42421838-42421857 | None:intergenic | 50.0% | |
| GTTCGCTTGAGAAGCAACTG+AGG | + | chr6.1:42422254-42422273 | MS.gene53771:CDS | 50.0% | |
| TAGCTGCAGGAAGAACGTAG+TGG | - | chr6.1:42423208-42423227 | None:intergenic | 50.0% | |
| TGAAGCAGACAATGCTACCG+AGG | + | chr6.1:42421757-42421776 | MS.gene53771:CDS | 50.0% | |
| !! | AGCTGAGAGTGCTGAAGCAA+AGG | + | chr6.1:42422144-42422163 | MS.gene53771:CDS | 50.0% |
| GCCGTGCAGACATTTGACTG+AGG | + | chr6.1:42421240-42421259 | MS.gene53771:CDS | 55.0% | |
| GCTTGAGAAGTCGCTCGCAA+GGG | + | chr6.1:42421607-42421626 | MS.gene53771:CDS | 55.0% | |
| TGCTTGAGAAGTCGCTCGCA+AGG | + | chr6.1:42421606-42421625 | MS.gene53771:CDS | 55.0% | |
| !! | GCGAAGGCATCTTCTGAAGC+AGG | + | chr6.1:42422301-42422320 | MS.gene53771:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 42420893 | 42423410 | 42420893 | ID=MS.gene53771 |
| chr6.1 | mRNA | 42420893 | 42423410 | 42420893 | ID=MS.gene53771.t1;Parent=MS.gene53771 |
| chr6.1 | exon | 42420893 | 42421394 | 42420893 | ID=MS.gene53771.t1.exon1;Parent=MS.gene53771.t1 |
| chr6.1 | CDS | 42420893 | 42421394 | 42420893 | ID=cds.MS.gene53771.t1;Parent=MS.gene53771.t1 |
| chr6.1 | exon | 42421498 | 42422639 | 42421498 | ID=MS.gene53771.t1.exon2;Parent=MS.gene53771.t1 |
| chr6.1 | CDS | 42421498 | 42422639 | 42421498 | ID=cds.MS.gene53771.t1;Parent=MS.gene53771.t1 |
| chr6.1 | exon | 42423225 | 42423410 | 42423225 | ID=MS.gene53771.t1.exon3;Parent=MS.gene53771.t1 |
| chr6.1 | CDS | 42423225 | 42423410 | 42423225 | ID=cds.MS.gene53771.t1;Parent=MS.gene53771.t1 |
| Gene Sequence |
| Protein sequence |
