AlfalfaGEDB | MS.gene56245

M. sativa cultivar XinJiangDaYe / MS.gene56245

KEGG: K09874
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56245.t1	XP_003598246.1	97.6	245	6	0	1	245	1	245	6.00E-118	433.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56245.t1	P49173	66.7	228	76	0	16	243	33	260	4.9e-84	312.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56245.t1	G7IYK0	97.6	245	6	0	1	245	1	245	4.4e-118	433.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene56245	0.867452	1.35E-65	-1.69E-46
MS.gene049173	MS.gene56245	0.821134	4.74E-53	-1.69E-46
MS.gene049217	MS.gene56245	0.829943	3.88E-55	-1.69E-46
MS.gene049222	MS.gene56245	0.817092	3.94E-52	-1.69E-46
MS.gene049285	MS.gene56245	0.867057	1.81E-65	-1.69E-46
MS.gene049320	MS.gene56245	0.850446	1.69E-60	-1.69E-46
MS.gene049609	MS.gene56245	0.800691	1.28E-48	-1.69E-46
MS.gene049633	MS.gene56245	0.812212	4.75E-51	-1.69E-46
MS.gene050163	MS.gene56245	0.846107	2.67E-59	-1.69E-46
MS.gene050165	MS.gene56245	0.842546	2.42E-58	-1.69E-46
MS.gene050333	MS.gene56245	0.815805	7.66E-52	-1.69E-46
MS.gene050444	MS.gene56245	0.85097	1.20E-60	-1.69E-46
MS.gene050504	MS.gene56245	0.841119	5.77E-58	-1.69E-46
MS.gene050505	MS.gene56245	-0.825362	4.89E-54	-1.69E-46
MS.gene050518	MS.gene56245	0.805892	1.07E-49	-1.69E-46
MS.gene050538	MS.gene56245	0.81733	3.49E-52	-1.69E-46
MS.gene050612	MS.gene56245	0.861777	8.08E-64	-1.69E-46
MS.gene050699	MS.gene56245	0.839366	1.65E-57	-1.69E-46
MS.gene050772	MS.gene56245	0.820618	6.24E-53	-1.69E-46
MS.gene05080	MS.gene56245	0.806322	8.70E-50	-1.69E-46
MS.gene05081	MS.gene56245	0.82283	1.92E-53	-1.69E-46
MS.gene050986	MS.gene56245	0.821602	3.70E-53	-1.69E-46
MS.gene05100	MS.gene56245	0.820723	5.90E-53	-1.69E-46
MS.gene051228	MS.gene56245	0.851223	1.02E-60	-1.69E-46
MS.gene051244	MS.gene56245	0.817274	3.59E-52	-1.69E-46
MS.gene051317	MS.gene56245	0.828916	6.89E-55	-1.69E-46
MS.gene051617	MS.gene56245	0.83084	2.34E-55	-1.69E-46
MS.gene051666	MS.gene56245	0.809417	1.91E-50	-1.69E-46
MS.gene051810	MS.gene56245	0.873259	1.69E-67	-1.69E-46
MS.gene051838	MS.gene56245	0.811358	7.29E-51	-1.69E-46
MS.gene051858	MS.gene56245	0.804166	2.46E-49	-1.69E-46
MS.gene051949	MS.gene56245	0.840088	1.07E-57	-1.69E-46
MS.gene052129	MS.gene56245	-0.823833	1.12E-53	-1.69E-46
MS.gene052271	MS.gene56245	0.804179	2.45E-49	-1.69E-46
MS.gene052379	MS.gene56245	0.843767	1.14E-58	-1.69E-46
MS.gene052615	MS.gene56245	0.801355	9.36E-49	-1.69E-46
MS.gene052870	MS.gene56245	0.862375	5.30E-64	-1.69E-46
MS.gene052872	MS.gene56245	0.869816	2.33E-66	-1.69E-46
MS.gene053104	MS.gene56245	0.823347	1.45E-53	-1.69E-46
MS.gene053207	MS.gene56245	0.819203	1.31E-52	-1.69E-46
MS.gene053213	MS.gene56245	0.811502	6.78E-51	-1.69E-46
MS.gene053286	MS.gene56245	0.811884	5.60E-51	-1.69E-46
MS.gene053632	MS.gene56245	0.848179	7.23E-60	-1.69E-46
MS.gene053824	MS.gene56245	0.815416	9.35E-52	-1.69E-46
MS.gene053916	MS.gene56245	0.803917	2.77E-49	-1.69E-46
MS.gene054600	MS.gene56245	0.858545	7.66E-63	-1.69E-46
MS.gene054642	MS.gene56245	0.864748	9.73E-65	-1.69E-46
MS.gene05470	MS.gene56245	0.823377	1.43E-53	-1.69E-46
MS.gene054954	MS.gene56245	0.836634	8.34E-57	-1.69E-46
MS.gene055206	MS.gene56245	0.856088	4.07E-62	-1.69E-46
MS.gene055212	MS.gene56245	0.805788	1.13E-49	-1.69E-46
MS.gene055289	MS.gene56245	0.803101	4.09E-49	-1.69E-46
MS.gene055362	MS.gene56245	0.849017	4.23E-60	-1.69E-46
MS.gene055502	MS.gene56245	0.84608	2.72E-59	-1.69E-46
MS.gene055577	MS.gene56245	0.845634	3.59E-59	-1.69E-46
MS.gene055722	MS.gene56245	0.80066	1.30E-48	-1.69E-46
MS.gene055748	MS.gene56245	0.839981	1.14E-57	-1.69E-46
MS.gene055751	MS.gene56245	0.832229	1.06E-55	-1.69E-46
MS.gene05589	MS.gene56245	0.814443	1.54E-51	-1.69E-46
MS.gene055941	MS.gene56245	0.814992	1.16E-51	-1.69E-46
MS.gene055956	MS.gene56245	-0.827283	1.70E-54	-1.69E-46
MS.gene056374	MS.gene56245	0.825105	5.62E-54	-1.69E-46
MS.gene056504	MS.gene56245	0.813088	3.05E-51	-1.69E-46
MS.gene056510	MS.gene56245	0.813797	2.13E-51	-1.69E-46
MS.gene056657	MS.gene56245	0.849412	3.29E-60	-1.69E-46
MS.gene056692	MS.gene56245	0.800846	1.19E-48	-1.69E-46
MS.gene056903	MS.gene56245	0.802634	5.11E-49	-1.69E-46
MS.gene057129	MS.gene56245	0.828998	6.58E-55	-1.69E-46
MS.gene057196	MS.gene56245	0.83762	4.67E-57	-1.69E-46
MS.gene057246	MS.gene56245	0.837384	5.37E-57	-1.69E-46
MS.gene057332	MS.gene56245	0.837455	5.15E-57	-1.69E-46
MS.gene057658	MS.gene56245	0.853773	1.91E-61	-1.69E-46
MS.gene057831	MS.gene56245	0.804291	2.32E-49	-1.69E-46
MS.gene058419	MS.gene56245	0.858328	8.88E-63	-1.69E-46
MS.gene058544	MS.gene56245	0.84053	8.23E-58	-1.69E-46
MS.gene058723	MS.gene56245	0.854828	9.49E-62	-1.69E-46
MS.gene058773	MS.gene56245	0.856135	3.95E-62	-1.69E-46
MS.gene058838	MS.gene56245	0.842381	2.68E-58	-1.69E-46
MS.gene059022	MS.gene56245	0.850889	1.27E-60	-1.69E-46
MS.gene059023	MS.gene56245	0.864645	1.05E-64	-1.69E-46
MS.gene059029	MS.gene56245	0.805905	1.06E-49	-1.69E-46
MS.gene05914	MS.gene56245	0.863531	2.33E-64	-1.69E-46
MS.gene059677	MS.gene56245	0.878939	1.87E-69	-1.69E-46
MS.gene059718	MS.gene56245	0.843135	1.69E-58	-1.69E-46
MS.gene059756	MS.gene56245	0.801343	9.42E-49	-1.69E-46
MS.gene059858	MS.gene56245	0.833643	4.74E-56	-1.69E-46
MS.gene059865	MS.gene56245	0.80557	1.25E-49	-1.69E-46
MS.gene059902	MS.gene56245	0.800477	1.42E-48	-1.69E-46
MS.gene059960	MS.gene56245	0.800745	1.25E-48	-1.69E-46
MS.gene060341	MS.gene56245	0.806514	7.93E-50	-1.69E-46
MS.gene060703	MS.gene56245	0.812276	4.60E-51	-1.69E-46
MS.gene060728	MS.gene56245	0.807787	4.26E-50	-1.69E-46
MS.gene060762	MS.gene56245	0.846649	1.90E-59	-1.69E-46
MS.gene06077	MS.gene56245	0.800627	1.32E-48	-1.69E-46
MS.gene06092	MS.gene56245	0.842125	3.13E-58	-1.69E-46
MS.gene060982	MS.gene56245	0.8037	3.08E-49	-1.69E-46
MS.gene061150	MS.gene56245	0.858279	9.19E-63	-1.69E-46
MS.gene061231	MS.gene56245	0.803031	4.23E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56245.t1	MTR_3g008920	98.106	264	5	0	1	264	1	264	0.0	522
MS.gene56245.t1	MTR_5g079570	75.000	244	59	1	5	248	19	260	9.18e-137	386
MS.gene56245.t1	MTR_4g084880	62.771	231	83	1	16	243	25	255	5.41e-106	308
MS.gene56245.t1	MTR_8g087720	53.279	244	111	2	16	258	28	269	4.10e-86	258
MS.gene56245.t1	MTR_2g017610	54.393	239	106	2	8	243	18	256	6.75e-86	257
MS.gene56245.t1	MTR_2g017570	53.975	239	107	2	8	243	18	256	7.43e-85	254
MS.gene56245.t1	MTR_2g017590	52.301	239	111	2	8	243	16	254	4.88e-83	250
MS.gene56245.t1	MTR_2g017660	48.425	254	125	3	8	258	19	269	1.26e-81	246
MS.gene56245.t1	MTR_8g087710	54.709	223	100	1	22	243	35	257	1.77e-81	246
MS.gene56245.t1	MTR_2g017620	50.628	239	115	2	8	243	4	242	1.87e-79	240
MS.gene56245.t1	MTR_2g017620	50.628	239	115	2	8	243	18	256	2.66e-79	240
MS.gene56245.t1	MTR_7g010650	48.558	208	103	3	26	231	37	242	2.08e-61	195
MS.gene56245.t1	MTR_2g017620	52.432	185	79	1	70	254	2	177	5.52e-61	191
MS.gene56245.t1	MTR_5g063930	46.188	223	98	3	25	235	36	248	1.01e-60	192
MS.gene56245.t1	MTR_4g006730	49.351	231	112	4	8	235	56	284	9.84e-60	191
MS.gene56245.t1	MTR_8g087720	52.299	174	82	1	16	188	28	201	4.53e-57	181
MS.gene56245.t1	MTR_2g104940	48.131	214	107	3	24	235	78	289	7.39e-57	184
MS.gene56245.t1	MTR_4g006630	50.251	199	95	3	39	235	1	197	1.20e-53	173
MS.gene56245.t1	MTR_4g006730	50.251	199	95	3	39	235	1	197	1.77e-53	172
MS.gene56245.t1	MTR_8g087720	53.595	153	70	1	17	168	29	181	8.36e-51	165
MS.gene56245.t1	MTR_1g097840	41.841	239	133	4	1	235	47	283	1.40e-50	168
MS.gene56245.t1	MTR_1g070385	38.117	223	136	2	14	235	50	271	6.32e-41	150
MS.gene56245.t1	MTR_4g006730	43.925	214	103	5	8	218	56	255	5.80e-38	134
MS.gene56245.t1	MTR_7g010650	45.890	146	75	3	26	169	37	180	4.17e-34	122
MS.gene56245.t1	MTR_1g077540	35.169	236	139	6	25	249	20	252	1.85e-30	114
MS.gene56245.t1	MTR_1g097840	39.583	192	110	4	1	188	47	236	2.95e-30	114
MS.gene56245.t1	MTR_2g101370	37.860	243	119	10	22	243	16	247	1.26e-28	109
MS.gene56245.t1	MTR_6g069040	33.898	236	143	6	25	251	20	251	1.22e-27	107
MS.gene56245.t1	MTR_8g013680	36.709	237	124	9	25	243	18	246	7.59e-27	104
MS.gene56245.t1	MTR_5g012810	35.983	239	134	7	25	253	18	247	2.37e-26	103
MS.gene56245.t1	MTR_1g006490	37.327	217	127	7	25	234	18	232	2.55e-26	103
MS.gene56245.t1	MTR_4g006650	48.951	143	60	4	27	167	5	136	1.58e-25	102
MS.gene56245.t1	MTR_1g115405	36.986	219	124	6	25	232	20	235	4.75e-24	97.4
MS.gene56245.t1	MTR_7g103030	35.345	232	125	9	30	243	25	249	1.09e-23	96.7
MS.gene56245.t1	MTR_1g006590	35.484	217	131	7	25	234	18	232	3.05e-23	95.5
MS.gene56245.t1	MTR_4g063090	37.383	214	118	8	31	232	26	235	2.28e-22	93.2
MS.gene56245.t1	MTR_3g118010	28.800	250	159	7	20	251	31	279	1.38e-21	91.7
MS.gene56245.t1	MTR_2g094270	33.862	189	111	5	59	234	84	271	4.22e-21	90.5
MS.gene56245.t1	MTR_7g023350	30.769	234	145	4	25	244	20	250	9.90e-20	85.9
MS.gene56245.t1	MTR_5g010150	31.771	192	118	4	54	232	84	275	2.97e-19	85.5
MS.gene56245.t1	MTR_7g101190	32.308	195	112	5	59	237	82	272	3.38e-19	85.1
MS.gene56245.t1	MTR_1g095070	31.343	201	117	6	67	250	24	220	1.86e-18	82.0
MS.gene56245.t1	MTR_5g082070	28.700	223	140	4	25	231	58	277	3.91e-18	82.4
MS.gene56245.t1	MTR_4g059390	30.208	192	114	5	59	234	82	269	1.17e-17	80.9
MS.gene56245.t1	MTR_8g098375	30.729	192	120	4	54	232	84	275	2.15e-17	80.1
MS.gene56245.t1	MTR_3g070210	29.101	189	115	4	59	231	92	277	1.16e-16	78.2
MS.gene56245.t1	MTR_2g060360	33.758	157	101	3	78	232	77	232	6.01e-16	75.5
MS.gene56245.t1	MTR_4g415300	27.803	223	137	7	30	232	60	278	1.28e-15	75.1
MS.gene56245.t1	MTR_2g094270	33.129	163	98	4	59	211	84	245	1.54e-15	74.7
MS.gene56245.t1	MTR_1g095070	32.026	153	87	4	67	206	24	172	1.76e-13	67.8
MS.gene56245.t1	MTR_3g070210	27.607	163	102	3	59	208	92	251	2.04e-12	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56245.t1	AT5G37820	62.083	240	81	2	16	254	33	263	2.45e-104	305
MS.gene56245.t1	AT5G37820	62.083	240	81	2	16	254	69	299	3.73e-104	305
MS.gene56245.t1	AT5G37810	60.246	244	94	2	16	258	33	274	1.26e-101	298
MS.gene56245.t1	AT4G18910	55.217	230	95	2	22	243	48	277	1.98e-84	254
MS.gene56245.t1	AT1G31885	47.581	248	120	3	22	261	39	284	2.63e-76	234
MS.gene56245.t1	AT4G19030	49.130	230	109	2	22	243	51	280	2.49e-74	229
MS.gene56245.t1	AT1G31885	48.707	232	109	3	38	261	1	230	3.83e-71	219
MS.gene56245.t1	AT2G34390	42.969	256	135	4	13	258	33	287	3.28e-67	210
MS.gene56245.t1	AT2G34390	43.333	240	127	3	27	258	7	245	4.99e-65	203
MS.gene56245.t1	AT4G18910	52.571	175	75	2	22	188	48	222	3.63e-56	180
MS.gene56245.t1	AT1G80760	44.651	215	117	2	22	235	76	289	1.34e-51	171
MS.gene56245.t1	AT4G10380	41.304	230	126	4	22	249	74	296	1.58e-48	163
MS.gene56245.t1	AT4G19030	45.402	174	87	2	22	187	51	224	5.56e-48	159
MS.gene56245.t1	AT2G34390	41.398	186	98	4	13	188	33	217	1.56e-43	147
MS.gene56245.t1	AT4G19030	46.452	155	75	2	22	168	51	205	5.93e-43	146
MS.gene56245.t1	AT2G34390	42.604	169	86	4	13	171	33	200	6.25e-40	138
MS.gene56245.t1	AT3G06100	35.321	218	138	3	25	240	45	261	4.00e-38	135
MS.gene56245.t1	AT3G06100	35.681	213	134	3	30	240	2	213	1.04e-37	132
MS.gene56245.t1	AT3G16240	35.776	232	132	8	25	245	18	243	1.57e-27	106
MS.gene56245.t1	AT3G26520	38.288	222	121	8	25	234	21	238	9.61e-27	104
MS.gene56245.t1	AT1G17810	34.348	230	124	6	25	234	23	245	1.24e-26	104
MS.gene56245.t1	AT1G73190	34.310	239	130	8	25	243	23	254	1.88e-26	104
MS.gene56245.t1	AT2G29870	50.526	95	47	0	142	236	22	116	3.85e-26	100
MS.gene56245.t1	AT2G25810	32.510	243	148	5	25	262	18	249	3.83e-25	100
MS.gene56245.t1	AT4G17340	32.751	229	145	4	25	244	18	246	6.78e-25	99.8
MS.gene56245.t1	AT5G47450	34.703	219	135	4	22	232	15	233	7.14e-25	99.8
MS.gene56245.t1	AT4G01470	33.333	231	116	6	25	232	20	235	3.35e-24	98.2
MS.gene56245.t1	AT2G16850	30.400	250	155	7	20	251	30	278	3.09e-23	95.9
MS.gene56245.t1	AT1G31885	45.122	82	44	1	22	102	39	120	1.32e-22	90.9
MS.gene56245.t1	AT2G36830	34.188	234	136	8	25	243	20	250	5.34e-22	92.0
MS.gene56245.t1	AT3G06100	34.752	141	90	2	101	240	41	180	7.16e-21	87.8
MS.gene56245.t1	AT1G01620	32.474	194	117	5	54	234	12	204	9.79e-20	85.1
MS.gene56245.t1	AT4G35100	32.812	192	115	5	59	237	75	265	1.10e-19	86.3
MS.gene56245.t1	AT4G35100	32.812	192	115	5	59	237	75	265	1.10e-19	86.3
MS.gene56245.t1	AT1G01620	32.474	194	117	5	54	234	84	276	1.93e-19	85.9
MS.gene56245.t1	AT5G60660	32.178	202	116	5	53	237	76	273	1.97e-19	85.9
MS.gene56245.t1	AT3G54820	32.308	195	112	5	59	237	81	271	2.98e-19	85.5
MS.gene56245.t1	AT3G53420	31.343	201	118	5	53	237	76	272	3.27e-19	85.1
MS.gene56245.t1	AT3G53420	31.343	201	118	5	53	237	76	272	3.27e-19	85.1
MS.gene56245.t1	AT4G00430	32.292	192	116	5	54	232	85	275	4.34e-19	84.7
MS.gene56245.t1	AT2G37170	31.343	201	118	5	53	237	74	270	1.26e-18	83.6
MS.gene56245.t1	AT2G37170	29.461	241	140	6	23	237	77	313	1.29e-18	84.3
MS.gene56245.t1	AT2G37180	29.046	241	141	6	23	237	34	270	4.80e-18	82.0
MS.gene56245.t1	AT3G47440	30.137	219	128	7	30	232	27	236	5.09e-18	81.3
MS.gene56245.t1	AT3G61430	31.606	193	114	5	54	231	84	273	6.38e-18	81.6
MS.gene56245.t1	AT3G61430	31.606	193	114	5	54	231	84	273	6.38e-18	81.6
MS.gene56245.t1	AT4G23400	31.034	203	128	4	54	244	85	287	6.79e-18	81.6
MS.gene56245.t1	AT2G39010	29.665	209	129	4	41	237	69	271	1.85e-17	80.5
MS.gene56245.t1	AT2G45960	30.570	193	116	5	54	231	84	273	7.21e-17	78.6
MS.gene56245.t1	AT2G45960	29.940	167	102	4	54	208	84	247	8.53e-13	67.0
MS.gene56245.t1	AT2G45960	29.940	167	102	4	54	208	84	247	9.82e-13	67.4

Find 56 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATTGTTGGTCCAATTAT+TGG	0.162167	3.3:-4477369	MS.gene56245:CDS
TTCATTGCTGGGCCTGTATC+AGG	0.233946	3.3:-4477462	MS.gene56245:CDS
CCAGCTATGGCTCCAATAAT+TGG	0.252991	3.3:+4477357	None:intergenic
TAAATATGTATTACAATTGC+AGG	0.326908	3.3:+4477408	None:intergenic
TACATTGTTGCTCAATTACT+TGG	0.343466	3.3:-4477702	MS.gene56245:CDS
TGCTGATCTTAGAAAGTTAT+AGG	0.344398	3.3:+4477326	None:intergenic
TTCCAGGGATTTGCATCACT+TGG	0.346536	3.3:-4477845	MS.gene56245:CDS
ATCACACCCGAATCTTATTT+CGG	0.354797	3.3:-4477639	MS.gene56245:CDS
GCAGGACCAATACTTCTTGC+TGG	0.368004	3.3:+4477426	None:intergenic
TTCATAGATGCTCCTGATAC+AGG	0.392349	3.3:+4477450	None:intergenic
TCCAGGGATTTGCATCACTT+GGG	0.394818	3.3:-4477844	MS.gene56245:CDS
AAGGTAACTGCAGAAGTTAT+TGG	0.400222	3.3:-4477936	MS.gene56245:CDS
CCAATTATTGGAGCCATAGC+TGG	0.416211	3.3:-4477357	MS.gene56245:CDS
TCTGCTATTGTCTCTGAAGT+TGG	0.422196	3.3:+4477297	None:intergenic
TTTCTAGTGTTTGCTGGTTG+TGG	0.424447	3.3:-4477906	MS.gene56245:CDS
ATCTTATTTCGGAACAGTAC+CGG	0.428414	3.3:-4477628	MS.gene56245:CDS
TACTGTTCCGAAATAAGATT+CGG	0.431345	3.3:+4477632	None:intergenic
CCAGGGATTTGCATCACTTG+GGG	0.433980	3.3:-4477843	MS.gene56245:CDS
ACAGTACCGGTCGGATCAAA+TGG	0.438105	3.3:-4477615	MS.gene56245:CDS
TACTCCGTTGGTCATATCTC+TGG	0.449191	3.3:-4477798	MS.gene56245:CDS
TGAAACATTGTTGTTCATCA+AGG	0.453880	3.3:-4477980	MS.gene56245:CDS
TCTGCTGTTAATTCTGAAGT+TGG	0.471889	3.3:+4477270	None:intergenic
ATGAACAACAATGTTTCAAG+AGG	0.474822	3.3:+4477985	None:intergenic
TGTAATAACCTTGATACAAA+AGG	0.477490	3.3:-4477955	MS.gene56245:CDS
AAATGGTCAATCTTTAGTGT+TGG	0.484202	3.3:-4477598	MS.gene56245:CDS
ACTGTTCCGAAATAAGATTC+GGG	0.493250	3.3:+4477633	None:intergenic
ACCCTGCTGTTACAATCACC+TGG	0.496483	3.3:-4477764	MS.gene56245:CDS
TGATAGAGCGGTCGATGACT+CGG	0.499012	3.3:-4477523	MS.gene56245:CDS
GCCCAGGTGATTGTAACAGC+AGG	0.500454	3.3:+4477762	None:intergenic
TCGTCGCATCACGATTAAAG+AGG	0.508434	3.3:-4477733	MS.gene56245:CDS
ATGAATCCAGCAAGAAGTAT+TGG	0.512100	3.3:-4477432	MS.gene56245:CDS
CCCCAAGTGATGCAAATCCC+TGG	0.519528	3.3:+4477843	None:intergenic
GGATTGTGGATATATATTGT+TGG	0.522254	3.3:-4477381	MS.gene56245:CDS
TACATATTTACAAAGGATTG+TGG	0.529405	3.3:-4477395	MS.gene56245:CDS
ACATTGTTGCTCAATTACTT+GGG	0.530403	3.3:-4477701	MS.gene56245:CDS
CAGGGATTTGCATCACTTGG+GGG	0.538642	3.3:-4477842	MS.gene56245:CDS
GACTCGGCAAGTATTGCAGT+TGG	0.544686	3.3:-4477507	MS.gene56245:CDS
ATAGGCAAGTGCTCCAGCTA+TGG	0.547527	3.3:+4477344	None:intergenic
GATTGACCATTTGATCCGAC+CGG	0.564200	3.3:+4477609	None:intergenic
TCAATGGCAATAAAACATCA+AGG	0.580804	3.3:-4478017	MS.gene56245:CDS
TCCGTTGGTCATATCTCTGG+AGG	0.584856	3.3:-4477795	MS.gene56245:CDS
ATTGTAATACATATTTACAA+AGG	0.588504	3.3:-4477402	MS.gene56245:CDS
TTGTAACAGCAGGGTTGAAG+AGG	0.589344	3.3:+4477772	None:intergenic
ACAGTCATGTGTTACTCCGT+TGG	0.600056	3.3:-4477810	MS.gene56245:CDS
CCCTGCTGTTACAATCACCT+GGG	0.604644	3.3:-4477763	MS.gene56245:CDS
ACCTACACAATGTTCTCTGA+AGG	0.609197	3.3:+4477240	None:intergenic
CCCAGGTGATTGTAACAGCA+GGG	0.612416	3.3:+4477763	None:intergenic
TACACAATGTTCTCTGAAGG+TGG	0.619523	3.3:+4477243	None:intergenic
GTAGCAATAAATAAGATACA+TGG	0.620958	3.3:-4477879	MS.gene56245:CDS
TGAACAACAATGTTTCAAGA+GGG	0.621482	3.3:+4477986	None:intergenic
GCCTCCAGAGATATGACCAA+CGG	0.643828	3.3:+4477794	None:intergenic
GATGCGACGAAAGATAGCCC+AGG	0.654187	3.3:+4477746	None:intergenic
AATTGAGCAACAATGTACAA+AGG	0.662807	3.3:+4477708	None:intergenic
ACCTTCAGAGAACATTGTGT+AGG	0.675859	3.3:-4477241	None:intergenic
TATTTCGGAACAGTACCGGT+CGG	0.685297	3.3:-4477624	MS.gene56245:CDS
TGTTACTACAGATGATAGAG+CGG	0.715844	3.3:-4477535	MS.gene56245:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGTAATACATATTTACAA+AGG	-	chr3.3:4477854-4477873	MS.gene56245:CDS	15.0%
!!	TAAATATGTATTACAATTGC+AGG	+	chr3.3:4477851-4477870	None:intergenic	20.0%
!	GTAGCAATAAATAAGATACA+TGG	-	chr3.3:4477377-4477396	MS.gene56245:CDS	25.0%
!	TACATATTTACAAAGGATTG+TGG	-	chr3.3:4477861-4477880	MS.gene56245:CDS	25.0%
!	TATATTGTTGGTCCAATTAT+TGG	-	chr3.3:4477887-4477906	MS.gene56245:CDS	25.0%
!	TGTAATAACCTTGATACAAA+AGG	-	chr3.3:4477301-4477320	MS.gene56245:CDS	25.0%
	AAATGGTCAATCTTTAGTGT+TGG	-	chr3.3:4477658-4477677	MS.gene56245:CDS	30.0%
	AATTGAGCAACAATGTACAA+AGG	+	chr3.3:4477551-4477570	None:intergenic	30.0%
	ACATTGTTGCTCAATTACTT+GGG	-	chr3.3:4477555-4477574	MS.gene56245:CDS	30.0%
	ATGAACAACAATGTTTCAAG+AGG	+	chr3.3:4477274-4477293	None:intergenic	30.0%
	GGATTGTGGATATATATTGT+TGG	-	chr3.3:4477875-4477894	MS.gene56245:CDS	30.0%
	TACATTGTTGCTCAATTACT+TGG	-	chr3.3:4477554-4477573	MS.gene56245:CDS	30.0%
	TACTGTTCCGAAATAAGATT+CGG	+	chr3.3:4477627-4477646	None:intergenic	30.0%
	TGAACAACAATGTTTCAAGA+GGG	+	chr3.3:4477273-4477292	None:intergenic	30.0%
	TGCTGATCTTAGAAAGTTAT+AGG	+	chr3.3:4477933-4477952	None:intergenic	30.0%
!	ACATTGAATCTTTTCATTGC+TGG	-	chr3.3:4477782-4477801	MS.gene56245:CDS	30.0%
!	CATTGAATCTTTTCATTGCT+GGG	-	chr3.3:4477783-4477802	MS.gene56245:CDS	30.0%
!	TGAAACATTGTTGTTCATCA+AGG	-	chr3.3:4477276-4477295	MS.gene56245:CDS	30.0%
!!	ACATATTTTCTAGTGTTTGC+TGG	-	chr3.3:4477344-4477363	MS.gene56245:CDS	30.0%
	AAGGTAACTGCAGAAGTTAT+TGG	-	chr3.3:4477320-4477339	MS.gene56245:CDS	35.0%
	ACTGTTCCGAAATAAGATTC+GGG	+	chr3.3:4477626-4477645	None:intergenic	35.0%
	TGTTACTACAGATGATAGAG+CGG	-	chr3.3:4477721-4477740	MS.gene56245:CDS	35.0%
!	ATCACACCCGAATCTTATTT+CGG	-	chr3.3:4477617-4477636	MS.gene56245:CDS	35.0%
!	ATCTTATTTCGGAACAGTAC+CGG	-	chr3.3:4477628-4477647	MS.gene56245:CDS	35.0%
!	ATGAATCCAGCAAGAAGTAT+TGG	-	chr3.3:4477824-4477843	MS.gene56245:CDS	35.0%
!	TGCAGTTACCTTTTGTATCA+AGG	+	chr3.3:4477312-4477331	None:intergenic	35.0%
!!	TCTGCTGTTAATTCTGAAGT+TGG	+	chr3.3:4477989-4478008	None:intergenic	35.0%
	TTCATAGATGCTCCTGATAC+AGG	+	chr3.3:4477809-4477828	None:intergenic	40.0%
!	TACACAATGTTCTCTGAAGG+TGG	+	chr3.3:4478016-4478035	None:intergenic	40.0%
!	TTTCTAGTGTTTGCTGGTTG+TGG	-	chr3.3:4477350-4477369	MS.gene56245:CDS	40.0%
!!	ATGGCTCTATCACTTTTCCA+GGG	-	chr3.3:4477396-4477415	MS.gene56245:CDS	40.0%
!!	TCTGCTATTGTCTCTGAAGT+TGG	+	chr3.3:4477962-4477981	None:intergenic	40.0%
	CCAATTATTGGAGCCATAGC+TGG	-	chr3.3:4477899-4477918	MS.gene56245:CDS	45.0%
	CCAGCTATGGCTCCAATAAT+TGG	+	chr3.3:4477902-4477921	None:intergenic	45.0%
	GATTGACCATTTGATCCGAC+CGG	+	chr3.3:4477650-4477669	None:intergenic	45.0%
	TACTCCGTTGGTCATATCTC+TGG	-	chr3.3:4477458-4477477	MS.gene56245:CDS	45.0%
	TCCAGGGATTTGCATCACTT+GGG	-	chr3.3:4477412-4477431	MS.gene56245:CDS	45.0%
	TCGTCGCATCACGATTAAAG+AGG	-	chr3.3:4477523-4477542	MS.gene56245:CDS	45.0%
	TTCCAGGGATTTGCATCACT+TGG	-	chr3.3:4477411-4477430	MS.gene56245:CDS	45.0%
	TTGTAACAGCAGGGTTGAAG+AGG	+	chr3.3:4477487-4477506	None:intergenic	45.0%
!	ACAGTCATGTGTTACTCCGT+TGG	-	chr3.3:4477446-4477465	MS.gene56245:CDS	45.0%
!!	CATGGCTCTATCACTTTTCC+AGG	-	chr3.3:4477395-4477414	MS.gene56245:CDS	45.0%
!!	TATTTCGGAACAGTACCGGT+CGG	-	chr3.3:4477632-4477651	MS.gene56245:CDS	45.0%
	ACCCTGCTGTTACAATCACC+TGG	-	chr3.3:4477492-4477511	MS.gene56245:CDS	50.0%
	ATAGGCAAGTGCTCCAGCTA+TGG	+	chr3.3:4477915-4477934	None:intergenic	50.0%
	CAGGGATTTGCATCACTTGG+GGG	-	chr3.3:4477414-4477433	MS.gene56245:CDS	50.0%
	CCAGGGATTTGCATCACTTG+GGG	-	chr3.3:4477413-4477432	MS.gene56245:CDS	50.0%
	CCCAGGTGATTGTAACAGCA+GGG	+	chr3.3:4477496-4477515	None:intergenic	50.0%
	CCCTGCTGTTACAATCACCT+GGG	-	chr3.3:4477493-4477512	MS.gene56245:CDS	50.0%
	GCAGGACCAATACTTCTTGC+TGG	+	chr3.3:4477833-4477852	None:intergenic	50.0%
	GCCTCCAGAGATATGACCAA+CGG	+	chr3.3:4477465-4477484	None:intergenic	50.0%
	TCCGTTGGTCATATCTCTGG+AGG	-	chr3.3:4477461-4477480	MS.gene56245:CDS	50.0%
	TGATAGAGCGGTCGATGACT+CGG	-	chr3.3:4477733-4477752	MS.gene56245:CDS	50.0%
	TTCATTGCTGGGCCTGTATC+AGG	-	chr3.3:4477794-4477813	MS.gene56245:CDS	50.0%
!	GACTCGGCAAGTATTGCAGT+TGG	-	chr3.3:4477749-4477768	MS.gene56245:CDS	50.0%
!!	ACAGTACCGGTCGGATCAAA+TGG	-	chr3.3:4477641-4477660	MS.gene56245:CDS	50.0%
	CCCCAAGTGATGCAAATCCC+TGG	+	chr3.3:4477416-4477435	None:intergenic	55.0%
	GATGCGACGAAAGATAGCCC+AGG	+	chr3.3:4477513-4477532	None:intergenic	55.0%
	GCCCAGGTGATTGTAACAGC+AGG	+	chr3.3:4477497-4477516	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	4477242	4478036	4477242	ID=MS.gene56245
chr3.3	mRNA	4477242	4478036	4477242	ID=MS.gene56245.t1;Parent=MS.gene56245
chr3.3	exon	4477242	4478036	4477242	ID=MS.gene56245.t1.exon1;Parent=MS.gene56245.t1
chr3.3	CDS	4477242	4478036	4477242	ID=cds.MS.gene56245.t1;Parent=MS.gene56245.t1

Gene Sequence

>MS.gene56245

ATGGCAATAAAACATCAAGGAAACAATTCCCTCTTGAAACATTGTTGTTCATCAAGGCGTGTAATAACCTTGATACAAAAGGTAACTGCAGAAGTTATTGGTACATATTTTCTAGTGTTTGCTGGTTGTGGTGCTGTAGCAATAAATAAGATACATGGCTCTATCACTTTTCCAGGGATTTGCATCACTTGGGGGTTGATTGTAACAGTCATGTGTTACTCCGTTGGTCATATCTCTGGAGGCCTCTTCAACCCTGCTGTTACAATCACCTGGGCTATCTTTCGTCGCATCACGATTAAAGAGGCTCCTTTGTACATTGTTGCTCAATTACTTGGGTCTACACTTGCAAGTGTAACATTATCTCTAATGTTTGATATCACACCCGAATCTTATTTCGGAACAGTACCGGTCGGATCAAATGGTCAATCTTTAGTGTTGGAAATCATTATCAGTTTTCTCTTAATGTTTGTCATTTCAGCTGTTACTACAGATGATAGAGCGGTCGATGACTCGGCAAGTATTGCAGTTGGAATGACTTTAACATTGAATCTTTTCATTGCTGGGCCTGTATCAGGAGCATCTATGAATCCAGCAAGAAGTATTGGTCCTGCAATTGTAATACATATTTACAAAGGATTGTGGATATATATTGTTGGTCCAATTATTGGAGCCATAGCTGGAGCACTTGCCTATAACTTTCTAAGATCAGCATACAAGCCAACTTCAGAGACAATAGCAGACAAACCAACTTCAGAATTAACAGCAGACAAGCCACCTTCAGAGAACATTGTGTAG

Protein sequence

>MS.gene56245.t1

MAIKHQGNNSLLKHCCSSRRVITLIQKVTAEVIGTYFLVFAGCGAVAINKIHGSITFPGICITWGLIVTVMCYSVGHISGGLFNPAVTITWAIFRRITIKEAPLYIVAQLLGSTLASVTLSLMFDITPESYFGTVPVGSNGQSLVLEIIISFLLMFVISAVTTDDRAVDDSASIAVGMTLTLNLFIAGPVSGASMNPARSIGPAIVIHIYKGLWIYIVGPIIGAIAGALAYNFLRSAYKPTSETIADKPTSELTADKPPSENIV