Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63040.t1 | XP_003614634.1 | 99.3 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 5.70E-265 | 922.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63040.t1 | Q9XF43 | 82.8 | 460 | 79 | 0 | 1 | 460 | 38 | 497 | 3.3e-229 | 795.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63040.t1 | G7K1J8 | 99.3 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 4.1e-265 | 922.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene63040 | 0.814408 | 1.56E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene63040 | 0.807321 | 5.35E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene63040 | 0.832659 | 8.33E-56 | -1.69E-46 |
| MS.gene049369 | MS.gene63040 | 0.849331 | 3.46E-60 | -1.69E-46 |
| MS.gene049396 | MS.gene63040 | 0.826484 | 2.65E-54 | -1.69E-46 |
| MS.gene049609 | MS.gene63040 | 0.801854 | 7.40E-49 | -1.69E-46 |
| MS.gene049828 | MS.gene63040 | 0.819205 | 1.31E-52 | -1.69E-46 |
| MS.gene049907 | MS.gene63040 | 0.882737 | 8.11E-71 | -1.69E-46 |
| MS.gene050499 | MS.gene63040 | 0.810253 | 1.26E-50 | -1.69E-46 |
| MS.gene050502 | MS.gene63040 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene050520 | MS.gene63040 | 0.866708 | 2.34E-65 | -1.69E-46 |
| MS.gene050593 | MS.gene63040 | 0.809457 | 1.87E-50 | -1.69E-46 |
| MS.gene050662 | MS.gene63040 | 0.826804 | 2.22E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene63040 | 0.814858 | 1.24E-51 | -1.69E-46 |
| MS.gene050686 | MS.gene63040 | 0.855624 | 5.57E-62 | -1.69E-46 |
| MS.gene050767 | MS.gene63040 | 0.812937 | 3.30E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene63040 | 0.812955 | 3.27E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene63040 | 0.829793 | 4.22E-55 | -1.69E-46 |
| MS.gene05105 | MS.gene63040 | 0.826961 | 2.04E-54 | -1.69E-46 |
| MS.gene051255 | MS.gene63040 | 0.840933 | 6.45E-58 | -1.69E-46 |
| MS.gene051387 | MS.gene63040 | 0.820505 | 6.62E-53 | -1.69E-46 |
| MS.gene051509 | MS.gene63040 | 0.854725 | 1.02E-61 | -1.69E-46 |
| MS.gene051692 | MS.gene63040 | 0.812539 | 4.03E-51 | -1.69E-46 |
| MS.gene051858 | MS.gene63040 | 0.810476 | 1.13E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene63040 | 0.807849 | 4.13E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene63040 | 0.877946 | 4.18E-69 | -1.69E-46 |
| MS.gene052421 | MS.gene63040 | 0.827453 | 1.55E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene63040 | 0.802986 | 4.32E-49 | -1.69E-46 |
| MS.gene052999 | MS.gene63040 | 0.818495 | 1.90E-52 | -1.69E-46 |
| MS.gene053018 | MS.gene63040 | 0.828099 | 1.08E-54 | -1.69E-46 |
| MS.gene053213 | MS.gene63040 | 0.819 | 1.46E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene63040 | 0.81542 | 9.33E-52 | -1.69E-46 |
| MS.gene053322 | MS.gene63040 | 0.807987 | 3.86E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene63040 | 0.818656 | 1.75E-52 | -1.69E-46 |
| MS.gene053505 | MS.gene63040 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene63040 | 0.829913 | 3.94E-55 | -1.69E-46 |
| MS.gene053813 | MS.gene63040 | 0.940221 | 3.38E-100 | -1.69E-46 |
| MS.gene053912 | MS.gene63040 | 0.814901 | 1.22E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene63040 | 0.803737 | 3.02E-49 | -1.69E-46 |
| MS.gene054486 | MS.gene63040 | 0.806499 | 7.99E-50 | -1.69E-46 |
| MS.gene054595 | MS.gene63040 | 0.80871 | 2.71E-50 | -1.69E-46 |
| MS.gene05492 | MS.gene63040 | 0.806316 | 8.73E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene63040 | 0.846034 | 2.80E-59 | -1.69E-46 |
| MS.gene055397 | MS.gene63040 | 0.808912 | 2.45E-50 | -1.69E-46 |
| MS.gene055647 | MS.gene63040 | 0.853937 | 1.72E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene63040 | 0.844069 | 9.50E-59 | -1.69E-46 |
| MS.gene055802 | MS.gene63040 | 0.82434 | 8.51E-54 | -1.69E-46 |
| MS.gene055909 | MS.gene63040 | 0.842706 | 2.19E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene63040 | 0.844492 | 7.31E-59 | -1.69E-46 |
| MS.gene05592 | MS.gene63040 | 0.833569 | 4.95E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene63040 | 0.801175 | 1.02E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene63040 | 0.831678 | 1.46E-55 | -1.69E-46 |
| MS.gene056374 | MS.gene63040 | 0.813856 | 2.07E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene63040 | 0.81024 | 1.27E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene63040 | 0.850752 | 1.39E-60 | -1.69E-46 |
| MS.gene056511 | MS.gene63040 | 0.828025 | 1.13E-54 | -1.69E-46 |
| MS.gene056611 | MS.gene63040 | 0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene63040 | 0.824423 | 8.14E-54 | -1.69E-46 |
| MS.gene056942 | MS.gene63040 | 0.869142 | 3.86E-66 | -1.69E-46 |
| MS.gene057076 | MS.gene63040 | 0.829336 | 5.45E-55 | -1.69E-46 |
| MS.gene057129 | MS.gene63040 | 0.800958 | 1.13E-48 | -1.69E-46 |
| MS.gene057146 | MS.gene63040 | 0.809634 | 1.72E-50 | -1.69E-46 |
| MS.gene057285 | MS.gene63040 | 0.819215 | 1.31E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene63040 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene63040 | 0.828258 | 9.93E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene63040 | 0.820439 | 6.86E-53 | -1.69E-46 |
| MS.gene057749 | MS.gene63040 | 0.824665 | 7.14E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene63040 | 0.828073 | 1.10E-54 | -1.69E-46 |
| MS.gene057926 | MS.gene63040 | 0.827835 | 1.26E-54 | -1.69E-46 |
| MS.gene058019 | MS.gene63040 | 0.83459 | 2.75E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene63040 | 0.804131 | 2.50E-49 | -1.69E-46 |
| MS.gene058459 | MS.gene63040 | 0.868493 | 6.27E-66 | -1.69E-46 |
| MS.gene058660 | MS.gene63040 | 0.804856 | 1.77E-49 | -1.69E-46 |
| MS.gene058903 | MS.gene63040 | 0.849513 | 3.08E-60 | -1.69E-46 |
| MS.gene058995 | MS.gene63040 | 0.827407 | 1.59E-54 | -1.69E-46 |
| MS.gene05913 | MS.gene63040 | 0.820308 | 7.35E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene63040 | 0.84884 | 4.74E-60 | -1.69E-46 |
| MS.gene059368 | MS.gene63040 | 0.857448 | 1.62E-62 | -1.69E-46 |
| MS.gene059455 | MS.gene63040 | 0.814529 | 1.47E-51 | -1.69E-46 |
| MS.gene059536 | MS.gene63040 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene63040 | 0.844155 | 9.01E-59 | -1.69E-46 |
| MS.gene059680 | MS.gene63040 | 0.832516 | 9.04E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene63040 | 0.808827 | 2.56E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene63040 | 0.805126 | 1.55E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene63040 | 0.89383 | 4.36E-75 | -1.69E-46 |
| MS.gene059807 | MS.gene63040 | 0.806148 | 9.47E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene63040 | 0.802945 | 4.41E-49 | -1.69E-46 |
| MS.gene059974 | MS.gene63040 | 0.83697 | 6.85E-57 | -1.69E-46 |
| MS.gene060005 | MS.gene63040 | 0.836561 | 8.71E-57 | -1.69E-46 |
| MS.gene060356 | MS.gene63040 | 0.839494 | 1.53E-57 | -1.69E-46 |
| MS.gene060482 | MS.gene63040 | 0.832044 | 1.18E-55 | -1.69E-46 |
| MS.gene060801 | MS.gene63040 | 0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene63040 | 0.844526 | 7.16E-59 | -1.69E-46 |
| MS.gene060818 | MS.gene63040 | 0.812422 | 4.27E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene63040 | 0.818088 | 2.35E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene63040 | 0.862532 | 4.74E-64 | -1.69E-46 |
| MS.gene061041 | MS.gene63040 | 0.817489 | 3.21E-52 | -1.69E-46 |
| MS.gene061134 | MS.gene63040 | 0.831167 | 1.95E-55 | -1.69E-46 |
| MS.gene061200 | MS.gene63040 | 0.855534 | 5.92E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63040.t1 | MTR_5g056510 | 99.348 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 0.0 | 951 |
| MS.gene63040.t1 | MTR_1g110930 | 90.000 | 460 | 46 | 0 | 1 | 460 | 37 | 496 | 0.0 | 860 |
| MS.gene63040.t1 | MTR_4g070270 | 64.758 | 454 | 160 | 0 | 1 | 454 | 49 | 502 | 0.0 | 637 |
| MS.gene63040.t1 | MTR_1g041085 | 62.640 | 447 | 166 | 1 | 15 | 460 | 72 | 518 | 0.0 | 597 |
| MS.gene63040.t1 | MTR_1g076430 | 61.521 | 447 | 171 | 1 | 15 | 460 | 66 | 512 | 0.0 | 593 |
| MS.gene63040.t1 | MTR_2g054940 | 62.613 | 444 | 165 | 1 | 15 | 457 | 65 | 508 | 0.0 | 592 |
| MS.gene63040.t1 | MTR_7g118170 | 60.486 | 453 | 170 | 4 | 9 | 455 | 80 | 529 | 0.0 | 565 |
| MS.gene63040.t1 | MTR_4g113280 | 59.326 | 445 | 167 | 3 | 15 | 457 | 54 | 486 | 0.0 | 562 |
| MS.gene63040.t1 | MTR_7g081695 | 59.513 | 452 | 170 | 2 | 1 | 451 | 51 | 490 | 0.0 | 555 |
| MS.gene63040.t1 | MTR_3g105550 | 55.034 | 447 | 190 | 3 | 19 | 454 | 90 | 536 | 0.0 | 530 |
| MS.gene63040.t1 | MTR_2g007020 | 54.015 | 411 | 185 | 3 | 47 | 455 | 44 | 452 | 1.44e-168 | 482 |
| MS.gene63040.t1 | MTR_4g054630 | 52.214 | 429 | 201 | 3 | 30 | 455 | 40 | 467 | 9.19e-159 | 457 |
| MS.gene63040.t1 | MTR_4g054415 | 52.594 | 424 | 197 | 3 | 34 | 454 | 3 | 425 | 1.48e-158 | 456 |
| MS.gene63040.t1 | MTR_6g074880 | 52.125 | 447 | 208 | 4 | 13 | 456 | 27 | 470 | 7.71e-158 | 455 |
| MS.gene63040.t1 | MTR_2g436480 | 50.353 | 425 | 207 | 3 | 33 | 454 | 28 | 451 | 2.00e-157 | 454 |
| MS.gene63040.t1 | MTR_1g102290 | 52.334 | 407 | 192 | 2 | 48 | 452 | 78 | 484 | 1.50e-156 | 452 |
| MS.gene63040.t1 | MTR_2g013740 | 53.671 | 395 | 180 | 1 | 49 | 443 | 109 | 500 | 2.63e-154 | 447 |
| MS.gene63040.t1 | MTR_7g118160 | 52.696 | 408 | 148 | 4 | 52 | 456 | 56 | 421 | 4.35e-153 | 442 |
| MS.gene63040.t1 | MTR_3g072980 | 51.582 | 411 | 196 | 3 | 44 | 452 | 74 | 483 | 8.65e-151 | 438 |
| MS.gene63040.t1 | MTR_3g072940 | 51.582 | 411 | 196 | 3 | 44 | 452 | 74 | 483 | 9.13e-151 | 438 |
| MS.gene63040.t1 | MTR_6g032965 | 52.815 | 373 | 175 | 1 | 84 | 455 | 13 | 385 | 8.65e-147 | 424 |
| MS.gene63040.t1 | MTR_6g051440 | 51.117 | 403 | 194 | 3 | 52 | 452 | 81 | 482 | 1.59e-138 | 407 |
| MS.gene63040.t1 | MTR_1g115560 | 39.394 | 429 | 230 | 6 | 48 | 451 | 28 | 451 | 3.87e-109 | 332 |
| MS.gene63040.t1 | MTR_4g062270 | 40.094 | 424 | 242 | 6 | 34 | 451 | 16 | 433 | 7.97e-109 | 330 |
| MS.gene63040.t1 | MTR_2g096210 | 40.732 | 437 | 236 | 8 | 34 | 453 | 16 | 446 | 1.52e-107 | 327 |
| MS.gene63040.t1 | MTR_4g062190 | 39.269 | 438 | 235 | 8 | 34 | 451 | 16 | 442 | 3.27e-106 | 323 |
| MS.gene63040.t1 | MTR_4g062170 | 40.046 | 432 | 239 | 7 | 34 | 451 | 16 | 441 | 9.92e-105 | 320 |
| MS.gene63040.t1 | MTR_4g054260 | 49.805 | 257 | 108 | 4 | 201 | 454 | 87 | 325 | 1.49e-78 | 248 |
| MS.gene63040.t1 | MTR_4g053905 | 56.875 | 160 | 66 | 2 | 297 | 454 | 1 | 159 | 6.74e-59 | 191 |
| MS.gene63040.t1 | MTR_7g086260 | 69.355 | 62 | 17 | 1 | 292 | 351 | 22 | 83 | 4.31e-19 | 82.0 |
| MS.gene63040.t1 | MTR_4g054265 | 37.069 | 116 | 73 | 0 | 34 | 149 | 3 | 118 | 8.69e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63040.t1 | AT1G68530 | 82.826 | 460 | 79 | 0 | 1 | 460 | 38 | 497 | 0.0 | 822 |
| MS.gene63040.t1 | AT1G25450 | 80.963 | 457 | 87 | 0 | 4 | 460 | 36 | 492 | 0.0 | 805 |
| MS.gene63040.t1 | AT1G19440 | 63.636 | 451 | 164 | 0 | 2 | 452 | 62 | 512 | 0.0 | 635 |
| MS.gene63040.t1 | AT2G16280 | 64.317 | 454 | 162 | 0 | 1 | 454 | 57 | 510 | 0.0 | 632 |
| MS.gene63040.t1 | AT4G34510 | 60.475 | 463 | 174 | 3 | 1 | 454 | 23 | 485 | 0.0 | 588 |
| MS.gene63040.t1 | AT2G26640 | 60.135 | 444 | 176 | 1 | 15 | 457 | 63 | 506 | 0.0 | 575 |
| MS.gene63040.t1 | AT1G04220 | 60.771 | 441 | 165 | 3 | 23 | 455 | 76 | 516 | 0.0 | 562 |
| MS.gene63040.t1 | AT5G43760 | 60.938 | 448 | 166 | 4 | 19 | 458 | 78 | 524 | 0.0 | 560 |
| MS.gene63040.t1 | AT1G01120 | 58.447 | 438 | 180 | 1 | 21 | 456 | 91 | 528 | 0.0 | 552 |
| MS.gene63040.t1 | AT1G68530 | 76.292 | 329 | 72 | 1 | 1 | 329 | 38 | 360 | 0.0 | 535 |
| MS.gene63040.t1 | AT2G15090 | 57.009 | 428 | 176 | 2 | 35 | 454 | 52 | 479 | 0.0 | 533 |
| MS.gene63040.t1 | AT4G34250 | 53.043 | 460 | 210 | 1 | 1 | 454 | 28 | 487 | 0.0 | 519 |
| MS.gene63040.t1 | AT4G34520 | 53.728 | 456 | 200 | 2 | 10 | 454 | 35 | 490 | 0.0 | 518 |
| MS.gene63040.t1 | AT2G26250 | 50.833 | 480 | 206 | 4 | 2 | 452 | 70 | 548 | 4.36e-179 | 512 |
| MS.gene63040.t1 | AT2G46720 | 53.623 | 414 | 189 | 1 | 44 | 454 | 52 | 465 | 5.06e-174 | 496 |
| MS.gene63040.t1 | AT3G10280 | 52.174 | 414 | 188 | 2 | 44 | 454 | 52 | 458 | 4.73e-166 | 476 |
| MS.gene63040.t1 | AT1G71160 | 49.652 | 431 | 213 | 3 | 23 | 452 | 19 | 446 | 1.30e-162 | 467 |
| MS.gene63040.t1 | AT5G49070 | 51.054 | 427 | 198 | 3 | 37 | 456 | 35 | 457 | 6.44e-153 | 442 |
| MS.gene63040.t1 | AT3G52160 | 45.301 | 415 | 195 | 3 | 30 | 443 | 66 | 449 | 6.52e-132 | 389 |
| MS.gene63040.t1 | AT3G52160 | 47.922 | 361 | 156 | 3 | 84 | 443 | 7 | 336 | 6.55e-123 | 362 |
| MS.gene63040.t1 | AT2G28630 | 42.080 | 423 | 223 | 8 | 48 | 451 | 27 | 446 | 2.19e-110 | 334 |
| MS.gene63040.t1 | AT2G28630 | 42.080 | 423 | 223 | 8 | 48 | 451 | 27 | 446 | 2.19e-110 | 334 |
| MS.gene63040.t1 | AT5G04530 | 40.777 | 412 | 229 | 6 | 50 | 451 | 31 | 437 | 1.20e-107 | 327 |
| MS.gene63040.t1 | AT1G07720 | 40.811 | 419 | 225 | 6 | 53 | 451 | 32 | 447 | 1.07e-106 | 325 |
| MS.gene63040.t1 | AT1G07720 | 40.811 | 419 | 225 | 6 | 53 | 451 | 75 | 490 | 1.64e-106 | 326 |
| MS.gene63040.t1 | AT5G49070 | 48.387 | 279 | 143 | 1 | 37 | 314 | 35 | 313 | 1.07e-88 | 273 |
Find 86 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACATCTTCTTCATCTTTA+TGG | 0.117063 | 5.4:-44782596 | MS.gene63040:CDS |
| CGTCCCTTTCGCTACTTTCA+TGG | 0.215185 | 5.4:-44783558 | MS.gene63040:CDS |
| TTCACTTCAAAAGGATCTTA+TGG | 0.218445 | 5.4:-44782889 | MS.gene63040:CDS |
| CGTCTTGCTTCTTCCATTGT+TGG | 0.258187 | 5.4:+44783410 | None:intergenic |
| AGCATTAAAACACCTGTTAA+TGG | 0.264447 | 5.4:-44782459 | MS.gene63040:CDS |
| GCTTCTTCCATTGTTGGTTT+AGG | 0.276327 | 5.4:+44783416 | None:intergenic |
| TGAAGAGAGGTGATAGAGTA+TGG | 0.287194 | 5.4:-44782539 | MS.gene63040:CDS |
| TGTTTGAAATCAGGAATATA+AGG | 0.323168 | 5.4:+44782741 | None:intergenic |
| GGTGGGATGTAATGGATTGC+TGG | 0.333428 | 5.4:+44783437 | None:intergenic |
| GAAGACAGAGAAGGTAAAGT+TGG | 0.348142 | 5.4:-44782915 | MS.gene63040:CDS |
| GAATGACGCTGCATCGATTC+GGG | 0.353166 | 5.4:-44782620 | MS.gene63040:CDS |
| ACAACAACTGCGTTTGAATT+CGG | 0.360073 | 5.4:+44783143 | None:intergenic |
| AGAGTATGGCAAATAGCTTT+TGG | 0.363430 | 5.4:-44782525 | MS.gene63040:CDS |
| ATCGTTCGAGAATTCTCATT+TGG | 0.366002 | 5.4:+44783489 | None:intergenic |
| AGCTTCAACCTCTCCGGAAT+GGG | 0.368697 | 5.4:-44783221 | MS.gene63040:CDS |
| TTTAGCCCGCGATCTTCTTC+AGG | 0.368914 | 5.4:-44783171 | MS.gene63040:CDS |
| ACCAAACTGTTTATTCAGAA+TGG | 0.377309 | 5.4:-44783063 | MS.gene63040:CDS |
| AAATCAAATATAACAACTAT+TGG | 0.388103 | 5.4:-44782846 | MS.gene63040:CDS |
| AACTCCTTCTTTGTCTGCTA+TGG | 0.395630 | 5.4:-44783279 | MS.gene63040:CDS |
| AGTTGGAATTTCACTTCAAA+AGG | 0.400364 | 5.4:-44782898 | MS.gene63040:CDS |
| TCTTCCCAAGGTCCATTAAC+AGG | 0.401821 | 5.4:+44782447 | None:intergenic |
| TCAAACGCTTGTTTGAAATC+AGG | 0.404416 | 5.4:+44782732 | None:intergenic |
| TCTTCCATTGTTGGTTTAGG+TGG | 0.408011 | 5.4:+44783419 | None:intergenic |
| CCCATTCTGAATAAACAGTT+TGG | 0.412390 | 5.4:+44783062 | None:intergenic |
| ATAAGAATGTCTATGTCCTT+AGG | 0.415605 | 5.4:+44783326 | None:intergenic |
| AGAATGACGCTGCATCGATT+CGG | 0.416495 | 5.4:-44782621 | MS.gene63040:CDS |
| ATTTAAAGCTTAACAATCTC+AGG | 0.423489 | 5.4:+44782396 | None:intergenic |
| GATACCTTGTTTGCGAAAAC+CGG | 0.424265 | 5.4:-44783356 | MS.gene63040:CDS |
| AAAGGGACGCGGCAAGTAAT+CGG | 0.425547 | 5.4:+44783572 | None:intergenic |
| TTGGTTTAGGTGGGATGTAA+TGG | 0.427468 | 5.4:+44783429 | None:intergenic |
| ATTCTCGAACGATCTGGTCT+TGG | 0.430456 | 5.4:-44783479 | MS.gene63040:CDS |
| CCAAACTGTTTATTCAGAAT+GGG | 0.431991 | 5.4:-44783062 | MS.gene63040:CDS |
| TTCATAGAACTACTACGTTT+AGG | 0.436422 | 5.4:-44783746 | MS.gene63040:CDS |
| CTTCCATTGTTGGTTTAGGT+GGG | 0.436694 | 5.4:+44783420 | None:intergenic |
| ACAATCTCAGGAATATGAAC+TGG | 0.440934 | 5.4:+44782408 | None:intergenic |
| AACACCTGTTAATGGACCTT+GGG | 0.444465 | 5.4:-44782451 | MS.gene63040:CDS |
| AAAAGATTGAGTATTTCAAA+AGG | 0.452047 | 5.4:+44783722 | None:intergenic |
| TTGAACTATATTGAATCAAA+AGG | 0.463579 | 5.4:-44782567 | MS.gene63040:CDS |
| AAACACCTGTTAATGGACCT+TGG | 0.472087 | 5.4:-44782452 | MS.gene63040:CDS |
| TTTGTATGTGATCAAATTGA+AGG | 0.474408 | 5.4:+44783693 | None:intergenic |
| ACCCGCGCTACATCCCATTC+CGG | 0.483459 | 5.4:+44783208 | None:intergenic |
| ATGAGAATTCTCGAACGATC+TGG | 0.483710 | 5.4:-44783485 | MS.gene63040:CDS |
| GCTTGTTATCTTCTCATCCA+TGG | 0.488002 | 5.4:-44783378 | MS.gene63040:CDS |
| AGTAATCGGCGGTTTGAAGC+AGG | 0.491235 | 5.4:+44783586 | None:intergenic |
| AAGAGTAATTGTTCTGAAGC+AGG | 0.493059 | 5.4:+44782810 | None:intergenic |
| ATCAAGAGCTTCAACCTCTC+CGG | 0.495473 | 5.4:-44783227 | MS.gene63040:CDS |
| GAGCTTCAACCTCTCCGGAA+TGG | 0.499688 | 5.4:-44783222 | MS.gene63040:CDS |
| TATGTCCTTAGGTTTGAGTC+CGG | 0.505098 | 5.4:+44783337 | None:intergenic |
| AAGATCGCGGGCTAAATCAA+TGG | 0.510668 | 5.4:+44783178 | None:intergenic |
| TCCGGAATGGGATGTAGCGC+GGG | 0.514072 | 5.4:-44783209 | MS.gene63040:CDS |
| AAAGATTGAGTATTTCAAAA+GGG | 0.517896 | 5.4:+44783723 | None:intergenic |
| CTTTAACATGAAGTATATTG+TGG | 0.524324 | 5.4:+44783637 | None:intergenic |
| AAGGATCTTATGGCTATAGC+AGG | 0.535171 | 5.4:-44782879 | MS.gene63040:CDS |
| CTCCGGAATGGGATGTAGCG+CGG | 0.535668 | 5.4:-44783210 | MS.gene63040:CDS |
| TTTCGCAAACAAGGTATCCA+TGG | 0.546419 | 5.4:+44783361 | None:intergenic |
| AAGAAGGAGTTGGAGAAAAG+AGG | 0.546500 | 5.4:+44783291 | None:intergenic |
| ATAGCAGACAAAGAAGGAGT+TGG | 0.559010 | 5.4:+44783281 | None:intergenic |
| CATCCCACCTAAACCAACAA+TGG | 0.566152 | 5.4:-44783423 | MS.gene63040:CDS |
| TCAAAAGGAAGGATGAAGAG+AGG | 0.574451 | 5.4:-44782552 | MS.gene63040:CDS |
| ATTACTCCAAACTATTACCA+AGG | 0.582645 | 5.4:-44783107 | MS.gene63040:CDS |
| GTTCCATGAAAGTAGCGAAA+GGG | 0.584347 | 5.4:+44783555 | None:intergenic |
| TTGTATGTGATCAAATTGAA+GGG | 0.587629 | 5.4:+44783694 | None:intergenic |
| TGAAAGTAGCGAAAGGGACG+CGG | 0.588590 | 5.4:+44783561 | None:intergenic |
| TTCGAAGAAGAAGACAGAGA+AGG | 0.591426 | 5.4:-44782924 | MS.gene63040:CDS |
| ACTATATTGAATCAAAAGGA+AGG | 0.597855 | 5.4:-44782563 | MS.gene63040:CDS |
| CTTCTGTAACTCATCGATCA+CGG | 0.605553 | 5.4:+44782680 | None:intergenic |
| ATCACCATAGCAGACAAAGA+AGG | 0.610156 | 5.4:+44783275 | None:intergenic |
| ATAAACCTGAAGAAGATCGC+GGG | 0.612944 | 5.4:+44783166 | None:intergenic |
| AACTGTTTATTCAGAATGGG+TGG | 0.613269 | 5.4:-44783059 | MS.gene63040:CDS |
| GGATGAGAAGATAACAAGCT+CGG | 0.613593 | 5.4:+44783382 | None:intergenic |
| TGTTCCATGAAAGTAGCGAA+AGG | 0.619184 | 5.4:+44783554 | None:intergenic |
| TTATGAGAAAACAAGAGCAA+AGG | 0.623278 | 5.4:+44783669 | None:intergenic |
| CTTCTTCTTCGAAAACGCAA+CGG | 0.633833 | 5.4:+44782934 | None:intergenic |
| GAAAACCGGACTCAAACCTA+AGG | 0.634039 | 5.4:-44783342 | MS.gene63040:CDS |
| TGAAGCAGGCATAATCGACT+AGG | 0.636758 | 5.4:+44783600 | None:intergenic |
| TCTGAAGCAGGTAGAACAAG+AGG | 0.638656 | 5.4:+44782822 | None:intergenic |
| CGATCAATGCAATCTTCCCA+AGG | 0.642968 | 5.4:+44782435 | None:intergenic |
| CGCTACATCCCATTCCGGAG+AGG | 0.648049 | 5.4:+44783213 | None:intergenic |
| CAAGTTGTTAGAACTCACAA+AGG | 0.665486 | 5.4:-44782975 | MS.gene63040:CDS |
| GCTATCTGCTGAACACGTCG+AGG | 0.667644 | 5.4:-44782649 | MS.gene63040:CDS |
| GATAAACCTGAAGAAGATCG+CGG | 0.670461 | 5.4:+44783165 | None:intergenic |
| TCGACTAGGTAGATTGCGCG+TGG | 0.676158 | 5.4:+44783614 | None:intergenic |
| GTTGTTAGAACTCACAAAGG+AGG | 0.692073 | 5.4:-44782972 | MS.gene63040:CDS |
| GGGATGTAATGGATTGCTGG+TGG | 0.704587 | 5.4:+44783440 | None:intergenic |
| TTAAATGCAATAGTGCTGTG+TGG | 0.711523 | 5.4:-44782494 | MS.gene63040:CDS |
| GGGACGCGGCAAGTAATCGG+CGG | 0.714953 | 5.4:+44783575 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCAAATATAACAACTAT+TGG | - | chr5.4:44783310-44783329 | MS.gene63040:CDS | 15.0% |
| !! | AAAAGATTGAGTATTTCAAA+AGG | + | chr5.4:44782437-44782456 | None:intergenic | 20.0% |
| !! | AAAGATTGAGTATTTCAAAA+GGG | + | chr5.4:44782436-44782455 | None:intergenic | 20.0% |
| !! | TTGAACTATATTGAATCAAA+AGG | - | chr5.4:44783589-44783608 | MS.gene63040:CDS | 20.0% |
| ! | ACTATATTGAATCAAAAGGA+AGG | - | chr5.4:44783593-44783612 | MS.gene63040:CDS | 25.0% |
| ! | CTTTAACATGAAGTATATTG+TGG | + | chr5.4:44782522-44782541 | None:intergenic | 25.0% |
| ! | TTGTATGTGATCAAATTGAA+GGG | + | chr5.4:44782465-44782484 | None:intergenic | 25.0% |
| ! | TTTGTATGTGATCAAATTGA+AGG | + | chr5.4:44782466-44782485 | None:intergenic | 25.0% |
| !! | TGTTTGAAATCAGGAATATA+AGG | + | chr5.4:44783418-44783437 | None:intergenic | 25.0% |
| ACACATCTTCTTCATCTTTA+TGG | - | chr5.4:44783560-44783579 | MS.gene63040:CDS | 30.0% | |
| ACCAAACTGTTTATTCAGAA+TGG | - | chr5.4:44783093-44783112 | MS.gene63040:CDS | 30.0% | |
| AGCATTAAAACACCTGTTAA+TGG | - | chr5.4:44783697-44783716 | MS.gene63040:CDS | 30.0% | |
| AGTTGGAATTTCACTTCAAA+AGG | - | chr5.4:44783258-44783277 | MS.gene63040:CDS | 30.0% | |
| ATAAGAATGTCTATGTCCTT+AGG | + | chr5.4:44782833-44782852 | None:intergenic | 30.0% | |
| ATTACTCCAAACTATTACCA+AGG | - | chr5.4:44783049-44783068 | MS.gene63040:CDS | 30.0% | |
| CCAAACTGTTTATTCAGAAT+GGG | - | chr5.4:44783094-44783113 | MS.gene63040:CDS | 30.0% | |
| TTATGAGAAAACAAGAGCAA+AGG | + | chr5.4:44782490-44782509 | None:intergenic | 30.0% | |
| TTCACTTCAAAAGGATCTTA+TGG | - | chr5.4:44783267-44783286 | MS.gene63040:CDS | 30.0% | |
| TTCATAGAACTACTACGTTT+AGG | - | chr5.4:44782410-44782429 | MS.gene63040:CDS | 30.0% | |
| ! | GGAATATAAGGCTTCATTTT+AGG | + | chr5.4:44783406-44783425 | None:intergenic | 30.0% |
| !! | ATTTGGAATTCAACGCTTTT+CGG | + | chr5.4:44782653-44782672 | None:intergenic | 30.0% |
| !! | TCTTTTCCTTGGTAATAGTT+TGG | + | chr5.4:44783058-44783077 | None:intergenic | 30.0% |
| AACTGTTTATTCAGAATGGG+TGG | - | chr5.4:44783097-44783116 | MS.gene63040:CDS | 35.0% | |
| ACAACAACTGCGTTTGAATT+CGG | + | chr5.4:44783016-44783035 | None:intergenic | 35.0% | |
| ACAATCTCAGGAATATGAAC+TGG | + | chr5.4:44783751-44783770 | None:intergenic | 35.0% | |
| ATCGTTCGAGAATTCTCATT+TGG | + | chr5.4:44782670-44782689 | None:intergenic | 35.0% | |
| CAAGTTGTTAGAACTCACAA+AGG | - | chr5.4:44783181-44783200 | MS.gene63040:CDS | 35.0% | |
| CCCATTCTGAATAAACAGTT+TGG | + | chr5.4:44783097-44783116 | None:intergenic | 35.0% | |
| TTAAATGCAATAGTGCTGTG+TGG | - | chr5.4:44783662-44783681 | MS.gene63040:CDS | 35.0% | |
| ! | AGAGTATGGCAAATAGCTTT+TGG | - | chr5.4:44783631-44783650 | MS.gene63040:CDS | 35.0% |
| ! | CATTTTTGTATTCATGCTGG+TGG | - | chr5.4:44783448-44783467 | MS.gene63040:CDS | 35.0% |
| ! | CTCTTTCTTTTCACACTCAT+TGG | - | chr5.4:44783364-44783383 | MS.gene63040:CDS | 35.0% |
| ! | GAGCATTTTTGTATTCATGC+TGG | - | chr5.4:44783445-44783464 | MS.gene63040:CDS | 35.0% |
| ! | TCAAACGCTTGTTTGAAATC+AGG | + | chr5.4:44783427-44783446 | None:intergenic | 35.0% |
| !! | AAGAGTAATTGTTCTGAAGC+AGG | + | chr5.4:44783349-44783368 | None:intergenic | 35.0% |
| !! | TTTGGAATTCAACGCTTTTC+GGG | + | chr5.4:44782652-44782671 | None:intergenic | 35.0% |
| AACTCCTTCTTTGTCTGCTA+TGG | - | chr5.4:44782877-44782896 | MS.gene63040:CDS | 40.0% | |
| AAGAAGGAGTTGGAGAAAAG+AGG | + | chr5.4:44782868-44782887 | None:intergenic | 40.0% | |
| AAGGATCTTATGGCTATAGC+AGG | - | chr5.4:44783277-44783296 | MS.gene63040:CDS | 40.0% | |
| ATAAACCTGAAGAAGATCGC+GGG | + | chr5.4:44782993-44783012 | None:intergenic | 40.0% | |
| ATAGCAGACAAAGAAGGAGT+TGG | + | chr5.4:44782878-44782897 | None:intergenic | 40.0% | |
| ATCACCATAGCAGACAAAGA+AGG | + | chr5.4:44782884-44782903 | None:intergenic | 40.0% | |
| ATGAGAATTCTCGAACGATC+TGG | - | chr5.4:44782671-44782690 | MS.gene63040:CDS | 40.0% | |
| CTTCTGTAACTCATCGATCA+CGG | + | chr5.4:44783479-44783498 | None:intergenic | 40.0% | |
| CTTCTTCTTCGAAAACGCAA+CGG | + | chr5.4:44783225-44783244 | None:intergenic | 40.0% | |
| GAAGACAGAGAAGGTAAAGT+TGG | - | chr5.4:44783241-44783260 | MS.gene63040:CDS | 40.0% | |
| GATAAACCTGAAGAAGATCG+CGG | + | chr5.4:44782994-44783013 | None:intergenic | 40.0% | |
| GGATGAGAAGATAACAAGCT+CGG | + | chr5.4:44782777-44782796 | None:intergenic | 40.0% | |
| GTTCCATGAAAGTAGCGAAA+GGG | + | chr5.4:44782604-44782623 | None:intergenic | 40.0% | |
| GTTGTTAGAACTCACAAAGG+AGG | - | chr5.4:44783184-44783203 | MS.gene63040:CDS | 40.0% | |
| TATGTCCTTAGGTTTGAGTC+CGG | + | chr5.4:44782822-44782841 | None:intergenic | 40.0% | |
| TCAAAAGGAAGGATGAAGAG+AGG | - | chr5.4:44783604-44783623 | MS.gene63040:CDS | 40.0% | |
| TGAAGAGAGGTGATAGAGTA+TGG | - | chr5.4:44783617-44783636 | MS.gene63040:CDS | 40.0% | |
| TGTTCCATGAAAGTAGCGAA+AGG | + | chr5.4:44782605-44782624 | None:intergenic | 40.0% | |
| TTCGAAGAAGAAGACAGAGA+AGG | - | chr5.4:44783232-44783251 | MS.gene63040:CDS | 40.0% | |
| TTGGTTTAGGTGGGATGTAA+TGG | + | chr5.4:44782730-44782749 | None:intergenic | 40.0% | |
| ! | AAACACCTGTTAATGGACCT+TGG | - | chr5.4:44783704-44783723 | MS.gene63040:CDS | 40.0% |
| ! | AACACCTGTTAATGGACCTT+GGG | - | chr5.4:44783705-44783724 | MS.gene63040:CDS | 40.0% |
| ! | GATACCTTGTTTGCGAAAAC+CGG | - | chr5.4:44782800-44782819 | MS.gene63040:CDS | 40.0% |
| ! | GCATTGCTCTTTCTTTTCCT+TGG | + | chr5.4:44783069-44783088 | None:intergenic | 40.0% |
| ! | TTTCGCAAACAAGGTATCCA+TGG | + | chr5.4:44782798-44782817 | None:intergenic | 40.0% |
| !! | CTTCCATTGTTGGTTTAGGT+GGG | + | chr5.4:44782739-44782758 | None:intergenic | 40.0% |
| !! | GCTTCTTCCATTGTTGGTTT+AGG | + | chr5.4:44782743-44782762 | None:intergenic | 40.0% |
| !! | GCTTGTTATCTTCTCATCCA+TGG | - | chr5.4:44782778-44782797 | MS.gene63040:CDS | 40.0% |
| !! | TCTTCCATTGTTGGTTTAGG+TGG | + | chr5.4:44782740-44782759 | None:intergenic | 40.0% |
| !! | TGGCAAATAGCTTTTGGAAG+TGG | - | chr5.4:44783637-44783656 | MS.gene63040:CDS | 40.0% |
| ATCAAGAGCTTCAACCTCTC+CGG | - | chr5.4:44782929-44782948 | MS.gene63040:CDS | 45.0% | |
| ATTCTCGAACGATCTGGTCT+TGG | - | chr5.4:44782677-44782696 | MS.gene63040:CDS | 45.0% | |
| CATCCCACCTAAACCAACAA+TGG | - | chr5.4:44782733-44782752 | MS.gene63040:CDS | 45.0% | |
| CGATCAATGCAATCTTCCCA+AGG | + | chr5.4:44783724-44783743 | None:intergenic | 45.0% | |
| CGTCTTGCTTCTTCCATTGT+TGG | + | chr5.4:44782749-44782768 | None:intergenic | 45.0% | |
| GAAAACCGGACTCAAACCTA+AGG | - | chr5.4:44782814-44782833 | MS.gene63040:CDS | 45.0% | |
| TCTTCCCAAGGTCCATTAAC+AGG | + | chr5.4:44783712-44783731 | None:intergenic | 45.0% | |
| TGAAGCAGGCATAATCGACT+AGG | + | chr5.4:44782559-44782578 | None:intergenic | 45.0% | |
| ! | AAGATCGCGGGCTAAATCAA+TGG | + | chr5.4:44782981-44783000 | None:intergenic | 45.0% |
| ! | TCTGAAGCAGGTAGAACAAG+AGG | + | chr5.4:44783337-44783356 | None:intergenic | 45.0% |
| !! | AGAATGACGCTGCATCGATT+CGG | - | chr5.4:44783535-44783554 | MS.gene63040:CDS | 45.0% |
| AAAGGGACGCGGCAAGTAAT+CGG | + | chr5.4:44782587-44782606 | None:intergenic | 50.0% | |
| AGCTTCAACCTCTCCGGAAT+GGG | - | chr5.4:44782935-44782954 | MS.gene63040:CDS | 50.0% | |
| TGAAAGTAGCGAAAGGGACG+CGG | + | chr5.4:44782598-44782617 | None:intergenic | 50.0% | |
| TTTAGCCCGCGATCTTCTTC+AGG | - | chr5.4:44782985-44783004 | MS.gene63040:CDS | 50.0% | |
| ! | AGTAATCGGCGGTTTGAAGC+AGG | + | chr5.4:44782573-44782592 | None:intergenic | 50.0% |
| ! | CGTCCCTTTCGCTACTTTCA+TGG | - | chr5.4:44782598-44782617 | MS.gene63040:CDS | 50.0% |
| ! | GAGTCCGGTTTTCGCAAACA+AGG | + | chr5.4:44782807-44782826 | None:intergenic | 50.0% |
| !! | GAATGACGCTGCATCGATTC+GGG | - | chr5.4:44783536-44783555 | MS.gene63040:CDS | 50.0% |
| !! | GGGATGTAATGGATTGCTGG+TGG | + | chr5.4:44782719-44782738 | None:intergenic | 50.0% |
| !! | GGTGGGATGTAATGGATTGC+TGG | + | chr5.4:44782722-44782741 | None:intergenic | 50.0% |
| !!! | CGCTTTTCGGGTTGTTCTTG+AGG | + | chr5.4:44782640-44782659 | None:intergenic | 50.0% |
| GAGCTTCAACCTCTCCGGAA+TGG | - | chr5.4:44782934-44782953 | MS.gene63040:CDS | 55.0% | |
| GCTATCTGCTGAACACGTCG+AGG | - | chr5.4:44783507-44783526 | MS.gene63040:CDS | 55.0% | |
| TCGACTAGGTAGATTGCGCG+TGG | + | chr5.4:44782545-44782564 | None:intergenic | 55.0% | |
| ACCCGCGCTACATCCCATTC+CGG | + | chr5.4:44782951-44782970 | None:intergenic | 60.0% | |
| CGCTACATCCCATTCCGGAG+AGG | + | chr5.4:44782946-44782965 | None:intergenic | 60.0% | |
| CTCCGGAATGGGATGTAGCG+CGG | - | chr5.4:44782946-44782965 | MS.gene63040:CDS | 60.0% | |
| TCCGGAATGGGATGTAGCGC+GGG | - | chr5.4:44782947-44782966 | MS.gene63040:CDS | 60.0% | |
| GGGACGCGGCAAGTAATCGG+CGG | + | chr5.4:44782584-44782603 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 44782398 | 44783780 | 44782398 | ID=MS.gene63040 |
| chr5.4 | mRNA | 44782398 | 44783780 | 44782398 | ID=MS.gene63040.t1;Parent=MS.gene63040 |
| chr5.4 | exon | 44782398 | 44783780 | 44782398 | ID=MS.gene63040.t1.exon1;Parent=MS.gene63040.t1 |
| chr5.4 | CDS | 44782398 | 44783780 | 44782398 | ID=cds.MS.gene63040.t1;Parent=MS.gene63040.t1 |
| Gene Sequence |
| Protein sequence |
