Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69083.t1 | XP_003603663.1 | 94.2 | 104 | 6 | 0 | 1 | 104 | 1 | 104 | 3.40E-40 | 174.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69083.t1 | P11892 | 85.4 | 89 | 12 | 1 | 1 | 88 | 1 | 89 | 9.7e-36 | 150.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69083.t1 | B7FHD4 | 94.2 | 104 | 6 | 0 | 1 | 104 | 1 | 104 | 2.5e-40 | 174.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene69083 | 0.808693 | 2.73E-50 | -1.69E-46 |
| MS.gene049346 | MS.gene69083 | 0.800273 | 1.56E-48 | -1.69E-46 |
| MS.gene049389 | MS.gene69083 | 0.830954 | 2.20E-55 | -1.69E-46 |
| MS.gene049491 | MS.gene69083 | 0.844145 | 9.06E-59 | -1.69E-46 |
| MS.gene049661 | MS.gene69083 | 0.801806 | 7.57E-49 | -1.69E-46 |
| MS.gene049662 | MS.gene69083 | 0.808302 | 3.31E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene69083 | 0.894678 | 1.97E-75 | -1.69E-46 |
| MS.gene049910 | MS.gene69083 | 0.80405 | 2.60E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene69083 | 0.845826 | 3.19E-59 | -1.69E-46 |
| MS.gene050099 | MS.gene69083 | 0.856519 | 3.05E-62 | -1.69E-46 |
| MS.gene050128 | MS.gene69083 | 0.880933 | 3.65E-70 | -1.69E-46 |
| MS.gene050301 | MS.gene69083 | 0.875524 | 2.88E-68 | -1.69E-46 |
| MS.gene050397 | MS.gene69083 | 0.838562 | 2.67E-57 | -1.69E-46 |
| MS.gene050404 | MS.gene69083 | 0.84366 | 1.22E-58 | -1.69E-46 |
| MS.gene050406 | MS.gene69083 | 0.847385 | 1.20E-59 | -1.69E-46 |
| MS.gene050432 | MS.gene69083 | 0.820634 | 6.18E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene69083 | 0.826715 | 2.33E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene69083 | 0.846092 | 2.70E-59 | -1.69E-46 |
| MS.gene05053 | MS.gene69083 | 0.803035 | 4.22E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene69083 | 0.840349 | 9.17E-58 | -1.69E-46 |
| MS.gene050770 | MS.gene69083 | 0.842509 | 2.48E-58 | -1.69E-46 |
| MS.gene05101 | MS.gene69083 | 0.86424 | 1.40E-64 | -1.69E-46 |
| MS.gene051471 | MS.gene69083 | 0.873022 | 2.03E-67 | -1.69E-46 |
| MS.gene051662 | MS.gene69083 | 0.840927 | 6.48E-58 | -1.69E-46 |
| MS.gene051663 | MS.gene69083 | 0.836371 | 9.73E-57 | -1.69E-46 |
| MS.gene051860 | MS.gene69083 | 0.805373 | 1.38E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene69083 | 0.806731 | 7.13E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene69083 | 0.882194 | 1.28E-70 | -1.69E-46 |
| MS.gene052178 | MS.gene69083 | 0.88845 | 5.84E-73 | -1.69E-46 |
| MS.gene052188 | MS.gene69083 | 0.846326 | 2.33E-59 | -1.69E-46 |
| MS.gene052290 | MS.gene69083 | 0.847269 | 1.29E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene69083 | 0.864138 | 1.51E-64 | -1.69E-46 |
| MS.gene052326 | MS.gene69083 | 0.845655 | 3.55E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene69083 | 0.868999 | 4.30E-66 | -1.69E-46 |
| MS.gene053073 | MS.gene69083 | 0.853029 | 3.13E-61 | -1.69E-46 |
| MS.gene053103 | MS.gene69083 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene05316 | MS.gene69083 | 0.816497 | 5.36E-52 | -1.69E-46 |
| MS.gene053181 | MS.gene69083 | 0.812722 | 3.67E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene69083 | 0.847311 | 1.25E-59 | -1.69E-46 |
| MS.gene053557 | MS.gene69083 | 0.881274 | 2.75E-70 | -1.69E-46 |
| MS.gene053559 | MS.gene69083 | 0.806801 | 6.90E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene69083 | 0.87161 | 6.00E-67 | -1.69E-46 |
| MS.gene053907 | MS.gene69083 | 0.808679 | 2.75E-50 | -1.69E-46 |
| MS.gene053928 | MS.gene69083 | 0.810207 | 1.29E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene69083 | 0.837206 | 5.96E-57 | -1.69E-46 |
| MS.gene054376 | MS.gene69083 | 0.846015 | 2.83E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene69083 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene05455 | MS.gene69083 | 0.833957 | 3.96E-56 | -1.69E-46 |
| MS.gene054618 | MS.gene69083 | 0.814382 | 1.58E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene69083 | 0.80387 | 2.84E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene69083 | 0.806049 | 9.93E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene69083 | 0.807239 | 5.57E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene69083 | 0.834516 | 2.87E-56 | -1.69E-46 |
| MS.gene055133 | MS.gene69083 | 0.823724 | 1.19E-53 | -1.69E-46 |
| MS.gene055254 | MS.gene69083 | 0.849762 | 2.63E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene69083 | 0.882254 | 1.22E-70 | -1.69E-46 |
| MS.gene055390 | MS.gene69083 | 0.839159 | 1.87E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene69083 | 0.892128 | 2.11E-74 | -1.69E-46 |
| MS.gene055428 | MS.gene69083 | 0.854555 | 1.14E-61 | -1.69E-46 |
| MS.gene055604 | MS.gene69083 | 0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene055712 | MS.gene69083 | 0.832165 | 1.10E-55 | -1.69E-46 |
| MS.gene055896 | MS.gene69083 | 0.809424 | 1.91E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene69083 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene69083 | 0.823156 | 1.61E-53 | -1.69E-46 |
| MS.gene056314 | MS.gene69083 | 0.849741 | 2.66E-60 | -1.69E-46 |
| MS.gene056407 | MS.gene69083 | 0.849051 | 4.14E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene69083 | 0.828943 | 6.79E-55 | -1.69E-46 |
| MS.gene056609 | MS.gene69083 | 0.847117 | 1.42E-59 | -1.69E-46 |
| MS.gene056612 | MS.gene69083 | 0.86448 | 1.18E-64 | -1.69E-46 |
| MS.gene056719 | MS.gene69083 | 0.809422 | 1.91E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene69083 | 0.854476 | 1.20E-61 | -1.69E-46 |
| MS.gene057123 | MS.gene69083 | 0.819525 | 1.11E-52 | -1.69E-46 |
| MS.gene057295 | MS.gene69083 | 0.838503 | 2.77E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene69083 | 0.806712 | 7.20E-50 | -1.69E-46 |
| MS.gene057439 | MS.gene69083 | 0.876927 | 9.45E-69 | -1.69E-46 |
| MS.gene057448 | MS.gene69083 | 0.843524 | 1.33E-58 | -1.69E-46 |
| MS.gene057449 | MS.gene69083 | 0.833785 | 4.37E-56 | -1.69E-46 |
| MS.gene057450 | MS.gene69083 | 0.863475 | 2.43E-64 | -1.69E-46 |
| MS.gene057754 | MS.gene69083 | 0.809579 | 1.76E-50 | -1.69E-46 |
| MS.gene057794 | MS.gene69083 | 0.815473 | 9.08E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene69083 | 0.832163 | 1.11E-55 | -1.69E-46 |
| MS.gene057927 | MS.gene69083 | 0.808792 | 2.60E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene69083 | 0.841599 | 4.31E-58 | -1.69E-46 |
| MS.gene058082 | MS.gene69083 | 0.849346 | 3.43E-60 | -1.69E-46 |
| MS.gene05811 | MS.gene69083 | 0.867834 | 1.02E-65 | -1.69E-46 |
| MS.gene058233 | MS.gene69083 | 0.804574 | 2.02E-49 | -1.69E-46 |
| MS.gene058276 | MS.gene69083 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene69083 | 0.86845 | 6.47E-66 | -1.69E-46 |
| MS.gene058365 | MS.gene69083 | 0.826179 | 3.13E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene69083 | 0.852169 | 5.51E-61 | -1.69E-46 |
| MS.gene058787 | MS.gene69083 | 0.814318 | 1.64E-51 | -1.69E-46 |
| MS.gene058845 | MS.gene69083 | 0.843906 | 1.05E-58 | -1.69E-46 |
| MS.gene058928 | MS.gene69083 | 0.800115 | 1.68E-48 | -1.69E-46 |
| MS.gene05907 | MS.gene69083 | 0.858879 | 6.08E-63 | -1.69E-46 |
| MS.gene059228 | MS.gene69083 | 0.848578 | 5.61E-60 | -1.69E-46 |
| MS.gene059294 | MS.gene69083 | 0.819256 | 1.28E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene69083 | 0.87242 | 3.23E-67 | -1.69E-46 |
| MS.gene059518 | MS.gene69083 | 0.876024 | 1.94E-68 | -1.69E-46 |
| MS.gene059597 | MS.gene69083 | 0.869261 | 3.53E-66 | -1.69E-46 |
| MS.gene05959 | MS.gene69083 | 0.828076 | 1.10E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene69083 | 0.863489 | 2.40E-64 | -1.69E-46 |
| MS.gene059688 | MS.gene69083 | 0.845439 | 4.06E-59 | -1.69E-46 |
| MS.gene059692 | MS.gene69083 | 0.881462 | 2.35E-70 | -1.69E-46 |
| MS.gene059695 | MS.gene69083 | 0.874312 | 7.45E-68 | -1.69E-46 |
| MS.gene059708 | MS.gene69083 | 0.822477 | 2.32E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene69083 | 0.854768 | 9.88E-62 | -1.69E-46 |
| MS.gene059894 | MS.gene69083 | 0.900917 | 4.53E-78 | -1.69E-46 |
| MS.gene059899 | MS.gene69083 | 0.833783 | 4.37E-56 | -1.69E-46 |
| MS.gene059960 | MS.gene69083 | 0.802961 | 4.38E-49 | -1.69E-46 |
| MS.gene059974 | MS.gene69083 | 0.841799 | 3.82E-58 | -1.69E-46 |
| MS.gene060005 | MS.gene69083 | 0.852122 | 5.68E-61 | -1.69E-46 |
| MS.gene06037 | MS.gene69083 | 0.871242 | 7.93E-67 | -1.69E-46 |
| MS.gene060407 | MS.gene69083 | 0.802861 | 4.59E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene69083 | 0.840866 | 6.72E-58 | -1.69E-46 |
| MS.gene060495 | MS.gene69083 | 0.82857 | 8.36E-55 | -1.69E-46 |
| MS.gene060496 | MS.gene69083 | 0.80856 | 2.92E-50 | -1.69E-46 |
| MS.gene06090 | MS.gene69083 | 0.801286 | 9.67E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene69083 | 0.893508 | 5.89E-75 | -1.69E-46 |
| MS.gene061051 | MS.gene69083 | -0.860999 | 1.40E-63 | -1.69E-46 |
| MS.gene061112 | MS.gene69083 | 0.849874 | 2.44E-60 | -1.69E-46 |
| MS.gene061113 | MS.gene69083 | 0.807268 | 5.49E-50 | -1.69E-46 |
| MS.gene061265 | MS.gene69083 | 0.843925 | 1.04E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69083.t1 | MTR_3g110640 | 97.115 | 104 | 3 | 0 | 1 | 104 | 1 | 104 | 1.85e-69 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69083.t1 | AT5G17870 | 49.474 | 95 | 45 | 2 | 3 | 94 | 2 | 96 | 7.89e-23 | 85.1 |
| MS.gene69083.t1 | AT5G17870 | 49.474 | 95 | 45 | 2 | 3 | 94 | 25 | 119 | 1.89e-22 | 85.1 |
Find 27 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTCTTTCTCAATGAATT+TGG | 0.155637 | 3.4:-95861527 | None:intergenic |
| TCGTCGGTGTCAAGCATATT+CGG | 0.370597 | 3.4:+95861485 | MS.gene69083:CDS |
| TCATTGAGAAAGAAAGCAAT+TGG | 0.425202 | 3.4:+95861533 | MS.gene69083:CDS |
| GAAATGACAATTGTAAAATC+AGG | 0.433333 | 3.4:-95861716 | None:intergenic |
| CATCACTGTCGGATAGGAAT+CGG | 0.473566 | 3.4:+95861657 | MS.gene69083:CDS |
| TGTCAAGCATATTCGGATGC+GGG | 0.490823 | 3.4:+95861492 | MS.gene69083:CDS |
| AAGGTACCAAATCAAGTAGA+AGG | 0.494390 | 3.4:-95861785 | None:intergenic |
| AGAAAGCAATTGGAATTGAA+AGG | 0.494556 | 3.4:+95861543 | MS.gene69083:CDS |
| GTAGAAGGAGTAGGAGGCGG+AGG | 0.503700 | 3.4:-95861770 | None:intergenic |
| TGTGCAGTTGCTTTCTTCTG+TGG | 0.507932 | 3.4:-95861608 | None:intergenic |
| GTGTCAAGCATATTCGGATG+CGG | 0.540271 | 3.4:+95861491 | MS.gene69083:CDS |
| GGCAAAGGAGCATACTCTGT+TGG | 0.555146 | 3.4:-95861683 | None:intergenic |
| TCAGGAGGCAGTGGTGGCAA+AGG | 0.562798 | 3.4:-95861698 | None:intergenic |
| CCAAATCAAGTAGAAGGAGT+AGG | 0.568811 | 3.4:-95861779 | None:intergenic |
| ATTGTAAAATCAGGAGGCAG+TGG | 0.573977 | 3.4:-95861707 | None:intergenic |
| CAAGTAGAAGGAGTAGGAGG+CGG | 0.578017 | 3.4:-95861773 | None:intergenic |
| TGATGCATCATCAACGGCCA+CGG | 0.584871 | 3.4:+95861742 | MS.gene69083:CDS |
| TTCTGCTGATGCATCATCAA+CGG | 0.592398 | 3.4:+95861736 | MS.gene69083:CDS |
| GTCAAGCATATTCGGATGCG+GGG | 0.596492 | 3.4:+95861493 | MS.gene69083:CDS |
| GTAAAATCAGGAGGCAGTGG+TGG | 0.601246 | 3.4:-95861704 | None:intergenic |
| AATCAAGTAGAAGGAGTAGG+AGG | 0.618321 | 3.4:-95861776 | None:intergenic |
| ATGACAATTGTAAAATCAGG+AGG | 0.620980 | 3.4:-95861713 | None:intergenic |
| GAAAGGAGAAATGTATGTGG+AGG | 0.621499 | 3.4:+95861560 | MS.gene69083:CDS |
| ATTGAAAGGAGAAATGTATG+TGG | 0.642127 | 3.4:+95861557 | MS.gene69083:CDS |
| CTGCACATCACATGAAGACG+AGG | 0.645185 | 3.4:+95861624 | MS.gene69083:CDS |
| AGGAGGCGGAGGAACAACCG+TGG | 0.742635 | 3.4:-95861759 | None:intergenic |
| TGTTGATAGAGTGTTCATCG+AGG | 0.792218 | 3.4:+95861585 | MS.gene69083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAATGACAATTGTAAAATC+AGG | - | chr3.4:95861719-95861738 | None:intergenic | 25.0% |
| AGAAAGCAATTGGAATTGAA+AGG | + | chr3.4:95861543-95861562 | MS.gene69083:CDS | 30.0% | |
| ATGACAATTGTAAAATCAGG+AGG | - | chr3.4:95861716-95861735 | None:intergenic | 30.0% | |
| ATTGAAAGGAGAAATGTATG+TGG | + | chr3.4:95861557-95861576 | MS.gene69083:CDS | 30.0% | |
| GCTTTCTTTCTCAATGAATT+TGG | - | chr3.4:95861530-95861549 | None:intergenic | 30.0% | |
| TCATTGAGAAAGAAAGCAAT+TGG | + | chr3.4:95861533-95861552 | MS.gene69083:CDS | 30.0% | |
| AAAAATCATCACTGTCGGAT+AGG | + | chr3.4:95861651-95861670 | MS.gene69083:CDS | 35.0% | |
| !!! | ATCCGACAGTGATGATTTTT+TGG | - | chr3.4:95861651-95861670 | None:intergenic | 35.0% |
| !!! | TCCGACAGTGATGATTTTTT+GGG | - | chr3.4:95861650-95861669 | None:intergenic | 35.0% |
| ATTGTAAAATCAGGAGGCAG+TGG | - | chr3.4:95861710-95861729 | None:intergenic | 40.0% | |
| GAAAGGAGAAATGTATGTGG+AGG | + | chr3.4:95861560-95861579 | MS.gene69083:CDS | 40.0% | |
| GCCCAAAAAATCATCACTGT+CGG | + | chr3.4:95861646-95861665 | MS.gene69083:CDS | 40.0% | |
| TTCTGCTGATGCATCATCAA+CGG | + | chr3.4:95861736-95861755 | MS.gene69083:CDS | 40.0% | |
| ! | TGTTGATAGAGTGTTCATCG+AGG | + | chr3.4:95861585-95861604 | MS.gene69083:CDS | 40.0% |
| CATCACTGTCGGATAGGAAT+CGG | + | chr3.4:95861657-95861676 | MS.gene69083:CDS | 45.0% | |
| GTGTCAAGCATATTCGGATG+CGG | + | chr3.4:95861491-95861510 | MS.gene69083:CDS | 45.0% | |
| TGTCAAGCATATTCGGATGC+GGG | + | chr3.4:95861492-95861511 | MS.gene69083:CDS | 45.0% | |
| ! | TGTGCAGTTGCTTTCTTCTG+TGG | - | chr3.4:95861611-95861630 | None:intergenic | 45.0% |
| !! | TCGTCGGTGTCAAGCATATT+CGG | + | chr3.4:95861485-95861504 | MS.gene69083:CDS | 45.0% |
| CAAGTAGAAGGAGTAGGAGG+CGG | - | chr3.4:95861776-95861795 | None:intergenic | 50.0% | |
| CTGCACATCACATGAAGACG+AGG | + | chr3.4:95861624-95861643 | MS.gene69083:CDS | 50.0% | |
| GTAAAATCAGGAGGCAGTGG+TGG | - | chr3.4:95861707-95861726 | None:intergenic | 50.0% | |
| GTCAAGCATATTCGGATGCG+GGG | + | chr3.4:95861493-95861512 | MS.gene69083:CDS | 50.0% | |
| TGATGCATCATCAACGGCCA+CGG | + | chr3.4:95861742-95861761 | MS.gene69083:CDS | 50.0% | |
| ! | GGCAAAGGAGCATACTCTGT+TGG | - | chr3.4:95861686-95861705 | None:intergenic | 50.0% |
| GTAGAAGGAGTAGGAGGCGG+AGG | - | chr3.4:95861773-95861792 | None:intergenic | 60.0% | |
| TCAGGAGGCAGTGGTGGCAA+AGG | - | chr3.4:95861701-95861720 | None:intergenic | 60.0% | |
| AGGAGGCGGAGGAACAACCG+TGG | - | chr3.4:95861762-95861781 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 95861482 | 95861796 | 95861482 | ID=MS.gene69083 |
| chr3.4 | mRNA | 95861482 | 95861796 | 95861482 | ID=MS.gene69083.t1;Parent=MS.gene69083 |
| chr3.4 | exon | 95861482 | 95861796 | 95861482 | ID=MS.gene69083.t1.exon1;Parent=MS.gene69083.t1 |
| chr3.4 | CDS | 95861482 | 95861796 | 95861482 | ID=cds.MS.gene69083.t1;Parent=MS.gene69083.t1 |
| Gene Sequence |
| Protein sequence |
