Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79677.t1 | XP_003601746.1 | 92.6 | 363 | 22 | 2 | 1 | 363 | 1 | 358 | 6.70E-184 | 653.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79677.t1 | Q9SAC0 | 67.2 | 348 | 99 | 2 | 16 | 363 | 35 | 367 | 2.2e-135 | 483.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79677.t1 | B7FL73 | 92.6 | 363 | 22 | 2 | 1 | 363 | 1 | 358 | 4.8e-184 | 653.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene79677 | 0.801183 | 1.02E-48 | -1.69E-46 |
| MS.gene049170 | MS.gene79677 | 0.805673 | 1.19E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene79677 | 0.82008 | 8.28E-53 | -1.69E-46 |
| MS.gene049351 | MS.gene79677 | 0.832846 | 7.49E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene79677 | 0.8112 | 7.88E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene79677 | 0.809017 | 2.33E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene79677 | 0.813837 | 2.09E-51 | -1.69E-46 |
| MS.gene050099 | MS.gene79677 | 0.820097 | 8.21E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene79677 | 0.852135 | 5.63E-61 | -1.69E-46 |
| MS.gene050397 | MS.gene79677 | 0.838923 | 2.15E-57 | -1.69E-46 |
| MS.gene050400 | MS.gene79677 | 0.814083 | 1.85E-51 | -1.69E-46 |
| MS.gene050404 | MS.gene79677 | 0.820951 | 5.23E-53 | -1.69E-46 |
| MS.gene050406 | MS.gene79677 | 0.827554 | 1.47E-54 | -1.69E-46 |
| MS.gene050426 | MS.gene79677 | -0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene050464 | MS.gene79677 | 0.824189 | 9.23E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene79677 | 0.841149 | 5.66E-58 | -1.69E-46 |
| MS.gene050650 | MS.gene79677 | 0.804779 | 1.83E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene79677 | 0.862345 | 5.41E-64 | -1.69E-46 |
| MS.gene050812 | MS.gene79677 | 0.854936 | 8.83E-62 | -1.69E-46 |
| MS.gene05101 | MS.gene79677 | 0.837649 | 4.59E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene79677 | 0.845015 | 5.29E-59 | -1.69E-46 |
| MS.gene051171 | MS.gene79677 | 0.869032 | 4.19E-66 | -1.69E-46 |
| MS.gene051242 | MS.gene79677 | 0.836084 | 1.15E-56 | -1.69E-46 |
| MS.gene051244 | MS.gene79677 | 0.831294 | 1.81E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene79677 | 0.809841 | 1.55E-50 | -1.69E-46 |
| MS.gene051525 | MS.gene79677 | 0.842993 | 1.84E-58 | -1.69E-46 |
| MS.gene051582 | MS.gene79677 | 0.860634 | 1.80E-63 | -1.69E-46 |
| MS.gene052011 | MS.gene79677 | -0.844107 | 9.28E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene79677 | 0.864647 | 1.05E-64 | -1.69E-46 |
| MS.gene052188 | MS.gene79677 | 0.869876 | 2.23E-66 | -1.69E-46 |
| MS.gene052253 | MS.gene79677 | 0.866785 | 2.21E-65 | -1.69E-46 |
| MS.gene052310 | MS.gene79677 | 0.870429 | 1.47E-66 | -1.69E-46 |
| MS.gene052322 | MS.gene79677 | 0.840719 | 7.34E-58 | -1.69E-46 |
| MS.gene052340 | MS.gene79677 | 0.864328 | 1.32E-64 | -1.69E-46 |
| MS.gene052481 | MS.gene79677 | 0.850494 | 1.64E-60 | -1.69E-46 |
| MS.gene052484 | MS.gene79677 | 0.814464 | 1.52E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene79677 | 0.843193 | 1.63E-58 | -1.69E-46 |
| MS.gene052982 | MS.gene79677 | 0.810075 | 1.38E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene79677 | 0.863041 | 3.30E-64 | -1.69E-46 |
| MS.gene053330 | MS.gene79677 | 0.833866 | 4.17E-56 | -1.69E-46 |
| MS.gene053557 | MS.gene79677 | 0.81221 | 4.75E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene79677 | 0.832803 | 7.68E-56 | -1.69E-46 |
| MS.gene053928 | MS.gene79677 | 0.822172 | 2.73E-53 | -1.69E-46 |
| MS.gene053971 | MS.gene79677 | 0.81189 | 5.58E-51 | -1.69E-46 |
| MS.gene054123 | MS.gene79677 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene79677 | 0.816256 | 6.07E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene79677 | 0.80847 | 3.05E-50 | -1.69E-46 |
| MS.gene054719 | MS.gene79677 | 0.818227 | 2.19E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene79677 | 0.855613 | 5.61E-62 | -1.69E-46 |
| MS.gene055277 | MS.gene79677 | 0.826835 | 2.18E-54 | -1.69E-46 |
| MS.gene055370 | MS.gene79677 | 0.814978 | 1.17E-51 | -1.69E-46 |
| MS.gene055412 | MS.gene79677 | 0.802291 | 6.02E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene79677 | 0.833243 | 5.97E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene79677 | 0.837819 | 4.15E-57 | -1.69E-46 |
| MS.gene055539 | MS.gene79677 | 0.814791 | 1.29E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene79677 | 0.848522 | 5.81E-60 | -1.69E-46 |
| MS.gene055604 | MS.gene79677 | 0.807114 | 5.92E-50 | -1.69E-46 |
| MS.gene055971 | MS.gene79677 | 0.821162 | 4.67E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene79677 | 0.83259 | 8.67E-56 | -1.69E-46 |
| MS.gene056407 | MS.gene79677 | 0.813902 | 2.02E-51 | -1.69E-46 |
| MS.gene056497 | MS.gene79677 | 0.81046 | 1.14E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene79677 | 0.818105 | 2.33E-52 | -1.69E-46 |
| MS.gene056633 | MS.gene79677 | 0.821254 | 4.45E-53 | -1.69E-46 |
| MS.gene056659 | MS.gene79677 | 0.835346 | 1.77E-56 | -1.69E-46 |
| MS.gene056664 | MS.gene79677 | 0.815733 | 7.95E-52 | -1.69E-46 |
| MS.gene056796 | MS.gene79677 | 0.874182 | 8.25E-68 | -1.69E-46 |
| MS.gene056897 | MS.gene79677 | 0.804217 | 2.40E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene79677 | -0.809451 | 1.88E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene79677 | 0.828289 | 9.76E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene79677 | 0.824741 | 6.85E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene79677 | 0.861074 | 1.32E-63 | -1.69E-46 |
| MS.gene057196 | MS.gene79677 | 0.827731 | 1.33E-54 | -1.69E-46 |
| MS.gene05733 | MS.gene79677 | 0.815831 | 7.56E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene79677 | 0.844728 | 6.31E-59 | -1.69E-46 |
| MS.gene057823 | MS.gene79677 | 0.839448 | 1.58E-57 | -1.69E-46 |
| MS.gene057825 | MS.gene79677 | 0.80524 | 1.47E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene79677 | 0.872736 | 2.53E-67 | -1.69E-46 |
| MS.gene058007 | MS.gene79677 | 0.805122 | 1.55E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene79677 | 0.836322 | 1.00E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene79677 | 0.855101 | 7.91E-62 | -1.69E-46 |
| MS.gene058142 | MS.gene79677 | 0.846403 | 2.22E-59 | -1.69E-46 |
| MS.gene058233 | MS.gene79677 | 0.865831 | 4.44E-65 | -1.69E-46 |
| MS.gene058276 | MS.gene79677 | 0.865402 | 6.06E-65 | -1.69E-46 |
| MS.gene058293 | MS.gene79677 | 0.838761 | 2.37E-57 | -1.69E-46 |
| MS.gene058365 | MS.gene79677 | 0.838205 | 3.30E-57 | -1.69E-46 |
| MS.gene058444 | MS.gene79677 | 0.839952 | 1.16E-57 | -1.69E-46 |
| MS.gene058660 | MS.gene79677 | 0.844789 | 6.08E-59 | -1.69E-46 |
| MS.gene058700 | MS.gene79677 | 0.815451 | 9.18E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene79677 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene79677 | 0.846659 | 1.89E-59 | -1.69E-46 |
| MS.gene058787 | MS.gene79677 | 0.823617 | 1.26E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene79677 | 0.847116 | 1.42E-59 | -1.69E-46 |
| MS.gene058993 | MS.gene79677 | 0.823362 | 1.44E-53 | -1.69E-46 |
| MS.gene059011 | MS.gene79677 | 0.804665 | 1.94E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene79677 | 0.836158 | 1.10E-56 | -1.69E-46 |
| MS.gene059141 | MS.gene79677 | 0.813504 | 2.48E-51 | -1.69E-46 |
| MS.gene059294 | MS.gene79677 | 0.80262 | 5.14E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene79677 | 0.831268 | 1.84E-55 | -1.69E-46 |
| MS.gene059518 | MS.gene79677 | 0.830269 | 3.23E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene79677 | 0.827007 | 1.98E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene79677 | 0.855146 | 7.67E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene79677 | 0.857775 | 1.30E-62 | -1.69E-46 |
| MS.gene059692 | MS.gene79677 | 0.87711 | 8.17E-69 | -1.69E-46 |
| MS.gene059695 | MS.gene79677 | 0.878523 | 2.62E-69 | -1.69E-46 |
| MS.gene05984 | MS.gene79677 | 0.861998 | 6.92E-64 | -1.69E-46 |
| MS.gene059894 | MS.gene79677 | 0.832814 | 7.62E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene79677 | 0.820976 | 5.16E-53 | -1.69E-46 |
| MS.gene060407 | MS.gene79677 | 0.865774 | 4.63E-65 | -1.69E-46 |
| MS.gene061049 | MS.gene79677 | 0.867348 | 1.46E-65 | -1.69E-46 |
| MS.gene061154 | MS.gene79677 | 0.83948 | 1.55E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79677.t1 | MTR_3g084950 | 94.215 | 363 | 16 | 2 | 1 | 363 | 1 | 358 | 0.0 | 658 |
| MS.gene79677.t1 | MTR_8g097190 | 71.594 | 345 | 86 | 3 | 22 | 363 | 29 | 364 | 0.0 | 526 |
| MS.gene79677.t1 | MTR_8g097190 | 63.158 | 247 | 79 | 3 | 22 | 265 | 29 | 266 | 7.10e-111 | 324 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79677.t1 | AT1G10830 | 76.897 | 290 | 67 | 0 | 74 | 363 | 78 | 367 | 1.13e-177 | 498 |
| MS.gene79677.t1 | AT1G10830 | 73.936 | 188 | 49 | 0 | 74 | 261 | 78 | 265 | 6.57e-103 | 305 |
| MS.gene79677.t1 | AT1G10830 | 74.545 | 110 | 28 | 0 | 74 | 183 | 78 | 187 | 1.13e-52 | 174 |
Find 78 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATTTCTAAAAGATTAA+AGG | 0.126652 | 3.1:+64866404 | None:intergenic |
| GATTTATTTCGCCGCAATTT+TGG | 0.154342 | 3.1:-64867465 | MS.gene79677:CDS |
| TTAATTTCACATCATCTATT+TGG | 0.187467 | 3.1:-64865858 | MS.gene79677:CDS |
| CATTACCCATTCTTCAATAA+AGG | 0.211962 | 3.1:-64867558 | MS.gene79677:CDS |
| AAACCGCAAATATGAGAATT+AGG | 0.277606 | 3.1:+64866803 | None:intergenic |
| GGTGTCTTGCTCATACAATT+TGG | 0.284942 | 3.1:-64865917 | MS.gene79677:CDS |
| TATGAGGAGCGTTGAGAAGT+AGG | 0.327710 | 3.1:+64867644 | None:intergenic |
| AATGCAGTAATTACTAAGTA+TGG | 0.338106 | 3.1:+64865687 | None:intergenic |
| GTGGCTGTTGCAGCTTCAAT+TGG | 0.349115 | 3.1:-64865882 | MS.gene79677:CDS |
| AGATGGTTGGTCAGGTAATC+TGG | 0.354286 | 3.1:-64865938 | MS.gene79677:CDS |
| TGCTGTTCAGATGGTTGGTC+AGG | 0.355164 | 3.1:-64865946 | MS.gene79677:intron |
| ATTACCCATTCTTCAATAAA+GGG | 0.356103 | 3.1:-64867557 | MS.gene79677:CDS |
| GAGCGTTGAGAAGTAGGTTT+TGG | 0.361636 | 3.1:+64867650 | None:intergenic |
| CACATCATCTATTTGGTGCT+TGG | 0.366412 | 3.1:-64865851 | MS.gene79677:CDS |
| AAAATACATCTATATGAAAC+TGG | 0.374022 | 3.1:-64866366 | MS.gene79677:CDS |
| TCTCTCACAGGTTCAACTTC+AGG | 0.382575 | 3.1:-64867583 | MS.gene79677:CDS |
| CGGAACAGAAAATAACTTCA+TGG | 0.384156 | 3.1:-64867487 | MS.gene79677:CDS |
| TTTATTAATCACAGATATGA+TGG | 0.389024 | 3.1:-64866519 | MS.gene79677:CDS |
| CCATCAAGGATTGCTGCAAA+AGG | 0.390457 | 3.1:+64865756 | None:intergenic |
| ATTGATAACTCAACTGGCTT+TGG | 0.394276 | 3.1:-64867410 | MS.gene79677:CDS |
| GATCCTAATTCTCATATTTG+CGG | 0.403823 | 3.1:-64866806 | MS.gene79677:CDS |
| CTTGCTCATACAATTTGGAT+TGG | 0.409860 | 3.1:-64865912 | MS.gene79677:CDS |
| TCTAAAAGATTAAAGGTTGA+AGG | 0.413154 | 3.1:+64866411 | None:intergenic |
| TCTTCATTCTAAATGTGGTA+TGG | 0.429366 | 3.1:-64867433 | MS.gene79677:CDS |
| AACAAAGATGGGTGATGGGT+TGG | 0.432814 | 3.1:+64867704 | None:intergenic |
| TTGCTCATACAATTTGGATT+GGG | 0.435090 | 3.1:-64865911 | MS.gene79677:CDS |
| AAACTAGCAGCCTGCATAAG+AGG | 0.442374 | 3.1:+64865639 | None:intergenic |
| GAAGAGGGAGAGAACAAAGA+TGG | 0.445985 | 3.1:+64867692 | None:intergenic |
| CTTTGCACACCCTCTTATGC+AGG | 0.452520 | 3.1:-64865649 | MS.gene79677:CDS |
| GATTAAAGGTTGAAGGATAT+AGG | 0.458002 | 3.1:+64866418 | None:intergenic |
| AAGAGGGAGAGAACAAAGAT+GGG | 0.464589 | 3.1:+64867693 | None:intergenic |
| ATGGCTAGCCTACGGAATAC+TGG | 0.465850 | 3.1:-64866769 | MS.gene79677:CDS |
| GTATGGATTGATAACTCAAC+TGG | 0.468553 | 3.1:-64867416 | MS.gene79677:CDS |
| CATCTATTTGGTGCTTGGAA+TGG | 0.469784 | 3.1:-64865846 | MS.gene79677:CDS |
| GATTAATACCTGTGGATGCC+TGG | 0.472549 | 3.1:+64866329 | None:intergenic |
| AATACTGGTGAGAAAATCAT+TGG | 0.476132 | 3.1:-64866754 | MS.gene79677:CDS |
| GATGAATCCCCGGAGTACTC+TGG | 0.479621 | 3.1:+64866478 | None:intergenic |
| TGGCAGCTCCAGAGTACTCC+GGG | 0.483611 | 3.1:-64866486 | MS.gene79677:CDS |
| CAACAATACCTCGTGAGAAT+CGG | 0.485418 | 3.1:+64867355 | None:intergenic |
| ATTTGGAGATGAGAAGTATG+AGG | 0.486179 | 3.1:+64867628 | None:intergenic |
| CTGCTAGTTTCAACCTCCAT+TGG | 0.497102 | 3.1:-64865626 | MS.gene79677:CDS |
| GAGAGAACAAAGATGGGTGA+TGG | 0.501885 | 3.1:+64867699 | None:intergenic |
| TTGGTGCTTGGAATGGAGAT+AGG | 0.502563 | 3.1:-64865839 | MS.gene79677:CDS |
| AATCCGCAGGTAATCTTTGA+CGG | 0.502619 | 3.1:+64865733 | None:intergenic |
| AACCTGTGAGAGAGAGATAG+TGG | 0.513143 | 3.1:+64867593 | None:intergenic |
| GGTTCTCTTCATTCTAAATG+TGG | 0.519664 | 3.1:-64867438 | MS.gene79677:CDS |
| CATCAAGGATTGCTGCAAAA+GGG | 0.524831 | 3.1:+64865757 | None:intergenic |
| ATTTCCTTTCCACCTACCAA+TGG | 0.531822 | 3.1:+64865610 | None:intergenic |
| GGAATATGAAATGGAAGAAG+AGG | 0.534793 | 3.1:+64867676 | None:intergenic |
| ACAATAGTAGTGACAGCTAA+AGG | 0.535492 | 3.1:+64866691 | None:intergenic |
| ATTACCTGCGGATTTCTACA+AGG | 0.539323 | 3.1:-64865724 | MS.gene79677:CDS |
| TAGTTTCAACCTCCATTGGT+AGG | 0.542093 | 3.1:-64865622 | None:intergenic |
| CACAGAAGTTGATGAATCCC+CGG | 0.546615 | 3.1:+64866468 | None:intergenic |
| GTAATCTTTGACGGCCATCA+AGG | 0.548368 | 3.1:+64865742 | None:intergenic |
| AGAGAACAAAGATGGGTGAT+GGG | 0.548569 | 3.1:+64867700 | None:intergenic |
| AACTCCTTGTAGAAATCCGC+AGG | 0.550585 | 3.1:+64865720 | None:intergenic |
| AAGAATAACCAGGCATCCAC+AGG | 0.553400 | 3.1:-64866337 | MS.gene79677:intron |
| CGGGGATTCATCAACTTCTG+TGG | 0.555871 | 3.1:-64866467 | MS.gene79677:CDS |
| GGGAAAAGACTCGAGCTTCG+TGG | 0.557931 | 3.1:-64867537 | MS.gene79677:CDS |
| TCCTTTCCACCTACCAATGG+AGG | 0.559081 | 3.1:+64865613 | None:intergenic |
| GGAAAAGACTCGAGCTTCGT+GGG | 0.560130 | 3.1:-64867536 | MS.gene79677:CDS |
| GATATGATGGAATTCAACTC+TGG | 0.560955 | 3.1:-64866506 | MS.gene79677:CDS |
| CTGGCAGCTCCAGAGTACTC+CGG | 0.564920 | 3.1:-64866487 | MS.gene79677:CDS |
| AACCACTATCTCTCTCTCAC+AGG | 0.578529 | 3.1:-64867595 | MS.gene79677:CDS |
| AACTAGCAGCCTGCATAAGA+GGG | 0.580769 | 3.1:+64865640 | None:intergenic |
| GAATATGAAATGGAAGAAGA+GGG | 0.585671 | 3.1:+64867677 | None:intergenic |
| GTAATTACTGCATTAACACT+AGG | 0.589570 | 3.1:-64865678 | MS.gene79677:CDS |
| AGAAGCAGAGAGTATAAGAG+AGG | 0.598033 | 3.1:+64867733 | None:intergenic |
| CTGGCATCATAAGAATAACC+AGG | 0.600338 | 3.1:-64866347 | MS.gene79677:CDS |
| TTCCGTTAAACTAAAAGCTG+CGG | 0.611809 | 3.1:+64867505 | None:intergenic |
| ACAGTGGCATGGCTAGCCTA+CGG | 0.614954 | 3.1:-64866777 | MS.gene79677:CDS |
| AGGCGATTAGCTCAAAGACA+TGG | 0.626170 | 3.1:-64865819 | MS.gene79677:CDS |
| GAGAACCACACCCAAAATTG+CGG | 0.633481 | 3.1:+64867454 | None:intergenic |
| AGGGCTCTCCGATTCTCACG+AGG | 0.645915 | 3.1:-64867363 | MS.gene79677:intron |
| TGGCCGTCAAAGATTACCTG+CGG | 0.666868 | 3.1:-64865736 | MS.gene79677:CDS |
| AATTTGGATTGGGAATTCTG+TGG | 0.685087 | 3.1:-64865901 | MS.gene79677:CDS |
| GGCAGCTCCAGAGTACTCCG+GGG | 0.727870 | 3.1:-64866485 | MS.gene79677:CDS |
| GTAGGCTAGCCATGCCACTG+TGG | 0.748430 | 3.1:+64866779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTAATAGATATAGATATA+AGG | + | chr3.1:64867221-64867240 | None:intergenic | 15.0% |
| !! | TTAACATTTCAATATGATAA+CGG | - | chr3.1:64866766-64866785 | MS.gene79677:CDS | 15.0% |
| !! | AAAATACATCTATATGAAAC+TGG | - | chr3.1:64866997-64867016 | MS.gene79677:intron | 20.0% |
| !! | ATAAACTCTTGGAAAATTTA+TGG | - | chr3.1:64866169-64866188 | MS.gene79677:intron | 20.0% |
| !! | TTAATTTCACATCATCTATT+TGG | - | chr3.1:64867505-64867524 | MS.gene79677:CDS | 20.0% |
| !! | TTTATTAATCACAGATATGA+TGG | - | chr3.1:64866844-64866863 | MS.gene79677:intron | 20.0% |
| !!! | AAATTTGAAACGATTGTTAA+TGG | + | chr3.1:64866055-64866074 | None:intergenic | 20.0% |
| !!! | AATTATATGTTGTTTCTCTT+TGG | + | chr3.1:64866308-64866327 | None:intergenic | 20.0% |
| !!! | GTTTCATATAGATGTATTTT+AGG | + | chr3.1:64866997-64867016 | None:intergenic | 20.0% |
| !!! | TGCAATTTCTAAAAGATTAA+AGG | + | chr3.1:64866962-64866981 | None:intergenic | 20.0% |
| ! | AATGCAGTAATTACTAAGTA+TGG | + | chr3.1:64867679-64867698 | None:intergenic | 25.0% |
| ! | ACAAATTTATCACGTATTTG+AGG | + | chr3.1:64867302-64867321 | None:intergenic | 25.0% |
| ! | ACTCTATACCAATAAGTTTA+TGG | - | chr3.1:64867062-64867081 | MS.gene79677:intron | 25.0% |
| ! | ATGATTAGGAGAAAAGATAT+GGG | + | chr3.1:64866714-64866733 | None:intergenic | 25.0% |
| ! | ATGTCTATCCATAAACTTAT+TGG | + | chr3.1:64867073-64867092 | None:intergenic | 25.0% |
| ! | ATTACCCATTCTTCAATAAA+GGG | - | chr3.1:64865806-64865825 | MS.gene79677:CDS | 25.0% |
| ! | GAAAAAGAAAGAGATGAAAT+TGG | + | chr3.1:64866926-64866945 | None:intergenic | 25.0% |
| ! | TATGATTAGGAGAAAAGATA+TGG | + | chr3.1:64866715-64866734 | None:intergenic | 25.0% |
| ! | TCTAAAAGATTAAAGGTTGA+AGG | + | chr3.1:64866955-64866974 | None:intergenic | 25.0% |
| ! | TTAGACACATTTCTTTCATT+TGG | - | chr3.1:64867364-64867383 | MS.gene79677:CDS | 25.0% |
| !! | TCTAATATTTTGTCGTAAGA+AGG | - | chr3.1:64867147-64867166 | MS.gene79677:intron | 25.0% |
| !! | TTTTCCCTTTATTGAAGAAT+GGG | + | chr3.1:64865813-64865832 | None:intergenic | 25.0% |
| !!! | AGTGGTTTTGAATTTGAATT+TGG | + | chr3.1:64865755-64865774 | None:intergenic | 25.0% |
| !!! | GCTCATTTGTTATTTATGTT+AGG | - | chr3.1:64866091-64866110 | MS.gene79677:intron | 25.0% |
| !!! | TCAATATGATAACGGTTTAA+CGG | - | chr3.1:64866774-64866793 | MS.gene79677:CDS | 25.0% |
| !!! | TCGAAATATGTGATTTTGAT+GGG | - | chr3.1:64866372-64866391 | MS.gene79677:CDS | 25.0% |
| !!! | TTTCTATTTAGAAGAGCATT+TGG | + | chr3.1:64867325-64867344 | None:intergenic | 25.0% |
| !!! | TTTTGGTTTGGAATATGAAA+TGG | + | chr3.1:64865699-64865718 | None:intergenic | 25.0% |
| AAACCGCAAATATGAGAATT+AGG | + | chr3.1:64866563-64866582 | None:intergenic | 30.0% | |
| AAATCAATTCAGATACTCTC+TGG | + | chr3.1:64866346-64866365 | None:intergenic | 30.0% | |
| AATACTGGTGAGAAAATCAT+TGG | - | chr3.1:64866609-64866628 | MS.gene79677:intron | 30.0% | |
| AGATTGTTTGAGTGAACTTA+TGG | + | chr3.1:64866223-64866242 | None:intergenic | 30.0% | |
| AGCATTTCGCTTATATGATT+AGG | + | chr3.1:64866728-64866747 | None:intergenic | 30.0% | |
| AGGATATAATGTTACTCTTG+AGG | + | chr3.1:64867269-64867288 | None:intergenic | 30.0% | |
| AGTGTTAAGATTAATACCTG+TGG | + | chr3.1:64867045-64867064 | None:intergenic | 30.0% | |
| ATAAAATCAGGTTTGGGTAT+TGG | + | chr3.1:64866419-64866438 | None:intergenic | 30.0% | |
| CATTACCCATTCTTCAATAA+AGG | - | chr3.1:64865805-64865824 | MS.gene79677:CDS | 30.0% | |
| GAAATGTGTCTAAATAGAGA+AGG | + | chr3.1:64867357-64867376 | None:intergenic | 30.0% | |
| GAATATGAAATGGAAGAAGA+GGG | + | chr3.1:64865689-64865708 | None:intergenic | 30.0% | |
| GATCCTAATTCTCATATTTG+CGG | - | chr3.1:64866557-64866576 | MS.gene79677:intron | 30.0% | |
| GATTAAAGGTTGAAGGATAT+AGG | + | chr3.1:64866948-64866967 | None:intergenic | 30.0% | |
| GTAATTACTGCATTAACACT+AGG | - | chr3.1:64867685-64867704 | MS.gene79677:CDS | 30.0% | |
| TCTTCATTCTAAATGTGGTA+TGG | - | chr3.1:64865930-64865949 | MS.gene79677:CDS | 30.0% | |
| TGAGATTTCGAGATAAAATC+AGG | + | chr3.1:64866431-64866450 | None:intergenic | 30.0% | |
| TGTTAATGGTGAAGTGAAAA+GGG | + | chr3.1:64866041-64866060 | None:intergenic | 30.0% | |
| TTCGAGATAAAATCAGGTTT+GGG | + | chr3.1:64866425-64866444 | None:intergenic | 30.0% | |
| TTGCTCATACAATTTGGATT+GGG | - | chr3.1:64867452-64867471 | MS.gene79677:CDS | 30.0% | |
| TTGTTAATGGTGAAGTGAAA+AGG | + | chr3.1:64866042-64866061 | None:intergenic | 30.0% | |
| TTTCGAGATAAAATCAGGTT+TGG | + | chr3.1:64866426-64866445 | None:intergenic | 30.0% | |
| ! | ATGGATAGACATGATTTTTG+TGG | - | chr3.1:64867081-64867100 | MS.gene79677:intron | 30.0% |
| ! | ATTTATTTCGCCGCAATTTT+GGG | - | chr3.1:64865899-64865918 | MS.gene79677:CDS | 30.0% |
| ! | CTCGAAATATGTGATTTTGA+TGG | - | chr3.1:64866371-64866390 | MS.gene79677:CDS | 30.0% |
| ! | CTTTTCCCTTTATTGAAGAA+TGG | + | chr3.1:64865814-64865833 | None:intergenic | 30.0% |
| ! | GCGAAATGCTTTACTTATTT+TGG | - | chr3.1:64866738-64866757 | MS.gene79677:CDS | 30.0% |
| ! | TGATTTTTCATCTATGATGC+AGG | - | chr3.1:64866816-64866835 | MS.gene79677:CDS | 30.0% |
| !! | AAGCTGTTTTCATAAACTCT+TGG | - | chr3.1:64866158-64866177 | MS.gene79677:intron | 30.0% |
| !! | GATTTTGATGGGAAAAATAC+TGG | - | chr3.1:64866383-64866402 | MS.gene79677:CDS | 30.0% |
| ACAATAGTAGTGACAGCTAA+AGG | + | chr3.1:64866675-64866694 | None:intergenic | 35.0% | |
| CGGAACAGAAAATAACTTCA+TGG | - | chr3.1:64865876-64865895 | MS.gene79677:CDS | 35.0% | |
| CTTGCTCATACAATTTGGAT+TGG | - | chr3.1:64867451-64867470 | MS.gene79677:CDS | 35.0% | |
| GGAATATGAAATGGAAGAAG+AGG | + | chr3.1:64865690-64865709 | None:intergenic | 35.0% | |
| GGTTCTCTTCATTCTAAATG+TGG | - | chr3.1:64865925-64865944 | MS.gene79677:CDS | 35.0% | |
| TTCCGTTAAACTAAAAGCTG+CGG | + | chr3.1:64865861-64865880 | None:intergenic | 35.0% | |
| ! | AATTTGGATTGGGAATTCTG+TGG | - | chr3.1:64867462-64867481 | MS.gene79677:CDS | 35.0% |
| ! | ATTGATAACTCAACTGGCTT+TGG | - | chr3.1:64865953-64865972 | MS.gene79677:intron | 35.0% |
| ! | ATTTGGAGATGAGAAGTATG+AGG | + | chr3.1:64865738-64865757 | None:intergenic | 35.0% |
| ! | GATATGATGGAATTCAACTC+TGG | - | chr3.1:64866857-64866876 | MS.gene79677:intron | 35.0% |
| ! | GATTTATTTCGCCGCAATTT+TGG | - | chr3.1:64865898-64865917 | MS.gene79677:CDS | 35.0% |
| ! | GTATGGATTGATAACTCAAC+TGG | - | chr3.1:64865947-64865966 | MS.gene79677:intron | 35.0% |
| ! | GTATTTGAGGATGTTTTACG+AGG | + | chr3.1:64867289-64867308 | None:intergenic | 35.0% |
| ! | TCATTGGTGAAAGAGCTTTT+CGG | - | chr3.1:64866625-64866644 | MS.gene79677:intron | 35.0% |
| !! | ATGTTGTTTCTCTTTGGCTA+TGG | + | chr3.1:64866302-64866321 | None:intergenic | 35.0% |
| !! | TGTTGTTTCTCTTTGGCTAT+GGG | + | chr3.1:64866301-64866320 | None:intergenic | 35.0% |
| !!! | CTTTTCGGGTTATTTTTGCA+GGG | - | chr3.1:64866640-64866659 | MS.gene79677:intron | 35.0% |
| !!! | CTTTTGTTGATGCTGTTTCA+GGG | - | chr3.1:64865981-64866000 | MS.gene79677:intron | 35.0% |
| !!! | GATTTGTTTTGCTGTTCAGA+TGG | - | chr3.1:64867408-64867427 | MS.gene79677:CDS | 35.0% |
| !!! | TTGAGAAGTAGGTTTTGGTT+TGG | + | chr3.1:64865711-64865730 | None:intergenic | 35.0% |
| AAGAGGGAGAGAACAAAGAT+GGG | + | chr3.1:64865673-64865692 | None:intergenic | 40.0% | |
| AATCCGCAGGTAATCTTTGA+CGG | + | chr3.1:64867633-64867652 | None:intergenic | 40.0% | |
| AGAGAACAAAGATGGGTGAT+GGG | + | chr3.1:64865666-64865685 | None:intergenic | 40.0% | |
| ATTACCTGCGGATTTCTACA+AGG | - | chr3.1:64867639-64867658 | MS.gene79677:CDS | 40.0% | |
| CAACAATACCTCGTGAGAAT+CGG | + | chr3.1:64866011-64866030 | None:intergenic | 40.0% | |
| CATCAAGGATTGCTGCAAAA+GGG | + | chr3.1:64867609-64867628 | None:intergenic | 40.0% | |
| CTGGCATCATAAGAATAACC+AGG | - | chr3.1:64867016-64867035 | MS.gene79677:intron | 40.0% | |
| GGTGTCTTGCTCATACAATT+TGG | - | chr3.1:64867446-64867465 | MS.gene79677:CDS | 40.0% | |
| ! | ACTCTTGAGGATGTTTTACG+AGG | + | chr3.1:64867256-64867275 | None:intergenic | 40.0% |
| ! | AGAAGCAGAGAGTATAAGAG+AGG | + | chr3.1:64865633-64865652 | None:intergenic | 40.0% |
| ! | ATATTTGCGGTTTTCCACAG+TGG | - | chr3.1:64866570-64866589 | MS.gene79677:intron | 40.0% |
| ! | CATTGGTGAAAGAGCTTTTC+GGG | - | chr3.1:64866626-64866645 | MS.gene79677:intron | 40.0% |
| ! | GTTTCAATTGCTCTGAGTGA+AGG | - | chr3.1:64867386-64867405 | MS.gene79677:CDS | 40.0% |
| ! | TTTTCTCACCAGTATTCCGT+AGG | + | chr3.1:64866605-64866624 | None:intergenic | 40.0% |
| !! | CACATCATCTATTTGGTGCT+TGG | - | chr3.1:64867512-64867531 | MS.gene79677:CDS | 40.0% |
| !! | CATCTATTTGGTGCTTGGAA+TGG | - | chr3.1:64867517-64867536 | MS.gene79677:CDS | 40.0% |
| !! | CTGCATTAGTCGTTGATTTG+TGG | - | chr3.1:64866497-64866516 | MS.gene79677:CDS | 40.0% |
| !!! | CTCCGCAGCTTTTAGTTTAA+CGG | - | chr3.1:64865856-64865875 | MS.gene79677:CDS | 40.0% |
| !!! | GCTTTTCGGGTTATTTTTGC+AGG | - | chr3.1:64866639-64866658 | MS.gene79677:intron | 40.0% |
| !!! | GCTTTTGTTGATGCTGTTTC+AGG | - | chr3.1:64865980-64865999 | MS.gene79677:intron | 40.0% |
| !!! | TGTTTTGCTGTTCAGATGGT+TGG | - | chr3.1:64867412-64867431 | MS.gene79677:CDS | 40.0% |
| AAACTAGCAGCCTGCATAAG+AGG | + | chr3.1:64867727-64867746 | None:intergenic | 45.0% | |
| AACCACTATCTCTCTCTCAC+AGG | - | chr3.1:64865768-64865787 | MS.gene79677:CDS | 45.0% | |
| AACCTGTGAGAGAGAGATAG+TGG | + | chr3.1:64865773-64865792 | None:intergenic | 45.0% | |
| AACTAGCAGCCTGCATAAGA+GGG | + | chr3.1:64867726-64867745 | None:intergenic | 45.0% | |
| AACTCCTTGTAGAAATCCGC+AGG | + | chr3.1:64867646-64867665 | None:intergenic | 45.0% | |
| AAGAATAACCAGGCATCCAC+AGG | - | chr3.1:64867026-64867045 | MS.gene79677:intron | 45.0% | |
| ACCTGCAGAGTAACATGTCA+AGG | + | chr3.1:64866528-64866547 | None:intergenic | 45.0% | |
| CCATCAAGGATTGCTGCAAA+AGG | + | chr3.1:64867610-64867629 | None:intergenic | 45.0% | |
| CCTGCAGAGTAACATGTCAA+GGG | + | chr3.1:64866527-64866546 | None:intergenic | 45.0% | |
| GAAGAGGGAGAGAACAAAGA+TGG | + | chr3.1:64865674-64865693 | None:intergenic | 45.0% | |
| GAGAACCACACCCAAAATTG+CGG | + | chr3.1:64865912-64865931 | None:intergenic | 45.0% | |
| GAGAGAACAAAGATGGGTGA+TGG | + | chr3.1:64865667-64865686 | None:intergenic | 45.0% | |
| GATTAATACCTGTGGATGCC+TGG | + | chr3.1:64867037-64867056 | None:intergenic | 45.0% | |
| GTAATCTTTGACGGCCATCA+AGG | + | chr3.1:64867624-64867643 | None:intergenic | 45.0% | |
| TCTCTCACAGGTTCAACTTC+AGG | - | chr3.1:64865780-64865799 | MS.gene79677:CDS | 45.0% | |
| TTGACATGTTACTCTGCAGG+TGG | - | chr3.1:64866527-64866546 | MS.gene79677:CDS | 45.0% | |
| ! | AGATGGTTGGTCAGGTAATC+TGG | - | chr3.1:64867425-64867444 | MS.gene79677:CDS | 45.0% |
| ! | AGGCGATTAGCTCAAAGACA+TGG | - | chr3.1:64867544-64867563 | MS.gene79677:CDS | 45.0% |
| ! | CCTTTTGCAGCAATCCTTGA+TGG | - | chr3.1:64867607-64867626 | MS.gene79677:CDS | 45.0% |
| ! | CTGCTAGTTTCAACCTCCAT+TGG | - | chr3.1:64867737-64867756 | MS.gene79677:CDS | 45.0% |
| ! | TTGGTGCTTGGAATGGAGAT+AGG | - | chr3.1:64867524-64867543 | MS.gene79677:CDS | 45.0% |
| !! | AACAAAGATGGGTGATGGGT+TGG | + | chr3.1:64865662-64865681 | None:intergenic | 45.0% |
| !! | CACAGAAGTTGATGAATCCC+CGG | + | chr3.1:64866898-64866917 | None:intergenic | 45.0% |
| !! | TATGAGGAGCGTTGAGAAGT+AGG | + | chr3.1:64865722-64865741 | None:intergenic | 45.0% |
| !!! | GAGCGTTGAGAAGTAGGTTT+TGG | + | chr3.1:64865716-64865735 | None:intergenic | 45.0% |
| CCCTTGACATGTTACTCTGC+AGG | - | chr3.1:64866524-64866543 | MS.gene79677:CDS | 50.0% | |
| CGGGGATTCATCAACTTCTG+TGG | - | chr3.1:64866896-64866915 | MS.gene79677:intron | 50.0% | |
| CTTTGCACACCCTCTTATGC+AGG | - | chr3.1:64867714-64867733 | MS.gene79677:CDS | 50.0% | |
| GGAAAAGACTCGAGCTTCGT+GGG | - | chr3.1:64865827-64865846 | MS.gene79677:CDS | 50.0% | |
| GTGGCTGTTGCAGCTTCAAT+TGG | - | chr3.1:64867481-64867500 | MS.gene79677:CDS | 50.0% | |
| TGGCCGTCAAAGATTACCTG+CGG | - | chr3.1:64867627-64867646 | MS.gene79677:CDS | 50.0% | |
| ! | TGCTGTTCAGATGGTTGGTC+AGG | - | chr3.1:64867417-64867436 | MS.gene79677:CDS | 50.0% |
| !! | ATGGCTAGCCTACGGAATAC+TGG | - | chr3.1:64866594-64866613 | MS.gene79677:intron | 50.0% |
| !! | TTTCGCCGCAATTTTGGGTG+TGG | - | chr3.1:64865904-64865923 | MS.gene79677:CDS | 50.0% |
| GGGAAAAGACTCGAGCTTCG+TGG | - | chr3.1:64865826-64865845 | MS.gene79677:CDS | 55.0% | |
| ! | GATGAATCCCCGGAGTACTC+TGG | + | chr3.1:64866888-64866907 | None:intergenic | 55.0% |
| ! | TGCGGTTTTCCACAGTGGCA+TGG | - | chr3.1:64866575-64866594 | MS.gene79677:intron | 55.0% |
| !! | ACAGTGGCATGGCTAGCCTA+CGG | - | chr3.1:64866586-64866605 | MS.gene79677:intron | 55.0% |
| AGGGCTCTCCGATTCTCACG+AGG | - | chr3.1:64866000-64866019 | MS.gene79677:intron | 60.0% | |
| ! | CTGGCAGCTCCAGAGTACTC+CGG | - | chr3.1:64866876-64866895 | MS.gene79677:intron | 60.0% |
| ! | GTAGGCTAGCCATGCCACTG+TGG | + | chr3.1:64866587-64866606 | None:intergenic | 60.0% |
| ! | TGGCAGCTCCAGAGTACTCC+GGG | - | chr3.1:64866877-64866896 | MS.gene79677:intron | 60.0% |
| ! | GGCAGCTCCAGAGTACTCCG+GGG | - | chr3.1:64866878-64866897 | MS.gene79677:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 64865623 | 64867762 | 64865623 | ID=MS.gene79677 |
| chr3.1 | mRNA | 64865623 | 64867762 | 64865623 | ID=MS.gene79677.t1;Parent=MS.gene79677 |
| chr3.1 | exon | 64867364 | 64867762 | 64867364 | ID=MS.gene79677.t1.exon1;Parent=MS.gene79677.t1 |
| chr3.1 | CDS | 64867364 | 64867762 | 64867364 | ID=cds.MS.gene79677.t1;Parent=MS.gene79677.t1 |
| chr3.1 | exon | 64866693 | 64866839 | 64866693 | ID=MS.gene79677.t1.exon2;Parent=MS.gene79677.t1 |
| chr3.1 | CDS | 64866693 | 64866839 | 64866693 | ID=cds.MS.gene79677.t1;Parent=MS.gene79677.t1 |
| chr3.1 | exon | 64866338 | 64866547 | 64866338 | ID=MS.gene79677.t1.exon3;Parent=MS.gene79677.t1 |
| chr3.1 | CDS | 64866338 | 64866547 | 64866338 | ID=cds.MS.gene79677.t1;Parent=MS.gene79677.t1 |
| chr3.1 | exon | 64865623 | 64865958 | 64865623 | ID=MS.gene79677.t1.exon4;Parent=MS.gene79677.t1 |
| chr3.1 | CDS | 64865623 | 64865958 | 64865623 | ID=cds.MS.gene79677.t1;Parent=MS.gene79677.t1 |
| Gene Sequence |
| Protein sequence |
