Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005556.01.T01 | KEH43766.1 | 89.011 | 182 | 14 | 1 | 22 | 203 | 23 | 198 | 1.48E-103 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005556.01.T01 | P11432 | 78.325 | 203 | 37 | 1 | 1 | 203 | 1 | 196 | 6.33E-104 | 300 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005556.01.T01 | A0A396JXB1 | 89.011 | 182 | 14 | 1 | 22 | 203 | 23 | 198 | 7.09e-104 | 306 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048273.01 | MsG0180005556.01 | 0.802678 | 5.005234e-49 | 2.466815e-46 |
| MsG0080048887.01 | MsG0180005556.01 | 0.819169 | 1.337539e-52 | 1.021582e-49 |
| MsG0080049072.01 | MsG0180005556.01 | 0.807276 | 5.470212e-50 | 3.034093e-47 |
| MsG0080049129.01 | MsG0180005556.01 | 0.842008 | 3.359921e-58 | 5.025648e-55 |
| MsG0180000412.01 | MsG0180005556.01 | 0.807429 | 5.077481e-50 | 2.827414e-47 |
| MsG0180000423.01 | MsG0180005556.01 | 0.807209 | 5.652740e-50 | 3.129854e-47 |
| MsG0180000490.01 | MsG0180005556.01 | 0.810982 | 8.789252e-51 | 5.376255e-48 |
| MsG0180001615.01 | MsG0180005556.01 | 0.807068 | 6.054661e-50 | 3.340021e-47 |
| MsG0180003196.01 | MsG0180005556.01 | 0.808730 | 2.683210e-50 | 1.545846e-47 |
| MsG0180003352.01 | MsG0180005556.01 | 0.851612 | 7.921826e-61 | 1.612345e-57 |
| MsG0180003617.01 | MsG0180005556.01 | 0.845993 | 2.870412e-59 | 4.870474e-56 |
| MsG0180003933.01 | MsG0180005556.01 | 0.811982 | 5.331556e-51 | 3.348229e-48 |
| MsG0180004662.01 | MsG0180005556.01 | 0.862219 | 5.915686e-64 | 1.719431e-60 |
| MsG0180004792.01 | MsG0180005556.01 | 0.816936 | 4.276915e-52 | 3.072308e-49 |
| MsG0180005338.01 | MsG0180005556.01 | 0.810482 | 1.127503e-50 | 6.804349e-48 |
| MsG0180005416.01 | MsG0180005556.01 | 0.808940 | 2.419846e-50 | 1.401891e-47 |
| MsG0180005545.01 | MsG0180005556.01 | 0.963285 | 6.724909e-122 | 3.591342e-116 |
| MsG0180005555.01 | MsG0180005556.01 | 0.970310 | 2.013570e-131 | 1.983884e-125 |
| MsG0180005556.01 | MsG0180005557.01 | 0.950297 | 2.202592e-108 | 4.644486e-103 |
| MsG0180005556.01 | MsG0180005558.01 | 0.953904 | 9.766213e-112 | 2.650435e-106 |
| MsG0180005556.01 | MsG0180005672.01 | 0.816835 | 4.505974e-52 | 3.227762e-49 |
| MsG0180005556.01 | MsG0180005898.01 | 0.854414 | 1.251042e-61 | 2.791614e-58 |
| MsG0180005556.01 | MsG0180006217.01 | 0.808925 | 2.436744e-50 | 1.411160e-47 |
| MsG0180005556.01 | MsG0280006319.01 | 0.851476 | 8.657727e-61 | 1.754351e-57 |
| MsG0180005556.01 | MsG0280006402.01 | 0.831322 | 1.782666e-55 | 1.927817e-52 |
| MsG0180005556.01 | MsG0280007423.01 | 0.893674 | 5.042660e-75 | 4.852903e-71 |
| MsG0180005556.01 | MsG0280007511.01 | 0.817793 | 2.742358e-52 | 2.016728e-49 |
| MsG0180005556.01 | MsG0280007783.01 | 0.812656 | 3.798714e-51 | 2.429173e-48 |
| MsG0180005556.01 | MsG0280009287.01 | 0.814519 | 1.478083e-51 | 9.940126e-49 |
| MsG0180005556.01 | MsG0280009863.01 | 0.865085 | 7.625488e-65 | 2.450272e-61 |
| MsG0180005556.01 | MsG0280010496.01 | 0.813608 | 2.348072e-51 | 1.540538e-48 |
| MsG0180005556.01 | MsG0280010728.01 | 0.805486 | 1.304558e-49 | 6.910262e-47 |
| MsG0180005556.01 | MsG0280011169.01 | 0.809501 | 1.834572e-50 | 1.078917e-47 |
| MsG0180005556.01 | MsG0280011189.01 | 0.806492 | 8.014523e-50 | 4.355989e-47 |
| MsG0180005556.01 | MsG0280011207.01 | 0.813244 | 2.824167e-51 | 1.834432e-48 |
| MsG0180005556.01 | MsG0380012065.01 | 0.814305 | 1.648649e-51 | 1.102303e-48 |
| MsG0180005556.01 | MsG0380013501.01 | 0.819751 | 9.853208e-53 | 7.649018e-50 |
| MsG0180005556.01 | MsG0380014646.01 | 0.876821 | 1.029188e-68 | 5.061274e-65 |
| MsG0180005556.01 | MsG0380015129.01 | 0.806705 | 7.225584e-50 | 3.948620e-47 |
| MsG0180005556.01 | MsG0380015496.01 | 0.808789 | 2.605593e-50 | 1.503497e-47 |
| MsG0180005556.01 | MsG0380015744.01 | 0.841928 | 3.528177e-58 | 5.264022e-55 |
| MsG0180005556.01 | MsG0380015828.01 | 0.838573 | 2.654629e-57 | 3.570292e-54 |
| MsG0180005556.01 | MsG0380015974.01 | 0.801543 | 8.566683e-49 | 4.102000e-46 |
| MsG0180005556.01 | MsG0380016065.01 | 0.824333 | 8.545352e-54 | 7.545924e-51 |
| MsG0180005556.01 | MsG0380016066.01 | 0.883689 | 3.625934e-71 | 2.323048e-67 |
| MsG0180005556.01 | MsG0380016151.01 | 0.828787 | 7.402162e-55 | 7.432298e-52 |
| MsG0180005556.01 | MsG0380016354.01 | 0.810706 | 1.008722e-50 | 6.124222e-48 |
| MsG0180005556.01 | MsG0380016456.01 | 0.806527 | 7.876962e-50 | 4.285088e-47 |
| MsG0180005556.01 | MsG0380016492.01 | 0.816988 | 4.163451e-52 | 2.995106e-49 |
| MsG0180005556.01 | MsG0380016751.01 | 0.811059 | 8.459998e-51 | 5.184880e-48 |
| MsG0180005556.01 | MsG0380016762.01 | 0.803553 | 3.300188e-49 | 1.663405e-46 |
| MsG0180005556.01 | MsG0380016861.01 | 0.800426 | 1.449404e-48 | 6.746997e-46 |
| MsG0180005556.01 | MsG0380016936.01 | 0.874525 | 6.310840e-68 | 2.849664e-64 |
| MsG0180005556.01 | MsG0380016997.01 | 0.864936 | 8.491800e-65 | 2.713392e-61 |
| MsG0180005556.01 | MsG0380017353.01 | 0.844309 | 8.189348e-59 | 1.316714e-55 |
| MsG0180005556.01 | MsG0380017532.01 | 0.871154 | 8.482651e-67 | 3.386264e-63 |
| MsG0180005556.01 | MsG0380017666.01 | -0.858889 | 6.040297e-63 | 1.565341e-59 |
| MsG0180005556.01 | MsG0380017918.01 | 0.899519 | 1.828650e-77 | 2.269712e-73 |
| MsG0180005556.01 | MsG0480018629.01 | 0.839157 | 1.874509e-57 | 2.566182e-54 |
| MsG0180005556.01 | MsG0480018905.01 | 0.804894 | 1.735091e-49 | 9.053073e-47 |
| MsG0180005556.01 | MsG0480019573.01 | 0.814315 | 1.640203e-51 | 1.096946e-48 |
| MsG0180005556.01 | MsG0480020027.01 | 0.869137 | 3.879601e-66 | 1.440078e-62 |
| MsG0180005556.01 | MsG0480020704.01 | 0.833287 | 5.817000e-56 | 6.668730e-53 |
| MsG0180005556.01 | MsG0480020938.01 | 0.818999 | 1.462069e-52 | 1.111628e-49 |
| MsG0180005556.01 | MsG0480021151.01 | 0.833759 | 4.435320e-56 | 5.157178e-53 |
| MsG0180005556.01 | MsG0480021483.01 | 0.806000 | 1.017200e-49 | 5.460037e-47 |
| MsG0180005556.01 | MsG0480021999.01 | 0.808431 | 3.107741e-50 | 1.776661e-47 |
| MsG0180005556.01 | MsG0480022002.01 | 0.805158 | 1.528057e-49 | 8.026265e-47 |
| MsG0180005556.01 | MsG0480022242.01 | 0.824691 | 7.036870e-54 | 6.279463e-51 |
| MsG0180005556.01 | MsG0480022244.01 | 0.836101 | 1.140097e-56 | 1.422321e-53 |
| MsG0180005556.01 | MsG0480022805.01 | 0.863548 | 2.301308e-64 | 7.002910e-61 |
| MsG0180005556.01 | MsG0480023125.01 | -0.813171 | 2.928890e-51 | 1.898740e-48 |
| MsG0180005556.01 | MsG0480023173.01 | 0.817677 | 2.913538e-52 | 2.135709e-49 |
| MsG0180005556.01 | MsG0480023375.01 | 0.822209 | 2.677270e-53 | 2.226282e-50 |
| MsG0180005556.01 | MsG0480023386.01 | 0.808729 | 2.684441e-50 | 1.546520e-47 |
| MsG0180005556.01 | MsG0480023395.01 | 0.829770 | 4.274415e-55 | 4.417947e-52 |
| MsG0180005556.01 | MsG0480023466.01 | 0.804151 | 2.479242e-49 | 1.268929e-46 |
| MsG0180005556.01 | MsG0480023505.01 | 0.804551 | 2.046219e-49 | 1.058079e-46 |
| MsG0180005556.01 | MsG0580024386.01 | 0.842622 | 2.310598e-58 | 3.523374e-55 |
| MsG0180005556.01 | MsG0580024471.01 | 0.874221 | 7.999232e-68 | 3.571516e-64 |
| MsG0180005556.01 | MsG0580024567.01 | 0.867852 | 1.008292e-65 | 3.571785e-62 |
| MsG0180005556.01 | MsG0580024763.01 | 0.851744 | 7.269748e-61 | 1.485953e-57 |
| MsG0180005556.01 | MsG0580024764.01 | 0.861866 | 7.588660e-64 | 2.178265e-60 |
| MsG0180005556.01 | MsG0580024766.01 | 0.811356 | 7.294136e-51 | 4.505042e-48 |
| MsG0180005556.01 | MsG0580025097.01 | 0.803668 | 3.124053e-49 | 1.579147e-46 |
| MsG0180005556.01 | MsG0580025252.01 | 0.818816 | 1.608510e-52 | 1.216639e-49 |
| MsG0180005556.01 | MsG0580025413.01 | 0.806938 | 6.450031e-50 | 3.546178e-47 |
| MsG0180005556.01 | MsG0580026362.01 | 0.818317 | 2.087416e-52 | 1.557303e-49 |
| MsG0180005556.01 | MsG0580027254.01 | 0.810835 | 9.460814e-51 | 5.763770e-48 |
| MsG0180005556.01 | MsG0580027803.01 | 0.812328 | 4.481285e-51 | 2.840958e-48 |
| MsG0180005556.01 | MsG0580028013.01 | 0.815582 | 8.586502e-52 | 5.943376e-49 |
| MsG0180005556.01 | MsG0580028925.01 | 0.810056 | 1.393240e-50 | 8.314702e-48 |
| MsG0180005556.01 | MsG0580029719.01 | 0.816892 | 4.375687e-52 | 3.139273e-49 |
| MsG0180005556.01 | MsG0580030063.01 | 0.841042 | 6.039707e-58 | 8.765214e-55 |
| MsG0180005556.01 | MsG0680031204.01 | 0.800253 | 1.571765e-48 | 7.284285e-46 |
| MsG0180005556.01 | MsG0680032245.01 | 0.800703 | 1.272475e-48 | 5.964708e-46 |
| MsG0180005556.01 | MsG0680032769.01 | 0.808397 | 3.160188e-50 | 1.805027e-47 |
| MsG0180005556.01 | MsG0680034724.01 | 0.829557 | 4.816107e-55 | 4.946035e-52 |
| MsG0180005556.01 | MsG0680035612.01 | 0.849891 | 2.416525e-60 | 4.649363e-57 |
| MsG0180005556.01 | MsG0680035664.01 | 0.814871 | 1.235165e-51 | 8.384902e-49 |
| MsG0180005556.01 | MsG0680035665.01 | 0.841026 | 6.099912e-58 | 8.847807e-55 |
| MsG0180005556.01 | MsG0780037963.01 | 0.809795 | 1.585798e-50 | 9.399678e-48 |
| MsG0180005556.01 | MsG0780038416.01 | 0.834208 | 3.423130e-56 | 4.034093e-53 |
| MsG0180005556.01 | MsG0780038573.01 | 0.800449 | 1.433529e-48 | 6.676803e-46 |
| MsG0180005556.01 | MsG0780039805.01 | 0.809350 | 1.976622e-50 | 1.157863e-47 |
| MsG0180005556.01 | MsG0780040350.01 | 0.825351 | 4.915193e-54 | 4.468944e-51 |
| MsG0180005556.01 | MsG0780040670.01 | -0.826574 | 2.518065e-54 | 2.370517e-51 |
| MsG0180005556.01 | MsG0780041807.01 | 0.800115 | 1.676348e-48 | 7.741807e-46 |
| MsG0180005556.01 | MsG0880041908.01 | 0.814121 | 1.810288e-51 | 1.204275e-48 |
| MsG0180005556.01 | MsG0880043056.01 | 0.806360 | 8.544320e-50 | 4.628225e-47 |
| MsG0180005556.01 | MsG0880043515.01 | 0.816985 | 4.169460e-52 | 2.999193e-49 |
| MsG0180005556.01 | MsG0880043870.01 | 0.838841 | 2.262430e-57 | 3.067767e-54 |
| MsG0180005556.01 | MsG0880044105.01 | 0.835316 | 1.801751e-56 | 2.195436e-53 |
| MsG0180005556.01 | MsG0880044317.01 | 0.867310 | 1.504327e-65 | 5.228493e-62 |
| MsG0180005556.01 | MsG0880044318.01 | 0.834228 | 3.383866e-56 | 3.990233e-53 |
| MsG0180005556.01 | MsG0880044411.01 | 0.836371 | 9.734589e-57 | 1.224325e-53 |
| MsG0180005556.01 | MsG0880044436.01 | 0.831688 | 1.448630e-55 | 1.583796e-52 |
| MsG0180005556.01 | MsG0880044453.01 | 0.801620 | 8.264323e-49 | 3.964726e-46 |
| MsG0180005556.01 | MsG0880044545.01 | 0.825791 | 3.865303e-54 | 3.558973e-51 |
| MsG0180005556.01 | MsG0880044548.01 | 0.802160 | 6.401392e-49 | 3.113588e-46 |
| MsG0180005556.01 | MsG0880044834.01 | 0.808637 | 2.808897e-50 | 1.614320e-47 |
| MsG0180005556.01 | MsG0880044884.01 | 0.811998 | 5.287548e-51 | 3.322080e-48 |
| MsG0180005556.01 | MsG0880045703.01 | 0.805907 | 1.063930e-49 | 5.697076e-47 |
| MsG0180005556.01 | MsG0880046160.01 | 0.800561 | 1.360689e-48 | 6.355394e-46 |
| MsG0180005556.01 | MsG0880046324.01 | 0.872216 | 3.771384e-67 | 1.565142e-63 |
| MsG0180005556.01 | MsG0880046408.01 | 0.802082 | 6.642471e-49 | 3.224401e-46 |
| MsG0180005556.01 | MsG0880046838.01 | 0.831757 | 1.392653e-55 | 1.525835e-52 |
| MsG0180005556.01 | MsG0880047207.01 | 0.825883 | 3.675514e-54 | 3.393169e-51 |
| MsG0180005556.01 | MsG0880047435.01 | 0.803910 | 2.782659e-49 | 1.415493e-46 |
| MsG0180005556.01 | MsG0880047451.01 | 0.816373 | 5.718080e-52 | 4.044561e-49 |
| MsG0180005556.01 | MsG0880047568.01 | -0.809324 | 2.001413e-50 | 1.171558e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005556.01.T01 | MTR_1g102570 | 89.011 | 182 | 14 | 1 | 22 | 203 | 23 | 198 | 1.80e-107 | 306 |
| MsG0180005556.01.T01 | MTR_1g102800 | 88.043 | 184 | 16 | 1 | 20 | 203 | 20 | 197 | 3.62e-107 | 306 |
| MsG0180005556.01.T01 | MTR_1g102780 | 89.560 | 182 | 15 | 1 | 22 | 203 | 23 | 200 | 3.09e-101 | 291 |
| MsG0180005556.01.T01 | MTR_1g102830 | 75.824 | 182 | 26 | 2 | 22 | 203 | 23 | 186 | 8.79e-93 | 269 |
| MsG0180005556.01.T01 | MTR_1g102810 | 73.262 | 187 | 32 | 2 | 17 | 203 | 14 | 182 | 1.04e-92 | 269 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005556.01.T01 | AT3G22840 | 49.761 | 209 | 85 | 4 | 1 | 203 | 1 | 195 | 9.71e-61 | 188 |
| MsG0180005556.01.T01 | AT4G14690 | 53.431 | 204 | 83 | 4 | 1 | 203 | 1 | 193 | 1.07e-59 | 186 |
Find 57 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCAATGAGCACCATTTC+TGG | 0.210857 | 1:+93239267 | MsG0180005556.01.T01:CDS |
| TGGTGGCATCCCTTGGTTCT+TGG | 0.234840 | 1:+93239879 | MsG0180005556.01.T01:CDS |
| GGTGGCATCCCTTGGTTCTT+GGG | 0.263333 | 1:+93239880 | MsG0180005556.01.T01:CDS |
| GGTTTGTTTGATCAAATATA+TGG | 0.294584 | 1:+93239856 | MsG0180005556.01.T01:CDS |
| GGTGATGATGATGGTGTAAT+TGG | 0.299898 | 1:-93239540 | None:intergenic |
| GGTTGTGATGGTGGTGGTGG+TGG | 0.330653 | 1:-93239576 | None:intergenic |
| CAGAAGGGATGTTGCTGAAT+TGG | 0.358772 | 1:-93239305 | None:intergenic |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1:+93240020 | MsG0180005556.01.T01:CDS |
| GGTGGTGGTGGTGTTGGTGT+TGG | 0.372377 | 1:-93239561 | None:intergenic |
| AATGGAAGGTTGGCTATGAT+TGG | 0.393839 | 1:+93239793 | MsG0180005556.01.T01:CDS |
| GGCCTTTACAGAGTATGTTA+AGG | 0.395379 | 1:+93240041 | MsG0180005556.01.T01:CDS |
| GCTGGTTGTGATGGTGGTGG+TGG | 0.398279 | 1:-93239579 | None:intergenic |
| GCCTTTACAGAGTATGTTAA+GGG | 0.401917 | 1:+93240042 | MsG0180005556.01.T01:CDS |
| CCATTGATTCTCTCAGGTGC+TGG | 0.411549 | 1:-93239775 | None:intergenic |
| AACCTTCCATTGATTCTCTC+AGG | 0.436061 | 1:-93239781 | None:intergenic |
| GACTCTAGACTTGCCAGAAA+TGG | 0.452328 | 1:-93239280 | None:intergenic |
| GTGGCATCCCTTGGTTCTTG+GGG | 0.453269 | 1:+93239881 | MsG0180005556.01.T01:CDS |
| TGCCATCAAGTCTGAAAACT+TGG | 0.455106 | 1:-93239743 | None:intergenic |
| GAATGGAAGAATTGCTATGT+TGG | 0.460944 | 1:+93240005 | MsG0180005556.01.T01:CDS |
| GTGATGATGATGGTGTAATT+GGG | 0.461935 | 1:-93239539 | None:intergenic |
| TACCTTTGGTGCGCGAGTAT+AGG | 0.465801 | 1:-93239601 | None:intergenic |
| TCAGACTTGATGGCATTTGG+TGG | 0.470606 | 1:+93239751 | MsG0180005556.01.T01:CDS |
| GTTGGTGTTGGTGATGATGA+TGG | 0.479914 | 1:-93239549 | None:intergenic |
| CAGACTTGATGGCATTTGGT+GGG | 0.492385 | 1:+93239752 | MsG0180005556.01.T01:CDS |
| AATGGAAGAATTGCTATGTT+GGG | 0.499743 | 1:+93240006 | MsG0180005556.01.T01:CDS |
| CCAGCACCTGAGAGAATCAA+TGG | 0.516939 | 1:+93239775 | MsG0180005556.01.T01:CDS |
| AGTGTTGAGTCCAAGTCAAA+AGG | 0.522475 | 1:+93239955 | MsG0180005556.01.T01:CDS |
| TTTGGTGCGCGAGTATAGGC+TGG | 0.530750 | 1:-93239597 | None:intergenic |
| TAGGCTGGTTGTGATGGTGG+TGG | 0.536961 | 1:-93239582 | None:intergenic |
| TCAGATGCTGAGCTGTGGAA+TGG | 0.542277 | 1:+93239988 | MsG0180005556.01.T01:CDS |
| AACCTTAATTACCTCAGCCA+TGG | 0.545130 | 1:-93239373 | None:intergenic |
| GGTGGAGATAGCAAATGGAC+AGG | 0.547189 | 1:+93239834 | MsG0180005556.01.T01:CDS |
| ATAGCATTCCTTCTGAAGCT+AGG | 0.551512 | 1:-93239336 | None:intergenic |
| TATATGGTGGTGGCATCCCT+TGG | 0.552085 | 1:+93239872 | MsG0180005556.01.T01:CDS |
| ACCCTTAACATACTCTGTAA+AGG | 0.552111 | 1:-93240043 | None:intergenic |
| CGAGTATAGGCTGGTTGTGA+TGG | 0.554321 | 1:-93239588 | None:intergenic |
| TGAGAGAATCAATGGAAGGT+TGG | 0.560259 | 1:+93239783 | MsG0180005556.01.T01:CDS |
| GATGGTGTAATTGGGTCTAC+AGG | 0.568013 | 1:-93239531 | None:intergenic |
| TTTGATCAAATATATGGTGG+TGG | 0.569010 | 1:+93239862 | MsG0180005556.01.T01:CDS |
| AAGCTAGGCATGTACACAGA+AGG | 0.579754 | 1:-93239321 | None:intergenic |
| TGGACTCAACACTAACACCT+TGG | 0.588832 | 1:-93239945 | None:intergenic |
| AGCCTATACTCGCGCACCAA+AGG | 0.589710 | 1:+93239599 | MsG0180005556.01.T01:CDS |
| TTGTTTGATCAAATATATGG+TGG | 0.590396 | 1:+93239859 | MsG0180005556.01.T01:CDS |
| TGTCCTCAGATGCTGAGCTG+TGG | 0.594735 | 1:+93239983 | MsG0180005556.01.T01:CDS |
| CACTTGTCCCCAAGAACCAA+GGG | 0.598517 | 1:-93239888 | None:intergenic |
| TATCTTGAAAGTTCGATCCA+TGG | 0.599815 | 1:+93239356 | MsG0180005556.01.T01:CDS |
| GTATAGGCTGGTTGTGATGG+TGG | 0.608859 | 1:-93239585 | None:intergenic |
| AGCTAGGCATGTACACAGAA+GGG | 0.614155 | 1:-93239320 | None:intergenic |
| ATTCCACAGCTCAGCATCTG+AGG | 0.616858 | 1:-93239986 | None:intergenic |
| GAAAGTTCGATCCATGGCTG+AGG | 0.622095 | 1:+93239362 | MsG0180005556.01.T01:CDS |
| TGTACATGCCTAGCTTCAGA+AGG | 0.622254 | 1:+93239328 | MsG0180005556.01.T01:CDS |
| ACACTTGTCCCCAAGAACCA+AGG | 0.642060 | 1:-93239889 | None:intergenic |
| GTGGAGATAGCAAATGGACA+GGG | 0.645295 | 1:+93239835 | MsG0180005556.01.T01:CDS |
| CACCTGAGAGAATCAATGGA+AGG | 0.646646 | 1:+93239779 | MsG0180005556.01.T01:CDS |
| TCCCTATAGAAATCTACACA+AGG | 0.653374 | 1:-93240071 | None:intergenic |
| CCCTATAGAAATCTACACAA+GGG | 0.666479 | 1:-93240070 | None:intergenic |
| TATAGAAATCTACACAAGGG+AGG | 0.702658 | 1:-93240067 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAGATAAACTAAATTTA+AGG | + | Chr1:93239394-93239413 | MsG0180005556.01.T01:intron | 10.0% |
| !!! | TTTATGTATAGTATTGATTT+TGG | + | Chr1:93239662-93239681 | MsG0180005556.01.T01:intron | 15.0% |
| !! | ACATAATATGTTTATGATTC+AGG | + | Chr1:93239482-93239501 | MsG0180005556.01.T01:intron | 20.0% |
| !! | GAATCATAAACATATTATGT+TGG | - | Chr1:93239482-93239501 | None:intergenic | 20.0% |
| ! | AAAACAATTATGCTTACCTT+TGG | - | Chr1:93239618-93239637 | None:intergenic | 25.0% |
| ! | ATCGTCATTATTCAATGAAA+GGG | - | Chr1:93239703-93239722 | None:intergenic | 25.0% |
| ! | TATCGTCATTATTCAATGAA+AGG | - | Chr1:93239704-93239723 | None:intergenic | 25.0% |
| ! | TTGTTTGATCAAATATATGG+TGG | + | Chr1:93239859-93239878 | MsG0180005556.01.T01:CDS | 25.0% |
| !! | GGTTTGTTTGATCAAATATA+TGG | + | Chr1:93239856-93239875 | MsG0180005556.01.T01:CDS | 25.0% |
| AATGGAAGAATTGCTATGTT+GGG | + | Chr1:93240006-93240025 | MsG0180005556.01.T01:CDS | 30.0% | |
| TTTGATCAAATATATGGTGG+TGG | + | Chr1:93239862-93239881 | MsG0180005556.01.T01:CDS | 30.0% | |
| !! | TCTTTGATTCCATTTTTCCA+AGG | + | Chr1:93239928-93239947 | MsG0180005556.01.T01:CDS | 30.0% |
| ACACTAACACCTTGGAAAAA+TGG | - | Chr1:93239940-93239959 | None:intergenic | 35.0% | |
| ACCCTTAACATACTCTGTAA+AGG | - | Chr1:93240046-93240065 | None:intergenic | 35.0% | |
| GAATGGAAGAATTGCTATGT+TGG | + | Chr1:93240005-93240024 | MsG0180005556.01.T01:CDS | 35.0% | |
| GCCTTTACAGAGTATGTTAA+GGG | + | Chr1:93240042-93240061 | MsG0180005556.01.T01:CDS | 35.0% | |
| TATCTTGAAAGTTCGATCCA+TGG | + | Chr1:93239356-93239375 | MsG0180005556.01.T01:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | Chr1:93240020-93240039 | MsG0180005556.01.T01:CDS | 35.0% | |
| TGAGCAAAAAGAGCAATCAA+AGG | + | Chr1:93239506-93239525 | MsG0180005556.01.T01:CDS | 35.0% | |
| ! | GGTGATGATGATTGTGTAAT+CGG | - | Chr1:93239461-93239480 | None:intergenic | 35.0% |
| !! | GTGATGATGATGGTGTAATT+GGG | - | Chr1:93239542-93239561 | None:intergenic | 35.0% |
| !! | TTTTCAGACTTGATGGCATT+TGG | + | Chr1:93239748-93239767 | MsG0180005556.01.T01:CDS | 35.0% |
| !!! | ATTGGTTTTGTAGCAGCAAT+GGG | + | Chr1:93239811-93239830 | MsG0180005556.01.T01:CDS | 35.0% |
| AACCTTAATTACCTCAGCCA+TGG | - | Chr1:93239376-93239395 | None:intergenic | 40.0% | |
| ATCCATGGCTGAGGTAATTA+AGG | + | Chr1:93239371-93239390 | MsG0180005556.01.T01:intron | 40.0% | |
| GGCCTTTACAGAGTATGTTA+AGG | + | Chr1:93240041-93240060 | MsG0180005556.01.T01:CDS | 40.0% | |
| TGAGAGAATCAATGGAAGGT+TGG | + | Chr1:93239783-93239802 | MsG0180005556.01.T01:CDS | 40.0% | |
| TGCCATCAAGTCTGAAAACT+TGG | - | Chr1:93239746-93239765 | None:intergenic | 40.0% | |
| ! | AATGGAAGGTTGGCTATGAT+TGG | + | Chr1:93239793-93239812 | MsG0180005556.01.T01:CDS | 40.0% |
| ! | AGTGTTGAGTCCAAGTCAAA+AGG | + | Chr1:93239955-93239974 | MsG0180005556.01.T01:CDS | 40.0% |
| ! | CACCAAGTTTTCAGACTTGA+TGG | + | Chr1:93239741-93239760 | MsG0180005556.01.T01:CDS | 40.0% |
| !! | AACCTTCCATTGATTCTCTC+AGG | - | Chr1:93239784-93239803 | None:intergenic | 40.0% |
| !! | ATAGCATTCCTTCTGAAGCT+AGG | - | Chr1:93239339-93239358 | None:intergenic | 40.0% |
| !! | GGTGATGATGATGGTGTAAT+TGG | - | Chr1:93239543-93239562 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCAGCAA+TGG | + | Chr1:93239810-93239829 | MsG0180005556.01.T01:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCAGCAATG+GGG | + | Chr1:93239812-93239831 | MsG0180005556.01.T01:CDS | 40.0% |
| AAGCTAGGCATGTACACAGA+AGG | - | Chr1:93239324-93239343 | None:intergenic | 45.0% | |
| AGCTAGGCATGTACACAGAA+GGG | - | Chr1:93239323-93239342 | None:intergenic | 45.0% | |
| AGCTCAATGAGCACCATTTC+TGG | + | Chr1:93239267-93239286 | MsG0180005556.01.T01:CDS | 45.0% | |
| CACCTGAGAGAATCAATGGA+AGG | + | Chr1:93239779-93239798 | MsG0180005556.01.T01:CDS | 45.0% | |
| CAGAAGGGATGTTGCTGAAT+TGG | - | Chr1:93239308-93239327 | None:intergenic | 45.0% | |
| GACTCTAGACTTGCCAGAAA+TGG | - | Chr1:93239283-93239302 | None:intergenic | 45.0% | |
| GATGGTGTAATTGGGTCTAC+AGG | - | Chr1:93239534-93239553 | None:intergenic | 45.0% | |
| GTGGAGATAGCAAATGGACA+GGG | + | Chr1:93239835-93239854 | MsG0180005556.01.T01:CDS | 45.0% | |
| TGGACTCAACACTAACACCT+TGG | - | Chr1:93239948-93239967 | None:intergenic | 45.0% | |
| TGTACATGCCTAGCTTCAGA+AGG | + | Chr1:93239328-93239347 | MsG0180005556.01.T01:CDS | 45.0% | |
| ! | CAGACTTGATGGCATTTGGT+GGG | + | Chr1:93239752-93239771 | MsG0180005556.01.T01:CDS | 45.0% |
| ! | TCAGACTTGATGGCATTTGG+TGG | + | Chr1:93239751-93239770 | MsG0180005556.01.T01:CDS | 45.0% |
| !! | GGACATGACACCTTTTGACT+TGG | - | Chr1:93239968-93239987 | None:intergenic | 45.0% |
| !! | GTTGGTGTTGGTGATGATGA+TGG | - | Chr1:93239552-93239571 | None:intergenic | 45.0% |
| !!! | TGGTTTTGTAGCAGCAATGG+GGG | + | Chr1:93239813-93239832 | MsG0180005556.01.T01:CDS | 45.0% |
| ACACTTGTCCCCAAGAACCA+AGG | - | Chr1:93239892-93239911 | None:intergenic | 50.0% | |
| ATGGGGGTGGAGATAGCAAA+TGG | + | Chr1:93239829-93239848 | MsG0180005556.01.T01:CDS | 50.0% | |
| ATTCCACAGCTCAGCATCTG+AGG | - | Chr1:93239989-93240008 | None:intergenic | 50.0% | |
| CACTTGTCCCCAAGAACCAA+GGG | - | Chr1:93239891-93239910 | None:intergenic | 50.0% | |
| CCAGCACCTGAGAGAATCAA+TGG | + | Chr1:93239775-93239794 | MsG0180005556.01.T01:CDS | 50.0% | |
| GAAAGTTCGATCCATGGCTG+AGG | + | Chr1:93239362-93239381 | MsG0180005556.01.T01:CDS | 50.0% | |
| GGTGGAGATAGCAAATGGAC+AGG | + | Chr1:93239834-93239853 | MsG0180005556.01.T01:CDS | 50.0% | |
| TCAGATGCTGAGCTGTGGAA+TGG | + | Chr1:93239988-93240007 | MsG0180005556.01.T01:CDS | 50.0% | |
| ! | CGAGTATAGGCTGGTTGTGA+TGG | - | Chr1:93239591-93239610 | None:intergenic | 50.0% |
| ! | GTATAGGCTGGTTGTGATGG+TGG | - | Chr1:93239588-93239607 | None:intergenic | 50.0% |
| ! | TACCTTTGGTGCGCGAGTAT+AGG | - | Chr1:93239604-93239623 | None:intergenic | 50.0% |
| ! | TTTTGTAGCAGCAATGGGGG+TGG | + | Chr1:93239816-93239835 | MsG0180005556.01.T01:CDS | 50.0% |
| !! | CCATTGATTCTCTCAGGTGC+TGG | - | Chr1:93239778-93239797 | None:intergenic | 50.0% |
| !! | TATATGGTGGTGGCATCCCT+TGG | + | Chr1:93239872-93239891 | MsG0180005556.01.T01:CDS | 50.0% |
| AGCCTATACTCGCGCACCAA+AGG | + | Chr1:93239599-93239618 | MsG0180005556.01.T01:CDS | 55.0% | |
| TGTCCTCAGATGCTGAGCTG+TGG | + | Chr1:93239983-93240002 | MsG0180005556.01.T01:CDS | 55.0% | |
| ! | GGTGGCATCCCTTGGTTCTT+GGG | + | Chr1:93239880-93239899 | MsG0180005556.01.T01:CDS | 55.0% |
| ! | GTGGCATCCCTTGGTTCTTG+GGG | + | Chr1:93239881-93239900 | MsG0180005556.01.T01:CDS | 55.0% |
| ! | TGGTGGCATCCCTTGGTTCT+TGG | + | Chr1:93239879-93239898 | MsG0180005556.01.T01:CDS | 55.0% |
| ! | TTTGGTGCGCGAGTATAGGC+TGG | - | Chr1:93239600-93239619 | None:intergenic | 55.0% |
| !! | TAGGCTGGTTGTGATGGTGG+TGG | - | Chr1:93239585-93239604 | None:intergenic | 55.0% |
| !! | GCTGGTTGTGATGGTGGTGG+TGG | - | Chr1:93239582-93239601 | None:intergenic | 60.0% |
| !! | GGTGGTGGTGGTGTTGGTGT+TGG | - | Chr1:93239564-93239583 | None:intergenic | 60.0% |
| !! | GGTTGTGATGGTGGTGGTGG+TGG | - | Chr1:93239579-93239598 | None:intergenic | 60.0% |
| !! | TGTGATGGTGGTGGTGGTGG+TGG | - | Chr1:93239576-93239595 | None:intergenic | 60.0% |
| !! | GGTGGTGGTGGTGGTGGTGT+TGG | - | Chr1:93239570-93239589 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 93239234 | 93240080 | 93239234 | ID=MsG0180005556.01;Name=MsG0180005556.01 |
| Chr1 | mRNA | 93239234 | 93240080 | 93239234 | ID=MsG0180005556.01.T01;Parent=MsG0180005556.01;Name=MsG0180005556.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.66|3|0|203 |
| Chr1 | exon | 93239234 | 93239383 | 93239234 | ID=MsG0180005556.01.T01:exon:16065;Parent=MsG0180005556.01.T01 |
| Chr1 | exon | 93239504 | 93239620 | 93239504 | ID=MsG0180005556.01.T01:exon:16066;Parent=MsG0180005556.01.T01 |
| Chr1 | exon | 93239736 | 93240080 | 93239736 | ID=MsG0180005556.01.T01:exon:16067;Parent=MsG0180005556.01.T01 |
| Chr1 | CDS | 93239234 | 93239383 | 93239234 | ID=MsG0180005556.01.T01:cds;Parent=MsG0180005556.01.T01 |
| Chr1 | CDS | 93239504 | 93239620 | 93239504 | ID=MsG0180005556.01.T01:cds;Parent=MsG0180005556.01.T01 |
| Chr1 | CDS | 93239736 | 93240080 | 93239736 | ID=MsG0180005556.01.T01:cds;Parent=MsG0180005556.01.T01 |
| Gene Sequence |
| Protein sequence |
