AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032832.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032832.01.T01 MTR_6g053090 98.710 155 2 0 1 155 78 232 2.22e-111 320
MsG0680032832.01.T01 MTR_3g024400 48.592 142 73 0 14 155 75 216 5.23e-41 138
MsG0680032832.01.T01 MTR_5g012120 47.706 109 57 0 39 147 4 112 1.40e-30 109
MsG0680032832.01.T01 MTR_5g012120 47.706 109 57 0 39 147 81 189 7.49e-30 109
MsG0680032832.01.T01 MTR_3g024400 50.685 73 36 0 83 155 96 168 8.80e-19 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032832.01.T01 AT4G21190 72.258 155 43 0 1 155 31 185 1.68e-81 243
MsG0680032832.01.T01 AT4G18975 50.704 142 70 0 14 155 57 198 6.20e-47 153
MsG0680032832.01.T01 AT4G18975 50.704 142 70 0 14 155 84 225 1.24e-46 153
MsG0680032832.01.T01 AT4G18975 50.704 142 70 0 14 155 84 225 1.24e-46 153
MsG0680032832.01.T01 AT4G18975 50.704 142 70 0 14 155 84 225 1.24e-46 153
MsG0680032832.01.T01 AT1G04590 40.559 143 85 0 13 155 131 273 7.17e-31 114
MsG0680032832.01.T01 AT1G04590 42.222 135 78 0 13 147 131 265 1.91e-30 113

Find 25 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATAATTACAGTGAAAA+AGG 0.241597 6:-50627430 MsG0680032832.01.T01:CDS
GATGTCAAAGAAGAAGTTTA+TGG 0.321806 6:-50627489 MsG0680032832.01.T01:CDS
TGAAACTATAGGTAACAAAA+TGG 0.351629 6:-50627145 MsG0680032832.01.T01:intron
TTGACGAAGCAGAAGAGCTT+TGG 0.377250 6:-50627046 MsG0680032832.01.T01:CDS
GACCAAGATATCCTCGTGTT+TGG 0.399250 6:-50628814 MsG0680032832.01.T01:CDS
TTCCAAACACGAGGATATCT+TGG 0.413701 6:+50628812 None:intergenic
CTCTTGATACCTATGTTGCT+TGG 0.421878 6:-50627464 MsG0680032832.01.T01:CDS
AAGGTATTTGCTGCTGCAAA+AGG 0.444833 6:-50628842 MsG0680032832.01.T01:CDS
TCTTGATACCTATGTTGCTT+GGG 0.447214 6:-50627463 MsG0680032832.01.T01:CDS
CTCTAGAATACGAGAAAGAA+TGG 0.450096 6:-50627398 MsG0680032832.01.T01:CDS
ATATCCATCTACTGTAAAAG+AGG 0.495318 6:-50626966 MsG0680032832.01.T01:CDS
CTTGCATGATAAGATGTTTG+AGG 0.498325 6:-50626943 MsG0680032832.01.T01:CDS
AACAAAATGGATGTTAAGCA+AGG 0.507980 6:-50627132 MsG0680032832.01.T01:CDS
ACACGAGGATATCTTGGTCT+TGG 0.514375 6:+50628818 None:intergenic
GCTCTTCTGCTTCGTCAACT+CGG 0.545541 6:+50627051 None:intergenic
TTCTAGTTCCCAAGCAACAT+AGG 0.547094 6:+50627455 None:intergenic
AATACGAGAAAGAATGGAAG+CGG 0.559112 6:-50627392 MsG0680032832.01.T01:CDS
AAGGGTCAAGGAAAGACAAT+GGG 0.563191 6:-50627113 MsG0680032832.01.T01:CDS
AGAATGGAAGCGGATAATAC+AGG 0.564642 6:-50627382 MsG0680032832.01.T01:intron
ATTACTTAATGCTTTAGCAG+AGG 0.581952 6:-50627078 MsG0680032832.01.T01:CDS
TGGATGTTAAGCAAGGGTCA+AGG 0.625921 6:-50627125 MsG0680032832.01.T01:CDS
ACAAAATGGATGTTAAGCAA+GGG 0.632530 6:-50627131 MsG0680032832.01.T01:CDS
CAAGGGTCAAGGAAAGACAA+TGG 0.634494 6:-50627114 MsG0680032832.01.T01:CDS
TTATTAGTCTTCCAAACACG+AGG 0.649651 6:+50628803 None:intergenic
AAAGCTATTAATGCAGTACA+CGG 0.681216 6:-50627021 MsG0680032832.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAGAAAATCACATAATT+CGG - Chr6:50627295-50627314 MsG0680032832.01.T01:intron 15.0%
!!! TGATTTTCTAAATTTATTAG+CGG + Chr6:50627288-50627307 None:intergenic 15.0%
!! AAAGAAGGTAAAAAATCATA+AGG + Chr6:50628443-50628462 None:intergenic 20.0%
!! GGAAGACTAATAAAAAAATT+GGG - Chr6:50626981-50627000 MsG0680032832.01.T01:CDS 20.0%
!! TGGAAGACTAATAAAAAAAT+TGG - Chr6:50626980-50626999 MsG0680032832.01.T01:CDS 20.0%
!!! AAATTACTTTCTGAAACTAT+AGG - Chr6:50628618-50628637 MsG0680032832.01.T01:intron 20.0%
! TCCTATAATTACAGTGAAAA+AGG - Chr6:50628344-50628363 MsG0680032832.01.T01:intron 25.0%
! TGAAACTATAGGTAACAAAA+TGG - Chr6:50628629-50628648 MsG0680032832.01.T01:intron 25.0%
!! ATTTTATCAAAGAATCTACG+TGG + Chr6:50628787-50628806 None:intergenic 25.0%
!! GATTTTATACAGAAAACCTT+AGG - Chr6:50628186-50628205 MsG0680032832.01.T01:intron 25.0%
!!! AAAAATTGGGACTATTTCTA+AGG - Chr6:50626994-50627013 MsG0680032832.01.T01:CDS 25.0%
!!! ACCTTTTTCACTGTAATTAT+AGG + Chr6:50628348-50628367 None:intergenic 25.0%
AAACAAATACAGAGTATGCA+AGG + Chr6:50627050-50627069 None:intergenic 30.0%
AAAGCTATTAATGCAGTACA+CGG - Chr6:50628753-50628772 MsG0680032832.01.T01:intron 30.0%
AACAAAATGGATGTTAAGCA+AGG - Chr6:50628642-50628661 MsG0680032832.01.T01:intron 30.0%
AATTTCAAAAAGCTACCACA+TGG - Chr6:50628032-50628051 MsG0680032832.01.T01:intron 30.0%
ACAAAATGGATGTTAAGCAA+GGG - Chr6:50628643-50628662 MsG0680032832.01.T01:intron 30.0%
ATATCCATCTACTGTAAAAG+AGG - Chr6:50628808-50628827 MsG0680032832.01.T01:CDS 30.0%
ATTACTTAATGCTTTAGCAG+AGG - Chr6:50628696-50628715 MsG0680032832.01.T01:intron 30.0%
GATGTCAAAGAAGAAGTTTA+TGG - Chr6:50628285-50628304 MsG0680032832.01.T01:intron 30.0%
GCACATACATTTAACAGATT+AGG + Chr6:50627385-50627404 None:intergenic 30.0%
TCTATGGAAAAGCTACTTAT+TGG - Chr6:50627796-50627815 MsG0680032832.01.T01:intron 30.0%
! CTCTTAATGGCTAATCTTTT+GGG - Chr6:50628127-50628146 MsG0680032832.01.T01:intron 30.0%
! TATTTTATGGCGGTTCAAAA+TGG - Chr6:50627933-50627952 MsG0680032832.01.T01:intron 30.0%
! TTTGTCTATGAACTAGTCTA+TGG - Chr6:50627780-50627799 MsG0680032832.01.T01:intron 30.0%
!! ATGAGAGTATTATGTCGTAT+TGG - Chr6:50627483-50627502 MsG0680032832.01.T01:CDS 30.0%
!!! TGGTAGCTTTTTGAAATTTG+TGG + Chr6:50628030-50628049 None:intergenic 30.0%
AATACGAGAAAGAATGGAAG+CGG - Chr6:50628382-50628401 MsG0680032832.01.T01:intron 35.0%
ATTACCGGGTTGTCAAATAT+CGG - Chr6:50627833-50627852 MsG0680032832.01.T01:intron 35.0%
ATTCCTTTGATCAGAAGAGT+CGG - Chr6:50627538-50627557 MsG0680032832.01.T01:intron 35.0%
CCAAAACGTATAAACTACCA+AGG + Chr6:50627578-50627597 None:intergenic 35.0%
CCAGAAATGTAAATCATCCA+AGG + Chr6:50628221-50628240 None:intergenic 35.0%
CCTTGGATGATTTACATTTC+TGG - Chr6:50628218-50628237 MsG0680032832.01.T01:intron 35.0%
CTATAGTGCTGCAATAAAGA+AGG + Chr6:50628458-50628477 None:intergenic 35.0%
CTGGATTAAGATACATTACC+GGG - Chr6:50627819-50627838 MsG0680032832.01.T01:intron 35.0%
CTGGTAGTTATTCGAGAAAA+CGG - Chr6:50627451-50627470 MsG0680032832.01.T01:CDS 35.0%
CTTGCATGATAAGATGTTTG+AGG - Chr6:50628831-50628850 MsG0680032832.01.T01:CDS 35.0%
GATGCAGTGACTTCTTTATA+TGG + Chr6:50627726-50627745 None:intergenic 35.0%
GCTGGATTAAGATACATTAC+CGG - Chr6:50627818-50627837 MsG0680032832.01.T01:intron 35.0%
! AAAGATTAGCCATTAAGAGC+CGG + Chr6:50628126-50628145 None:intergenic 35.0%
! GCACTAACCCTTTTTATCAA+AGG + Chr6:50627184-50627203 None:intergenic 35.0%
! GCTCTTAATGGCTAATCTTT+TGG - Chr6:50628126-50628145 MsG0680032832.01.T01:intron 35.0%
! GCTGATTTATGTTCCTTCTT+TGG - Chr6:50628566-50628585 MsG0680032832.01.T01:intron 35.0%
! TCTTGATACCTATGTTGCTT+GGG - Chr6:50628311-50628330 MsG0680032832.01.T01:intron 35.0%
! TTCGGTGTAATTATATGCGT+TGG - Chr6:50627313-50627332 MsG0680032832.01.T01:intron 35.0%
!! ACAATGTTGTTGATAGCGAA+TGG - Chr6:50627682-50627701 MsG0680032832.01.T01:intron 35.0%
!! CCTTGGTAGTTTATACGTTT+TGG - Chr6:50627575-50627594 MsG0680032832.01.T01:intron 35.0%
!! CTCTAGAATACGAGAAAGAA+TGG - Chr6:50628376-50628395 MsG0680032832.01.T01:intron 35.0%
!! TAATGGCTAATCTTTTGGGT+TGG - Chr6:50628131-50628150 MsG0680032832.01.T01:intron 35.0%
!! TTATTAGTCTTCCAAACACG+AGG + Chr6:50626974-50626993 None:intergenic 35.0%
!!! CACCATGGCGTTTTATTTTA+TGG - Chr6:50627920-50627939 MsG0680032832.01.T01:intron 35.0%
!!! CATGGCGTTTTATTTTATGG+CGG - Chr6:50627923-50627942 MsG0680032832.01.T01:intron 35.0%
AAAATAAAACGCCATGGTGC+TGG + Chr6:50627919-50627938 None:intergenic 40.0%
AAAGAAGTCACTGCATCCTT+TGG - Chr6:50627729-50627748 MsG0680032832.01.T01:intron 40.0%
AAGGGTCAAGGAAAGACAAT+GGG - Chr6:50628661-50628680 MsG0680032832.01.T01:intron 40.0%
ACATAGACACCTATGAAGCA+TGG - Chr6:50627215-50627234 MsG0680032832.01.T01:intron 40.0%
ACTACCACATCACAACTAGT+GGG + Chr6:50627604-50627623 None:intergenic 40.0%
AGAATGGAAGCGGATAATAC+AGG - Chr6:50628392-50628411 MsG0680032832.01.T01:intron 40.0%
ATGAAGCATGGATACAGACA+CGG - Chr6:50627227-50627246 MsG0680032832.01.T01:intron 40.0%
ATGGATGTCGTCTCTTAAAG+AGG - Chr6:50628078-50628097 MsG0680032832.01.T01:intron 40.0%
CACTGAAACATGTTAGACAC+TGG - Chr6:50627513-50627532 MsG0680032832.01.T01:intron 40.0%
CGCCATAAAATAAAACGCCA+TGG + Chr6:50627925-50627944 None:intergenic 40.0%
CTCTTGATACCTATGTTGCT+TGG - Chr6:50628310-50628329 MsG0680032832.01.T01:intron 40.0%
GGACACGCCTTTGATAAAAA+GGG - Chr6:50627174-50627193 MsG0680032832.01.T01:intron 40.0%
TACTACCACATCACAACTAG+TGG + Chr6:50627605-50627624 None:intergenic 40.0%
TCCAAGGAAAAATCAGCCTA+AGG + Chr6:50628205-50628224 None:intergenic 40.0%
TGCTGATACAAAAGCTACCA+TGG - Chr6:50628059-50628078 MsG0680032832.01.T01:intron 40.0%
TGGAAAAGCTACTTATTGGC+TGG - Chr6:50627800-50627819 MsG0680032832.01.T01:intron 40.0%
TGTCTGTATCCATGCTTCAT+AGG + Chr6:50627227-50627246 None:intergenic 40.0%
TGTGGTAGTAGTACTCTTTG+TGG - Chr6:50627615-50627634 MsG0680032832.01.T01:intron 40.0%
TTCCAAACACGAGGATATCT+TGG + Chr6:50626965-50626984 None:intergenic 40.0%
TTTATGGCGGTTCAAAATGG+TGG - Chr6:50627936-50627955 MsG0680032832.01.T01:intron 40.0%
! ACATCATTGTCGACTGCAAA+TGG + Chr6:50627977-50627996 None:intergenic 40.0%
! CAAGCCTCTTTTACAGTAGA+TGG + Chr6:50628815-50628834 None:intergenic 40.0%
! GATCTGAAGCTATCCAAAGA+AGG + Chr6:50628582-50628601 None:intergenic 40.0%
! GTCAATGTGAGTGTTGTATC+TGG + Chr6:50627115-50627134 None:intergenic 40.0%
! TCTTTAAGAGACGACATCCA+TGG + Chr6:50628079-50628098 None:intergenic 40.0%
!! ACCTTAGGCTGATTTTTCCT+TGG - Chr6:50628201-50628220 MsG0680032832.01.T01:intron 40.0%
!! CTTTTTATCAAAGGCGTGTC+CGG + Chr6:50627175-50627194 None:intergenic 40.0%
!! TTCTAGTTCCCAAGCAACAT+AGG + Chr6:50628322-50628341 None:intergenic 40.0%
ACACAACACGTGTCCAATAC+CGG - Chr6:50627353-50627372 MsG0680032832.01.T01:intron 45.0%
ACACGAGGATATCTTGGTCT+TGG + Chr6:50626959-50626978 None:intergenic 45.0%
ATGGCCGATATTTGACAACC+CGG + Chr6:50627840-50627859 None:intergenic 45.0%
CAAGGGTCAAGGAAAGACAA+TGG - Chr6:50628660-50628679 MsG0680032832.01.T01:intron 45.0%
CAATGTTATGAGTTCCACGC+CGG - Chr6:50628104-50628123 MsG0680032832.01.T01:intron 45.0%
CATGTGAGACGATGTTGTGT+TGG - Chr6:50627134-50627153 MsG0680032832.01.T01:CDS 45.0%
CCAGCATGAGAAACTTGTGA+CGG - Chr6:50627890-50627909 MsG0680032832.01.T01:intron 45.0%
CGACATTGATACGTTGACAC+TGG - Chr6:50627432-50627451 MsG0680032832.01.T01:CDS 45.0%
CGGACACGCCTTTGATAAAA+AGG - Chr6:50627173-50627192 MsG0680032832.01.T01:intron 45.0%
GACCAAGATATCCTCGTGTT+TGG - Chr6:50626960-50626979 MsG0680032832.01.T01:CDS 45.0%
GATGTCGTCTCTTAAAGAGG+TGG - Chr6:50628081-50628100 MsG0680032832.01.T01:intron 45.0%
GTTTCCCACTAGTTGTGATG+TGG - Chr6:50627597-50627616 MsG0680032832.01.T01:intron 45.0%
TGGATGTTAAGCAAGGGTCA+AGG - Chr6:50628649-50628668 MsG0680032832.01.T01:intron 45.0%
TTAACAGATTAGGCGTGTCC+CGG + Chr6:50627375-50627394 None:intergenic 45.0%
TTGACGAAGCAGAAGAGCTT+TGG - Chr6:50628728-50628747 MsG0680032832.01.T01:intron 45.0%
TTGGTACATGTGTCGAACAC+CGG - Chr6:50627153-50627172 MsG0680032832.01.T01:intron 45.0%
TTGTCAAATATCGGCCATGC+TGG - Chr6:50627842-50627861 MsG0680032832.01.T01:intron 45.0%
! TTTTCTGCTGTCTGCGATTG+AGG - Chr6:50628539-50628558 MsG0680032832.01.T01:intron 45.0%
!! CGGTGCTATATCGTTAACCT+TGG - Chr6:50627558-50627577 MsG0680032832.01.T01:intron 45.0%
!! TGTTGATAGCGAATGGCAGA+TGG - Chr6:50627689-50627708 MsG0680032832.01.T01:intron 45.0%
!! TTGTATCAGCACTGACCATG+TGG + Chr6:50628050-50628069 None:intergenic 45.0%
AAGTCACTGCATCCTTTGGC+GGG - Chr6:50627733-50627752 MsG0680032832.01.T01:intron 50.0%
CACAACACGTGTCCAATACC+GGG - Chr6:50627354-50627373 MsG0680032832.01.T01:intron 50.0%
CACGGACACCAAACATGACA+CGG - Chr6:50627245-50627264 MsG0680032832.01.T01:intron 50.0%
CCGTCACAAGTTTCTCATGC+TGG + Chr6:50627893-50627912 None:intergenic 50.0%
GAAGTCACTGCATCCTTTGG+CGG - Chr6:50627732-50627751 MsG0680032832.01.T01:intron 50.0%
GCTCTTCTGCTTCGTCAACT+CGG + Chr6:50628726-50628745 None:intergenic 50.0%
GTCAGTGTCCGTGTCATGTT+TGG + Chr6:50627256-50627275 None:intergenic 50.0%
GTCGACTGCAAATGGCAGAA+AGG + Chr6:50627969-50627988 None:intergenic 50.0%
TAGTGAAGAGATGTCCGCCA+TGG + Chr6:50627760-50627779 None:intergenic 50.0%
TTCACCATCGAGATCCAGCA+TGG + Chr6:50627859-50627878 None:intergenic 50.0%
!! CGTTGGTGTTGTGTCGATGT+TGG - Chr6:50627330-50627349 MsG0680032832.01.T01:intron 50.0%
!! GCACCGACTCTTCTGATCAA+AGG + Chr6:50627544-50627563 None:intergenic 50.0%
!! TTGGGTTGGACTCTCTTCGT+GGG - Chr6:50628145-50628164 MsG0680032832.01.T01:intron 50.0%
!! TTTGGGTTGGACTCTCTTCG+TGG - Chr6:50628144-50628163 MsG0680032832.01.T01:intron 50.0%
GATTAGGCGTGTCCCGGTAT+TGG + Chr6:50627369-50627388 None:intergenic 55.0%
TTAGCCATTAAGAGCCGGCG+TGG + Chr6:50628121-50628140 None:intergenic 55.0%
!! AGTTCCACGCCGGCTCTTAA+TGG - Chr6:50628114-50628133 MsG0680032832.01.T01:intron 55.0%
ATCCTTTGGCGGGACCATGG+CGG - Chr6:50627743-50627762 MsG0680032832.01.T01:intron 60.0%
TCGGCCATGCTGGATCTCGA+TGG - Chr6:50627852-50627871 MsG0680032832.01.T01:intron 60.0%
TGCATCCTTTGGCGGGACCA+TGG - Chr6:50627740-50627759 MsG0680032832.01.T01:intron 60.0%
!! TGTGACGGATGCCAGCACCA+TGG - Chr6:50627905-50627924 MsG0680032832.01.T01:intron 60.0%
GTCCGCCATGGTCCCGCCAA+AGG + Chr6:50627748-50627767 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 50626935 50628861 50626935 ID=MsG0680032832.01;Name=MsG0680032832.01
Chr6 mRNA 50626935 50628861 50626935 ID=MsG0680032832.01.T01;Parent=MsG0680032832.01;Name=MsG0680032832.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|155
Chr6 exon 50628760 50628861 50628760 ID=MsG0680032832.01.T01:exon:10399;Parent=MsG0680032832.01.T01
Chr6 exon 50627383 50627526 50627383 ID=MsG0680032832.01.T01:exon:10400;Parent=MsG0680032832.01.T01
Chr6 exon 50626935 50627156 50626935 ID=MsG0680032832.01.T01:exon:10401;Parent=MsG0680032832.01.T01
Chr6 CDS 50628760 50628861 50628760 ID=MsG0680032832.01.T01:cds;Parent=MsG0680032832.01.T01
Chr6 CDS 50627383 50627526 50627383 ID=MsG0680032832.01.T01:cds;Parent=MsG0680032832.01.T01
Chr6 CDS 50626935 50627156 50626935 ID=MsG0680032832.01.T01:cds;Parent=MsG0680032832.01.T01
Gene Sequence

>MsG0680032832.01.T01

GTATTTGCTGCTGCAAAAGGTCCAAGACCAAGATATCCTCGTGTTTGGAAGACTAATAAAAAAATTGGGACTATTTCTAAGGCTGCTAAGCTTGTTCAATCTATTAAAGAACTTTCAGATGTCAAAGAAGAAGTTTATGGAGCTCTTGATACCTATGTTGCTTGGGAACTAGAATTTCCTATAATTACAGTGAAAAAGGTACTCAAGACTCTAGAATACGAGAAAGAATGGAAGCGGATAATACAGGTAACAAAATGGATGTTAAGCAAGGGTCAAGGAAAGACAATGGGAAGCTATTTTACATTACTTAATGCTTTAGCAGAGGATGACCGAGTTGACGAAGCAGAAGAGCTTTGGACAAAGCTATTAATGCAGTACACGGAAAGCTTGCCACGTAGATTCTTTGATAAAATGATATCCATCTACTGTAAAAGAGGCTTGCATGATAAGATGTTTGAGGTATTATGA

Protein sequence

>MsG0680032832.01.T01

VFAAAKGPRPRYPRVWKTNKKIGTISKAAKLVQSIKELSDVKEEVYGALDTYVAWELEFPIITVKKVLKTLEYEKEWKRIIQVTKWMLSKGQGKTMGSYFTLLNALAEDDRVDEAEELWTKLLMQYTESLPRRFFDKMISIYCKRGLHDKMFEVL*