AlfalfaGEDB | MsG0580025814.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025814.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025814.01.T01	XP_003613269.2	96.806	501	15	1	3	502	60	560	0	994

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000139.01	MsG0580025814.01	0.823712	1.195290e-53	1.037346e-50
MsG0180001356.01	MsG0580025814.01	0.805550	1.264485e-49	6.708885e-47
MsG0180001892.01	MsG0580025814.01	0.810945	8.955729e-51	5.472493e-48
MsG0180003094.01	MsG0580025814.01	0.821328	4.279968e-53	3.471449e-50
MsG0180003493.01	MsG0580025814.01	0.825123	5.565710e-54	5.027889e-51
MsG0180003651.01	MsG0580025814.01	0.844006	9.875051e-59	1.572909e-55
MsG0180003885.01	MsG0580025814.01	0.813331	2.701810e-51	1.759292e-48
MsG0180003951.01	MsG0580025814.01	0.800695	1.277325e-48	5.986373e-46
MsG0180004482.01	MsG0580025814.01	0.809618	1.731302e-50	1.021456e-47
MsG0180004769.01	MsG0580025814.01	0.816743	4.725891e-52	3.376677e-49

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PPI

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Gene1	Gene2	Type
MsG0880045026.01	MsG0580025814.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025814.01.T01	MTR_5g034750	96.806	501	15	1	3	502	60	560	0.0	994
MsG0580025814.01.T01	MTR_8g063760	67.976	509	106	7	2	502	62	521	0.0	643
MsG0580025814.01.T01	MTR_6g007720	58.889	360	129	6	7	347	64	423	1.16e-132	396
MsG0580025814.01.T01	MTR_2g075860	41.860	344	170	9	8	348	61	377	2.39e-72	238
MsG0580025814.01.T01	MTR_5g034550	66.250	160	29	3	61	206	1	149	1.54e-62	201
MsG0580025814.01.T01	MTR_8g058630	59.712	139	55	1	63	201	13	150	1.07e-47	162
MsG0580025814.01.T01	MTR_8g063700	37.313	201	119	4	13	209	31	228	4.03e-33	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025814.01.T01	AT1G70280	67.213	366	101	6	4	362	58	411	6.29e-168	484
MsG0580025814.01.T01	AT1G70280	67.313	361	99	6	9	362	1	349	4.09e-167	480
MsG0580025814.01.T01	AT1G23880	64.151	371	112	6	2	362	118	477	2.16e-157	459
MsG0580025814.01.T01	AT1G23880	64.543	361	113	6	9	362	1	353	9.04e-156	450
MsG0580025814.01.T01	AT5G14890	54.783	345	131	10	2	335	64	394	4.21e-112	340
MsG0580025814.01.T01	AT3G14860	43.731	327	160	7	8	331	69	374	3.72e-77	251
MsG0580025814.01.T01	AT3G14860	43.731	327	159	7	8	331	69	373	1.20e-76	249
MsG0580025814.01.T01	AT1G23890	48.315	178	85	5	4	179	19	191	1.34e-42	152
MsG0580025814.01.T01	AT1G23890	48.315	178	85	5	4	179	19	191	1.49e-41	153

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Find 125 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCTGTATAACGGAT+GGG	0.597862	5:+24595047	MsG0580025814.01.T01:CDS
AAAACATCTGTATAACGGAT+GGG	0.615653	5:+24594972	MsG0580025814.01.T01:CDS
AAAACATGTGTACAATGGAT+GGG	0.602015	5:+24594900	MsG0580025814.01.T01:CDS
AAAATGCAACAATTGTGGCA+AGG	0.653611	5:+24594820	MsG0580025814.01.T01:CDS
AAGGCAAGATCAAGAAATTT+TGG	0.177285	5:+24595501	MsG0580025814.01.T01:three_prime_UTR
AAGGTCTCTTTGCATCCATT+GGG	0.503206	5:+24594566	MsG0580025814.01.T01:CDS
AAGTACAATTGTCGCATCTC+AGG	0.448027	5:+24593905	MsG0580025814.01.T01:CDS
AATGAATCTCCAATAGGGGA+GGG	0.640999	5:+24595327	MsG0580025814.01.T01:three_prime_UTR
AATGTGGAAACTGTGTTTGA+TGG	0.487534	5:+24592601	MsG0580025814.01.T01:CDS
AATTGAGCCTTATGCAGTTG+AGG	0.593409	5:+24592636	MsG0580025814.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATGTGTACAATGGAT+GGG	+	Chr5:24594900-24594919	MsG0580025814.01.T01:CDS	30.0%
AAATAAATCGGATAGACAGT+TGG	+	Chr5:24594158-24594177	MsG0580025814.01.T01:intron	30.0%
AACTGTCTTGTAGAAATTCA+AGG	+	Chr5:24593105-24593124	MsG0580025814.01.T01:intron	30.0%
AAGCATTTGTAGGATTGTTT+GGG	+	Chr5:24593224-24593243	MsG0580025814.01.T01:intron	30.0%
AATAAGCTGAAAACAGCTTA+TGG	-	Chr5:24593271-24593290	None:intergenic	30.0%
ATAAGCTGAAAACAGCTTAT+GGG	-	Chr5:24593270-24593289	None:intergenic	30.0%
ATTACAAAACTTTCTTGCAC+AGG	-	Chr5:24594715-24594734	None:intergenic	30.0%
ATTAGGAAAATTAGCGATTC+AGG	+	Chr5:24592978-24592997	MsG0580025814.01.T01:CDS	30.0%
CAAACCCTTGAATATAAGTT+TGG	-	Chr5:24593163-24593182	None:intergenic	30.0%
CTATTGACATAAGCATTTGT+AGG	+	Chr5:24593214-24593233	MsG0580025814.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	24592553	24595526	24592553	ID=MsG0580025814.01;Name=MsG0580025814.01
Chr5	mRNA	24592553	24595526	24592553	ID=MsG0580025814.01.T01;Parent=MsG0580025814.01;Name=MsG0580025814.01.T01;_AED=0.33;_eAED=0.33;_QI=0\|1\|0.8\|1\|1\|1\|5\|227\|502
Chr5	exon	24592553	24592736	24592553	ID=MsG0580025814.01.T01:exon:3920;Parent=MsG0580025814.01.T01
Chr5	exon	24592829	24592999	24592829	ID=MsG0580025814.01.T01:exon:3921;Parent=MsG0580025814.01.T01
Chr5	exon	24593549	24593761	24593549	ID=MsG0580025814.01.T01:exon:3922;Parent=MsG0580025814.01.T01
Chr5	exon	24593841	24593938	24593841	ID=MsG0580025814.01.T01:exon:3923;Parent=MsG0580025814.01.T01
Chr5	exon	24594457	24595526	24594457	ID=MsG0580025814.01.T01:exon:3924;Parent=MsG0580025814.01.T01
Chr5	CDS	24592553	24592736	24592553	ID=MsG0580025814.01.T01:cds;Parent=MsG0580025814.01.T01
Chr5	CDS	24592829	24592999	24592829	ID=MsG0580025814.01.T01:cds;Parent=MsG0580025814.01.T01
Chr5	CDS	24593549	24593761	24593549	ID=MsG0580025814.01.T01:cds;Parent=MsG0580025814.01.T01
Chr5	CDS	24593841	24593938	24593841	ID=MsG0580025814.01.T01:cds;Parent=MsG0580025814.01.T01
Chr5	CDS	24594457	24595299	24594457	ID=MsG0580025814.01.T01:cds;Parent=MsG0580025814.01.T01
Chr5	three_prime_UTR	24595300	24595526	24595300	ID=MsG0580025814.01.T01:three_prime_utr;Parent=MsG0580025814.01.T01

Gene Sequence

>MsG0580025814.01.T01

ATGACAGCGATTGCTGGGAAGTCAATGATGAAGTTTGAGAGTGGATACAATGTGGAAACTGTGTTTGATGGAAGCAAGCTTGGAATTGAGCCTTATGCAGTTGAGGTGTTGTCTAATGGGGAGCTTCTGATTCTGGATTCGGAGAATAGTAACATTTATAAGATTTCATCTTCACTTTCGTTGTATAGCAGGCCAAAACTTGTGGCAGGATCTGCTGAAGGATATTCTGGACACGTTGATGGAAAATTAAGAGAGGCTAGGATGAACCACCCGAAGGGGATTACTGTTGATGACCGAGGAAATATCTATGTTGCAGATATTATGAATATGGCAATTAGGAAAATTAGCGATTCAGGGGTTACGACAATTGCTGGGGGAAAGTTAAGCCGTGGAGGGGGCCATGTCGATGGACCAAGTGAAGAGGCTAAATTTTCTTATGACTTTGATGTGGTTTATGTTGGAAGTAGTTGTTCCCTACTTGTCATTGACAGGGGGAATCAAGCCATCAGGGAGATTCAGCTGCGCTTTGATGAGTGTGCTTATCAATACGAAAGTGGATTTCCACTCGGAATTGCAATGCTTCTCGGTGCTGGCTTCTTTGGTTATATGCTGGCATTGCTGCAGCGTCGTCTAAGTACAATTGTCGCATCTCAGGATGTGACTCATGCTGAGAGCTCAGCGATGTCTGACTTTTCACCGAGTCTTTACCAAAAGCCGTTGAAATCTGTCAGACCGCCTTTGATTCCATCAGAAGACGAATCTTATAAGCAAGAAGAAGGTCTCTTTGCATCCATTGGGAAGCTTCTTACCAATGCTGGGGCCTCAGTAGTGGAAATAATGGGTTTCAGGAAAAAACCGCAAAGCTATGAATTTCAAAGCCAACCTTTATTCCATCAACCTGAAAGGCAAATAAATGCTTGGCCTGTGCAAGAAAGTTTTGTAATTCCAAATGAAGACGAGCCTCCTTCTATTGACCCAAGAACTCCAACCCCTAAGAAAACATATCCTTTCATGATTAAAGACACCGAAAAAATGCAACAATTGTGGCAAGGTAGAGCGCTCTATTATGGATGGGAAGGAGATCTTCAGCATCAGCAGCAGCAACCACTAAAACATGTGTACAATGGATGGGAAGGTGATCTTCATCAGCAGACGCAGCAACAACAACAGCAACCACAACAAAAACATCTGTATAACGGATGGGTCGGAGATCTTCAGAAGCAGCCGCAACAACAACAACAACAGCAACCACAACAAAAACATCTGTATAACGGATGGGACGGAGATCTTCAGCAGCAGCAAAAACATCATTATCGTAATCAGTATCATTCATCGGTCGCTCATACTTACTATGAGCAAAGTCGCGAGGAAACCAATGAGATAGTATTTGGTGCAGTGCAGGAACAGGATGAGAAGGAGTCTGTAGTTATCAAGCCTTTGGATTACGGTGATTCTCTCTATGATCATCATAATATGCGGTCTCGAATCAGTTATATCCACAAGTACTGA

Protein sequence

>MsG0580025814.01.T01

MTAIAGKSMMKFESGYNVETVFDGSKLGIEPYAVEVLSNGELLILDSENSNIYKISSSLSLYSRPKLVAGSAEGYSGHVDGKLREARMNHPKGITVDDRGNIYVADIMNMAIRKISDSGVTTIAGGKLSRGGGHVDGPSEEAKFSYDFDVVYVGSSCSLLVIDRGNQAIREIQLRFDECAYQYESGFPLGIAMLLGAGFFGYMLALLQRRLSTIVASQDVTHAESSAMSDFSPSLYQKPLKSVRPPLIPSEDESYKQEEGLFASIGKLLTNAGASVVEIMGFRKKPQSYEFQSQPLFHQPERQINAWPVQESFVIPNEDEPPSIDPRTPTPKKTYPFMIKDTEKMQQLWQGRALYYGWEGDLQHQQQQPLKHVYNGWEGDLHQQTQQQQQQPQQKHLYNGWVGDLQKQPQQQQQQQPQQKHLYNGWDGDLQQQQKHHYRNQYHSSVAHTYYEQSREETNEIVFGAVQEQDEKESVVIKPLDYGDSLYDHHNMRSRISYIHKY*