AlfalfaGEDB | MsG0480018124.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018124.01

KEGG: K02135
GO: GO:0000275 GO:0015986 GO:0046933
PFAM: PF04627

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018124.01.T01	AES86345.1	86	50	7	0	1	50	9	58	1.44E-22	92.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018124.01.T01	Q96253	52.083	48	21	1	1	48	15	60	7.75E-12	57.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018124.01.T01	G7JVK8	86.000	50	7	0	1	50	9	58	6.88e-23	92.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048784.01	MsG0480018124.01	0.822498	2.293721e-53	1.923195e-50
MsG0080048856.01	MsG0480018124.01	0.821519	3.868061e-53	3.153896e-50
MsG0180000139.01	MsG0480018124.01	0.875262	3.538683e-68	1.641899e-64
MsG0180000331.01	MsG0480018124.01	0.814808	1.275616e-51	8.645424e-49
MsG0180000335.01	MsG0480018124.01	0.800192	1.617201e-48	7.483149e-46
MsG0180000614.01	MsG0480018124.01	0.818276	2.133146e-52	1.589534e-49
MsG0180000835.01	MsG0480018124.01	0.803006	4.283351e-49	2.128614e-46
MsG0180001141.01	MsG0480018124.01	0.806052	9.920698e-50	5.331993e-47
MsG0180001356.01	MsG0480018124.01	0.801588	8.387652e-49	4.020694e-46
MsG0180002547.01	MsG0480018124.01	0.826372	2.812491e-54	2.632787e-51
MsG0180002794.01	MsG0480018124.01	0.835369	1.746667e-56	2.131640e-53
MsG0180003493.01	MsG0480018124.01	0.810971	8.839542e-51	5.405261e-48
MsG0180003651.01	MsG0480018124.01	0.888790	4.319056e-73	3.393132e-69
MsG0180003793.01	MsG0480018124.01	0.833720	4.534828e-56	5.266342e-53
MsG0180004130.01	MsG0480018124.01	0.817496	3.200338e-52	2.334400e-49
MsG0180004152.01	MsG0480018124.01	0.829733	4.364589e-55	4.506140e-52
MsG0180004463.01	MsG0480018124.01	0.801636	8.202246e-49	3.936639e-46
MsG0180004481.01	MsG0480018124.01	0.873033	2.011147e-67	8.600411e-64
MsG0180004482.01	MsG0480018124.01	0.877274	7.164541e-69	3.581467e-65
MsG0180004509.01	MsG0480018124.01	0.832044	1.183411e-55	1.307474e-52
MsG0180004765.01	MsG0480018124.01	0.807626	4.610961e-50	2.580794e-47
MsG0180004854.01	MsG0480018124.01	0.851635	7.807824e-61	1.590309e-57
MsG0180004928.01	MsG0480018124.01	0.807893	4.046156e-50	2.280406e-47
MsG0180004975.01	MsG0480018124.01	0.805549	1.265164e-49	6.712381e-47
MsG0180005153.01	MsG0480018124.01	0.815977	7.011967e-52	4.906246e-49
MsG0180005169.01	MsG0480018124.01	0.804452	2.145296e-49	1.106507e-46
MsG0180005659.01	MsG0480018124.01	0.836443	9.331325e-57	1.176156e-53
MsG0180005763.01	MsG0480018124.01	0.823017	1.736887e-53	1.477938e-50
MsG0180006017.01	MsG0480018124.01	0.805441	1.333257e-49	7.054059e-47
MsG0180006096.01	MsG0480018124.01	0.801654	8.132474e-49	3.904873e-46
MsG0280006405.01	MsG0480018124.01	0.818031	2.423023e-52	1.793447e-49
MsG0280006406.01	MsG0480018124.01	0.840346	9.188128e-58	1.305008e-54
MsG0280006422.01	MsG0480018124.01	0.802025	6.822930e-49	3.307203e-46
MsG0280010776.01	MsG0480018124.01	0.843215	1.607320e-58	2.496978e-55
MsG0280010778.01	MsG0480018124.01	0.831543	1.573170e-55	1.712607e-52
MsG0280010980.01	MsG0480018124.01	0.816555	5.205444e-52	3.700261e-49
MsG0280011014.01	MsG0480018124.01	0.808976	2.376189e-50	1.378004e-47
MsG0280011304.01	MsG0480018124.01	0.801101	1.055240e-48	4.997155e-46
MsG0280011339.01	MsG0480018124.01	0.812884	3.386349e-51	2.178521e-48
MsG0280011387.01	MsG0480018124.01	0.833484	5.193983e-56	5.989640e-53
MsG0280011388.01	MsG0480018124.01	0.840174	1.019101e-57	1.439922e-54
MsG0380011581.01	MsG0480018124.01	0.801068	1.071823e-48	5.071304e-46
MsG0380012122.01	MsG0480018124.01	0.822613	2.157146e-53	1.814782e-50
MsG0380013085.01	MsG0480018124.01	0.815891	7.326351e-52	5.113951e-49
MsG0380013178.01	MsG0480018124.01	0.813501	2.479807e-51	1.622227e-48
MsG0380014703.01	MsG0480018124.01	0.800515	1.390301e-48	6.486258e-46
MsG0380014876.01	MsG0480018124.01	0.806492	8.013416e-50	4.355409e-47
MsG0380015435.01	MsG0480018124.01	0.846226	2.481646e-59	4.241886e-56
MsG0380015466.01	MsG0480018124.01	0.811706	6.123491e-51	3.817243e-48
MsG0380016126.01	MsG0480018124.01	0.815371	9.568028e-52	6.584572e-49
MsG0380016290.01	MsG0480018124.01	0.835631	1.499467e-56	1.844151e-53
MsG0380016505.01	MsG0480018124.01	0.810302	1.233319e-50	7.407317e-48
MsG0380016559.01	MsG0480018124.01	0.813983	1.941927e-51	1.286972e-48
MsG0380016560.01	MsG0480018124.01	0.827853	1.243748e-54	1.215349e-51
MsG0380016671.01	MsG0480018124.01	0.839311	1.709776e-57	2.351936e-54
MsG0380016860.01	MsG0480018124.01	0.800262	1.565494e-48	7.256885e-46
MsG0380017461.01	MsG0480018124.01	0.802959	4.378817e-49	2.173622e-46
MsG0380017857.01	MsG0480018124.01	0.815539	8.776888e-52	6.068163e-49
MsG0380017947.01	MsG0480018124.01	0.809834	1.555830e-50	9.231164e-48
MsG0480018110.01	MsG0480018124.01	0.821777	3.370329e-53	2.768401e-50
MsG0480018124.01	MsG0480018159.01	0.813037	3.134320e-51	2.024658e-48
MsG0480018124.01	MsG0480018278.01	0.816842	4.489357e-52	3.216506e-49
MsG0480018124.01	MsG0480018477.01	0.830274	3.220739e-55	3.377227e-52
MsG0480018124.01	MsG0480018894.01	0.820135	8.047730e-53	6.315158e-50
MsG0480018124.01	MsG0480018911.01	0.801160	1.026441e-48	4.867803e-46
MsG0480018124.01	MsG0480020136.01	0.808957	2.399600e-50	1.390822e-47
MsG0480018124.01	MsG0480020739.01	0.802585	5.232049e-49	2.572512e-46
MsG0480018124.01	MsG0480020879.01	0.813130	2.990790e-51	1.936683e-48
MsG0480018124.01	MsG0480021006.01	0.811447	6.971077e-51	4.316253e-48
MsG0480018124.01	MsG0480021037.01	0.820070	8.331085e-53	6.525633e-50
MsG0480018124.01	MsG0480021706.01	0.805349	1.393721e-49	7.356562e-47
MsG0480018124.01	MsG0480021750.01	0.803690	3.090799e-49	1.563297e-46
MsG0480018124.01	MsG0480021768.01	0.803769	2.976008e-49	1.508303e-46
MsG0480018124.01	MsG0480022696.01	0.814259	1.687398e-51	1.126766e-48
MsG0480018124.01	MsG0480022711.01	0.811905	5.540811e-51	3.472309e-48
MsG0480018124.01	MsG0480022877.01	0.816449	5.497962e-52	3.896671e-49
MsG0480018124.01	MsG0480023163.01	0.806470	8.099955e-50	4.400062e-47
MsG0480018124.01	MsG0480023221.01	0.858843	6.238011e-63	1.614142e-59
MsG0480018124.01	MsG0480023361.01	0.819416	1.175092e-52	9.037042e-50
MsG0480018124.01	MsG0480023463.01	0.825911	3.619688e-54	3.344251e-51
MsG0480018124.01	MsG0480023814.01	0.820235	7.636028e-53	6.007916e-50
MsG0480018124.01	MsG0580024161.01	0.809646	1.707427e-50	1.008093e-47
MsG0480018124.01	MsG0580024197.01	0.830675	2.569048e-55	2.725757e-52
MsG0480018124.01	MsG0580024390.01	0.812467	4.178554e-51	2.659011e-48
MsG0480018124.01	MsG0580025135.01	0.810650	1.037235e-50	6.287882e-48
MsG0480018124.01	MsG0580025268.01	0.844685	6.486474e-59	1.055352e-55
MsG0480018124.01	MsG0580025587.01	0.823176	1.594933e-53	1.363160e-50
MsG0480018124.01	MsG0580025814.01	0.820793	5.684019e-53	4.542054e-50
MsG0480018124.01	MsG0580027361.01	0.837668	4.536517e-57	5.935658e-54
MsG0480018124.01	MsG0580027999.01	0.845922	3.001466e-59	5.080989e-56
MsG0480018124.01	MsG0580028630.01	0.816293	5.958123e-52	4.205441e-49
MsG0480018124.01	MsG0580029729.01	0.804889	1.739032e-49	9.072553e-47
MsG0480018124.01	MsG0680030350.01	0.802086	6.629999e-49	3.218717e-46
MsG0480018124.01	MsG0680030882.01	0.813673	2.271932e-51	1.493179e-48
MsG0480018124.01	MsG0680031488.01	0.814011	1.914732e-51	1.269970e-48
MsG0480018124.01	MsG0680031628.01	0.837361	5.438756e-57	7.047610e-54
MsG0480018124.01	MsG0680035888.01	0.836676	8.137050e-57	1.032785e-53
MsG0480018124.01	MsG0780036240.01	0.827353	1.639608e-54	1.579165e-51
MsG0480018124.01	MsG0780036486.01	0.807718	4.407377e-50	2.472822e-47
MsG0480018124.01	MsG0780036964.01	0.823926	1.064805e-53	9.295824e-51
MsG0480018124.01	MsG0780037054.01	0.807101	5.956869e-50	3.288815e-47
MsG0480018124.01	MsG0780037093.01	0.829386	5.298291e-55	5.414823e-52
MsG0480018124.01	MsG0780037240.01	0.827160	1.823774e-54	1.746455e-51
MsG0480018124.01	MsG0780038400.01	0.848852	4.704806e-60	8.751386e-57
MsG0480018124.01	MsG0780040057.01	0.800038	1.738167e-48	8.011550e-46
MsG0480018124.01	MsG0780040718.01	0.808804	2.587480e-50	1.493602e-47
MsG0480018124.01	MsG0780040734.01	0.827740	1.324275e-54	1.289668e-51
MsG0480018124.01	MsG0780040926.01	0.808516	2.981104e-50	1.707994e-47
MsG0480018124.01	MsG0780041016.01	0.807828	4.177367e-50	2.350415e-47
MsG0480018124.01	MsG0780041137.01	0.834026	3.803194e-56	4.457717e-53
MsG0480018124.01	MsG0780041223.01	0.833858	4.189507e-56	4.886243e-53
MsG0480018124.01	MsG0780041391.01	0.813382	2.633297e-51	1.717083e-48
MsG0480018124.01	MsG0780041435.01	0.809167	2.163020e-50	1.260846e-47
MsG0480018124.01	MsG0780041440.01	0.807910	4.011789e-50	2.262052e-47
MsG0480018124.01	MsG0780041462.01	0.800380	1.481061e-48	6.886235e-46
MsG0480018124.01	MsG0880041986.01	0.804031	2.625514e-49	1.339669e-46
MsG0480018124.01	MsG0880042128.01	0.853942	1.711481e-61	3.760543e-58
MsG0480018124.01	MsG0880042129.01	0.843436	1.403285e-58	2.195421e-55
MsG0480018124.01	MsG0880042519.01	0.817045	4.041878e-52	2.912119e-49
MsG0480018124.01	MsG0880043479.01	0.802401	5.709956e-49	2.794471e-46
MsG0480018124.01	MsG0880044975.01	0.808635	2.811299e-50	1.615640e-47
MsG0480018124.01	MsG0880045145.01	0.811934	5.461033e-51	3.424937e-48
MsG0480018124.01	MsG0880045294.01	0.823225	1.552906e-53	1.329063e-50
MsG0480018124.01	MsG0880045535.01	0.822931	1.818780e-53	1.543851e-50
MsG0480018124.01	MsG0880045684.01	0.800270	1.559025e-48	7.228500e-46
MsG0480018124.01	MsG0880045765.01	0.801764	7.718591e-49	3.716510e-46
MsG0480018124.01	MsG0880047427.01	0.833909	4.067562e-56	4.751558e-53
MsG0480018124.01	MsG0880047448.01	0.807409	5.127385e-50	2.853779e-47
MsG0480018124.01	MsG0880047473.01	0.800246	1.577034e-48	7.307335e-46
MsG0280006993.01	MsG0480018124.01	0.864863	8.953212e-65	2.852970e-61
MsG0280007527.01	MsG0480018124.01	0.812471	4.169575e-51	2.653590e-48
MsG0280007847.01	MsG0480018124.01	0.800564	1.358546e-48	6.345932e-46
MsG0280008429.01	MsG0480018124.01	0.813840	2.087697e-51	1.378279e-48
MsG0280009575.01	MsG0480018124.01	0.801790	7.624346e-49	3.673659e-46
MsG0280009794.01	MsG0480018124.01	0.810887	9.217074e-51	5.623289e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018124.01.T01	MTR_4g005960	86.000	50	7	0	1	50	9	58	1.75e-26	92.4
MsG0480018124.01.T01	MTR_5g040610	53.448	58	22	2	1	55	15	70	3.42e-14	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018124.01.T01	AT1G51650	52.083	48	21	1	1	48	15	60	7.90e-13	57.8

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTGACAGCCAATCTTATT+AGG	0.245215	4:+482481	MsG0480018124.01.T01:CDS
CTTCATCACTTGGGTTGACC+TGG	0.350496	4:+482661	MsG0480018124.01.T01:CDS
AAGAAGAGAGAAAGAGAAAG+AGG	0.379706	4:-482616	None:intergenic
AAGGCAATTCCTAATAAGAT+TGG	0.392366	4:-482490	None:intergenic
TTTACCCTTCCTTCATCACT+TGG	0.402403	4:+482651	MsG0480018124.01.T01:CDS
TCTTATTAGGAATTGCCTTA+AGG	0.405336	4:+482494	MsG0480018124.01.T01:CDS
AATAAGATTGGCTGTCAAGT+TGG	0.465236	4:-482478	None:intergenic
AGAAGAGAGAAAGAGAAAGA+GGG	0.473593	4:-482615	None:intergenic
ACATGGATCGACAGTCATTC+CGG	0.483118	4:+482576	MsG0480018124.01.T01:CDS
GAAGAGAGAAAGAGAAAGAG+GGG	0.503813	4:-482614	None:intergenic
CAGGTCAACCCAAGTGATGA+AGG	0.518441	4:-482660	None:intergenic
TTCAGTACTTGCTTCCCACA+TGG	0.547985	4:+482559	MsG0480018124.01.T01:CDS
TTACCCTTCCTTCATCACTT+GGG	0.549058	4:+482652	MsG0480018124.01.T01:CDS
TCAACCCAAGTGATGAAGGA+AGG	0.576625	4:-482656	None:intergenic
CAACCCAAGTGATGAAGGAA+GGG	0.606003	4:-482655	None:intergenic
CAAGTTCCGAAGCTAAGACC+AGG	0.614577	4:-482679	None:intergenic
AAGAGAGAAAGAGAAAGAGG+GGG	0.617518	4:-482613	None:intergenic
GAATGACTGTCGATCCATGT+GGG	0.656324	4:-482573	None:intergenic
GGAATGACTGTCGATCCATG+TGG	0.711549	4:-482574	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGGCAATTCCTAATAAGAT+TGG	-	Chr4:482493-482512	None:intergenic	30.0%
	TCTTATTAGGAATTGCCTTA+AGG	+	Chr4:482494-482513	MsG0480018124.01.T01:CDS	30.0%
	AAGAAGAGAGAAAGAGAAAG+AGG	-	Chr4:482619-482638	None:intergenic	35.0%
	ACTTGACAGCCAATCTTATT+AGG	+	Chr4:482481-482500	MsG0480018124.01.T01:CDS	35.0%
	AGAAGAGAGAAAGAGAAAGA+GGG	-	Chr4:482618-482637	None:intergenic	35.0%
!	AATAAGATTGGCTGTCAAGT+TGG	-	Chr4:482481-482500	None:intergenic	35.0%
!!	GAGAGAATTTGAGTTTTGTG+AGG	-	Chr4:482522-482541	None:intergenic	35.0%
	AAGAGAGAAAGAGAAAGAGG+GGG	-	Chr4:482616-482635	None:intergenic	40.0%
	GAAGAGAGAAAGAGAAAGAG+GGG	-	Chr4:482617-482636	None:intergenic	40.0%
	TTACCCTTCCTTCATCACTT+GGG	+	Chr4:482652-482671	MsG0480018124.01.T01:CDS	40.0%
	TTTACCCTTCCTTCATCACT+TGG	+	Chr4:482651-482670	MsG0480018124.01.T01:CDS	40.0%
!!	GAGTTTTGTGAGGTTCCTTA+AGG	-	Chr4:482512-482531	None:intergenic	40.0%
	ACATGGATCGACAGTCATTC+CGG	+	Chr4:482576-482595	MsG0480018124.01.T01:CDS	45.0%
	CAACCCAAGTGATGAAGGAA+GGG	-	Chr4:482658-482677	None:intergenic	45.0%
	GAATGACTGTCGATCCATGT+GGG	-	Chr4:482576-482595	None:intergenic	45.0%
	TCAACCCAAGTGATGAAGGA+AGG	-	Chr4:482659-482678	None:intergenic	45.0%
	TTCAGTACTTGCTTCCCACA+TGG	+	Chr4:482559-482578	MsG0480018124.01.T01:CDS	45.0%
	CAGGTCAACCCAAGTGATGA+AGG	-	Chr4:482663-482682	None:intergenic	50.0%
	GGAATGACTGTCGATCCATG+TGG	-	Chr4:482577-482596	None:intergenic	50.0%
!	GGGGGTCTTACTGAGTTTTC+CGG	-	Chr4:482598-482617	None:intergenic	50.0%
!!	CTTCATCACTTGGGTTGACC+TGG	+	Chr4:482661-482680	MsG0480018124.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	482459	482689	482459	ID=MsG0480018124.01;Name=MsG0480018124.01
Chr4	mRNA	482459	482689	482459	ID=MsG0480018124.01.T01;Parent=MsG0480018124.01;Name=MsG0480018124.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|76
Chr4	exon	482459	482689	482459	ID=MsG0480018124.01.T01:exon:651;Parent=MsG0480018124.01.T01
Chr4	CDS	482459	482689	482459	ID=MsG0480018124.01.T01:cds;Parent=MsG0480018124.01.T01

Gene Sequence	>MsG0480018124.01.T01 ATGACATACACAGCATATTCCAACTTGACAGCCAATCTTATTAGGAATTGCCTTAAGGAACCTCACAAAACTCAAATTCTCTCTCTTTCTCGCGAGAACGTTCAGTACTTGCTTCCCACATGGATCGACAGTCATTCCGGAAAACTCAGTAAGACCCCCTCTTTCTCTTTCTCTCTTCTTCTATTTACTATCTTTACCCTTCCTTCATCACTTGGGTTGACCTGGTCTTAG
Protein sequence	>MsG0480018124.01.T01 MTYTAYSNLTANLIRNCLKEPHKTQILSLSRENVQYLLPTWIDSHSGKLSKTPSFSFSLLLFTIFTLPSSLGLTWS*