AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030886.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030886.01.T01 MTR_6g013820 55.639 133 13 1 4 136 31 117 1.35e-38 127
MsG0680030886.01.T01 MTR_7g012560 63.014 73 25 1 4 74 31 103 1.27e-23 88.6
MsG0680030886.01.T01 MTR_7g012560 61.972 71 25 1 4 72 31 101 6.12e-22 84.7
MsG0680030886.01.T01 MTR_7g027300 60.784 51 20 0 17 67 43 93 1.11e-13 63.5
MsG0680030886.01.T01 MTR_7g027017 51.562 64 31 0 4 67 30 93 1.04e-12 63.9
MsG0680030886.01.T01 MTR_8g096380 57.143 49 21 0 19 67 45 93 1.32e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030886.01.T01 AT5G15460 58.730 63 26 0 4 66 31 93 2.79e-18 75.5
MsG0680030886.01.T01 AT5G15460 58.730 63 26 0 4 66 31 93 2.79e-18 75.5
MsG0680030886.01.T01 AT5G15460 58.730 63 26 0 4 66 31 93 2.79e-18 75.5
MsG0680030886.01.T01 AT3G01050 52.174 69 33 0 4 72 31 99 1.14e-17 73.6
MsG0680030886.01.T01 AT3G01050 52.174 69 33 0 4 72 31 99 1.14e-17 73.6
MsG0680030886.01.T01 AT3G01050 52.174 69 33 0 4 72 31 99 1.14e-17 73.6
MsG0680030886.01.T01 AT3G01050 52.174 69 33 0 4 72 31 99 1.14e-17 73.6
MsG0680030886.01.T01 AT3G01050 52.174 69 33 0 4 72 31 99 1.14e-17 73.6
MsG0680030886.01.T01 AT5G15460 70.833 48 14 0 19 66 20 67 5.20e-17 71.6
MsG0680030886.01.T01 AT1G22050 49.296 71 36 0 2 72 29 99 3.54e-16 70.1
MsG0680030886.01.T01 AT1G77870 46.575 73 37 1 2 72 28 100 1.39e-12 60.5
MsG0680030886.01.T01 AT3G26980 58.000 50 21 0 17 66 43 92 4.74e-12 59.3
MsG0680030886.01.T01 AT4G24990 60.000 45 18 0 23 67 49 93 6.18e-12 58.9
MsG0680030886.01.T01 AT4G24990 60.000 45 18 0 23 67 49 93 6.18e-12 58.9

Find 26 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTGTGCAACCACCTAATT+TGG 0.215657 6:-11123669 MsG0680030886.01.T01:CDS
AAAGAAATTTCCATTGTCTA+TGG 0.289355 6:-11123265 MsG0680030886.01.T01:three_prime_UTR
AAGAACGTGAGTTCGATTTC+TGG 0.305977 6:+11123519 None:intergenic
CACAAACCTTTGTCCAAATT+AGG 0.314428 6:+11123656 None:intergenic
TTCCTCATACACAAGTTCTT+TGG 0.334891 6:-11123192 MsG0680030886.01.T01:three_prime_UTR
CACATTCTGGTTGTTTCACA+TGG 0.338531 6:-11123569 MsG0680030886.01.T01:CDS
GATTTCTGGAGTCGCTCATT+TGG 0.338875 6:+11123533 None:intergenic
ATGTATGTGTTGGCACATTC+TGG 0.357601 6:-11123582 MsG0680030886.01.T01:CDS
ATTTCTGGAGTCGCTCATTT+GGG 0.368591 6:+11123534 None:intergenic
GACAAAGGTTTGTGTGTGTT+TGG 0.373395 6:-11123647 MsG0680030886.01.T01:CDS
AGTCCATTGTGTAATCTTCC+TGG 0.429392 6:-11123713 MsG0680030886.01.T01:CDS
AACCAAAGAACTTGTGTATG+AGG 0.467564 6:+11123190 None:intergenic
GATGTGAAGTTAATTTGTGC+TGG 0.476748 6:-11123776 MsG0680030886.01.T01:CDS
TCTGGTTGTTTCACATGGGT+CGG 0.511095 6:-11123564 MsG0680030886.01.T01:CDS
AAAATTGTGTGAAGAAACAC+TGG 0.551532 6:+11123107 None:intergenic
CAACCACCTAATTTGGACAA+AGG 0.572921 6:-11123662 MsG0680030886.01.T01:CDS
TCTGGAGTCGCTCATTTGGG+TGG 0.577282 6:+11123537 None:intergenic
ACATTCTGGTTGTTTCACAT+GGG 0.580287 6:-11123568 MsG0680030886.01.T01:CDS
AGCATTGTTGTAACTCCACC+AGG 0.597774 6:+11123695 None:intergenic
AAACCTTTGTCCAAATTAGG+TGG 0.603121 6:+11123659 None:intergenic
CCATTGTGTAATCTTCCTGG+TGG 0.622842 6:-11123710 MsG0680030886.01.T01:CDS
GTGACTTTAACCATAGACAA+TGG 0.625666 6:+11123255 None:intergenic
GGAGAACAACAAAACAGTAG+AGG 0.643312 6:-11123744 MsG0680030886.01.T01:CDS
ATGTGTTAAAATGTATGTGT+TGG 0.644083 6:-11123592 MsG0680030886.01.T01:CDS
TGGAGTTACAACAATGCTTG+TGG 0.673028 6:-11123690 MsG0680030886.01.T01:CDS
CCACCAGGAAGATTACACAA+TGG 0.747125 6:+11123710 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAGTTCATATAGTTT+TGG - Chr6:11123440-11123459 MsG0680030886.01.T01:intron 15.0%
!!! TTTTACATTGTAATAGTATA+AGG + Chr6:11123719-11123738 None:intergenic 15.0%
!! ATGTAAAAAGTTATTGAGTT+TGG - Chr6:11123731-11123750 MsG0680030886.01.T01:CDS 20.0%
!!! ATCATTTTTATCATTGTCTA+TGG - Chr6:11123652-11123671 MsG0680030886.01.T01:CDS 20.0%
!!! TTTTAACACATTATAATTGC+AGG + Chr6:11123277-11123296 None:intergenic 20.0%
!!! TTTTTTGAAGATAAAGAGTA+TGG - Chr6:11123063-11123082 MsG0680030886.01.T01:three_prime_UTR 20.0%
! AAAGAAATTTCCATTGTCTA+TGG - Chr6:11123613-11123632 MsG0680030886.01.T01:CDS 25.0%
! AATTTGTGCTGGAAAAATAT+TGG - Chr6:11123113-11123132 MsG0680030886.01.T01:three_prime_UTR 25.0%
! ATGTGTTAAAATGTATGTGT+TGG - Chr6:11123286-11123305 MsG0680030886.01.T01:three_prime_UTR 25.0%
!! TTCACATCTTTTACAGTTTT+TGG + Chr6:11123090-11123109 None:intergenic 25.0%
!!! TTTGATTCTCATTGATTTTC+TGG - Chr6:11123470-11123489 MsG0680030886.01.T01:intron 25.0%
AAAATTGTGTGAAGAAACAC+TGG + Chr6:11123774-11123793 None:intergenic 30.0%
! GTGTGTGTTTGGTATTTTAT+TGG - Chr6:11123242-11123261 MsG0680030886.01.T01:three_prime_UTR 30.0%
AAACCTTTGTCCAAATTAGG+TGG + Chr6:11123222-11123241 None:intergenic 35.0%
AAAGACATTGTTACGAGAGT+TGG + Chr6:11123407-11123426 None:intergenic 35.0%
AACCAAAGAACTTGTGTATG+AGG + Chr6:11123691-11123710 None:intergenic 35.0%
CACAAACCTTTGTCCAAATT+AGG + Chr6:11123225-11123244 None:intergenic 35.0%
GTGACTTTAACCATAGACAA+TGG + Chr6:11123626-11123645 None:intergenic 35.0%
TTCCTCATACACAAGTTCTT+TGG - Chr6:11123686-11123705 MsG0680030886.01.T01:CDS 35.0%
! ACATTCTGGTTGTTTCACAT+GGG - Chr6:11123310-11123329 MsG0680030886.01.T01:three_prime_UTR 35.0%
!! GATGTGAAGTTAATTTGTGC+TGG - Chr6:11123102-11123121 MsG0680030886.01.T01:three_prime_UTR 35.0%
AAGAACGTGAGTTCGATTTC+TGG + Chr6:11123362-11123381 None:intergenic 40.0%
AGTCCATTGTGTAATCTTCC+TGG - Chr6:11123165-11123184 MsG0680030886.01.T01:three_prime_UTR 40.0%
CAACCACCTAATTTGGACAA+AGG - Chr6:11123216-11123235 MsG0680030886.01.T01:three_prime_UTR 40.0%
GACAAAGGTTTGTGTGTGTT+TGG - Chr6:11123231-11123250 MsG0680030886.01.T01:three_prime_UTR 40.0%
GGAGAACAACAAAACAGTAG+AGG - Chr6:11123134-11123153 MsG0680030886.01.T01:three_prime_UTR 40.0%
TGGAGTTACAACAATGCTTG+TGG - Chr6:11123188-11123207 MsG0680030886.01.T01:three_prime_UTR 40.0%
! ATGTATGTGTTGGCACATTC+TGG - Chr6:11123296-11123315 MsG0680030886.01.T01:three_prime_UTR 40.0%
! CACATTCTGGTTGTTTCACA+TGG - Chr6:11123309-11123328 MsG0680030886.01.T01:three_prime_UTR 40.0%
!! ATTTCTGGAGTCGCTCATTT+GGG + Chr6:11123347-11123366 None:intergenic 40.0%
AGCATTGTTGTAACTCCACC+AGG + Chr6:11123186-11123205 None:intergenic 45.0%
CCACCAGGAAGATTACACAA+TGG + Chr6:11123171-11123190 None:intergenic 45.0%
CCATTGTGTAATCTTCCTGG+TGG - Chr6:11123168-11123187 MsG0680030886.01.T01:three_prime_UTR 45.0%
GGTTGTGCAACCACCTAATT+TGG - Chr6:11123209-11123228 MsG0680030886.01.T01:three_prime_UTR 45.0%
! TCTGGTTGTTTCACATGGGT+CGG - Chr6:11123314-11123333 MsG0680030886.01.T01:exon 45.0%
!! GATTTCTGGAGTCGCTCATT+TGG + Chr6:11123348-11123367 None:intergenic 45.0%
! TCTGGAGTCGCTCATTTGGG+TGG + Chr6:11123344-11123363 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 11123021 11123879 11123021 ID=MsG0680030886.01;Name=MsG0680030886.01
Chr6 mRNA 11123021 11123879 11123021 ID=MsG0680030886.01.T01;Parent=MsG0680030886.01;Name=MsG0680030886.01.T01;_AED=0.49;_eAED=0.54;_QI=0|0|0.5|1|0|0|2|312|136
Chr6 exon 11123021 11123388 11123021 ID=MsG0680030886.01.T01:exon:8266;Parent=MsG0680030886.01.T01
Chr6 exon 11123525 11123879 11123525 ID=MsG0680030886.01.T01:exon:8265;Parent=MsG0680030886.01.T01
Chr6 CDS 11123525 11123879 11123525 ID=MsG0680030886.01.T01:cds;Parent=MsG0680030886.01.T01
Chr6 CDS 11123333 11123388 11123333 ID=MsG0680030886.01.T01:cds;Parent=MsG0680030886.01.T01
Chr6 three_prime_UTR 11123021 11123332 11123021 ID=MsG0680030886.01.T01:three_prime_utr;Parent=MsG0680030886.01.T01
Gene Sequence

>MsG0680030886.01.T01

ATGCTTTTTGTTATATATGTGAAATTTATATGTTTGTGTTTCTTTTTTGAAGATAAAGAGTATGGTCCAAAAACTGTAAAAGATGTGAAGTTAATTTGTGCTGGAAAAATATTGGAGAACAACAAAACAGTAGAGGAATGTCAGAGTCCATTGTGTAATCTTCCTGGTGGAGTTACAACAATGCTTGTGGTTGTGCAACCACCTAATTTGGACAAAGGTTTGTGTGTGTTTGGTATTTTATTGGTCATTGATTCCTGCAATTATAATGTGTTAAAATGTATGTGTTGGCACATTCTGGTTGTTTCACATGGGTCGGATTGCCACCCAAATGAGCGACTCCAGAAATCGAACTCACATAAGAAAGTAGCAGATGAAGCAATGCAAAGCAAATGCGTTTGTGTTATATTATAG

Protein sequence

>MsG0680030886.01.T01

MLFVIYVKFICLCFFFEDKEYGPKTVKDVKLICAGKILENNKTVEECQSPLCNLPGGVTTMLVVVQPPNLDKGLCVFGILLVIDSCNYNVLKCMCWHILVVSHGSDCHPNERLQKSNSHKKVADEAMQSKCVCVIL*