Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037342.01.T01 | YP_009656679.1 | 94.737 | 57 | 3 | 0 | 1 | 57 | 173 | 229 | 2.86E-28 | 110 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037342.01.T01 | P58155 | 91.228 | 57 | 5 | 0 | 1 | 57 | 173 | 229 | 1.35E-29 | 106 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037342.01.T01 | A0A4P8F2D4 | 94.737 | 57 | 3 | 0 | 1 | 57 | 173 | 229 | 1.36e-28 | 110 |
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsG0080047967.01 | MsG0780037342.01 | 0.807645 | 4.569329e-50 | 2.558680e-47 |
MsG0080048560.01 | MsG0780037342.01 | 0.845137 | 4.899949e-59 | 8.086303e-56 |
MsG0080048694.01 | MsG0780037342.01 | 0.813166 | 2.937597e-51 | 1.904048e-48 |
MsG0080048695.01 | MsG0780037342.01 | 0.813756 | 2.178824e-51 | 1.435170e-48 |
MsG0080048813.01 | MsG0780037342.01 | 0.817764 | 2.784333e-52 | 2.045957e-49 |
MsG0080049018.01 | MsG0780037342.01 | 0.805213 | 1.487886e-49 | 7.826308e-47 |
MsG0080049023.01 | MsG0780037342.01 | 0.800996 | 1.108858e-48 | 5.237199e-46 |
MsG0180000417.01 | MsG0780037342.01 | 0.814143 | 1.789939e-51 | 1.191463e-48 |
MsG0180000459.01 | MsG0780037342.01 | 0.808203 | 3.476317e-50 | 1.975514e-47 |
MsG0180000719.01 | MsG0780037342.01 | 0.810379 | 1.186851e-50 | 7.142805e-48 |
MsG0180001054.01 | MsG0780037342.01 | 0.806180 | 9.320864e-50 | 5.026203e-47 |
MsG0180001072.01 | MsG0780037342.01 | 0.824805 | 6.615993e-54 | 5.923020e-51 |
MsG0180002099.01 | MsG0780037342.01 | 0.803442 | 3.480149e-49 | 1.749001e-46 |
MsG0180004773.01 | MsG0780037342.01 | 0.808702 | 2.720093e-50 | 1.565983e-47 |
MsG0180005783.01 | MsG0780037342.01 | 0.807299 | 5.409021e-50 | 3.001944e-47 |
MsG0180005791.01 | MsG0780037342.01 | 0.828926 | 6.850246e-55 | 6.905837e-52 |
MsG0180006212.01 | MsG0780037342.01 | 0.800515 | 1.389775e-48 | 6.483903e-46 |
MsG0180006227.01 | MsG0780037342.01 | 0.813179 | 2.917185e-51 | 1.891559e-48 |
MsG0280010780.01 | MsG0780037342.01 | 0.802823 | 4.672304e-49 | 2.311459e-46 |
MsG0380013114.01 | MsG0780037342.01 | 0.814039 | 1.887462e-51 | 1.252858e-48 |
MsG0380013448.01 | MsG0780037342.01 | 0.818634 | 1.769691e-52 | 1.331810e-49 |
MsG0380013921.01 | MsG0780037342.01 | 0.816931 | 4.286737e-52 | 3.078891e-49 |
MsG0380013928.01 | MsG0780037342.01 | 0.818519 | 1.879145e-52 | 1.409798e-49 |
MsG0380013943.01 | MsG0780037342.01 | 0.801825 | 7.498279e-49 | 3.616212e-46 |
MsG0380013945.01 | MsG0780037342.01 | 0.802369 | 5.795828e-49 | 2.834260e-46 |
MsG0380013956.01 | MsG0780037342.01 | 0.814472 | 1.513836e-51 | 1.016774e-48 |
MsG0380013966.01 | MsG0780037342.01 | 0.802589 | 5.222423e-49 | 2.568041e-46 |
MsG0380013971.01 | MsG0780037342.01 | 0.813758 | 2.176890e-51 | 1.433950e-48 |
MsG0380013975.01 | MsG0780037342.01 | 0.801261 | 9.787533e-49 | 4.653389e-46 |
MsG0380013999.01 | MsG0780037342.01 | 0.800396 | 1.469871e-48 | 6.836860e-46 |
MsG0380014004.01 | MsG0780037342.01 | 0.810572 | 1.078536e-50 | 6.524345e-48 |
MsG0380014005.01 | MsG0780037342.01 | 0.816691 | 4.854273e-52 | 3.463337e-49 |
MsG0380014020.01 | MsG0780037342.01 | 0.822541 | 2.241934e-53 | 1.882130e-50 |
MsG0380014021.01 | MsG0780037342.01 | 0.816460 | 5.468407e-52 | 3.876906e-49 |
MsG0380014023.01 | MsG0780037342.01 | 0.824701 | 6.997293e-54 | 6.245850e-51 |
MsG0380014029.01 | MsG0780037342.01 | 0.817287 | 3.565327e-52 | 2.585678e-49 |
MsG0380014032.01 | MsG0780037342.01 | 0.810081 | 1.376454e-50 | 8.219500e-48 |
MsG0380014129.01 | MsG0780037342.01 | 0.821878 | 3.194505e-53 | 2.631534e-50 |
MsG0680031813.01 | MsG0780037342.01 | 0.809078 | 2.260049e-50 | 1.314259e-47 |
MsG0680034229.01 | MsG0780037342.01 | 0.803024 | 4.246628e-49 | 2.111379e-46 |
MsG0480018628.01 | MsG0780037342.01 | 0.815500 | 8.953468e-52 | 6.183902e-49 |
MsG0480020550.01 | MsG0780037342.01 | 0.804561 | 2.036155e-49 | 1.053164e-46 |
MsG0480021327.01 | MsG0780037342.01 | 0.833557 | 4.979466e-56 | 5.755089e-53 |
MsG0480021331.01 | MsG0780037342.01 | 0.838339 | 3.050431e-57 | 4.073198e-54 |
MsG0480021563.01 | MsG0780037342.01 | 0.800786 | 1.224133e-48 | 5.750189e-46 |
MsG0480022581.01 | MsG0780037342.01 | 0.808551 | 2.930163e-50 | 1.680350e-47 |
MsG0480022591.01 | MsG0780037342.01 | 0.820922 | 5.310776e-53 | 4.259143e-50 |
MsG0480023006.01 | MsG0780037342.01 | 0.809632 | 1.719241e-50 | 1.014719e-47 |
MsG0480023152.01 | MsG0780037342.01 | 0.815215 | 1.036078e-51 | 7.099195e-49 |
MsG0480023577.01 | MsG0780037342.01 | 0.802356 | 5.833704e-49 | 2.851785e-46 |
MsG0380015200.01 | MsG0780037342.01 | 0.809244 | 2.082103e-50 | 1.216216e-47 |
MsG0380015201.01 | MsG0780037342.01 | 0.819564 | 1.086964e-52 | 8.394143e-50 |
MsG0380015348.01 | MsG0780037342.01 | 0.826479 | 2.652178e-54 | 2.490068e-51 |
MsG0380016881.01 | MsG0780037342.01 | 0.811822 | 5.776060e-51 | 3.611871e-48 |
MsG0380017405.01 | MsG0780037342.01 | 0.831694 | 1.443526e-55 | 1.578512e-52 |
MsG0380017406.01 | MsG0780037342.01 | 0.807607 | 4.653835e-50 | 2.603434e-47 |
MsG0380017812.01 | MsG0780037342.01 | 0.820912 | 5.337208e-53 | 4.279195e-50 |
MsG0480018088.01 | MsG0780037342.01 | 0.805024 | 1.630059e-49 | 8.532392e-47 |
MsG0480018296.01 | MsG0780037342.01 | 0.812744 | 3.634839e-51 | 2.329788e-48 |
MsG0580027677.01 | MsG0780037342.01 | 0.800173 | 1.631972e-48 | 7.547695e-46 |
MsG0580029820.01 | MsG0780037342.01 | 0.803012 | 4.271051e-49 | 2.122831e-46 |
MsG0680030439.01 | MsG0780037342.01 | 0.813368 | 2.651827e-51 | 1.728514e-48 |
MsG0680030644.01 | MsG0780037342.01 | 0.824955 | 6.098447e-54 | 5.482983e-51 |
MsG0280009094.01 | MsG0780037342.01 | 0.810036 | 1.407212e-50 | 8.394048e-48 |
MsG0280009742.01 | MsG0780037342.01 | 0.804425 | 2.173330e-49 | 1.120168e-46 |
MsG0280009864.01 | MsG0780037342.01 | 0.804631 | 1.968505e-49 | 1.020003e-46 |
MsG0280010142.01 | MsG0780037342.01 | 0.810441 | 1.150932e-50 | 6.938059e-48 |
MsG0280010227.01 | MsG0780037342.01 | 0.801854 | 7.397821e-49 | 3.570422e-46 |
MsG0780036002.01 | MsG0780037342.01 | 0.842246 | 2.906747e-58 | 4.380653e-55 |
MsG0780036004.01 | MsG0780037342.01 | 0.814877 | 1.231624e-51 | 8.362149e-49 |
MsG0780037342.01 | MsG0780037458.01 | 0.809982 | 1.445758e-50 | 8.611577e-48 |
MsG0780037342.01 | MsG0780038030.01 | 0.823346 | 1.455749e-53 | 1.250093e-50 |
MsG0780037342.01 | MsG0780038047.01 | 0.817626 | 2.991189e-52 | 2.189553e-49 |
MsG0780037342.01 | MsG0780038108.01 | 0.817515 | 3.168140e-52 | 2.312180e-49 |
MsG0780037342.01 | MsG0780038197.01 | 0.852027 | 6.042424e-61 | 1.246127e-57 |
MsG0780037342.01 | MsG0780038263.01 | 0.811834 | 5.741128e-51 | 3.591137e-48 |
MsG0780037342.01 | MsG0780039836.01 | 0.813466 | 2.523611e-51 | 1.649365e-48 |
MsG0780037342.01 | MsG0780039838.01 | 0.819382 | 1.195909e-52 | 9.187916e-50 |
MsG0780037342.01 | MsG0780039839.01 | 0.819954 | 8.855065e-53 | 6.913199e-50 |
MsG0780037342.01 | MsG0780040321.01 | 0.818190 | 2.231201e-52 | 1.658718e-49 |
MsG0780037342.01 | MsG0780041121.01 | 0.830880 | 2.289096e-55 | 2.443321e-52 |
MsG0780037342.01 | MsG0780041783.01 | 0.804611 | 1.988120e-49 | 1.029654e-46 |
MsG0780037342.01 | MsG0880042291.01 | 0.817641 | 2.968252e-52 | 2.173654e-49 |
MsG0780037342.01 | MsG0880042858.01 | 0.814973 | 1.172688e-51 | 7.982985e-49 |
MsG0780037342.01 | MsG0880045273.01 | 0.830755 | 2.456236e-55 | 2.612096e-52 |
MsG0780037342.01 | MsG0880045274.01 | 0.805306 | 1.422467e-49 | 7.500020e-47 |
MsG0780037342.01 | MsG0880045321.01 | 0.830630 | 2.635657e-55 | 2.792502e-52 |
MsG0780037342.01 | MsG0880045369.01 | 0.816921 | 4.310533e-52 | 3.095008e-49 |
MsG0780037342.01 | MsG0880045385.01 | 0.821079 | 4.884194e-53 | 3.934475e-50 |
MsG0780037342.01 | MsG0880045630.01 | 0.843326 | 1.501198e-58 | 2.340769e-55 |
MsG0780037342.01 | MsG0880045755.01 | 0.809569 | 1.773192e-50 | 1.044769e-47 |
MsG0780037342.01 | MsG0880046189.01 | 0.811562 | 6.578342e-51 | 4.085415e-48 |
MsG0780037342.01 | MsG0880046469.01 | 0.826008 | 3.433490e-54 | 3.180955e-51 |
MsG0780037342.01 | MsG0880046630.01 | 0.814201 | 1.738323e-51 | 1.158878e-48 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037342.01.T01 | ATCG00530 | 77.193 | 57 | 13 | 0 | 1 | 57 | 173 | 229 | 2.76e-25 | 93.2 |
Find 8 sgRNAs with CRISPR-Local
Find 7 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GGGTCTGTCTATAAATATTT+TGG | 0.174437 | 7:+23968676 | MsG0780037342.01.T01:CDS |
CATGGTTGGGAACTAATGAT+TGG | 0.443529 | 7:+23968655 | None:intergenic |
AAAATTGTATCTAAAATGAC+TGG | 0.459050 | 7:-23968745 | None:intergenic |
ATCTAAAATGACTGGAAAAG+TGG | 0.472631 | 7:-23968737 | None:intergenic |
CAATGAATGACTATAGCTCT+TGG | 0.495815 | 7:+23968830 | MsG0780037342.01.T01:CDS |
GAGATACACGATTTGAATAA+CGG | 0.513932 | 7:-23968783 | None:intergenic |
ATGGTTGGGAACTAATGATT+GGG | 0.515648 | 7:+23968656 | None:intergenic |
TGATAAATCACTACAAGTGA+CGG | 0.591823 | 7:-23968805 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!!! | TAGATACAATTTTAAAATAC+TGG | + | Chr7:23968755-23968774 | MsG0780037342.01.T01:CDS | 15.0% |
!!! | AAAATTGTATCTAAAATGAC+TGG | - | Chr7:23968748-23968767 | None:intergenic | 20.0% |
GAGATACACGATTTGAATAA+CGG | - | Chr7:23968786-23968805 | None:intergenic | 30.0% | |
TGATAAATCACTACAAGTGA+CGG | - | Chr7:23968808-23968827 | None:intergenic | 30.0% | |
! | ATCTAAAATGACTGGAAAAG+TGG | - | Chr7:23968740-23968759 | None:intergenic | 30.0% |
! | GGGTCTGTCTATAAATATTT+TGG | + | Chr7:23968676-23968695 | MsG0780037342.01.T01:CDS | 30.0% |
CAATGAATGACTATAGCTCT+TGG | + | Chr7:23968830-23968849 | MsG0780037342.01.T01:CDS | 35.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr7 | gene | 23968670 | 23968855 | 23968670 | ID=MsG0780037342.01;Name=MsG0780037342.01 |
Chr7 | mRNA | 23968670 | 23968855 | 23968670 | ID=MsG0780037342.01.T01;Parent=MsG0780037342.01;Name=MsG0780037342.01.T01;_AED=0.38;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|61 |
Chr7 | exon | 23968670 | 23968855 | 23968670 | ID=MsG0780037342.01.T01:exon:31994;Parent=MsG0780037342.01.T01 |
Chr7 | CDS | 23968670 | 23968855 | 23968670 | ID=MsG0780037342.01.T01:cds;Parent=MsG0780037342.01.T01 |
Chr7 | mRNA | 23968710 | 23968757 | 23968710 | ID=MsG0780037342.01.T02;Parent=MsG0780037342.01;Name=MsG0780037342.01.T02;_AED=0.47;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|15 |
Chr7 | exon | 23968710 | 23968757 | 23968710 | ID=MsG0780037342.01.T02:exon:31995;Parent=MsG0780037342.01.T02 |
Chr7 | CDS | 23968710 | 23968757 | 23968710 | ID=MsG0780037342.01.T02:cds;Parent=MsG0780037342.01.T02 |
Chr7 | mRNA | 23968832 | 23968855 | 23968832 | ID=MsG0780037342.01.T03;Parent=MsG0780037342.01;Name=MsG0780037342.01.T03;_AED=0.48;_eAED=0.81;_QI=0|-1|0|1|-1|0|1|0|7 |
Chr7 | exon | 23968832 | 23968855 | 23968832 | ID=MsG0780037342.01.T03:exon:31996;Parent=MsG0780037342.01.T03 |
Chr7 | CDS | 23968832 | 23968855 | 23968832 | ID=MsG0780037342.01.T03:cds;Parent=MsG0780037342.01.T03 |
Gene Sequence |
Protein sequence |