AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037342.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037342.01.T01 ATCG00530 77.193 57 13 0 1 57 173 229 2.76e-25 93.2

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTCTGTCTATAAATATTT+TGG 0.174437 7:+23968676 MsG0780037342.01.T01:CDS
CATGGTTGGGAACTAATGAT+TGG 0.443529 7:+23968655 None:intergenic
AAAATTGTATCTAAAATGAC+TGG 0.459050 7:-23968745 None:intergenic
ATCTAAAATGACTGGAAAAG+TGG 0.472631 7:-23968737 None:intergenic
CAATGAATGACTATAGCTCT+TGG 0.495815 7:+23968830 MsG0780037342.01.T01:CDS
GAGATACACGATTTGAATAA+CGG 0.513932 7:-23968783 None:intergenic
ATGGTTGGGAACTAATGATT+GGG 0.515648 7:+23968656 None:intergenic
TGATAAATCACTACAAGTGA+CGG 0.591823 7:-23968805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGATACAATTTTAAAATAC+TGG + Chr7:23968755-23968774 MsG0780037342.01.T01:CDS 15.0%
!!! AAAATTGTATCTAAAATGAC+TGG - Chr7:23968748-23968767 None:intergenic 20.0%
GAGATACACGATTTGAATAA+CGG - Chr7:23968786-23968805 None:intergenic 30.0%
TGATAAATCACTACAAGTGA+CGG - Chr7:23968808-23968827 None:intergenic 30.0%
! ATCTAAAATGACTGGAAAAG+TGG - Chr7:23968740-23968759 None:intergenic 30.0%
! GGGTCTGTCTATAAATATTT+TGG + Chr7:23968676-23968695 MsG0780037342.01.T01:CDS 30.0%
CAATGAATGACTATAGCTCT+TGG + Chr7:23968830-23968849 MsG0780037342.01.T01:CDS 35.0%
Chromosome Type Strat End Strand Name
Chr7 gene 23968670 23968855 23968670 ID=MsG0780037342.01;Name=MsG0780037342.01
Chr7 mRNA 23968670 23968855 23968670 ID=MsG0780037342.01.T01;Parent=MsG0780037342.01;Name=MsG0780037342.01.T01;_AED=0.38;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|61
Chr7 exon 23968670 23968855 23968670 ID=MsG0780037342.01.T01:exon:31994;Parent=MsG0780037342.01.T01
Chr7 CDS 23968670 23968855 23968670 ID=MsG0780037342.01.T01:cds;Parent=MsG0780037342.01.T01
Chr7 mRNA 23968710 23968757 23968710 ID=MsG0780037342.01.T02;Parent=MsG0780037342.01;Name=MsG0780037342.01.T02;_AED=0.47;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|15
Chr7 exon 23968710 23968757 23968710 ID=MsG0780037342.01.T02:exon:31995;Parent=MsG0780037342.01.T02
Chr7 CDS 23968710 23968757 23968710 ID=MsG0780037342.01.T02:cds;Parent=MsG0780037342.01.T02
Chr7 mRNA 23968832 23968855 23968832 ID=MsG0780037342.01.T03;Parent=MsG0780037342.01;Name=MsG0780037342.01.T03;_AED=0.48;_eAED=0.81;_QI=0|-1|0|1|-1|0|1|0|7
Chr7 exon 23968832 23968855 23968832 ID=MsG0780037342.01.T03:exon:31996;Parent=MsG0780037342.01.T03
Chr7 CDS 23968832 23968855 23968832 ID=MsG0780037342.01.T03:cds;Parent=MsG0780037342.01.T03
Gene Sequence

>MsG0780037342.01.T02

ATGATCAAATTATATCTAGTCTTGTTTCCACTTTTCCAGTCATTTTAG

>MsG0780037342.01.T03

ATGAATGACTATAGCTCTTGGTAA

>MsG0780037342.01.T01

ATGATTGGGTCTGTCTATAAATATTTTGGATTTACTCAGAATGATCAAATTATATCTAGTCTTGTTTCCACTTTTCCAGTCATTTTAGATACAATTTTAAAATACTGGATTTTCCGTTATTCAAATCGTGTATCTCCGTCACTTGTAGTGATTTATCATTCAATGAATGACTATAGCTCTTGGTAA

Protein sequence

>MsG0780037342.01.T02

MIKLYLVLFPLFQSF*

>MsG0780037342.01.T03

MNDYSSW*

>MsG0780037342.01.T01

MIGSVYKYFGFTQNDQIISSLVSTFPVILDTILKYWIFRYSNRVSPSLVVIYHSMNDYSSW*