AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045273.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045273.01.T01 MTR_4g050770 93.269 104 7 0 1 104 1 104 1.97e-65 194
MsG0880045273.01.T01 MTR_2g089580 92.308 104 8 0 1 104 1 104 4.50e-64 190
MsG0880045273.01.T01 MTR_4g051290 93.269 104 7 0 1 104 1 104 1.24e-63 196
MsG0880045273.01.T01 MTR_7g021750 92.308 104 8 0 1 104 1 104 5.17e-63 195
MsG0880045273.01.T01 MTR_0002s0380 91.346 104 9 0 1 104 1 104 2.57e-62 193
MsG0880045273.01.T01 MTR_1g057280 82.178 101 18 0 4 104 1 101 2.43e-56 171
MsG0880045273.01.T01 MTR_4g006190 83.824 68 11 0 37 104 1 68 4.38e-32 108
MsG0880045273.01.T01 MTR_7g017540 77.193 57 13 0 37 93 1 57 1.09e-21 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045273.01.T01 ATCG00020 92.308 104 8 0 1 104 1 104 3.83e-63 195

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATTCATGAGCCTGTTTC+TGG 0.177223 8:+56760256 MsG0880045273.01.T01:CDS
TATGGGGTCGCTTCTGTAAC+TGG 0.325492 8:+56760110 MsG0880045273.01.T01:CDS
TACAGAAGTTGCTGTCAATA+AGG 0.343087 8:-56760191 None:intergenic
ACGGATGCAGCTTCCCATAT+CGG 0.367380 8:-56760355 None:intergenic
CGTCTTTACATTGGATGGTT+TGG 0.372317 8:+56760151 MsG0880045273.01.T01:CDS
GACGCGATAGCGAAAACCTA+TGG 0.411483 8:+56760092 MsG0880045273.01.T01:CDS
AGCTGGAGAAGTAGGAATAA+TGG 0.420240 8:-56760314 None:intergenic
TAAAGTAGAGATCCAGAAAC+AGG 0.424664 8:-56760268 None:intergenic
TGAATACCATCAATATCTAC+TGG 0.449872 8:-56760241 None:intergenic
CGGATGCAGCTTCCCATATC+GGG 0.477757 8:-56760354 None:intergenic
GCCCCTCCAGTAGATATTGA+TGG 0.479743 8:+56760235 MsG0880045273.01.T01:CDS
ACGCGATAGCGAAAACCTAT+GGG 0.487730 8:+56760093 MsG0880045273.01.T01:CDS
GAAGTTGCTGTCAATAAGGT+AGG 0.500574 8:-56760187 None:intergenic
TAAAAGTGCAAACTGATAGC+TGG 0.505767 8:-56760331 None:intergenic
AAAACCGTCTTTACATTGGA+TGG 0.510740 8:+56760146 MsG0880045273.01.T01:CDS
ATTGAAAACCGTCTTTACAT+TGG 0.542553 8:+56760142 MsG0880045273.01.T01:CDS
AAACTGATAGCTGGAGAAGT+AGG 0.552926 8:-56760322 None:intergenic
TCTCTCTAAAATTGCAGTCA+TGG 0.558841 8:-56760071 None:intergenic
TACCATCAATATCTACTGGA+GGG 0.569112 8:-56760237 None:intergenic
ACCATCAATATCTACTGGAG+GGG 0.588944 8:-56760236 None:intergenic
AAGTTGCTGTCAATAAGGTA+GGG 0.608775 8:-56760186 None:intergenic
AGTTACAGAAGCGACCCCAT+AGG 0.631772 8:-56760108 None:intergenic
GTTGTATAATCATTCATCAA+CGG 0.635546 8:-56760374 None:intergenic
CAAACCATCCAATGTAAAGA+CGG 0.643551 8:-56760150 None:intergenic
CGCGATAGCGAAAACCTATG+GGG 0.644401 8:+56760094 MsG0880045273.01.T01:CDS
ATACCATCAATATCTACTGG+AGG 0.649917 8:-56760238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTGAAAACCGTCTTTACAT+TGG + Chr8:56760142-56760161 MsG0880045273.01.T01:CDS 30.0%
TGAATACCATCAATATCTAC+TGG - Chr8:56760244-56760263 None:intergenic 30.0%
AAAACCGTCTTTACATTGGA+TGG + Chr8:56760146-56760165 MsG0880045273.01.T01:CDS 35.0%
AAGTTGCTGTCAATAAGGTA+GGG - Chr8:56760189-56760208 None:intergenic 35.0%
ATACCATCAATATCTACTGG+AGG - Chr8:56760241-56760260 None:intergenic 35.0%
CAAACCATCCAATGTAAAGA+CGG - Chr8:56760153-56760172 None:intergenic 35.0%
TAAAGTAGAGATCCAGAAAC+AGG - Chr8:56760271-56760290 None:intergenic 35.0%
TACAGAAGTTGCTGTCAATA+AGG - Chr8:56760194-56760213 None:intergenic 35.0%
TACCATCAATATCTACTGGA+GGG - Chr8:56760240-56760259 None:intergenic 35.0%
! TAAAAGTGCAAACTGATAGC+TGG - Chr8:56760334-56760353 None:intergenic 35.0%
! TTTGCACTTTTACCCGATAT+GGG + Chr8:56760342-56760361 MsG0880045273.01.T01:CDS 35.0%
AAACTGATAGCTGGAGAAGT+AGG - Chr8:56760325-56760344 None:intergenic 40.0%
ACCATCAATATCTACTGGAG+GGG - Chr8:56760239-56760258 None:intergenic 40.0%
AGCTGGAGAAGTAGGAATAA+TGG - Chr8:56760317-56760336 None:intergenic 40.0%
CGTCTTTACATTGGATGGTT+TGG + Chr8:56760151-56760170 MsG0880045273.01.T01:CDS 40.0%
GAAGTTGCTGTCAATAAGGT+AGG - Chr8:56760190-56760209 None:intergenic 40.0%
! GTAAAGACGGTTTTCAATGC+TGG - Chr8:56760140-56760159 None:intergenic 40.0%
! GTTTGCACTTTTACCCGATA+TGG + Chr8:56760341-56760360 MsG0880045273.01.T01:CDS 40.0%
ACGCGATAGCGAAAACCTAT+GGG + Chr8:56760093-56760112 MsG0880045273.01.T01:CDS 45.0%
GGTATTCATGAGCCTGTTTC+TGG + Chr8:56760256-56760275 MsG0880045273.01.T01:CDS 45.0%
ACGGATGCAGCTTCCCATAT+CGG - Chr8:56760358-56760377 None:intergenic 50.0%
AGTTACAGAAGCGACCCCAT+AGG - Chr8:56760111-56760130 None:intergenic 50.0%
CGCGATAGCGAAAACCTATG+GGG + Chr8:56760094-56760113 MsG0880045273.01.T01:CDS 50.0%
GACGCGATAGCGAAAACCTA+TGG + Chr8:56760092-56760111 MsG0880045273.01.T01:CDS 50.0%
GCCCCTCCAGTAGATATTGA+TGG + Chr8:56760235-56760254 MsG0880045273.01.T01:CDS 50.0%
TATGGGGTCGCTTCTGTAAC+TGG + Chr8:56760110-56760129 MsG0880045273.01.T01:CDS 50.0%
CGGATGCAGCTTCCCATATC+GGG - Chr8:56760357-56760376 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 56760073 56760387 56760073 ID=MsG0880045273.01;Name=MsG0880045273.01
Chr8 mRNA 56760073 56760387 56760073 ID=MsG0880045273.01.T01;Parent=MsG0880045273.01;Name=MsG0880045273.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|104
Chr8 exon 56760073 56760387 56760073 ID=MsG0880045273.01.T01:exon:1159;Parent=MsG0880045273.01.T01
Chr8 CDS 56760073 56760387 56760073 ID=MsG0880045273.01.T01:cds;Parent=MsG0880045273.01.T01
Gene Sequence

>MsG0880045273.01.T01

ATGACTGCAATTTTAGAGAGACGCGATAGCGAAAACCTATGGGGTCGCTTCTGTAACTGGATAACCAGCATTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACAGCAACTTCTGTATTTATTATCGCTTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCATGAGCCTGTTTCTGGATCTCTACTTTACGAAAACAATATTATTTCTAGTGCCATTATTCCTACTTCTCCAGCTATCAGTTTGCACTTTTACCCGATATGGGAAGCTGCATCCGTTGATGAATGA

Protein sequence

>MsG0880045273.01.T01

MTAILERRDSENLWGRFCNWITSIENRLYIGWFGVLMIPTLLTATSVFIIAFIAAPPVDIDGIHEPVSGSLLYENNIISSAIIPTSPAISLHFYPIWEAASVDE*