AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023577.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023577.01.T01 MTR_8g096310 60.694 173 30 5 9 150 29 194 4.66e-51 164
MsG0480023577.01.T01 MTR_3g098910 37.569 181 74 5 2 150 21 194 1.73e-26 101
MsG0480023577.01.T01 MTR_7g405730 34.969 163 79 6 9 146 29 189 3.84e-19 81.6
MsG0480023577.01.T01 MTR_7g405710 32.530 166 75 6 11 146 31 189 3.85e-16 73.6
MsG0480023577.01.T01 MTR_5g067530 34.756 164 74 6 11 146 32 190 5.62e-16 73.2
MsG0480023577.01.T01 MTR_8g096320 32.934 167 86 5 11 152 31 196 6.25e-15 70.1
MsG0480023577.01.T01 MTR_3g098930 32.386 176 80 7 11 155 32 199 5.58e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023577.01.T01 AT5G23660 41.975 162 69 5 11 148 33 193 7.68e-26 99.8
MsG0480023577.01.T01 AT3G48740 38.690 168 66 6 11 148 33 193 4.68e-22 89.7
MsG0480023577.01.T01 AT5G50800 37.725 167 74 6 11 150 31 194 6.27e-22 89.4
MsG0480023577.01.T01 AT4G25010 34.132 167 84 5 11 152 31 196 1.55e-18 80.1
MsG0480023577.01.T01 AT5G50790 27.528 178 86 6 2 146 22 189 2.09e-14 68.9
MsG0480023577.01.T01 AT5G13170 30.539 167 78 6 11 146 33 192 8.47e-14 67.4

Find 28 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGATTGGGTTGTTTAATT+TGG 0.148249 4:+87181690 MsG0480023577.01.T01:CDS
ATTACATACCCTTGCTTTCT+TGG 0.188827 4:-87181440 None:intergenic
CTGATTGGGTTGTTTAATTT+GGG 0.255905 4:+87181691 MsG0480023577.01.T01:CDS
TCAACAATTTATAGTAGAAT+TGG 0.284348 4:-87182058 None:intergenic
CCACAACAAGTGCAATTATA+TGG 0.333131 4:+87181987 MsG0480023577.01.T01:CDS
AGGCATAAACTCTACACTTT+TGG 0.335245 4:-87181946 None:intergenic
ATCTTCACATTGAAGCTGAT+TGG 0.360466 4:+87181676 MsG0480023577.01.T01:CDS
CACTCTTCAGTGCAATGCTT+TGG 0.375576 4:+87181315 MsG0480023577.01.T01:CDS
TCACACAACCAATGCATTGA+TGG 0.392438 4:-87181379 None:intergenic
TCTTCACATTGAAGCTGATT+GGG 0.409416 4:+87181677 MsG0480023577.01.T01:CDS
CCTCAAAGATATATTTGTTA+CGG 0.420795 4:+87182027 MsG0480023577.01.T01:CDS
CTGAAGAGTGCAGCCACATA+TGG 0.434896 4:-87181302 None:intergenic
CCGTAACAAATATATCTTTG+AGG 0.464322 4:-87182027 None:intergenic
CCATATAATTGCACTTGTTG+TGG 0.499417 4:-87181987 None:intergenic
GTTTGTAAGAAGAAAACAAC+AGG 0.503909 4:+87181263 MsG0480023577.01.T01:CDS
CAGCCACATATGGAGCAGAT+TGG 0.522606 4:-87181292 None:intergenic
CTTATTGCTCCAAGAAAGCA+AGG 0.525447 4:+87181431 MsG0480023577.01.T01:CDS
GGAGCAATAAGTGATATAGA+TGG 0.527394 4:-87181419 None:intergenic
TATCATCACCATCAATGCAT+TGG 0.530268 4:+87181371 MsG0480023577.01.T01:CDS
TTTCCAATCTGCTCCATATG+TGG 0.537778 4:+87181289 MsG0480023577.01.T01:CDS
ATTGGGTTGTTTAATTTGGG+AGG 0.542194 4:+87181694 MsG0480023577.01.T01:CDS
ATAATTGCACTTGTTGTGGA+AGG 0.570675 4:-87181983 None:intergenic
GGAAGGAGTGAAAGAGTGAA+AGG 0.571621 4:-87181966 None:intergenic
TAATTTGGGAGGAATTTGCT+TGG 0.577927 4:+87181705 MsG0480023577.01.T01:CDS
GCAATTATATGGCTGTGCTA+TGG 0.628621 4:+87181998 MsG0480023577.01.T01:CDS
TGTAAGAAGAAAACAACAGG+AGG 0.632520 4:+87181266 MsG0480023577.01.T01:CDS
AATAAGTGATATAGATGGCG+AGG 0.639838 4:-87181414 None:intergenic
TTATTGCTCCAAGAAAGCAA+GGG 0.730949 4:+87181432 MsG0480023577.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTAATTATTATTAGT+AGG + Chr4:87181609-87181628 MsG0480023577.01.T01:intron 10.0%
!! TATACTACTAAAAAAAATTA+TGG + Chr4:87181488-87181507 MsG0480023577.01.T01:intron 10.0%
!!! TATTTTATGCCTACATAAAA+CGG + Chr4:87181339-87181358 MsG0480023577.01.T01:CDS 20.0%
!! ATTTTATGCCTACATAAAAC+GGG + Chr4:87181340-87181359 MsG0480023577.01.T01:CDS 25.0%
!! CCTCAAAGATATATTTGTTA+CGG + Chr4:87182027-87182046 MsG0480023577.01.T01:CDS 25.0%
CCGTAACAAATATATCTTTG+AGG - Chr4:87182030-87182049 None:intergenic 30.0%
GTTTGTAAGAAGAAAACAAC+AGG + Chr4:87181263-87181282 MsG0480023577.01.T01:CDS 30.0%
! ATTTGAGACTTGTGTGTTAT+TGG + Chr4:87181645-87181664 MsG0480023577.01.T01:intron 30.0%
! CTGATTGGGTTGTTTAATTT+GGG + Chr4:87181691-87181710 MsG0480023577.01.T01:CDS 30.0%
AATAAGTGATATAGATGGCG+AGG - Chr4:87181417-87181436 None:intergenic 35.0%
ATACTCACCATAACGCTTAA+AGG - Chr4:87181829-87181848 None:intergenic 35.0%
ATCTTCACATTGAAGCTGAT+TGG + Chr4:87181676-87181695 MsG0480023577.01.T01:CDS 35.0%
ATTACATACCCTTGCTTTCT+TGG - Chr4:87181443-87181462 None:intergenic 35.0%
CCACAACAAGTGCAATTATA+TGG + Chr4:87181987-87182006 MsG0480023577.01.T01:CDS 35.0%
GGAGCAATAAGTGATATAGA+TGG - Chr4:87181422-87181441 None:intergenic 35.0%
TATCATCAAGTTAGTGAACG+AGG + Chr4:87181548-87181567 MsG0480023577.01.T01:intron 35.0%
TATCATCACCATCAATGCAT+TGG + Chr4:87181371-87181390 MsG0480023577.01.T01:CDS 35.0%
TCTTCACATTGAAGCTGATT+GGG + Chr4:87181677-87181696 MsG0480023577.01.T01:CDS 35.0%
TGAAATTGAACACGGTGATT+TGG - Chr4:87181909-87181928 None:intergenic 35.0%
TGTAAGAAGAAAACAACAGG+AGG + Chr4:87181266-87181285 MsG0480023577.01.T01:CDS 35.0%
TTATTGCTCCAAGAAAGCAA+GGG + Chr4:87181432-87181451 MsG0480023577.01.T01:CDS 35.0%
! AGGCATAAACTCTACACTTT+TGG - Chr4:87181949-87181968 None:intergenic 35.0%
! CCATATAATTGCACTTGTTG+TGG - Chr4:87181990-87182009 None:intergenic 35.0%
! GCTGATTGGGTTGTTTAATT+TGG + Chr4:87181690-87181709 MsG0480023577.01.T01:CDS 35.0%
! TAATTTGGGAGGAATTTGCT+TGG + Chr4:87181705-87181724 MsG0480023577.01.T01:CDS 35.0%
!! ATAATTGCACTTGTTGTGGA+AGG - Chr4:87181986-87182005 None:intergenic 35.0%
!! ATTGGGTTGTTTAATTTGGG+AGG + Chr4:87181694-87181713 MsG0480023577.01.T01:CDS 35.0%
AACGTATCGAACTTCTTGGA+TGG + Chr4:87181767-87181786 MsG0480023577.01.T01:intron 40.0%
ACAGAACGTATCGAACTTCT+TGG + Chr4:87181763-87181782 MsG0480023577.01.T01:intron 40.0%
CTGCAAAAACACTAGTTGAG+AGG - Chr4:87181804-87181823 None:intergenic 40.0%
CTTATTGCTCCAAGAAAGCA+AGG + Chr4:87181431-87181450 MsG0480023577.01.T01:CDS 40.0%
GCAATTATATGGCTGTGCTA+TGG + Chr4:87181998-87182017 MsG0480023577.01.T01:CDS 40.0%
TCACACAACCAATGCATTGA+TGG - Chr4:87181382-87181401 None:intergenic 40.0%
TTTCCAATCTGCTCCATATG+TGG + Chr4:87181289-87181308 MsG0480023577.01.T01:CDS 40.0%
! AGCATTCACCCGTTTTATGT+AGG - Chr4:87181351-87181370 None:intergenic 40.0%
!! CCACTCTCTGAAATTGAACA+CGG - Chr4:87181917-87181936 None:intergenic 40.0%
CACTCTTCAGTGCAATGCTT+TGG + Chr4:87181315-87181334 MsG0480023577.01.T01:CDS 45.0%
CCGTGTTCAATTTCAGAGAG+TGG + Chr4:87181914-87181933 MsG0480023577.01.T01:intron 45.0%
GGAAGGAGTGAAAGAGTGAA+AGG - Chr4:87181969-87181988 None:intergenic 45.0%
TGCAGCACCTTTAAGCGTTA+TGG + Chr4:87181819-87181838 MsG0480023577.01.T01:intron 45.0%
CAGCCACATATGGAGCAGAT+TGG - Chr4:87181295-87181314 None:intergenic 50.0%
! CTGAAGAGTGCAGCCACATA+TGG - Chr4:87181305-87181324 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 87181224 87182072 87181224 ID=MsG0480023577.01;Name=MsG0480023577.01
Chr4 mRNA 87181224 87182072 87181224 ID=MsG0480023577.01.T01;Parent=MsG0480023577.01;Name=MsG0480023577.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|0|0|3|0|156
Chr4 exon 87181224 87181457 87181224 ID=MsG0480023577.01.T01:exon:6541;Parent=MsG0480023577.01.T01
Chr4 exon 87181676 87181769 87181676 ID=MsG0480023577.01.T01:exon:6542;Parent=MsG0480023577.01.T01
Chr4 exon 87181930 87182072 87181930 ID=MsG0480023577.01.T01:exon:6543;Parent=MsG0480023577.01.T01
Chr4 CDS 87181224 87181457 87181224 ID=MsG0480023577.01.T01:cds;Parent=MsG0480023577.01.T01
Chr4 CDS 87181676 87181769 87181676 ID=MsG0480023577.01.T01:cds;Parent=MsG0480023577.01.T01
Chr4 CDS 87181930 87182072 87181930 ID=MsG0480023577.01.T01:cds;Parent=MsG0480023577.01.T01
Gene Sequence

>MsG0480023577.01.T01

ATGTATATTTGTATACTTTGCAGATCAATATTTTATCAAGTTTGTAAGAAGAAAACAACAGGAGGTTTCCAATCTGCTCCATATGTGGCTGCACTCTTCAGTGCAATGCTTTGGATATTTTATGCCTACATAAAACGGGTGAATGCTTATCATCACCATCAATGCATTGGTTGTGTGATAGAAACAATTACCTCGCCATCTATATCACTTATTGCTCCAAGAAAGCAAGGGTATATCTTCACATTGAAGCTGATTGGGTTGTTTAATTTGGGAGGAATTTGCTTGGTGATTATTCTAACACATGTTCTAGCAAAAGAACGAACAGAACAGAGTGGTTATTCGCACCAAAAGTGTAGAGTTTATGCCTTTCACTCTTTCACTCCTTCCACAACAAGTGCAATTATATGGCTGTGCTATGGTATTCTCCTCAAAGATATATTTGTTACGGTAAGTACTCCAATTCTACTATAA

Protein sequence

>MsG0480023577.01.T01

MYICILCRSIFYQVCKKKTTGGFQSAPYVAALFSAMLWIFYAYIKRVNAYHHHQCIGCVIETITSPSISLIAPRKQGYIFTLKLIGLFNLGGICLVIILTHVLAKERTEQSGYSHQKCRVYAFHSFTPSTTSAIIWLCYGILLKDIFVTVSTPILL*