Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043915.01.T01 | XP_003554698.1 | 55.643 | 381 | 103 | 6 | 1 | 318 | 1 | 378 | 3.86E-121 | 363 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043915.01.T01 | I1NC72 | 55.643 | 381 | 103 | 6 | 1 | 318 | 1 | 378 | 1.84e-121 | 363 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048887.01 | MsG0880043915.01 | 0.829850 | 4.085742e-55 | 4.233004e-52 |
| MsG0080048918.01 | MsG0880043915.01 | 0.837091 | 6.378970e-57 | 8.197776e-54 |
| MsG0080048919.01 | MsG0880043915.01 | 0.846788 | 1.742520e-59 | 3.033494e-56 |
| MsG0080049024.01 | MsG0880043915.01 | 0.842064 | 3.248029e-58 | 4.866980e-55 |
| MsG0080049066.01 | MsG0880043915.01 | 0.822321 | 2.521936e-53 | 2.103893e-50 |
| MsG0180000467.01 | MsG0880043915.01 | 0.801724 | 7.867785e-49 | 3.784449e-46 |
| MsG0180001137.01 | MsG0880043915.01 | 0.845126 | 4.931879e-59 | 8.136390e-56 |
| MsG0180001864.01 | MsG0880043915.01 | 0.803571 | 3.271286e-49 | 1.649628e-46 |
| MsG0180003351.01 | MsG0880043915.01 | 0.813715 | 2.224506e-51 | 1.463699e-48 |
| MsG0180003405.01 | MsG0880043915.01 | 0.815327 | 9.784981e-52 | 6.725774e-49 |
| MsG0180003617.01 | MsG0880043915.01 | 0.800851 | 1.187181e-48 | 5.586205e-46 |
| MsG0180003758.01 | MsG0880043915.01 | 0.834328 | 3.194358e-56 | 3.778662e-53 |
| MsG0180004581.01 | MsG0880043915.01 | 0.800571 | 1.353827e-48 | 6.325082e-46 |
| MsG0180004714.01 | MsG0880043915.01 | 0.845200 | 4.709895e-59 | 7.789071e-56 |
| MsG0180004737.01 | MsG0880043915.01 | 0.801754 | 7.756221e-49 | 3.733658e-46 |
| MsG0180004787.01 | MsG0880043915.01 | 0.825354 | 4.908082e-54 | 4.462814e-51 |
| MsG0180005369.01 | MsG0880043915.01 | 0.823777 | 1.153967e-53 | 1.003286e-50 |
| MsG0180005666.01 | MsG0880043915.01 | 0.816719 | 4.784297e-52 | 3.416187e-49 |
| MsG0180005898.01 | MsG0880043915.01 | 0.824658 | 7.162525e-54 | 6.385483e-51 |
| MsG0780040214.01 | MsG0880043915.01 | 0.823364 | 1.441619e-53 | 1.238558e-50 |
| MsG0780040236.01 | MsG0880043915.01 | 0.821546 | 3.811619e-53 | 3.110431e-50 |
| MsG0780040640.01 | MsG0880043915.01 | 0.823634 | 1.246010e-53 | 1.078887e-50 |
| MsG0780040685.01 | MsG0880043915.01 | 0.842463 | 2.546116e-58 | 3.863875e-55 |
| MsG0780040892.01 | MsG0880043915.01 | 0.824468 | 7.940439e-54 | 7.039023e-51 |
| MsG0780041625.01 | MsG0880043915.01 | 0.906686 | 1.138519e-80 | 1.950595e-76 |
| MsG0780041691.01 | MsG0880043915.01 | 0.817914 | 2.575335e-52 | 1.900099e-49 |
| MsG0780041809.01 | MsG0880043915.01 | 0.813405 | 2.602471e-51 | 1.698059e-48 |
| MsG0880041908.01 | MsG0880043915.01 | 0.803309 | 3.706587e-49 | 1.856438e-46 |
| MsG0880043056.01 | MsG0880043915.01 | 0.810507 | 1.113632e-50 | 6.725148e-48 |
| MsG0880043915.01 | MsG0880044058.01 | -0.801934 | 7.123046e-49 | 3.444644e-46 |
| MsG0880043915.01 | MsG0880044317.01 | 0.802589 | 5.222218e-49 | 2.567951e-46 |
| MsG0880043915.01 | MsG0880044387.01 | 0.802751 | 4.834294e-49 | 2.387128e-46 |
| MsG0880043915.01 | MsG0880044548.01 | 0.801002 | 1.105715e-48 | 5.223085e-46 |
| MsG0880043915.01 | MsG0880045063.01 | 0.807537 | 4.815134e-50 | 2.688837e-47 |
| MsG0880043915.01 | MsG0880045703.01 | 0.851515 | 8.440353e-61 | 1.712596e-57 |
| MsG0880043915.01 | MsG0880046010.01 | 0.838685 | 2.483019e-57 | 3.350918e-54 |
| MsG0880043915.01 | MsG0880046341.01 | 0.820848 | 5.521236e-53 | 4.418868e-50 |
| MsG0280010606.01 | MsG0880043915.01 | 0.826355 | 2.839302e-54 | 2.656531e-51 |
| MsG0280011269.01 | MsG0880043915.01 | -0.800414 | 1.457313e-48 | 6.781757e-46 |
| MsG0280011281.01 | MsG0880043915.01 | 0.834473 | 2.936578e-56 | 3.488761e-53 |
| MsG0280011389.01 | MsG0880043915.01 | 0.803435 | 3.490483e-49 | 1.753908e-46 |
| MsG0380012148.01 | MsG0880043915.01 | 0.815644 | 8.316118e-52 | 5.766397e-49 |
| MsG0380013506.01 | MsG0880043915.01 | 0.800736 | 1.252886e-48 | 5.878059e-46 |
| MsG0380014231.01 | MsG0880043915.01 | 0.858552 | 7.619634e-63 | 1.952028e-59 |
| MsG0380014639.01 | MsG0880043915.01 | 0.803037 | 4.220017e-49 | 2.098895e-46 |
| MsG0380014642.01 | MsG0880043915.01 | 0.827316 | 1.673799e-54 | 1.610273e-51 |
| MsG0680031204.01 | MsG0880043915.01 | 0.823791 | 1.145282e-53 | 9.960972e-51 |
| MsG0680031449.01 | MsG0880043915.01 | 0.872307 | 3.515544e-67 | 1.463819e-63 |
| MsG0680031713.01 | MsG0880043915.01 | 0.806937 | 6.454866e-50 | 3.548672e-47 |
| MsG0680035584.01 | MsG0880043915.01 | 0.828759 | 7.518878e-55 | 7.542916e-52 |
| MsG0480020841.01 | MsG0880043915.01 | 0.812710 | 3.697716e-51 | 2.367924e-48 |
| MsG0480020842.01 | MsG0880043915.01 | 0.818105 | 2.331134e-52 | 1.728945e-49 |
| MsG0480020938.01 | MsG0880043915.01 | 0.819026 | 1.441369e-52 | 1.096680e-49 |
| MsG0480021497.01 | MsG0880043915.01 | 0.811480 | 6.855220e-51 | 4.248234e-48 |
| MsG0480021967.01 | MsG0880043915.01 | 0.864930 | 8.531795e-65 | 2.725519e-61 |
| MsG0480022411.01 | MsG0880043915.01 | 0.803157 | 3.985747e-49 | 1.988563e-46 |
| MsG0480022584.01 | MsG0880043915.01 | 0.801735 | 7.825776e-49 | 3.765306e-46 |
| MsG0480022687.01 | MsG0880043915.01 | 0.807020 | 6.198734e-50 | 3.415258e-47 |
| MsG0480023103.01 | MsG0880043915.01 | 0.841364 | 4.970235e-58 | 7.286248e-55 |
| MsG0480023104.01 | MsG0880043915.01 | 0.812399 | 4.323832e-51 | 2.746445e-48 |
| MsG0480023173.01 | MsG0880043915.01 | 0.866734 | 2.295881e-65 | 7.820087e-62 |
| MsG0480023466.01 | MsG0880043915.01 | 0.807949 | 3.936918e-50 | 2.222184e-47 |
| MsG0480023531.01 | MsG0880043915.01 | 0.836028 | 1.189817e-56 | 1.480900e-53 |
| MsG0480023704.01 | MsG0880043915.01 | 0.804466 | 2.131805e-49 | 1.099907e-46 |
| MsG0480023705.01 | MsG0880043915.01 | 0.801544 | 8.566306e-49 | 4.101824e-46 |
| MsG0480023851.01 | MsG0880043915.01 | 0.821971 | 3.039524e-53 | 2.510338e-50 |
| MsG0580024135.01 | MsG0880043915.01 | 0.814701 | 1.347444e-51 | 9.105628e-49 |
| MsG0580024442.01 | MsG0880043915.01 | 0.822539 | 2.243968e-53 | 1.883738e-50 |
| MsG0580024493.01 | MsG0880043915.01 | 0.800547 | 1.369117e-48 | 6.392569e-46 |
| MsG0580024678.01 | MsG0880043915.01 | 0.810544 | 1.093647e-50 | 6.610660e-48 |
| MsG0580025105.01 | MsG0880043915.01 | 0.838443 | 2.866896e-57 | 3.840687e-54 |
| MsG0580025153.01 | MsG0880043915.01 | 0.820206 | 7.751765e-53 | 6.094363e-50 |
| MsG0580025480.01 | MsG0880043915.01 | 0.818634 | 1.769088e-52 | 1.331375e-49 |
| MsG0580025486.01 | MsG0880043915.01 | 0.833995 | 3.871208e-56 | 4.533466e-53 |
| MsG0580025633.01 | MsG0880043915.01 | -0.811917 | 5.506570e-51 | 3.452000e-48 |
| MsG0380015456.01 | MsG0880043915.01 | 0.812876 | 3.399338e-51 | 2.186381e-48 |
| MsG0380016166.01 | MsG0880043915.01 | 0.847941 | 8.409127e-60 | 1.518758e-56 |
| MsG0380016241.01 | MsG0880043915.01 | 0.814715 | 1.337798e-51 | 9.043900e-49 |
| MsG0380016456.01 | MsG0880043915.01 | 0.852492 | 4.454675e-61 | 9.324354e-58 |
| MsG0380016568.01 | MsG0880043915.01 | 0.820433 | 6.877440e-53 | 5.441203e-50 |
| MsG0380016861.01 | MsG0880043915.01 | 0.810714 | 1.004909e-50 | 6.102364e-48 |
| MsG0380016934.01 | MsG0880043915.01 | 0.819255 | 1.278223e-52 | 9.786506e-50 |
| MsG0380017030.01 | MsG0880043915.01 | -0.808733 | 2.678303e-50 | 1.543152e-47 |
| MsG0380017773.01 | MsG0880043915.01 | 0.803041 | 4.212491e-49 | 2.095344e-46 |
| MsG0380017812.01 | MsG0880043915.01 | 0.808338 | 3.252401e-50 | 1.854842e-47 |
| MsG0380017962.01 | MsG0880043915.01 | 0.806608 | 7.574278e-50 | 4.128852e-47 |
| MsG0480018112.01 | MsG0880043915.01 | 0.825967 | 3.511416e-54 | 3.249326e-51 |
| MsG0480018202.01 | MsG0880043915.01 | 0.803773 | 2.970763e-49 | 1.505773e-46 |
| MsG0480018203.01 | MsG0880043915.01 | 0.805183 | 1.509300e-49 | 7.932917e-47 |
| MsG0580027276.01 | MsG0880043915.01 | 0.864373 | 1.274255e-64 | 3.991712e-61 |
| MsG0580028159.01 | MsG0880043915.01 | 0.810816 | 9.551213e-51 | 5.815722e-48 |
| MsG0580029172.01 | MsG0880043915.01 | 0.817277 | 3.585085e-52 | 2.599198e-49 |
| MsG0580029177.01 | MsG0880043915.01 | 0.836627 | 8.377038e-57 | 1.061606e-53 |
| MsG0580029751.01 | MsG0880043915.01 | 0.878662 | 2.342839e-69 | 1.233715e-65 |
| MsG0580030014.01 | MsG0880043915.01 | 0.818224 | 2.191718e-52 | 1.630841e-49 |
| MsG0680030635.01 | MsG0880043915.01 | 0.805841 | 1.098711e-49 | 5.873393e-47 |
| MsG0680030658.01 | MsG0880043915.01 | 0.820244 | 7.598772e-53 | 5.980126e-50 |
| MsG0280007288.01 | MsG0880043915.01 | 0.840219 | 9.919985e-58 | 1.403521e-54 |
| MsG0280007291.01 | MsG0880043915.01 | 0.831760 | 1.390148e-55 | 1.523255e-52 |
| MsG0280009079.01 | MsG0880043915.01 | 0.803538 | 3.324197e-49 | 1.674855e-46 |
| MsG0280009287.01 | MsG0880043915.01 | 0.802911 | 4.480230e-49 | 2.221299e-46 |
| MsG0280010149.01 | MsG0880043915.01 | 0.820276 | 7.470058e-53 | 5.884238e-50 |
| MsG0680035870.01 | MsG0880043915.01 | 0.809332 | 1.993824e-50 | 1.167334e-47 |
| MsG0780035994.01 | MsG0880043915.01 | 0.819658 | 1.034432e-52 | 8.009417e-50 |
| MsG0780039287.01 | MsG0880043915.01 | 0.800376 | 1.483666e-48 | 6.897756e-46 |
| MsG0780040072.01 | MsG0880043915.01 | 0.808953 | 2.403854e-50 | 1.393135e-47 |
| MsG0780040154.01 | MsG0880043915.01 | 0.823343 | 1.458119e-53 | 1.252026e-50 |
| MsG0780040167.01 | MsG0880043915.01 | 0.821831 | 3.275622e-53 | 2.694688e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043915.01.T01 | MTR_7g114660 | 85.648 | 216 | 23 | 2 | 111 | 318 | 175 | 390 | 4.20e-120 | 351 |
| MsG0880043915.01.T01 | MTR_7g114660 | 73.913 | 115 | 19 | 5 | 1 | 107 | 1 | 112 | 4.30e-41 | 147 |
| MsG0880043915.01.T01 | MTR_8g465010 | 82.857 | 210 | 29 | 1 | 116 | 318 | 176 | 385 | 7.39e-112 | 330 |
| MsG0880043915.01.T01 | MTR_8g465010 | 75.439 | 114 | 15 | 4 | 1 | 106 | 1 | 109 | 9.36e-41 | 146 |
| MsG0880043915.01.T01 | MTR_3g063950 | 51.675 | 209 | 64 | 4 | 144 | 315 | 111 | 319 | 1.66e-53 | 178 |
| MsG0880043915.01.T01 | MTR_3g063940 | 45.833 | 240 | 85 | 6 | 110 | 312 | 90 | 321 | 8.99e-53 | 176 |
| MsG0880043915.01.T01 | MTR_8g031500 | 52.941 | 204 | 32 | 5 | 124 | 318 | 197 | 345 | 1.36e-44 | 155 |
| MsG0880043915.01.T01 | MTR_8g031500 | 72.340 | 94 | 10 | 3 | 1 | 87 | 1 | 85 | 3.03e-34 | 128 |
| MsG0880043915.01.T01 | MTR_3g063940 | 40.741 | 189 | 88 | 6 | 110 | 282 | 90 | 270 | 7.08e-29 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043915.01.T01 | AT1G54520 | 67.143 | 210 | 61 | 2 | 117 | 318 | 182 | 391 | 6.24e-90 | 274 |
Find 78 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGTTTCTGAAGTTCTTTC+TGG | 0.173181 | 8:-32494504 | MsG0880043915.01.T01:CDS |
| TTCTTATAGGTTGGTTTGTT+GGG | 0.219017 | 8:-32494311 | MsG0880043915.01.T01:intron |
| CGGTTGCGAGAGATTCTAAT+AGG | 0.271867 | 8:+32494670 | None:intergenic |
| AGAGATTCTAATAGGGGTTT+TGG | 0.299744 | 8:+32494678 | None:intergenic |
| TGCAGGCAGTTGAGGTGTTA+TGG | 0.316865 | 8:-32493180 | MsG0880043915.01.T01:intron |
| AATCTCTCGCAACCGCAATA+TGG | 0.344151 | 8:-32494662 | MsG0880043915.01.T01:CDS |
| ATAGGTTGGTTTGTTGGGGA+TGG | 0.353730 | 8:-32494306 | MsG0880043915.01.T01:intron |
| TAATAATTTGCTCGAAAGAA+TGG | 0.355855 | 8:+32493281 | None:intergenic |
| GTTCTTATAGGTTGGTTTGT+TGG | 0.361203 | 8:-32494312 | MsG0880043915.01.T01:intron |
| ATTAGCTTTGCTTCGGCATC+CGG | 0.368451 | 8:-32494048 | MsG0880043915.01.T01:CDS |
| TATATATCAGGTAACAATCT+TGG | 0.380245 | 8:-32493382 | MsG0880043915.01.T01:intron |
| CCTGTCCCTTCCATTTGAAA+TGG | 0.386191 | 8:-32494839 | MsG0880043915.01.T01:CDS |
| ACGAGAAGCCAGAGATCTAA+TGG | 0.390065 | 8:-32493649 | MsG0880043915.01.T01:CDS |
| CACTTCAAAGGGATCTCAAT+AGG | 0.406748 | 8:-32494277 | MsG0880043915.01.T01:CDS |
| TGCTGAACCCATTAGATCTC+TGG | 0.409729 | 8:+32493641 | None:intergenic |
| TAGGTTGGTTTGTTGGGGAT+GGG | 0.411992 | 8:-32494305 | MsG0880043915.01.T01:CDS |
| AACCACCGTACTCAAGCTTC+AGG | 0.415007 | 8:-32494445 | MsG0880043915.01.T01:intron |
| AAATGATACTCTCTCGGAAC+TGG | 0.415575 | 8:-32493143 | MsG0880043915.01.T01:CDS |
| GGTAGTGTTCTTACTGCTGC+TGG | 0.422092 | 8:-32494471 | MsG0880043915.01.T01:CDS |
| CCATTTCAAATGGAAGGGAC+AGG | 0.424465 | 8:+32494839 | None:intergenic |
| GGTTGCGAGAGATTCTAATA+GGG | 0.428237 | 8:+32494671 | None:intergenic |
| ATTGCTTCTTCCATTTCAAA+TGG | 0.434252 | 8:+32494829 | None:intergenic |
| CTCGCTCTTCAATTGAAAGT+TGG | 0.436258 | 8:+32493725 | None:intergenic |
| CTTCTTCCATTTCAAATGGA+AGG | 0.439098 | 8:+32494833 | None:intergenic |
| CATCCGGATTACTGTATCTC+GGG | 0.450640 | 8:-32494032 | MsG0880043915.01.T01:CDS |
| TTTCGAATTGGAAGTTATTG+AGG | 0.459782 | 8:+32494701 | None:intergenic |
| CATAAACTTCCTAGCATCAA+TGG | 0.460567 | 8:-32493342 | MsG0880043915.01.T01:CDS |
| ATAAAGCGGGGTAGAGAAGA+AGG | 0.460921 | 8:-32493763 | MsG0880043915.01.T01:CDS |
| AGACCTCATTAGCTTTGCTT+CGG | 0.467947 | 8:-32494055 | MsG0880043915.01.T01:CDS |
| ACTGCTAAGGACGTCCTGTA+TGG | 0.483650 | 8:+32494576 | None:intergenic |
| GTAGCCGTGATATTAATTAC+AGG | 0.485838 | 8:-32494618 | MsG0880043915.01.T01:CDS |
| ATTTACAAAGGCAGCAAAAG+TGG | 0.489846 | 8:+32493103 | None:intergenic |
| ATTGGAAGTTATTGAGGAGG+GGG | 0.491268 | 8:+32494707 | None:intergenic |
| GGATGGGTCGCTCACTTCAA+AGG | 0.491818 | 8:-32494289 | MsG0880043915.01.T01:CDS |
| GATGGGTCGCTCACTTCAAA+GGG | 0.508481 | 8:-32494288 | MsG0880043915.01.T01:CDS |
| CAATGGAAGTGAAAACTTGA+AGG | 0.508838 | 8:-32493325 | MsG0880043915.01.T01:CDS |
| TAATTACTTGCAGGCAGTTG+AGG | 0.519740 | 8:-32493188 | MsG0880043915.01.T01:intron |
| CGAGAAGCCAGAGATCTAAT+GGG | 0.520093 | 8:-32493648 | MsG0880043915.01.T01:CDS |
| TTCTTCCATTTCAAATGGAA+GGG | 0.520484 | 8:+32494834 | None:intergenic |
| CATTTCAAATGGAAGGGACA+GGG | 0.526140 | 8:+32494840 | None:intergenic |
| GCATCCGGATTACTGTATCT+CGG | 0.526629 | 8:-32494033 | MsG0880043915.01.T01:CDS |
| GTTCTTTCTGGTTGTGATGA+GGG | 0.526982 | 8:-32494492 | MsG0880043915.01.T01:CDS |
| TTTGCAGGTGGACATAAAGC+GGG | 0.528200 | 8:-32493776 | MsG0880043915.01.T01:intron |
| TATTGATGTACGATCCATAC+AGG | 0.540984 | 8:-32494590 | MsG0880043915.01.T01:CDS |
| ACAATCTTGGCCGCTGCTGA+AGG | 0.542452 | 8:-32493369 | MsG0880043915.01.T01:CDS |
| AGTTCTTTCTGGTTGTGATG+AGG | 0.542880 | 8:-32494493 | MsG0880043915.01.T01:CDS |
| TCTTATAGGTTGGTTTGTTG+GGG | 0.543009 | 8:-32494310 | MsG0880043915.01.T01:intron |
| TCGAAGTGTGTTCCATATTG+CGG | 0.543575 | 8:+32494650 | None:intergenic |
| AAATGAAAATGATACTCTCT+CGG | 0.552119 | 8:-32493149 | MsG0880043915.01.T01:CDS |
| GAATTGGAAGTTATTGAGGA+GGG | 0.552364 | 8:+32494705 | None:intergenic |
| TCAACGTTAACAACATAAAG+AGG | 0.553658 | 8:-32493678 | MsG0880043915.01.T01:CDS |
| TACCCCGAGATACAGTAATC+CGG | 0.558063 | 8:+32494029 | None:intergenic |
| TAAAGCGGGGTAGAGAAGAA+GGG | 0.560416 | 8:-32493762 | MsG0880043915.01.T01:CDS |
| ATGCCGAAGCAAAGCTAATG+AGG | 0.567725 | 8:+32494052 | None:intergenic |
| CGAATTGGAAGTTATTGAGG+AGG | 0.572102 | 8:+32494704 | None:intergenic |
| AATTGGAAGTTATTGAGGAG+GGG | 0.572596 | 8:+32494706 | None:intergenic |
| GTTGCGAGAGATTCTAATAG+GGG | 0.574346 | 8:+32494672 | None:intergenic |
| AATTCCTGTAATTAATATCA+CGG | 0.575253 | 8:+32494614 | None:intergenic |
| AATTACCTGAAGCTTGAGTA+CGG | 0.580346 | 8:+32494440 | None:intergenic |
| ATTAATATCACGGCTACCGA+GGG | 0.582968 | 8:+32494624 | None:intergenic |
| AAAGCGGGGTAGAGAAGAAG+GGG | 0.592515 | 8:-32493761 | MsG0880043915.01.T01:CDS |
| TTTACAAAGGCAGCAAAAGT+GGG | 0.593284 | 8:+32493104 | None:intergenic |
| CAAACTTGTGGACATAGTCA+TGG | 0.601857 | 8:+32494872 | None:intergenic |
| AATGCCATTGCAATTTACAA+AGG | 0.605054 | 8:+32493091 | None:intergenic |
| TATATTGTAAGAAACTGCTA+AGG | 0.608677 | 8:+32494563 | None:intergenic |
| GGGTTCTAGTGACAAACTTG+TGG | 0.610609 | 8:+32494860 | None:intergenic |
| AAAATTTGATGAAGAGACGC+TGG | 0.618378 | 8:-32493701 | MsG0880043915.01.T01:CDS |
| CAACTTTCAATTGAAGAGCG+AGG | 0.618395 | 8:-32493724 | MsG0880043915.01.T01:CDS |
| GTTCAGCAATGAATACATAG+TGG | 0.625878 | 8:-32493626 | MsG0880043915.01.T01:intron |
| GCAGATACATCATCTCCAGA+AGG | 0.629689 | 8:-32494242 | MsG0880043915.01.T01:intron |
| AATTAATATCACGGCTACCG+AGG | 0.630032 | 8:+32494623 | None:intergenic |
| GAACACACTTCGAAAACCCT+CGG | 0.634501 | 8:-32494640 | MsG0880043915.01.T01:CDS |
| TATTGATATTGAAGTTGTCA+AGG | 0.636338 | 8:+32494785 | None:intergenic |
| GTTGTCAAGGTGTGAGTGCG+AGG | 0.654476 | 8:+32494798 | None:intergenic |
| ATCCGGATTACTGTATCTCG+GGG | 0.671601 | 8:-32494031 | MsG0880043915.01.T01:CDS |
| TTTATGTTCTCCTTCAGCAG+CGG | 0.687981 | 8:+32493359 | None:intergenic |
| TTGCAGGTGGACATAAAGCG+GGG | 0.690419 | 8:-32493775 | MsG0880043915.01.T01:intron |
| TACCTGAAGCTTGAGTACGG+TGG | 0.739281 | 8:+32494443 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGACTTAAAAAAATTAACTT+TGG | - | Chr8:32494427-32494446 | MsG0880043915.01.T01:intron | 15.0% |
| !! | GACTTAAAAAAATTAACTTT+GGG | - | Chr8:32494428-32494447 | MsG0880043915.01.T01:intron | 15.0% |
| !!! | ACAATATATTTTTCATCTTT+GGG | - | Chr8:32493427-32493446 | MsG0880043915.01.T01:intron | 15.0% |
| !!! | TACAATATATTTTTCATCTT+TGG | - | Chr8:32493426-32493445 | MsG0880043915.01.T01:intron | 15.0% |
| !! | AATTCCTGTAATTAATATCA+CGG | + | Chr8:32493364-32493383 | None:intergenic | 20.0% |
| !! | ATAGTATATGCTTAAATCTT+TGG | - | Chr8:32493612-32493631 | MsG0880043915.01.T01:intron | 20.0% |
| !! | ATTTATTAGAGTTCATCATT+AGG | - | Chr8:32494083-32494102 | MsG0880043915.01.T01:intron | 20.0% |
| !! | GAAATTGAGTTTATAGAAAT+TGG | - | Chr8:32494147-32494166 | MsG0880043915.01.T01:intron | 20.0% |
| !! | TTATACTTACTCTATATTGT+TGG | - | Chr8:32494111-32494130 | MsG0880043915.01.T01:intron | 20.0% |
| !! | TTTATTAGAGTTCATCATTA+GGG | - | Chr8:32494084-32494103 | MsG0880043915.01.T01:intron | 20.0% |
| !!! | ATTTTTCTATAATTACTTGC+AGG | - | Chr8:32494778-32494797 | MsG0880043915.01.T01:CDS | 20.0% |
| !!! | CAATATATTTTTCATCTTTG+GGG | - | Chr8:32493428-32493447 | MsG0880043915.01.T01:intron | 20.0% |
| !!! | TTGTTTGATTTTATGTTGTT+GGG | + | Chr8:32493216-32493235 | None:intergenic | 20.0% |
| !!! | TTTGTTTGATTTTATGTTGT+TGG | + | Chr8:32493217-32493236 | None:intergenic | 20.0% |
| ! | AAATGAAAATGATACTCTCT+CGG | - | Chr8:32494826-32494845 | MsG0880043915.01.T01:CDS | 25.0% |
| ! | AACAACAAAGTTTACAAGAT+AGG | + | Chr8:32494571-32494590 | None:intergenic | 25.0% |
| ! | AACCACATGATAATACTTTA+AGG | - | Chr8:32493841-32493860 | MsG0880043915.01.T01:intron | 25.0% |
| ! | AATATGTAATTCAAACCTTC+TGG | + | Chr8:32493751-32493770 | None:intergenic | 25.0% |
| ! | TATATATCAGGTAACAATCT+TGG | - | Chr8:32494593-32494612 | MsG0880043915.01.T01:CDS | 25.0% |
| ! | TATATTGTAAGAAACTGCTA+AGG | + | Chr8:32493415-32493434 | None:intergenic | 25.0% |
| ! | TATTGATATTGAAGTTGTCA+AGG | + | Chr8:32493193-32493212 | None:intergenic | 25.0% |
| ! | TATTGTTGGCATAGAAATAA+AGG | - | Chr8:32494125-32494144 | MsG0880043915.01.T01:intron | 25.0% |
| ! | TTTAAGTCTATAGTGCATAA+AGG | + | Chr8:32494416-32494435 | None:intergenic | 25.0% |
| !! | AACCTTAAAGTATTATCATG+TGG | + | Chr8:32493846-32493865 | None:intergenic | 25.0% |
| !! | AATGTTAGTGTTGTTCTTAT+AGG | - | Chr8:32493651-32493670 | MsG0880043915.01.T01:CDS | 25.0% |
| !! | AGTATCATTTTCATTTTGTG+GGG | + | Chr8:32494822-32494841 | None:intergenic | 25.0% |
| !! | ATATTTTCTGACATTTTTGC+AGG | - | Chr8:32494184-32494203 | MsG0880043915.01.T01:intron | 25.0% |
| !! | GAGTATCATTTTCATTTTGT+GGG | + | Chr8:32494823-32494842 | None:intergenic | 25.0% |
| !! | TAAAGTATTATCATGTGGTT+AGG | + | Chr8:32493841-32493860 | None:intergenic | 25.0% |
| !!! | AGAGTATCATTTTCATTTTG+TGG | + | Chr8:32494824-32494843 | None:intergenic | 25.0% |
| !!! | CACTTTTTCAAGTGATTATA+GGG | + | Chr8:32494527-32494546 | None:intergenic | 25.0% |
| !!! | CTTTGTTGTTCTTATATATC+AGG | - | Chr8:32494581-32494600 | MsG0880043915.01.T01:CDS | 25.0% |
| !!! | TAATAATTTGCTCGAAAGAA+TGG | + | Chr8:32494697-32494716 | None:intergenic | 25.0% |
| !!! | TCACTTTTTCAAGTGATTAT+AGG | + | Chr8:32494528-32494547 | None:intergenic | 25.0% |
| AACAACATCTGAAAATGACT+TGG | + | Chr8:32494738-32494757 | None:intergenic | 30.0% | |
| AAGGAAGCTTTACAAAAACT+TGG | - | Chr8:32494669-32494688 | MsG0880043915.01.T01:CDS | 30.0% | |
| AGGAATACATGAAATTGCAA+TGG | + | Chr8:32494006-32494025 | None:intergenic | 30.0% | |
| ATTGCAATTTCATGTATTCC+TGG | - | Chr8:32494005-32494024 | MsG0880043915.01.T01:intron | 30.0% | |
| ATTGCTTCTTCCATTTCAAA+TGG | + | Chr8:32493149-32493168 | None:intergenic | 30.0% | |
| GGTTTGAATTACATATTGAC+AGG | - | Chr8:32493754-32493773 | MsG0880043915.01.T01:CDS | 30.0% | |
| TAGTGGTATCATAACTATCT+TGG | - | Chr8:32494366-32494385 | MsG0880043915.01.T01:intron | 30.0% | |
| TCAACGTTAACAACATAAAG+AGG | - | Chr8:32494297-32494316 | MsG0880043915.01.T01:CDS | 30.0% | |
| TGACCAAAAATCACATGTTT+AGG | + | Chr8:32493794-32493813 | None:intergenic | 30.0% | |
| TTCTTCCATTTCAAATGGAA+GGG | + | Chr8:32493144-32493163 | None:intergenic | 30.0% | |
| TTTCGAATTGGAAGTTATTG+AGG | + | Chr8:32493277-32493296 | None:intergenic | 30.0% | |
| ! | TTAGTGTTGTTCTTATAGGT+TGG | - | Chr8:32493655-32493674 | MsG0880043915.01.T01:CDS | 30.0% |
| !! | TATATTTTTCATCTTTGGGG+CGG | - | Chr8:32493431-32493450 | MsG0880043915.01.T01:intron | 30.0% |
| !! | TTCTTATAGGTTGGTTTGTT+GGG | - | Chr8:32493664-32493683 | MsG0880043915.01.T01:CDS | 30.0% |
| AAAATTTGATGAAGAGACGC+TGG | - | Chr8:32494274-32494293 | MsG0880043915.01.T01:CDS | 35.0% | |
| AATTACCTGAAGCTTGAGTA+CGG | + | Chr8:32493538-32493557 | None:intergenic | 35.0% | |
| AATTGGAAGTTATTGAGGAG+GGG | + | Chr8:32493272-32493291 | None:intergenic | 35.0% | |
| CAATGGAAGTGAAAACTTGA+AGG | - | Chr8:32494650-32494669 | MsG0880043915.01.T01:CDS | 35.0% | |
| CATAAACTTCCTAGCATCAA+TGG | - | Chr8:32494633-32494652 | MsG0880043915.01.T01:CDS | 35.0% | |
| CCAAAAATCACATGTTTAGG+AGG | + | Chr8:32493791-32493810 | None:intergenic | 35.0% | |
| CTTCTTCCATTTCAAATGGA+AGG | + | Chr8:32493145-32493164 | None:intergenic | 35.0% | |
| GAATTGGAAGTTATTGAGGA+GGG | + | Chr8:32493273-32493292 | None:intergenic | 35.0% | |
| GTAGCCGTGATATTAATTAC+AGG | - | Chr8:32493357-32493376 | MsG0880043915.01.T01:CDS | 35.0% | |
| GTTCAGCAATGAATACATAG+TGG | - | Chr8:32494349-32494368 | MsG0880043915.01.T01:intron | 35.0% | |
| TATTGATGTACGATCCATAC+AGG | - | Chr8:32493385-32493404 | MsG0880043915.01.T01:intron | 35.0% | |
| TGAAAAAGTGAAGTTCCATG+AGG | - | Chr8:32494537-32494556 | MsG0880043915.01.T01:CDS | 35.0% | |
| ! | AGAGATTCTAATAGGGGTTT+TGG | + | Chr8:32493300-32493319 | None:intergenic | 35.0% |
| ! | CCTCCTAAACATGTGATTTT+TGG | - | Chr8:32493788-32493807 | MsG0880043915.01.T01:intron | 35.0% |
| ! | TTTTCACTTCCATTGATGCT+AGG | + | Chr8:32494645-32494664 | None:intergenic | 35.0% |
| ! | TTTTCTGACATTTTTGCAGG+TGG | - | Chr8:32494187-32494206 | MsG0880043915.01.T01:intron | 35.0% |
| !! | GTTCTTATAGGTTGGTTTGT+TGG | - | Chr8:32493663-32493682 | MsG0880043915.01.T01:CDS | 35.0% |
| !! | TCTTATAGGTTGGTTTGTTG+GGG | - | Chr8:32493665-32493684 | MsG0880043915.01.T01:CDS | 35.0% |
| !! | TTCGTTTCTGAAGTTCTTTC+TGG | - | Chr8:32493471-32493490 | MsG0880043915.01.T01:intron | 35.0% |
| !!! | ATATTTTTCATCTTTGGGGC+GGG | - | Chr8:32493432-32493451 | MsG0880043915.01.T01:intron | 35.0% |
| AAAGTGAAGTTCCATGAGGA+TGG | - | Chr8:32494541-32494560 | MsG0880043915.01.T01:CDS | 40.0% | |
| AAATGATACTCTCTCGGAAC+TGG | - | Chr8:32494832-32494851 | MsG0880043915.01.T01:CDS | 40.0% | |
| AATTAATATCACGGCTACCG+AGG | + | Chr8:32493355-32493374 | None:intergenic | 40.0% | |
| ATTAATATCACGGCTACCGA+GGG | + | Chr8:32493354-32493373 | None:intergenic | 40.0% | |
| ATTGGAAGTTATTGAGGAGG+GGG | + | Chr8:32493271-32493290 | None:intergenic | 40.0% | |
| CAACTTTCAATTGAAGAGCG+AGG | - | Chr8:32494251-32494270 | MsG0880043915.01.T01:CDS | 40.0% | |
| CACTTCAAAGGGATCTCAAT+AGG | - | Chr8:32493698-32493717 | MsG0880043915.01.T01:CDS | 40.0% | |
| CATTTCAAATGGAAGGGACA+GGG | + | Chr8:32493138-32493157 | None:intergenic | 40.0% | |
| CGAATTGGAAGTTATTGAGG+AGG | + | Chr8:32493274-32493293 | None:intergenic | 40.0% | |
| CTCGCTCTTCAATTGAAAGT+TGG | + | Chr8:32494253-32494272 | None:intergenic | 40.0% | |
| GGTTGCGAGAGATTCTAATA+GGG | + | Chr8:32493307-32493326 | None:intergenic | 40.0% | |
| GTTGCGAGAGATTCTAATAG+GGG | + | Chr8:32493306-32493325 | None:intergenic | 40.0% | |
| TAATTACTTGCAGGCAGTTG+AGG | - | Chr8:32494787-32494806 | MsG0880043915.01.T01:CDS | 40.0% | |
| TCGAAGTGTGTTCCATATTG+CGG | + | Chr8:32493328-32493347 | None:intergenic | 40.0% | |
| TTTATGTTCTCCTTCAGCAG+CGG | + | Chr8:32494619-32494638 | None:intergenic | 40.0% | |
| ! | TTTTGCAGGTGGACATAAAG+CGG | - | Chr8:32494198-32494217 | MsG0880043915.01.T01:intron | 40.0% |
| !! | AGACCTCATTAGCTTTGCTT+CGG | - | Chr8:32493920-32493939 | MsG0880043915.01.T01:intron | 40.0% |
| !! | AGTTCTTTCTGGTTGTGATG+AGG | - | Chr8:32493482-32493501 | MsG0880043915.01.T01:intron | 40.0% |
| !! | GTTCTTTCTGGTTGTGATGA+GGG | - | Chr8:32493483-32493502 | MsG0880043915.01.T01:intron | 40.0% |
| !!! | TAGGGGTTTTGGTTTCGAAT+TGG | + | Chr8:32493289-32493308 | None:intergenic | 40.0% |
| AATCTCTCGCAACCGCAATA+TGG | - | Chr8:32493313-32493332 | MsG0880043915.01.T01:CDS | 45.0% | |
| AATGAGGTCTCTGCAACAGA+AGG | + | Chr8:32493910-32493929 | None:intergenic | 45.0% | |
| ACGAGAAGCCAGAGATCTAA+TGG | - | Chr8:32494326-32494345 | MsG0880043915.01.T01:intron | 45.0% | |
| AGATAGGTGAACCATCCTCA+TGG | + | Chr8:32494555-32494574 | None:intergenic | 45.0% | |
| ATAAAGCGGGGTAGAGAAGA+AGG | - | Chr8:32494212-32494231 | MsG0880043915.01.T01:intron | 45.0% | |
| ATCCGGATTACTGTATCTCG+GGG | - | Chr8:32493944-32493963 | MsG0880043915.01.T01:intron | 45.0% | |
| ATGCCGAAGCAAAGCTAATG+AGG | + | Chr8:32493926-32493945 | None:intergenic | 45.0% | |
| CATCCGGATTACTGTATCTC+GGG | - | Chr8:32493943-32493962 | MsG0880043915.01.T01:intron | 45.0% | |
| CCATTTCAAATGGAAGGGAC+AGG | + | Chr8:32493139-32493158 | None:intergenic | 45.0% | |
| CCTGTCCCTTCCATTTGAAA+TGG | - | Chr8:32493136-32493155 | MsG0880043915.01.T01:CDS | 45.0% | |
| CGAGAAGCCAGAGATCTAAT+GGG | - | Chr8:32494327-32494346 | MsG0880043915.01.T01:intron | 45.0% | |
| CGGTTGCGAGAGATTCTAAT+AGG | + | Chr8:32493308-32493327 | None:intergenic | 45.0% | |
| GAACACACTTCGAAAACCCT+CGG | - | Chr8:32493335-32493354 | MsG0880043915.01.T01:CDS | 45.0% | |
| GCAGATACATCATCTCCAGA+AGG | - | Chr8:32493733-32493752 | MsG0880043915.01.T01:CDS | 45.0% | |
| GCATCCGGATTACTGTATCT+CGG | - | Chr8:32493942-32493961 | MsG0880043915.01.T01:intron | 45.0% | |
| TAAAGCGGGGTAGAGAAGAA+GGG | - | Chr8:32494213-32494232 | MsG0880043915.01.T01:intron | 45.0% | |
| TACCCCGAGATACAGTAATC+CGG | + | Chr8:32493949-32493968 | None:intergenic | 45.0% | |
| TGCTGAACCCATTAGATCTC+TGG | + | Chr8:32494337-32494356 | None:intergenic | 45.0% | |
| TTTGCAGGTGGACATAAAGC+GGG | - | Chr8:32494199-32494218 | MsG0880043915.01.T01:intron | 45.0% | |
| ! | GGGTTCTAGTGACAAACTTG+TGG | + | Chr8:32493118-32493137 | None:intergenic | 45.0% |
| !! | AGGAGGGGGTTTTGTGATTT+TGG | + | Chr8:32493257-32493276 | None:intergenic | 45.0% |
| !! | ATAGGTTGGTTTGTTGGGGA+TGG | - | Chr8:32493669-32493688 | MsG0880043915.01.T01:CDS | 45.0% |
| !! | ATTAGCTTTGCTTCGGCATC+CGG | - | Chr8:32493927-32493946 | MsG0880043915.01.T01:intron | 45.0% |
| !! | TAGGTTGGTTTGTTGGGGAT+GGG | - | Chr8:32493670-32493689 | MsG0880043915.01.T01:CDS | 45.0% |
| AAAGCGGGGTAGAGAAGAAG+GGG | - | Chr8:32494214-32494233 | MsG0880043915.01.T01:intron | 50.0% | |
| AACCACCGTACTCAAGCTTC+AGG | - | Chr8:32493530-32493549 | MsG0880043915.01.T01:intron | 50.0% | |
| AAGACGTCGTCGTCTATTCC+AGG | + | Chr8:32494026-32494045 | None:intergenic | 50.0% | |
| ACTGCTAAGGACGTCCTGTA+TGG | + | Chr8:32493402-32493421 | None:intergenic | 50.0% | |
| GATGGGTCGCTCACTTCAAA+GGG | - | Chr8:32493687-32493706 | MsG0880043915.01.T01:CDS | 50.0% | |
| GGTAGTGTTCTTACTGCTGC+TGG | - | Chr8:32493504-32493523 | MsG0880043915.01.T01:intron | 50.0% | |
| TACCTGAAGCTTGAGTACGG+TGG | + | Chr8:32493535-32493554 | None:intergenic | 50.0% | |
| TTGCAGGTGGACATAAAGCG+GGG | - | Chr8:32494200-32494219 | MsG0880043915.01.T01:intron | 50.0% | |
| ! | TGCAGGCAGTTGAGGTGTTA+TGG | - | Chr8:32494795-32494814 | MsG0880043915.01.T01:CDS | 50.0% |
| ACAATCTTGGCCGCTGCTGA+AGG | - | Chr8:32494606-32494625 | MsG0880043915.01.T01:CDS | 55.0% | |
| GGATGGGTCGCTCACTTCAA+AGG | - | Chr8:32493686-32493705 | MsG0880043915.01.T01:CDS | 55.0% | |
| GTTGTCAAGGTGTGAGTGCG+AGG | + | Chr8:32493180-32493199 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 32493105 | 32494892 | 32493105 | ID=MsG0880043915.01;Name=MsG0880043915.01 |
| Chr8 | mRNA | 32493105 | 32494892 | 32493105 | ID=MsG0880043915.01.T01;Parent=MsG0880043915.01;Name=MsG0880043915.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|6|0|318 |
| Chr8 | exon | 32494446 | 32494892 | 32494446 | ID=MsG0880043915.01.T01:exon:7975;Parent=MsG0880043915.01.T01 |
| Chr8 | exon | 32494243 | 32494324 | 32494243 | ID=MsG0880043915.01.T01:exon:7974;Parent=MsG0880043915.01.T01 |
| Chr8 | exon | 32494022 | 32494077 | 32494022 | ID=MsG0880043915.01.T01:exon:7973;Parent=MsG0880043915.01.T01 |
| Chr8 | exon | 32493627 | 32493791 | 32493627 | ID=MsG0880043915.01.T01:exon:7972;Parent=MsG0880043915.01.T01 |
| Chr8 | exon | 32493281 | 32493394 | 32493281 | ID=MsG0880043915.01.T01:exon:7971;Parent=MsG0880043915.01.T01 |
| Chr8 | exon | 32493105 | 32493197 | 32493105 | ID=MsG0880043915.01.T01:exon:7970;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32494446 | 32494892 | 32494446 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32494243 | 32494324 | 32494243 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32494022 | 32494077 | 32494022 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32493627 | 32493791 | 32493627 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32493281 | 32493394 | 32493281 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Chr8 | CDS | 32493105 | 32493197 | 32493105 | ID=MsG0880043915.01.T01:cds;Parent=MsG0880043915.01.T01 |
| Gene Sequence |
| Protein sequence |
