AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044387.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044387.01.T01 MTR_8g061130 95.946 148 5 1 1 148 1 147 4.38e-100 283
MsG0880044387.01.T01 MTR_0002s0770 73.529 68 18 0 72 139 15 82 1.65e-31 109
MsG0880044387.01.T02 MTR_8g061130 98.333 60 1 0 1 60 88 147 1.86e-37 121
MsG0880044387.01.T02 MTR_0002s0770 76.471 51 12 0 1 51 32 82 6.93e-22 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044387.01.T01 AT4G11175 69.014 71 22 0 69 139 67 137 1.03e-30 108
MsG0880044387.01.T02 AT4G11175 70.588 51 15 0 1 51 87 137 6.31e-21 79.7

Find 67 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATGGTGGAGTGGGGTTT+AGG 0.216308 8:-41176032 None:intergenic
CCAAAGGACGCATCATTTAT+AGG 0.221209 8:+41176277 MsG0880044387.01.T01:CDS
GGATGGTGGAGTGGGGTTTA+GGG 0.277606 8:-41176031 None:intergenic
GATGGTGGGCGGAGGAAGGA+TGG 0.340324 8:-41176006 None:intergenic
AGGAGTGGGTGGTGAATGAT+TGG 0.367617 8:-41175948 None:intergenic
GTTGGCGATGGTGAATGATT+GGG 0.370868 8:-41175918 None:intergenic
TGGGGTGCGAAAGTGGAGAG+TGG 0.374140 8:-41175986 None:intergenic
GAATGATTGGGGCGTGGAAT+GGG 0.377126 8:-41175906 None:intergenic
CCTATAAATGATGCGTCCTT+TGG 0.381193 8:-41176277 None:intergenic
AATTACGTTCGGATCCTTCC+TGG 0.385097 8:+41176216 MsG0880044387.01.T01:CDS
TGGTGGAGTGGGGTTTAGGG+GGG 0.388729 8:-41176028 None:intergenic
GATGGTGGAGTGGGGTTTAG+GGG 0.391934 8:-41176030 None:intergenic
ATGGTGGGCGGAGGAAGGAT+GGG 0.397127 8:-41176005 None:intergenic
ACAAAGCCAGCGAGCAAAAG+TGG 0.399064 8:+41176088 MsG0880044387.01.T01:CDS
ATGGTGGAGTGGGGTTTAGG+GGG 0.407363 8:-41176029 None:intergenic
GGTTGGCGATGGTGAATGAT+TGG 0.410559 8:-41175919 None:intergenic
TGGTGGGCGGAGGAAGGATG+GGG 0.418471 8:-41176004 None:intergenic
CGAACACGGAACATGCCGTT+AGG 0.422494 8:-41176141 None:intergenic
GGATCCGAACGTAATTCTTT+CGG 0.427721 8:-41176209 None:intergenic
CTTATCCTTGGATATATCTC+TGG 0.434923 8:+41176180 MsG0880044387.01.T01:CDS
GTGGTGAATGATTGGGTAGA+GGG 0.438792 8:-41175940 None:intergenic
GTGAAGATGGATGGTGGAGT+GGG 0.440957 8:-41176039 None:intergenic
GGAAGGATGGGGTGCGAAAG+TGG 0.455893 8:-41175993 None:intergenic
TGAATGATTGGGGCGTGGAA+TGG 0.456507 8:-41175907 None:intergenic
ATAAATGATGCGTCCTTTGG+AGG 0.459795 8:-41176274 None:intergenic
GGGGTGCGAAAGTGGAGAGT+GGG 0.461179 8:-41175985 None:intergenic
CAGAGATATATCCAAGGATA+AGG 0.465359 8:-41176179 None:intergenic
GGGTGCGAAAGTGGAGAGTG+GGG 0.467038 8:-41175984 None:intergenic
CTTGGCGGAGACGGTGAAGA+TGG 0.483060 8:-41176052 None:intergenic
GATAATGAAGACCTTATCCT+TGG 0.484697 8:+41176168 MsG0880044387.01.T01:CDS
TATAGGCTTCGTAATACTCC+TGG 0.487758 8:+41176294 MsG0880044387.01.T01:CDS
GGTTGAGGACTTGGCGGAGA+CGG 0.491903 8:-41176061 None:intergenic
GATTGGGGCGTGGAATGGGA+TGG 0.497058 8:-41175902 None:intergenic
GGTGAAGATGGATGGTGGAG+TGG 0.509570 8:-41176040 None:intergenic
TTTGCTCGCTGGCTTTGTCG+GGG 0.527845 8:-41176083 None:intergenic
AACTAAGAAGAGCCACCACC+AGG 0.533810 8:-41176312 None:intergenic
TGAATGATTGGGTAGAGGGT+TGG 0.551981 8:-41175936 None:intergenic
CTTCGTAATACTCCTGGTGG+TGG 0.553297 8:+41176300 MsG0880044387.01.T01:CDS
TTGGCGATGGTGAATGATTG+GGG 0.557416 8:-41175917 None:intergenic
GGTGGTGAATGATTGGGTAG+AGG 0.559640 8:-41175941 None:intergenic
GTGGAATGGGATGGAAGATG+AGG 0.564521 8:-41175893 None:intergenic
TTGCTCGCTGGCTTTGTCGG+GGG 0.564722 8:-41176082 None:intergenic
TGAAGATGGATGGTGGAGTG+GGG 0.567905 8:-41176038 None:intergenic
GGATCCGAAAGAATTACGTT+CGG 0.574169 8:+41176205 MsG0880044387.01.T01:CDS
CACGGAACATGCCGTTAGGG+AGG 0.578464 8:-41176137 None:intergenic
GAACACGGAACATGCCGTTA+GGG 0.578793 8:-41176140 None:intergenic
AGTCGATATGATTCCTCCAA+AGG 0.579977 8:+41176261 MsG0880044387.01.T01:CDS
CTTCATTATCGAGACGAACA+CGG 0.580715 8:-41176155 None:intergenic
TCAACTCTAACTCTATCTCC+AGG 0.592855 8:-41176234 None:intergenic
ATCACAGAATCCCTCCCTAA+CGG 0.596048 8:+41176126 MsG0880044387.01.T01:CDS
GCGGAGACGGTGAAGATGGA+TGG 0.600621 8:-41176048 None:intergenic
GGAGTGGGTGGTGAATGATT+GGG 0.606231 8:-41175947 None:intergenic
ATTGGGTAGAGGGTTGGCGA+TGG 0.606708 8:-41175930 None:intergenic
CAAAGCCAGCGAGCAAAAGT+GGG 0.609253 8:+41176089 MsG0880044387.01.T01:CDS
GGTGCGAAAGTGGAGAGTGG+GGG 0.609773 8:-41175983 None:intergenic
CTCTAACTCTATCTCCAGGA+AGG 0.615980 8:-41176230 None:intergenic
GGGTGTGGAAAGAAAAGGAG+TGG 0.621177 8:-41175963 None:intergenic
GTGGAAAGAAAAGGAGTGGG+TGG 0.622441 8:-41175959 None:intergenic
GAGACGGTGAAGATGGATGG+TGG 0.626813 8:-41176045 None:intergenic
ATTCTGTGATGAGACCTTCG+TGG 0.627359 8:-41176113 None:intergenic
ACGGAACATGCCGTTAGGGA+GGG 0.631389 8:-41176136 None:intergenic
GATGGTGAATGATTGGGGCG+TGG 0.638062 8:-41175912 None:intergenic
TCCTTGGATATATCTCTGGA+AGG 0.641053 8:+41176184 MsG0880044387.01.T01:CDS
TCCTTCCAGAGATATATCCA+AGG 0.654970 8:-41176185 None:intergenic
GGTGTGGAAAGAAAAGGAGT+GGG 0.666040 8:-41175962 None:intergenic
GAGCAAAAGTGGGTCCACGA+AGG 0.697991 8:+41176099 MsG0880044387.01.T01:CDS
AGGCTTCGTAATACTCCTGG+TGG 0.702905 8:+41176297 MsG0880044387.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAGAGATATATCCAAGGATA+AGG - Chr8:41176182-41176201 None:intergenic 35.0%
CTTATCCTTGGATATATCTC+TGG + Chr8:41176180-41176199 MsG0880044387.01.T01:CDS 35.0%
GATAATGAAGACCTTATCCT+TGG + Chr8:41176168-41176187 MsG0880044387.01.T01:CDS 35.0%
AGTCGATATGATTCCTCCAA+AGG + Chr8:41176261-41176280 MsG0880044387.01.T01:CDS 40.0%
ATAAATGATGCGTCCTTTGG+AGG - Chr8:41176277-41176296 None:intergenic 40.0%
CCAAAGGACGCATCATTTAT+AGG + Chr8:41176277-41176296 MsG0880044387.01.T01:CDS 40.0%
CCTATAAATGATGCGTCCTT+TGG - Chr8:41176280-41176299 None:intergenic 40.0%
CTTCATTATCGAGACGAACA+CGG - Chr8:41176158-41176177 None:intergenic 40.0%
GGATCCGAAAGAATTACGTT+CGG + Chr8:41176205-41176224 MsG0880044387.01.T01:CDS 40.0%
GGATCCGAACGTAATTCTTT+CGG - Chr8:41176212-41176231 None:intergenic 40.0%
TATAGGCTTCGTAATACTCC+TGG + Chr8:41176294-41176313 MsG0880044387.01.T01:CDS 40.0%
TCAACTCTAACTCTATCTCC+AGG - Chr8:41176237-41176256 None:intergenic 40.0%
TCCTTCCAGAGATATATCCA+AGG - Chr8:41176188-41176207 None:intergenic 40.0%
TCCTTGGATATATCTCTGGA+AGG + Chr8:41176184-41176203 MsG0880044387.01.T01:CDS 40.0%
AATTACGTTCGGATCCTTCC+TGG + Chr8:41176216-41176235 MsG0880044387.01.T01:CDS 45.0%
ATCACAGAATCCCTCCCTAA+CGG + Chr8:41176126-41176145 MsG0880044387.01.T01:CDS 45.0%
ATTCTGTGATGAGACCTTCG+TGG - Chr8:41176116-41176135 None:intergenic 45.0%
CTCTAACTCTATCTCCAGGA+AGG - Chr8:41176233-41176252 None:intergenic 45.0%
GGTGTGGAAAGAAAAGGAGT+GGG - Chr8:41175965-41175984 None:intergenic 45.0%
GTGGTGAATGATTGGGTAGA+GGG - Chr8:41175943-41175962 None:intergenic 45.0%
GTTGGCGATGGTGAATGATT+GGG - Chr8:41175921-41175940 None:intergenic 45.0%
TGAATGATTGGGTAGAGGGT+TGG - Chr8:41175939-41175958 None:intergenic 45.0%
TTGGCGATGGTGAATGATTG+GGG - Chr8:41175920-41175939 None:intergenic 45.0%
AACTAAGAAGAGCCACCACC+AGG - Chr8:41176315-41176334 None:intergenic 50.0%
ACAAAGCCAGCGAGCAAAAG+TGG + Chr8:41176088-41176107 MsG0880044387.01.T01:CDS 50.0%
AGGAGTGGGTGGTGAATGAT+TGG - Chr8:41175951-41175970 None:intergenic 50.0%
AGGCTTCGTAATACTCCTGG+TGG + Chr8:41176297-41176316 MsG0880044387.01.T01:CDS 50.0%
CAAAGCCAGCGAGCAAAAGT+GGG + Chr8:41176089-41176108 MsG0880044387.01.T01:CDS 50.0%
CTTCGTAATACTCCTGGTGG+TGG + Chr8:41176300-41176319 MsG0880044387.01.T01:CDS 50.0%
GAACACGGAACATGCCGTTA+GGG - Chr8:41176143-41176162 None:intergenic 50.0%
GAATGATTGGGGCGTGGAAT+GGG - Chr8:41175909-41175928 None:intergenic 50.0%
GGAGTGGGTGGTGAATGATT+GGG - Chr8:41175950-41175969 None:intergenic 50.0%
GGGTGTGGAAAGAAAAGGAG+TGG - Chr8:41175966-41175985 None:intergenic 50.0%
GGTGGTGAATGATTGGGTAG+AGG - Chr8:41175944-41175963 None:intergenic 50.0%
GGTTGGCGATGGTGAATGAT+TGG - Chr8:41175922-41175941 None:intergenic 50.0%
GTGGAAAGAAAAGGAGTGGG+TGG - Chr8:41175962-41175981 None:intergenic 50.0%
GTGGAATGGGATGGAAGATG+AGG - Chr8:41175896-41175915 None:intergenic 50.0%
TGAATGATTGGGGCGTGGAA+TGG - Chr8:41175910-41175929 None:intergenic 50.0%
! AGTGGGGGTGTGGAAAGAAA+AGG - Chr8:41175971-41175990 None:intergenic 50.0%
!! GTGAAGATGGATGGTGGAGT+GGG - Chr8:41176042-41176061 None:intergenic 50.0%
!! TGAAGATGGATGGTGGAGTG+GGG - Chr8:41176041-41176060 None:intergenic 50.0%
!!! CTTTTGCTCGCTGGCTTTGT+CGG - Chr8:41176088-41176107 None:intergenic 50.0%
!!! TTTTGCTCGCTGGCTTTGTC+GGG - Chr8:41176087-41176106 None:intergenic 50.0%
ACGGAACATGCCGTTAGGGA+GGG - Chr8:41176139-41176158 None:intergenic 55.0%
CGAACACGGAACATGCCGTT+AGG - Chr8:41176144-41176163 None:intergenic 55.0%
GAGACGGTGAAGATGGATGG+TGG - Chr8:41176048-41176067 None:intergenic 55.0%
GAGCAAAAGTGGGTCCACGA+AGG + Chr8:41176099-41176118 MsG0880044387.01.T01:CDS 55.0%
GATGGTGAATGATTGGGGCG+TGG - Chr8:41175915-41175934 None:intergenic 55.0%
TTTGTCGGGGGTTGAGGACT+TGG - Chr8:41176073-41176092 None:intergenic 55.0%
! ATGGTGGAGTGGGGTTTAGG+GGG - Chr8:41176032-41176051 None:intergenic 55.0%
! ATTGGGTAGAGGGTTGGCGA+TGG - Chr8:41175933-41175952 None:intergenic 55.0%
!! GATGGTGGAGTGGGGTTTAG+GGG - Chr8:41176033-41176052 None:intergenic 55.0%
!! GGATGGTGGAGTGGGGTTTA+GGG - Chr8:41176034-41176053 None:intergenic 55.0%
!! GGTGAAGATGGATGGTGGAG+TGG - Chr8:41176043-41176062 None:intergenic 55.0%
!! TGGATGGTGGAGTGGGGTTT+AGG - Chr8:41176035-41176054 None:intergenic 55.0%
!! TTTGCTCGCTGGCTTTGTCG+GGG - Chr8:41176086-41176105 None:intergenic 55.0%
CACGGAACATGCCGTTAGGG+AGG - Chr8:41176140-41176159 None:intergenic 60.0%
CTTGGCGGAGACGGTGAAGA+TGG - Chr8:41176055-41176074 None:intergenic 60.0%
GAAAGTGGAGAGTGGGGGTG+TGG - Chr8:41175981-41176000 None:intergenic 60.0%
GATTGGGGCGTGGAATGGGA+TGG - Chr8:41175905-41175924 None:intergenic 60.0%
GCGGAGACGGTGAAGATGGA+TGG - Chr8:41176051-41176070 None:intergenic 60.0%
GGAAGGATGGGGTGCGAAAG+TGG - Chr8:41175996-41176015 None:intergenic 60.0%
GGGGTGCGAAAGTGGAGAGT+GGG - Chr8:41175988-41176007 None:intergenic 60.0%
GGGTGCGAAAGTGGAGAGTG+GGG - Chr8:41175987-41176006 None:intergenic 60.0%
GGTGCGAAAGTGGAGAGTGG+GGG - Chr8:41175986-41176005 None:intergenic 60.0%
GGTTGAGGACTTGGCGGAGA+CGG - Chr8:41176064-41176083 None:intergenic 60.0%
TGGGGTGCGAAAGTGGAGAG+TGG - Chr8:41175989-41176008 None:intergenic 60.0%
TGGGGTTTAGGGGGGATGGT+GGG - Chr8:41176023-41176042 None:intergenic 60.0%
TGGTGGAGTGGGGTTTAGGG+GGG - Chr8:41176031-41176050 None:intergenic 60.0%
!! ATGGTGGGCGGAGGAAGGAT+GGG - Chr8:41176008-41176027 None:intergenic 60.0%
!! GTGGACCCACTTTTGCTCGC+TGG - Chr8:41176097-41176116 None:intergenic 60.0%
!! TTGCTCGCTGGCTTTGTCGG+GGG - Chr8:41176085-41176104 None:intergenic 60.0%
GGAGTGGGGTTTAGGGGGGA+TGG - Chr8:41176027-41176046 None:intergenic 65.0%
GTCGGGGGTTGAGGACTTGG+CGG - Chr8:41176070-41176089 None:intergenic 65.0%
GTGGGGTTTAGGGGGGATGG+TGG - Chr8:41176024-41176043 None:intergenic 65.0%
! TGGTGGGCGGAGGAAGGATG+GGG - Chr8:41176007-41176026 None:intergenic 65.0%
!! GATGGTGGGCGGAGGAAGGA+TGG - Chr8:41176009-41176028 None:intergenic 65.0%
!! GCTGGCTTTGTCGGGGGTTG+AGG - Chr8:41176079-41176098 None:intergenic 65.0%
!! GGTTTAGGGGGGATGGTGGG+CGG - Chr8:41176020-41176039 None:intergenic 65.0%
!! TTAGGGGGGATGGTGGGCGG+AGG - Chr8:41176017-41176036 None:intergenic 70.0%
!! GGGGGATGGTGGGCGGAGGA+AGG - Chr8:41176013-41176032 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 41175886 41176336 41175886 ID=MsG0880044387.01;Name=MsG0880044387.01
Chr8 mRNA 41175886 41176332 41175886 ID=MsG0880044387.01.T01;Parent=MsG0880044387.01;Name=MsG0880044387.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|148
Chr8 exon 41175886 41176332 41175886 ID=MsG0880044387.01.T01:exon:39379;Parent=MsG0880044387.01.T01
Chr8 CDS 41175886 41176332 41175886 ID=MsG0880044387.01.T01:cds;Parent=MsG0880044387.01.T01
Chr8 mRNA 41176150 41176332 41176150 ID=MsG0880044387.01.T02;Parent=MsG0880044387.01;Name=MsG0880044387.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|60
Chr8 exon 41176150 41176332 41176150 ID=MsG0880044387.01.T02:exon:39380;Parent=MsG0880044387.01.T02
Chr8 CDS 41176150 41176332 41176150 ID=MsG0880044387.01.T02:cds;Parent=MsG0880044387.01.T02
Chr8 mRNA 41176172 41176336 41176172 ID=MsG0880044387.01.T03;Parent=MsG0880044387.01;Name=MsG0880044387.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|54
Chr8 exon 41176172 41176336 41176172 ID=MsG0880044387.01.T03:exon:39381;Parent=MsG0880044387.01.T03
Chr8 CDS 41176172 41176336 41176172 ID=MsG0880044387.01.T03:cds;Parent=MsG0880044387.01.T03
Chr8 mRNA 41176268 41176336 41176268 ID=MsG0880044387.01.T04;Parent=MsG0880044387.01;Name=MsG0880044387.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|22
Chr8 exon 41176268 41176336 41176268 ID=MsG0880044387.01.T04:exon:39382;Parent=MsG0880044387.01.T04
Chr8 CDS 41176268 41176336 41176268 ID=MsG0880044387.01.T04:cds;Parent=MsG0880044387.01.T04
Gene Sequence

>MsG0880044387.01.T01

ATGTTTACCTCATCTTCCATCCCATTCCACGCCCCAATCATTCACCATCGCCAACCCTCTACCCAATCATTCACCACCCACTCCTTTTCTTTCCACACCCCCACTCTCCACTTTCGCACCCCATCCTTCCTCCGCCCACCATCCCCCCTAAACCCCACTCCACCATCCATCTTCACCGTCTCCGCCAAGTCCTCAACCCCCGACAAAGCCAGCGAGCAAAAGTGGGTCCACGAAGGTCTCATCACAGAATCCCTCCCTAACGGCATGTTCCGTGTTCGTCTCGATAATGAAGACCTTATCCTTGGATATATCTCTGGAAGGATCCGAAAGAATTACGTTCGGATCCTTCCTGGAGATAGAGTTAGAGTTGAAGTTAGTCGATATGATTCCTCCAAAGGACGCATCATTTATAGGCTTCGTAATACTCCTGGTGGTGGCTCTTCTTAG

>MsG0880044387.01.T03

ATGAAGACCTTATCCTTGGATATATCTCTGGAAGGATCCGAAAGAATTACGTTCGGATCCTTCCTGGAGATAGAGTTAGAGTTGAAGTTAGTCGATATGATTCCTCCAAAGGACGCATCATTTATAGGCTTCGTAATACTCCTGGTGGTGGCTCTTCTTAGTTAG

>MsG0880044387.01.T02

ATGTTCCGTGTTCGTCTCGATAATGAAGACCTTATCCTTGGATATATCTCTGGAAGGATCCGAAAGAATTACGTTCGGATCCTTCCTGGAGATAGAGTTAGAGTTGAAGTTAGTCGATATGATTCCTCCAAAGGACGCATCATTTATAGGCTTCGTAATACTCCTGGTGGTGGCTCTTCTTAG

>MsG0880044387.01.T04

ATGATTCCTCCAAAGGACGCATCATTTATAGGCTTCGTAATACTCCTGGTGGTGGCTCTTCTTAGTTAG

Protein sequence

>MsG0880044387.01.T01

MFTSSSIPFHAPIIHHRQPSTQSFTTHSFSFHTPTLHFRTPSFLRPPSPLNPTPPSIFTVSAKSSTPDKASEQKWVHEGLITESLPNGMFRVRLDNEDLILGYISGRIRKNYVRILPGDRVRVEVSRYDSSKGRIIYRLRNTPGGGSS*

>MsG0880044387.01.T03

MKTLSLDISLEGSERITFGSFLEIELELKLVDMIPPKDASFIGFVILLVVALLS*

>MsG0880044387.01.T02

MFRVRLDNEDLILGYISGRIRKNYVRILPGDRVRVEVSRYDSSKGRIIYRLRNTPGGGSS*

>MsG0880044387.01.T04

MIPPKDASFIGFVILLVVALLS*