AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005382.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005382.01.T01 MTR_1g098510 94.565 184 10 0 1 184 1 184 2.01e-128 358
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005382.01.T01 AT5G09270 55.789 95 37 2 90 184 19 108 4.28e-31 109
MsG0180005382.01.T01 AT5G09270 55.789 95 37 2 90 184 19 108 4.28e-31 109

Find 52 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCCTGCTGCAGGCTTTA+TGG 0.092653 1:+91092130 MsG0180005382.01.T01:CDS
CTCTTCTGCTACGGGCTTTA+TGG 0.095185 1:+91091959 MsG0180005382.01.T01:CDS
TATATGGCGGATTTGGTCTT+TGG 0.234560 1:+91092231 MsG0180005382.01.T01:CDS
ATTCTTGTATATGGCGGATT+TGG 0.303440 1:+91092224 MsG0180005382.01.T01:CDS
ATAGATATAGAAAGCTTCTT+AGG 0.309898 1:+91092261 MsG0180005382.01.T01:CDS
GGTTCCTATATTCTTGTATA+TGG 0.337081 1:+91092215 MsG0180005382.01.T01:CDS
TTCTGCCAAAGTGGTTGAAA+AGG 0.353945 1:+91092370 MsG0180005382.01.T01:CDS
CTTGGTCCTCACCAGTTTCC+AGG 0.356142 1:-91092097 None:intergenic
TGCAGGCTTTATGGATTCAT+TGG 0.369416 1:+91092139 MsG0180005382.01.T01:CDS
CAGAAACTTATCCTTATGTA+TGG 0.372733 1:+91092192 MsG0180005382.01.T01:CDS
TGGATAAGTGCTTCAGAACT+TGG 0.383586 1:-91091906 None:intergenic
TTATGGATTCATTGGAAAAT+CGG 0.384322 1:+91092147 MsG0180005382.01.T01:CDS
TGATTTGCCATGTCCTTGGC+TGG 0.408407 1:-91092029 None:intergenic
AAGAAGCTTTCTATATCTAT+AGG 0.413891 1:-91092258 None:intergenic
GTCGGTGCCCCTCCTGCTGC+AGG 0.419080 1:+91092122 MsG0180005382.01.T01:CDS
CAGGAGGGGCACCGACTACT+TGG 0.421600 1:-91092115 None:intergenic
AAGTTCTGCAGCACCTATCC+TGG 0.422828 1:+91092079 MsG0180005382.01.T01:CDS
AGGATAGGTGCTGCAGAACT+TGG 0.446859 1:-91092077 None:intergenic
AGTCGGTGGCTCTTCTGCTA+CGG 0.454743 1:+91091950 MsG0180005382.01.T01:CDS
GCAGCACCTATCCTGGAAAC+TGG 0.465240 1:+91092086 MsG0180005382.01.T01:CDS
GAAGCACTTATCCAGAAAAC+TGG 0.468163 1:+91091915 MsG0180005382.01.T01:CDS
GTCGGTGGCTCTTCTGCTAC+GGG 0.474768 1:+91091951 MsG0180005382.01.T01:CDS
TCCGCCATATACAAGAATAT+AGG 0.486205 1:-91092219 None:intergenic
TGGTTGATTTGCCATGTCCT+TGG 0.486730 1:-91092033 None:intergenic
ACTACGTGAGATGGTTGAAA+AGG 0.493284 1:+91092316 MsG0180005382.01.T01:CDS
AGCTTCTTAGGACAGAGGAC+AGG 0.494425 1:+91092273 MsG0180005382.01.T01:CDS
AGAAACTTATCCTTATGTAT+GGG 0.501079 1:+91092193 MsG0180005382.01.T01:CDS
TTCTAATAATTCTGCCAAAG+TGG 0.501415 1:+91092361 MsG0180005382.01.T01:CDS
TCTGCCAAAGTGGTTGAAAA+GGG 0.502981 1:+91092371 MsG0180005382.01.T01:CDS
ATATAGGAACCCCATACATA+AGG 0.508413 1:-91092203 None:intergenic
TCCTATATTCTTGTATATGG+CGG 0.519420 1:+91092218 MsG0180005382.01.T01:CDS
ATGTTGCAATGTAGGCTACT+TGG 0.523428 1:-91092417 None:intergenic
CATAAAGCCTGCAGCAGGAG+GGG 0.525453 1:-91092129 None:intergenic
TCCTCACCAGTTTCCAGGAT+AGG 0.526596 1:-91092092 None:intergenic
TCCATAAAGCCTGCAGCAGG+AGG 0.530529 1:-91092131 None:intergenic
TTAGGACAGAGGACAGGGTA+CGG 0.537011 1:+91092279 MsG0180005382.01.T01:CDS
GAATCCATAAAGCCTGCAGC+AGG 0.543830 1:-91092134 None:intergenic
TAGGACAGAGGACAGGGTAC+GGG 0.558112 1:+91092280 MsG0180005382.01.T01:CDS
GGAAGGAATCTCAGAAGATG+TGG 0.565631 1:-91092396 None:intergenic
GAAACTTATCCTTATGTATG+GGG 0.570070 1:+91092194 MsG0180005382.01.T01:CDS
TCAAGCAAGACTACGTGAGA+TGG 0.592566 1:+91092307 MsG0180005382.01.T01:CDS
CCATAAAGCCTGCAGCAGGA+GGG 0.618716 1:-91092130 None:intergenic
TAGCTGGAACACTCCAGCCA+AGG 0.621030 1:+91092016 MsG0180005382.01.T01:CDS
GAACACTCCAGCCAAGGACA+TGG 0.636957 1:+91092022 MsG0180005382.01.T01:CDS
GCTTCTTAGGACAGAGGACA+GGG 0.639564 1:+91092274 MsG0180005382.01.T01:CDS
ACCTATCCTGGAAACTGGTG+AGG 0.641675 1:+91092091 MsG0180005382.01.T01:CDS
TGCAATGTAGGCTACTTGGA+AGG 0.646398 1:-91092413 None:intergenic
ACTGGCGAGGATCAAGTAGT+CGG 0.654403 1:+91091933 MsG0180005382.01.T01:CDS
TAGAAAGCTTCTTAGGACAG+AGG 0.663471 1:+91092268 MsG0180005382.01.T01:CDS
ACTGGTGAGGACCAAGTAGT+CGG 0.664340 1:+91092104 MsG0180005382.01.T01:CDS
GGCGAGGATCAAGTAGTCGG+TGG 0.665719 1:+91091936 MsG0180005382.01.T01:CDS
ACTTATCCAGAAAACTGGCG+AGG 0.696112 1:+91091920 MsG0180005382.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGGATTCATTGAAAAAT+CGG + Chr1:91091976-91091995 MsG0180005382.01.T01:CDS 20.0%
! AAGAAGCTTTCTATATCTAT+AGG - Chr1:91092261-91092280 None:intergenic 25.0%
! AGAAACTTATCCTTATGTAT+GGG + Chr1:91092193-91092212 MsG0180005382.01.T01:CDS 25.0%
! ATAGATATAGAAAGCTTCTT+AGG + Chr1:91092261-91092280 MsG0180005382.01.T01:CDS 25.0%
! ATCAAAAAATCATAGATAGC+TGG + Chr1:91092000-91092019 MsG0180005382.01.T01:CDS 25.0%
! TTATGGATTCATTGGAAAAT+CGG + Chr1:91092147-91092166 MsG0180005382.01.T01:CDS 25.0%
CAGAAACTTATCCTTATGTA+TGG + Chr1:91092192-91092211 MsG0180005382.01.T01:CDS 30.0%
GAAACTTATCCTTATGTATG+GGG + Chr1:91092194-91092213 MsG0180005382.01.T01:CDS 30.0%
GGTTCCTATATTCTTGTATA+TGG + Chr1:91092215-91092234 MsG0180005382.01.T01:CDS 30.0%
TCCTATATTCTTGTATATGG+CGG + Chr1:91092218-91092237 MsG0180005382.01.T01:CDS 30.0%
TTCTAATAATTCTGCCAAAG+TGG + Chr1:91092361-91092380 MsG0180005382.01.T01:CDS 30.0%
!!! GCTTGTTGCTTTTCATTATT+TGG - Chr1:91092056-91092075 None:intergenic 30.0%
ATATAGGAACCCCATACATA+AGG - Chr1:91092206-91092225 None:intergenic 35.0%
TCCGCCATATACAAGAATAT+AGG - Chr1:91092222-91092241 None:intergenic 35.0%
!! ATTCTTGTATATGGCGGATT+TGG + Chr1:91092224-91092243 MsG0180005382.01.T01:CDS 35.0%
!!! GCTTGCTGCTTTTCATTATT+TGG - Chr1:91091885-91091904 None:intergenic 35.0%
ACTACGTGAGATGGTTGAAA+AGG + Chr1:91092316-91092335 MsG0180005382.01.T01:CDS 40.0%
TAGAAAGCTTCTTAGGACAG+AGG + Chr1:91092268-91092287 MsG0180005382.01.T01:CDS 40.0%
TCTGCCAAAGTGGTTGAAAA+GGG + Chr1:91092371-91092390 MsG0180005382.01.T01:CDS 40.0%
TGGATAAGTGCTTCAGAACT+TGG - Chr1:91091909-91091928 None:intergenic 40.0%
TTCTGCCAAAGTGGTTGAAA+AGG + Chr1:91092370-91092389 MsG0180005382.01.T01:CDS 40.0%
! GAAGCACTTATCCAGAAAAC+TGG + Chr1:91091915-91091934 MsG0180005382.01.T01:CDS 40.0%
! TGCAGGCTTTATGGATTCAT+TGG + Chr1:91092139-91092158 MsG0180005382.01.T01:CDS 40.0%
!! TATATGGCGGATTTGGTCTT+TGG + Chr1:91092231-91092250 MsG0180005382.01.T01:CDS 40.0%
ACTTATCCAGAAAACTGGCG+AGG + Chr1:91091920-91091939 MsG0180005382.01.T01:CDS 45.0%
GGAAGGAATCTCAGAAGATG+TGG - Chr1:91092399-91092418 None:intergenic 45.0%
TCAAGCAAGACTACGTGAGA+TGG + Chr1:91092307-91092326 MsG0180005382.01.T01:CDS 45.0%
! GGAACCCTTTTCAACCACTT+TGG - Chr1:91092378-91092397 None:intergenic 45.0%
!! TGGTTGATTTGCCATGTCCT+TGG - Chr1:91092036-91092055 None:intergenic 45.0%
AAGTTCTGCAGCACCTATCC+TGG + Chr1:91092079-91092098 MsG0180005382.01.T01:CDS 50.0%
ACCTATCCTGGAAACTGGTG+AGG + Chr1:91092091-91092110 MsG0180005382.01.T01:CDS 50.0%
ACTGGCGAGGATCAAGTAGT+CGG + Chr1:91091933-91091952 MsG0180005382.01.T01:CDS 50.0%
ACTGGTGAGGACCAAGTAGT+CGG + Chr1:91092104-91092123 MsG0180005382.01.T01:CDS 50.0%
AGCTTCTTAGGACAGAGGAC+AGG + Chr1:91092273-91092292 MsG0180005382.01.T01:CDS 50.0%
CTCTTCTGCTACGGGCTTTA+TGG + Chr1:91091959-91091978 MsG0180005382.01.T01:CDS 50.0%
GAATCCATAAAGCCTGCAGC+AGG - Chr1:91092137-91092156 None:intergenic 50.0%
GCTTCTTAGGACAGAGGACA+GGG + Chr1:91092274-91092293 MsG0180005382.01.T01:CDS 50.0%
TCCTCACCAGTTTCCAGGAT+AGG - Chr1:91092095-91092114 None:intergenic 50.0%
TTAGGACAGAGGACAGGGTA+CGG + Chr1:91092279-91092298 MsG0180005382.01.T01:CDS 50.0%
! AGGATAGGTGCTGCAGAACT+TGG - Chr1:91092080-91092099 None:intergenic 50.0%
! CTTGATCCTCGCCAGTTTTC+TGG - Chr1:91091929-91091948 None:intergenic 50.0%
! TGATTTGCCATGTCCTTGGC+TGG - Chr1:91092032-91092051 None:intergenic 50.0%
AGTCGGTGGCTCTTCTGCTA+CGG + Chr1:91091950-91091969 MsG0180005382.01.T01:CDS 55.0%
CATAAAGCCTGCAGCAGGAG+GGG - Chr1:91092132-91092151 None:intergenic 55.0%
CCATAAAGCCTGCAGCAGGA+GGG - Chr1:91092133-91092152 None:intergenic 55.0%
CTTGGTCCTCACCAGTTTCC+AGG - Chr1:91092100-91092119 None:intergenic 55.0%
GAACACTCCAGCCAAGGACA+TGG + Chr1:91092022-91092041 MsG0180005382.01.T01:CDS 55.0%
GCAGCACCTATCCTGGAAAC+TGG + Chr1:91092086-91092105 MsG0180005382.01.T01:CDS 55.0%
TAGCTGGAACACTCCAGCCA+AGG + Chr1:91092016-91092035 MsG0180005382.01.T01:CDS 55.0%
TAGGACAGAGGACAGGGTAC+GGG + Chr1:91092280-91092299 MsG0180005382.01.T01:CDS 55.0%
TCCATAAAGCCTGCAGCAGG+AGG - Chr1:91092134-91092153 None:intergenic 55.0%
CCCTCCTGCTGCAGGCTTTA+TGG + Chr1:91092130-91092149 MsG0180005382.01.T01:CDS 60.0%
GGCGAGGATCAAGTAGTCGG+TGG + Chr1:91091936-91091955 MsG0180005382.01.T01:CDS 60.0%
GTCGGTGGCTCTTCTGCTAC+GGG + Chr1:91091951-91091970 MsG0180005382.01.T01:CDS 60.0%
!! CAGGAGGGGCACCGACTACT+TGG - Chr1:91092118-91092137 None:intergenic 65.0%
! GTCGGTGCCCCTCCTGCTGC+AGG + Chr1:91092122-91092141 MsG0180005382.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 91091870 91092424 91091870 ID=MsG0180005382.01;Name=MsG0180005382.01
Chr1 mRNA 91091870 91092424 91091870 ID=MsG0180005382.01.T01;Parent=MsG0180005382.01;Name=MsG0180005382.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|184
Chr1 exon 91091870 91092424 91091870 ID=MsG0180005382.01.T01:exon:48129;Parent=MsG0180005382.01.T01
Chr1 CDS 91091870 91092424 91091870 ID=MsG0180005382.01.T01:cds;Parent=MsG0180005382.01.T01
Gene Sequence

>MsG0180005382.01.T01

ATGGCAAATCAACCAAATAATGAAAAGCAGCAAGCACCAAGTTCTGAAGCACTTATCCAGAAAACTGGCGAGGATCAAGTAGTCGGTGGCTCTTCTGCTACGGGCTTTATGGATTCATTGAAAAATCGGTATCAAAAAATCATAGATAGCTGGAACACTCCAGCCAAGGACATGGCAAATCAACCAAATAATGAAAAGCAACAAGCACCAAGTTCTGCAGCACCTATCCTGGAAACTGGTGAGGACCAAGTAGTCGGTGCCCCTCCTGCTGCAGGCTTTATGGATTCATTGGAAAATCGGTATAAAAAAATCAAAGAGCATGCAGAAACTTATCCTTATGTATGGGGTTCCTATATTCTTGTATATGGCGGATTTGGTCTTTGGTTTGCCTATAGATATAGAAAGCTTCTTAGGACAGAGGACAGGGTACGGGCACTTCAAGCAAGACTACGTGAGATGGTTGAAAAGGATAAGATTGTTGAAGCTGAAAATTCTAATAATTCTGCCAAAGTGGTTGAAAAGGGTTCCACATCTTCTGAGATTCCTTCCAAGTAG

Protein sequence

>MsG0180005382.01.T01

MANQPNNEKQQAPSSEALIQKTGEDQVVGGSSATGFMDSLKNRYQKIIDSWNTPAKDMANQPNNEKQQAPSSAAPILETGEDQVVGAPPAAGFMDSLENRYKKIKEHAETYPYVWGSYILVYGGFGLWFAYRYRKLLRTEDRVRALQARLREMVEKDKIVEAENSNNSAKVVEKGSTSSEIPSK*