AlfalfaGEDB | Msa0058130

M. sativa cultivar zhongmu-4 / Msa0058130

KEGG: K17338
GO: -
PFAM: PF03134.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058130	XP_003589468.1	93.824	340	11	1	1	330	1	340	0.0	576

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058130	sp\|Q8LE10\|HA22I_ARATH	52.439	328	118	7	1	326	1	292	9.42e-97	291

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058130	G7I756	93.824	340	11	1	1	330	1	340	0.0	576

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006840	Msa0058130	0.809564	1.778008e-50	-8.615850e-47
Msa0050530	Msa0058130	0.802505	5.435219e-49	-8.615850e-47
Msa0058130	Msa0063190	0.814714	1.338145e-51	-8.615850e-47
Msa0058130	Msa0091350	0.801217	9.994473e-49	-8.615850e-47
Msa0058130	Msa0100890	0.802517	5.404818e-49	-8.615850e-47
Msa0058130	Msa0108630	0.801746	7.783750e-49	-8.615850e-47
Msa0058130	Msa0109730	0.810974	8.827399e-51	-8.615850e-47
Msa0058130	Msa0125590	0.800022	1.751203e-48	-8.615850e-47
Msa0058130	Msa0127340	0.802122	6.515108e-49	-8.615850e-47
Msa0058130	Msa0128570	0.804548	2.048835e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058130	MtrunA17_Chr1g0156501	93.824	340	11	1	1	330	1	340	0.0	576
Msa0058130	MtrunA17_Chr3g0142571	59.517	331	86	6	1	330	1	284	6.54e-120	347
Msa0058130	MtrunA17_Chr1g0177871	44.211	190	87	2	1	190	1	171	4.24e-57	186
Msa0058130	MtrunA17_Chr7g0267131	50.345	145	72	0	1	145	1	145	1.29e-54	177
Msa0058130	MtrunA17_Chr5g0403731	32.632	95	62	1	15	109	37	129	1.82e-13	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0058130	AT5G42560.1	52.439	328	118	7	1	326	1	292	9.57e-98	291
Msa0058130	AT5G42560.3	51.582	316	115	7	13	326	2	281	2.62e-91	274
Msa0058130	AT5G42560.2	51.582	316	115	7	13	326	2	281	2.62e-91	274
Msa0058130	AT1G19950.1	47.353	340	139	6	1	328	1	312	5.49e-91	275
Msa0058130	AT1G75700.1	64.103	156	56	0	1	156	1	156	1.47e-78	238
Msa0058130	AT1G75700.2	64.103	156	56	0	1	156	1	156	1.47e-78	238
Msa0058130	AT1G75700.3	54.054	185	56	1	1	156	1	185	7.70e-72	222
Msa0058130	AT2G36020.1	50.955	157	77	0	1	157	1	157	4.68e-59	191
Msa0058130	AT4G36720.1	29.032	93	64	2	15	107	45	135	1.49e-11	63.2

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Find 120 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCGTTGTGTAACGGTTT+CGG	0.383090	1_2:+14398405	Msa0058130:three_prime_UTR
AAAGTAGCCCCTCCTGTTGC+AGG	0.293457	1_2:+14397997	Msa0058130:CDS
AAATGGAAAATCTCTTGTAA+AGG	0.557309	1_2:+14398257	Msa0058130:CDS
AAATTGAACAGCTTCGATTT+TGG	0.251242	1_2:+14396447	Msa0058130:CDS
AACATGAGCCAGAAATTGAT+CGG	0.579657	1_2:+14396927	Msa0058130:CDS
AACCAGAAGCCTCCTATAGC+TGG	0.489915	1_2:+14398087	Msa0058130:CDS
AACCAGCTATAGGAGGCTTC+TGG	0.393471	1_2:-14398089	None:intergenic
AACTTAGCTTCACAATACAT+TGG	0.528637	1_2:-14396733	None:intergenic
AACTTCAAGTCAGTTCCAAA+AGG	0.529032	1_2:+14397936	Msa0058130:CDS
AACTTGTGGTTCTGTAGCAG+CGG	0.597787	1_2:-14397884	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAAATCCAGAAGGAACTA+GGG	-	chr1_2:14395610-14395629	None:intergenic	30.0%
TTATTCAGCATAATGCTCAT+CGG	-	chr1_2:14395656-14395675	None:intergenic	30.0%
TAGTTTATGATGTAGTCCAT+TGG	+	chr1_2:14395953-14395972	Msa0058130:intron	30.0%
TGGTTTAATGATTAGCATTG+TGG	+	chr1_2:14396314-14396333	Msa0058130:intron	30.0%
AGTAGTTGAAAAGAATAAGC+CGG	+	chr1_2:14396424-14396443	Msa0058130:CDS	30.0%
ACAATATTGCATACCAATAC+TGG	-	chr1_2:14396475-14396494	None:intergenic	30.0%
ACTTAGCTTCACAATACATT+GGG	-	chr1_2:14396735-14396754	None:intergenic	30.0%
AACTTAGCTTCACAATACAT+TGG	-	chr1_2:14396736-14396755	None:intergenic	30.0%
AATGTATTGTGAAGCTAAGT+TGG	+	chr1_2:14396735-14396754	Msa0058130:CDS	30.0%
GTAAAGTTTACCTTTGTGTT+AGG	+	chr1_2:14396859-14396878	Msa0058130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	14395147	14398659	14395147	ID=Msa0058130;Name=Msa0058130
chr1_2	mRNA	14395147	14398659	14395147	ID=Msa0058130-mRNA-1;Parent=Msa0058130;Name=Msa0058130-mRNA-1;_AED=0.02;_eAED=0.02;_QI=317\|1\|1\|1\|1\|1\|7\|303\|330
chr1_2	exon	14395147	14395454	14395147	ID=Msa0058130-mRNA-1:exon:5768;Parent=Msa0058130-mRNA-1
chr1_2	exon	14396139	14396179	14396139	ID=Msa0058130-mRNA-1:exon:5769;Parent=Msa0058130-mRNA-1
chr1_2	exon	14396382	14396480	14396382	ID=Msa0058130-mRNA-1:exon:5770;Parent=Msa0058130-mRNA-1
chr1_2	exon	14396584	14396640	14396584	ID=Msa0058130-mRNA-1:exon:5771;Parent=Msa0058130-mRNA-1
chr1_2	exon	14396729	14396792	14396729	ID=Msa0058130-mRNA-1:exon:5772;Parent=Msa0058130-mRNA-1
chr1_2	exon	14396881	14397090	14396881	ID=Msa0058130-mRNA-1:exon:5773;Parent=Msa0058130-mRNA-1
chr1_2	exon	14397826	14398659	14397826	ID=Msa0058130-mRNA-1:exon:5774;Parent=Msa0058130-mRNA-1
chr1_2	five_prime_UTR	14395147	14395454	14395147	ID=Msa0058130-mRNA-1:five_prime_utr;Parent=Msa0058130-mRNA-1
chr1_2	five_prime_UTR	14396139	14396147	14396139	ID=Msa0058130-mRNA-1:five_prime_utr;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14396148	14396179	14396148	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14396382	14396480	14396382	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14396584	14396640	14396584	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14396729	14396792	14396729	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14396881	14397090	14396881	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	CDS	14397826	14398356	14397826	ID=Msa0058130-mRNA-1:cds;Parent=Msa0058130-mRNA-1
chr1_2	three_prime_UTR	14398357	14398659	14398357	ID=Msa0058130-mRNA-1:three_prime_utr;Parent=Msa0058130-mRNA-1

Gene Sequence

>Msa0058130

ATGATAGGATCCTTTCTTACCTGGGCGCTTGTGAGTGTTTTTGGCTATGCCTATCCAGCGTATGAATGCTATAAAGTAGTTGAAAAGAATAAGCCGGAAATTGAACAGCTTCGATTTTGGTGCCAGTATTGGATTTTGGTTGCTGTTATGACAGTGTGTGAGAGATTTGGAGATACTTTTGTATCATGGGTCCCAATGTATTGTGAAGCTAAGTTGGCATTTTTCATATTTTTGTGGTACCCCAAAACAAAGGGAACAACATATGTATATGATTCCTTCTTTAAACCATATGTTGCAAAACATGAGCCAGAAATTGATCGGAGCTTGTCGGAACTAAGGACAAGGGCGGGAGATTATGTAGTTTTGTATTCTCAAAGAGCTGTTAGTTACGGTCAGACTAGAATATACGACATTTTGAAGTTTGTTTCAGCACAATCAACACCAGCACCTCAGTCCGCTCAGCAACGACCGGGACCGGGTGTGAGAGTTCGTAAACCTGCACCTGCCAAGGGCCAACCAGCCGCTGCTACAGAACCACAAGTTGAAGAACCCCCATCTCCTGCCTCTAGCACAACTTCAAGTCAGTTCCAAAAGGAAGTAAAAGAGGAGTTATTGGATTCTCCACAAGCTCCTAAAGTAGCCCCTCCTGTTGCAGGTTTAAACAAACAGAAGTCCTCTGTAGCTGCTTTAAATAACCAGAAGCCTCCTGTAGCTGCTTTAAATAACCAGAAGCCTCCTATAGCTGGTTTAACTACTCAAAAATCTTCTGTAGCTGCTTTACATACCCAGAAGTCTAATCCTTCATCTGAAACAATTATCATATCTTCTTCATCAACAGAGTCAGAACCAAAGAATATTGAATCAACAGCAACATCCTCCTCTTCAGCAACTGAAAATGGAAAATCTCTTGTAAAGGAGGAGACCAGAATGGAAGAAAGTATTCGGGTTACACGGGGAAGACTAAGAAAAACCAGATCAGTAGGAACCCAGTAG

Protein sequence

>Msa0058130

MIGSFLTWALVSVFGYAYPAYECYKVVEKNKPEIEQLRFWCQYWILVAVMTVCERFGDTFVSWVPMYCEAKLAFFIFLWYPKTKGTTYVYDSFFKPYVAKHEPEIDRSLSELRTRAGDYVVLYSQRAVSYGQTRIYDILKFVSAQSTPAPQSAQQRPGPGVRVRKPAPAKGQPAAATEPQVEEPPSPASSTTSSQFQKEVKEELLDSPQAPKVAPPVAGLNKQKSSVAALNNQKPPVAALNNQKPPIAGLTTQKSSVAALHTQKSNPSSETIIISSSSTESEPKNIESTATSSSSATENGKSLVKEETRMEESIRVTRGRLRKTRSVGTQ*