AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0856610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0856610 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0856610 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0856610 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0856610 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0856610 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0856610 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0856610 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0856610 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0856610 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0856610 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0856610 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0856610 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0856610 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0856610 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0856610 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0856610 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0856610 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0856610 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0856610 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0856610 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0856610 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0856610 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0856610 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0856610 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0856610 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0856610 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0856610 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0856610 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0856610 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0856610 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0856610 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0856610 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCTCTAACCAATCTT+TGG 0.237540 5_4:-51391398 None:intergenic
TTCTTCACTCCTCCGGTTGC+CGG 0.280449 5_4:-51391285 None:intergenic
TCACGAAGAGCGACTGTTCC+TGG 0.301860 5_4:-51391324 None:intergenic
GCAGCTGAAGCTTATCTTGT+TGG 0.346066 5_4:+51391480 Msa0856610:CDS
AGCTGATTTCCGAGCGGCTT+TGG 0.356003 5_4:-51391262 None:intergenic
GAAGCTTCCTTTCCAAAGAT+TGG 0.376805 5_4:+51391386 Msa0856610:CDS
CGGAAATCAGCTCCGGCAAC+CGG 0.396553 5_4:+51391273 Msa0856610:CDS
GAGCGGAAACAGCGCTACTC+TGG 0.433957 5_4:-51391449 None:intergenic
TTGGTGGCGAGCTGTTTCCT+TGG 0.436325 5_4:-51391243 None:intergenic
AAGCCCTCTCGCCTCTGATT+CGG 0.461144 5_4:-51391581 None:intergenic
ATGTGGCTTCTTCACTCCTC+CGG 0.485740 5_4:-51391292 None:intergenic
AGCCGCTCGGAAATCAGCTC+CGG 0.492100 5_4:+51391266 Msa0856610:CDS
AGCATGAATAGCACAAAGAT+TGG 0.492949 5_4:-51391517 None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG 0.495094 5_4:-51391259 None:intergenic
CGAGCAAGTTGAATATCCTT+AGG 0.498424 5_4:-51391558 None:intergenic
AAACAGCGCTACTCTGGAAT+CGG 0.503171 5_4:-51391443 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 5_4:+51391542 Msa0856610:CDS
AGAGCACAGAGCTTCTCATA+AGG 0.533669 5_4:+51391364 Msa0856610:CDS
CAACTTGCTCGCCGAATCAG+AGG 0.534605 5_4:+51391570 Msa0856610:CDS
ATTGGTTAGAGAAATTGCTC+AGG 0.542546 5_4:+51391404 Msa0856610:CDS
TCTCTAACCAATCTTTGGAA+AGG 0.542941 5_4:-51391393 None:intergenic
AGCTCGCCACCAAAGCCGCT+CGG 0.547091 5_4:+51391253 Msa0856610:CDS
TTCAGCTGCTTCTTGAAGAG+CGG 0.576635 5_4:-51391466 None:intergenic
TCGCCGAATCAGAGGCGAGA+GGG 0.598725 5_4:+51391578 Msa0856610:CDS
AAATCAGCTCCGGCAACCGG+AGG 0.609979 5_4:+51391276 Msa0856610:CDS
AAGCCACATAGATTCCGTCC+AGG 0.611045 5_4:+51391306 Msa0856610:CDS
CAAACAGCAAGAAAATCCAC+CGG 0.620184 5_4:+51391210 Msa0856610:CDS
CTCGCCGAATCAGAGGCGAG+AGG 0.633846 5_4:+51391577 Msa0856610:CDS
GTTCCTGGACGGAATCTATG+TGG 0.636510 5_4:-51391309 None:intergenic
GAAGAGCGACTGTTCCTGGA+CGG 0.668213 5_4:-51391320 None:intergenic
CCACCGGAGGAAAAGCACCA+AGG 0.683879 5_4:+51391226 Msa0856610:CDS
ACAGCAAGAAAATCCACCGG+AGG 0.684797 5_4:+51391213 Msa0856610:CDS
TGCCGGAGCTGATTTCCGAG+CGG 0.724331 5_4:-51391268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAATTTCTCTAACCAATCTT+TGG - chr5_4:51391401-51391420 None:intergenic 30.0%
!!! GGATTTTCTTGCTGTTTGTT+TGG - chr5_4:51391208-51391227 None:intergenic 35.0%
! TCTCTAACCAATCTTTGGAA+AGG - chr5_4:51391396-51391415 None:intergenic 35.0%
ATTGGTTAGAGAAATTGCTC+AGG + chr5_4:51391404-51391423 Msa0856610:CDS 35.0%
AGCATGAATAGCACAAAGAT+TGG - chr5_4:51391520-51391539 None:intergenic 35.0%
GAGAGTTACTATTATGCCTA+AGG + chr5_4:51391542-51391561 Msa0856610:CDS 35.0%
CAAACAGCAAGAAAATCCAC+CGG + chr5_4:51391210-51391229 Msa0856610:CDS 40.0%
GAAGCTTCCTTTCCAAAGAT+TGG + chr5_4:51391386-51391405 Msa0856610:CDS 40.0%
! CGAGCAAGTTGAATATCCTT+AGG - chr5_4:51391561-51391580 None:intergenic 40.0%
!!! TTTCCTTGGTGCTTTTCCTC+CGG - chr5_4:51391232-51391251 None:intergenic 45.0%
AGAGCACAGAGCTTCTCATA+AGG + chr5_4:51391364-51391383 Msa0856610:CDS 45.0%
!! AAACAGCGCTACTCTGGAAT+CGG - chr5_4:51391446-51391465 None:intergenic 45.0%
TTCAGCTGCTTCTTGAAGAG+CGG - chr5_4:51391469-51391488 None:intergenic 45.0%
GCAGCTGAAGCTTATCTTGT+TGG + chr5_4:51391480-51391499 Msa0856610:CDS 45.0%
ACAGCAAGAAAATCCACCGG+AGG + chr5_4:51391213-51391232 Msa0856610:CDS 50.0%
ATGTGGCTTCTTCACTCCTC+CGG - chr5_4:51391295-51391314 None:intergenic 50.0%
AAGCCACATAGATTCCGTCC+AGG + chr5_4:51391306-51391325 Msa0856610:CDS 50.0%
GTTCCTGGACGGAATCTATG+TGG - chr5_4:51391312-51391331 None:intergenic 50.0%
!! TTGGTGGCGAGCTGTTTCCT+TGG - chr5_4:51391246-51391265 None:intergenic 55.0%
! TGATTTCCGAGCGGCTTTGG+TGG - chr5_4:51391262-51391281 None:intergenic 55.0%
! AGCTGATTTCCGAGCGGCTT+TGG - chr5_4:51391265-51391284 None:intergenic 55.0%
TTCTTCACTCCTCCGGTTGC+CGG - chr5_4:51391288-51391307 None:intergenic 55.0%
GAAGAGCGACTGTTCCTGGA+CGG - chr5_4:51391323-51391342 None:intergenic 55.0%
TCACGAAGAGCGACTGTTCC+TGG - chr5_4:51391327-51391346 None:intergenic 55.0%
! CAACTTGCTCGCCGAATCAG+AGG + chr5_4:51391570-51391589 Msa0856610:CDS 55.0%
AAGCCCTCTCGCCTCTGATT+CGG - chr5_4:51391584-51391603 None:intergenic 55.0%
!!! CCTTGGTGCTTTTCCTCCGG+TGG - chr5_4:51391229-51391248 None:intergenic 60.0%
!! CCACCGGAGGAAAAGCACCA+AGG + chr5_4:51391226-51391245 Msa0856610:CDS 60.0%
AGCCGCTCGGAAATCAGCTC+CGG + chr5_4:51391266-51391285 Msa0856610:CDS 60.0%
! TGCCGGAGCTGATTTCCGAG+CGG - chr5_4:51391271-51391290 None:intergenic 60.0%
CGGAAATCAGCTCCGGCAAC+CGG + chr5_4:51391273-51391292 Msa0856610:CDS 60.0%
AAATCAGCTCCGGCAACCGG+AGG + chr5_4:51391276-51391295 Msa0856610:CDS 60.0%
GAGCGGAAACAGCGCTACTC+TGG - chr5_4:51391452-51391471 None:intergenic 60.0%
TCGCCGAATCAGAGGCGAGA+GGG + chr5_4:51391578-51391597 Msa0856610:CDS 60.0%
AGCTCGCCACCAAAGCCGCT+CGG + chr5_4:51391253-51391272 Msa0856610:CDS 65.0%
CTCGCCGAATCAGAGGCGAG+AGG + chr5_4:51391577-51391596 Msa0856610:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 51391195 51391605 51391195 ID=Msa0856610;Name=Msa0856610
chr5_4 mRNA 51391195 51391605 51391195 ID=Msa0856610-mRNA-1;Parent=Msa0856610;Name=Msa0856610-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|136
chr5_4 exon 51391195 51391605 51391195 ID=Msa0856610-mRNA-1:exon:16058;Parent=Msa0856610-mRNA-1
chr5_4 CDS 51391195 51391605 51391195 ID=Msa0856610-mRNA-1:cds;Parent=Msa0856610-mRNA-1
Gene Sequence

>Msa0856610

ATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCACCAAGGAAACAGCTCGCCACCAAAGCCGCTCGGAAATCAGCTCCGGCAACCGGAGGAGTGAAGAAGCCACATAGATTCCGTCCAGGAACAGTCGCTCTTCGTGAAATCAGAAAGTATCAGAAGAGCACAGAGCTTCTCATAAGGAAGCTTCCTTTCCAAAGATTGGTTAGAGAAATTGCTCAGGATTTCAAAACTGATCTCCGATTCCAGAGTAGCGCTGTTTCCGCTCTTCAAGAAGCAGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACCAATCTTTGTGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCTCGCCGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa0856610

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*