AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0684750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684750 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0684750 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0684750 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0684750 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0684750 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0684750 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0684750 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0684750 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0684750 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0684750 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0684750 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0684750 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0684750 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0684750 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0684750 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0684750 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684750 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0684750 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0684750 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0684750 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0684750 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0684750 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0684750 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0684750 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0684750 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0684750 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0684750 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0684750 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0684750 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0684750 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0684750 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0684750 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 32 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCAAGCTGAATATCTT+TGG 0.249802 4_4:-48386078 None:intergenic
TTCACGGAGTGCGACGGTTC+CGG 0.309270 4_4:-48385844 None:intergenic
TCACGGAGTGCGACGGTTCC+GGG 0.327175 4_4:-48385843 None:intergenic
TTCTTCACTCCGCCGGTGGC+TGG 0.345560 4_4:-48385804 None:intergenic
CTTGCAAGCTGAATATCTTT+GGG 0.357406 4_4:-48386077 None:intergenic
GGTGGCTGGAGCGGATTTGC+GGG 0.370843 4_4:-48385790 None:intergenic
CACGAACGAGACGTTGGAAT+GGG 0.414135 4_4:-48385911 None:intergenic
GAGCAGAAACAGCGCTACTC+TGG 0.427137 4_4:-48385968 None:intergenic
CGCAAATCCGCTCCAGCCAC+CGG 0.436359 4_4:+48385792 Msa0684750:CDS
TTTGGTATTTGCGGATTTCA+CGG 0.451483 4_4:-48385860 None:intergenic
CACTCCGCCGGTGGCTGGAG+CGG 0.457833 4_4:-48385799 None:intergenic
CGGTGGCTGGAGCGGATTTG+CGG 0.465165 4_4:-48385791 None:intergenic
TCACGAACGAGACGTTGGAA+TGG 0.487442 4_4:-48385912 None:intergenic
GCTGCTGAAGCTTATCTCGT+TGG 0.507565 4_4:+48385999 Msa0684750:CDS
AGCATGAATAGCGCAAAGAT+TGG 0.509783 4_4:-48386036 None:intergenic
CGACGGTTCCGGGACGGAAA+CGG 0.513929 4_4:-48385833 None:intergenic
AAGCCTCACCGTTTCCGTCC+CGG 0.527320 4_4:+48385825 Msa0684750:CDS
TCGGGGAGCTTTGCCGCCGG+TGG 0.528357 4_4:-48385745 None:intergenic
AAACAGCGCTACTCTGGAAA+CGG 0.532210 4_4:-48385962 None:intergenic
GCTACTCTGGAAACGGAGAT+CGG 0.538527 4_4:-48385955 None:intergenic
CGATTTCACGAACGAGACGT+TGG 0.545134 4_4:-48385917 None:intergenic
GTGAGGCTTCTTCACTCCGC+CGG 0.560763 4_4:-48385811 None:intergenic
GGCTGGAGCGGATTTGCGGG+CGG 0.566126 4_4:-48385787 None:intergenic
TCTCGTTCGTGAAATCGCTC+AGG 0.574609 4_4:+48385923 Msa0684750:CDS
AGGCTTCTTCACTCCGCCGG+TGG 0.575034 4_4:-48385808 None:intergenic
CAAACAGCTCGCAAATCCAC+CGG 0.575165 4_4:+48385729 Msa0684750:CDS
GCGGATTTCACGGAGTGCGA+CGG 0.595387 4_4:-48385850 None:intergenic
GGAGTGCGACGGTTCCGGGA+CGG 0.649162 4_4:-48385839 None:intergenic
AAATCCGCTCCAGCCACCGG+CGG 0.651593 4_4:+48385795 Msa0684750:CDS
CAGCTTGCAAGAAGAATCAG+AGG 0.660238 4_4:+48386089 Msa0684750:CDS
ACAGCTCGCAAATCCACCGG+CGG 0.668402 4_4:+48385732 Msa0684750:CDS
GTTCCGGGACGGAAACGGTG+AGG 0.691461 4_4:-48385828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTTGGTATTTGCGGATTTCA+CGG - chr4_4:48385863-48385882 None:intergenic 35.0%
!!! CTGTGCTTTTTTGGTATTTG+CGG - chr4_4:48385872-48385891 None:intergenic 35.0%
CTTGCAAGCTGAATATCTTT+GGG - chr4_4:48386080-48386099 None:intergenic 35.0%
TCTTGCAAGCTGAATATCTT+TGG - chr4_4:48386081-48386100 None:intergenic 35.0%
!! TTTTGTGGCGAGTTGTTTTC+GGG - chr4_4:48385766-48385785 None:intergenic 40.0%
!! CTTTTGTGGCGAGTTGTTTT+CGG - chr4_4:48385767-48385786 None:intergenic 40.0%
!!! TGAGAAGCTCTGTGCTTTTT+TGG - chr4_4:48385881-48385900 None:intergenic 40.0%
AGCATGAATAGCGCAAAGAT+TGG - chr4_4:48386039-48386058 None:intergenic 40.0%
!! TTTGTGGCGAGTTGTTTTCG+GGG - chr4_4:48385765-48385784 None:intergenic 45.0%
! GACGTTGGAATGGGAGTTTT+CGG - chr4_4:48385905-48385924 None:intergenic 45.0%
! AAACAGCGCTACTCTGGAAA+CGG - chr4_4:48385965-48385984 None:intergenic 45.0%
CAGCTTGCAAGAAGAATCAG+AGG + chr4_4:48386089-48386108 Msa0684750:CDS 45.0%
CAAACAGCTCGCAAATCCAC+CGG + chr4_4:48385729-48385748 Msa0684750:CDS 50.0%
CACGAACGAGACGTTGGAAT+GGG - chr4_4:48385914-48385933 None:intergenic 50.0%
TCACGAACGAGACGTTGGAA+TGG - chr4_4:48385915-48385934 None:intergenic 50.0%
CGATTTCACGAACGAGACGT+TGG - chr4_4:48385920-48385939 None:intergenic 50.0%
TCTCGTTCGTGAAATCGCTC+AGG + chr4_4:48385923-48385942 Msa0684750:CDS 50.0%
! GCTACTCTGGAAACGGAGAT+CGG - chr4_4:48385958-48385977 None:intergenic 50.0%
GCTGCTGAAGCTTATCTCGT+TGG + chr4_4:48385999-48386018 Msa0684750:CDS 50.0%
GAGCAGAAACAGCGCTACTC+TGG - chr4_4:48385971-48385990 None:intergenic 55.0%
ACAGCTCGCAAATCCACCGG+CGG + chr4_4:48385732-48385751 Msa0684750:CDS 60.0%
!! TTTTCGGGGAGCTTTGCCGC+CGG - chr4_4:48385751-48385770 None:intergenic 60.0%
! GGATTTGCGGGCGGCTTTTG+TGG - chr4_4:48385781-48385800 None:intergenic 60.0%
GTGAGGCTTCTTCACTCCGC+CGG - chr4_4:48385814-48385833 None:intergenic 60.0%
AAGCCTCACCGTTTCCGTCC+CGG + chr4_4:48385825-48385844 Msa0684750:CDS 60.0%
TTCACGGAGTGCGACGGTTC+CGG - chr4_4:48385847-48385866 None:intergenic 60.0%
GCGGATTTCACGGAGTGCGA+CGG - chr4_4:48385853-48385872 None:intergenic 60.0%
! GGTGGCTGGAGCGGATTTGC+GGG - chr4_4:48385793-48385812 None:intergenic 65.0%
! CGGTGGCTGGAGCGGATTTG+CGG - chr4_4:48385794-48385813 None:intergenic 65.0%
CGCAAATCCGCTCCAGCCAC+CGG + chr4_4:48385792-48385811 Msa0684750:CDS 65.0%
AAATCCGCTCCAGCCACCGG+CGG + chr4_4:48385795-48385814 Msa0684750:CDS 65.0%
TTCTTCACTCCGCCGGTGGC+TGG - chr4_4:48385807-48385826 None:intergenic 65.0%
AGGCTTCTTCACTCCGCCGG+TGG - chr4_4:48385811-48385830 None:intergenic 65.0%
GTTCCGGGACGGAAACGGTG+AGG - chr4_4:48385831-48385850 None:intergenic 65.0%
CGACGGTTCCGGGACGGAAA+CGG - chr4_4:48385836-48385855 None:intergenic 65.0%
TCACGGAGTGCGACGGTTCC+GGG - chr4_4:48385846-48385865 None:intergenic 65.0%
! GGCTGGAGCGGATTTGCGGG+CGG - chr4_4:48385790-48385809 None:intergenic 70.0%
GGAGTGCGACGGTTCCGGGA+CGG - chr4_4:48385842-48385861 None:intergenic 70.0%
TCGGGGAGCTTTGCCGCCGG+TGG - chr4_4:48385748-48385767 None:intergenic 75.0%
CACTCCGCCGGTGGCTGGAG+CGG - chr4_4:48385802-48385821 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 48385714 48386124 48385714 ID=Msa0684750;Name=Msa0684750
chr4_4 mRNA 48385714 48386124 48385714 ID=Msa0684750-mRNA-1;Parent=Msa0684750;Name=Msa0684750-mRNA-1;_AED=0.41;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|136
chr4_4 exon 48385714 48386124 48385714 ID=Msa0684750-mRNA-1:exon:12008;Parent=Msa0684750-mRNA-1
chr4_4 CDS 48385714 48386124 48385714 ID=Msa0684750-mRNA-1:cds;Parent=Msa0684750-mRNA-1
Gene Sequence

>Msa0684750

ATGGCGCGTACAAAGCAAACAGCTCGCAAATCCACCGGCGGCAAAGCTCCCCGAAAACAACTCGCCACAAAAGCCGCCCGCAAATCCGCTCCAGCCACCGGCGGAGTGAAGAAGCCTCACCGTTTCCGTCCCGGAACCGTCGCACTCCGTGAAATCCGCAAATACCAAAAAAGCACAGAGCTTCTCATCCGAAAACTCCCATTCCAACGTCTCGTTCGTGAAATCGCTCAGGATTTCAAAACCGATCTCCGTTTCCAGAGTAGCGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTCGTTGGTTTGTTTGAAGATACCAATCTTTGCGCTATTCATGCTAAGAGAGTTACGATTATGCCCAAAGATATTCAGCTTGCAAGAAGAATCAGAGGCGAGAGAGCTTAA

Protein sequence

>Msa0684750

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*