AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0029020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0029020 MtrunA17_Chr1g0185661 93.333 30 2 0 3 32 655 684 1.28e-11 61.2
Msa0029020 MtrunA17_Chr1g0185661 93.103 29 2 0 4 32 5 33 2.40e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTCAACAATCTTTC+AGG 0.152886 1_1:-54143559 Msa0029020:CDS
ATCTCGGTGGCCAATTCCTC+TGG 0.231618 1_1:-54143430 Msa0029020:CDS
TGTAATGAAGTATTGAAAAT+TGG 0.259063 1_1:+54143613 None:intergenic
CGCAATCAACCCAGAGGAAT+TGG 0.261348 1_1:+54143420 None:intergenic
TTATCAATCCAATCCTCTAA+TGG 0.328408 1_1:-54143206 Msa0029020:CDS
GCTGGGAGAACTAGGGCTTT+TGG 0.331746 1_1:+54143536 None:intergenic
TCCTGGGTTGAAGCCATTAG+AGG 0.369624 1_1:+54143193 None:intergenic
ATCACCACAGCCGTCGGAAT+TGG 0.371692 1_1:+54143334 None:intergenic
CGGAATTGGCGAGGGAGCAT+TGG 0.411697 1_1:+54143348 None:intergenic
CAGGTGTTGTTGTTGCAGCC+AGG 0.418057 1_1:+54143293 None:intergenic
GGAATTGGCGAGGGAGCATT+GGG 0.432376 1_1:+54143349 None:intergenic
GCTGGTGGCGGTGTGGGTGA+TGG 0.441452 1_1:+54143250 None:intergenic
TGGGCTGAGCGACACCGTGC+AGG 0.443405 1_1:+54143368 None:intergenic
TGTGGGTGATGGAATTGTCC+GGG 0.446715 1_1:+54143261 None:intergenic
TTGTTGTTGCAGCCAGGCTT+TGG 0.449409 1_1:+54143299 None:intergenic
GGTTGAAGCCATTAGAGGAT+TGG 0.451183 1_1:+54143198 None:intergenic
GTGTGGGTGATGGAATTGTC+CGG 0.454258 1_1:+54143260 None:intergenic
CTCGCCAATTCCGACGGCTG+TGG 0.458653 1_1:-54143338 Msa0029020:CDS
GAAACTGCGACATTTCGTCC+TGG 0.459544 1_1:+54143176 None:intergenic
AGCAAGTTCACCGCTGGTGG+CGG 0.469786 1_1:+54143238 None:intergenic
TTCACCGCTGGTGGCGGTGT+GGG 0.483515 1_1:+54143244 None:intergenic
TGTTTCTCATCACCAAAGCC+TGG 0.494972 1_1:-54143311 Msa0029020:CDS
TCCTCTAATGGCTTCAACCC+AGG 0.495018 1_1:-54143194 Msa0029020:CDS
CTCAACGTCATCGTCGATCT+CGG 0.497477 1_1:-54143446 Msa0029020:CDS
AAACTGCGACATTTCGTCCT+GGG 0.498880 1_1:+54143177 None:intergenic
AAGTTGGTGCACATGAGAAT+AGG 0.499306 1_1:+54143147 None:intergenic
TCTCGGTGGCCAATTCCTCT+GGG 0.509201 1_1:-54143429 Msa0029020:CDS
ACACCGTGCAGGGCGGTATG+TGG 0.520199 1_1:+54143379 None:intergenic
GAAAGATTGTTGAGAGAAAG+TGG 0.530349 1_1:+54143562 None:intergenic
ATTGTCCGGGGTGACACCAC+AGG 0.542125 1_1:+54143274 None:intergenic
GGGCTGAGCGACACCGTGCA+GGG 0.548753 1_1:+54143369 None:intergenic
ATCTTCAGCAAGTTCACCGC+TGG 0.549080 1_1:+54143232 None:intergenic
GTATTGAAAATTGGAAGCCA+TGG 0.560507 1_1:+54143622 None:intergenic
TTCAGCAAGTTCACCGCTGG+TGG 0.561694 1_1:+54143235 None:intergenic
GTTCACCGCTGGTGGCGGTG+TGG 0.562370 1_1:+54143243 None:intergenic
AGATCGACGATGACGTTGAG+TGG 0.568219 1_1:+54143449 None:intergenic
TGCTCCCTCGCCAATTCCGA+CGG 0.580900 1_1:-54143344 Msa0029020:CDS
CACAGCCGTCGGAATTGGCG+AGG 0.587987 1_1:+54143339 None:intergenic
CCTCCACATACCGCCCTGCA+CGG 0.588163 1_1:-54143382 Msa0029020:CDS
ACAGCCGTCGGAATTGGCGA+GGG 0.612804 1_1:+54143340 None:intergenic
CAACACCTGTGGTGTCACCC+CGG 0.614141 1_1:-54143279 Msa0029020:CDS
CCGTGCAGGGCGGTATGTGG+AGG 0.617989 1_1:+54143382 None:intergenic
ATCACCCACACCGCCACCAG+CGG 0.641652 1_1:-54143248 Msa0029020:CDS
GATCGACGATGACGTTGAGT+GGG 0.647965 1_1:+54143450 None:intergenic
GAAACAATCACCACAGCCGT+CGG 0.679467 1_1:+54143328 None:intergenic
GTGGGTGATGGAATTGTCCG+GGG 0.682973 1_1:+54143262 None:intergenic
GGTATGTGGAGGAAATGTAG+TGG 0.686881 1_1:+54143393 None:intergenic
GGCTGCAACAACAACACCTG+TGG 0.687479 1_1:-54143290 Msa0029020:CDS
AACGTCATCGTCGATCTCGG+TGG 0.719707 1_1:-54143443 Msa0029020:CDS
CTGAGCGACACCGTGCAGGG+CGG 0.729304 1_1:+54143372 None:intergenic
AAAGATTGTTGAGAGAAAGT+GGG 0.736615 1_1:+54143563 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTAATGAAGTATTGAAAAT+TGG + chr1_1:54143148-54143167 None:intergenic 20.0%
! AATGAAGAAAAATAGGAGTA+GGG + chr1_1:54143172-54143191 None:intergenic 25.0%
! AAATGAAGAAAAATAGGAGT+AGG + chr1_1:54143173-54143192 None:intergenic 25.0%
TGGGTGAAATGAAGAAAAAT+AGG + chr1_1:54143179-54143198 None:intergenic 30.0%
AAAGATTGTTGAGAGAAAGT+GGG + chr1_1:54143198-54143217 None:intergenic 30.0%
TTATCAATCCAATCCTCTAA+TGG - chr1_1:54143552-54143571 Msa0029020:CDS 30.0%
GAAAGATTGTTGAGAGAAAG+TGG + chr1_1:54143199-54143218 None:intergenic 35.0%
CTTTCTCTCAACAATCTTTC+AGG - chr1_1:54143199-54143218 Msa0029020:CDS 35.0%
!! GGTTGATTTGAGCTTTGTAT+TGG + chr1_1:54143272-54143291 None:intergenic 35.0%
!! AAGTTGGTGCACATGAGAAT+AGG + chr1_1:54143614-54143633 None:intergenic 40.0%
! TTTTGTGGCTGGGAGAACTA+GGG + chr1_1:54143232-54143251 None:intergenic 45.0%
! TGTTGTAGCGCCATCTTTTG+TGG + chr1_1:54143247-54143266 None:intergenic 45.0%
GGTATGTGGAGGAAATGTAG+TGG + chr1_1:54143368-54143387 None:intergenic 45.0%
TGTTTCTCATCACCAAAGCC+TGG - chr1_1:54143447-54143466 Msa0029020:CDS 45.0%
GGTTGAAGCCATTAGAGGAT+TGG + chr1_1:54143563-54143582 None:intergenic 45.0%
AAACTGCGACATTTCGTCCT+GGG + chr1_1:54143584-54143603 None:intergenic 45.0%
! CTTTTGTGGCTGGGAGAACT+AGG + chr1_1:54143233-54143252 None:intergenic 50.0%
GTTCTCCCAGCCACAAAAGA+TGG - chr1_1:54143234-54143253 Msa0029020:CDS 50.0%
! TAGCGCCATCTTTTGTGGCT+GGG + chr1_1:54143242-54143261 None:intergenic 50.0%
! GTGGGACTAGTGGAGTTCTT+TGG + chr1_1:54143293-54143312 None:intergenic 50.0%
ATGACGTTGAGTGGGACTAG+TGG + chr1_1:54143303-54143322 None:intergenic 50.0%
GATCGACGATGACGTTGAGT+GGG + chr1_1:54143311-54143330 None:intergenic 50.0%
AGATCGACGATGACGTTGAG+TGG + chr1_1:54143312-54143331 None:intergenic 50.0%
CTCAACGTCATCGTCGATCT+CGG - chr1_1:54143312-54143331 Msa0029020:CDS 50.0%
CGCAATCAACCCAGAGGAAT+TGG + chr1_1:54143341-54143360 None:intergenic 50.0%
! GGTTTTCGCAATCAACCCAG+AGG + chr1_1:54143347-54143366 None:intergenic 50.0%
GAAACAATCACCACAGCCGT+CGG + chr1_1:54143433-54143452 None:intergenic 50.0%
! TTGTTGTTGCAGCCAGGCTT+TGG + chr1_1:54143462-54143481 None:intergenic 50.0%
!! TGTGGGTGATGGAATTGTCC+GGG + chr1_1:54143500-54143519 None:intergenic 50.0%
!! GTGTGGGTGATGGAATTGTC+CGG + chr1_1:54143501-54143520 None:intergenic 50.0%
ATCTTCAGCAAGTTCACCGC+TGG + chr1_1:54143529-54143548 None:intergenic 50.0%
TCCTCTAATGGCTTCAACCC+AGG - chr1_1:54143564-54143583 Msa0029020:CDS 50.0%
! TCCTGGGTTGAAGCCATTAG+AGG + chr1_1:54143568-54143587 None:intergenic 50.0%
GAAACTGCGACATTTCGTCC+TGG + chr1_1:54143585-54143604 None:intergenic 50.0%
! GCTGGGAGAACTAGGGCTTT+TGG + chr1_1:54143225-54143244 None:intergenic 55.0%
! GTAGCGCCATCTTTTGTGGC+TGG + chr1_1:54143243-54143262 None:intergenic 55.0%
AACGTCATCGTCGATCTCGG+TGG - chr1_1:54143315-54143334 Msa0029020:CDS 55.0%
ATCTCGGTGGCCAATTCCTC+TGG - chr1_1:54143328-54143347 Msa0029020:CDS 55.0%
TCTCGGTGGCCAATTCCTCT+GGG - chr1_1:54143329-54143348 Msa0029020:CDS 55.0%
!! GGAATTGGCGAGGGAGCATT+GGG + chr1_1:54143412-54143431 None:intergenic 55.0%
ATCACCACAGCCGTCGGAAT+TGG + chr1_1:54143427-54143446 None:intergenic 55.0%
!! CAGGTGTTGTTGTTGCAGCC+AGG + chr1_1:54143468-54143487 None:intergenic 55.0%
GGCTGCAACAACAACACCTG+TGG - chr1_1:54143468-54143487 Msa0029020:intron 55.0%
!! GTGGGTGATGGAATTGTCCG+GGG + chr1_1:54143499-54143518 None:intergenic 55.0%
TTCAGCAAGTTCACCGCTGG+TGG + chr1_1:54143526-54143545 None:intergenic 55.0%
! CGGAATTGGCGAGGGAGCAT+TGG + chr1_1:54143413-54143432 None:intergenic 60.0%
TGCTCCCTCGCCAATTCCGA+CGG - chr1_1:54143414-54143433 Msa0029020:CDS 60.0%
ACAGCCGTCGGAATTGGCGA+GGG + chr1_1:54143421-54143440 None:intergenic 60.0%
!! CAACACCTGTGGTGTCACCC+CGG - chr1_1:54143479-54143498 Msa0029020:intron 60.0%
ATTGTCCGGGGTGACACCAC+AGG + chr1_1:54143487-54143506 None:intergenic 60.0%
AGCAAGTTCACCGCTGGTGG+CGG + chr1_1:54143523-54143542 None:intergenic 60.0%
CCTCCACATACCGCCCTGCA+CGG - chr1_1:54143376-54143395 Msa0029020:CDS 65.0%
ACACCGTGCAGGGCGGTATG+TGG + chr1_1:54143382-54143401 None:intergenic 65.0%
CACAGCCGTCGGAATTGGCG+AGG + chr1_1:54143422-54143441 None:intergenic 65.0%
CTCGCCAATTCCGACGGCTG+TGG - chr1_1:54143420-54143439 Msa0029020:CDS 65.0%
ATCACCCACACCGCCACCAG+CGG - chr1_1:54143510-54143529 Msa0029020:intron 65.0%
!! TTCACCGCTGGTGGCGGTGT+GGG + chr1_1:54143517-54143536 None:intergenic 65.0%
CCGTGCAGGGCGGTATGTGG+AGG + chr1_1:54143379-54143398 None:intergenic 70.0%
CTGAGCGACACCGTGCAGGG+CGG + chr1_1:54143389-54143408 None:intergenic 70.0%
GGGCTGAGCGACACCGTGCA+GGG + chr1_1:54143392-54143411 None:intergenic 70.0%
TGGGCTGAGCGACACCGTGC+AGG + chr1_1:54143393-54143412 None:intergenic 70.0%
!! GCTGGTGGCGGTGTGGGTGA+TGG + chr1_1:54143511-54143530 None:intergenic 70.0%
!! GTTCACCGCTGGTGGCGGTG+TGG + chr1_1:54143518-54143537 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 54143138 54143642 54143138 ID=Msa0029020;Name=Msa0029020
chr1_1 mRNA 54143138 54143642 54143138 ID=Msa0029020-mRNA-1;Parent=Msa0029020;Name=Msa0029020-mRNA-1;_AED=0.42;_eAED=0.87;_QI=0|0|0|1|0|0|2|0|143
chr1_1 exon 54143547 54143642 54143547 ID=Msa0029020-mRNA-1:exon:16052;Parent=Msa0029020-mRNA-1
chr1_1 exon 54143138 54143473 54143138 ID=Msa0029020-mRNA-1:exon:16051;Parent=Msa0029020-mRNA-1
chr1_1 CDS 54143547 54143642 54143547 ID=Msa0029020-mRNA-1:cds;Parent=Msa0029020-mRNA-1
chr1_1 CDS 54143138 54143473 54143138 ID=Msa0029020-mRNA-1:cds;Parent=Msa0029020-mRNA-1
Gene Sequence

>Msa0029020

ATGGCTTCCAATTTTCAATACTTCATTACAACCCTACTCCTATTTTTCTTCATTTCACCCACTTTCTCTCAACAATCTTTCAGGCCAAAAGCCCTATCCCACTCAACGTCATCGTCGATCTCGGTGGCCAATTCCTCTGGGTTGATTGCGAAAACCACTACATTTCCTCCACATACCGCCCTGCACGGTGTCGCTCAGCCCAATGCTCCCTCGCCAATTCCGACGGCTGTGGTGATTGTTTCTCATCACCAAAGCCTGGCTGCAACAACAACACCTGTGGTGTCACCCCGGACAATTCCATCACCCACACCGCCACCAGCGGTGAACTTGCTGAAGATGTTTTATCAATCCAATCCTCTAATGGCTTCAACCCAGGACGAAATGTCGCAGTTTCACGCTTCCTATTCTCATGTGCACCAACTTTCTTACTAA

Protein sequence

>Msa0029020

MASNFQYFITTLLLFFFISPTFSQQSFRPKALSHSTSSSISVANSSGLIAKTTTFPPHTALHGVAQPNAPSPIPTAVVIVSHHQSLAATTTPVVSPRTIPSPTPPPAVNLLKMFYQSNPLMASTQDEMSQFHASYSHVHQLSY*