AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0081050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081050 MtrunA17_Chr1g0193391 97.049 610 14 1 1 610 1 606 0.0 1217
Msa0081050 MtrunA17_Chr7g0271221 81.424 576 104 3 1 575 1 574 0.0 970
Msa0081050 MtrunA17_Chr4g0069581 58.779 524 212 2 35 555 4 526 0.0 595
Msa0081050 MtrunA17_Chr3g0133341 47.461 571 263 8 11 564 10 560 0.0 541
Msa0081050 MtrunA17_Chr2g0284701 43.927 601 288 12 3 560 8 602 2.12e-164 483
Msa0081050 MtrunA17_Chr8g0379971 42.808 584 284 10 14 548 11 593 2.93e-147 439
Msa0081050 MtrunA17_Chr8g0379991 44.107 560 256 9 45 548 8 566 2.65e-141 422
Msa0081050 MtrunA17_Chr2g0276751 39.318 557 316 9 2 546 17 563 1.27e-140 421
Msa0081050 MtrunA17_Chr5g0415961 40.575 557 296 9 17 546 24 572 1.32e-140 421
Msa0081050 MtrunA17_Chr8g0362711 40.860 558 292 9 17 546 24 571 1.40e-136 410
Msa0081050 MtrunA17_Chr3g0142101 37.660 547 327 7 17 555 35 575 5.51e-127 386
Msa0081050 MtrunA17_Chr3g0142091 38.198 555 326 8 17 559 26 575 9.50e-125 380
Msa0081050 MtrunA17_Chr7g0241741 36.861 548 330 6 17 551 22 566 2.00e-121 371
Msa0081050 MtrunA17_Chr4g0005901 37.431 545 321 9 17 546 32 571 4.35e-120 368
Msa0081050 MtrunA17_Chr5g0422881 37.522 573 327 13 17 564 20 586 1.88e-116 358
Msa0081050 MtrunA17_Chr3g0104011 35.531 546 332 8 17 546 22 563 3.03e-115 355
Msa0081050 MtrunA17_Chr7g0214731 36.282 554 327 8 19 548 63 614 3.63e-115 357
Msa0081050 MtrunA17_Chr8g0375291 35.145 552 337 7 2 537 27 573 1.25e-114 354
Msa0081050 MtrunA17_Chr7g0240381 35.675 541 328 8 17 547 29 559 2.25e-114 353
Msa0081050 MtrunA17_Chr8g0375311 33.868 561 350 9 2 546 27 582 5.97e-113 350
Msa0081050 MtrunA17_Chr5g0399071 36.219 566 325 12 17 556 25 580 8.93e-112 347
Msa0081050 MtrunA17_Chr7g0261811 34.615 572 343 9 3 554 15 575 4.03e-111 345
Msa0081050 MtrunA17_Chr8g0375361 34.225 561 346 8 2 546 28 581 1.68e-110 343
Msa0081050 MtrunA17_Chr4g0008651 35.832 547 333 9 17 551 34 574 2.46e-110 343
Msa0081050 MtrunA17_Chr5g0399061 35.979 567 327 14 17 557 27 583 1.49e-109 341
Msa0081050 MtrunA17_Chr7g0261881 32.692 572 354 9 3 554 15 575 1.67e-108 338
Msa0081050 MtrunA17_Chr3g0112671 34.066 546 344 8 9 547 25 561 5.17e-108 337
Msa0081050 MtrunA17_Chr7g0261791 33.391 575 351 9 3 556 17 580 4.98e-106 332
Msa0081050 MtrunA17_Chr7g0261771 33.450 571 347 11 3 552 19 577 1.87e-105 331
Msa0081050 MtrunA17_Chr5g0439381 35.548 557 335 9 17 561 28 572 1.42e-104 328
Msa0081050 MtrunA17_Chr4g0056431 36.252 571 331 11 17 563 25 586 2.90e-104 327
Msa0081050 MtrunA17_Chr3g0114501 35.159 566 341 10 3 551 10 566 4.07e-104 327
Msa0081050 MtrunA17_Chr8g0375321 32.562 562 358 8 2 547 24 580 4.11e-103 324
Msa0081050 MtrunA17_Chr8g0375341 32.264 561 359 7 2 546 27 582 3.60e-102 322
Msa0081050 MtrunA17_Chr1g0213711 33.702 543 337 11 17 544 21 555 7.43e-102 321
Msa0081050 MtrunA17_Chr1g0166791 31.327 565 367 9 3 552 12 570 1.02e-101 321
Msa0081050 MtrunA17_Chr8g0375351 32.079 558 358 7 3 544 28 580 2.96e-101 319
Msa0081050 MtrunA17_Chr1g0187241 31.207 580 372 10 3 563 14 585 3.87e-101 319
Msa0081050 MtrunA17_Chr3g0112611 33.102 577 353 9 9 559 24 593 1.36e-99 315
Msa0081050 MtrunA17_Chr8g0375331 31.716 536 345 7 2 521 11 541 1.80e-99 314
Msa0081050 MtrunA17_Chr7g0261851 33.100 571 357 8 3 556 16 578 2.49e-99 315
Msa0081050 MtrunA17_Chr7g0255621 32.392 602 364 15 4 577 12 598 6.86e-98 311
Msa0081050 MtrunA17_Chr7g0217461 32.297 579 366 8 3 560 12 585 2.01e-97 309
Msa0081050 MtrunA17_Chr4g0063591 36.330 545 325 9 17 546 22 559 4.09e-97 308
Msa0081050 MtrunA17_Chr1g0166711 32.348 575 365 10 3 560 13 580 4.90e-97 308
Msa0081050 MtrunA17_Chr7g0255611 32.155 594 359 16 1 561 9 591 4.21e-96 306
Msa0081050 MtrunA17_Chr5g0400371 32.562 562 354 9 3 544 100 656 2.38e-95 306
Msa0081050 MtrunA17_Chr7g0214461 32.717 541 343 10 18 544 79 612 2.02e-94 303
Msa0081050 MtrunA17_Chr7g0255601 31.220 631 374 16 1 608 13 606 2.27e-93 299
Msa0081050 MtrunA17_Chr7g0261661 34.122 507 295 9 105 591 1 488 3.11e-93 296
Msa0081050 MtrunA17_Chr4g0054891 31.597 576 361 10 3 553 98 665 2.09e-92 298
Msa0081050 MtrunA17_Chr4g0059971 33.515 549 338 9 22 546 39 584 7.11e-92 295
Msa0081050 MtrunA17_Chr5g0415791 33.219 584 360 12 17 576 29 606 1.18e-91 295
Msa0081050 MtrunA17_Chr7g0218851 31.860 543 347 11 18 544 47 582 4.03e-91 293
Msa0081050 MtrunA17_Chr4g0011471 33.210 539 336 9 26 544 23 557 3.08e-89 287
Msa0081050 MtrunA17_Chr1g0166771 31.496 508 334 7 31 529 5 507 3.26e-89 286
Msa0081050 MtrunA17_Chr1g0166741 30.435 552 370 7 17 559 27 573 6.37e-89 287
Msa0081050 MtrunA17_Chr4g0059991 31.655 556 350 11 22 551 27 578 1.46e-87 284
Msa0081050 MtrunA17_Chr8g0353031 30.139 574 359 10 1 551 1 555 1.83e-85 278
Msa0081050 MtrunA17_Chr4g0014871 31.239 557 353 13 3 548 18 555 6.59e-83 270
Msa0081050 MtrunA17_Chr4g0059981 31.459 569 357 11 22 564 36 597 6.65e-83 271
Msa0081050 MtrunA17_Chr4g0014781 31.618 544 345 10 17 550 29 555 7.42e-83 270
Msa0081050 MtrunA17_Chr4g0014791 31.655 556 349 10 3 548 17 551 7.84e-83 270
Msa0081050 MtrunA17_Chr4g0014841 30.396 556 356 11 3 548 17 551 1.09e-81 267
Msa0081050 MtrunA17_Chr4g0060071 46.595 279 136 4 279 551 32 303 6.39e-81 257
Msa0081050 MtrunA17_Chr3g0077151 31.418 557 340 12 3 546 16 543 7.03e-81 265
Msa0081050 MtrunA17_Chr1g0166731 33.623 461 286 8 107 552 12 467 1.13e-80 262
Msa0081050 MtrunA17_Chr4g0014801 31.004 558 356 11 3 550 17 555 2.57e-80 263
Msa0081050 MtrunA17_Chr4g0060001 30.339 590 377 12 18 586 30 606 3.13e-79 262
Msa0081050 MtrunA17_Chr8g0390331 30.319 564 366 13 8 552 6 561 1.26e-78 259
Msa0081050 MtrunA17_Chr8g0362801 33.978 465 280 10 105 546 1 461 4.21e-78 255
Msa0081050 MtrunA17_Chr4g0011041 29.107 560 375 9 3 544 24 579 5.08e-78 258
Msa0081050 MtrunA17_Chr3g0082631 34.744 449 262 9 135 558 30 472 1.19e-77 254
Msa0081050 MtrunA17_Chr7g0234381 35.730 445 252 8 104 543 3 418 9.19e-77 250
Msa0081050 MtrunA17_Chr2g0331481 28.302 636 416 13 3 607 11 637 1.53e-76 256
Msa0081050 MtrunA17_Chr2g0331471 28.205 624 408 13 15 607 86 700 1.78e-76 257
Msa0081050 MtrunA17_Chr7g0234181 36.054 441 250 9 107 543 1 413 3.05e-76 248
Msa0081050 MtrunA17_Chr7g0240271 27.941 544 369 10 17 540 24 564 1.10e-75 252
Msa0081050 MtrunA17_Chr8g0362781 28.292 562 363 10 17 547 30 582 5.74e-75 250
Msa0081050 MtrunA17_Chr4g0003761 28.014 564 376 14 19 561 32 586 7.61e-75 250
Msa0081050 MtrunA17_Chr7g0234151 35.056 445 255 10 104 543 3 418 3.14e-74 243
Msa0081050 MtrunA17_Chr4g0014861 34.038 426 260 10 134 548 15 430 2.82e-73 241
Msa0081050 MtrunA17_Chr2g0331511 29.617 574 369 13 15 560 19 585 6.43e-73 246
Msa0081050 MtrunA17_Chr1g0157611 29.268 574 372 14 6 556 5 567 1.74e-71 240
Msa0081050 MtrunA17_Chr1g0157601 29.181 562 367 15 3 543 2 553 2.13e-71 240
Msa0081050 MtrunA17_Chr1g0147631 30.236 592 351 17 17 582 25 580 3.40e-71 240
Msa0081050 MtrunA17_Chr7g0240281 28.857 551 363 10 17 543 24 569 2.87e-69 235
Msa0081050 MtrunA17_Chr7g0261751 29.053 475 279 8 3 427 19 485 4.82e-69 233
Msa0081050 MtrunA17_Chr1g0177591 29.444 540 352 14 15 542 40 562 5.45e-68 231
Msa0081050 MtrunA17_Chr7g0235521 29.284 461 303 9 108 549 4 460 2.96e-66 224
Msa0081050 MtrunA17_Chr7g0240131 29.284 461 303 9 108 549 4 460 1.32e-65 222
Msa0081050 MtrunA17_Chr7g0240181 29.284 461 303 9 108 549 4 460 1.60e-65 222
Msa0081050 MtrunA17_Chr7g0240211 29.284 461 303 9 108 549 4 460 2.71e-65 221
Msa0081050 MtrunA17_Chr2g0331521 26.541 584 390 17 17 577 22 589 1.36e-62 217
Msa0081050 MtrunA17_Chr2g0331461 28.155 618 396 16 3 588 11 612 6.74e-62 216
Msa0081050 MtrunA17_Chr3g0142121 41.219 279 154 5 284 561 11 280 2.72e-60 202
Msa0081050 MtrunA17_Chr7g0240151 28.097 452 286 9 108 540 4 435 3.71e-58 202
Msa0081050 MtrunA17_Chr1g0157621 26.799 556 374 14 35 569 1 544 9.15e-58 203
Msa0081050 MtrunA17_Chr1g0177611 31.429 420 264 12 135 542 26 433 1.23e-57 200
Msa0081050 MtrunA17_Chr7g0261731 31.105 344 216 5 3 333 19 354 1.26e-56 195
Msa0081050 MtrunA17_Chr3g0112631 36.727 275 160 5 283 547 3 273 1.45e-56 192
Msa0081050 MtrunA17_Chr5g0415841 29.122 467 300 10 105 547 1 460 2.30e-56 197
Msa0081050 MtrunA17_Chr1g0177631 31.340 418 267 11 135 542 26 433 1.18e-55 195
Msa0081050 MtrunA17_Chr3g0142111 34.043 282 179 3 17 291 46 327 4.02e-53 185
Msa0081050 MtrunA17_Chr2g0326211 26.064 564 372 14 4 547 10 548 8.99e-53 189
Msa0081050 MtrunA17_Chr2g0325951 26.335 562 373 12 4 547 10 548 3.50e-52 187
Msa0081050 MtrunA17_Chr6g0451841 26.834 559 363 11 17 551 5 541 3.46e-51 185
Msa0081050 MtrunA17_Chr2g0325961 25.769 520 350 11 46 547 48 549 2.58e-49 180
Msa0081050 MtrunA17_Chr7g0261721 40.394 203 110 5 353 548 5 203 8.81e-37 137
Msa0081050 MtrunA17_Chr1g0147591 31.795 195 120 4 142 326 9 200 7.61e-26 105
Msa0081050 MtrunA17_Chr7g0261831 38.158 152 85 4 242 387 1 149 1.06e-25 105
Msa0081050 MtrunA17_Chr3g0112651 30.270 185 123 3 104 284 21 203 3.46e-24 100
Msa0081050 MtrunA17_Chr4g0060061 38.983 118 72 0 128 245 11 128 7.44e-23 94.7
Msa0081050 MtrunA17_Chr2g0326221 26.222 225 139 6 323 546 25 223 2.49e-15 75.5
Msa0081050 MtrunA17_Chr4g0060051 36.264 91 56 1 11 101 18 106 6.94e-15 71.6
Msa0081050 MtrunA17_Chr7g0234371 41.975 81 44 2 27 104 1 81 7.63e-12 61.6
Msa0081050 MtrunA17_Chr7g0234141 41.975 81 44 2 27 104 1 81 7.63e-12 61.6
Msa0081050 MtrunA17_Chr7g0234171 45.161 62 34 0 43 104 16 77 1.53e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081050 AT1G59740.1 41.873 566 307 11 5 553 23 583 6.39e-147 437
Msa0081050 AT1G69850.1 39.895 569 303 8 17 552 23 585 2.09e-141 423
Msa0081050 AT1G33440.1 41.516 554 311 7 19 563 38 587 8.28e-139 417
Msa0081050 AT1G27040.2 38.798 549 313 7 17 550 21 561 1.31e-134 405
Msa0081050 AT1G27040.1 38.798 549 313 7 17 550 25 565 1.55e-134 405
Msa0081050 AT1G27040.3 39.360 531 299 7 35 550 11 533 4.68e-131 395
Msa0081050 AT5G62730.1 37.636 550 311 7 17 549 54 588 6.51e-124 378
Msa0081050 AT5G62730.2 37.823 542 305 7 25 549 3 529 1.87e-122 373
Msa0081050 AT3G25280.1 39.205 528 291 10 19 543 21 521 2.48e-120 367
Msa0081050 AT3G54140.1 35.963 545 331 7 17 546 22 563 3.81e-120 368
Msa0081050 AT3G54140.2 35.963 545 331 7 17 546 22 563 3.81e-120 368
Msa0081050 AT5G01180.2 35.046 545 335 7 17 546 23 563 2.74e-117 360
Msa0081050 AT5G01180.3 35.046 545 335 7 17 546 23 563 2.74e-117 360
Msa0081050 AT5G01180.1 35.046 545 335 7 17 546 23 563 2.74e-117 360
Msa0081050 AT3G25260.1 36.364 528 300 10 19 543 21 515 1.15e-113 349
Msa0081050 AT1G62200.1 36.466 532 314 7 17 532 50 573 1.22e-113 352
Msa0081050 AT2G02040.1 34.727 550 340 7 17 550 39 585 6.11e-113 350
Msa0081050 AT5G13400.1 37.063 572 333 11 2 550 48 615 9.30e-113 350
Msa0081050 AT2G37900.1 35.436 539 333 7 17 547 36 567 1.81e-112 348
Msa0081050 AT1G62200.2 34.759 561 313 8 17 532 50 602 5.97e-108 338
Msa0081050 AT1G62200.3 34.759 561 313 8 17 532 64 616 1.09e-107 338
Msa0081050 AT2G37900.2 36.789 492 296 7 71 554 11 495 6.85e-106 329
Msa0081050 AT1G68570.1 37.234 564 326 12 18 558 22 580 2.61e-105 330
Msa0081050 AT2G26690.1 36.496 548 328 9 17 550 22 563 3.43e-105 330
Msa0081050 AT2G26690.2 37.248 545 317 11 29 559 4 537 1.53e-104 327
Msa0081050 AT3G53960.1 33.577 548 345 8 14 547 32 574 1.88e-104 328
Msa0081050 AT1G12110.1 34.146 574 349 9 17 570 25 589 3.53e-104 327
Msa0081050 AT2G26690.3 37.618 529 308 10 45 559 5 525 1.57e-103 324
Msa0081050 AT1G68570.2 37.226 548 319 11 31 558 8 550 2.14e-103 325
Msa0081050 AT2G40460.1 34.110 601 352 13 3 587 10 582 1.13e-101 320
Msa0081050 AT1G69870.1 34.266 572 344 10 17 560 49 616 1.38e-101 322
Msa0081050 AT3G21670.1 35.714 546 325 8 17 548 27 560 2.78e-101 320
Msa0081050 AT3G54450.1 32.624 564 341 9 17 559 10 555 3.32e-100 316
Msa0081050 AT4G21680.1 34.091 572 346 11 3 550 14 578 7.76e-98 311
Msa0081050 AT2G02020.1 31.657 537 325 5 17 545 40 542 1.29e-97 309
Msa0081050 AT1G32450.1 30.887 586 375 10 3 564 23 602 2.21e-94 302
Msa0081050 AT5G46040.1 30.329 577 375 10 1 557 6 575 6.54e-91 293
Msa0081050 AT1G18880.1 32.014 581 361 13 3 558 7 578 1.15e-88 286
Msa0081050 AT5G46050.1 30.909 550 356 9 20 552 28 570 4.14e-88 285
Msa0081050 AT1G72140.1 32.541 547 328 8 17 547 32 553 7.22e-87 281
Msa0081050 AT5G62680.1 31.228 570 354 13 22 561 46 607 5.18e-86 280
Msa0081050 AT5G19640.1 30.292 548 350 12 20 552 60 590 5.13e-85 278
Msa0081050 AT1G72130.1 30.869 541 341 9 17 550 23 537 8.56e-84 273
Msa0081050 AT1G27080.1 31.959 582 361 12 17 580 12 576 8.75e-83 271
Msa0081050 AT1G52190.1 29.932 588 377 13 17 576 19 599 1.70e-81 268
Msa0081050 AT5G28470.2 31.148 549 356 9 17 549 15 557 1.84e-80 264
Msa0081050 AT1G22570.1 31.429 525 329 10 17 524 28 538 3.33e-80 264
Msa0081050 AT3G47960.1 30.906 563 345 15 22 552 64 614 3.72e-78 260
Msa0081050 AT1G27080.2 32.051 546 337 11 48 575 1 530 4.44e-78 257
Msa0081050 AT1G22540.1 32.072 555 342 11 11 551 24 557 5.95e-78 258
Msa0081050 AT1G72125.1 31.801 544 341 12 21 549 31 559 1.81e-77 256
Msa0081050 AT5G28470.1 30.965 549 355 10 17 549 15 555 1.96e-77 256
Msa0081050 AT2G02020.2 27.933 537 324 6 17 545 40 521 3.07e-77 255
Msa0081050 AT1G69860.1 31.250 560 359 12 3 547 2 550 4.61e-77 255
Msa0081050 AT1G22550.1 30.672 551 348 10 17 549 28 562 5.43e-77 255
Msa0081050 AT1G22570.2 32.056 496 306 10 46 524 20 501 1.59e-76 253
Msa0081050 AT5G46050.2 31.866 477 300 10 105 564 1 469 5.30e-75 247
Msa0081050 AT1G72120.1 30.826 545 349 9 17 549 27 555 2.08e-74 248
Msa0081050 AT1G72130.2 34.043 423 252 8 135 550 17 419 4.12e-74 243
Msa0081050 AT1G72130.3 34.043 423 252 8 135 550 17 419 4.12e-74 243
Msa0081050 AT3G16180.1 28.924 567 362 13 5 542 9 563 5.20e-74 248
Msa0081050 AT3G45660.1 28.250 577 361 16 8 560 10 557 4.62e-67 229
Msa0081050 AT3G45660.3 28.250 577 361 16 8 560 10 557 4.62e-67 229
Msa0081050 AT3G45660.2 28.250 577 361 16 8 560 31 578 1.69e-66 228
Msa0081050 AT3G45650.1 28.172 536 342 13 44 560 47 558 1.42e-64 222
Msa0081050 AT3G45680.1 28.371 571 360 16 2 552 7 548 4.29e-64 221
Msa0081050 AT3G45720.1 29.191 507 326 13 60 552 59 546 6.29e-63 218
Msa0081050 AT3G45700.1 29.577 568 347 18 2 552 7 538 2.81e-62 216
Msa0081050 AT3G45710.1 28.772 570 361 17 2 552 7 550 1.14e-61 214
Msa0081050 AT3G45650.2 26.868 562 342 14 44 560 47 584 3.17e-59 208
Msa0081050 AT5G14940.1 27.599 558 367 13 17 552 5 547 5.00e-58 204
Msa0081050 AT3G45690.1 25.719 556 339 17 19 560 18 513 2.23e-56 199
Msa0081050 AT3G01350.1 25.487 565 380 11 17 552 5 557 5.64e-53 191
Msa0081050 AT5G11570.1 26.749 243 169 3 298 538 236 471 1.62e-24 107
Msa0081050 AT5G11570.1 26.126 222 153 5 20 234 14 231 2.18e-13 73.2
Msa0081050 AT2G38100.2 22.868 551 338 19 47 561 2 501 5.77e-24 106
Msa0081050 AT2G38100.1 22.868 551 338 19 47 561 18 517 1.62e-23 105
Msa0081050 AT2G38100.3 22.610 544 348 18 47 561 24 523 1.82e-23 105

Find 122 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAACCAATAAGCTTATTT+TGG 0.146048 1_2:-59560204 Msa0081050:CDS
GATAACCAAAATAAGCTTAT+TGG 0.163029 1_2:+59560199 None:intergenic
TTGTTTCTTCCATTGAATTA+TGG 0.165374 1_2:+59562658 None:intergenic
TCTTTCAAAAGTTTCTATTT+TGG 0.191150 1_2:-59562884 Msa0081050:five_prime_UTR
TTAGCTAGAGCTTACCATTT+TGG 0.198755 1_2:+59562782 None:intergenic
TGTTTCTTCCATTGAATTAT+GGG 0.210792 1_2:+59562659 None:intergenic
GGATCAGTTATTGGTGTTAC+TGG 0.239958 1_2:-59561763 Msa0081050:CDS
ATTTCTTTAACTTCCTCTAT+TGG 0.242727 1_2:-59559895 Msa0081050:CDS
ACACTGTTGGTTCTGTTCTT+TGG 0.247262 1_2:+59562983 None:intergenic
AACAAGAATGTTACCTATAT+TGG 0.263418 1_2:-59560545 Msa0081050:CDS
CTGTGAAAGAACCAAGTTTA+AGG 0.269342 1_2:+59560449 None:intergenic
AGGTGAAACACCTCTCTTAA+TGG 0.269533 1_2:-59559807 Msa0081050:CDS
TCAACTTTGGGATCAGTTAT+TGG 0.272879 1_2:-59561772 Msa0081050:CDS
CAACACTTTGACAAATTTCA+TGG 0.272897 1_2:-59562405 Msa0081050:CDS
CAGTATTGGCTTCACTAGTT+TGG 0.275066 1_2:+59559738 None:intergenic
ACAAAGAAAAGTTCATAAAT+TGG 0.278308 1_2:+59560373 None:intergenic
AGCTTAGTACTATACTTTAT+TGG 0.282321 1_2:-59562458 Msa0081050:CDS
ATCATTGTGTTTGTATGCTT+TGG 0.282815 1_2:-59561908 Msa0081050:CDS
ACTTTCTTGCTCTCCCTTGT+TGG 0.290210 1_2:-59562377 Msa0081050:CDS
TTATTAATACTGTTACTAAA+AGG 0.292645 1_2:-59560015 Msa0081050:CDS
TTTCTTTAACTTCCTCTATT+GGG 0.299260 1_2:-59559894 Msa0081050:CDS
CTTGATTTACCACATGCTTT+AGG 0.299383 1_2:+59561967 None:intergenic
AGAGCTTCCATGTTCATCTT+TGG 0.307984 1_2:-59562623 Msa0081050:intron
ATGGAGAATACAAAGGAATT+AGG 0.323607 1_2:-59559827 Msa0081050:CDS
ATTGGTACTATTGTTGGTTC+TGG 0.324362 1_2:-59559528 Msa0081050:three_prime_UTR
TTGGTGTTACTGGTATTGTT+TGG 0.332141 1_2:-59561753 Msa0081050:CDS
ACAATTGTACTTGCCAATAT+AGG 0.334608 1_2:+59560532 None:intergenic
ACCTGAATAATCCTCAAAAT+AGG 0.335933 1_2:+59561610 None:intergenic
TCATTGTGTTTGTATGCTTT+GGG 0.336923 1_2:-59561907 Msa0081050:CDS
AAGAAAATTAGTGGGATAAC+AGG 0.341586 1_2:+59560409 None:intergenic
GCAGACATGTTCACTCTTGT+TGG 0.342817 1_2:-59560151 Msa0081050:CDS
TGAAGTTGTTCATTAGATTC+TGG 0.346882 1_2:+59561467 None:intergenic
AGAGGTTCATTAACAGCATT+TGG 0.351216 1_2:-59561871 Msa0081050:CDS
TTTCCATACTCTCTCCAAAA+TGG 0.361256 1_2:-59562796 Msa0081050:intron
ATATAGGTAACATTCTTGTT+AGG 0.364001 1_2:+59560548 None:intergenic
TGGTGTTACTGGTATTGTTT+GGG 0.373138 1_2:-59561752 Msa0081050:CDS
TGCTAACCAATTTGATGAAA+AGG 0.379188 1_2:-59561848 Msa0081050:CDS
ATAACAGTAGCTTCTTCAAT+TGG 0.385104 1_2:-59561691 Msa0081050:CDS
AAAATCACTCACCACCCTTC+AGG 0.386975 1_2:-59560337 Msa0081050:CDS
TGTAACCAACCTTGTTTACT+TGG 0.393968 1_2:+59559986 None:intergenic
TAAACATTGGATGTGCAAAT+AGG 0.396118 1_2:-59559599 Msa0081050:three_prime_UTR
TGAAGTAGAAAGTGATTTCA+TGG 0.400504 1_2:+59560093 None:intergenic
AGAGGAAGAATAACCTCATT+TGG 0.402708 1_2:+59561399 None:intergenic
TGTTTGTATGCTTTGGGAAT+GGG 0.403406 1_2:-59561901 Msa0081050:CDS
GCCATTTCAATGACAATAGC+TGG 0.413834 1_2:-59560271 Msa0081050:CDS
ACCAGCTATTGTCATTGAAA+TGG 0.417173 1_2:+59560270 None:intergenic
AAGACATTGATATGATGTTA+AGG 0.427484 1_2:-59559648 Msa0081050:three_prime_UTR
ACTTCCTCTATTGGGCCTCT+TGG 0.427686 1_2:-59559886 Msa0081050:CDS
GTGTTTGTATGCTTTGGGAA+TGG 0.428271 1_2:-59561902 Msa0081050:CDS
AGTAAACAAGGTTGGTTACA+AGG 0.439747 1_2:-59559983 Msa0081050:CDS
GTTAAGGAAAACATAGTAGT+AGG 0.451430 1_2:-59559632 Msa0081050:three_prime_UTR
TTGGGAATGGGTGGAGTAAG+AGG 0.455587 1_2:-59561889 Msa0081050:CDS
GAAAGTGATTTCATGGTTGA+AGG 0.467352 1_2:+59560100 None:intergenic
TAATTGATTGGTACTATTGT+TGG 0.467510 1_2:-59559534 Msa0081050:three_prime_UTR
CAACAGATTCATCACATGTC+TGG 0.475260 1_2:-59562330 Msa0081050:CDS
ACATAGTAGTAGGTTGTGTA+TGG 0.485213 1_2:-59559622 Msa0081050:three_prime_UTR
TGCGTGCTTGTATAATTGAT+TGG 0.487662 1_2:-59559546 Msa0081050:three_prime_UTR
AATTCAATGGAAGAAACAAA+AGG 0.488343 1_2:-59562654 Msa0081050:CDS
AATGTGCATAAGAAAATTAG+TGG 0.493180 1_2:+59560400 None:intergenic
GATCACAGTTCAAGCTGCAT+TGG 0.495046 1_2:-59562001 Msa0081050:CDS
AACACTTTGACAAATTTCAT+GGG 0.510777 1_2:-59562404 Msa0081050:CDS
CAAACCTTCCATGGTTGTTG+AGG 0.513690 1_2:+59560601 None:intergenic
AACTCAAACCCATAATTCAA+TGG 0.515700 1_2:-59562667 Msa0081050:CDS
TTGTATGCTTTGGGAATGGG+TGG 0.519214 1_2:-59561898 Msa0081050:CDS
TTTGTCAGCATTGGATAACA+TGG 0.520721 1_2:-59562501 Msa0081050:CDS
TACTATTGTTGGTTCTGGCA+CGG 0.522892 1_2:-59559523 Msa0081050:three_prime_UTR
GAGGTAAGTATCTGAGATGA+AGG 0.523013 1_2:+59562352 None:intergenic
AGAAGAGGGTCCCTCAGTAT+TGG 0.526736 1_2:+59559724 None:intergenic
TAAATACTAGAATACTAGAA+TGG 0.529818 1_2:-59563016 Msa0081050:five_prime_UTR
TCTTCTGATGAAACAGATGA+TGG 0.532640 1_2:-59559704 Msa0081050:CDS
TGTTCATTAGATTCTGGCAG+TGG 0.534338 1_2:+59561473 None:intergenic
ATTCAATGGAAGAAACAAAA+GGG 0.537445 1_2:-59562653 Msa0081050:CDS
ACACCTCTCTTAATGGTTGG+TGG 0.538286 1_2:-59559800 Msa0081050:CDS
GTCACTGTGCAAACCTTCCA+TGG 0.538545 1_2:+59560592 None:intergenic
AGATAGTAACAATTCAATGA+TGG 0.541244 1_2:-59559846 Msa0081050:CDS
ATCTGTTTCATCAGAAGAAG+AGG 0.542967 1_2:+59559709 None:intergenic
CTCTGTGTGTGCAGAGTCAA+AGG 0.543882 1_2:-59559491 Msa0081050:three_prime_UTR
AATTAGTGGGATAACAGGAA+TGG 0.550662 1_2:+59560414 None:intergenic
CATGAAATTTGTCAAAGTGT+TGG 0.551927 1_2:+59562406 None:intergenic
GTTACACAGCTTCAAAGAGT+TGG 0.552152 1_2:-59560313 Msa0081050:CDS
TGTACCAAGAGGCCCAATAG+AGG 0.556413 1_2:+59559882 None:intergenic
AATGCAATGAACCTTAAACT+TGG 0.561968 1_2:-59560460 Msa0081050:CDS
TTGAGATTGAAATGAGAAGA+AGG 0.571870 1_2:+59562848 None:intergenic
TGAAGCTGTGTAACACCTGA+AGG 0.572853 1_2:+59560322 None:intergenic
AAAACATGATGATAAAGCTA+AGG 0.573309 1_2:-59559774 Msa0081050:CDS
GAAGTTAAAAGAAGAGACCA+AGG 0.573829 1_2:-59560241 Msa0081050:CDS
TGAGATGAAGGCACCAACAA+GGG 0.577394 1_2:+59562364 None:intergenic
GACGGTGAGCACAGAAACCA+AGG 0.581377 1_2:-59563075 Msa0081050:five_prime_UTR
TGAACCTCAACAACCATGGA+AGG 0.582478 1_2:-59560605 Msa0081050:CDS
CAGCTTCAAAGAGTTGGTGT+TGG 0.586012 1_2:-59560307 Msa0081050:CDS
CAAACATTCTCAGTACAACA+AGG 0.586833 1_2:-59560484 Msa0081050:CDS
TTGCACACACTAACCAAATG+AGG 0.588659 1_2:-59561412 Msa0081050:intron
ATAACAGGAATGGATGATGC+AGG 0.589149 1_2:+59560424 None:intergenic
CTGAGATGAAGGCACCAACA+AGG 0.591772 1_2:+59562363 None:intergenic
CTTCCACCAACCATTAAGAG+AGG 0.596589 1_2:+59559797 None:intergenic
AAATCAAGTTGTGTGGAAGG+AGG 0.597719 1_2:-59561952 Msa0081050:CDS
TTGTCAGCATTGGATAACAT+GGG 0.598817 1_2:-59562500 Msa0081050:CDS
CAAGAAGTAACCAAGTGACA+TGG 0.600735 1_2:+59560057 None:intergenic
CTCAAAATAGGACTTTCACC+AGG 0.610110 1_2:+59561622 None:intergenic
GAAACACCTCTCTTAATGGT+TGG 0.612482 1_2:-59559803 Msa0081050:CDS
ATTCTGAACCTCAACAACCA+TGG 0.613634 1_2:-59560609 Msa0081050:CDS
TTGGATGTGCAAATAGGAAG+TGG 0.626202 1_2:-59559593 Msa0081050:three_prime_UTR
TTCAATGATGGAGAATACAA+AGG 0.626874 1_2:-59559834 Msa0081050:CDS
TCACACCAAGTAAACAAGGT+TGG 0.627331 1_2:-59559991 Msa0081050:CDS
ATGTGCATAAGAAAATTAGT+GGG 0.636944 1_2:+59560401 None:intergenic
ACTAGTAAAGCATGTTAAGA+CGG 0.638731 1_2:-59563093 Msa0081050:five_prime_UTR
AGGATCACACCAAGTAAACA+AGG 0.639605 1_2:-59559995 Msa0081050:CDS
TGATTTGTACTTGTACCAAG+AGG 0.641427 1_2:+59559871 None:intergenic
TCTGTTTCATCAGAAGAAGA+GGG 0.647970 1_2:+59559710 None:intergenic
AATTTACATCCTAAAGCATG+TGG 0.649108 1_2:-59561976 Msa0081050:CDS
GGTAAATCAAGTTGTGTGGA+AGG 0.653776 1_2:-59561955 Msa0081050:CDS
CAATGGAAGAAACAAAAGGG+TGG 0.662396 1_2:-59562650 Msa0081050:CDS
ACTAGTGAAGCCAATACTGA+GGG 0.666695 1_2:-59559734 Msa0081050:CDS
AGGTTGTGTATGGTAAACAT+TGG 0.668701 1_2:-59559612 Msa0081050:three_prime_UTR
AACTAGTGAAGCCAATACTG+AGG 0.674814 1_2:-59559735 Msa0081050:CDS
GAAGCTGTGTAACACCTGAA+GGG 0.679710 1_2:+59560323 None:intergenic
GACATGTGATGAATCTGTTG+AGG 0.680872 1_2:+59562333 None:intergenic
AAACATTCTCAGTACAACAA+GGG 0.685525 1_2:-59560483 Msa0081050:CDS
GTACTTACCAAAGATGAACA+TGG 0.689840 1_2:+59562616 None:intergenic
ATGTGGTAAATCAAGTTGTG+TGG 0.691318 1_2:-59561959 Msa0081050:CDS
ACGGTGAGCACAGAAACCAA+GGG 0.709215 1_2:-59563074 Msa0081050:five_prime_UTR
GCTGTGTAACACCTGAAGGG+TGG 0.734299 1_2:+59560326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTCTTTATTTTATGTAAT+TGG + chr1_2:59560263-59560282 None:intergenic 15.0%
!! AGAAAGAAAAAAAAAACATT+TGG - chr1_2:59560277-59560296 Msa0081050:CDS 15.0%
!! TTATCACAAAATGTTAATTA+AGG + chr1_2:59561117-59561136 None:intergenic 15.0%
!!! GATATTTATTTATTTTGAGA+GGG - chr1_2:59561632-59561651 Msa0081050:CDS 15.0%
!! TTATTAATACTGTTACTAAA+AGG - chr1_2:59562461-59562480 Msa0081050:CDS 15.0%
!! TAAATACTAGAATACTAGAA+TGG - chr1_2:59559460-59559479 Msa0081050:three_prime_UTR 20.0%
!!! TCTTTCAAAAGTTTCTATTT+TGG - chr1_2:59559592-59559611 Msa0081050:three_prime_UTR 20.0%
!!! CTTTCAAAAGTTTCTATTTT+GGG - chr1_2:59559593-59559612 Msa0081050:three_prime_UTR 20.0%
!!! AGTTTGAAATATGTTACTAT+AGG - chr1_2:59559765-59559784 Msa0081050:CDS 20.0%
!!! GTTTGAAATATGTTACTATA+GGG - chr1_2:59559766-59559785 Msa0081050:CDS 20.0%
!!! AAAAACAAAGAGTGTTAATT+TGG + chr1_2:59559896-59559915 None:intergenic 20.0%
!!! TGTAACAAAACATATTGATT+TGG - chr1_2:59559925-59559944 Msa0081050:CDS 20.0%
!!! TATTTTATGTAATTGGAACA+TGG + chr1_2:59560256-59560275 None:intergenic 20.0%
!!! TATCACTTTTTCTTATGTTT+TGG + chr1_2:59561360-59561379 None:intergenic 20.0%
!!! CAAGTACCAATTATTTATTT+TGG - chr1_2:59561499-59561518 Msa0081050:CDS 20.0%
!! ATGGTTCCAAAATAAATAAT+TGG + chr1_2:59561508-59561527 None:intergenic 20.0%
!!! GGATATTTATTTATTTTGAG+AGG - chr1_2:59561631-59561650 Msa0081050:CDS 20.0%
!! ACAAAGAAAAGTTCATAAAT+TGG + chr1_2:59562106-59562125 None:intergenic 20.0%
!!! TGAGTGATTTTTCTAATAAA+TGG + chr1_2:59562130-59562149 None:intergenic 20.0%
!!! TCTTTTCAATATGCTATATT+TGG - chr1_2:59562298-59562317 Msa0081050:intron 20.0%
!!! ACAATTTGAACTTGTTTTAT+TGG - chr1_2:59562533-59562552 Msa0081050:intron 20.0%
!! TTTTTAATGTCTTACACTGT+TGG + chr1_2:59559509-59559528 None:intergenic 25.0%
! TTAAAAATAAGAACCTCTTG+TGG - chr1_2:59559522-59559541 Msa0081050:three_prime_UTR 25.0%
! TGTTTCTTCCATTGAATTAT+GGG + chr1_2:59559820-59559839 None:intergenic 25.0%
!! TTGTTTCTTCCATTGAATTA+TGG + chr1_2:59559821-59559840 None:intergenic 25.0%
! AATTCAATGGAAGAAACAAA+AGG - chr1_2:59559822-59559841 Msa0081050:CDS 25.0%
! ATTCAATGGAAGAAACAAAA+GGG - chr1_2:59559823-59559842 Msa0081050:CDS 25.0%
! AGCTTAGTACTATACTTTAT+TGG - chr1_2:59560018-59560037 Msa0081050:CDS 25.0%
! AACACTTTGACAAATTTCAT+GGG - chr1_2:59560072-59560091 Msa0081050:CDS 25.0%
!! AAAAAACATTTGGTTTAGAG+AGG - chr1_2:59560287-59560306 Msa0081050:CDS 25.0%
!! TTTTGTCTGGTACAAAATTA+CGG - chr1_2:59560351-59560370 Msa0081050:CDS 25.0%
! AATACTTGTATGTATTGTGT+AGG - chr1_2:59560442-59560461 Msa0081050:CDS 25.0%
!! CTTTTACTTAGTTCAACTTT+GGG - chr1_2:59560692-59560711 Msa0081050:intron 25.0%
!!! TAACCATTATTGTTTTATGC+AGG - chr1_2:59560948-59560967 Msa0081050:intron 25.0%
! TAACCTGCATAAAACAATAA+TGG + chr1_2:59560954-59560973 None:intergenic 25.0%
!!! AACATTTTGTGATAATGTGT+TGG - chr1_2:59561122-59561141 Msa0081050:intron 25.0%
! ATTAAGTAATGCCAAGAATT+TGG - chr1_2:59561242-59561261 Msa0081050:intron 25.0%
! TTAAGTAATGCCAAGAATTT+GGG - chr1_2:59561243-59561262 Msa0081050:intron 25.0%
! AAATACCACAAAGAAAAAGA+GGG + chr1_2:59561434-59561453 None:intergenic 25.0%
! CTATGTTATCTACAAAGTAA+AGG + chr1_2:59561468-59561487 None:intergenic 25.0%
!!! TTTATTTATTTTGAGAGGGT+AGG - chr1_2:59561636-59561655 Msa0081050:CDS 25.0%
!!! TTATTTATTTTGAGAGGGTA+GGG - chr1_2:59561637-59561656 Msa0081050:CDS 25.0%
! TTGTGTAAAGGGAAATAAAT+GGG + chr1_2:59561764-59561783 None:intergenic 25.0%
! TTTGTGTAAAGGGAAATAAA+TGG + chr1_2:59561765-59561784 None:intergenic 25.0%
! CATGCATTTATTTGTGTAAA+GGG + chr1_2:59561775-59561794 None:intergenic 25.0%
! GCATTTATACTCAAATGTTA+TGG - chr1_2:59561794-59561813 Msa0081050:CDS 25.0%
! ATATAGGTAACATTCTTGTT+AGG + chr1_2:59561931-59561950 None:intergenic 25.0%
! AACAAGAATGTTACCTATAT+TGG - chr1_2:59561931-59561950 Msa0081050:CDS 25.0%
! ATGTGCATAAGAAAATTAGT+GGG + chr1_2:59562078-59562097 None:intergenic 25.0%
! AATGTGCATAAGAAAATTAG+TGG + chr1_2:59562079-59562098 None:intergenic 25.0%
!!! ATAAGCTTATTGGTTTTGAA+GGG + chr1_2:59562270-59562289 None:intergenic 25.0%
!!! AATAAGCTTATTGGTTTTGA+AGG + chr1_2:59562271-59562290 None:intergenic 25.0%
!! CAAAACCAATAAGCTTATTT+TGG - chr1_2:59562272-59562291 Msa0081050:intron 25.0%
! GATAACCAAAATAAGCTTAT+TGG + chr1_2:59562280-59562299 None:intergenic 25.0%
!! ATTTCTTTAACTTCCTCTAT+TGG - chr1_2:59562581-59562600 Msa0081050:intron 25.0%
!! TTTCTTTAACTTCCTCTATT+GGG - chr1_2:59562582-59562601 Msa0081050:intron 25.0%
! AGATAGTAACAATTCAATGA+TGG - chr1_2:59562630-59562649 Msa0081050:CDS 25.0%
! AAAACATGATGATAAAGCTA+AGG - chr1_2:59562702-59562721 Msa0081050:intron 25.0%
! AAGACATTGATATGATGTTA+AGG - chr1_2:59562828-59562847 Msa0081050:five_prime_UTR 25.0%
! TAATTGATTGGTACTATTGT+TGG - chr1_2:59562942-59562961 Msa0081050:five_prime_UTR 25.0%
ACTAGTAAAGCATGTTAAGA+CGG - chr1_2:59559383-59559402 Msa0081050:three_prime_UTR 30.0%
AAAAAAAGGTAGAAAGAGTG+AGG - chr1_2:59559425-59559444 Msa0081050:three_prime_UTR 30.0%
TGATCATAAAAATCCACAAG+AGG + chr1_2:59559538-59559557 None:intergenic 30.0%
TTGAGATTGAAATGAGAAGA+AGG + chr1_2:59559631-59559650 None:intergenic 30.0%
CAAACTGTTACAAAAGAGAA+GGG + chr1_2:59559751-59559770 None:intergenic 30.0%
TCAAACTGTTACAAAAGAGA+AGG + chr1_2:59559752-59559771 None:intergenic 30.0%
AACTCAAACCCATAATTCAA+TGG - chr1_2:59559809-59559828 Msa0081050:CDS 30.0%
CATGAAATTTGTCAAAGTGT+TGG + chr1_2:59560073-59560092 None:intergenic 30.0%
CAACACTTTGACAAATTTCA+TGG - chr1_2:59560071-59560090 Msa0081050:CDS 30.0%
TTATGAGTTTGACAGAATCA+CGG - chr1_2:59560320-59560339 Msa0081050:CDS 30.0%
AATTTACATCCTAAAGCATG+TGG - chr1_2:59560500-59560519 Msa0081050:CDS 30.0%
ATCATTGTGTTTGTATGCTT+TGG - chr1_2:59560568-59560587 Msa0081050:CDS 30.0%
TCATTGTGTTTGTATGCTTT+GGG - chr1_2:59560569-59560588 Msa0081050:CDS 30.0%
TGCTAACCAATTTGATGAAA+AGG - chr1_2:59560628-59560647 Msa0081050:CDS 30.0%
! TTGGATCCTTTTCATCAAAT+TGG + chr1_2:59560637-59560656 None:intergenic 30.0%
!!! CACTTGCAACTTTTTTCAAT+TGG - chr1_2:59560669-59560688 Msa0081050:intron 30.0%
! GCTTTTACTTAGTTCAACTT+TGG - chr1_2:59560691-59560710 Msa0081050:intron 30.0%
ATAACAGTAGCTTCTTCAAT+TGG - chr1_2:59560785-59560804 Msa0081050:intron 30.0%
!! TCCTATTTTGAGGATTATTC+AGG - chr1_2:59560865-59560884 Msa0081050:intron 30.0%
ACCTGAATAATCCTCAAAAT+AGG + chr1_2:59560869-59560888 None:intergenic 30.0%
! TTTTATGCAGGTTATTGTTG+TGG - chr1_2:59560960-59560979 Msa0081050:intron 30.0%
TGAAGTTGTTCATTAGATTC+TGG + chr1_2:59561012-59561031 None:intergenic 30.0%
ATAATGTGTTGGACTAACTT+AGG - chr1_2:59561133-59561152 Msa0081050:intron 30.0%
AAAGAATCTTAACAATGTGC+TGG + chr1_2:59561205-59561224 None:intergenic 30.0%
GTTAACTAAAAAGCAAAGAG+AGG + chr1_2:59561336-59561355 None:intergenic 30.0%
! TTTTTCCCTCTTTTTCTTTG+TGG - chr1_2:59561426-59561445 Msa0081050:CDS 30.0%
CAAATACCACAAAGAAAAAG+AGG + chr1_2:59561435-59561454 None:intergenic 30.0%
! CTCTTTTTCTTTGTGGTATT+TGG - chr1_2:59561433-59561452 Msa0081050:CDS 30.0%
!!! TATTTATTTTGAGAGGGTAG+GGG - chr1_2:59561638-59561657 Msa0081050:CDS 30.0%
AATTGCTAAGGTGAAATTGA+AGG - chr1_2:59561667-59561686 Msa0081050:CDS 30.0%
GATGACAAAGATATTGTTTG+TGG - chr1_2:59561714-59561733 Msa0081050:CDS 30.0%
GCATGCATTTATTTGTGTAA+AGG + chr1_2:59561776-59561795 None:intergenic 30.0%
! ACTCAAATGTTATGGTTTGT+TGG - chr1_2:59561802-59561821 Msa0081050:CDS 30.0%
ACAATTGTACTTGCCAATAT+AGG + chr1_2:59561947-59561966 None:intergenic 30.0%
AAACATTCTCAGTACAACAA+GGG - chr1_2:59561993-59562012 Msa0081050:CDS 30.0%
AATGCAATGAACCTTAAACT+TGG - chr1_2:59562016-59562035 Msa0081050:intron 30.0%
AAGAAAATTAGTGGGATAAC+AGG + chr1_2:59562070-59562089 None:intergenic 30.0%
TGAAGTAGAAAGTGATTTCA+TGG + chr1_2:59562386-59562405 None:intergenic 30.0%
! TTCAATGATGGAGAATACAA+AGG - chr1_2:59562642-59562661 Msa0081050:CDS 30.0%
ATGGAGAATACAAAGGAATT+AGG - chr1_2:59562649-59562668 Msa0081050:CDS 30.0%
GTTAAGGAAAACATAGTAGT+AGG - chr1_2:59562844-59562863 Msa0081050:five_prime_UTR 30.0%
TAAACATTGGATGTGCAAAT+AGG - chr1_2:59562877-59562896 Msa0081050:five_prime_UTR 30.0%
ACAGAAACCAAGGGAAAAAA+AGG - chr1_2:59559411-59559430 Msa0081050:three_prime_UTR 35.0%
!!! CTTTCTACCTTTTTTTCCCT+TGG + chr1_2:59559421-59559440 None:intergenic 35.0%
TTTCCATACTCTCTCCAAAA+TGG - chr1_2:59559680-59559699 Msa0081050:CDS 35.0%
!! TTACCATTTTGGAGAGAGTA+TGG + chr1_2:59559686-59559705 None:intergenic 35.0%
! TTAGCTAGAGCTTACCATTT+TGG + chr1_2:59559697-59559716 None:intergenic 35.0%
GTACTTACCAAAGATGAACA+TGG + chr1_2:59559863-59559882 None:intergenic 35.0%
! TTTGTCAGCATTGGATAACA+TGG - chr1_2:59559975-59559994 Msa0081050:CDS 35.0%
! TTGTCAGCATTGGATAACAT+GGG - chr1_2:59559976-59559995 Msa0081050:CDS 35.0%
!!! TTCATCACATGTCTGGTTTT+TGG - chr1_2:59560153-59560172 Msa0081050:CDS 35.0%
TGTAATTGGAACATGGCATT+GGG + chr1_2:59560249-59560268 None:intergenic 35.0%
ATGTAATTGGAACATGGCAT+TGG + chr1_2:59560250-59560269 None:intergenic 35.0%
TGTATGTATTGTGTAGGCTT+TGG - chr1_2:59560448-59560467 Msa0081050:CDS 35.0%
CTTGATTTACCACATGCTTT+AGG + chr1_2:59560512-59560531 None:intergenic 35.0%
! ATGTGGTAAATCAAGTTGTG+TGG - chr1_2:59560517-59560536 Msa0081050:CDS 35.0%
!! TGTTTGTATGCTTTGGGAAT+GGG - chr1_2:59560575-59560594 Msa0081050:CDS 35.0%
AGAGGTTCATTAACAGCATT+TGG - chr1_2:59560605-59560624 Msa0081050:CDS 35.0%
!! TCAACTTTGGGATCAGTTAT+TGG - chr1_2:59560704-59560723 Msa0081050:intron 35.0%
!! TTGGTGTTACTGGTATTGTT+TGG - chr1_2:59560723-59560742 Msa0081050:intron 35.0%
!! TGGTGTTACTGGTATTGTTT+GGG - chr1_2:59560724-59560743 Msa0081050:intron 35.0%
TACACAAAAAGCTTGGCATT+GGG - chr1_2:59560751-59560770 Msa0081050:intron 35.0%
!!! GGTTTTGTAACACTTGCTAT+TGG - chr1_2:59560806-59560825 Msa0081050:intron 35.0%
!! GGAGTTTTGATACGGTAAAA+AGG + chr1_2:59560836-59560855 None:intergenic 35.0%
! TTTTACCGTATCAAAACTCC+TGG - chr1_2:59560836-59560855 Msa0081050:intron 35.0%
!! TTTCACCAGGAGTTTTGATA+CGG + chr1_2:59560844-59560863 None:intergenic 35.0%
AGAGGAAGAATAACCTCATT+TGG + chr1_2:59561080-59561099 None:intergenic 35.0%
AAGGCATGATGAATATGTAG+AGG + chr1_2:59561098-59561117 None:intergenic 35.0%
AGACTTACAGAGAATGATCA+TGG - chr1_2:59561301-59561320 Msa0081050:intron 35.0%
!! TTTTTCAGTGAGTTTGTGTC+AGG + chr1_2:59561394-59561413 None:intergenic 35.0%
CGATTAGGTCATACTTTCTA+TGG + chr1_2:59561527-59561546 None:intergenic 35.0%
! TAGAAAGTATGACCTAATCG+CGG - chr1_2:59561527-59561546 Msa0081050:intron 35.0%
CTGATTTCGTTGTGAAATCA+GGG + chr1_2:59561560-59561579 None:intergenic 35.0%
CTGATTTCACAACGAAATCA+GGG - chr1_2:59561559-59561578 Msa0081050:intron 35.0%
TGATTCAAAACACACGCTAA+GGG - chr1_2:59561610-59561629 Msa0081050:intron 35.0%
!! ATGGTTTGTTGGATTGAATC+AGG - chr1_2:59561813-59561832 Msa0081050:CDS 35.0%
CAAACATTCTCAGTACAACA+AGG - chr1_2:59561992-59562011 Msa0081050:CDS 35.0%
CTGTGAAAGAACCAAGTTTA+AGG + chr1_2:59562030-59562049 None:intergenic 35.0%
AATTAGTGGGATAACAGGAA+TGG + chr1_2:59562065-59562084 None:intergenic 35.0%
ACCAGCTATTGTCATTGAAA+TGG + chr1_2:59562209-59562228 None:intergenic 35.0%
GAAGTTAAAAGAAGAGACCA+AGG - chr1_2:59562235-59562254 Msa0081050:intron 35.0%
GAAAGTGATTTCATGGTTGA+AGG + chr1_2:59562379-59562398 None:intergenic 35.0%
! TGTAACCAACCTTGTTTACT+TGG + chr1_2:59562493-59562512 None:intergenic 35.0%
! AGTAAACAAGGTTGGTTACA+AGG - chr1_2:59562493-59562512 Msa0081050:CDS 35.0%
! TGATTTGTACTTGTACCAAG+AGG + chr1_2:59562608-59562627 None:intergenic 35.0%
TCTGTTTCATCAGAAGAAGA+GGG + chr1_2:59562769-59562788 None:intergenic 35.0%
ATCTGTTTCATCAGAAGAAG+AGG + chr1_2:59562770-59562789 None:intergenic 35.0%
TCTTCTGATGAAACAGATGA+TGG - chr1_2:59562772-59562791 Msa0081050:intron 35.0%
ACATAGTAGTAGGTTGTGTA+TGG - chr1_2:59562854-59562873 Msa0081050:five_prime_UTR 35.0%
AGGTTGTGTATGGTAAACAT+TGG - chr1_2:59562864-59562883 Msa0081050:five_prime_UTR 35.0%
TGCGTGCTTGTATAATTGAT+TGG - chr1_2:59562930-59562949 Msa0081050:five_prime_UTR 35.0%
!! ATTGGTACTATTGTTGGTTC+TGG - chr1_2:59562948-59562967 Msa0081050:five_prime_UTR 35.0%
!! ACACTGTTGGTTCTGTTCTT+TGG + chr1_2:59559496-59559515 None:intergenic 40.0%
CAATGGAAGAAACAAAAGGG+TGG - chr1_2:59559826-59559845 Msa0081050:CDS 40.0%
AGAGCTTCCATGTTCATCTT+TGG - chr1_2:59559853-59559872 Msa0081050:CDS 40.0%
!! TGATGCAGTTTTGTCAGCAT+TGG - chr1_2:59559966-59559985 Msa0081050:CDS 40.0%
!! CATGGGTTTTGTGACAAACA+TGG - chr1_2:59559993-59560012 Msa0081050:CDS 40.0%
! GAGGTAAGTATCTGAGATGA+AGG + chr1_2:59560127-59560146 None:intergenic 40.0%
GACATGTGATGAATCTGTTG+AGG + chr1_2:59560146-59560165 None:intergenic 40.0%
CAACAGATTCATCACATGTC+TGG - chr1_2:59560146-59560165 Msa0081050:CDS 40.0%
! TGGCATTGGGATTTGGAATT+TGG + chr1_2:59560236-59560255 None:intergenic 40.0%
! GGTAAATCAAGTTGTGTGGA+AGG - chr1_2:59560521-59560540 Msa0081050:CDS 40.0%
! AAATCAAGTTGTGTGGAAGG+AGG - chr1_2:59560524-59560543 Msa0081050:CDS 40.0%
!! GTGTTTGTATGCTTTGGGAA+TGG - chr1_2:59560574-59560593 Msa0081050:CDS 40.0%
! GCAAGTGCTTTTGCTTCATT+TGG + chr1_2:59560656-59560675 None:intergenic 40.0%
!! GGATCAGTTATTGGTGTTAC+TGG - chr1_2:59560713-59560732 Msa0081050:intron 40.0%
! GGGTTAGTACACAAAAAGCT+TGG - chr1_2:59560744-59560763 Msa0081050:intron 40.0%
GTACACAAAAAGCTTGGCAT+TGG - chr1_2:59560750-59560769 Msa0081050:intron 40.0%
ACACAAAAAGCTTGGCATTG+GGG - chr1_2:59560752-59560771 Msa0081050:intron 40.0%
CTCAAAATAGGACTTTCACC+AGG + chr1_2:59560857-59560876 None:intergenic 40.0%
! CTGGTGAAAGTCCTATTTTG+AGG - chr1_2:59560855-59560874 Msa0081050:intron 40.0%
TGTTCATTAGATTCTGGCAG+TGG + chr1_2:59561006-59561025 None:intergenic 40.0%
TTGCACACACTAACCAAATG+AGG - chr1_2:59561064-59561083 Msa0081050:intron 40.0%
TGATAGTAGGCCCAAATTCT+TGG + chr1_2:59561256-59561275 None:intergenic 40.0%
GTGTAGAAGTGAGTGATAGT+AGG + chr1_2:59561269-59561288 None:intergenic 40.0%
ATCACTCACTTCTACACGTT+CGG - chr1_2:59561272-59561291 Msa0081050:intron 40.0%
ATTTCGTTGTGAAATCAGGG+TGG + chr1_2:59561557-59561576 None:intergenic 40.0%
CCTGATTTCGTTGTGAAATC+AGG + chr1_2:59561561-59561580 None:intergenic 40.0%
CCTGATTTCACAACGAAATC+AGG - chr1_2:59561558-59561577 Msa0081050:intron 40.0%
GTGATTCAAAACACACGCTA+AGG - chr1_2:59561609-59561628 Msa0081050:intron 40.0%
ATTCTGAACCTCAACAACCA+TGG - chr1_2:59561867-59561886 Msa0081050:CDS 40.0%
ATAACAGGAATGGATGATGC+AGG + chr1_2:59562055-59562074 None:intergenic 40.0%
GTTACACAGCTTCAAAGAGT+TGG - chr1_2:59562163-59562182 Msa0081050:intron 40.0%
GCCATTTCAATGACAATAGC+TGG - chr1_2:59562205-59562224 Msa0081050:intron 40.0%
! TTGAAGGGTCTTTTCTTCCT+TGG + chr1_2:59562255-59562274 None:intergenic 40.0%
! ACGTTTTTGTCCATGTCACT+TGG - chr1_2:59562409-59562428 Msa0081050:CDS 40.0%
CAAGAAGTAACCAAGTGACA+TGG + chr1_2:59562422-59562441 None:intergenic 40.0%
AGGATCACACCAAGTAAACA+AGG - chr1_2:59562481-59562500 Msa0081050:CDS 40.0%
TCACACCAAGTAAACAAGGT+TGG - chr1_2:59562485-59562504 Msa0081050:CDS 40.0%
AGGTGAAACACCTCTCTTAA+TGG - chr1_2:59562669-59562688 Msa0081050:CDS 40.0%
GAAACACCTCTCTTAATGGT+TGG - chr1_2:59562673-59562692 Msa0081050:CDS 40.0%
!! CAGTATTGGCTTCACTAGTT+TGG + chr1_2:59562741-59562760 None:intergenic 40.0%
AACTAGTGAAGCCAATACTG+AGG - chr1_2:59562741-59562760 Msa0081050:intron 40.0%
ACTAGTGAAGCCAATACTGA+GGG - chr1_2:59562742-59562761 Msa0081050:intron 40.0%
TTGGATGTGCAAATAGGAAG+TGG - chr1_2:59562883-59562902 Msa0081050:five_prime_UTR 40.0%
!! TACTATTGTTGGTTCTGGCA+CGG - chr1_2:59562953-59562972 Msa0081050:five_prime_UTR 40.0%
ACTTTCTTGCTCTCCCTTGT+TGG - chr1_2:59560099-59560118 Msa0081050:CDS 45.0%
!! TGAGATGAAGGCACCAACAA+GGG + chr1_2:59560115-59560134 None:intergenic 45.0%
! TGGAACATGGCATTGGGATT+TGG + chr1_2:59560243-59560262 None:intergenic 45.0%
!! CACGGTGAATCACTTTTGTC+TGG - chr1_2:59560338-59560357 Msa0081050:CDS 45.0%
GATCACAGTTCAAGCTGCAT+TGG - chr1_2:59560475-59560494 Msa0081050:CDS 45.0%
!! TTGTATGCTTTGGGAATGGG+TGG - chr1_2:59560578-59560597 Msa0081050:CDS 45.0%
TCACTCACTTCTACACGTTC+GGG - chr1_2:59561273-59561292 Msa0081050:intron 45.0%
!!! TTATTTTGAGAGGGTAGGGG+AGG - chr1_2:59561641-59561660 Msa0081050:CDS 45.0%
TGAACCTCAACAACCATGGA+AGG - chr1_2:59561871-59561890 Msa0081050:CDS 45.0%
CAAACCTTCCATGGTTGTTG+AGG + chr1_2:59561878-59561897 None:intergenic 45.0%
AAAATCACTCACCACCCTTC+AGG - chr1_2:59562139-59562158 Msa0081050:intron 45.0%
GAAGCTGTGTAACACCTGAA+GGG + chr1_2:59562156-59562175 None:intergenic 45.0%
TGAAGCTGTGTAACACCTGA+AGG + chr1_2:59562157-59562176 None:intergenic 45.0%
!! CAGCTTCAAAGAGTTGGTGT+TGG - chr1_2:59562169-59562188 Msa0081050:intron 45.0%
GCAGACATGTTCACTCTTGT+TGG - chr1_2:59562325-59562344 Msa0081050:CDS 45.0%
ACACCTCTCTTAATGGTTGG+TGG - chr1_2:59562676-59562695 Msa0081050:CDS 45.0%
CTTCCACCAACCATTAAGAG+AGG + chr1_2:59562682-59562701 None:intergenic 45.0%
ACGGTGAGCACAGAAACCAA+GGG - chr1_2:59559402-59559421 Msa0081050:three_prime_UTR 50.0%
!! CTGAGATGAAGGCACCAACA+AGG + chr1_2:59560116-59560135 None:intergenic 50.0%
TTGGGAATGGGTGGAGTAAG+AGG - chr1_2:59560587-59560606 Msa0081050:CDS 50.0%
TAGGGGAGGTGGAATTGCTA+AGG - chr1_2:59561655-59561674 Msa0081050:CDS 50.0%
GTCACTGTGCAAACCTTCCA+TGG + chr1_2:59561887-59561906 None:intergenic 50.0%
ACTTCCTCTATTGGGCCTCT+TGG - chr1_2:59562590-59562609 Msa0081050:intron 50.0%
TGTACCAAGAGGCCCAATAG+AGG + chr1_2:59562597-59562616 None:intergenic 50.0%
AGAAGAGGGTCCCTCAGTAT+TGG + chr1_2:59562755-59562774 None:intergenic 50.0%
CTCTGTGTGTGCAGAGTCAA+AGG - chr1_2:59562985-59563004 Msa0081050:five_prime_UTR 50.0%
GACGGTGAGCACAGAAACCA+AGG - chr1_2:59559401-59559420 Msa0081050:three_prime_UTR 55.0%
!! TTTTGAGAGGGTAGGGGAGG+TGG - chr1_2:59561644-59561663 Msa0081050:CDS 55.0%
GCTGTGTAACACCTGAAGGG+TGG + chr1_2:59562153-59562172 None:intergenic 55.0%
CAGGGTGGCTCACCGCGATT+AGG + chr1_2:59561542-59561561 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 59559376 59563122 59559376 ID=Msa0081050;Name=Msa0081050
chr1_2 mRNA 59559376 59563122 59559376 ID=Msa0081050-mRNA-1;Parent=Msa0081050;Name=Msa0081050-mRNA-1;_AED=0.06;_eAED=0.06;_QI=323|1|1|1|1|1|6|303|610
chr1_2 exon 59559376 59560663 59559376 ID=Msa0081050-mRNA-1:exon:19270;Parent=Msa0081050-mRNA-1
chr1_2 exon 59561413 59561528 59561413 ID=Msa0081050-mRNA-1:exon:19269;Parent=Msa0081050-mRNA-1
chr1_2 exon 59561612 59562034 59561612 ID=Msa0081050-mRNA-1:exon:19268;Parent=Msa0081050-mRNA-1
chr1_2 exon 59562307 59562524 59562307 ID=Msa0081050-mRNA-1:exon:19267;Parent=Msa0081050-mRNA-1
chr1_2 exon 59562624 59562711 59562624 ID=Msa0081050-mRNA-1:exon:19266;Parent=Msa0081050-mRNA-1
chr1_2 exon 59562797 59563122 59562797 ID=Msa0081050-mRNA-1:exon:19265;Parent=Msa0081050-mRNA-1
chr1_2 five_prime_UTR 59562800 59563122 59562800 ID=Msa0081050-mRNA-1:five_prime_utr;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59562797 59562799 59562797 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59562624 59562711 59562624 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59562307 59562524 59562307 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59561612 59562034 59561612 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59561413 59561528 59561413 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 CDS 59559679 59560663 59559679 ID=Msa0081050-mRNA-1:cds;Parent=Msa0081050-mRNA-1
chr1_2 three_prime_UTR 59559376 59559678 59559376 ID=Msa0081050-mRNA-1:three_prime_utr;Parent=Msa0081050-mRNA-1
Gene Sequence

>Msa0081050

ATGGGAGACAAAGAAGTAAAAGAAGAACTCAAACCCATAATTCAATGGAAGAAACAAAAGGGTGGATTTAGAGCTTCCATGTTCATCTTTGTTTTGTCAGCATTGGATAACATGGGTTTTGTGACAAACATGGTGAGCTTAGTACTATACTTTATTGGAGTGATGCACTTTGATCTTTCAAGCTCTGCCAACACTTTGACAAATTTCATGGGCTCAACTTTCTTGCTCTCCCTTGTTGGTGCCTTCATCTCAGATACTTACCTCAACAGATTCATCACATGTCTGGTTTTTGGATCACTCGAAGTTATTGCTTTGGTAATGATCACAGTTCAAGCTGCATTGGATAATTTACATCCTAAAGCATGTGGTAAATCAAGTTGTGTGGAAGGAGGTATAGCAGTGATGTTTTACTCATCATTGTGTTTGTATGCTTTGGGAATGGGTGGAGTAAGAGGTTCATTAACAGCATTTGGTGCTAACCAATTTGATGAAAAGGATCCAAATGAAGCAAAAGCACTTGCAACTTTTTTCAATTGGCTTTTACTTAGTTCAACTTTGGGATCAGTTATTGGTGTTACTGGTATTGTTTGGGTTAGTACACAAAAAGCTTGGCATTGGGGATTTTTCATCATAACAGTAGCTTCTTCAATTGGTTTTGTAACACTTGCTATTGGTAAACCTTTTTACCGTATCAAAACTCCTGGTGAAAGTCCTATTTTGAGGATTATTCAGGTTATTGTTGTGGCTTTTAAGAATAGAAAGCTACCACTGCCAGAATCTAATGAACAACTTCATGAAGTGTATAAAGATGCTACTTTAGAGAAGATTGCACACACTAACCAAATGAGGTTTCTAGATAAAGCAGCAATTCTTCAAGAAAATTCTGAACCTCAACAACCATGGAAGGTTTGCACAGTGACACAAGTTGAAGAAGTAAAAATCCTAACAAGAATGTTACCTATATTGGCAAGTACAATTGTAATGAACACATGTTTAGCACAACTTCAAACATTCTCAGTACAACAAGGGAATGCAATGAACCTTAAACTTGGTTCTTTCACAGTACCTGCATCATCCATTCCTGTTATCCCACTAATTTTCTTATGCACATTGATTCCAATTTATGAACTTTTCTTTGTACCATTTATTAGAAAAATCACTCACCACCCTTCAGGTGTTACACAGCTTCAAAGAGTTGGTGTTGGACTAGTACTCTCAGCCATTTCAATGACAATAGCTGGTTTTATAGAAGTTAAAAGAAGAGACCAAGGAAGAAAAGACCCTTCAAAACCAATAAGCTTATTTTGGTTATCTTTTCAATATGCTATATTTGGTATTGCAGACATGTTCACTCTTGTTGGACTATTAGAATTCTTCTACAGAGAAGCACCTTCAACCATGAAATCACTTTCTACTTCATTCACGTTTTTGTCCATGTCACTTGGTTACTTCTTGAGTACTGTTTTTGTTAATGTTATTAATACTGTTACTAAAAGGATCACACCAAGTAAACAAGGTTGGTTACAAGGTTTTGATTTAAATCAAAACAATTTGAACTTGTTTTATTGGTTCTTAGCTATCTTAAGCTGTCTTAATTTCTTTAACTTCCTCTATTGGGCCTCTTGGTACAAGTACAAATCAGAAGATAGTAACAATTCAATGATGGAGAATACAAAGGAATTAGGTGAAACACCTCTCTTAATGGTTGGTGGAAGAAAACATGATGATAAAGCTAAGGCAAAAGAAAGTAGCCAAACTAGTGAAGCCAATACTGAGGGACCCTCTTCTTCTGATGAAACAGATGATGGCAAAGAGAAAGAAAGACATAGATGA
Protein sequence

>Msa0081050

MGDKEVKEELKPIIQWKKQKGGFRASMFIFVLSALDNMGFVTNMVSLVLYFIGVMHFDLSSSANTLTNFMGSTFLLSLVGAFISDTYLNRFITCLVFGSLEVIALVMITVQAALDNLHPKACGKSSCVEGGIAVMFYSSLCLYALGMGGVRGSLTAFGANQFDEKDPNEAKALATFFNWLLLSSTLGSVIGVTGIVWVSTQKAWHWGFFIITVASSIGFVTLAIGKPFYRIKTPGESPILRIIQVIVVAFKNRKLPLPESNEQLHEVYKDATLEKIAHTNQMRFLDKAAILQENSEPQQPWKVCTVTQVEEVKILTRMLPILASTIVMNTCLAQLQTFSVQQGNAMNLKLGSFTVPASSIPVIPLIFLCTLIPIYELFFVPFIRKITHHPSGVTQLQRVGVGLVLSAISMTIAGFIEVKRRDQGRKDPSKPISLFWLSFQYAIFGIADMFTLVGLLEFFYREAPSTMKSLSTSFTFLSMSLGYFLSTVFVNVINTVTKRITPSKQGWLQGFDLNQNNLNLFYWFLAILSCLNFFNFLYWASWYKYKSEDSNNSMMENTKELGETPLLMVGGRKHDDKAKAKESSQTSEANTEGPSSSDETDDGKEKERHR*