AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0100850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100850 MtrunA17_Chr1g0148731 88.000 175 8 2 1 174 1 163 2.31e-89 258
Msa0100850 MtrunA17_Chr2g0331271 40.113 177 84 5 6 173 11 174 8.21e-34 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100850 AT4G32590.1 76.515 132 19 1 43 174 54 173 3.74e-59 182
Msa0100850 AT4G32590.3 74.380 121 19 1 43 163 54 162 5.01e-50 159
Msa0100850 AT4G32590.4 78.652 89 19 0 43 131 54 142 2.13e-37 127
Msa0100850 AT3G16250.1 40.476 168 73 5 20 173 30 184 1.75e-31 113
Msa0100850 AT4G32590.5 80.597 67 13 0 43 109 54 120 3.32e-24 92.0
Msa0100850 AT4G32590.2 75.510 49 12 0 43 91 54 102 5.92e-21 83.2

Find 73 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGCGCATTCTCTATTT+TGG 0.194013 1_3:+7281121 None:intergenic
CAAGCCAACCTCCAAGATTC+AGG 0.247976 1_3:+7282265 None:intergenic
TGAATAGAAATATACAATAT+TGG 0.273989 1_3:-7284246 Msa0100850:five_prime_UTR
TTTGGCTCTTGAATTAGATT+TGG 0.280607 1_3:+7281139 None:intergenic
GCGCAAAGCACATTGAATTA+AGG 0.296055 1_3:-7281107 Msa0100850:three_prime_UTR
GAAAATAAATGGTTGGATTC+CGG 0.304867 1_3:+7284087 None:intergenic
ATGAACTCCAGCTCTATTTC+CGG 0.309865 1_3:+7284005 None:intergenic
TGTAAGAATAGTTAGAGTTT+AGG 0.315826 1_3:-7284198 Msa0100850:five_prime_UTR
CATTTGGTTACTATTCTTTA+CGG 0.319773 1_3:-7281204 Msa0100850:three_prime_UTR
TCCGGAGGAGAAGGAGATTC+AGG 0.327786 1_3:+7284023 None:intergenic
ATCATTACAACAATCATATA+AGG 0.333230 1_3:+7281230 None:intergenic
TCGCCGCCGGGGAAAATAAA+TGG 0.351768 1_3:+7284076 None:intergenic
AATGGTTGGATTCCGGTTAC+CGG 0.353946 1_3:+7284094 None:intergenic
AAGTTGATGAACTGCGGTGG+AGG 0.365012 1_3:-7283022 Msa0100850:CDS
AGAGGGTGATGGGTCGTTTC+CGG 0.368856 1_3:-7283875 Msa0100850:CDS
TCCAAGTGAAGTCAGTTTAC+TGG 0.368899 1_3:+7284223 None:intergenic
TTGGCTTGTCAAACTATTGT+AGG 0.372078 1_3:-7282250 Msa0100850:CDS
CGCAAAGCACATTGAATTAA+GGG 0.374261 1_3:-7281106 Msa0100850:three_prime_UTR
ATAAACAGAAACCTGAATCT+TGG 0.374507 1_3:-7282276 Msa0100850:CDS
CAGAGGGCGCGAAGCAGAAA+AGG 0.385776 1_3:-7284162 Msa0100850:five_prime_UTR
TTACCGGCGGTGAGTGGAAC+TGG 0.390189 1_3:+7284110 None:intergenic
AAATAGAGCTGGAGTTCATT+GGG 0.393000 1_3:-7284001 Msa0100850:CDS
GACCCATCACCCTCTGGCTT+TGG 0.402586 1_3:+7283883 None:intergenic
GAAATAGAGCTGGAGTTCAT+TGG 0.409789 1_3:-7284002 Msa0100850:CDS
TTCCACTCACCGCCGGTAAC+CGG 0.413919 1_3:-7284106 Msa0100850:CDS
CTTCGGTTGCAGATCATTGA+AGG 0.429966 1_3:-7282769 Msa0100850:intron
AACTGAGTTCGGTCGCCGCC+GGG 0.457166 1_3:+7284064 None:intergenic
CCAAGCCAAGTTGCACATCC+TGG 0.457293 1_3:-7281346 Msa0100850:three_prime_UTR
GCCTGAATCTCCTTCTCCTC+CGG 0.472889 1_3:-7284024 Msa0100850:CDS
GCAACGAAACAAACTGAGTT+CGG 0.481120 1_3:+7284053 None:intergenic
CCAGGATGTGCAACTTGGCT+TGG 0.484687 1_3:+7281346 None:intergenic
GTATGTTACTTCTTCCATTG+AGG 0.486929 1_3:+7281368 None:intergenic
GCCAGTAAACTGACTTCACT+TGG 0.489799 1_3:-7284224 Msa0100850:five_prime_UTR
CAGCCAAAGCCAGAGGGTGA+TGG 0.497718 1_3:-7283886 Msa0100850:CDS
AGCTCTATTTCCGGAGGAGA+AGG 0.500109 1_3:+7284014 None:intergenic
TTCTCCTCCGGAAATAGAGC+TGG 0.505143 1_3:-7284012 Msa0100850:CDS
AAACTGAGTTCGGTCGCCGC+CGG 0.506246 1_3:+7284063 None:intergenic
ATTGCTTTCACTTTATCCAC+CGG 0.506855 1_3:+7283856 None:intergenic
AAAATCCAGGATGTGCAACT+TGG 0.514003 1_3:+7281341 None:intergenic
AGTTGATGAACTGCGGTGGA+GGG 0.519467 1_3:-7283021 Msa0100850:CDS
GAAACCTGAATCTTGGAGGT+TGG 0.519969 1_3:-7282269 Msa0100850:CDS
TTGTTGTAATGATTGTCATT+TGG 0.523276 1_3:-7281220 Msa0100850:three_prime_UTR
AACAGAAACCTGAATCTTGG+AGG 0.523913 1_3:-7282273 Msa0100850:CDS
TTCCGGTTACCGGCGGTGAG+TGG 0.524356 1_3:+7284104 None:intergenic
ATGCAAACTATATAAAATCC+AGG 0.528301 1_3:+7281328 None:intergenic
TGAACTAAAATATCTTAAGA+AGG 0.529764 1_3:-7282716 Msa0100850:CDS
GGAAACGACCCATCACCCTC+TGG 0.530181 1_3:+7283877 None:intergenic
TTGATGAACTGCGGTGGAGG+GGG 0.531421 1_3:-7283019 Msa0100850:CDS
TTGTTCAAAGGATTCCTCAA+TGG 0.532551 1_3:-7281382 Msa0100850:CDS
AGCCAAAGCCAGAGGGTGAT+GGG 0.532902 1_3:-7283885 Msa0100850:CDS
GCGCGAAGCAGAAAAGGAAA+TGG 0.533002 1_3:-7284156 Msa0100850:exon
GTTGATGAACTGCGGTGGAG+GGG 0.538567 1_3:-7283020 Msa0100850:CDS
TGGCTTGTCAAACTATTGTA+GGG 0.544664 1_3:-7282249 Msa0100850:CDS
GTTGCAGATCATTGAAGGAA+AGG 0.566306 1_3:-7282764 Msa0100850:intron
CGCCGGGGAAAATAAATGGT+TGG 0.580484 1_3:+7284080 None:intergenic
GGGTGATGGGTCGTTTCCGG+TGG 0.591158 1_3:-7283872 Msa0100850:CDS
GTAGGGAACAAAGAAAACTC+TGG 0.594992 1_3:-7282232 Msa0100850:CDS
ACTGAGTTCGGTCGCCGCCG+GGG 0.605244 1_3:+7284065 None:intergenic
ACACCAGTTCCACTCACCGC+CGG 0.614691 1_3:-7284113 Msa0100850:CDS
GAACAAAGAAAACTCTGGCA+AGG 0.616661 1_3:-7282227 Msa0100850:intron
AGGAACACACACACAACAGA+GGG 0.629974 1_3:-7284178 Msa0100850:five_prime_UTR
GGGAAGTTGATGAACTGCGG+TGG 0.636301 1_3:-7283025 Msa0100850:CDS
CTGCGGAACTTGCATAGTCG+AGG 0.643203 1_3:-7282993 Msa0100850:intron
AACTCCAGCTCTATTTCCGG+AGG 0.644295 1_3:+7284008 None:intergenic
TGATTGCAGCCAAAGCCAGA+GGG 0.652551 1_3:-7283892 Msa0100850:intron
GGTTGGATTCCGGTTACCGG+CGG 0.656174 1_3:+7284097 None:intergenic
ATGATTGCAGCCAAAGCCAG+AGG 0.657227 1_3:-7283893 Msa0100850:intron
GCTGATGAGAAGCATCATGT+CGG 0.667602 1_3:-7283821 Msa0100850:CDS
TAGGAACACACACACAACAG+AGG 0.696456 1_3:-7284179 Msa0100850:five_prime_UTR
GATAAAGTGAAAGCAATAAG+CGG 0.697309 1_3:-7283850 Msa0100850:CDS
AATAGAGCTGGAGTTCATTG+GGG 0.704244 1_3:-7284000 Msa0100850:intron
CAGGGGAAGTTGATGAACTG+CGG 0.716036 1_3:-7283028 Msa0100850:CDS
TGGTAGGATACATACATACG+TGG 0.737259 1_3:+7283777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACACAATAAAAAAAAA+CGG + chr1_3:7281722-7281741 None:intergenic 10.0%
!! AATATTTCTTCTATAAAAAA+TGG - chr1_3:7281842-7281861 Msa0100850:intron 10.0%
!! TGAATAGAAATATACAATAT+TGG - chr1_3:7281063-7281082 Msa0100850:three_prime_UTR 15.0%
!!! TTTTAATAGTGTTTGATATA+TGG - chr1_3:7281812-7281831 Msa0100850:intron 15.0%
!! ATTAAATATGAATGTTACAT+TGG - chr1_3:7281950-7281969 Msa0100850:intron 15.0%
!!! CTTTTAAATCTTCTAAAAAA+TGG + chr1_3:7282186-7282205 None:intergenic 15.0%
!!! CATTTTTTAGAAGATTTAAA+AGG - chr1_3:7282184-7282203 Msa0100850:intron 15.0%
!! TCTAATAATAACCATAAAAA+TGG + chr1_3:7282379-7282398 None:intergenic 15.0%
!!! AAGTATTATTATTTCTTTGA+AGG - chr1_3:7282745-7282764 Msa0100850:CDS 15.0%
!! ATTAGCTAATTTCAAAATAT+TGG + chr1_3:7282994-7283013 None:intergenic 15.0%
!! ATATATTATACTTTCTATGA+TGG - chr1_3:7283892-7283911 Msa0100850:intron 15.0%
!! TATATTATACTTTCTATGAT+GGG - chr1_3:7283893-7283912 Msa0100850:intron 15.0%
!!! GCATTGAGATATAATTTTTT+TGG - chr1_3:7281760-7281779 Msa0100850:intron 20.0%
!!! TAGGTTTTTAGATTAATCAT+CGG - chr1_3:7282077-7282096 Msa0100850:intron 20.0%
!!! TTTTTGCAATCTAATTTTGA+CGG - chr1_3:7282252-7282271 Msa0100850:CDS 20.0%
!!! ATCTAAAATTATTCATGTCT+GGG + chr1_3:7282455-7282474 None:intergenic 20.0%
!!! TATCTAAAATTATTCATGTC+TGG + chr1_3:7282456-7282475 None:intergenic 20.0%
!! TGAACTAAAATATCTTAAGA+AGG - chr1_3:7282593-7282612 Msa0100850:intron 20.0%
!! AGAAATGATGATTCTTAAAA+TGG - chr1_3:7282655-7282674 Msa0100850:intron 20.0%
!! TAAAATACTTAGCACATTAT+TGG - chr1_3:7282678-7282697 Msa0100850:intron 20.0%
!!! ATTTTAACACATTGTTTTCA+AGG - chr1_3:7282706-7282725 Msa0100850:intron 20.0%
!!! TTATTATTTCTTTGAAGGTA+TGG - chr1_3:7282750-7282769 Msa0100850:CDS 20.0%
!! AACAACTTTCAGTATATATA+TGG - chr1_3:7283546-7283565 Msa0100850:intron 20.0%
!! TTATGAAACTTGATGAAATA+TGG + chr1_3:7283590-7283609 None:intergenic 20.0%
!! AAATAGACTTAATTCATAGA+AGG + chr1_3:7283671-7283690 None:intergenic 20.0%
!! TTAATTATCATATCATCCAA+AGG + chr1_3:7283700-7283719 None:intergenic 20.0%
!! ATGATATGATAATTAAAGCA+TGG - chr1_3:7283704-7283723 Msa0100850:intron 20.0%
!!! ATTCTAAAATTAATCAAGAC+TGG - chr1_3:7283837-7283856 Msa0100850:CDS 20.0%
!! ATCATTACAACAATCATATA+AGG + chr1_3:7284082-7284101 None:intergenic 20.0%
! TGTAAGAATAGTTAGAGTTT+AGG - chr1_3:7281111-7281130 Msa0100850:three_prime_UTR 25.0%
!! AAAGAGAAAAAGTACATGTA+TGG + chr1_3:7281577-7281596 None:intergenic 25.0%
! GAATTCAGATATCTTAATCA+AGG - chr1_3:7281986-7282005 Msa0100850:intron 25.0%
!! TCATCTTTTAACCAAGTTAT+CGG + chr1_3:7282125-7282144 None:intergenic 25.0%
! AATCATCATTTCTCAAATAC+AGG + chr1_3:7282648-7282667 None:intergenic 25.0%
!! ATAATACTTCTCATTTGCTA+AGG + chr1_3:7282734-7282753 None:intergenic 25.0%
! AGGTTAAATGAGTTAAAGAA+CGG + chr1_3:7282900-7282919 None:intergenic 25.0%
!! TCAACTAAGAACAAATTTTG+AGG - chr1_3:7283326-7283345 Msa0100850:intron 25.0%
!! CAACTAAGAACAAATTTTGA+GGG - chr1_3:7283327-7283346 Msa0100850:intron 25.0%
! AGAACATTGAAACTAGAATA+AGG - chr1_3:7283409-7283428 Msa0100850:intron 25.0%
!! AAACTTAGTAAAAAGTGTAC+CGG + chr1_3:7283499-7283518 None:intergenic 25.0%
!!! ACTTTTTACTAAGTTTGTTC+CGG - chr1_3:7283503-7283522 Msa0100850:intron 25.0%
! ATTAAGTACTATAGTGCTAA+TGG - chr1_3:7283865-7283884 Msa0100850:CDS 25.0%
! ATGCAAACTATATAAAATCC+AGG + chr1_3:7283984-7284003 None:intergenic 25.0%
! TTGTTGTAATGATTGTCATT+TGG - chr1_3:7284089-7284108 Msa0100850:CDS 25.0%
! CATTTGGTTACTATTCTTTA+CGG - chr1_3:7284105-7284124 Msa0100850:CDS 25.0%
! CAAAAATGGCTTATATTACA+GGG + chr1_3:7284246-7284265 None:intergenic 25.0%
GAAAATAAATGGTTGGATTC+CGG + chr1_3:7281225-7281244 None:intergenic 30.0%
GATAAAGTGAAAGCAATAAG+CGG - chr1_3:7281459-7281478 Msa0100850:intron 30.0%
AAAGTACATGTATGGTTGAT+TGG + chr1_3:7281569-7281588 None:intergenic 30.0%
TATCTGAATTCAGTTTCACT+CGG + chr1_3:7281977-7281996 None:intergenic 30.0%
! AGGTCTAAGATTAGAGTATT+TGG - chr1_3:7282006-7282025 Msa0100850:intron 30.0%
! GGTCTAAGATTAGAGTATTT+GGG - chr1_3:7282007-7282026 Msa0100850:intron 30.0%
! GTATTTGGGATGAGAAAATA+TGG - chr1_3:7282021-7282040 Msa0100850:intron 30.0%
TTTCAGATTATGCCAATATC+AGG - chr1_3:7282148-7282167 Msa0100850:intron 30.0%
! ATCCTTTTTGATCCTGATAT+TGG + chr1_3:7282163-7282182 None:intergenic 30.0%
!! ACTAGCATGATCCATTTTTA+TGG - chr1_3:7282365-7282384 Msa0100850:intron 30.0%
CCATATGTAGTATCAATTAG+AGG + chr1_3:7282517-7282536 None:intergenic 30.0%
CCTCTAATTGATACTACATA+TGG - chr1_3:7282514-7282533 Msa0100850:intron 30.0%
GATACTACATATGGATTCTT+CGG - chr1_3:7282523-7282542 Msa0100850:intron 30.0%
! GAAAGGATCTTTTGAATGAA+AGG - chr1_3:7282562-7282581 Msa0100850:intron 30.0%
!!! TTTTAACTGATTTGCGCTAA+AGG + chr1_3:7282920-7282939 None:intergenic 30.0%
ATAAACAGAAACCTGAATCT+TGG - chr1_3:7283033-7283052 Msa0100850:intron 30.0%
ACTATATCTCATGTTTAGTG+TGG - chr1_3:7283165-7283184 Msa0100850:intron 30.0%
CTTATATGTAACTATCACTC+TGG + chr1_3:7283202-7283221 None:intergenic 30.0%
CTCACATTATTGAGAATTGA+AGG - chr1_3:7283254-7283273 Msa0100850:intron 30.0%
AAGTCTATTTACTTGACCTT+TGG - chr1_3:7283681-7283700 Msa0100850:intron 30.0%
!!! TAGTCACTTTTGTTTGCATT+AGG + chr1_3:7283764-7283783 None:intergenic 30.0%
AATGCAAACAAAAGTGACTA+CGG - chr1_3:7283764-7283783 Msa0100850:intron 30.0%
TTGAAGAGAACCATACAAAT+TGG + chr1_3:7283798-7283817 None:intergenic 30.0%
!! TTTGGCTCTTGAATTAGATT+TGG + chr1_3:7284173-7284192 None:intergenic 30.0%
CCAAAAATGGCTTATATTAC+AGG + chr1_3:7284247-7284266 None:intergenic 30.0%
!! CCTGTAATATAAGCCATTTT+TGG - chr1_3:7284244-7284263 Msa0100850:five_prime_UTR 30.0%
!! ATCCAACCATTTATTTTCCC+CGG - chr1_3:7281227-7281246 Msa0100850:three_prime_UTR 35.0%
AAATAGAGCTGGAGTTCATT+GGG - chr1_3:7281308-7281327 Msa0100850:three_prime_UTR 35.0%
ATTGCTTTCACTTTATCCAC+CGG + chr1_3:7281456-7281475 None:intergenic 35.0%
ATTGGAATTAAGCATGTGGT+AGG + chr1_3:7281551-7281570 None:intergenic 35.0%
GTTGATTGGAATTAAGCATG+TGG + chr1_3:7281555-7281574 None:intergenic 35.0%
TTGGGATGAGAAAATATGGT+TGG - chr1_3:7282025-7282044 Msa0100850:intron 35.0%
TGGGATGAGAAAATATGGTT+GGG - chr1_3:7282026-7282045 Msa0100850:intron 35.0%
AGTAAAGAGCTCGTTAAAGA+TGG - chr1_3:7282049-7282068 Msa0100850:intron 35.0%
CTCGTTAAAGATGGTTAACT+AGG - chr1_3:7282058-7282077 Msa0100850:intron 35.0%
GATACTTCTTACCGATAACT+TGG - chr1_3:7282111-7282130 Msa0100850:intron 35.0%
TGCCAATATCAGGATCAAAA+AGG - chr1_3:7282158-7282177 Msa0100850:intron 35.0%
!! TAATTTTGACGGTCACAACA+GGG - chr1_3:7282263-7282282 Msa0100850:CDS 35.0%
GGTTTGAAAATGAGTTCGAT+TGG + chr1_3:7282780-7282799 None:intergenic 35.0%
CTACATCATTAGCATCAGAT+AGG + chr1_3:7282801-7282820 None:intergenic 35.0%
AATGTTCCTTTCATACTGTG+AGG - chr1_3:7282958-7282977 Msa0100850:intron 35.0%
ATGTTCCTTTCATACTGTGA+GGG - chr1_3:7282959-7282978 Msa0100850:intron 35.0%
!! TTGGCTTGTCAAACTATTGT+AGG - chr1_3:7283059-7283078 Msa0100850:intron 35.0%
! TGGCTTGTCAAACTATTGTA+GGG - chr1_3:7283060-7283079 Msa0100850:intron 35.0%
GGCTTACACAATACTCAATA+CGG - chr1_3:7283348-7283367 Msa0100850:intron 35.0%
GGAATCTTCTCCAATTTGTA+TGG - chr1_3:7283785-7283804 Msa0100850:intron 35.0%
!!! ACTTTCTATGATGGGCTTTT+AGG - chr1_3:7283901-7283920 Msa0100850:intron 35.0%
!!! GCTTTTAGGTTGTTGTTCAA+AGG - chr1_3:7283915-7283934 Msa0100850:intron 35.0%
TTGTTCAAAGGATTCCTCAA+TGG - chr1_3:7283927-7283946 Msa0100850:intron 35.0%
! GTATGTTACTTCTTCCATTG+AGG + chr1_3:7283944-7283963 None:intergenic 35.0%
CGCAAAGCACATTGAATTAA+GGG - chr1_3:7284203-7284222 Msa0100850:five_prime_UTR 35.0%
TCCAAGTGAAGTCAGTTTAC+TGG + chr1_3:7281089-7281108 None:intergenic 40.0%
GCAACGAAACAAACTGAGTT+CGG + chr1_3:7281259-7281278 None:intergenic 40.0%
ATGAACTCCAGCTCTATTTC+CGG + chr1_3:7281307-7281326 None:intergenic 40.0%
GAAATAGAGCTGGAGTTCAT+TGG - chr1_3:7281307-7281326 Msa0100850:three_prime_UTR 40.0%
AATAGAGCTGGAGTTCATTG+GGG - chr1_3:7281309-7281328 Msa0100850:three_prime_UTR 40.0%
TGGTAGGATACATACATACG+TGG + chr1_3:7281535-7281554 None:intergenic 40.0%
!! CTAATTTTGACGGTCACAAC+AGG - chr1_3:7282262-7282281 Msa0100850:CDS 40.0%
!! AATTTTGACGGTCACAACAG+GGG - chr1_3:7282264-7282283 Msa0100850:CDS 40.0%
GTTGCAGATCATTGAAGGAA+AGG - chr1_3:7282545-7282564 Msa0100850:intron 40.0%
TACTACCCTCACAGTATGAA+AGG + chr1_3:7282967-7282986 None:intergenic 40.0%
AACAGAAACCTGAATCTTGG+AGG - chr1_3:7283036-7283055 Msa0100850:intron 40.0%
GTAGGGAACAAAGAAAACTC+TGG - chr1_3:7283077-7283096 Msa0100850:intron 40.0%
GAACAAAGAAAACTCTGGCA+AGG - chr1_3:7283082-7283101 Msa0100850:intron 40.0%
CATGTTTAGTGTGGCAAATG+TGG - chr1_3:7283174-7283193 Msa0100850:intron 40.0%
! GTGTAATGAGTTGAAGAGAC+TGG + chr1_3:7283293-7283312 None:intergenic 40.0%
CGGACAATACCTTAGAAAAG+TGG - chr1_3:7283368-7283387 Msa0100850:intron 40.0%
!! TCTGAGACACCACTTTTCTA+AGG + chr1_3:7283380-7283399 None:intergenic 40.0%
AAAGTGGTGTCTCAGATTTG+AGG - chr1_3:7283384-7283403 Msa0100850:intron 40.0%
! TTTTAGCCCCATAGGCTAAA+GGG - chr1_3:7283462-7283481 Msa0100850:intron 40.0%
! TTACTAAGTTTGTTCCGGTG+TGG - chr1_3:7283508-7283527 Msa0100850:intron 40.0%
AAAATCCAGGATGTGCAACT+TGG + chr1_3:7283971-7283990 None:intergenic 40.0%
! GCTTTGCGCATTCTCTATTT+TGG + chr1_3:7284191-7284210 None:intergenic 40.0%
GCGCAAAGCACATTGAATTA+AGG - chr1_3:7284202-7284221 Msa0100850:five_prime_UTR 40.0%
TTGGGGGTGAAAACCAAAAA+TGG + chr1_3:7284260-7284279 None:intergenic 40.0%
! GCCAGTAAACTGACTTCACT+TGG - chr1_3:7281085-7281104 Msa0100850:three_prime_UTR 45.0%
TAGGAACACACACACAACAG+AGG - chr1_3:7281130-7281149 Msa0100850:three_prime_UTR 45.0%
AGGAACACACACACAACAGA+GGG - chr1_3:7281131-7281150 Msa0100850:three_prime_UTR 45.0%
AATGGTTGGATTCCGGTTAC+CGG + chr1_3:7281218-7281237 None:intergenic 45.0%
!! CAACCATTTATTTTCCCCGG+CGG - chr1_3:7281230-7281249 Msa0100850:three_prime_UTR 45.0%
!! GCTGATGAGAAGCATCATGT+CGG - chr1_3:7281488-7281507 Msa0100850:intron 45.0%
CTTCGGTTGCAGATCATTGA+AGG - chr1_3:7282540-7282559 Msa0100850:intron 45.0%
GAAACCTGAATCTTGGAGGT+TGG - chr1_3:7283040-7283059 Msa0100850:intron 45.0%
CTTTAGCCTATGGGGCTAAA+AGG + chr1_3:7283463-7283482 None:intergenic 45.0%
! CTTTTAGCCCCATAGGCTAA+AGG - chr1_3:7283461-7283480 Msa0100850:intron 45.0%
ATAGCCTCCCTTTAGCCTAT+GGG + chr1_3:7283472-7283491 None:intergenic 45.0%
AATAGCCTCCCTTTAGCCTA+TGG + chr1_3:7283473-7283492 None:intergenic 45.0%
CTAAAGGGAGGCTATTGTTC+CGG - chr1_3:7283477-7283496 Msa0100850:intron 45.0%
!! AAAATATTCATTAAATAATT+CGG - chr1_3:7281634-7281653 Msa0100850:intron 5.0%
GCGCGAAGCAGAAAAGGAAA+TGG - chr1_3:7281153-7281172 Msa0100850:three_prime_UTR 50.0%
CGCCGGGGAAAATAAATGGT+TGG + chr1_3:7281232-7281251 None:intergenic 50.0%
AGCTCTATTTCCGGAGGAGA+AGG + chr1_3:7281298-7281317 None:intergenic 50.0%
TTCTCCTCCGGAAATAGAGC+TGG - chr1_3:7281297-7281316 Msa0100850:three_prime_UTR 50.0%
AACTCCAGCTCTATTTCCGG+AGG + chr1_3:7281304-7281323 None:intergenic 50.0%
ATGATTGCAGCCAAAGCCAG+AGG - chr1_3:7281416-7281435 Msa0100850:intron 50.0%
TGATTGCAGCCAAAGCCAGA+GGG - chr1_3:7281417-7281436 Msa0100850:intron 50.0%
!! CAGGGGAAGTTGATGAACTG+CGG - chr1_3:7282281-7282300 Msa0100850:CDS 50.0%
!! AAGTTGATGAACTGCGGTGG+AGG - chr1_3:7282287-7282306 Msa0100850:CDS 50.0%
!! AGTTGATGAACTGCGGTGGA+GGG - chr1_3:7282288-7282307 Msa0100850:CDS 50.0%
CAAGCCAACCTCCAAGATTC+AGG + chr1_3:7283047-7283066 None:intergenic 50.0%
! GATAGGCCTTTTAGCCCCAT+AGG - chr1_3:7283454-7283473 Msa0100850:intron 50.0%
TAGCCTCCCTTTAGCCTATG+GGG + chr1_3:7283471-7283490 None:intergenic 50.0%
TCGCCGCCGGGGAAAATAAA+TGG + chr1_3:7281236-7281255 None:intergenic 55.0%
GCCTGAATCTCCTTCTCCTC+CGG - chr1_3:7281285-7281304 Msa0100850:three_prime_UTR 55.0%
TCCGGAGGAGAAGGAGATTC+AGG + chr1_3:7281289-7281308 None:intergenic 55.0%
AGCCAAAGCCAGAGGGTGAT+GGG - chr1_3:7281424-7281443 Msa0100850:intron 55.0%
!! AGAGGGTGATGGGTCGTTTC+CGG - chr1_3:7281434-7281453 Msa0100850:intron 55.0%
!! GGGAAGTTGATGAACTGCGG+TGG - chr1_3:7282284-7282303 Msa0100850:CDS 55.0%
! GTTGATGAACTGCGGTGGAG+GGG - chr1_3:7282289-7282308 Msa0100850:CDS 55.0%
TTGATGAACTGCGGTGGAGG+GGG - chr1_3:7282290-7282309 Msa0100850:CDS 55.0%
CTGCGGAACTTGCATAGTCG+AGG - chr1_3:7282316-7282335 Msa0100850:intron 55.0%
AGGACATAAGCCACACGCGA+TGG - chr1_3:7283429-7283448 Msa0100850:intron 55.0%
TAGCCCCATAGGCTAAAGGG+AGG - chr1_3:7283465-7283484 Msa0100850:intron 55.0%
TGAACTCGAGTCTGCCACAC+CGG + chr1_3:7283525-7283544 None:intergenic 55.0%
CCAGGATGTGCAACTTGGCT+TGG + chr1_3:7283966-7283985 None:intergenic 55.0%
CCAAGCCAAGTTGCACATCC+TGG - chr1_3:7283963-7283982 Msa0100850:intron 55.0%
! CAGAGGGCGCGAAGCAGAAA+AGG - chr1_3:7281147-7281166 Msa0100850:three_prime_UTR 60.0%
ACACCAGTTCCACTCACCGC+CGG - chr1_3:7281196-7281215 Msa0100850:three_prime_UTR 60.0%
TTACCGGCGGTGAGTGGAAC+TGG + chr1_3:7281202-7281221 None:intergenic 60.0%
TTCCACTCACCGCCGGTAAC+CGG - chr1_3:7281203-7281222 Msa0100850:three_prime_UTR 60.0%
GGTTGGATTCCGGTTACCGG+CGG + chr1_3:7281215-7281234 None:intergenic 60.0%
AAACTGAGTTCGGTCGCCGC+CGG + chr1_3:7281249-7281268 None:intergenic 60.0%
CAGCCAAAGCCAGAGGGTGA+TGG - chr1_3:7281423-7281442 Msa0100850:intron 60.0%
! GACCCATCACCCTCTGGCTT+TGG + chr1_3:7281429-7281448 None:intergenic 60.0%
GGAAACGACCCATCACCCTC+TGG + chr1_3:7281435-7281454 None:intergenic 60.0%
AGCCACACGCGATGGTCGAT+AGG - chr1_3:7283437-7283456 Msa0100850:intron 60.0%
TTCCGGTTACCGGCGGTGAG+TGG + chr1_3:7281208-7281227 None:intergenic 65.0%
AACTGAGTTCGGTCGCCGCC+GGG + chr1_3:7281248-7281267 None:intergenic 65.0%
!! GGGTGATGGGTCGTTTCCGG+TGG - chr1_3:7281437-7281456 Msa0100850:intron 65.0%
GGCCTATCGACCATCGCGTG+TGG + chr1_3:7283442-7283461 None:intergenic 65.0%
ACTGAGTTCGGTCGCCGCCG+GGG + chr1_3:7281247-7281266 None:intergenic 70.0%
TGCGGTGGAGGGGGTAGCTG+CGG - chr1_3:7282299-7282318 Msa0100850:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 7281042 7284289 7281042 ID=Msa0100850;Name=Msa0100850
chr1_3 mRNA 7281042 7284289 7281042 ID=Msa0100850-mRNA-1;Parent=Msa0100850;Name=Msa0100850-mRNA-1;_AED=0.16;_eAED=0.16;_QI=130|0.8|0.66|1|0.2|0.33|6|331|174
chr1_3 exon 7281042 7281408 7281042 ID=Msa0100850-mRNA-1:exon:2856;Parent=Msa0100850-mRNA-1
chr1_3 exon 7282228 7282322 7282228 ID=Msa0100850-mRNA-1:exon:2855;Parent=Msa0100850-mRNA-1
chr1_3 exon 7282713 7282779 7282713 ID=Msa0100850-mRNA-1:exon:2854;Parent=Msa0100850-mRNA-1
chr1_3 exon 7282994 7283047 7282994 ID=Msa0100850-mRNA-1:exon:2853;Parent=Msa0100850-mRNA-1
chr1_3 exon 7283792 7283905 7283792 ID=Msa0100850-mRNA-1:exon:2852;Parent=Msa0100850-mRNA-1
chr1_3 exon 7284001 7284289 7284001 ID=Msa0100850-mRNA-1:exon:2851;Parent=Msa0100850-mRNA-1
chr1_3 five_prime_UTR 7284160 7284289 7284160 ID=Msa0100850-mRNA-1:five_prime_utr;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7284001 7284159 7284001 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7283792 7283905 7283792 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7282994 7283047 7282994 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7282713 7282779 7282713 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7282228 7282322 7282228 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 CDS 7281373 7281408 7281373 ID=Msa0100850-mRNA-1:cds;Parent=Msa0100850-mRNA-1
chr1_3 three_prime_UTR 7281042 7281372 7281042 ID=Msa0100850-mRNA-1:three_prime_utr;Parent=Msa0100850-mRNA-1
Gene Sequence

>Msa0100850

ATGGCTTCTATGAACAATATATTCACACCAGTTCCACTCACCGCCGGTAACCGGAATCCAACCATTTATTTTCCCCGGCGGCGACCGAACTCAGTTTGTTTCGTTGCAAATTCGCCTGAATCTCCTTCTCCTCCGGAAATAGAGCTGGAGTTCATTGGGCCAAAGCCAGAGGGTGATGGGTCGTTTCCGGTGGATAAAGTGAAAGCAATAAGCGGAGAGAAGCTGATGAGAAGCATCATGTCGGATAACAAGATTGAGCTTTATGCCACGTATGGGAAGTTGATGAACTGCGGTGGAGGGGGTAGCTGCGGAACTTGCATAGTCGAGATCATTGAAGGAAAGGATCTTTTGAATGAAAGGACAAATACTGAACTAAAATATCTTAAGAAGGCATCTAATCTTTTGAGCTTCTGCGTTTATAAACAGAAACCTGAATCTTGGAGGTTGGCTTGTCAAACTATTGTAGGGAACAAAGAAAACTCTGGCAAGGTTGTTGTTCAAAGGATTCCTCAATGGAAGAAGTAA
Protein sequence

>Msa0100850

MASMNNIFTPVPLTAGNRNPTIYFPRRRPNSVCFVANSPESPSPPEIELEFIGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIELYATYGKLMNCGGGGSCGTCIVEIIEGKDLLNERTNTELKYLKKASNLLSFCVYKQKPESWRLACQTIVGNKENSGKVVVQRIPQWKK*