AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0101070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101070 MtrunA17_Chr1g0149381 95.082 61 3 0 7 67 31 91 1.43e-34 125
Msa0101070 MtrunA17_Chr3g0137741 63.333 60 22 0 8 67 27 86 5.76e-21 86.3
Msa0101070 MtrunA17_Chr2g0318181 61.667 60 23 0 9 68 32 91 5.58e-18 77.4
Msa0101070 MtrunA17_Chr2g0318171 66.667 57 19 0 9 65 31 87 5.87e-18 77.8
Msa0101070 MtrunA17_Chr2g0314611 58.333 60 25 0 9 68 31 90 1.78e-16 73.6
Msa0101070 MtrunA17_Chr2g0333091 45.714 70 32 2 10 74 27 95 1.78e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101070 AT4G32300.1 60.656 61 24 0 7 67 21 81 1.10e-18 80.1

Find 17 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTCAAACAAACAAGAATT+TGG 0.221242 1_3:-7564250 Msa0101070:CDS
AACAAACAAGAATTTGGTTT+TGG 0.272697 1_3:-7564244 Msa0101070:CDS
TTGAAGGCTCTCAAATGAAT+TGG 0.329811 1_3:-7564297 Msa0101070:CDS
ATGGATAGCACAAAATTGTT+TGG 0.353260 1_3:-7564169 Msa0101070:CDS
TAAGTTTGTGTTTGATGAAA+AGG 0.406184 1_3:-7564111 Msa0101070:CDS
CTCAAATGAATTGGATTGAT+AGG 0.408242 1_3:-7564288 Msa0101070:CDS
TTCGCTGGTATTCAACGGAT+TGG 0.429538 1_3:-7564337 Msa0101070:CDS
ATTTGAGAGCCTTCAAATCC+TGG 0.434231 1_3:+7564304 None:intergenic
AACTTATCAGAATTAGAAAC+AGG 0.475908 1_3:+7564128 None:intergenic
AAGTTTGTGTTTGATGAAAA+GGG 0.485126 1_3:-7564110 Msa0101070:CDS
AAGATCTCACCAGGATTTGA+AGG 0.486645 1_3:-7564313 Msa0101070:CDS
CGGATTGGAAAGATCTCACC+AGG 0.530461 1_3:-7564322 Msa0101070:CDS
ATGAATTGGATTGATAGGAA+TGG 0.530746 1_3:-7564283 Msa0101070:CDS
TTGGTTTCGCTGGTATTCAA+CGG 0.549301 1_3:-7564342 Msa0101070:CDS
GTTTGGTCAACAAATACAAG+TGG 0.585004 1_3:-7564056 Msa0101070:CDS
AAGACTCATTCATCATGTTG+AGG 0.642032 1_3:-7564997 None:intergenic
TCTACTTGCAATAGTCCACA+TGG 0.643271 1_3:-7564188 Msa0101070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACAAAAACAACTTTTTA+AGG + chr1_3:7564292-7564311 None:intergenic 15.0%
!!! AAAACAAAAACAACTTTTTA+AGG + chr1_3:7564292-7564311 None:intergenic 15.0%
!! TTTACAAAAAGGAAGAAATT+TGG - chr1_3:7564956-7564975 Msa0101070:intron 20.0%
!! TTTACAAAAAGGAAGAAATT+TGG - chr1_3:7564956-7564975 Msa0101070:intron 20.0%
! TTCATGAATATGATAGAAGA+GGG + chr1_3:7564209-7564228 None:intergenic 25.0%
! ATTCATGAATATGATAGAAG+AGG + chr1_3:7564210-7564229 None:intergenic 25.0%
! ATATGGCATAGACAAAAAAA+TGG + chr1_3:7564234-7564253 None:intergenic 25.0%
!!! ACAAAAACAACTTTTTAAGG+TGG + chr1_3:7564289-7564308 None:intergenic 25.0%
!! AATTACTTGGTTCTTATATG+AGG + chr1_3:7564419-7564438 None:intergenic 25.0%
!!! TCTTATCTGAGGATTTTTTT+CGG + chr1_3:7564562-7564581 None:intergenic 25.0%
! AAAAATCCTCAGATAAGAAT+TGG - chr1_3:7564564-7564583 Msa0101070:intron 25.0%
!!! AACAAACAAGAATTTGGTTT+TGG - chr1_3:7564790-7564809 Msa0101070:intron 25.0%
! AACTTATCAGAATTAGAAAC+AGG + chr1_3:7564909-7564928 None:intergenic 25.0%
! TAAGTTTGTGTTTGATGAAA+AGG - chr1_3:7564923-7564942 Msa0101070:intron 25.0%
! AAGTTTGTGTTTGATGAAAA+GGG - chr1_3:7564924-7564943 Msa0101070:intron 25.0%
!!! GGTAATGCTTTTTTACAAAA+AGG - chr1_3:7564945-7564964 Msa0101070:intron 25.0%
! AAAAAGGAAGAAATTTGGTT+TGG - chr1_3:7564961-7564980 Msa0101070:intron 25.0%
! TTCATGAATATGATAGAAGA+GGG + chr1_3:7564209-7564228 None:intergenic 25.0%
! ATTCATGAATATGATAGAAG+AGG + chr1_3:7564210-7564229 None:intergenic 25.0%
! ATATGGCATAGACAAAAAAA+TGG + chr1_3:7564234-7564253 None:intergenic 25.0%
!!! ACAAAAACAACTTTTTAAGG+TGG + chr1_3:7564289-7564308 None:intergenic 25.0%
!! AATTACTTGGTTCTTATATG+AGG + chr1_3:7564419-7564438 None:intergenic 25.0%
!!! TCTTATCTGAGGATTTTTTT+CGG + chr1_3:7564562-7564581 None:intergenic 25.0%
! AAAAATCCTCAGATAAGAAT+TGG - chr1_3:7564564-7564583 Msa0101070:intron 25.0%
!!! AACAAACAAGAATTTGGTTT+TGG - chr1_3:7564790-7564809 Msa0101070:intron 25.0%
! AACTTATCAGAATTAGAAAC+AGG + chr1_3:7564909-7564928 None:intergenic 25.0%
! TAAGTTTGTGTTTGATGAAA+AGG - chr1_3:7564923-7564942 Msa0101070:intron 25.0%
! AAGTTTGTGTTTGATGAAAA+GGG - chr1_3:7564924-7564943 Msa0101070:intron 25.0%
!!! GGTAATGCTTTTTTACAAAA+AGG - chr1_3:7564945-7564964 Msa0101070:intron 25.0%
! AAAAAGGAAGAAATTTGGTT+TGG - chr1_3:7564961-7564980 Msa0101070:intron 25.0%
AGAAAAGACAGAAAAGAAAG+TGG + chr1_3:7564132-7564151 None:intergenic 30.0%
! TACATAGTGGAAAAGCAATA+TGG + chr1_3:7564166-7564185 None:intergenic 30.0%
AGGGTTGAATTATGTTATCT+TGG + chr1_3:7564190-7564209 None:intergenic 30.0%
! CACATAGTGGAAAAACAATA+TGG + chr1_3:7564251-7564270 None:intergenic 30.0%
!! CATATTGTTTTTCCACTATG+TGG - chr1_3:7564249-7564268 Msa0101070:CDS 30.0%
AGAAGCAAAGCAAAAAACTA+TGG + chr1_3:7564380-7564399 None:intergenic 30.0%
AGAACCAAGTAATTTGACAA+AGG - chr1_3:7564425-7564444 Msa0101070:intron 30.0%
GAACCAAGTAATTTGACAAA+GGG - chr1_3:7564426-7564445 Msa0101070:intron 30.0%
AATTCTAGTACTTCTATGTG+AGG + chr1_3:7564483-7564502 None:intergenic 30.0%
!!! TTTTTTTCGGTCTTAGAGTT+CGG + chr1_3:7564549-7564568 None:intergenic 30.0%
ACAAAACCATGAGAAATCAT+TGG - chr1_3:7564603-7564622 Msa0101070:intron 30.0%
GTATTCACAATATGGAAAAG+AGG + chr1_3:7564629-7564648 None:intergenic 30.0%
GATACACAAACATAACCATA+GGG + chr1_3:7564651-7564670 None:intergenic 30.0%
TGATACACAAACATAACCAT+AGG + chr1_3:7564652-7564671 None:intergenic 30.0%
!! ACTTTTGTCTGAAGTAGTTT+TGG - chr1_3:7564673-7564692 Msa0101070:intron 30.0%
CTCAAATGAATTGGATTGAT+AGG - chr1_3:7564746-7564765 Msa0101070:intron 30.0%
ATGAATTGGATTGATAGGAA+TGG - chr1_3:7564751-7564770 Msa0101070:intron 30.0%
GTGTCAAACAAACAAGAATT+TGG - chr1_3:7564784-7564803 Msa0101070:intron 30.0%
ATGGATAGCACAAAATTGTT+TGG - chr1_3:7564865-7564884 Msa0101070:intron 30.0%
AGAAAAGACAGAAAAGAAAG+TGG + chr1_3:7564132-7564151 None:intergenic 30.0%
! TACATAGTGGAAAAGCAATA+TGG + chr1_3:7564166-7564185 None:intergenic 30.0%
AGGGTTGAATTATGTTATCT+TGG + chr1_3:7564190-7564209 None:intergenic 30.0%
! CACATAGTGGAAAAACAATA+TGG + chr1_3:7564251-7564270 None:intergenic 30.0%
!! CATATTGTTTTTCCACTATG+TGG - chr1_3:7564249-7564268 Msa0101070:CDS 30.0%
AGAAGCAAAGCAAAAAACTA+TGG + chr1_3:7564380-7564399 None:intergenic 30.0%
AGAACCAAGTAATTTGACAA+AGG - chr1_3:7564425-7564444 Msa0101070:intron 30.0%
GAACCAAGTAATTTGACAAA+GGG - chr1_3:7564426-7564445 Msa0101070:intron 30.0%
AATTCTAGTACTTCTATGTG+AGG + chr1_3:7564483-7564502 None:intergenic 30.0%
!!! TTTTTTTCGGTCTTAGAGTT+CGG + chr1_3:7564549-7564568 None:intergenic 30.0%
ACAAAACCATGAGAAATCAT+TGG - chr1_3:7564603-7564622 Msa0101070:intron 30.0%
GTATTCACAATATGGAAAAG+AGG + chr1_3:7564629-7564648 None:intergenic 30.0%
GATACACAAACATAACCATA+GGG + chr1_3:7564651-7564670 None:intergenic 30.0%
TGATACACAAACATAACCAT+AGG + chr1_3:7564652-7564671 None:intergenic 30.0%
!! ACTTTTGTCTGAAGTAGTTT+TGG - chr1_3:7564673-7564692 Msa0101070:intron 30.0%
CTCAAATGAATTGGATTGAT+AGG - chr1_3:7564746-7564765 Msa0101070:intron 30.0%
ATGAATTGGATTGATAGGAA+TGG - chr1_3:7564751-7564770 Msa0101070:intron 30.0%
GTGTCAAACAAACAAGAATT+TGG - chr1_3:7564784-7564803 Msa0101070:intron 30.0%
ATGGATAGCACAAAATTGTT+TGG - chr1_3:7564865-7564884 Msa0101070:intron 30.0%
ATGTTGAGGTAAACCTTAGT+TGG - chr1_3:7564051-7564070 Msa0101070:CDS 35.0%
ACACTTGAATTGACCAACTA+AGG + chr1_3:7564067-7564086 None:intergenic 35.0%
ATGTTATCTTGGCTACATAG+TGG + chr1_3:7564179-7564198 None:intergenic 35.0%
GAATTCATGAAGAGAGAAGA+GGG + chr1_3:7564358-7564377 None:intergenic 35.0%
TCACCCTTTGTCAAATTACT+TGG + chr1_3:7564432-7564451 None:intergenic 35.0%
ACCTTGACTGAAACAATTCT+CGG - chr1_3:7564506-7564525 Msa0101070:intron 35.0%
TCCATATTGTGAATACCCTA+TGG - chr1_3:7564633-7564652 Msa0101070:intron 35.0%
! ACCATAGGGTATTCACAATA+TGG + chr1_3:7564637-7564656 None:intergenic 35.0%
TTGAAGGCTCTCAAATGAAT+TGG - chr1_3:7564737-7564756 Msa0101070:intron 35.0%
! ACAATTTTGTGCTATCCATG+TGG + chr1_3:7564864-7564883 None:intergenic 35.0%
GTTTGGTCAACAAATACAAG+TGG - chr1_3:7564978-7564997 Msa0101070:intron 35.0%
ATGTTGAGGTAAACCTTAGT+TGG - chr1_3:7564051-7564070 Msa0101070:CDS 35.0%
ACACTTGAATTGACCAACTA+AGG + chr1_3:7564067-7564086 None:intergenic 35.0%
ATGTTATCTTGGCTACATAG+TGG + chr1_3:7564179-7564198 None:intergenic 35.0%
GAATTCATGAAGAGAGAAGA+GGG + chr1_3:7564358-7564377 None:intergenic 35.0%
TCACCCTTTGTCAAATTACT+TGG + chr1_3:7564432-7564451 None:intergenic 35.0%
ACCTTGACTGAAACAATTCT+CGG - chr1_3:7564506-7564525 Msa0101070:intron 35.0%
TCCATATTGTGAATACCCTA+TGG - chr1_3:7564633-7564652 Msa0101070:intron 35.0%
! ACCATAGGGTATTCACAATA+TGG + chr1_3:7564637-7564656 None:intergenic 35.0%
TTGAAGGCTCTCAAATGAAT+TGG - chr1_3:7564737-7564756 Msa0101070:intron 35.0%
! ACAATTTTGTGCTATCCATG+TGG + chr1_3:7564864-7564883 None:intergenic 35.0%
GTTTGGTCAACAAATACAAG+TGG - chr1_3:7564978-7564997 Msa0101070:intron 35.0%
! GTTTTTCCACTATGTGGTCA+AGG - chr1_3:7564255-7564274 Msa0101070:CDS 40.0%
TTTCTACCTTGACCACATAG+TGG + chr1_3:7564264-7564283 None:intergenic 40.0%
AGAGAGAAGAGGGTTGAAAT+AGG + chr1_3:7564348-7564367 None:intergenic 40.0%
GGAATTCATGAAGAGAGAAG+AGG + chr1_3:7564359-7564378 None:intergenic 40.0%
ACCGAGAATTGTTTCAGTCA+AGG + chr1_3:7564510-7564529 None:intergenic 40.0%
CAACAGCCAATTCTTATCTG+AGG + chr1_3:7564573-7564592 None:intergenic 40.0%
CTCAGATAAGAATTGGCTGT+TGG - chr1_3:7564571-7564590 Msa0101070:intron 40.0%
AAGAGGCCAATGATTTCTCA+TGG + chr1_3:7564612-7564631 None:intergenic 40.0%
!!! TGAAGTAGTTTTGGTTTCGC+TGG - chr1_3:7564682-7564701 Msa0101070:intron 40.0%
TTGGTTTCGCTGGTATTCAA+CGG - chr1_3:7564692-7564711 Msa0101070:intron 40.0%
AAGATCTCACCAGGATTTGA+AGG - chr1_3:7564721-7564740 Msa0101070:intron 40.0%
ATTTGAGAGCCTTCAAATCC+TGG + chr1_3:7564733-7564752 None:intergenic 40.0%
TCTACTTGCAATAGTCCACA+TGG - chr1_3:7564846-7564865 Msa0101070:intron 40.0%
! GTTTTTCCACTATGTGGTCA+AGG - chr1_3:7564255-7564274 Msa0101070:CDS 40.0%
TTTCTACCTTGACCACATAG+TGG + chr1_3:7564264-7564283 None:intergenic 40.0%
AGAGAGAAGAGGGTTGAAAT+AGG + chr1_3:7564348-7564367 None:intergenic 40.0%
GGAATTCATGAAGAGAGAAG+AGG + chr1_3:7564359-7564378 None:intergenic 40.0%
ACCGAGAATTGTTTCAGTCA+AGG + chr1_3:7564510-7564529 None:intergenic 40.0%
CAACAGCCAATTCTTATCTG+AGG + chr1_3:7564573-7564592 None:intergenic 40.0%
CTCAGATAAGAATTGGCTGT+TGG - chr1_3:7564571-7564590 Msa0101070:intron 40.0%
AAGAGGCCAATGATTTCTCA+TGG + chr1_3:7564612-7564631 None:intergenic 40.0%
!!! TGAAGTAGTTTTGGTTTCGC+TGG - chr1_3:7564682-7564701 Msa0101070:intron 40.0%
TTGGTTTCGCTGGTATTCAA+CGG - chr1_3:7564692-7564711 Msa0101070:intron 40.0%
AAGATCTCACCAGGATTTGA+AGG - chr1_3:7564721-7564740 Msa0101070:intron 40.0%
ATTTGAGAGCCTTCAAATCC+TGG + chr1_3:7564733-7564752 None:intergenic 40.0%
TCTACTTGCAATAGTCCACA+TGG - chr1_3:7564846-7564865 Msa0101070:intron 40.0%
TTCGCTGGTATTCAACGGAT+TGG - chr1_3:7564697-7564716 Msa0101070:intron 45.0%
TTCGCTGGTATTCAACGGAT+TGG - chr1_3:7564697-7564716 Msa0101070:intron 45.0%
! CGGATTGGAAAGATCTCACC+AGG - chr1_3:7564712-7564731 Msa0101070:intron 50.0%
! CGGATTGGAAAGATCTCACC+AGG - chr1_3:7564712-7564731 Msa0101070:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 7564051 7565005 7564051 ID=Msa0101070;Name=Msa0101070
chr1_3 mRNA 7564051 7565005 7564051 ID=Msa0101070-mRNA-1;Parent=Msa0101070;Name=Msa0101070-mRNA-1;_AED=0.45;_eAED=0.62;_QI=0|0|0|0.5|1|1|2|0|107
chr1_3 exon 7564998 7565005 7564998 ID=Msa0101070-mRNA-1:exon:2999;Parent=Msa0101070-mRNA-1
chr1_3 exon 7564051 7564366 7564051 ID=Msa0101070-mRNA-1:exon:2998;Parent=Msa0101070-mRNA-1
chr1_3 CDS 7564998 7565005 7564998 ID=Msa0101070-mRNA-1:cds;Parent=Msa0101070-mRNA-1
chr1_3 CDS 7564051 7564366 7564051 ID=Msa0101070-mRNA-1:cds;Parent=Msa0101070-mRNA-1
Gene Sequence

>Msa0101070

ATGTTGAGTTTTGGTTTCGCTGGTATTCAACGGATTGGAAAGATCTCACCAGGATTTGAAGGCTCTCAAATGAATTGGATTGATAGGAATGGAAAATTTCTTGTGTCAAACAAACAAGAATTTGGTTTTGGCTTTGTCACAACTTCAAATGACAACACATTGTTTCTACTTGCAATAGTCCACATGGATAGCACAAAATTGTTTGGACTGCAAATAGAGAATTTCCTGTTTCTAATTCTGATAAGTTTGTGTTTGATGAAAAGGGTAATGCTTTTTTACAAAAAGGAAGAAATTTGGTTTGGTCAACAAATACAAGTGGCATGA

Protein sequence

>Msa0101070

MLSFGFAGIQRIGKISPGFEGSQMNWIDRNGKFLVSNKQEFGFGFVTTSNDNTLFLLAIVHMDSTKLFGLQIENFLFLILISLCLMKRVMLFYKKEEIWFGQQIQVA*